D-stereospecific aminoacyl-tRNA synthetase and method of producing D-stereospecific aminoacyl-tRNA synthetase
10781437 ยท 2020-09-22
Assignee
Inventors
Cpc classification
C12N15/00
CHEMISTRY; METALLURGY
International classification
C12N9/00
CHEMISTRY; METALLURGY
Abstract
A method for one of altering and enhancing the stereospecificity of an enzyme comprising introducing a stereospecific editing domain into the enzyme.
Claims
1. A method for increasing D-stereospecificity of an enzyme comprising: introducing a stereospecific editing domain into the enzyme; wherein the enzyme is Geobacillus stearothermophilus tyrosyl-tRNA synthetase; and the stereospecific editing domain is amino acids 83-275 of a Pyrococcus horikoshii phenylalanine-tRNA synthetase -subunit of Seq. ID. No. 1, that includes one of, L202A, L210A, T221A, T236A, F125A/S211A, L202A/S211A, T221A/S211A, F125A/S202A/S211A, F125A/T221A/S211A, L202A/T221A/S211A, F145A/L202A/T221A/S211A, and L202A/T221A/S211A/T236A mutations, the mutations being between one and four variants; and the steriospecific editing domain being inserted between G161 and I162 on the Geobacillus stearothermophilus tyrosyl-tRNA synthetase of Seq. ID. No. 2.
2. The method of claim 1 wherein the stereospecific editing domain includes one of, L202A, L210A, T221A, T236A, F125A/S211A, L202A/S211A, T221A/S211A, F125A/S202A/S211A, F125A/T221A/S211A, and L202A/T221A/S211A.
3. The method of claim 1 wherein the steriospecific editing domain includes one of F125A/S202A/S211A, F125A/T211A/S211A, and L202A/T211A/S211A.
4. The method of claim 3 wherein the steriospecific editing domain includes F125A/T221A/S211A.
5. The method of claim 1 wherein the stereospecific editing domain has exactly two variants and includes one of F125A/S211A, L202A/S211A, and T221A/S211A.
6. The method of claim 5 wherein the stereospecific editing domain includes F125A/S211A.
7. The method of claim 1 wherein the stereospecific editing domain includes one of F125A/T221A/S211A and F145A/L202A/T221A/S211A.
8. The method of claim 1 wherein the stereospecific editing domain includes one of T221A/S211A, F125A/T211A/S211A, and L202A/T221A/S211A.
9. The method of claim 1 wherein the enzyme causes at least one D-amino acid being used in place of a glycine residue in a translation process.
10. The method of claim 1 wherein the enzyme causes at least one D-amino acid to occupy an inverse conformational space in a translation process.
11. The method of claim 1 wherein the editing domain contains one of F125A/S211A, L202A/S211A, T221A/S211A, F125A/S202A/S211A, F125A/T221A/S211A, and L202A/T221A/S211A.
12. The method of claim 11 wherein the mutations are one of F125A/S211A, L202A/S211A, T221A/S211A, F125A/S202A/S211A, F125A/T221A/S211A, and.
13. A method for increasing Dstereospecificity of an enzyme comprising: introducing a stereospecific editing domain into the enzyme between adjacent glycine and isoleucine amino acids; wherein the enzyme is Geobacillus stearothermophilus tyrosyl-tRNA synthetase; the steriospecific editing domain is amino acids 83-275 of a Pyrococcus horikoshii phenylalanyltRNA synthetase -subunit of Seq. ID. No. 1, having the substitutions F125A and S211A; the steriospecific editing domain being inserted between G161 and I162 on the Geobacillus stearothermophilus tyrosyl-tRNA synthetase of Seq. ID. No. 2; the enzyme causes at least one D-amino acid being used in place of a glycine residue in a translation process; the enzyme causes at least one D-amino acid to occupy an inverse conformational space in a translation process.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
(1) The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate various embodiments of the invention and together with the general description of the invention given above and the detailed description of the drawings given below, serve to explain the principles of the invention. It is to be appreciated that the accompanying drawings are not necessarily to scale since the emphasis is instead placed on illustrating the principles of the invention. The invention will now be described, by way of example, with reference to the accompanying drawings in which:
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DETAILED DESCRIPTION
(16) The present invention will be understood by reference to the following detailed description, which should be read in conjunction with the appended drawings. It is to be appreciated that the following detailed description of various embodiments is by way of example only and is not meant to limit, in any way, the scope of the present invention. In the summary above, in the following detailed description, in the claims below, and in the accompanying drawings, reference is made to particular features (including method steps) of the present invention. It is to be understood that the disclosure of the invention in this specification includes all possible combinations of such particular features, not just those explicitly described. For example, where a particular feature is disclosed in the context of a particular aspect or embodiment of the invention or a particular claim, that feature can also be used, to the extent possible, in combination with and/or in the context of other particular aspects and embodiments of the invention, and in the invention generally. The term comprises and grammatical equivalents thereof are used herein to mean that other components, ingredients, steps, etc. are optionally present. For example, an article comprising (or which comprises) components A, B, and C can consist of (i.e., contain only) components A, B, and C, or can contain not only components A, B, and C but also one or more other components. Where reference is made herein to a method comprising two or more defined steps, the defined steps can be carried out in any order or simultaneously (except where the context excludes that possibility), and the method can include one or more other steps which are carried out before any of the defined steps, between two of the defined steps, or after all the defined steps (except where the context excludes that possibility).
(17) The term at least followed by a number is used herein to denote the start of a range beginning with that number (which may be a range having an upper limit or no upper limit, depending on the variable being defined). For example at least 1 means 1 or more than 1. The term at most followed by a number is used herein to denote the end of a range ending with that number (which may be a range having 1 or 0 as its lower limit, or a range having no lower limit, depending upon the variable being defined). For example, at most 4 means 4 or less than 4, and at most 40% means 40% or less than 40%. When, in this specification, a range is given as (a first number) to (a second number) or (a first number)(a second number), this means a range whose lower limit is the first number and whose upper limit is the second number. For example, 25 to 100 mm means a range whose lower limit is 25 mm, and whose upper limit is 100 mm. The embodiments set forth the below represent the necessary information to enable those skilled in the art to practice the invention and illustrate the best mode of practicing the invention. In addition, the invention does not require that all the advantageous features and all the advantages need to be incorporated into every embodiment of the invention.
