BIOCOMPATIBLE MICROELECTRODE

Abstract

A biocompatible microelectrode for in vivo and/or in vitro instant monitoring of the neurotransmitter release and reuptake for brain diseases and behavior disorders, which has a recording surface located on the end portion that contacts the neurotransmitter and a conductive circuit which is positioned on the holding portion which enables holding the biocompatible microelectrode during application, and which transfers the measurement data taken from the recording surface to sensors/measurement device. Selective and precise in vivo and in vitro measurement of the neurotransmitter is enabled by coating the isolated recording surface of the biocompatible microelectrode with a biocompatible surface coating.

Claims

1. A biocompatible microelectrode used for instant in vitro and in vivo imaging of the neurotransmitter release and reuptake in brain diseases and behavior disorders, comprising; a recording surface obtaining the measurement data and located on an end portion that contacts the neurotransmitter, a conductive circuit positioned on a holding portion that enables holding the biocompatible microelectrode during application, transferring data received from a recording surface to sensors/measurement device for measurement, the biocompatible microelectrode characterized by comprising; a biocompatible surface coating coated on the isolated recording surface to enable selective and precise in vivo and in vitro measurement of the neurotransmitter.

2. The biocompatible microelectrode according to claim 1, comprising an earthing inlet positioned between the end portion and the holding portion, on which the earthing is connected to prevent noise during measurement.

3. The biocompatible microelectrode according to claim 1, wherein the recording surface isolation material includes positive and negative photoresist.

4. The biocompatible microelectrode according to claim 1, wherein the biocompatible surface coating is AgNPs/graphene oxide, 2-aminobenzoimidasole or 2-aminobenzothiasole.

5. The biocompatible microelectrode according to claim 1, comprising a silicone base which constitutes the base of the holding portion, enabling holding the electrode in hand during application and protecting it against falling and breaking.

6. The biocompatible microelectrode according to claim 1, comprising a ceramic base which provides surface adhesion and isolation, and forming the base of the end portion.

7. The biocompatible microelectrode according to claim 1, wherein the recording surface is made of platinum.

8. The biocompatible microelectrode according to claim 1, wherein the conductive circuit is made of copper.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0014] FIG. 1 is top view of the invented biocompatible microelectrode.

[0015] FIG. 2 is detailed view of the end portion of the invented biocompatible microelectrode.

[0016] FIG. 3 is detailed view of the holding portion of the invented biocompatible microelectrode.

[0017] FIG. 4 shows detailed views of the different types of the invented biocompatible microelectrode.

[0018] The drawings are not necessarily scaled and the details that are not essential to understand the present invention may have been omitted. Apart from that, the elements which are at least substantially identical or have at least substantially identical functions are shown with the same reference number.

REFERENCE NUMBERS

[0019] 1. Biocompatible microelectrode [0020] A. End portion [0021] 10. Ceramic base [0022] 20. Recording surface [0023] B. Holding portion [0024] 30. Silicone base [0025] 40. Conductor circuit [0026] C. Earthing inlet

DETAILED DESCRIPTION OF THE INVENTION

[0027] The preferred embodiments of the invention are described herein with certain embodiments only to facilitate understanding the invention and without having any restrictive effect.

[0028] The invention is related with a biocompatible microelectrode (1) for in vivo and/or in vitro instant monitoring of the neurotransmitter release and reuptake for brain diseases and behavior disorders, which comprises a recording surface (20) located on the end portion (A) that contacts the neurotransmitter and a conductive circuit (40) which is positioned on the holding portion (B) which enables holding the biocompatible microelectrode (1) during application, and which transfers the measurement data taken from the recording surface (20) to sensors/measurement device. Selective and precise in vivo and in vitro measurement of the neurotransmitter is enabled by coating a biocompatible microelectrode (1) surface coating on the isolated recording surface (20) of the biocompatible microelectrode.

[0029] FIG. 1 is the top view of the invented biocompatible microelectrode. According to this figure, the invention substantially comprises an end portion (A) which obtains the measurement results, and a holding portion (B) which enables holding the device during application. These two main elements are connected to each other with an earthing inlet (C). Thanks to positioning the earthing inlet (C) of the earthing connection on the electrode, in the middle of the end portion (A) and the holding portion (B), the level of impacting the measurement results by preventing the noise realized at the background during measurement has been reduced.

[0030] FIG. 2 is the detail view (A) of the end portion (A) of the invented biocompatible microelectrode. Accordingly, the ceramic base (10) of the end portion (A) constitutes the main structure. The preferably platinum recording surface (20) which enables realizing the recording operation is located on the ceramic base (10) that enables adhesion to the surface and enabling isolation. The ceramic base (10) that constitutes the end portion (A) base has a biocompatible and non-toxic structure.

[0031] FIG. 3 is the detail view of the holding portion (B) of the invented biocompatible microelectrode (1). The holding portion (B) comprises a silicon base (30) in a form to prevent falling and breaking of the electrode during application by facilitating its being held in hand. The copper conductor circuit (40) positioned on the silicon base (30) which constitutes the base of the holding portion (B) enables moving the measurement results received from the end point (A) to the device. Copper conductive circuit (40) is attached by printing on a wide base made of silicate and plastic.

[0032] The device detects the currents at nA level, and sends them to the analysis software of the computer wirelessly after the reinforcement and digitization operations. The device is portable the dimensions of 0.6 cm3.7 cm2.4 cm and 7.1 gr weight, and briefly realizes the following operations.

[0033] After connecting to the biocompatible microelectrode (1), the current is created in proportion to the neurotransmitter concentration in the environment of the biocompatible microelectrode (1) with 0.7 V activation energy received from the device. This current at nanoampere levels is sent to the device's intensifier. The read current values are filtered and digitized, and then transferred through the microcontroller to the analysis interface prepared with computer software by Bluetooth. It is analyzed with analysis algorithms written on this interface.

[0034] For surface coating, AgNPs/graphene oxide (silver nanoparticle added graphene oxide) is used on the recording surface (20). For selectivity, barriers were created with 2-aminobenzoimidasole, 2-aminobenzothiasole derivatives according to the type of neurotransmitter, and biocompatible, selective, precise electrodes were obtained. This surface coating can maintain its measurement stability after several uses both in vivo and in vitro thanks to its surface coating.

[0035] Biocompatible positive photoresist and negative photoresist with high breakdown voltages and compatible dielectric coefficients suitable for high resolution fabrication were used for the isolation of the microelectrodes as the isolation layer/substance on the recording surface (20).

[0036] Different forms of biocompatible microelectrodes (1) in FIG. 4 are the illustrations of different embodiments of the invention. Many embodiments may be created according to the foregoing descriptions in many different forms and combinations.