1,1,1-trifluoro-3-hydroxypropan-2-yl carbamate derivatives and 1,1,1-trifluoro-4-hydroxybutan-2-yl carbamate derivatives as MAGL inhibitors

Abstract

The present invention provides, in part, compounds of Formula I: ##STR00001##
and pharmaceutically acceptable salts thereof; processes for the preparation of; intermediates used in the preparation of; and compositions containing such compounds or salts, and their uses for treating MAGL-mediated diseases and disorders including, e.g., pain, an inflammatory disorder, traumatic brain injury, depression, anxiety, Alzheimer's disease, a metabolic disorder, stroke, or cancer.

Claims

1. A compound of Formula I-a or I-a1: ##STR00193## or a pharmaceutically acceptable salt thereof, wherein: the moiety of N(R.sup.1)(R.sup.2) is a moiety of Formula a-13: ##STR00194## t1 is 0, 1, 2, or 3; each of R.sup.5 and R.sup.6 is independently H or C.sub.1-4 alkyl; R.sup.7 is H, C.sub.1-6 alkyl, C.sub.3-7 cycloalkyl, or R.sup.10, wherein the C.sub.1-6 alkyl of R.sup.7 is optionally substituted with one or more substituents each independently selected from the group consisting of OH, halogen, C.sub.1-4 alkoxy, C.sub.1-4 haloalkoxy, and C.sub.3-6 cycloalkyl, and wherein the C.sub.3-7 cycloalkyl of R.sup.7 is optionally substituted with one or more substituents each independently selected from the group consisting of OH, halogen, C.sub.1-4 alkyl, C.sub.1-4 haloalkyl, C.sub.1-4 alkoxy, and C.sub.1-4 haloalkoxy; R.sup.8 is L.sup.1-R.sup.11, -L.sup.2-R.sup.12, -L.sup.3-R.sup.13, -L.sup.4-R.sup.14, C(R.sup.15)(Cy.sup.1)(Cy.sup.2), C(R.sup.15)(Cy.sup.1)[NR.sup.23S(O).sub.2-Cy.sup.2], or -L.sup.5-N(-L.sup.6-Cy.sup.3)(-L.sup.7-Cy.sup.4); each R.sup.9 is independently OH, oxo, halogen, optionally substituted C.sub.1-4 alkyl, optionally substituted C.sub.1-4 alkoxy, or optionally substituted C.sub.3-6 cycloalkyl; R.sup.10 is P(O)(OR.sup.81)(OR.sup.82) or S(O).sub.2OR.sup.90; each of L.sup.1, L.sup.2, L.sup.3, and L.sup.4 is independently absent, (CR.sup.21R.sup.22).sub.m, NR.sup.23, O, C(O), S(O).sub.2, S(O).sub.2(CR.sup.21R.sup.22).sub.n, C(O)(CR.sup.21R.sup.22).sub.n, S(O).sub.2NR.sup.23, C(O)NR.sup.23, (CR.sup.21R.sup.22).sub.f1NR.sup.23(CR.sup.21R.sup.22).sub.f2, (CR.sup.21R.sup.22).sub.f1O(CR.sup.21R.sup.22).sub.f2, C(O)NR.sup.23(CR.sup.21R.sup.22).sub.p, or S(O).sub.2NR.sup.23(CR.sup.21R.sup.22).sub.p; L.sup.5 is absent or (CR.sup.21R.sup.22); L.sup.6 is absent or (CR.sup.21R.sup.22); L.sup.7 is absent, (CR.sup.21R.sup.22), or S(O).sub.2; R.sup.11 is 5- to 10-membered heteroaryl optionally substituted with one or more independently selected R.sup.31; R.sup.12 is 4- to 14-membered heterocycloalkyl optionally substituted with one or more independently selected R.sup.32; R.sup.13 is C.sub.6-10 aryl optionally substituted with one or more independently selected R.sup.33; R.sup.14 is C.sub.3-14 cycloalkyl optionally substituted with one or more independently selected R.sup.34; R.sup.15 is H, OH, halogen, C.sub.1-4 alkoxy, C.sub.1-4 alkyl, or cyclopropyl; each of R.sup.21 and R.sup.22 is independently H, OH, halogen, C.sub.1-3 alkyl, or cyclopropyl, wherein the C.sub.1-3 alkyl is optionally substituted with one or more substituents each independently selected from the group consisting of OH, halogen, C.sub.1-3 alkoxy, C.sub.1-3 haloalkoxy, and cyclopropyl; R.sup.23 is H, C.sub.1-4 alkyl, or cyclopropyl; each of R.sup.31, R.sup.32, R.sup.33, and R.sup.34 is independently selected from the group consisting of halogen, N(R.sup.a)(R.sup.b), N(R.sup.c)(C(O)R.sup.d), N(R.sup.c)(S(O).sub.2R.sup.d), C(O)N(R.sup.a)(R.sup.b), C(O)R.sup.d, C(O)OR.sup.d, OC(O)R.sup.d, N(R.sup.c)(S(O).sub.2R.sup.d), S(O).sub.2N(R.sup.a)(R.sup.b), SR.sup.d, S(O).sub.2R.sup.d, OR.sup.d, OR.sup.35, CN, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.3-10 cycloalkyl, 4- to 10-membered heterocycloalkyl, C.sub.6-10 aryl, 5- to 10-membered heteroaryl, (C.sub.3-10 cycloalkyl)-C.sub.1-4 alkyl-, (4- to 10-membered heterocycloalkyl)-C.sub.14 alkyl-, (C.sub.6-10 aryl)-C.sub.1-4 alkyl-, and (5- to 10-membered heteroaryl)-C.sub.1-4 alkyl-, wherein each of the C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.3-10 cycloalkyl, 4- to 10-membered heterocycloalkyl, C.sub.6-10 aryl, 5- to 10-membered heteroaryl, (C.sub.3-10 cycloalkyl)-C.sub.1-4 alkyl-, (4- to 10-membered heterocycloalkyl)-C.sub.1-4 alkyl-, (C.sub.6-10 aryl)-C.sub.1-4 alkyl-, and (5- to 10-membered heteroaryl)-C.sub.1-4 alkyl- is optionally substituted with one or more independently selected R.sup.36; and wherein each of the C.sub.1-6 alkyl, C.sub.3-10 cycloalkyl, 4- to 10-membered heterocycloalkyl, (C.sub.3-10 cycloalkyl)-C.sub.1-4 alkyl-, (4- to 10-membered heterocycloalkyl)-C.sub.1-4 alkyl-, (C.sub.6-10 aryl)-C.sub.1-4 alkyl-, and (5- to 10-membered heteroaryl)-C.sub.1-4 alkyl- is further optionally substituted one or more oxo; each R.sup.35 is independently selected from the group consisting of H, C.sub.1-6 alkyl, C.sub.3-10 cycloalkyl, 4- to 10-membered heterocycloalkyl, C.sub.6-10 aryl, 5- to 10-membered heteroaryl, (C.sub.3-10 cycloalkyl)-C.sub.1-4 alkyl-, (4- to 10-membered heterocycloalkyl)-C.sub.1-4 alkyl-, (C.sub.6-10 aryl)-C.sub.1-4 alkyl-, and (5- to 10-membered heteroaryl)-C.sub.1-4 alkyl-, wherein each of the C.sub.1-6 alkyl, C.sub.3-10 cycloalkyl, 4- to 10-membered heterocycloalkyl, C.sub.6-10 aryl, 5- to 10-membered heteroaryl, (C.sub.3-10 cycloalkyl)-C.sub.1-4 alkyl-, (4- to 10-membered heterocycloalkyl)-C.sub.1-4 alkyl-, (C.sub.6-10 aryl)-C.sub.1-4 alkyl-, and (5- to 10-membered heteroaryl)-C.sub.1-4 alkyl- is optionally substituted with one or more substituents independently selected from the group consisting of halogen, CN, C(O)C.sub.1-4 alkyl, C(O)OH, C(O)OC.sub.1-4 alkyl, C(O)NHC.sub.1-4 alkyl, C(O)N(C.sub.1-4 alkyl).sub.2, oxo, OH, OC(O)C.sub.1-4 alkyl, OC(O)OC.sub.1-4 alkyl, NH2, NH(C.sub.1-4 alkyl), N(C.sub.1-4 alkyl).sub.2, NHC(O)C.sub.1-4 alkyl, NHC(O)OC.sub.1-4 alkyl, NHC(O)NHC.sub.1-4 alkyl, and C.sub.1-4 alkoxy; each R.sup.36 is independently selected from the group consisting of halogen, OH, NO.sub.2, CN, SF.sub.5, C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 haloalkoxy, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.3-7 cycloalkyl, a 4- to 10-membered heterocycloalkyl, N(R.sup.a)(R.sup.b), N(R.sup.c)(C(O)R.sup.d), C(O)N(R.sup.a)(R.sup.b), C(O)R.sup.d, C(O)OR.sup.d, OC(O)R.sup.d, N(R.sup.c)(S(O).sub.2R.sup.d), S(O).sub.2N(R.sup.a)(R.sup.b), SR.sup.d, S(O).sub.2R.sup.d, and OR.sup.d, wherein each of the C.sub.1-6 alkyl, C.sub.3-7 cycloalkyl, and heterocycloalkyl is optionally substituted with one or more substituents each independently selected from the group consisting of halogen, CN, OH, C.sub.1-4 alkyl, C.sub.1-4 alkoxy, C.sub.1-4 haloalkyl, C.sub.1-4 haloalkoxy, C.sub.3-6 cycloalkyl, N(R.sup.a)(R.sup.b), N(R.sup.c)(C(O)R.sup.d), C(O)OR.sup.d, C(O)H, C(O)R.sup.d, C(O)N(R.sup.a)(R.sup.b), N(R.sup.c)(S(O).sub.2R.sup.d), S(O).sub.2N(R.sup.a)(R.sup.b), SR.sup.d, S(O).sub.2R.sup.d, and OR.sup.d; each of R.sup.81, R.sup.82, and R.sup.90 is independently selected from the group consisting of H, C.sub.1-6 alkyl, C.sub.3-7 cycloalkyl, and (C.sub.3-7 cycloalkyl)-C.sub.1-4 alkyl-, wherein each of the C.sub.1-6 alkyl, C.sub.3-7 cycloalkyl, and (C.sub.3-7 cycloalkyl)-C.sub.1-4 alkyl- is optionally substituted with one or more substituents each independently selected from the group consisting of halogen, CN, OH, oxo, NH.sub.2, NH(C.sub.1-4 alkyl), N(C.sub.1-4 alkyl).sub.2, C.sub.1-4 alkyl, C.sub.1-4 alkoxy, C.sub.1-4 haloalkyl, C.sub.1-4 haloalkoxy, C.sub.3-6 cycloalkyl; or OR.sup.81 and OR.sup.82, together with the P(O) to which they are attached, form 4- to 10-membered heterocycloalkyl that is further optionally substituted with one or more substituents each independently selected from the group consisting of halogen, CN, OH, oxo, NH.sub.2, NH(C.sub.1-4 alkyl), N(C.sub.1-4 alkyl).sub.2, C.sub.1-4 alkyl, C.sub.1-4 alkoxy, C.sub.1-4 haloalkyl, C.sub.1-4 haloalkoxy, and C.sub.3-6 cycloalkyl; each of Cy.sup.1, Cy.sup.2, Cy.sup.3, and Cy.sup.4 is independently selected from the group consisting of R.sup.11, R.sup.12, R.sup.13, and R.sup.14; each R.sup.a is independently H, C.sub.1-4 alkyl, C.sub.1-4 haloalkyl, C.sub.3-7 cycloalkyl, or (C.sub.3-7 cycloalkyl)-C.sub.1-4 alkyl-; each R.sup.b is independently H or selected from the group consisting of C.sub.1-4 alkyl, C.sub.1-4 haloalkyl, C.sub.3-7 cycloalkyl, a 4- to 10-membered heterocycloalkyl, C.sub.6-10 aryl, a 5- to 10-membered heteroaryl, (C.sub.3-7 cycloalkyl)-C.sub.1-4 alkyl-, (4- to 10-membered heterocycloalkyl)-C.sub.1-4 alkyl-, (C.sub.6-10 aryl)-C.sub.1-4 alkyl-, and (5- to 10-membered heteroaryl)-C.sub.1-4 alkyl-, wherein each of the selections from the group is optionally substituted with one or more substituents each independently selected from the group consisting of OH, CN, C.sub.1-4 alkyl, C.sub.3-7 cycloalkyl, C.sub.1-4 hydroxylalkyl, SC.sub.1-4 alkyl, C(O)H, C(O)C.sub.1-4 alkyl, C(O)OC.sub.1-4 alkyl, C(O)NH.sub.2, C(O)N(C.sub.1-4 alkyl).sub.2, C.sub.1-4 haloalkyl, C.sub.1-4 alkoxy, and C.sub.1-4 haloalkoxy; or R.sup.a and R.sup.b, together with the N atom to which they are attached, form a 4- to 10-membered heterocycloalkyl or a 5- to 10-membered heteroaryl, each optionally substituted with one or more substituents each independently selected from the group consisting of halogen, OH, oxo, C(O)H, C(O)OH, C(O)C.sub.1-4 alkyl, C(O)NH.sub.2, C(O)N(C.sub.1-4 alkyl).sub.2, CN, C.sub.1-4 alkyl, C.sub.3-6 cycloalkyl, (C.sub.3-6 cycloalkyl)-C.sub.1-2 alkyl-, C.sub.1-4 alkoxy, C.sub.1-4 hydroxylalkyl, C.sub.1-4 haloalkyl, and C.sub.1-4 haloalkoxy; each R.sup.c is independently selected from the group consisting of H, C.sub.1-4 alkyl, C.sub.3-7 cycloalkyl, and (C.sub.3-7 cycloalkyl)-C.sub.1-4 alkyl-; each R.sup.d is independently selected from the group consisting of C.sub.1-6 alkyl, C.sub.3-7 cycloalkyl, a 4- to 14-membered heterocycloalkyl, C.sub.6-10 aryl, a 5- to 10-membered heteroaryl, (C.sub.3-7 cycloalkyl)-C.sub.1-4 alkyl-, (4- to 10-membered heterocycloalkyl)-C.sub.1-4 alkyl-, (C.sub.6-10 aryl)-C.sub.1-4 alkyl-, and (5- to 10-membered heteroaryl)-C.sub.1-4 alkyl-, wherein each of the selections from the group is optionally substituted with one or more substituents each independently selected from the group consisting of halogen, CF.sub.3, CN, OH, oxo, SC.sub.1-4 alkyl, C.sub.1-4 alkyl, C.sub.1-4 haloalkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.3-7 cycloalkyl, C.sub.1-4 alkoxy, and C.sub.1-4 haloalkoxy; each of f1 and f2 is independently 0, 1, or 2, provided that the sum of f1 and f2 is 1, 2, or 3; m is 1, 2, or 3; n is 1, 2, or 3; and p is 1, or 2.

2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein the compound is a compound of I-a1.

3. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein each of R.sup.5 and R.sup.6 is independently H or methyl.

4. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein each of R.sup.5 and R.sup.6 is H.

5. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R.sup.7 is H or R.sup.10; and R.sup.10 is P(O)(OR.sup.81)(OR.sup.82).

6. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R.sup.7 is H.