(18) Turning now to
(19) Methods and Materials
(20) Materials were obtained from the following sources: TOPO TA cloning kit (Life Technologies, Grand Island, N.Y.), pET30(+) expression vector and BL21(DE3) E. coli cells (EMD Biosciences, Billerica, Miss.), XL2 Blue E. coli cells (Agilent, Santa Clara, Calif.), plasmid DNA Mini I kit (Omega Bio-Tek, Norcross, Ga.), T4 DNA ligase, NdeI, XhoI, and FokI (New England Biolabs, Ipswich, Mass.), HisPur NiNTA resin (Promega, Madison, Wis.), AMP and Biosafe II scintillation cocktail (Research Products International Corporation, Mount Prospect, Ill.), oligonucleotides (Integrated DNA Technologies, Coralville, Iowa), [.sup.14C]L-tyrosine (Moravek, Brea, Calif.), and [.sup.14C] and [.sup.3H]D-tyrosine (American Radiochemicals, St. Louis, Mo.). All other reagents were obtained from VWR International or Fisher Scientific. DNA sequencing was performed by the Arizona State University DNA lab (Tempe, Ariz.). Curve fitting and graphing was performed using GraFit (Eriathicus Software, London, UK) and Kaleidagraph (Syngery Software, Reading, Pa.).
(21) Construction of Expression Plasmids
(22) Construction of an expression plasmid for the TyrRS-FRSed chimera (the Pyrococcus horikoshii phenylalanyl-tRNA synthetase editing domain inserted into the CP1 domain of Geobacillus stearothermophilus tyrosyl-tRNA synthetase) is described in the manuscript included in the APPENDIX. This expression plasmid contains the TyrRS-FRSed coding sequence inserted into the pET30(a)+ vector and is designated pYF1-WT. Activating mutations were introduced into the phenylalanyl-tRNA synthetase editing domain by PCR overlap extension with KOD DNA polymerase to amplify the TyrRS-FRSed coding sequence. Variants with more than one amino acid replacement were introduced using pYF1-S211A as a template and carry the phenylalanyl-tRNA synthetase editing domain variant serine 211 to alanine (S211A). Individual PCR fragments were isolated via Promega SV gel purification and DNA clean up kit prior to recombination by PCR amplification using primers at the 5 and 3 ends of the tyrosyl-tRNA synthetase coding sequence. Following amplification by KOD DNA polymerase, the TyrRS-FRSed coding sequence was purified and A-tailed with Taq DNA polymerase and dATP at 72 C. for 10 minutes. The full length TyrRS-FRSed PCR products were then ligated into pCR2.1-TOPO using a TOPO TA cloning kit and transformed into XL2 Blue cells. Individual colonies were selected and grown overnight in 2 mL 2xYT (16 g/L tryptone, 10 g/L yeast extract, and 5 g/L NaCl) media, followed by PCR amplification to confirm the presence of the TyrRS-FRSed coding sequence. Plasmids from positive clones were isolated using a DNA Mini I kit and the insert was sequenced in its entirety. The TyrRS-FRSed coding sequences were then subcloned into the pET30a(+) expression vector using NdeI and XhoI restriction sites at the 5 and 3 ends respectively, such that the coding sequence is in frame with the coding sequence for a carboxyl-terminal 6His-tag. Single mutation variants are labeled by their mutation according to the P. horikoshii phenylalanyl-tRNA synthetase nomenclature, and multiple mutants are labeled as the combination of single amino acids replacements (e.g. pYF1-S211A represents the expression plasmid for the TyrRS-FRSed chimera containing the S211A activating mutation in the phenylalanyl-tRNA synthetase editing domain). The specific mutations introduced into the phenylalanyl-tRNA synthetase were: F145A, L202A, L210A, S211A, T221A, T236A, F145A/S211A, L202A/S211A, T221A/S211A, F145A/L202A/S211A, F145A/T221A/S211A, L202A/T221A/S211A, F145A/L202A/T221A/S211A, and L202A/S211A/T221A/T236A.
(23) Protein Expression and Purification
(24) S. cerevisiae AMP Deaminase, S. cerevisiae IMP dehydrogenase, T7 RNA polymerase, S. cerevisiae inorganic pyrophosphatase, wild-type G. stearothermophilus tyrosyl-tRNA synthetase, T. thermophilus D-tyrosyl-tRNA deacylase, and the TyrRS-FRSed chimera (both wild-type and editing domain variants were expressed and purified as described in the manuscript included in the APPENDIX. Briefly, purification of the TyrRS-FRSed chimera variants involved expression in E. coli BL21(DE3) cells and purification using NiNTA affinity chromatography. Proteins were isolated to >95% homogeneity based on SDS-PAGE. Extinction coefficients and molecular weights were calculated using the ExPASy ProtParam tool, as described in the manuscript included in the APPENDIX. The TyrRS-FRSed variants were stored in buffer containing 20 mM Tris, pH 7.8, 10 mM -mercaptoethanol, 1 mM EDTA, and 20% glycerol.
(25) In Vitro Transcription and Purification of tRNA.sup.Tyr
(26) The G. stearothermophilus tRNA.sup.Tyr was synthesized by runoff transcription using T7 RNA polymerase using pGFX-WT as a template, as previously described. Briefly, the pGFX-WT, which contains the gene encoding G. stearothermophilus tRNA.sup.Tyr with a 5 T7 promoter and a 3 FokI restriction endonuclease site, was digested with FokI for 2 hours at 37 C. to create template for the in vitro transcription. Transcription reactions contained 100 mM HEPES pH 7.5, 20 mM MgCl.sub.2, 40 mM DTT, 4 mM spermadine, 6.25 mM NTPs, 0.1 mg/mL BSA, 2 U/mL inorganic pyrophosphatase, 0.3 mg/mL T7 RNA polymerase, and 10 U/mL RNase inhibitor, and were incubated at 37 C. for 4 hours. tRNA was isolated using DE52 resin, ethanol precipitated, resuspended with 100 mM HEPES pH 7.5, and annealed by heating tRNA at 85 C. for 5 minutes, adding MgCl.sub.2 to 10 mM, and cooling to room temperature. A nitrocellulose filter-binding aminoacylation assay was used to monitor the incorporation of [.sup.14C]L-tyrosine, which forms L-tyrosyl-tRNA, and was compared to concentrations determined by A.sub.260 (.sub.260=806,100 M.sup.1 cm.sup.1), indicating that more than 70% of tRNA.sup.Tyr can be aminoacylated.
(27) Aminoacylation Assay
(28) Aminoacylation reactions were performed as described in the manuscript included in the APPENDIX. Unless otherwise stated, assays contained 10 mM MgATP, 100 mM Tris pH 7.8, 10 mM MgCl.sub.2, 2 U/mL inorganic pyrophosphatase, and either 10 M [.sup.14C]L-tyrosine or 25 M [.sup.14C]D-tyrosine with variable concentrations of TyrRS and tRNA.sup.Tyr (inorganic pyrophosphatase units are equivalent to those used by NEB for T. litoralis inorganic pyrophosphatase). The aminoacylation potential of tRNA.sup.Tyr was determined using a nitrocellulose filtration assay in which 1 M tRNA (based on A.sub.260 measurements) was incubated with 50 nM wild-type tyrosyl-tRNA synthetase. Samples were quenched by the addition of 3 mL 5% TCA and filtered through BA-85 nitrocellulose discs presoaked with 5% TCA. The nitrocellulose discs were washed three times with cold 5% TCA, dried, and scintillation counted to quantify the amount of [.sup.14C]L-tyrosyl-tRNA bound to the disc.
(29) Monitoring Trans-Editing of the Tyrosyl-tRNA.sup.Tyr Product
(30) Trans-editing of the L- and D-tyrosyl-tRNA products was monitored by preforming the [.sup.14C]L- and D-tyrosyl-tRNA product and monitoring its hydrolysis via a nitrocellulose filter binding assay. [.sup.14C]tyrosyl-tRNA.sup.Tyr was formed by incubating 100 nM TyrRS-WT, 10 mM MgATP, 5 M tRNA.sup.Tyr and either 10 M [.sup.14C]L-tyrosine or 25 M [.sup.14C]D-tyrosine in the presence of 100 mM Tris, pH 7.8, 10 mM MgCl.sub.2, and 2 U/mL inorganic pyrophosphatase at 25 C. for either 20 minutes (L-tyrosine) or 40 minutes (D-tyrosine). The [.sup.14C]tyrosyl-tRNA product was purified by two phenol:chloroform:isoamylalcohol (50:49:1) extractions, followed by the addition of 0.5 volumes of 7.5 M ammonium acetate and ethanol precipitation. Dried pellets were resuspended in 100 mM HEPES, pH 7.5, 10 mM MgCl.sub.2, and 5 L aliquots were removed, spotted onto nitrocellulose filters, and scintillation counted to determine the concentration of [.sup.14C]tyrosyl-tRNA.sup.Tyr. Trans-editing activity was monitored by incubating [.sup.14C]tyrosyl-tRNA (2-5 M) with 50-250 nM TyrRS-WT or TyrRS-FRSed in the presence of 100 mM HEPES, pH 7.5, and 10 mM MgCl.sub.2. 10 L aliquots were removed and quenched by the addition to 3 mL 5% TCA, filtered through nitrocellulose discs to separate [.sup.14C]tyrosyl-tRNA.sup.Tyr from [.sup.14C]tyrosine, and scintillation counted to quantify the amount of [.sup.14C]tyrosyl-tRNA.sup.Tyr bound to the disc. The data was plotted (cpm versus time) and fit a first order decay equation with floating endpoint:
[TyrRS-tRNA].sub.t=[TyrRS-tRNA].sub.0e.sup.k.sup.
where [TyrRS-tRNA].sub.t is the concentration of [.sup.14C]-labeled tyrosyl-tRNA.sup.Tyr at time t, [TyrRS-tRNA].sub.0 is the initial concentration of [.sup.14C]-labeled tyrosyl-tRNA.sup.Tyr (i.e. at t=0), k.sub.1 is the rate of hydrolysis, t is the time in seconds, and [TyrRS-tRNA].sub. is the final concentration of [.sup.14C]-labeled [TyrRS-tRNA] (i.e. at an infinite time point).
(31) Monitoring Editing of Tyrosyl-tRNA Produced In Situ
(32) The editing activity of the TyrRS-FRSed variants was monitored using a continuous spectrophotometric assay in which tyrosyl-tRNA is produced in situ. This assay is based on a continuous spectrophotometric tyrosyl-tRNA synthetase assay in which the production of AMP is coupled to the reduction of NAD.sup.+, resulting in an increase in absorbance at 340 nm (.sub.340=6220 M.sup.1 cm.sup.1). To monitor editing activity, wild-type tyrosyl-tRNA synthetase is used to generate tyrosyl-tRNA in situ, while the TyrRS-FRSed variant hydrolyzes the tyrosyl-tRNA product, regenerating the free tyrosine and tRNA substrates. If the wild-type tyrosyl-tRNA synthetase and TyrRS-FRSed concentrations are adjusted such that hydrolysis of tyrosyl-tRNA is the rate-limiting step in the reaction, then the increase in absorbance at 340 nm will correspond to the editing activity of the TyrRS-FRSed variant. Specifically, the spectrophotometric TyrRS-FRSed editing assay contained 50 mM Tris, pH 7.78, 10 mM KCl, 10 mM MgCl.sub.2, 0.1 mM dithiothreitol, 10 mM MgATP, 5 mM NAD.sup.+, 160 nM AMP deaminase, 640 nM IMP dehydrogenase, 2 U/mL inorganic pyrophosphatase, 0.5 mM tyrosine, 0.5-1 M TyrRS-WT and 50-250 nM TyrRS-FRSed, and variable concentrations of tRNA.sup.Tyr. All substrate solutions were adjusted to pH 7.0 prior to use. Assays were either 100 or 200 L in volume (corresponding to 0.28 and 0.56 cm pathlengths, respectively) and were performed in 96-well plates at 25 C. Changes in absorbance at 340 nm were monitored using a Biotek Synergy 4 plate reader. The changes in absorbance were plotted against time and fit to a linear equation to determine the initial rate (v.sub.o) for each substrate concentration.
(33) Initial rates (A.sub.340/sec) were converted to mol/sec Beer's law (A=bc, where A is the absorbance is the molar extinction coefficient, and b is the sample path length, and c is the molar concentration). Rates were plotted against substrate concentration and the data was fit to the Michaelis-Menten equation to determine the K.sub.M and V.sub.max values:
v.sub.0=V.sub.max[S]/(K.sub.M+[S])(Equation 2)
where v.sub.0 is the initial rate, V.sub.max is the maximum rate, [S] is the substrate concentration, and K.sub.M is the Michaelis constant for the substrate.
(34) The k.sub.cat values were calculated from V.sub.max using the equation 3:
V.sub.max=k.sub.cat[E](Equation 3)
where [E] is the total enzyme concentration.
(35) Calculation of Relative Free Energies
(36) The relative free energies for the binding of L-tyrosyl-tRNA.sup.Tyr to the phenylalanyl-tRNA synthetase editing domain of the TyrRS-FRSed chimera and subsequent formation of the TyrRS-FRSed[Tyr-tRNA].sup. transition state were calculated from K.sub.M and k.sub.cat values determined in the spectrophotometric assay, assuming standard states of 1 M tyrosyl-tRNA as follows:
G.sub.ETyr-tRNA=RT ln(K.sub.M)(Equation 4)
G.sub.E[Tyr-tRNA]=RT ln(k.sub.BT/h)RT ln(k.sub.cat/K.sub.M)(Equation 5)
where G.sub.ETyr-tRNA and G.sub.[ETyr-tRNA] are the Gibbs free energy values for TyrRS-FRSedTyr-tRNA and TyrRS-FRSed[Tyr-tRNA].sup. complexes relative to that of the unliganded TyrRS-FRSed, R is the gas constant, T is the absolute temperatures, k.sub.B is the Boltzmann constant, h is Planck's constant, and denote noncovalent and covalent bonds, respectively. Subtracting equation 7 from equation 8 results in the following equation:
G.sub.[ETyr-tRNA]=RT ln(k.sub.BT/h)RT ln(k.sub.cat)(Equation 6)
which was used to calculate the Gibbs activation energy.