7. The compound of claim 4, or a pharmaceutically acceptable salt thereof, wherein: R.sup.8 is -L.sup.1-R.sup.11, -L.sup.2-R.sup.12, -L.sup.3-R.sup.13, or -L.sup.4-R.sup.14; each of L.sup.1, L.sup.2, L.sup.3, and L.sup.4 is independently absent, (CR.sup.21R.sup.22), C(O), S(O).sub.2, S(O).sub.2NR.sup.23, S(O).sub.2(CR.sup.21R.sup.22), S(O).sub.2NR.sup.23(CR.sup.21R.sup.22), or S(O).sub.2(CR.sup.21R.sup.22).sub.2; each of R.sup.21 and R.sup.22 is independently H, C.sub.1-3 alkyl, or cyclopropyl; R.sup.11 is 5- to 6-membered heteroaryl optionally substituted with one or more independently selected R.sup.31; R.sup.12 is 5- to 6-membered heterocycloalkyl optionally substituted with one or more independently selected R.sup.31; R.sup.13 is phenyl optionally substituted with one or more independently selected R.sup.33; and R.sup.14 is C.sub.3-8 cycloalkyl optionally substituted with one or more independently selected R.sup.34.

8. The compound of claim 7, or a pharmaceutically acceptable salt thereof, wherein R.sup.8 is -L.sup.1-R.sup.11 or -L.sup.3-R.sup.13.

9. The compound of claim 7, or a pharmaceutically acceptable salt thereof, wherein R.sup.8 is R.sup.11 or R.sup.13.

10. The compound of claim 7, or a pharmaceutically acceptable salt thereof, wherein R.sup.8 is R.sup.11.

11. A compound selected from the group consisting of: (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(5-methoxypyridin-2-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate; (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate; rel-(2S,3R)-1,1,1,4,4,4-hexafluoro-3-hydroxybutan-2-yl (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate; (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-641-(pyridin-2-ylmethyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate; 1,1,1,3,3-pentafluoro-4-hydroxybutan-2-yl (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate; (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(6-methoxypyridin-3-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate; (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(4-ethynylphenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate; and (2S)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(4-ethynylphenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate, or a pharmaceutically acceptable salt thereof.

12. A compound of claim 1 that is (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(5-methoxypyridin-2-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate, or a pharmaceutically acceptable salt thereof.

13. A compound of claim 1 that is (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate, or a pharmaceutically acceptable salt thereof.

14. A pharmaceutical composition comprising a compound of claim 1 or pharmaceutically acceptable salt, and a pharmaceutically acceptable carrier.

15. A pharmaceutical composition comprising a compound of claim 1 or pharmaceutically acceptable salt, and a pharmaceutically acceptable carrier.

16. A method for treating a MAGL-mediated disease or disorder in a mammal, which method comprises administering to said mammal a therapeutically effective amount of a compound of claim 1 or pharmaceutically acceptable salt thereof, wherein the disease or disorder is selected from the group consisting of nausea; neuropathy; a neurodegenerative disorder; multiple sclerosis; Parkinson's disease; tremor; dyskinesia; dystonia; spasticity; Tourette's syndrome; an inflammatory disorder; neuroinflammation; inflammation in the central nervous system; and pain.

17. A method for inhibiting MAGL comprising contacting the MAGL with a compound of claim 1 or pharmaceutically acceptable salt thereof.

18. A pharmaceutical composition comprising a compound of claim 11 or pharmaceutically acceptable salt, and a pharmaceutically acceptable carrier.

Description

DETAILED DESCRIPTION OF THE INVENTION

(1) Compounds of the invention, including salts of the compounds, can be prepared using known organic synthesis techniques and can be synthesized according to any of numerous possible synthetic routes. The reactions for preparing compounds of the invention can be carried out in suitable solvents, which can be readily selected by one of skill in the art of organic synthesis. Suitable solvents can be substantially non-reactive with the starting materials (reactants), the intermediates, or products at the temperatures at which the reactions are carried out, e.g., temperatures that can range from the solvent's freezing temperature to the solvent's boiling temperature. A given reaction can be carried out in one solvent or a mixture of more than one solvent. Depending on the particular reaction step, suitable solvents for a particular reaction step can be selected by the skilled artisan.

(2) Preparation of compounds of the invention can involve the protection and deprotection of various chemical groups. The need for protection and deprotection, and the selection of appropriate protecting groups, can be readily determined by one skilled in the art. The chemistry of protecting groups can be found, for example, in T. W. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 3.sup.rd Ed., Wiley & Sons, Inc., New York (1999), which is incorporated herein by reference in its entirety.

(3) Reactions can be monitored according to any suitable method known in the art. For example, product formation can be monitored by spectroscopic means, such as nuclear magnetic resonance spectroscopy (e.g., .sup.1H or .sup.13C), infrared spectroscopy, spectrophotometry (e.g., UV-visible), mass spectrometry, or by chromatographic methods such as high-performance liquid chromatography (HPLC) or thin layer chromatography (TLC).

(4) Compounds of Formula I and intermediates thereof may be prepared according to the following reaction schemes and accompanying discussion. Unless otherwise indicated, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, r and structural Formula I (including I-a) in the reaction schemes and discussion that follow are as defined above. In general, the compounds of this invention may be made by processes which include processes analogous to those known in the chemical arts, particularly in light of the description contained herein. Certain processes for the manufacture of the compounds of this invention and intermediates thereof are provided as further features of the invention and are illustrated by the following reaction schemes. Other processes are described in the experimental section. The schemes and examples provided herein (including the corresponding description) are for illustration only, and not intended to limit the scope of the present invention.

(5) Scheme 1 refers to the synthesis of compounds of Formula I. Referring to Scheme 1, a compound of Formula 1-3 [wherein Pg.sup.1 is an alcohol protecting group such as tert-butyldimethyl silyl (TBDMS) or p-methoxbenzyl] can be prepared by reacting an amine of Formula 1-1 with a compound of Formula 1-2 using standard methods of carbamate formation well known to those skilled in the art [for example, in the presence of phosgene, triphosgene, or a suitably activated carbonate reagent such as bis(pentafluorophenyl)carbonate or N,N-disuccinimidyl carbonate]. Amines of Formula 1-1 may be obtained commercially, synthesized by methods described herein, or made by other methods well known to those skilled in the art. Carbamate formation may be accomplished in the presence of a base (such as triethylamine or hunigs base). A compound of Formula 1-4 may be obtained by deprotecting the compounds of Formula 1-3, using appropriate conditions depending on the selection of the Pg.sup.1 group. For example, where Pg.sup.1 is TBDMS, treatment with an acid such as trifluoroacetic acid in aprotic solvent such as dichloromethane may be employed. The compound of Formula 1-4 (which is a compound of Formula I wherein R.sup.7 is H) may optionally be converted to a compound of Formula I wherein R.sup.7 is other than H. For example, an alkylation reaction of the compound of Formula 1-4 with a halide compound (alkyl halide or cycloalkyl halide) can provide a compound of Formula I wherein R.sup.7 is C.sub.1-6 alkyl, C.sub.3-7 cycloalkyl. As another example, reaction of the alcohol of Formula 1-4 with diphosphoryl tetrachloride in a suitable solvent such as acetonitrile affords compounds of Formula I where R.sup.7 is P(O)(OH).sub.2 or a salt thereof. For yet another example, reaction of the alcohol of Formula 1-4 with a sulfating agent [e.g. SO.sub.3, sulfamic acid H.sub.2NS(O).sub.2(OH), chlorosulfonic acid HOS(O).sub.2(Cl)] under suitable conditions can afford a compound of Formula I wherein R.sup.7 is S(O).sub.2(OH) or a salt thereof.

(6) ##STR00030##

(7) Scheme 2 refers to synthesis of compounds of Formula I-a. An amine of Formula 1-1 may be reacted with a compound of Formula 2-2 [where Pg.sup.1 is a suitable alcohol protecting group, such as TBDMS or p-methoxybenzyl], using methods analogous to those described in Scheme 1, to form a carbamate of Formula 2-3. The compound of Formula 2-3 may be deprotected using appropriate conditions depending of the selection of Pg.sup.1 to give a compound of Formula 2-4. Similar to the discussions in Scheme 1, the compound of Formula 2-4 (which is a compound of Formula I-a wherein R.sup.7 is H) may optionally be converted to a compound of Formula I-a wherein R.sup.7 is other than H.

(8) ##STR00031##

(9) Scheme 3 refers to the preparation of compounds of Formula 3-4 [wherein Pg.sup.1 is an alcohol protecting group such TBDMS or p-methoxbenzyl], which can be used as a compound of Formula 1-2 in Scheme 1 [wherein r is 1; and both R.sup.5 and R.sup.6 are H]. Referring to Scheme 3, a compound of Formula 3-3 may be prepared by treatment of compound 3-1 with a base (such as n-butyllithium) followed by addition to formaldehyde 3-2 (or its equivalent such as paraformaldehyde) in the presence of a reducing agent such as sodium borohydride. Protection of the alcohol moiety in the compound of Formula 3-3 may be achieved by methods known to those skilled in the art. For example, where the Pg.sup.1 is TBDMS, the protection can be achieved by treatment of the compound of Formula 3-3 with an activated silyl reagent [such as tert-butyl(dimethyl)silyl chloride] in the presence of a base (such as 1H-imidazole) in a suitable non-protic solvent (such as THF or DMF) at a suitable temperature (e.g., ambient temperature).

(10) ##STR00032##

(11) Scheme 4 refers to a synthesis of compounds of Formula 4-3 [wherein Pg.sup.2 is an alcohol protecting group such p-methoxbenzyl], which can be used as a compound of Formula 1-2 in Scheme 1 [wherein r is 0]. Referring to Scheme 4, reaction of an epoxide of Formula 4-1 with an alcohol of Formula 4-2, in the presence of a base [e.g., NaN(TMS).sub.2) in a in non-protic solvent (e.g., THF or DMF), affords a compound of Formula 4-3.

(12) ##STR00033##

(13) Scheme 4A refers to a synthesis of a compound of Formula 4A-5 or a salt thereof [i.e., a compound of Formula I-a or salt thereof, wherein R.sup.7 is P(O)(OH).sub.2]. Referring to Scheme 4A, reaction of an epoxide of Formula 4A-1 with a phosphorus compound of Formula 4A-2 [wherein each of Pg.sup.2A is a hydroxyl protecting group such as benzyl], optionally in the presence of a base [e.g., NaN(TMS).sub.2] in a in non-protic solvent (e.g., THF or DMF), affords a compound of Formula 4A-3. Similar to the carbamate formation reaction described in Schemes 1 and 2, reaction of the compound of Formula 4A-3 and an amine of Formula 1-1 affords a compound of Formula 4A-4. Depending on the choice of the Pg.sup.2A groups, removal of the protecting Pg.sup.2A groups under suitable conditions will afford a compound of Formula 4A-5 or a salt thereof.

(14) ##STR00034##

(15) Scheme 5 refers to the preparation of amines of Formula 5-8 (wherein R.sup.31 is aryl or heteroaryl that are optionally substituted), which can be used as a specific type of amine of Formula 1-1 for the preparation of compounds of Formula I or I-a in Schemes 1 and 2. The Weinreb amide of Formula 5-2 [where Pg.sup.3 is an amine protecting group such as tert-butoxycarbonyl (BOC)] can be prepared by coupling N-methoxymethanamine with a carboxylic acid of Formula 5-1 using a suitable coupling agent [e.g., O-(7-azabenzotriazol-1-yl)-N,N,N,N tetramethyluronium hexafluorophosphate (HATU)]. Addition of a Grignard reagent (e.g., methylmagnesium bromide) to the Weinreb amide of Formula 5-2 results in a ketone of Formula 5-3. Treatment of the ketone of Formula 5-3 with N,N-dimethylformamide dimethyl acetal at elevated temperatures results in an enamine of Formula 5-4. Subsequent treatment with hydrazine (or its equivalent) in a protic solvent such as ethanol affords a pyrazole of Formula 5-5. A compound of Formula 5-7 can be obtained by reacting the pyrazole of Formula 5-5 with a (hetero)aryl boronic acid of Formula 5-6 in the presence of a catalyst (such as copper acetate) and a base (e.g., pyridine) in a suitable solvent (such as dichloromethane). Alternatively, the pyrazole of Formula 5-5 can be transformed into the compound of Formula 5-7 by palladium-catalyzed coupling with a suitable (hetero)aryl halide of Formula 5-9 wherein X is a suitable halide such are Cl, Br or I. Coupling can be achieved by reaction of the pyrazole of Formula 5-5 and (hetero)aryl halide of Formula 5-9 in the presence of a palladium catalyst such as [1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II) [Pd(dppf)Cl.sub.2] together with a base such as potassium acetate at an elevated temperature in a non-protic solvent such as toluene. A compound of Formula 5-8 can be prepared by removal of the protecting group Pg.sup.3. For example, wherein the Pg.sup.3 is tert-butoxycarbonyl (BOC), cleavage can be achieved under acidic conditions by treatment with, for example, trifluoroacetic acid.

(16) ##STR00035##

(17) Scheme 6 refers to a synthesis of a spiromorpholine of Formula 6-6 (wherein Pg.sup.4 is a suitable amine protecting group such as BOC), which can be used as a starting material in Scheme 7. Referring to Scheme 6, reaction of a suitably protected piperidine of Formula 6-1 with nitromethane in the presence of a mild base such as triethylamine affords a compound of Formula 6-2. Reduction of the nitro moiety of the compound of Formula 6-2 to obtain an aminoalcohol of Formula 6-3 can be achieved by using methods such as palladium-catalyzed hydrogenation, for example utilizing 10% palladium on carbon in an alcoholic solvent under an atmosphere of hydrogen. Acetylation of the compound of Formula 6-3 can be achieved by treatment with chloroacetyl chloride in the presence of a suitable base such as potassium carbonate. Ring closure of the chloride compound of Formula 6-4 can be achieved by treatment with a suitable base (e.g., potassium tert-butoxide) in a non-protic solvent (e.g., THF) under reflux conditions to furnish a compound of Formula 6-5. A spiromorpholine compound of Formula 6-6 may be obtained by reduction of the amide functionality in the compound of Formula 6-5 using a suitable reducing agent (e.g. borane-dimethyl sulfide complex in THF).

(18) ##STR00036##

(19) Scheme 7 refers to the synthesis of compounds of Formula 7-4, 7-7, 7-10, or 7-13 from an amine of Formula 6-6. A compound of Formula 7-3 [wherein R.sup.70 can be, for example, R.sup.1, R.sup.12, R.sup.13, or R.sup.14] can be prepared by reacting the amine of Formula 6-6 with an aldehyde of Formula 7-2 using reductive amination conditions well known to those skilled in the art. For example, treatment with titanium(IV) isopropoxide and a reducing agent such as sodium borohydride can be employed. Reaction of an amine of Formula 6-6 with sulfonyl chlorides of Formula 7-5 [wherein R.sup.70 can be, for example, R.sup.11, R.sup.12, R.sup.13, or R.sup.14] in the presence of a suitable base (such as pyridine or sodium bicarbonate) results in a sulfonamide of Formula 7-6. An amine 6-6 can be treated with a suitably activated compound of Formula 7-8 (wherein Lg.sup.1 is a leaving group such as Cl) to give a compound of Formula 7-9 [wherein R.sup.71 can be, for example, R.sup.23; and R.sup.72 can be, for example, R.sup.11, R.sup.12, R.sup.13, R.sup.14, (CR.sup.21R.sup.22)R.sup.11, (CR.sup.21R.sup.22)R.sup.12, (CR.sup.21R.sup.22).sub.pR.sup.13, or (CR.sup.21R.sup.22).sub.pR.sup.14; or R.sup.71 and R.sup.72, together with the N atom to which they are attached, form 4- to 14-membered heterocycloalkyl optionally substituted with R.sup.8 and one or more independently selected R.sup.9]. A compound of Formula 7-12 [wherein R.sup.73 can be, for example, R.sup.11 or R.sup.12] can be prepared by metal-catalyzed coupling of compounds of Formula 6-6 with a compound of Formula 7-11 (wherein X is a halogen atom such as Cl or Br). A compound of Formula 7-3, 7-6, 7-9, or 7-12 can be converted to a compound of Formula 7-4, 7-7, 7-10, or 7-13, respectively, by appropriate deprotection. For example, when Pg.sup.4 is BOC, the deprotection can be achieved by treatment with an acid such as trifluoroacetic acid. A compound of Formula 7-4, 7-7, 7-10, or 7-13 can each be used as starting material [as a specific amine of Formula 1-1] for synthesis of compounds of Formula I (e.g., Formula I-a or I-b) as described in Schemes 1 and 2.