(37) Calculation of Interaction Energies
(38) Synergistic and antisynergistic interactions between editing site resides were quantified by calculating the Gibbs free energies of interaction using the convention described previously (First and Fersht, 1995). Briefly, this involves calculating the binding energy for each complex relative to the free energy of the unliganded enzyme (i.e. G.sub.E=0) as described above and in (Wells and Fersht, 1986). The apparent binding energy for side chain that has been mutated (G.sub.app) is calculated by subtracting the free energy change for formation of the complex by the wild-type enzyme from that of the variant enzyme (e.g. G.sub.app=G.sub.ETyr-tRNA(mutant)G.sub.ETyr-tRNA(wild-type), where G.sub.ETyr-tRNA for the mutant and wild-type enzymes are calculated using equation 4, as described above). To quantify the effect that the interaction between two residues has on the binding of a particular complex, double mutant free energy cycles are constructed and the pairwise interaction energy (.sup.2G.sub.int) is calculated as described by Horovitz and Fersht (1992), using the following equation:
.sup.2G.sub.int=G.sub.2G.sub.1(Equation 7)
where G.sub.1 is the free energy change for the addition of one sidechain in the absence of the other side chain and G.sub.2 is the free energy for the addition of both side chains simultaneously. Similarly, the ternary coupling interaction energies (i.e. the effect of a third side chain on the previous pairwise coupling interaction between the first two residues) can be calculated using the following equation:
.sup.3G.sub.int=G.sub.3G.sub.2+G.sub.1(Equation 8)
where G.sub.1 and G.sub.2 are as described above and G.sub.3 is the free energy for adding all three sidechains simultaneously (Horovitz and Fersht, 1992).
(39) Propagation of Errors
(40) Errors in primary data were determined by calculating the standard error, based on at least three independent experiments. For subsequent calculations (e.g. k.sub.cat/K.sub.m, G.sup.o, .sup.2G.sup.o.sub.int, etc.), errors were propagated using the equations shown below.
(41) For addition or subtraction (x+y=R):
dR=(dx).sup.2+(dy).sup.2(Equation 9)
where x and y are the variables (e.g. k.sub.cat, K.sub.m, G.sup.o, etc.), R is the final result, and dx, dy, and dR are the errors in x, y, and R.
For multiplication or division of two variables (x.Math.y=R):
dR=|R|(dx/x).sup.2+(dy/y).sup.2(Equation 10)
where x, y, R, dx, dy, and dR are defined above.
For multiplication or division by a constant value (c.Math.x=R):
dR=|c|.Math.dx(Equation 11)
where c is a constant and x, R, and dR are defined above.
For conversion to a natural logarithm (ln(x)=R):
dR=dx/x(Equation 12)
where x, R, dx, and dR are defined above.
(42) Monitoring the Competition Between L- and D-Tyrosine
(43) A competition assay was used to quantify the effect that introducing the phenylalanyl-tRNA synthetase editing domain into tyrosyl-tRNA synthetase had on the stereospecificity of the enzyme. Competition assays contained 10 mM MgATP, 100 mM Tris pH 7.8, 10 mM MgCl.sub.2, 1 U/mL inorganic pyrophosphatase, 10 M tRNA, 50-200 nM TyrRS-FRSed, 30 M [.sup.14C]L-tyrosine, and variable concentrations of [.sup.3H]D-tyrosine (0 to 120 M). Assays were incubated at 25 C. for 15 minutes, at which point 20 L aliquots were removed and were quenched by the addition to 3 mL 5% TCA. This 15 minute time point was selected based on the observation that this time point was within the linear phase of the reaction for both the TyrRS-WT and TyrRS-FRSed enzymes.
(44) Following quenching of the reaction, the aliquots were filtered through nitrocellulose filters that had been presoaked in 5% TCA. The nitrocellulose filters were washed three times with 3 mL of ice-cold 5% TCA, dried, and subjected to scintillation counting to quantify the amount of [.sup.14C]L-tyrosyl-tRNA and [.sup.3H]D-tyrosyl-tRNA present. The Beckman LS-6500 scintillation counter was calibrated using commercial standards (Beckman Coulter), and quench curves for the dual label counting were setup by spotting [.sup.14C]L-tyrosine and [.sup.3H]D-tyrosine onto nitrocellulose filters, adding 5.5 mL Biosafe II scintillation cocktail to 7 mL scintillation vials, and adding increasing amounts of chloroform to the vials. Counting efficiencies for [.sup.3H]D-tyrosine were near 40%, while efficiencies for [.sup.14C]L-tyrosine were greater than 85%, compared to the calculated DPMs. After normalizing for counting efficiency, the data were plotted as [D-Tyr-tRNA]/[L-Tyr-tRNA] vs. [D-tyrosine] and fit to a linear equation.
(45) Theory
(46) For all naturally occurring amino acids except glycine and alanine, substitution of the side chain by alanine effectively removes that amino acid side chain. This allows one to use alanine substitution to determine the role that specific amino acids play in the properties of a protein (e.g. enzyme catalysis, protein folding, etc.). Alanine substitutions usually represent a relatively conservative change as it is less likely that adventitious effects will result from removal of a side chain than would be the case if the side chain was replaced with another side chain that alters its chemical or steric properties. The effect that the side chain has on catalysis (or another property, such as stability) can be quantified by converting the rate and dissociation (or Michaelis) constants to standard Gibbs free energy values for the wild type and alanine substituted enzymes. This is done by calculating the free energy change for the wild type and alanine substituted protein variants (e.g. using G.sup.o=RTInK.sub.eq or an analogous equation) and then subtracting the standard free energy change of the alanine variant from that of the wild type protein (i.e. G.sup.o.sub.0.fwdarw.i where i is the wild type side chain and 0 is the alanine substitution).
(47) As interactions between amino acids play an essential role in a protein's properties, it is of interest to quantify not only the effect that a single amino acid side chain has on the protein, but also the effect due to the interaction between amino acid side chains. This can be done by introducing alanine substitutions at multiple positions and quantifying the effect that these substitutions have using multimutant free energy cycles. For example, a double mutant free energy cycle can be used to quantify the role that the interaction between two amino acids plays in a protein's property (e.g. enzyme catalysis). This is shown in
(48) Comparing the transformations at the top and bottom of the double mutant free energy cycle (i.e. 0,0.fwdarw.i,0 and 0,j.fwdarw.i,j, respectively) one can see that the only difference between the two transformations is the absence (top) or presence (bottom) of the second side chain (j). In other words, the difference between the standard free energies for the two transformations is due to the standard free energy of interaction between the i and j side chains (i.e. .sup.2G.sup.o.sub.int). The value of .sub.2G.sup.o.sub.int can be calculated by subtracting the standard free energy changes for opposite sides of the cycle (
(49) Multimutant free energy cycles can be extended to higher orders to quantify interactions between three or more amino acid side chains. This is shown in
(50)
where n is the total number of side chains being added, r is the number of additions in a given molecule (rn), n.sub.r are the binomial coefficients, and represents the different possible combinations for each r value.
(51) Free energy analysis of amino acid substitutions that result in a more active protein is similar to that used to monitor deleterious substitutions, except that the frame of reference is reversed. In other words, for substitutions that increase the activity (or other property) of a protein, the most active variant is located in the bottom right corner of the cycle (i.e. where the wild type protein would normally be located). Using this convention, the .sup.2G.sup.o.sub.int<0 for interactions that have a synergistic effect on the activity (or other property) of the protein and .sup.2G.sup.o.sub.int>0 for interactions that have an antisynergistic effect.
(52) RESULTSEditing Domain Mutations Activate Hydrolysis of L-Tyrosyl-tRNA, but not D-Tyrosyl-tRNA in the TyrRS-FRSed Variant.
(53) Alanine substitutions near the hydrolytic active site of the P. horikoshii phenylalanyl-tRNA synthetase editing domain have been previously implicated in a loss of specificity of the editing domain. Since the insertion of this editing domain into tyrosyl-tRNA synthetase altered the stereospecificity of tyrosyl-tRNA synthetase, the inventors hypothesized that introducing phenylalanyl-tRNA synthetase editing domain variants containing these activating amino acid replacements into tyrosyl-tRNA synthetase, while leaving the synthetic active site of tyrosyl-tRNA synthetase unaffected, would further enhance the stereospecificity of the TyrRS-FRSed enzyme.
(54) Six amino acid substitutions in the editing domain of P. horikoshii phenylalanyl-tRNA synthetase, F145A, L202A, L210A, S211A, T221A, and T236A (collectively the Yokoyama mutations) were introduced into the phenylalanyl-tRNA synthetase editing domain in the TyrRS-FRSed variant, both individually and in various combinations, as shown in Table 1. The inventors included the S211A substitution in all of the TyrRS-FRSed variants containing multiple activating substitutions. To determine the effect that these substitutions have on hydrolysis of L- and D-tyrosyl-tRNA.sup.Tyr, a trans-editing assay was used. In this assay, each of the TyrRS-FRSed variants is incubated with [.sup.14C]L-tyrosyl-tRNA.sup.Tyr at 25 C. At various time points, aliquots are removed, the hydrolysis reaction is quenched, and the samples are filtered through nitrocellulose discs to capture [.sup.14C]L-tyrosyl-tRNA.sup.Tyr that has not been hydrolyzed. First order rate constants for hydrolysis of L-tyrosyl-tRNA.sup.Tyr by the TyrRS-FRSed editing domain variants are shown in Table 1 and
(55) Steady-State Kinetic Analysis of L-Tyrosyl-tRNA.sup.Tyr Hydrolysis by the TyrRS-FRSed Editing Domain Variants.
(56) While the trans-editing assay was useful for preliminary screening of the TyrRS-FRSed editing domain variants, using it for detailed kinetic analyses of each of the variants is both time-consuming and expensive. As an alternative to the trans-editing assay, the inventors developed a continuous spectrophotometric editing assay based on a high-throughput tyrosyl-tRNA synthetase assay that was recently developed in their laboratory (provisional patent application #62/060,059). Tyrosyl-tRNA synthetase catalyzes the aminoacylation of tRNA using a two-step mechanism. In the first step, tyrosine is activated by ATP, forming an enzyme-bound tyrosyl-adenylate intermediate (TyrRSTyr-AMP, where and represent noncovalent and covalent bonds, respectively). In the second step of the reaction, the tyrosyl moiety is transferred to the 3 end of tRNA.sup.Tyr and the Tyr-tRNA.sup.Tyr and AMP products are released from the enzyme. To monitor tyrosyl-tRNA synthetase activity, the production of AMP is coupled to the reduction of NAD.sup.+ through the actions of AMP deaminase (which converts AMP to IMP) and IMP dehydrogenase (which uses NAD.sup.+ to oxidize IMP to XMP). As reduction of NAD.sup.+ to NADH results in an increase in absorbance at 340 nm (.sub.340.sup.NADH=6220 M.sup.1 cm.sup.1), tyrosyl-tRNA synthetase activity can be monitored spectrophotometrically. To increase the sensitivity and decrease the cost of the tyrosyl-tRNA synthetase assay, cyclodityrosine synthase is used to regenerate the tRNA.sup.Tyr substrate in situ. Cyclodityrosine synthase converts two molecules of L-tyrosyl-tRNA.sup.Tyr to cyclodityrosine and two molecules of tRNA.sup.Tyr.
(57) Turning next to
(58) As shown in Table 1,
(59) Much to the surprise of the inventors, introducing additional mutations in the presence of the S211A substitution decreased the affinity of the editing domain for L-tyrosyl-tRNA.sup.Tyr relative to that of TyrRS-FRSed containing only the S211A substitution. However, the TyrRS-FRSed variants containing multiple mutations still had a higher affinity for L-tyrosyl-tRNA.sup.Tyr than wild type TyrRS-FRSed. As shown in Table 1, analysis of k.sub.cat values for L-tyrosyl-tRNA hydrolysis indicated that all of the single mutants had higher values than the wild-type TyrRS-FRSed, with the F145A and L202A variants having the largest k.sub.cat values of all the single mutants. Introducing a second mutation in the TyrRS-FRSed-S211A variant, resulted in an increase in k.sub.cat values, with the F145A/S211A double mutant displaying the highest k.sub.cat value. Introducing additional substitutions beyond the second mutation decreased the k.sub.cat value for the editing domain (although the k.sub.cat values for the triple and quadruple mutants were still higher than that of the wild-type TyrRS-FRSed).
(60) In the APPENDIX manuscript, the inventors showed that the wild-type phenylalanyl-tRNA synthetase editing domain is stereospecific, catalyzing the hydrolysis of L-tyrosyl-tRNA.sup.Tyr, but not D-tyrosyl-tRNA.sup.Tyr. To determine whether this stereospecificity is lost in the editing domain variants, time course assays were performed using saturating D-tyrosine and tRNA.sup.Tyr concentrations (300 M and 6 M, respectively) using the continuous spectrophotometric phenylalanyl-tRNA synthetase editing assay. As shown in
(61) Free Energy Analysis of Editing Domain Variants.