(20) ##STR00037##

(21) Scheme 8 refers to a synthesis of compounds of Formula 8-6 [where each t2a is independently 0 or 1; and R.sup.8A can be, for example, R.sup.11, R.sup.12, R.sup.13, or R.sup.14]. A compound of Formula 8-3 can be prepared by treatment of the aminoalcohol of Formula 8-1 (which can be prepared using the method as described in Scheme 6 for the aminoalcohol of Formula 6-3) with a sulfonyl chloride of Formula 8-2 in the presence of a suitable base (e.g., pyridine). Reaction of the compound of Formula 8-3 with a compound of Formula 8-4 (wherein each X is independently a suitable leaving group such as Br or Cl), in the presence of a base such as potassium carbonate in a polar aprotic solvent such as DMF, results in a compound of Formula 8-5. Removal of the protecting group results in a compound of Formula 8-6, which can be used as starting material [as a specific amine of Formula 1-1] in Schemes 1 and 2 for the preparation of compounds of Formula I (including compounds of Formula I-a or I-b).

(22) ##STR00038##

(23) Scheme 9 refers to a preparation of compounds of Formula 9-3 [where R.sup.8A can be, for example, R.sup.11, R.sup.12, R.sup.13, or R.sup.14]. A compound of formula 9-1 [where Pg.sup.4 is an amine protecting group (e.g., BOC)] can be obtained commercially or be readily synthesized by methods well known to those skilled in the art. A compound of Formula 9-2 can be obtained by reaction of a compound of Formula 9-1 with sulfonyl chlorides of Formula 8-2 in a suitable solvent (e.g., dichloromethane) in the presence of a suitable base (e.g., sodium bicarbonate). Deprotection of compounds of Formula 9-2 using appropriate conditions well known to those skilled in the art provides a compound of Formula 9-3. The compound of Formula 9-3 can be used as starting material [as a specific amine of Formula 1-1] in Schemes 1 and 2 for the preparation of compounds of Formula I (including compounds of Formula I-a or I-b).

(24) ##STR00039##

(25) Additional starting materials and intermediates useful for making the compounds of the present invention can be obtained from chemical vendors such as Sigma-Aldrich or can be made according to methods described in the chemical art.

(26) Those skilled in the art can recognize that in all of the schemes described herein, if there are functional (reactive) groups present on a part of the compound structure such as a substituent group, for example R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, etc., further modification can be made if appropriate and/or desired, using methods well known to those skilled in the art. For example, a CN group can be hydrolyzed to afford an amide group; a carboxylic acid can be converted to an amide; a carboxylic acid can be converted to an ester, which in turn can be reduced to an alcohol, which in turn can be further modified. For another example, an OH group can be converted into a better leaving group such as a methanesulfonate, which in turn is suitable for nucleophilic substitution, such as by a cyanide ion (CN.sup.). For another example, an S can be oxidized to S(O) and/or S(O).sub.2. For yet another example, an unsaturated bond such as CC or CC can be reduced to a saturated bond by hydrogenation. One skilled in the art will recognize further such modifications. Thus, a compound of Formula I having a substituent that contains a functional group can be converted to another compound of Formula I having a different substituent group.

(27) Similarly, those skilled in the art can also recognize that in all of the schemes described herein, if there are functional (reactive) groups present on a substituent group such as R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, etc., these functional groups can be protected/deprotected in the course of the synthetic scheme described here, if appropriate and/or desired. For example, an OH group can be protected by a benzyl, methyl, or acetyl group, which can be deprotected and converted back to the OH group in a later stage of the synthetic process. For another example, an NH.sub.2 group can be protected by a benzyloxycarbonyl (Cbz) or BOC group; conversion back to the NH.sub.2 group can be carried out at a later stage of the synthetic process via deprotection.

(28) As used herein, the term reacting (or reaction or reacted) refers to the bringing together of designated chemical reactants such that a chemical transformation takes place generating a compound different from any initially introduced into the system. Reactions can take place in the presence or absence of solvent.

(29) Compounds of Formula I may exist as stereoisomers, such as atropisomers, racemates, enantiomers, or diastereomers. Conventional techniques for the preparation/isolation of individual enantiomers include chiral synthesis from a suitable optically pure precursor or resolution of the racemate using, for example, chiral high-performance liquid chromatography (HPLC). Alternatively, the racemate (or a racemic precursor) may be reacted with a suitable optically active compound, for example, an alcohol, or, in the case where the compound contains an acidic or basic moiety, an acid or base such as tartaric acid or 1-phenylethylamine. The resulting diastereomeric mixture may be separated by chromatography and/or fractional crystallization and one or both of the diastereoisomers converted to the corresponding pure enantiomer(s) by means well known to one skilled in the art. Chiral compounds of Formula I (and chiral precursors thereof) may be obtained in enantiomerically enriched form using chromatography, typically HPLC, on an asymmetric resin with a mobile phase consisting of a hydrocarbon, typically heptane or hexane, containing from 0% to 50% 2-propanol, typically from 2% to 20%, and from 0% to 5% of an alkylamine, typically 0.1% diethylamine. Concentration of the eluate affords the enriched mixture. Stereoisomeric conglomerates may be separated by conventional techniques known to those skilled in the art. See, e.g., Stereochemistry of Organic Compounds by E. L. Eliel and S. H. Wilen (Wiley, New York, 1994), the disclosure of which is incorporated herein by reference in its entirety. Suitable stereoselective techniques are well known to those of ordinary skill in the art.

(30) Where a compound of Formula I contains an alkenyl or alkenylene (alkylidene) group, geometric cis/trans (or Z/E) isomers are possible. Cis/trans isomers may be separated by conventional techniques well known to those skilled in the art, for example, chromatography and fractional crystallization. Salts of the present invention can be prepared according to methods known to those of skill in the art.

(31) The compounds of Formula I that are basic in nature are capable of forming a wide variety of salts with various inorganic and organic acids. Although such salts must be pharmaceutically acceptable for administration to animals, it is often desirable in practice to initially isolate the compound of the present invention from the reaction mixture as a pharmaceutically unacceptable salt and then simply convert the latter back to the free base compound by treatment with an alkaline reagent and subsequently convert the latter free base to a pharmaceutically acceptable acid addition salt. The acid addition salts of the basic compounds of this invention can be prepared by treating the basic compound with a substantially equivalent amount of the selected mineral or organic acid in an aqueous solvent medium or in a suitable organic solvent, such as methanol or ethanol. Upon evaporation of the solvent, the desired solid salt is obtained. The desired acid salt can also be precipitated from a solution of the free base in an organic solvent by adding an appropriate mineral or organic acid to the solution.

(32) If the inventive compound is a base, the desired pharmaceutically acceptable salt may be prepared by any suitable method available in the art, for example, treatment of the free base with an inorganic acid, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, or with an organic acid, such as acetic acid, maleic acid, succinic acid, mandelic acid, fumaric acid, malonic acid, pyruvic acid, oxalic acid, glycolic acid, salicylic acid, isonicotinic acid, lactic acid, pantothenic acid, bitartric acid, ascorbic acid, 2,5-dihydroxybenzoic acid, gluconic acid, saccharic acid, formic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, and pamoic [i.e., 4,4-methanediylbis(3-hydroxynaphthalene-2-carboxylic acid)] acid, a pyranosidyl acid, such as glucuronic acid or galacturonic acid, an alpha-hydroxy acid, such as citric acid or tartaric acid, an amino acid, such as aspartic acid or glutamic acid, an aromatic acid, such as benzoic acid or cinnamic acid, a sulfonic acid, such as ethanesulfonic acid, or the like.

(33) Those compounds of Formula I that are acidic in nature are capable of forming base salts with various pharmacologically acceptable cations. Examples of such salts include the alkali metal or alkaline earth metal salts, and particularly the sodium and potassium salts. These salts are all prepared by conventional techniques. The chemical bases which are used as reagents to prepare the pharmaceutically acceptable base salts of this invention are those which form non-toxic base salts with the acidic compounds of Formula I. These salts may be prepared by any suitable method, for example, treatment of the free acid with an inorganic or organic base, such as an amine (primary, secondary or tertiary), an alkali metal hydroxide or alkaline earth metal hydroxide, or the like. These salts can also be prepared by treating the corresponding acidic compounds with an aqueous solution containing the desired pharmacologically acceptable cations, and then evaporating the resulting solution to dryness, for example under reduced pressure. Alternatively, they may also be prepared by mixing lower alkanolic solutions of the acidic compounds and the desired alkali metal alkoxide together, and then evaporating the resulting solution to dryness in the same manner as before. In either case, stoichiometric quantities of reagents are, for example, employed in order to ensure completeness of reaction and maximum yields of the desired final product.

(34) Pharmaceutically acceptable salts of compounds of Formula I (including compounds of Formula I-a or I-b) may be prepared by, e.g., one or more of three methods:

(35) (i) by reacting the compound of Formula I with the desired acid or base;

(36) (ii) by removing an acid- or base-labile protecting group from a suitable precursor of the compound of Formula I or by ring-opening a suitable cyclic precursor, for example, a lactone or lactam, using the desired acid or base; or

(37) (iii) by converting one salt of the compound of Formula I to another by reaction with an appropriate acid or base or by means of a suitable ion exchange column.

(38) All three reactions are typically carried out in solution. The resulting salt may precipitate out and be collected by filtration or may be recovered by evaporation of the solvent. The degree of ionization in the resulting salt may vary from completely ionized to almost non-ionized.

(39) Polymorphs can be prepared according to techniques well-known to those skilled in the art, for example, by crystallization.

(40) When any racemate crystallizes, crystals of two different types are possible. The first type is the racemic compound (true racemate) referred to above wherein one homogeneous form of crystal is produced containing both enantiomers in equimolar amounts. The second type is the racemic mixture or conglomerate wherein two forms of crystal are produced in equimolar amounts each comprising a single enantiomer.

(41) While both of the crystal forms present in a racemic mixture may have almost identical physical properties, they may have different physical properties compared to the true racemate. Racemic mixtures may be separated by conventional techniques known to those skilled in the artsee, for example, Stereochemistry of Organic Compounds by E. L. Eliel and S. H. Wilen (Wiley, New York, 1994).

(42) The invention also includes isotopically labeled compounds of Formula I wherein one or more atoms is replaced by an atom having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Isotopically labeled compounds of Formula I (or pharmaceutically acceptable salts thereof or N-oxides thereof) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described herein, using an appropriate isotopically labeled reagent in place of the non-labeled reagent otherwise employed.

(43) Prodrugs in accordance with the invention can, for example, be produced by replacing appropriate functionalities present in the compounds of Formula I with certain moieties known to those skilled in the art as pro-moieties as described, for example, in Design of Prodrugs by H. Bundgaard (Elsevier, 1985).

(44) The compounds of Formula I should be assessed for their biopharmaceutical properties, such as solubility and solution stability (across pH), permeability, etc., in order to select the most appropriate dosage form and route of administration for treatment of the proposed indication.

(45) Compounds of the invention intended for pharmaceutical use may be administered as crystalline or amorphous products. They may be obtained, for example, as solid plugs, powders, or films by methods such as precipitation, crystallization, freeze drying, spray drying, or evaporative drying. Microwave or radio frequency drying may be used for this purpose.

(46) They may be administered alone or in combination with one or more other compounds of the invention or in combination with one or more other drugs (or as any combination thereof). Generally, they will be administered as a formulation in association with one or more pharmaceutically acceptable excipients. The term excipient is used herein to describe any ingredient other than the compound(s) of the invention. The choice of excipient will to a large extent depend on factors such as the particular mode of administration, the effect of the excipient on solubility and stability, and the nature of the dosage form.

(47) Pharmaceutical compositions suitable for the delivery of compounds of the present invention (or pharmaceutically acceptable salts thereof) and methods for their preparation will be readily apparent to those skilled in the art. Such compositions and methods for their preparation may be found, for example, in Remington's Pharmaceutical Sciences, 19th Edition (Mack Publishing Company, 1995).

(48) The compounds of the invention (including pharmaceutically acceptable salts thereof) may be administered orally. Oral administration may involve swallowing, so that the compound enters the gastrointestinal tract, and/or buccal, lingual, or sublingual administration by which the compound enters the bloodstream directly from the mouth.

(49) Formulations suitable for oral administration include solid, semi-solid and liquid systems such as tablets; soft or hard capsules containing multi- or nano-particulates, liquids, or powders; lozenges (including liquid-filled); chews; gels; fast-dispersing dosage forms; films; ovules; sprays; and buccal/mucoadhesive patches.

(50) Liquid formulations include suspensions, solutions, syrups and elixirs. Such formulations may be employed as fillers in soft or hard capsules (made, for example, from gelatin or hydroxypropyl methyl cellulose) and typically comprise a carrier, for example, water, ethanol, polyethylene glycol, propylene glycol, methyl cellulose, or a suitable oil, and one or more emulsifying agents and/or suspending agents. Liquid formulations may also be prepared by the reconstitution of a solid, for example, from a sachet.

(51) The compounds of the invention may also be used in fast-dissolving, fast-disintegrating dosage forms such as those described by Liang and Chen, Expert Opinion in Therapeutic Patents 2001, 11, 981-986.

(52) For tablet dosage forms, depending on dose, the drug may make up from 1 weight % to 80 weight % of the dosage form, more typically from 5 weight % to 60 weight % of the dosage form. In addition to the drug, tablets generally contain a disintegrant. Examples of disintegrants include sodium starch glycolate, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, croscarmellose sodium, crospovidone, polyvinylpyrrolidone, methyl cellulose, microcrystalline cellulose, lower alkyl-substituted hydroxypropyl cellulose, starch, pregelatinized starch and sodium alginate. Generally, the disintegrant will comprise from 1 weight % to 25 weight %, for example, from 5 weight % to 20 weight % of the dosage form. Binders are generally used to impart cohesive qualities to a tablet formulation. Suitable binders include microcrystalline cellulose, gelatin, sugars, polyethylene glycol, natural and synthetic gums, polyvinylpyrrolidone, pregelatinized starch, hydroxypropyl cellulose and hydroxypropyl methylcellulose. Tablets may also contain diluents, such as lactose (monohydrate, spray-dried monohydrate, anhydrous and the like), mannitol, xylitol, dextrose, sucrose, sorbitol, microcrystalline cellulose, starch and dibasic calcium phosphate dihydrate.

(53) Tablets may also optionally comprise surface active agents, such as sodium lauryl sulfate and polysorbate 80, and glidants such as silicon dioxide and talc. When present, surface active agents may comprise from 0.2 weight % to 5 weight % of the tablet, and glidants may comprise from 0.2 weight % to 1 weight % of the tablet.

(54) Tablets also generally contain lubricants such as magnesium stearate, calcium stearate, zinc stearate, sodium stearyl fumarate, and mixtures of magnesium stearate with sodium lauryl sulfate. Lubricants generally comprise from 0.25 weight % to 10 weight %, for example, from 0.5 weight % to 3 weight % of the tablet.