(62) To quantify the effect that each of the editing domain variants had on the L-tyrosyl-tRNA.sup.Tyr hydrolysis reaction, the K.sub.m.sup.L-Tyr-tRNA and k.sub.cat values were used to calculate relative standard free energy values for the TyrRS-FRSedL-Tyr-tRNA and TyrRS[L-Tyr-tRNA].sup. complexes (where .sup. indicates the transition state). These values are shown, along with the relative standard free energy for activation in Table 3. Of the single variants, F145A and S211A had the largest effect on the relative standard free energy value for the TyrRSL-Tyr-tRNA complex (G.sup.o.sub.TyrRSTyr-tRNA), increasing the stability of this complex by 3.5 and 4.0 kJ/mol, relative to that of the wild-type TyrRS-FRSed, respectively. The F145A and L202A variants had the largest effect on the activation energies, decreasing the activation energies of these variants by 3.7 and 4.0 kJ/mol, respectively. The variants that had the largest effect on the free energy of the transition state complex (G.sup.o.sub.TyrRS[Tyr-tRNA].sub.
(63) In general, the relative standard free energies of the TyrRSL-Tyr-tRNA and TyrRS[L-Tyr-tRNA].sup. complexes of the multiple alanine variants were more stable and had lower activation energies than the single variants. This was seen most clearly with the double variants, which stabilized the TyrRSL-Tyr-tRNA and TyrRS[L-Tyr-tRNA].sup. complexes and lowered the activation energy to a greater extent than higher order (i.e. triple and quadruple) variants. Furthermore, the ranges of the relative free energy values for the TyrRSL-Tyr-tRNA and TyrRS[L-Tyr-tRNA].sup. complexes, as well as the activation energy, were significantly lower for the multiple variants than they were for the single variants, suggesting that introducing additional variants tends to dampen the effects of the single variants. For example, the range for G.sup.o.sub.TyrRSTyr-tRNA was 4.0 kJ/mol for the single variants and 1.6 kJ/mol for the multiple variants. Furthermore, the effect that each substitution had on the binding of Tyr-tRNA tended to correlate with the effect that the substitution had on the transition state (
(64) Quantifying Standard Free Energies of Interaction Between the Editing Domain Side Chains.
(65) Standard free energies of interaction between side chains can be quantified using double mutant free energy cycles. Double mutant free energy cycles can be constructed for each step along the reaction pathway, allowing the effect that energetic coupling has on the stability of each complex to be determined. For example, the double mutant free energy cycle shown in
(66) Extending the free energy of interaction analysis to higher order interactions, one can see that in the presence of S211A, the ternary interaction between the F145A, L202A, and T221A activating mutations is antisynergistic (i.e. destabilizing) for the TyrRSL-Tyr-tRNA (compare the .sup.2G.sup.o.sub.int values in
(67) Editing Domain Variants Switch the Stereospecificity of the TyrRS-FRSed Variant.
(68) To determine the effect that increasing the activity of the editing domain had on the stereospecificity of the TyrRS-FRSed variant, four editing domain variants were selected for further analysis: wild-type TyrRS-FRSed, an editing defective variant (N217A), and two variants containing activating mutations in the editing domain (T221A and F145A/S211A). To quantify the stereospecificity of the TyrRS-FRSed editing domain, a competition assay was performed. In this assay, the TyrRS-FRSed variants were incubated in the presence of 10 mM MgATP, 10 M tRNA.sup.Tyr, 30 M [.sup.14C]L-tyrosine, and varying amounts of [.sup.3H]D-tyrosine, and the formation of L- and D-tyrosyl-tRNA.sup.Tyr was monitored by determining the amount of [.sup.14C]L-tyrosine and [.sup.3H]D-tyrosine incorporated into the Tyr-tRNA product. This competition assay has previously been used to demonstrate that ability of D-tyrosine to compete with L-tyrosine in the aminoacylation reaction is 2-fold higher for the TyrRS-FRSed variant than it is for the wild type tyrosyl-tRNA synthetase, as shown in the Appendix manuscript.
(69) Turning to
(70) DISCUSSIONThe insertion of the wild type P. horikoshii phenylalanyl-tRNA synthetase editing domain into G. stearothermophilus tyrosyl-tRNA synthetase was insufficient to switch the stereospecificity of the enzyme to prefer D-tyrosine over L-tyrosine at equimolar concentrations. The insertion of this editing domain, despite being stereospecific for L-tyrosyl-tRNA.sup.Tyr hydrolysis, showed only modest difference in stereospecificity compared to the wild-type G. stearothermophilus tyrosyl-tRNA synthetase.
(71) To improve the stereospecificity of the TyrRS-FRSed chimera, the inventors sought to increase the hydrolytic activity of the inserted phenylalanyl-tRNA synthetase using single and multiple amino acid replacements. The examined enzyme variants showed increased editing activity under steady state conditions compared to the wild-type TyrRS-FRSed chimera. These activating enzyme variants showed up to 5-fold increase in the affinity for L-tyrosyl-tRNA and up to 7.5-fold increase in the rate of hydrolysis. Replacement with other amino acids could provide an increased activity, if not understanding, at the role that these amino acid residues play in substrate recognition and hydrolytic activity. It was originally suggested these enhancing variants showed an increase in product release, but this effect alone does not agree with the observed increases in L-tyrosyl-tRNA.sup.Tyr affinity.
(72) One of these double amino acid replacements, the TyrRS-FRSed-F145A/S211A, showed the largest increase in the activity of the inserted phenylalanyl-tRNA synthetase editing domain. This double variant showed a preference for D-tyrosine at equimolar concentrations of L- and D-tyrosine with a strong preference at higher concentrations of D-tyrosine.
(73) This engineered D-tyrosine specific tyrosyl-tRNA synthetase may be used for the selection of EF-1 and ribosome variants that allow the efficient incorporation of D-amino acids into protein in archaea. Specifically, the TyrRS-FRSed variant is introduced into archaeal or eukaryotic cells along with a variant of bacterial tRNA.sup.Tyr in which the tyrosine anticodon is replaced by an anticodon that recognizes a stop codon in mRNA (e.g. UAG). This allows incorporation of D-tyrosine at positions in the mRNA sequence containing the stop codon. A reporter coding sequence (e.g. chloramphenicol, green fluorescence protein, or luciferase) containing an internal stop codon is used to select for EF-1 or ribosome variants that increase the efficiency of incorporating D-tyrosine into the reporter protein.
(74) Alternatively, the phenylalanyl-tRNA synthetase editing domain variants can be inserted into an archaeal or eukaryotic tyrosyl-tRNA synthetase (e.g. Methanococcus jannaschii tyrosyl-tRNA synthetase) and the resulting expression plasmid can be transfected into a bacterial host cell (e.g. Escherichia coli) along with a variant of the archaeal or eukaryotic tRNA.sup.Tyr in which the tyrosine anticodon is replaced by an anticodon that recognizes a stop codon in mRNA (e.g. UAG). This allows incorporation of D-tyrosine at positions in the mRNA sequence containing the stop codon. A reporter coding sequence (e.g. chloramphenicol, green fluorescence protein, or luciferase) containing an internal stop codon is used to select for EF-Tu or ribosome variants that increase the efficiency of incorporating D-tyrosine into the reporter protein.
(75) Such a system to site-specifically incorporate D-tyrosine, or other D-amino acids, is useful for screening new peptide therapeutics within cells. Synthesized D-amino acid containing peptides have been shown to block extracellular protease activity and protein-protein interactions by acting as a non-hydrolysable mimetic; however, internalization of these peptide therapeutics has proven challenging as normal translocation motifs are less effective when they contain a D-amino acid. Introducing the necessary machinery for the orthogonal incorporation of D-amino acids into cells with a library of small peptides allows for the discovery of stable peptides of value without exogenously adding the library of in vitro synthesized peptides.
(76) Incorporating D-amino acids into peptides and proteins has a number of advantages. Peptides containing D-amino acids are resistant to proteolysis, making them potential inhibitors of proteases. These D-peptides can be used therapeutically (for example, in the treatment of AIDS by inhibiting HIV protease). Furthermore, since they are not processed by proteolytic enzymes, D-peptides and proteins are not efficiently recognized by the immune system. Both their resistance to proteolysis and lack of recognition by the immune system increases the physiological half-life of D-peptides and proteins, providing a therapeutic advantage over L-peptides and proteins.
(77) In addition to being resistant to proteolysis, these D-amino acid containing peptides can occupy inverse conformational space when compared to all L-amino acid containing peptides and proteins. The site specific inclusion of a D-amino acid into a polypeptide, whether randomly generated, rationally designed, or molecularly modeled peptide, has proven very important for the stabilization of the alternate conformations and unlocks protein engineering by introducing a fundamental change in the protein backbone, unlike other unnatural amino acids.
(78) In proteins, the amino acid glycine is often used in turns as it is able to adopt conformations that are unavailable to L-amino acids. In contrast, D-amino acids are able to replace these glycine residues, as they are able to adopt the required conformation for the turn. Replacing the glycine residues with D-amino acids reduces the conformational entropy of the turn, increasing the stability of the protein. This approach can be used to create hyperstable proteins. Such proteins have a number of industrial applications (e.g. stabilization of enzymes used in laundry detergents).
(79) The inventors have demonstrated that editing domains can be used to alter the stereospecificity of a specific class of enzymesnamely the aminoacyl-tRNA synthetases. The same approach can be used to alter or improve the stereospecificity of any enzyme. For example, aldolases catalyze the formation of carbon-carbon bonds via the aldol reaction, providing potential tools for synthesizing structurally complex chiral molecules. This potential for use in synthetic chemistry has driven efforts to alter the stereospecificity of aldolases. In particular, the stereospecificity of tagatose 1,6-bisphosphate aldolase, which catalyzes the synthesis of tagatose 1,6-bisphosphate from dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, has been altered using directed evolution methods (tagatose 1,6-bisphosphate is the stereoisomer of fructose 1,6-bisphosphate). These efforts produced a variant of tagatose 1,6-bisphosphate aldolase that has a 4:1 preference for the production of fructose 1,6-bisphosphate over of tagatose 1,6-bisphosphate (wild type of tagatose 1,6-bisphosphate aldolase has a 99:1 preference for the production of tagatose 1,6-bisphosphate). The 4:1 preference of the of tagatose 1,6-bisphosphate aldolase variant could be further enhanced by introducing an editing domain (in cis or in trans) that hydrolyzes the undesired of tagatose 1,6-bisphosphate product, either regenerating the original substrates of the reaction (i.e. dihydroxyacetone phosphate and glyceraldehyde 3-phosphate) or releasing hydrolysis products that can readily be separated from the desired product (i.e. fructose 1,6-bisphosphate). Although this specific example may not have immediate industrial applications, it demonstrates through a further embodiment the value of the approach described by the inventors for altering and/or improving the stereospecificity of an enzyme. It is understood that description of the various enzymes in general and tRNA synthetases in particular include the enzyme's variants.
(80) Tables:
(81) TABLE-US-00001 TABLE 1 Steady state kinetic analysis of editing activity.sup.1 K.sub.M.sup.Tyr-tRNA k.sub.cat k.sub.cat/K.sub.M TyrRS-FRSed Variant (M) (s.sup.1) (s.sup.1 M.sup.1) WT 8 (1) 0.32 (0.03) 0.040 (0.006) F145A 1.9 (0.1) 1.4 (0.1) 0.74 (0.07) L202A 4.6 (0.8) 1.6 (0.4) 0.3 (0.1) L210A 6.1 (0.8) 0.73 (0.08) 0.12 (0.02) S211A 1.6 (0.3) 0.9 (0.2) 0.6 (0.2) T221A 4 (2) 0.6 (0.2) 0.2 (0.1) T236A 7.7 (0.8) 0.46 (0.05) 0.06 (0.01) F145A/S211A 2.6 (0.2) 2.4 (0.3) 0.9 (0.1) L202A/S211A 2.6 (0.7) 1.7(0.2) 0.7 (0.2) S211A/T221A 2.5 (0.1) 1.5 (0.2) 0.6 (0.1) F145A/L202A/S211A 4.4 (0.8) 1.4 (0.4) 0.3 (0.1) F145A/S211A/T221A 2.7 (0.8) 1.6 (0.4) 0.6 (0.2) L202A/S211A/T221A 2.7 (0.7) 1.6 (0.1) 0.6 (0.2) F145A/L202A/S211A/ 4.1 (0.5) 1.15 (0.09) 0.28 (0.04) T221A L202A/S211A/T221A/ 4.7(0.9) 0.9 (0.1) 0.19 (0.04) T236A .sup.1Standard errors are shown in parentheses
(82) TABLE-US-00002 TABLE 2 Comparison of [.sup.14C]L-Tyr-tRNA.sup.Tyr hydrolysis results with predicted results based on the k.sub.cat and K.sub.m values determined by spectrophotometric editing assay Variant Observed rate (s.sup.1) Predicted Rate.sup.1 TyrRS-FRSed (WT) 0.0008 (0.0003) 0.00069 TyrRS-FRSed-T221A 0.003 (0.001) 0.0034 TyrRS-FRSed-F145A/S221A 0.0099 (0.0005) 0.012 .sup.1The predicted rate is calculated based on the k.sub.cat and K.sub.m values from Table 1, assuming L-Tyr-tRNA.sup.Tyr and TyrRS-FRSed concentrations of 1.7 M and 12.5 nM concentrations, respectively. Standard error values are shown in parentheses.