(55) Other possible ingredients include anti-oxidants, colorants, flavoring agents, preservatives and taste-masking agents.

(56) Exemplary tablets contain up to about 80% drug, from about 10 weight % to about 90 weight % binder, from about 0 weight % to about 85 weight % diluent, from about 2 weight % to about 10 weight % disintegrant, and from about 0.25 weight % to about 10 weight % lubricant.

(57) Tablet blends may be compressed directly or by roller to form tablets. Tablet blends or portions of blends may alternatively be wet-, dry-, or melt-granulated, melt-congealed, or extruded before tabletting. The final formulation may comprise one or more layers and may be coated or uncoated; it may even be encapsulated.

(58) The formulation of tablets is discussed in Pharmaceutical Dosage Forms: Tablets, Vol. 1, by H. Lieberman and L. Lachman (Marcel Dekker, New York, 1980).

(59) Consumable oral films for human or veterinary use are typically pliable water-soluble or water-swellable thin film dosage forms which may be rapidly dissolving or mucoadhesive and typically comprise a compound of Formula I, a film-forming polymer, a binder, a solvent, a humectant, a plasticizer, a stabilizer or emulsifier, a viscosity-modifying agent and a solvent. Some components of the formulation may perform more than one function.

(60) The compound of Formula I (or pharmaceutically acceptable salts thereof or N-oxides thereof) may be water-soluble or insoluble. A water-soluble compound typically comprises from 1 weight % to 80 weight %, more typically from 20 weight % to 50 weight %, of the solutes. Less soluble compounds may comprise a smaller proportion of the composition, typically up to 30 weight % of the solutes. Alternatively, the compound of Formula I may be in the form of multiparticulate beads.

(61) The film-forming polymer may be selected from natural polysaccharides, proteins, or synthetic hydrocolloids and is typically present in the range 0.01 to 99 weight %, more typically in the range 30 to 80 weight %.

(62) Other possible ingredients include anti-oxidants, colorants, flavorings and flavor enhancers, preservatives, salivary stimulating agents, cooling agents, co-solvents (including oils), emollients, bulking agents, anti-foaming agents, surfactants and taste-masking agents.

(63) Films in accordance with the invention are typically prepared by evaporative drying of thin aqueous films coated onto a peelable backing support or paper. This may be done in a drying oven or tunnel, typically a combined coater dryer, or by freeze-drying or vacuuming.

(64) Solid formulations for oral administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.

(65) Suitable modified release formulations for the purposes of the invention are described in U.S. Pat. No. 6,106,864. Details of other suitable release technologies such as high energy dispersions and osmotic and coated particles are to be found in Verma et al., Pharmaceutical Technology On-line, 25(2), 1-14 (2001). The use of chewing gum to achieve controlled release is described in WO 00/35298.

(66) The compounds of the invention (including pharmaceutically acceptable salts thereof) may also be administered directly into the bloodstream, into muscle, or into an internal organ. Suitable means for parenteral administration include intravenous, intraarterial, intraperitoneal, intrathecal, intraventricular, intraurethral, intrasternal, intracranial, intramuscular, intrasynovial and subcutaneous. Suitable devices for parenteral administration include needle (including microneedle) injectors, needle-free injectors and infusion techniques.

(67) Parenteral formulations are typically aqueous solutions which may contain excipients such as salts, carbohydrates and buffering agents (for example to a pH of from 3 to 9), but, for some applications, they may be more suitably formulated as a sterile non-aqueous solution or as a dried form to be used in conjunction with a suitable vehicle such as sterile, pyrogen-free water.

(68) The preparation of parenteral formulations under sterile conditions, for example, by lyophilization, may readily be accomplished using standard pharmaceutical techniques well known to those skilled in the art.

(69) The solubility of compounds of Formula I (including pharmaceutically acceptable salts thereof) used in the preparation of parenteral solutions may be increased by the use of appropriate formulation techniques, such as the incorporation of solubility-enhancing agents.

(70) Formulations for parenteral administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release. Thus compounds of the invention may be formulated as a suspension or as a solid, semi-solid, or thixotropic liquid for administration as an implanted depot providing modified release of the active compound. Examples of such formulations include drug-coated stents and semi-solids and suspensions comprising drug-loaded poly(DL-lactic-coglycolic acid) (PLGA) microspheres.

(71) The compounds of the invention (including pharmaceutically acceptable salts thereof) may also be administered topically, (intra)dermally, or transdermally to the skin or mucosa. Typical formulations for this purpose include gels, hydrogels, lotions, solutions, creams, ointments, dusting powders, dressings, foams, films, skin patches, wafers, implants, sponges, fibers, bandages and microemulsions. Liposomes may also be used. Typical carriers include alcohol, water, mineral oil, liquid petrolatum, white petrolatum, glycerin, polyethylene glycol and propylene glycol. Penetration enhancers may be incorporated. See e.g., Finnin and Morgan, J. Pharm. Sci. 1999, 88, 955-958.

(72) Other means of topical administration include delivery by electroporation, iontophoresis, phonophoresis, sonophoresis and microneedle or needle-free (e.g., Powderject, Bioject, etc.) injection.

(73) Formulations for topical administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.

(74) The compounds of the invention (including pharmaceutically acceptable salts thereof) can also be administered intranasally or by inhalation, typically in the form of a dry powder (either alone; as a mixture, for example, in a dry blend with lactose; or as a mixed component particle, for example, mixed with phospholipids, such as phosphatidylcholine) from a dry powder inhaler, as an aerosol spray from a pressurized container, pump, spray, atomizer (for example an atomizer using electrohydrodynamics to produce a fine mist), or nebulizer, with or without the use of a suitable propellant, such as 1,1,1,2-tetrafluoroethane or 1,1,1,2,3,3,3-heptafluoropropane, or as nasal drops. For intranasal use, the powder may comprise a bioadhesive agent, for example, chitosan or cyclodextrin.

(75) The pressurized container, pump, spray, atomizer, or nebulizer contains a solution or suspension of the compound(s) of the invention comprising, for example, ethanol, aqueous ethanol, or a suitable alternative agent for dispersing, solubilizing, or extending release of the active, a propellant(s) as solvent and an optional surfactant, such as sorbitan trioleate, oleic acid, or an oligolactic acid.

(76) Prior to use in a dry powder or suspension formulation, the drug product is micronized to a size suitable for delivery by inhalation (typically less than 5 microns). This may be achieved by any appropriate comminuting method, such as spiral jet milling, fluid bed jet milling, supercritical fluid processing to form nanoparticles, high pressure homogenization, or spray drying.

(77) Capsules (made, for example, from gelatin or hydroxypropyl methyl cellulose), blisters and cartridges for use in an inhaler or insufflator may be formulated to contain a powder mix of the compound of the invention, a suitable powder base such as lactose or starch and a performance modifier such as L-leucine, mannitol, or magnesium stearate. The lactose may be anhydrous or in the form of the monohydrate. Other suitable excipients include dextran, glucose, maltose, sorbitol, xylitol, fructose, sucrose and trehalose.

(78) A suitable solution formulation for use in an atomizer using electrohydrodynamics to produce a fine mist may contain from 1 g to 20 mg of the compound of the invention per actuation and the actuation volume may vary from 1 L to 100 L. A typical formulation may comprise a compound of Formula I or a pharmaceutically acceptable salt thereof, propylene glycol, sterile water, ethanol and sodium chloride. Alternative solvents which may be used instead of propylene glycol include glycerol and polyethylene glycol.

(79) Suitable flavors, such as menthol and levomenthol, or sweeteners, such as saccharin or saccharin sodium, may be added to those formulations of the invention intended for inhaled/intranasal administration.

(80) Formulations for inhaled/intranasal administration may be formulated to be immediate and/or modified release using, for example, PGLA. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.

(81) In the case of dry powder inhalers and aerosols, the dosage unit is determined by means of a valve which delivers a metered amount. Units in accordance with the invention are typically arranged to administer a metered dose or puff containing from 0.01 to 100 mg of the compound of Formula I. The overall daily dose will typically be in the range 1 g to 200 mg, which may be administered in a single dose or, more usually, as divided doses throughout the day.

(82) The compounds of the invention (including pharmaceutically acceptable salts thereof) may be administered rectally or vaginally, for example, in the form of a suppository, pessary, or enema. Cocoa butter is a traditional suppository base, but various alternatives may be used as appropriate.

(83) Formulations for rectal/vaginal administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted and programmed release.

(84) The compounds of the invention (including pharmaceutically acceptable salts thereof) may also be administered directly to the eye or ear, typically in the form of drops of a micronized suspension or solution in isotonic, pH-adjusted, sterile saline. Other formulations suitable for ocular and aural administration include ointments, gels, biodegradable (e.g., absorbable gel sponges, collagen) and non-biodegradable (e.g., silicone) implants, wafers, lenses and particulate or vesicular systems, such as niosomes or liposomes. A polymer such as crossed-linked polyacrylic acid, polyvinylalcohol, hyaluronic acid, a cellulosic polymer, for example, hydroxypropyl methyl cellulose, hydroxyethyl cellulose, or methyl cellulose, or a heteropolysaccharide polymer, for example, gelan gum, may be incorporated together with a preservative, such as benzalkonium chloride. Such formulations may also be delivered by iontophoresis.

(85) Formulations for ocular/aural administration may be formulated to be immediate and/or modified release. Modified release formulations include delayed-, sustained-, pulsed-, controlled-, targeted, or programmed release.

(86) The compounds of the invention (including pharmaceutically acceptable salts thereof) may be combined with soluble macromolecular entities, such as cyclodextrin and suitable derivatives thereof or polyethylene glycol-containing polymers, in order to improve their solubility, dissolution rate, taste-masking, bioavailability and/or stability for use in any of the aforementioned modes of administration.

(87) Drug-cyclodextrin complexes, for example, are found to be generally useful for most dosage forms and administration routes. Both inclusion and non-inclusion complexes may be used. As an alternative to direct complexation with the drug, the cyclodextrin may be used as an auxiliary additive, i.e., as a carrier, diluent, or solubilizer. Most commonly used for these purposes are alpha-, beta- and gamma-cyclodextrins, examples of which may be found in International Patent Applications Nos. WO 91/11172, WO 94/02518 and WO 98/55148.

(88) Since the present invention has an aspect that relates to the treatment of the disease/conditions described herein with a combination of active ingredients which may be administered separately, the invention also relates to combining separate pharmaceutical compositions in kit form. The kit comprises two separate pharmaceutical compositions: a compound of Formula I, a prodrug thereof, or a salt of such compound or prodrug; and a second compound as described above. The kit comprises means for containing the separate compositions such as a container, a divided bottle or a divided foil packet. Typically the kit comprises directions for the administration of the separate components. The kit form is particularly advantageous when the separate components are for example administered in different dosage forms (e.g., oral and parenteral), are administered at different dosage intervals, or when titration of the individual components of the combination is desired by the prescribing physician.

(89) An example of such a kit is a so-called blister pack. Blister packs are well known in the packaging industry and are being widely used for the packaging of pharmaceutical unit dosage forms (tablets, capsules, and the like). Blister packs generally consist of a sheet of relatively stiff material covered with a foil of a transparent plastic material. During the packaging process recesses are formed in the plastic foil. The recesses have the size and shape of the tablets or capsules to be packed. Next, the tablets or capsules are placed in the recesses and the sheet of relatively stiff material is sealed against the plastic foil at the face of the foil which is opposite from the direction in which the recesses were formed. As a result, the tablets or capsules are sealed in the recesses between the plastic foil and the sheet. In some embodiments, the strength of the sheet is such that the tablets or capsules can be removed from the blister pack by manually applying pressure on the recesses whereby an opening is formed in the sheet at the place of the recess. The tablet or capsule can then be removed via said opening.

(90) It may be desirable to provide a memory aid on the kit, e.g., in the form of numbers next to the tablets or capsules whereby the numbers correspond with the days of the regimen on which the tablets or capsules so specified should be ingested. Another example of such a memory aid is a calendar printed on the card, e.g., as follows First Week, Monday, Tuesday, etc. . . . Second Week, Monday, Tuesday, . . . etc. Other variations of memory aids will be readily apparent. A daily dose can be a single tablet or capsule or several pills or capsules to be taken on a given day. Also, a daily dose of Formula I compound can consist of one tablet or capsule while a daily dose of the second compound can consist of several tablets or capsules and vice versa. The memory aid should reflect this.

(91) In another specific embodiment of the invention, a dispenser designed to dispense the daily doses one at a time in the order of their intended use is provided. For example, the dispenser is equipped with a memory aid, so as to further facilitate compliance with the regimen. An example of such a memory aid is a mechanical counter which indicates the number of daily doses that has been dispensed. Another example of such a memory aid is a battery-powered micro-chip memory coupled with a liquid crystal readout, or audible reminder signal which, for example, reads out the date that the last daily dose has been taken and/or reminds one when the next dose is to be taken.

(92) The invention will be described in greater detail by way of specific examples. The following examples are offered for illustrative purposes, and are not intended to limit the invention in any manner. Those of skill in the art will readily recognize a variety of non-critical parameters that can be changed or modified to yield essentially the same results. Additional compounds within the scope of this invention may be prepared using the methods illustrated in these Examples, either alone or in combination with techniques generally known in the art. In the following Examples and Preparations, DMSO means dimethyl sulfoxide, N where referring to concentration means Normal, M means molar, mL means milliliter, mmol means millimoles, pmol means micromoles, eq. means equivalent, C. means degrees Celsius, MHz means megahertz, HPLC means high-performance liquid chromatography.

EXAMPLES

(93) The following illustrate the synthesis of various compounds of the present invention. Additional compounds within the scope of this invention may be prepared using the methods illustrated in these Examples, either alone or in combination with techniques generally known in the art.

(94) Experiments were generally carried out under inert atmosphere (nitrogen or argon), particularly in cases where oxygen- or moisture-sensitive reagents or intermediates were employed. Commercial solvents and reagents were generally used without further purification. Anhydrous solvents were employed where appropriate, generally AcroSeal products from Acros Organics or DriSolv products from EMD Chemicals. In other cases, commercial solvents were passed through columns packed with 4 molecular sieves, until the following QC standards for water were attained: a) <100 ppm for dichloromethane, toluene, N,N-dimethylformamide and tetrahydrofuran; b) <180 ppm for methanol, ethanol, 1,4-dioxane and diisopropylamine. For very sensitive reactions, solvents were further treated with metallic sodium, calcium hydride or molecular sieves, and distilled just prior to use. Products were generally dried under vacuum before being carried on to further reactions or submitted for biological testing. Mass spectrometry data is reported from either liquid chromatography-mass spectrometry (LCMS), atmospheric pressure chemical ionization (APCI) or gas chromatography-mass spectrometry (GCMS) instrumentation. Chemical shifts for nuclear magnetic resonance (NMR) data are expressed in parts per million (ppm, 6) referenced to residual peaks from the deuterated solvents employed. In some examples, chiral separations were carried out to separate enantiomers or diastereomers of certain compounds of the invention (in some examples, the separated enantiomers are designated as ENT-1 and ENT-2, or the separated diastereomers are designated as DIAST-1 and DIAST-2, according to their order of elution). In some examples, the optical rotation of an enantiomer was measured using a polarimeter. According to its observed rotation data (or its specific rotation data), an enantiomer with a clockwise rotation was designated as the (+)-enantiomer and an enantiomer with a counter-clockwise rotation was designated as the ()-enantiomer. Racemic compounds are indicated by the presence of (+/) adjacent to the structure; in these cases, indicated stereochemistry represents the relative (rather than absolute) configuration of the compound's substituents.

(95) Reactions proceeding through detectable intermediates were generally followed by LCMS, and allowed to proceed to full conversion prior to addition of subsequent reagents. For syntheses referencing procedures in other Examples or Methods, reaction conditions (reaction time and temperature) may vary. In general, reactions were followed by thin-layer chromatography or mass spectrometry, and subjected to work-up when appropriate. Purifications may vary between experiments: in general, solvents and the solvent ratios used for eluents/gradients were chosen to provide appropriate R.sub.fs or retention times.

(96) For clarity purposes, the stereochemistry of the substituents on the 3-azabicyclo[3.1.0]hexyl skeleton in Examples and intermediates herein is indicated by using Chemical Abstracts nomenclature. The stereochemistry of the other compounds in the Examples and intermediates herein is indicated by using IUPAC nomenclature.

Abbreviations

(97) BOCtert-butoxycarbonyl

(98) HPLChigh-performance liquid chromatography

(99) NADPnicotinamide adenine dinucleotide phosphate

(100) PMBpara-methoxybenzyl (or 4-methoxybenzyl)

(101) p-TsOHpara-toluenesulfonic acid, 4-methylbenzenesulfonic acid

(102) psipounds per square inch

Example 1

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[I-(5-methoxypyridin-2-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (1)

(103) ##STR00040## ##STR00041## ##STR00042##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-ol (C1)

(104) (4-Methoxyphenyl)methanol (98%, 1.14 mL, 8.96 mmol) was slowly added to a 0 C. solution of sodium bis(trimethylsilyl)amide in tetrahydrofuran (1.0 M, 8.9 mL, 8.9 mmol) in a microwave vial. After the reaction mixture had stirred at 0 C. for 45 minutes, (2R)-2-(trifluoromethyl)oxirane (500 mg, 4.46 mmol) in tetrahydrofuran (2 mL) was added via syringe, and the vial was sealed and heated at 100 C. for 18 hours. The reaction mixture was then cooled to room temperature and diluted with water; the mixture was extracted twice with tert-butyl methyl ether and the combined organic layers were washed with saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered, and concentrated in vacuo. Purification via chromatography on silica gel (Gradient: 0% to 60% ethyl acetate in heptane) afforded the product as a pale yellow oil. Yield: 1.09 g, 4.36 mmol, 98%. GCMS m/z 250.1 [M.sup.+]. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.26 (d, J=8.5 Hz, 2H), 6.91 (d, J=8.5 Hz, 2H), 6.36 (d, J=6.7 Hz, 1H), 4.46 (s, 2H), 4.21-4.09 (m, 1H), 3.74 (s, 3H), 3.58 (dd, half of ABX pattern, J=10.6, 4.5 Hz, 1H), 3.48 (dd, half of ABX pattern, J=10.5, 6.3 Hz, 1H).

Step 2. Synthesis of pentafluorophenyl (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl carbonate (C2)

(105) Bis(pentafluorophenyl) carbonate (1.33 g, 3.37 mmol) was added to a 0 C. solution of C1 (929 mg, 3.71 mmol) in acetonitrile (30 mL). Triethylamine (1.71 g, 16.9 mmol) was added in a drop-wise manner, and the reaction was warmed to 25 C. and stirred for 2 hours. The resulting solution of C2 was used directly in Step 11. For subsequent syntheses described herein that utilize C2, this material was generated at the appropriate scale, and the reaction solution of C2 was used directly in the coupling reaction.

Step 3. Synthesis of tert-butyl (1,5,6)-6-[methoxy(methyl)carbamoyl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (C3)

(106) 1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (10.1 g, 52.7 mmol) and 1H-benzotriazol-1-ol (7.13 g, 52.8 mmol) were added to a 0 C. solution of (1,5,6)-3-(tert-butoxycarbonyl)-3-azabicyclo[3.1.0]hexane-6-carboxylic acid (8.00 g, 35 mmol) in dichloromethane (80 mL), and the reaction mixture was stirred at 0 C. for 30 minutes. A solution of N-methoxymethanamine hydrochloride (6.87 g, 70.4 mmol) and N,N-diisopropylethylamine (13.6 g, 105 mmol) in dichloromethane (50 mL) was then added drop-wise over a period of 10 minutes, and the reaction mixture was stirred at room temperature (25 C.) for 2 hours. After addition of water (100 mL), the mixture was extracted with dichloromethane (3100 mL), and the combined organic layers were washed with water (50 mL) and with saturated aqueous sodium chloride solution (50 mL), dried over sodium sulfate, filtered, and concentrated in vacuo to provide the product as a light yellow oil. Yield: 9.46 g, 35.0 mmol, 100%. .sup.1H NMR (400 MHz, CDCl.sub.3) 3.72 (s, 3H), 3.64 (d, half of AB quartet, J=11.2 Hz, 1H), 3.55 (d, half of AB quartet, J=11.0 Hz, 1H), 3.49-3.39 (m, 2H), 3.18 (s, 3H), 2.11-1.99 (m, 2H), 1.99-1.91 (br s, 1H), 1.43 (s, 9H).

Step 4. Synthesis of tert-butyl (1,5,6)-6-acetyl-3-azabicyclo[3.1.0]hexane-3-carboxylate (C4)

(107) Methylmagnesium bromide (3.0 M solution in tetrahydrofuran; 23.3 mL, 69.9 mmol) was added in a drop-wise manner to a 0 C. solution of C3 (9.46 g, 35.0 mmol) in tetrahydrofuran (100 mL). The reaction mixture was stirred at room temperature (25 C.) for 1 hour, whereupon it was quenched with saturated aqueous ammonium chloride solution (200 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were washed sequentially with water (100 mL) and with saturated aqueous sodium chloride solution (100 mL), dried over sodium sulfate, filtered, and concentrated in vacuo to provide the product as a red solid. Yield: 7.82 g, 34.7 mmol, 99%. .sup.1H NMR (400 MHz, CDCl.sub.3) 3.62 (d, half of AB quartet, J=11.3 Hz, 1H), 3.53 (d, half of AB quartet, J=11.3 Hz, 1H), 3.41-3.32 (m, 2H), 2.21 (s, 3H), 2.05-2.01 (m, 2H), 1.77 (dd, J=3.0, 2.9 Hz, 1H), 1.39 (s, 9H).

Step 5. Synthesis of tert-butyl (1,5,6)-6-[(2E)-3-(dimethylamino)prop-2-enoyl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (C5)

(108) To a solution of C4 (7.82 g, 34.7 mmol) in N,N-dimethylformamide (50 mL) was added N,N-dimethylformamide dimethyl acetal (12.4 g, 104 mmol), and the reaction mixture was stirred at 110 C. for 16 hours. It was then cooled, treated with water (100 mL), and extracted with ethyl acetate (3100 mL). The combined organic layers were washed sequentially with water (3100 mL) and with saturated aqueous sodium chloride solution (90 mL), dried over sodium sulfate, filtered, and concentrated under reduced pressure to afford the product as a red solid. Yield: 9.20 g, 32.8 mmol, 94%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.51 (d, J=12.7 Hz, 1H), 5.13 (d, J=12.7 Hz, 1H), 3.63 (d, half of AB quartet, J=11.2 Hz, 1H), 3.54 (d, half of AB quartet, J=11.0 Hz, 1H), 3.44-3.36 (m, 2H), 3.15-2.93 (br s, 3H), 2.93-2.70 (br s, 3H), 2.10-1.97 (m, 2H), 1.60 (dd, J=2.9, 2.9 Hz, 1H), 1.42 (s, 9H).

Step 6. Synthesis of tert-butyl (1,5,6)-6-(H-pyrazol-3-yl)-3-azabicyclo[3.1.0]hexane-3-carboxylate (C6)

(109) Hydrazine hydrate (1.97 g, 39.4 mmol) was added to a solution of C5 (9.20 g, 32.8 mmol) in ethanol (100 mL), and the reaction mixture was stirred at 80 C. for 16 hours. After concentration in vacuo, the residue was purified by chromatography on silica gel (Eluents: 9%, then 17%, then 50% ethyl acetate in diethyl ether) to afford the product as a white solid. Yield: 7.00 g, 28.1 mmol, 86%. LCMS m/z 193.8 [(M-2-methylprop-1-ene)+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.47 (d, J=2.0 Hz, 1H), 6.01 (br d, J=1.8 Hz, 1H), 3.78 (d, J=10.9 Hz, 1H), 3.69 (d, J=11.0 Hz, 1H), 3.51-3.41 (m, 2H), 1.90-1.83 (m, 2H), 1.80 (dd, J=3.4, 3.4 Hz, 1H), 1.46 (s, 9H).

Step 7. Synthesis of tert-butyl (1,5,6)-6-[1-(5-bromopyridin-2-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (C7)

(110) A mixture of C6 (500 mg, 2.01 mmol), 5-bromo-2-fluoropyridine (529 mg, 3.01 mmol) and cesium carbonate (1.96 g, 6.02 mmol) in N,N-dimethylformamide (20 mL) was stirred in a microwave reactor at 160 C. for 1 hour. The reaction mixture was then combined with two similar reactions carried out on C6 (500 mg, 2.01 mmol, and 350 mg, 1.40 mmol), diluted with water (100 mL), and extracted with ethyl acetate (350 mL); the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Purification via silica gel chromatography (Gradient: 0% to 20% ethyl acetate in petroleum ether) afforded the product as a white solid. Yield: 1.25 g, 3.08 mmol, 57%. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.41 (dd, J=2.4, 0.6 Hz, 1H), 8.37 (d, J=2.6 Hz, 1H), 7.87 (dd, half of ABX pattern, J=8.7, 2.3 Hz, 1H), 7.80 (dd, half of ABX pattern, J=8.7, 0.7 Hz, 1H), 6.16 (d, J=2.6 Hz, 1H), 3.80 (d, J=11.0 Hz, 1H), 3.72 (d, J=11.0 Hz, 1H), 3.52-3.42 (m, 2H), 1.99-1.91 (m, 2H), 1.85 (dd, J=3.5, 3.4 Hz, 1H), 1.47 (s, 9H).

Step 8. Synthesis of tert-butyl (1,5,6)-6-{1-[5-(4, 4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl]-1H-pyrazol-3-yl}-3-azabicyclo[3.1.0]hexane-3-carboxylate (C8)

(111) To a suspension of C7 (1.00 g, 2.47 mmol) in toluene (20 mL) were added 4,4,4,4,5,5,5,5-octamethyl-2,2-bi-1,3,2-dioxaborolane (940 mg, 3.70 mmol), [1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (181 mg, 0.247 mmol), and potassium acetate (726 mg, 7.40 mmol), and the mixture was degassed with nitrogen for 5 minutes. The reaction mixture was stirred for 18 hours at 120 C., whereupon it was concentrated in vacuo and purified by chromatography on silica gel (Gradient: 0% to 20% ethyl acetate in petroleum ether) to afford the product as a white solid. Yield: 1.02 g, 2.25 mmol, 91%. LCMS m/z 453.3 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.73-8.69 (m, 1H), 8.48 (d, J=2.5 Hz, 1H), 8.14 (dd, J=8.2, 1.8 Hz, 1H), 7.86 (br d, J=8.2 Hz, 1H), 6.16 (d, J=2.5 Hz, 1H), 3.81 (d, J=11 Hz, 1H), 3.73 (d, J=11 Hz, 1H), 3.53-3.42 (m, 2H), 2.01-1.93 (m, 2H), 1.87 (dd, J=3.3, 3.3 Hz, 1H), 1.47 (s, 9H), 1.37 (s, 12H).

Step 9. Synthesis of tert-butyl (1,5,6)-6-[1-(5-hydroxypyridin-2-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (C9)

(112) To a 0 C. mixture of C8 (1.02 g, 2.25 mmol) in tetrahydrofuran and water (1:1 mixture, 80 mL) was added aqueous sodium hydroxide solution (6 M, 1 mL, 6 mmol), followed by hydrogen peroxide (30% solution in water, 0.77 g, 6.8 mmol). The reaction mixture was allowed to warm to 25 C., and was stirred for 12 hours, whereupon it was quenched with aqueous sodium thiosulfate solution, acidified to pH 6 with aqueous hydrochloric acid, and extracted with dichloromethane (330 mL). The combined organic layers were concentrated under reduced pressure and purified via chromatography on silica gel (Gradient: 0% to 10% methanol in dichloromethane) to provide the product as a white solid. Yield: 742 mg, 2.17 mmol, 96%. LCMS m/z 343.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD) 8.26 (d, J=2.6 Hz, 1H), 7.93 (dd, J=2.9, 0.5 Hz, 1H), 7.67 (dd, J=8.8, 0.5 Hz, 1H), 7.32 (dd, J=8.8, 2.9 Hz, 1H), 6.21 (d, J=2.5 Hz, 1H), 3.69 (d, J=10.9 Hz, 2H), 3.52-3.42 (m, 2H), 2.02-1.94 (m, 2H), 1.75 (dd, J=3.5, 3.4 Hz, 1H), 1.47 (s, 9H).

Step 10. Synthesis of 6-{3-[(1,5,6)-3-azabicyclo[3.1.0]hex-6-yl]-1H-pyrazol-1-yl}pyridin-3-ol, tris(trifluoroacetic acid) salt (C10)

(113) A solution of C9 (742 mg, 2.17 mmol) in dichloromethane (5 mL) was cooled in an ice bath, and then treated with trifluoroacetic acid (3 mL). The reaction mixture was stirred for 30 minutes at 25 C., whereupon it was concentrated in vacuo, affording the product (1.27 g) as a yellow gum. LCMS m/z 243.0 [M+H].sup.+.

Step 11. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (1,5,6)-6-[1-(5-hydroxypyridin-2-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (C11)

(114) Triethylamine (1.10 g, 10.9 mmol) was slowly added to a 0 C. solution of C10 (from the previous step, 1.27 g, 2.17 mmol) in acetonitrile (20 mL), whereupon the mixture was stirred for 1 hour. Compound C2 [from step 2, as the crude reaction mixture in acetonitrile (30 mL); 1.6 g, 3.4 mmol] was added to the 0 C. reaction mixture, which was then stirred at 28 C. for 18 hours. It was then cooled in an ice-water bath and slowly treated with a second batch of C2 (0.74 g, 1.6 mmol). After stirring for 18 hours at 25 C., the reaction mixture was concentrated in vacuo; the residue was purified via chromatography on silica gel (Gradient: 0% to 30% ethyl acetate in petroleum ether) to provide the product as a white solid. By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. Yield: 430 mg, 0.83 mmol, 38% over two steps. LCMS m/z 519.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD) 8.29-8.25 (m, 1H), 7.93 (d, J=2.9 Hz, 1H), [7.69 (d, J=8.8 Hz) and 7.68 (d, J=8.9 Hz), total 1H], 7.32 (dd, J=8.8, 2.9 Hz, 1H), 7.29-7.23 (m, 2H), 6.95-6.88 (m, 2H), [6.24 (d, J=2.5 Hz) and 6.20 (d, J=2.5 Hz), total 1H], 5.53-5.40 (m, 1H), [4.56 (d, half of AB quartet, J=11.4 Hz) and 4.54 (d, half of AB quartet, J=11.5 Hz), total 1H], 4.46 (d, half of AB quartet, J=11.5 Hz, 1H), 3.84-3.68 (m, 4H), [3.79 (s) and 3.73 (s), total 3H], 3.60-3.53 (m, 2H), 2.07-1.99 (m, 2H), [1.77 (dd, J=3.5, 3.4 Hz) and 1.74 (dd, J=3.5, 3.3 Hz), total 1H].