(83) TABLE-US-00003 TABLE 3 Relative free energies for TyrRS-FRSed variants.sup.1 G.sub.TyrRSTyr-tRNA G.sub.TyrRS[Tyr-tRNA] Activation Energy TyrRS-FRSed Variant (kJ mol.sup.1) (kJ mol.sup.1) (kJ mol.sup.1) WT 29.1 (0.3) 115.2 (0.4) 144.3 (0.5) F145A 32.6 (0.1) 108.0 (0.2) 140.6 (0.3) L202A 30.4 (0.4) 109.8 (0.8) 140.3 (0.9) L210A 29.8 (0.3) 112.5 (0.4) 142.2 (0.5) S211A 33.1 (0.4) 108.6 (0.7) 141.7 (0.8) T221A 31 (1) 112 (1) 143 (2) T236A 29.2 (0.2) 114.2 (0.4) 143.4 (0.4) F145A/S211A 31.9 (0.2) 107.4 (0.4) 139.3 (0.4) L202A/S211A 31.9 (0.6) 108.3 (0.7) 140.1 (0.9) S211A/T221A 32.0 (0.1) 108.5 (0.3) 140.4 (0.4) F145A/L202A/S211A 30.6 (0.4) 110.1 (0.8) 140.6 (0.9) F145A/S211A/T221A 31.8 (0.6) 109 (1) 140 (1) L202A/S211A/T221A 31.8 (0.6) 108.5 (0.7) 140.3 (0.9) F145A/L202A/S211A/T221A 30.7 (0.3) 110.4 (0.4) 141.1 (0.5) L202A/S211A/T221A/T236A 30.4 (0.4) 111.3 (0.5) 141.7 (0.7) .sup.1Free energies are calculated relative to the unliganded enzyme. Standard error values are shown in parentheses.
(84) TABLE-US-00004 TABLE S1 Comparison of [.sup.14C]L-Tyr-tRNA.sup.Tyr hydrolysis results with predicted results based on the k.sub.cat and K.sub.m values determined by spectrophotometric editing assay Variant Observed Rate (s.sup.1) Predicted Rate (s.sup.1) WT 0.00073 0.00069 N217A N/A N/A F145A 0.00628 0.00878 L202A 0.00568 0.00531 S211A 0.00616 0.00549 T221A 0.00255 0.00336 L210A 0.00264 0.0018 T236A 5.4E05 0.00095 F145A/S211A 0.01042 0.01196 L202A/S211A 0.00471 0.00874 S211A/T221A 0.00715 0.00706 F145A/L202A/S211A 0.00585 0.00396 F145A/S211A/T221A 0.00956 0.00693 L202A/S211A/T221A 0.00565 0.00767 F145A/L202A/S211A/T221A 0.005 0.00418 L202A/S211A/T221A/T236A 0.00907 0.003 .sup.1The predicted rate is calculated based on the k.sub.cat and K.sub.m values from Table 1, assuming L-Tyr-tRNA.sup.Tyr and TyrRS-FRSed concentrations of 1.7 M and 12.5 nM concentrations, respectively. Observed rate values are taken from a single experimental replicate.
(85) TABLE-US-00005 TABLE S2 Free Energies of Coupling in the EL-Tyr-tRNA Complex Coupling analyzed Effect of the coupling (additional alanine substitutions .sup.2G.sub.int on the stability of shown in parentheses) (kJ/mol) the EL-Tyr-tRNA Complex F145A:S211A 4.7 Destabilizing L202A:S211A 2.5 Destabilizing S211A:T221A 3.0 Destabilizing F145A:L202A (S211A) 0.1 Negligible F145A:T221A (S211A) 1.0 Stabilizing L202A:T221A (S211A) 1.0 Stabilizing F145A:L202A (S211A/T221A) 0.9 Destabilizing F145A:T221A (L202A/S211A) 0.2 Negligible L202A:T221A (F145A/S211A) 0.2 Negligible
(86) TABLE-US-00006 TABLE S3 Free Energies of Coupling in the E[L-Tyr-tRNA].sup. Complex Coupling analyzed Effect of the coupling (additional alanine substitutions .sup.2G.sub.int on the stability of the shown in parentheses) (kJ/mol) XE[L-Tyr-tRNA].sup. Complex F145A:S211A 6.0 Destabilizing L202A:S211A 5.1 Destabilizing S211A:T221A 3.1 Destabilizing F145A:L202A (S211A) 3.0 Destabilizing F145A:T221A (S211A) 1.7 Destabilizing L202A:T221A (S211A) 0.3 Negligible F145A:L202A (S211A/T221A) 1.4 Destabilizing F145A:T221A (L202A/S211A) 0.1 Negligible L202A:T221A (F145A/S211A) 1.3 Stabilizing
(87) TABLE-US-00007 TABLE S4 Free Energies of Coupling for the Activation Energy Coupling analyzed (additional alanine substitutions .sup.2G.sub.int Effect of the coupling on the shown in parentheses) (kJ/mol) Activation Energy F145A:S211A 1.3 Destabilizing L202A:S211A 2.4 Destabilizing S211A:T221A 0.0 None F145A:L202A (S211A) 2.9 Destabilizing F145A:T221A (S211A) 2.0 Destabilizing L202A:T221A (S211A) 1.5 Destabilizing F145A:L202A (S211A/T221A) 1.2 Destabilizing F145A:T221A (L202A/S211A) 0.3 Negligible L202A:T221A (F145A/S211A) 0.2 Negligible
(88) TABLE-US-00008 TABLE S5 Ternary Free Energies of Coupling between F145A, L202A, and T221A Coupling analyzed (additional alanine substitutions .sup.3G.sub.int shown in parentheses) (kJ/mol) Effect of the coupling on the stability of the EL-Tyr-tRNA Complex F145A:S211A:T221A (S211A) 0.8 Destabilizing Effect of the coupling on the stability of the E[L-Tyr-tRNA].sup. Complex F145A:S211A:T221A (S211A) 1.6 Stabilizing Effect of the coupling on the Activation Energy F145A:S211A:T221A (S211A) 1.7 Stabilizing
(89) The invention illustratively disclosed herein suitably may explicitly be practiced in the absence of any element which is not specifically disclosed herein. While various embodiments of the present invention have been described in detail, it is apparent that various modifications and alterations of those embodiments will occur to and be readily apparent those skilled in the art. However, it is to be expressly understood that such modifications and alterations are within the scope and spirit of the present invention, as set forth in the appended claims. Further, the invention(s) described herein is capable of other embodiments and of being practiced or of being carried out in various other related ways. In addition, it is to be understood that the phraseology and terminology used herein is for the purpose of description and should not be regarded as limiting. The use of including, comprising, or having and variations thereof herein is meant to encompass the items listed thereafter and equivalents thereof as well as additional items while only the terms consisting of and consisting only of are to be construed in the limitative sense.