Step 12. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (1,5,6)-6-[1-(5-methoxypyridin-2-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (C12)

(115) To a 0 C. solution of C11 (90.0 mg, 0.174 mmol) in N,N-dimethylformamide (1 mL) were added potassium carbonate (36 mg, 0.26 mmol) and iodomethane (25.9 mg, 0.182 mmol). The reaction mixture was stirred at 28 C. for 2 hours, whereupon it was concentrated in vacuo and purified by silica gel chromatography (Gradient: 0% to 30% ethyl acetate in petroleum ether) to afford the product as a colorless gum. By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. Yield: 86 mg, 0.16 mmol, 92%. LCMS m/z 533.3 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.33 (d, J=2.4 Hz, 1H), 8.05 (d, J=2.8 Hz, 1H), 7.82 (d, J=8.9 Hz, 1H), 7.32 (dd, J=9.0, 2.9 Hz, 1H), 7.30-7.23 (m, 2H), 6.94-6.86 (m, 2H), [6.14 (d, J=2.3 Hz) and 6.13 (d, J=2.4 Hz), total 1H], 5.54-5.43 (m, 1H), [4.57 (d, half of AB quartet, J=11.7 Hz) and 4.56 (d, half of AB quartet, J=11.7 Hz), total 1H], 4.48 (d, half of AB quartet, J=11.7 Hz, 1H), 3.92-3.65 (m, 4H), 3.87 (s, 3H), [3.81 (s) and 3.78 (s), total 3H], 3.64-3.53 (m, 2H), 2.06-1.97 (m, 2H), 1.89-1.82 (m, 1H).

Step 13. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(5-methoxypyridin-2-yl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (1)

(116) Trifluoroacetic acid (1 mL) was slowly added to a 0 C. solution of C12 (114 mg, 0.214 mmol) in dichloromethane (2 mL). The reaction mixture was stirred at 26 C. for 30 minutes, whereupon it was cooled in an ice bath and slowly treated with saturated aqueous sodium bicarbonate solution (20 mL). The mixture was extracted with dichloromethane (320 mL), and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Preparative thin layer chromatography on silica gel (Eluent: 1:1 petroleum ether:ethyl acetate) provided the product as a white solid. By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. Yield: 65 mg, 0.16 mmol, 75%. LCMS m/z 413.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD) 8.32 (d, J=2.5 Hz, 1H), 8.07 (d, J=2.9 Hz, 1H), 7.80-7.76 (m, 1H), 7.51 (dd, J=9.0, 3.0 Hz, 1H), [6.26 (d, J=2.6 Hz) and 6.25 (d, J=2.8 Hz), total 1H], 5.34-5.24 (m, 1H), 3.94-3.74 (m, 4H), 3.90 (s, 3H), 3.70-3.57 (m, 2H), 2.11-2.02 (m, 2H), [1.86 (dd, J=3.6, 3.5 Hz) and 1.79 (dd, J=3.5, 3.4 Hz), total 1H].

Example 2

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]piperidine-1-carboxylate (2)

(117) ##STR00043## ##STR00044##

Step 1. Synthesis of tert-butyl 4-(1H-pyrazol-3-yl)piperidine-1-carboxylate (C13)

(118) To a 0 C. mixture of 4-(1H-pyrazol-3-yl)piperidine, dihydrochloride salt (11.3 g, 50.4 mmol) and triethylamine (20.4 g, 202 mmol) in dichloromethane (250 mL) was slowly added di-tert-butyl dicarbonate (11.0 g, 50.4 mmol), and the reaction mixture was allowed to stir at room temperature overnight. It was then concentrated under reduced pressure and purified using silica gel chromatography (Gradient: 17% to 80% ethyl acetate in petroleum ether), providing the product as a light yellow gum. Yield: 9.50 g, 37.8 mmol, 75%. LCMS m/z 195.8 [(M-2-methylprop-1-ene)+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.50 (br s, 1H), 6.12 (br s, 1H), 4.30-4.03 (br s, 2H), 2.96-2.73 (m, 3H), 2.04-1.86 (m, 2H), 1.73-1.55 (m, 2H), 1.48 (s, 9H).

Step 2. Synthesis of tert-butyl 4-[I-(4-fluorophenyl)-1H-pyrazol-3-yl]piperidine-1-carboxylate (C14)

(119) To a mixture of C13 (700 mg, 2.78 mmol), (4-fluorophenyl)boronic acid (429 mg, 3.07 mmol), and 4 molecular sieves (1.0 g) in dry dichloromethane (40 mL) were added pyridine (441 mg, 5.58 mmol) and copper(II) acetate (759 mg, 4.18 mmol). The reaction mixture was stirred for 48 hours at room temperature, while open to the air, and was then filtered. The filtrate was poured into water and extracted with dichloromethane (350 mL); the combined organic layers were washed sequentially with water (100 mL) and with saturated aqueous sodium chloride solution (100 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. Purification via chromatography on silica gel (Eluent: 25% ethyl acetate in petroleum ether) afforded the product as a white solid. Yield: 700 mg, 2.0 mmol, 72%. .sup.1H NMR (400 MHz, CD.sub.3OD) 8.05 (br d, J=2.5 Hz, 1H), 7.74-7.68 (m, 2H), 7.24-7.17 (m, 2H), 6.37 (br d, J=2.5 Hz, 1H), 4.19-4.10 (m, 2H), 3.02-2.85 (m, 3H), 2.02-1.92 (m, 2H), 1.71-1.58 (m, 2H), 1.48 (s, 9H).

Step 3. Synthesis of 4-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]piperidine, hydrochloride salt (C15)

(120) A solution of hydrogen chloride in ethyl acetate (4 M, 10 mL, 40 mmol) was added to a 0 C. solution of C14 (700 mg, 2.0 mmol) in ethyl acetate (10 mL). After the reaction mixture had been stirred for 1.5 hours at room temperature (18 C.), it was concentrated in vacuo to provide the product as a white solid. This material was used without further purification. Yield: 560 mg, assumed quantitative. .sup.1H NMR (400 MHz, CD.sub.3OD) 8.13-8.10 (m, 1H), 7.77-7.70 (m, 2H), 7.26-7.19 (m, 2H), 6.44-6.42 (m, 1H), 3.49 (ddd, J=13, 4, 4 Hz, 2H), 3.22-3.07 (m, 3H), 2.31-2.22 (m, 2H), 2.06-1.93 (m, 2H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]piperidine-1-carboxylate (C16)

(121) To a 0 C. solution of bis(trichloromethyl) carbonate (27.2 mg, 91.6 mol) in dichloromethane (5 mL) was added C1 (69.4 mg, 0.277 mmol), followed by N,N-diisopropylethylamine (36 mg, 0.28 mmol) and 4-(dimethylamino)pyridine (2.0 mg, 16 mol). After the reaction mixture had stirred at room temperature (15 C.) for 7 hours, it was cooled to 0 C. and treated with a solution of C15 (100 mg, 0.408 mmol) and N,N-diisopropylethylamine (72 mg, 0.56 mmol) in dichloromethane (5 mL). The reaction mixture was then stirred at 15 C. for 16 hours, whereupon it was diluted with dichloromethane (10 mL) and washed sequentially with water (320 mL) and with saturated aqueous sodium chloride solution (220 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. Silica gel chromatography (Eluent: 20% ethyl acetate in petroleum ether) provided the product as a colorless oil, which was not pure via .sup.1H NMR analysis. Yield: 90 mg, 560%. .sup.1H NMR (400 MHz, CD.sub.3OD), characteristic peaks: 8.04 (d, J=2.5 Hz, 1H), 7.74-7.68 (m, 2H), 7.20 (br dd, J=8.8, 8.8 Hz, 2H), 3.79 (s, 3H), 3.15-2.92 (m, 3H), 2.06-1.95 (m, 2H), 1.75-1.61 (m, 2H).

Step 5. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]piperidine-1-carboxylate (2)

(122) To a solution of C16 (50 mg, 96 mol) in ethanol (50 mL) was added palladium on carbon (30 mg), and the reaction mixture was stirred at 20 C. under hydrogen (40 psi) for 6 hours. It was then filtered through a pad of diatomaceous earth, and the filtrate was concentrated in vacuo; purification via preparative thin layer chromatography on silica gel (Eluent: 25% ethyl acetate in petroleum ether) afforded the product as a white solid. Yield: 15 mg, 37 mol, 38%. LCMS m/z 402.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD) 8.06 (d, J=2.5 Hz, 1H), 7.74-7.68 (m, 2H), 7.21 (br dd, J=9.0, 8.4 Hz, 2H), 6.38 (d, J=2.5 Hz, 1H), 5.32 (dqd, J=7, 7, 4 Hz, 1H), 4.29-4.16 (br m, 2H), 3.89 (br dd, half of ABX pattern, J=12.5, 4 Hz, 1H), 3.79 (br dd, half of ABX pattern, J=12.4, 6.9 Hz, 1H), 3.19-3.0 (m, 2H), 2.98 (tt, J=11.5, 4 Hz, 1H), 2.08-1.96 (m, 2H), 1.85-1.61 (br m, 2H).

Example 3

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (3)

(123) ##STR00045##

Step 1. Synthesis of 1-{[({(2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl}oxy) carbonyl]oxy}pyrrolidine-2,5-dione (C17)

(124) To a solution of C1 (701 mg, 2.80 mmol) in dichloromethane (20 mL) were added triethylamine (850 mg, 8.40 mmol) and 1,1-[carbonylbis(oxy)]dipyrrolidine-2,5-dione (717 mg, 2.80 mmol). The reaction mixture was stirred for 18 hours at 25 C., then used directly in Step 4. For subsequent syntheses described herein that utilize C17, this material was generated at the appropriate scale, and the reaction solution of C17 was used directly in the coupling reaction.

Step 2. Synthesis of tert-butyl (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (C18)

(125) To a 15 C. solution of C6 (4.0 g, 16 mmol) in dichloromethane (300 mL) were added (4-fluorophenyl)boronic acid (2.92 g, 20.9 mmol), copper(II) acetate (4.37 g, 24.1 mmol), pyridine (3.81 g, 48.2 mmol), and 4 molecular sieves (0.5 g). The reaction mixture was stirred for 18 hours at 30 C., whereupon it was washed with aqueous ammonium hydroxide solution (100 mL). This aqueous layer was extracted with dichloromethane (2100 mL), and the combined organic layers were washed with saturated aqueous sodium chloride solution (150 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. Silica gel chromatography (Gradient: 0% to 25% ethyl acetate in petroleum ether) provided the product as a white solid. Yield: 3.3 g, 9.6 mmol, 60%. LCMS m/z 287.8 [(M-2-methylprop-1-ene)+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.73 (d, J=2.5 Hz, 1H), 7.62-7.56 (m, 2H), 7.12 (br dd, J=8.9, 8.4 Hz, 2H), 6.16 (d, J=2.4 Hz, 1H), 3.80 (d, half of AB quartet, J=11.0 Hz, 1H), 3.71 (d, half of AB quartet, J=10.9 Hz, 1H), 3.52-3.42 (m, 2H), 1.99-1.90 (m, 2H), 1.85 (dd, J=3.4, 3.4 Hz, 1H), 1.47 (s, 9H).

Step 3. Synthesis of (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane, trifluoroacetate salt (C19)

(126) A mixture of C18 (1.0 g, 2.9 mmol) in trifluoroacetic acid (10 mL) was stirred for 30 minutes at 15 C., whereupon it was concentrated in vacuo. The residue was triturated with tert-butyl methyl ether (10 mL) to provide the product as a white solid, which was used directly in the following step. LCMS m/z 243.9 [M+H].sup.+.

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (C20)

(127) To a 15 C. solution of C17 [the reaction mixture from Step 1; 52.80 mmol in dichloromethane (20 mL)] was added a solution of C19 (from the previous step, 52.9 mmol) and triethylamine (566 mg, 5.59 mmol) in dichloromethane (10 mL). The reaction mixture was stirred overnight at 18 C., whereupon it was concentrated in vacuo. Purification using silica gel chromatography (Gradient: 0% to 25% ethyl acetate in petroleum ether) provided the product as a gum. By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. Yield: 900 mg, 1.7 mmol, 61% over two steps. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.74 (d, J=2.4 Hz, 1H), 7.62-7.57 (m, 2H), 7.29-7.24 (m, 2H), 7.13 (br dd, J=8.9, 8.3 Hz, 2H), 6.93-6.88 (m, 2H), [6.19 (d, J=2.4 Hz) and 6.16 (d, J=2.4 Hz), total 1H], 5.53-5.43 (m, 1H), 4.60-4.54 (m, 1H), 4.48 (d, half of AB quartet, J=11.7 Hz, 1H), 3.90-3.84 (m, 1H), 3.84-3.73 (m, 2H), [3.82 (s) and 3.79 (s), total 3H], 3.73-3.65 (m, 1H), 3.65-3.54 (m, 2H), 2.04-2.00 (m, 2H), 1.87-1.82 (m, 1H).

Step 5. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (1,5,6)-6-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate (3)

(128) Trifluoroacetic acid (10 mL) was added to a solution of C20 (890 mg, 1.7 mmol) in dichloromethane (30 mL), and the reaction mixture was stirred for 4 hours at 15 C. It was then slowly poured into saturated aqueous sodium bicarbonate solution, and the resulting mixture was extracted with dichloromethane (350 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo; purification via chromatography on silica gel (Gradient: 0% to 50% ethyl acetate in petroleum ether) afforded the product as a white solid. Yield: 440 mg, 1.1 mmol, 65%. LCMS m/z 399.9 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.74 (d, J=2.4 Hz, 1H), 7.63-7.56 (m, 2H), 7.13 (dd, J=8.7, 8.5 Hz, 2H), 6.21-6.17 (m, 1H), 5.31-5.21 (m, 1H), 4.06-3.96 (m, 1H), 3.93-3.80 (m, 3H), 3.67-3.58 (m, 2H), 2.38-2.27 (br m, 1H), 2.08-2.01 (m, 2H), 1.90-1.84 (m, 1H).

Examples 4 and 5

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate [from C25, DIAST-1] (4) and (2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate [from C26, DIAST-2] (5)

(129) ##STR00046## ##STR00047##

Step 1. Synthesis of tert-butyl 4-{[(chloroacetyl)amino]methyl}-4-hydroxypiperidine-1-carboxylate (C21)

(130) A solution of potassium carbonate (1.32 kg, 9.55 mol) in water (11 L) was added to a solution of tert-butyl 4-(aminomethyl)-4-hydroxypiperidine-1-carboxylate (1.10 kg, 4.78 mol) in ethyl acetate (11 L). The mixture was cooled to 0 C., and then treated in a drop-wise manner with chloroacetyl chloride (595 g, 5.27 mol). After completion of the addition, the reaction mixture was warmed to 25 C. and allowed to stir for 16 hours. The aqueous layer was extracted with ethyl acetate (310 L), and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo; trituration of the residue with tert-butyl methyl ether (10 L) afforded the product (1040 g). The filtrate from the trituration was concentrated and triturated with a mixture of tert-butyl methyl ether and petroleum ether (1:1; 300 mL) to provide additional product (123 g) as a white solid. Combined yield: 1.16 kg, 3.78 mol, 79%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.02 (br t, J=5 Hz, 1H), 4.09 (s, 2H), 3.88-3.70 (br m, 2H), 3.43-3.28 (br s, 2H), 3.20 (br dd, J=11, 11 Hz, 2H), 2.71 (s, 1H), 1.62-1.46 (m, 4H), 1.45 (s, 9H).

Step 2. Synthesis of tert-butyl 3-oxo-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C22)

(131) This reaction was carried out in two similar batches. To a solution of C21 (540 g, 1.76 mol) in 2-propanol (20 L) was added potassium tert-butoxide (1.98 kg, 17.6 mol) at 25 C., and the reaction mixture was stirred at 25 C. for 16 hours. After removal of solvent in vacuo, the residue was partitioned between ethyl acetate (15 L) and water (20 L). The aqueous layer was extracted with ethyl acetate (215 L), and the combined organic layers were washed with saturated aqueous sodium chloride solution (15 L), dried over sodium sulfate, filtered, and concentrated under reduced pressure. The residue was triturated with tert-butyl methyl ether (2 L) at 25 C. for 3 hours to afford the product as a white solid. Combined yield from the two batches: 540 g, 2.00 mmol, 57%. .sup.1H NMR (400 MHz, CDCl.sub.3) 6.78-6.59 (br m, 1H), 4.16 (s, 2H), 3.96-3.74 (brs, 2H), 3.24 (d, J=2.6 Hz, 2H), 3.11 (br dd, J=12, 12 Hz, 2H), 1.89 (brd, J=13 Hz, 2H), 1.58-1.48 (m, 2H), 1.46 (s, 9H).

Step 3. Synthesis of tert-butyl 1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C23)

(132) This reaction was carried out in 12 batches, as follows. Borane-dimethyl sulfide complex (10 M in dimethyl sulfide, 75 mL, 750 mmol) was added in a drop-wise manner to a solution of C22 (50 g, 180 mmol) in tetrahydrofuran (1.5 L). The reaction mixture was heated at reflux (70 C.) for 6 hours and subsequently allowed to stir at 25 C. for 10 hours. It was then quenched with methanol (500 mL), stirred for 30 minutes at 25 C., and concentrated under reduced pressure. The resulting white solid was dissolved in methanol (1 L), treated with N,N-dimethylethane-1,2-diamine (65 g, 740 mmol), and heated at reflux (70 C.) for 16 hours. The 12 reaction mixtures were combined and concentrated in vacuo to provide a light yellow oil; this was dissolved in dichloromethane (4 L), washed with aqueous ammonium chloride solution (42 L), dried over sodium sulfate, filtered, and concentrated under reduced pressure. The residue was triturated with petroleum ether (500 mL) at 25 C. for 30 minutes to provide the product (304 g) as a white solid. The filtrate from the trituration was concentrated in vacuo, and the residue was triturated with petroleum ether (200 mL) at 25 C. for 36 hours, affording additional product (135 g) as a white solid. Combined yield: 439 g, 1.71 mol, 77%. LCMS m/z 257.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 3.85-3.59 (m, 4H), 3.14 (br dd, J=11, 11 Hz, 2H), 2.84 (dd, J=4.9, 4.6 Hz, 2H), 2.68 (s, 2H), 2.02-1.84 (br m, 2H), 1.47-1.33 (m, 2H), 1.45 (s, 9H).

Step 4. Synthesis of tert-butyl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C24)

(133) Titanium(IV) isopropoxide (998 mg, 3.51 mmol) was added to a mixture of C23 (300 mg, 1.17 mmol) and tetrahydro-2H-pyran-3-carbaldehyde (160 mg, 1.40 mmol) in ethanol (10 mL) at 27 C., and the reaction mixture was stirred at 27 C. for 15 hours. It was then cooled to 0 C., treated with sodium borohydride (88.6 mg, 2.34 mmol), and allowed to stir at 25 C. for 4 hours. Water (10 mL) was added slowly, and the resulting mixture was stirred at 25 C. for 30 minutes. After combination with a mixture derived from a smaller-scale reaction carried out on C23 (50 mg, 0.20 mmol), this was extracted with ethyl acetate (330 mL). The combined organic layers were dried, filtered, and concentrated in vacuo; purification via chromatography on silica gel (Gradient: 0% to 5% methanol in dichloromethane) provided the product as a colorless oil. Starting material C23 (200 mg) was also recovered, as a yellow gum. Yield: 106 mg, 0.299 mmol, 22% (51% based on recovered starting material). LCMS m/z 355.3 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 3.96-3.88 (m, 1H), 3.88-3.80 (m, 1H), 3.79-3.58 (m, 4H), 3.42-3.33 (m, 1H), 3.19-3.04 (m, 3H), 2.42-2.33 (m, 1H), 2.33-2.26 (m, 1H), 2.26-2.19 (m, 1H), 2.15-2.01 (m, 3H), 1.98-1.73 (m, 5H), 1.64-1.53 (m, 2H), 1.44 (s, 9H), 1.44-1.34 (m, 2H).

Step 5. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate, DIAST 1 (C25) and (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate, DIAST 2 (C26)

(134) A solution of C24 (106 mg, 0.299 mmol) in dichloromethane (2 mL) was cooled to 0 C. and treated with trifluoroacetic acid (0.5 mL). The reaction mixture was stirred at 25 C. for 50 minutes, whereupon it was concentrated in vacuo to provide 4-(tetrahydro-2H-pyran-3-ylmethyl)-1-oxa-4,9-diazaspiro[5.5]undecane, bis-trifluoroacetic acid salt as a yellow oil (100 mg). This material was taken up in acetonitrile (5 mL) and cooled to 0 C. Triethylamine (151 mg, 1.49 mmol) was added, and the reaction mixture was allowed to stir at 0 C. for a few minutes, whereupon C2 (reaction solution in acetonitrile, containing 0.49 mmol) was added drop-wise. The resulting solution was stirred at 0 C. for a few minutes, and then allowed to stir at 25 C. for 15 hours. The reaction mixture was cooled to 0 C. and treated in a drop-wise manner with additional C2 (reaction solution in acetonitrile, containing 0.22 mmol). The reaction mixture was again stirred for a few minutes at 0 C., before being allowed to stir at 25 C. for another 15 hours. It was then concentrated in vacuo, and the residue was subjected to preparative thin layer chromatography on silica gel (Eluent: 1:1 petroleum ether/ethyl acetate) to afford a mixture of diastereomeric products (100 mg). The diastereomers were separated via supercritical fluid chromatography (Column: Chiral Technologies Chiralpak AD, 5 m; Mobile phase: 1:3 ethanol/carbon dioxide). The first-eluting compound was C25, obtained as a light yellow gum. Yield: 50 mg, 94 mol, 31%. LCMS m/z 531.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3), characteristic peaks: 7.28-7.23 (m, 2H, assumed; partially obscured by solvent peak), 6.89 (d, J=8.8 Hz, 2H), 5.53-5.44 (m, 1H), 4.51 (AB quartet, downfield doublet is broadened, J.sub.AB=11.7 Hz, .sub.AB=28 Hz, 2H), 3.97-3.90 (m, 1H), 3.90-3.82 (m, 2H), 3.82 (s, 3H), 3.45-3.36 (m, 1H), 3.28-3.16 (m, 2H), 3.15-3.07 (m, 1H), 2.45-2.36 (m, 1H), 2.36-2.27 (m, 1H), 2.13-2.06 (m, 2H), 2.05-1.93 (m, 2H), 1.87-1.76 (m, 2H), 1.47-1.35 (m, 2H).

(135) The second-eluting diastereomer was C26, also obtained as a light yellow gum. Yield: 50 mg, 94 mol, 31%. LCMS m/z 531.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3), characteristic peaks: 7.28-7.22 (m, 2H, assumed; partially obscured by solvent peak), 6.89 (d, J=8.8 Hz, 2H), 5.54-5.43 (m, 1H), 4.51 (AB quartet, J.sub.AB=12 Hz, .sub.AB=26 Hz, 2H), 3.97-3.90 (m, 1H), 3.90-3.82 (m, 2H), 3.82 (s, 3H), 3.45-3.35 (m, 1H), 3.29-3.16 (m, 2H), 3.16-3.07 (m, 1H), 2.45-2.36 (m, 1H), 2.36-2.28 (m, 1H), 2.14-2.03 (m, 2H), 2.03-1.92 (m, 2H), 1.86-1.75 (m, 2H), 1.46-1.34 (m, 2H).

Step 6. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate [from C25, DIAST-1] (4)

(136) Trifluoroacetic acid (1 mL) was added in a drop-wise manner to a 0 C. solution of C25 (50 mg, 94 mol) in dichloromethane (4 mL), and the reaction mixture was allowed to stir at 0 C. for 1 hour. Saturated aqueous sodium bicarbonate solution (20 mL) was added, and the mixture was extracted with dichloromethane (315 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo; preparative thin layer chromatography on silica gel (Eluent: 1:1 ethyl acetate/petroleum ether) provided the product as a light yellow gum. Yield: 34.5 mg, 84.0 mol, 89%. LCMS m/z 411.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.31-5.18 (br m, 1H), 4.04-3.76 (m, 6H), 3.76-3.66 (m, 2H), 3.44-3.35 (m, 1H), 3.32-3.15 (m, 2H), 3.11 (br dd, J=10, 10 Hz, 1H), 2.68-2.46 (br m, 1H), 2.47-2.28 (m, 2H), 2.28-2.21 (m, 1H), 2.20-1.93 (m, 5H), 1.89-1.75 (m, 2H), 1.65-1.54 (m, 2H), 1.51-1.35 (m, 2H).

Step 7. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate [from C26, DIAST-2] (5)

(137) Compound C26 was converted to the product using the method described for synthesis of 4 from C25. The product was isolated as a yellow gum. Yield: 34.0 mg, 82.8 mol, 88%. LCMS m/z 411.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.30-5.19 (br m, 1H), 4.05-3.77 (m, 6H), 3.77-3.65 (m, 2H), 3.44-3.35 (m, 1H), 3.32-3.17 (m, 2H), 3.12 (br dd, J=10, 10 Hz, 1H), 2.61-2.20 (m, 4H), 2.20-1.94 (m, 5H), 1.90-1.75 (m, 2H), 1.64-1.53 (m, 2H, assumed; partially obscured by water peak), 1.51-1.38 (m, 2H).

Example 6

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (6)

(138) ##STR00048##

Step 1. Synthesis of tert-butyl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C27)

(139) 4-Fluorobenzenesulfonyl chloride (4.18 g, 21.5 mmol) was added portion-wise to a mixture of C23 (5.0 g, 20 mmol), saturated aqueous sodium bicarbonate solution (55 mL), and dichloromethane (195 mL). The reaction mixture was stirred at room temperature overnight, whereupon the aqueous layer was extracted twice with dichloromethane, and the combined organic layers were dried over magnesium sulfate, filtered, and concentrated in vacuo. Silica gel chromatography (Gradient: 0% to 10% methanol in dichloromethane) afforded the product as a white foam. Yield: 8.4 g, 20 mmol, quantitative. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.79-7.73 (m, 2H), 7.28-7.22 (m, 2H, assumed; partially obscured by solvent peak), 3.8-3.66 (m, 2H), 3.79 (dd, J=5.0, 5.0 Hz, 2H), 3.19-3.08 (m, 2H), 3.08-2.89 (m, 2H), 2.89-2.67 (m, 2H), 1.96-1.82 (m, 2H), 1.54-1.48 (m, 2H), 1.47 (s, 9H).

Step 2. Synthesis of 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane, trifluoroacetic acid salt (C28)

(140) Trifluoroacetic acid (15 mL) was slowly added to a solution of C27 (3.16 g, 7.62 mmol) and dichloromethane (38 mL). After the reaction mixture had stirred at room temperature for 2 hours, it was concentrated in vacuo to afford the product, which was used in the next step without further purification. LCMS m/z 315.4 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.81-7.75 (m, 2H), 7.31-7.24 (m, 2H, assumed; partially obscured by solvent peak), 3.81 (br dd, J=5.1, 4.7 Hz, 2H), 3.43-3.34 (m, 2H), 3.33-3.21 (m, 2H), 3.04 (br dd, J=4.9, 4.7 Hz, 2H), 2.86 (s, 2H), 2.24 (br d, J=14.4 Hz, 2H), 1.82 (ddd, J=14.8, 13.3, 4.5 Hz, 2H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C29)

(141) Triethylamine (5.3 mL, 38 mmol) was added to a 0 C. solution of C28 (from the previous step, 7.62 mmol) in in acetonitrile (40 mL). The reaction mixture was allowed to stir at 0 C. for a few minutes, whereupon C2 (reaction solution in acetonitrile, containing 9.9 mmol) was added drop-wise. The temperature was maintained at 0 C. for a few minutes, and then the reaction mixture was allowed to stir at room temperature for 3 days. Solvents were removed in vacuo, and the residue was purified using silica gel chromatography (Gradient: 0% to 50% ethyl acetate in heptane) to afford the product as a white foam. Yield: 3.9 g, 6.6 mmol, 87% over 2 steps. LCMS m/z 635.5 [(M+HCOOH)H+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.79-7.73 (m, 2H), 7.29-7.22 (m, 4H, assumed; partially obscured by solvent peak), 6.96-6.85 (m, 2H), 5.54-5.43 (m, 1H), 4.51 (AB quartet, downfield doublet is broadened, J.sub.AB=11.7 Hz, .sub.AB=28 Hz, 2H), 3.95-3.64 (m, 9H), 3.26-3.13 (m, 2H), 3.08-2.89 (m, 2H), 2.85-2.65 (m, 2H), 2.00-1.87 (m, 2H), 1.55-1.38 (m, 2H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (6)

(142) Trifluoroacetic acid (25 mL) was added drop-wise to a 0 C. solution of C29 (3.9 g, 6.6 mmol) in dichloromethane (100 mL), and the reaction mixture was allowed to warm to room temperature and stir for 2 hours. It was then concentrated in vacuo; the residue was dissolved in ethyl acetate, washed sequentially with saturated aqueous sodium bicarbonate solution and with saturated aqueous sodium chloride solution, dried over magnesium sulfate, filtered, and concentrated under reduced pressure. Silica gel chromatography (Gradient: 0% to 100% ethyl acetate in heptane) provided the product as a white foam. Yield: 2.6 g, 5.5 mmol, 83%. LCMS m/z 471.5 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.85-7.78 (m, 2H), 7.51 (br dd, J=8.9, 8.8 Hz, 2H), 5.30-5.16 (m, 2H), 3.78-3.60 (m, 6H), 3.20-3.02 (m, 2H), 2.94-2.82 (m, 2H), 2.81-2.69 (m, 2H), 1.89-1.75 (m, 2H), 1.57-1.38 (m, 2H).

(143) Crystallization of 6 (1 g) was carried out using ethyl acetate (10 mL) and hexanes (20 mL), providing the product as a white solid, melting point 132 C.; this material was determined to be crystalline via powder X-ray diffraction. Yield for crystallization: 826 mg, 83%. LCMS m/z 471.4 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.85-7.78 (m, 2H), 7.51 (br dd, J=8.8, 8.7 Hz, 2H), 5.30-5.16 (m, 2H), 3.78-3.60 (m, 6H), 3.21-3.01 (m, 2H), 2.95-2.82 (m, 2H), 2.81-2.69 (m, 2H), 1.89-1.75 (m, 2H), 1.58-1.38 (m, 2H).

Example 7

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-(phenylsulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (7)

(144) ##STR00049##

Step 1. Synthesis of 4-tert-butyl 9-{(2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl} 1-oxa-4,9-diazaspiro[5.5]undecane-4,9-dicarboxylate (C30)

(145) Triethylamine (9.28 g, 91.7 mmol) was added to a 0 C. solution of tert-butyl 1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxylate (4.70 g, 18.3 mmol) in acetonitrile (60 mL); C2 (reaction solution in acetonitrile, containing 27.5 mmol) was then added drop-wise, and the reaction mixture was stirred at 0 C. few minutes. It was then allowed to warm to 25 C. and stir for 15 hours, whereupon it was concentrated in vacuo and purified via silica gel chromatography (Gradient: 0% to 100% dichloromethane in petroleum ether). The product (11.2 g) was isolated as a yellow oil, which by LCMS analysis was impure; this material was used without additional purification. LCMS m/z 555.1 [M+Na.sup.+].

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C31)

(146) A mixture of C30 (from the previous step, 4.5 g, 57.4 mmol) and silica gel (5.0 g) was stirred at 150 C. for 3.5 hours, whereupon it was combined with a similar reaction carried out on C30 (4.5 g, 57.4 mmol) and purified via silica gel chromatography (Gradient: 0% to 8% methanol in dichloromethane). The product was obtained as a brown oil. Yield: 2.53 g, 5.85 mmol, 40% over 2 steps. LCMS m/z 433.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.25 (br d, J=8.4 Hz, 2H), 6.88 (br d, J=8.5 Hz, 2H), 5.54-5.43 (br m, 1H), 4.51 (AB quartet, J.sub.AB=11.7 Hz, .sub.AB=27.5 Hz, 2H), 3.95-3.79 (m, 2H), 3.81 (s, 3H), 3.79-3.63 (m, 4H), 3.30-3.14 (m, 2H), 2.86 (dd, J=4.8, 4.5 Hz, 2H), 2.73-2.62 (m, 2H), 2.10-1.91 (m, 2H), 1.50-1.29 (m, 2H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-(phenylsulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C32)

(147) Benzenesulfonyl chloride (61.3 mg, 0.347 mmol) was added to a 5 C. solution of C31 (100 mg, 0.23 mmol) in saturated aqueous sodium bicarbonate solution (2 mL) and dichloromethane (5 mL), and the reaction mixture was stirred at 5 C. for 16 hours. The aqueous layer was extracted with dichloromethane, and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Preparative thin layer chromatography on silica gel (Eluent: 3:1 petroleum ether/ethyl acetate) provided the product as a colorless gum. Yield: 116 mg, 0.203 mmol, 88%. LCMS m/z 594.9 [M+Na.sup.+].

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-(phenylsulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (7)

(148) To a solution of C32 (203 mg, 0.354 mmol) in dichloromethane (10 mL) was added trifluoroacetic acid (2 mL, 30 mmol) and the reaction mixture was stirred at 25 C. for 10 minutes. The reaction was quenched via addition of saturated aqueous sodium bicarbonate solution to a pH of 8, and the resulting mixture was extracted with dichloromethane (220 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Purification via reversed phase HPLC (Column: Phenomenex Luna C18; Mobile phase A: 0.225% formic acid in water; Mobile phase B: acetonitrile; Gradient: 40% to 60% B) afforded the product as a white solid. Yield: 101 mg, 0.224 mmol, 63%. LCMS m/z 452.9 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.78-7.72 (m, 2H), 7.68-7.62 (m, 1H), 7.61-7.55 (m, 2H), 5.32-5.20 (br m, 1H), 4.05-3.95 (br m, 1H), 3.95-3.8 (m, 3H), 3.79 (dd, J=5.1, 4.8 Hz, 2H), 3.32-3.13 (m, 2H), 3.10-2.92 (br m, 2H), 2.90-2.72 (m, 2H), 2.34-2.22 (br m, 1H), 2.04-1.90 (m, 2H), 1.6-1.44 (m, 2H, assumed; partially obscured by water peak).

Example 8 and 9

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (3S)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (8) and (2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (3R)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (9)

(149) ##STR00050##

Step 1. Synthesis of tert-butyl 3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C33)

(150) tert-Butyl 3-amino-1-oxa-8-azaspiro[4.5]decane-8-carboxylate was converted to the product using the method described for synthesis of C32 from C31 in Example 7. The product was isolated as a colorless gum. Yield: 200 mg, 0.504 mmol, 65%. LCMS m/z 296.8 [(M-BOC)+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.91-7.85 (m, 2H), 7.65-7.58 (m, 1H), 7.58-7.51 (m, 2H), 4.82 (br d, J=8 Hz, 1H), 4.00-3.90 (m, 1H), 3.82 (dd, J=9.6, 5.7 Hz, 1H), 3.60-3.48 (m, 3H), 3.31-3.19 (m, 2H), 1.97 (dd, J=13.3, 7.6 Hz, 1H), 1.63-1.48 (m, 5H, assumed; partially obscured by water peak), 1.44 (s, 9H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C34)

(151) Trifluoroacetic acid (2 mL) was added to a solution of C33 (200 mg, 0.504 mmol) in dichloromethane (5 mL), and the reaction mixture was stirred at 25 C. for 1 hour. Removal of solvents in vacuo provided N-(1-oxa-8-azaspiro[4.5]dec-3-yl)benzenesulfonamide, trifluoroacetic acid salt, as a colorless gum, LCMS m/z 297.0 [M+H].sup.+. This material was dissolved in acetonitrile (5 mL), cooled to 0 C., and treated with triethylamine (153 mg, 1.51 mmol). After this solution had stirred at 0 C. for a few minutes, C2 (reaction solution in acetonitrile containing 0.755 mmol) was added drop-wise, and stirring was continued at 0 C. for 30 minutes. The reaction mixture was then allowed to warm to 25 C. and stir for 18 hours, whereupon it was concentrated under reduced pressure. Silica gel chromatography (Gradient: 1% to 34% ethyl acetate in petroleum) afforded the product as a colorless gum. Yield: 180 mg, 0.314 mmol, 62%. LCMS m/z 595.1 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.88 (br d, J=7 Hz, 2H), 7.65-7.60 (m, 1H), 7.59-7.52 (m, 2H), 7.23 (br d, J=8 Hz, 2H), 6.88 (br d, J=8 Hz, 2H), 5.52-5.40 (m, 1H), 4.64-4.58 (m, 1H), 4.50 (AB quartet, J.sub.AB=11.3 Hz, .sub.AB=28 Hz, 2H), 4.01-3.91 (m, 1H), 3.82 (s, 3H), 3.88-3.78 (m, 1H), 3.78-3.62 (m, 4H), 3.59-3.47 (m, 1H), 3.36-3.21 (m, 2H), 2.02-1.91 (m, 1H), 1.72-1.38 (m, 5H, assumed; partially obscured by water peak).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C35)

(152) Trifluoroacetic acid (2 mL) was added to a 0 C. solution of C34 (180 mg, 0.314 mmol) in dichloromethane (8 mL) and the reaction mixture was stirred at 0 C. for 30 minutes, whereupon it was treated with saturated aqueous sodium bicarbonate solution until the pH was above 7. The aqueous layer was extracted with ethyl acetate (55 mL), and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Preparative thin layer chromatography on silica gel (Eluent: 1:1 petroleum ether/ethyl acetate) provided a diastereomeric mixture of the product as a colorless oil. Yield: 130 mg, 0.287 mmol, 91%.

Step 4. Isolation of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3S)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (8) and (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3R)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (9)

(153) Compound C35 (130 mg, 0.287 mmol) was separated into its component diastereomers via supercritical fluid chromatography (Column: Chiral Technologies Chiralpak AD, 5 m; Mobile phase: 3:7 2-propanol/carbon dioxide). The first-eluting diastereomer was further purified by preparative thin layer chromatography on silica gel (Eluent: 1:1 petroleum ether/ethyl acetate) to afford 8 as a colorless gum. Yield for the separation: 62.0 mg, 0.137 mmol, 48%. LCMS m/z 474.8 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.91-7.85 (m, 2H), 7.66-7.59 (m, 1H), 7.59-7.52 (m, 2H), 5.30-5.18 (br m, 1H), 4.89-4.77 (br m, 1H), 4.03-3.90 (m, 2H), 3.90-3.64 (m, 4H), 3.58-3.50 (m, 1H), 3.39-3.19 (m, 2H), 1.99 (dd, J=13.6, 7.6 Hz, 1H), 1.75-1.44 (m, 5H, assumed; partially obscured by water peak).

(154) The second-eluting diastereomer was 9, also isolated as a colorless gum. Yield for the separation: 67.0 mg, 0.148 mmol, 52%. LCMS m/z 475.1 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.91-7.85 (m, 2H), 7.66-7.59 (m, 1H), 7.59-7.52 (m, 2H), 5.29-5.18 (m, 1H), 4.87-4.79 (m, 1H), 4.04-3.90 (m, 2H), 3.90-3.79 (m, 2H), 3.79-3.66 (m, 2H), 3.58-3.50 (m, 1H), 3.41-3.21 (m, 2H), 2.05-1.93 (m, 1H), 1.75-1.39 (m, 5H, assumed; partially obscured by water peak).

(155) The absolute configurations indicated for 8 and 9 were established by relation to the X-ray crystal structure determination of C48 (see Example 15) in the following manner: C48 and its enantiomer C49 were converted to samples of the general structure of 8 and 9 using the methods described in this Example. Supercritical fluid chromatography (Column: Chiral Technologies Chiralpak AD, 5 um; Mobile phase A: carbon dioxide; Mobile phase B: 2-propanol; Gradient: 5% to 60% B) provided a clear correlation between the material derived from C48 and 9 (retention times 7.44 and 7.45 minutes). Likewise, the material derived from C49 exhibited a very similar retention time to that of 8 (6.86 and 6.87 minutes).

Example 10

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-[(5-cyclopropylpyridin-2-yl)oxy]piperidine-1-carboxylate (10)

(156) ##STR00051##

Step 1. Synthesis of tert-butyl 4-[(5-cyclopropylpyridin-2-yl)oxy]piperidine-1-carboxylate (C36)

(157) Potassium tert-butoxide (913 mg, 8.14 mmol) was added to a solution of tert-butyl 4-hydroxypiperidine-1-carboxylate (983 mg, 4.88 mmol) in N,N-dimethylformamide (30 mL) and the reaction mixture was heated at 50 C. for 2 hours. 2-Chloro-5-cyclopropylpyridine (250 mg, 1.63 mmol) was then added, and the reaction mixture was stirred at 100 C. for 18 hours. After solvent had been removed in vacuo, the residue was diluted with water (50 mL) and extracted with ethyl acetate (350 mL); the combined organic layers were concentrated under reduced pressure. Chromatography on silica gel (Gradient: 0% to 10% ethyl acetate in petroleum ether) afforded the product as a white solid. Yield: 120 mg, 0.377 mmol, 23%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.97-7.93 (m, 1H), 7.3-7.21 (m, 1H, assumed; partially obscured by solvent peak), 6.62 (d, J=8.4 Hz, 1H), 5.22-5.13 (m, 1H), 3.82-3.72 (m, 2H), 3.34-3.24 (m, 2H), 2.02-1.92 (m, 2H), 1.88-1.78 (m, 1H), 1.77-1.65 (m, 2H), 1.48 (s, 9H), 0.97-0.90 (m, 2H), 0.65-0.59 (m, 2H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-[(5-cyclopropylpyridin-2-yl)oxy]piperidine-1-carboxylate (C37)

(158) Conversion of C36 to C37 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. The product was isolated as a colorless gum. Yield: 120 mg, 0.243 mmol, 64%.

(159) .sup.1H NMR (400 MHz, CDCl.sub.3) of intermediate 5-cyclopropyl-2-(piperidin-4-yloxy)pyridine, trifluoroacetic acid salt, characteristic peaks: 8.07-8.03 (m, 1H), 7.79 (br d, J=8 Hz, 1H), 7.07 (d, J=9 Hz, 1H), 3.60-3.45 (m, 2H), 3.43-3.32 (m, 2H), 2.46-2.34 (m, 2H), 2.24-2.13 (m, 2H), 2.01-1.91 (m, 1H), 1.17-1.09 (m, 2H), 0.79-0.72 (m, 2H).

(160) Compound C37: LCMS m/z 517.0 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.97-7.92 (m, 1H), 7.30-7.22 (m, 3H, assumed; partially obscured by solvent peak), 6.89 (d, J=8.5 Hz, 2H), 6.64 (d, J=8.4 Hz, 1H), 5.55-5.44 (m, 1H), 5.24-5.15 (m, 1H), 4.52 (AB quartet, J.sub.AB=11.5 Hz, .sub.AB=26.5 Hz, 2H), 3.82 (s, 3H), 3.8-3.66 (m, 4H), 3.52-3.39 (m, 2H), 2.06-1.90 (m, 2H), 1.89-1.70 (m, 3H), 0.98-0.91 (m, 2H), 0.66-0.59 (m, 2H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-[(5-cyclopropylpyridin-2-yl)oxy]piperidine-1-carboxylate (10)

(161) Trifluoroacetic acid (5 mL) was added drop-wise to a solution of C37 (120 mg, 0.243 mmol) in dichloromethane (15 mL), and the reaction mixture was stirred at 30 C. for 2 hours, whereupon it was concentrated in vacuo and diluted with ethyl acetate (20 mL). The resulting mixture was poured into saturated aqueous sodium bicarbonate solution (20 mL) and extracted with ethyl acetate (320 mL); the combined organic layers were concentrated under reduced pressure. Preparative thin layer chromatography on silica gel (Eluent: 1:1 petroleum ether:ethyl acetate) provided the product as a colorless gum. Yield: 70 mg, 0.19 mmol, 78%. LCMS m/z 375.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.94 (d, J=2.1 Hz, 1H), 7.25 (dd, J=8.5, 2.4 Hz, 1H), 6.63 (d, J=8.5 Hz, 1H), 5.32-5.19 (m, 2H), 4.01 (br d, half of AB quartet, J=12 Hz, 1H), 3.88 (dd, half of ABX pattern, J=12, 7 Hz, 1H), 3.87-3.70 (m, 2H), 3.57-3.40 (m, 2H), 2.52-2.40 (br s, 1H), 2.07-1.93 (m, 2H), 1.89-1.74 (m, 3H), 0.98-0.91 (m, 2H), 0.66-0.59 (m, 2H).

Example 11

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-[(3-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (11)

(162) ##STR00052##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-[(3-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C38)

(163) Conversion of C31 to the product was carried out using the method described for synthesis of C32 from C31 in Example 7, providing C38 as a colorless gum. Yield: 130 mg, 0.220 mmol, 79%. LCMS m/z 612.9 [M+Na.sup.+].

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-[(3-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (11)

(164) Trifluoroacetic acid (2 mL, 30 mmol) was added to a solution of C38 (190 mg, 0.322 mmol) in dichloromethane (10 mL) and the reaction mixture was stirred at 25 C. for 10 minutes, whereupon it was treated with saturated aqueous sodium bicarbonate solution to a pH of 8. The mixture was extracted with dichloromethane (220 mL), and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Purification via reversed phase HPLC (Column: Phenomenex Luna C18; Mobile phase A: 0.225% formic acid in water; Mobile phase B: acetonitrile; Gradient: 43% to 63% B) afforded the product as a white solid. Yield: 93.4 mg, 0.198 mmol, 61%. LCMS m/z 470.9 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.61-7.52 (m, 2H), 7.48-7.43 (m, 1H), 7.39-7.32 (m, 1H), 5.31-5.20 (m, 1H), 4.06-3.96 (m, 1H), 3.95-3.83 (m, 3H), 3.80 (dd, J=5.0, 4.9 Hz, 2H), 3.32-3.14 (m, 2H), 3.11-2.95 (m, 2H), 2.91-2.75 (m, 2H), 2.33-2.23 (m, 1H), 2.05-1.92 (m, 2H), 1.6-1.45 (m, 2H, assumed; partially obscured by water peak).

Example 12

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 2-[(4-fluorophenyl)sulfonyl]-2,9-diazaspiro[5.5]undecane-9-carboxylate (12)

(165) ##STR00053##

Step 1. Synthesis of 9-benzyl 2-tert-butyl 2,9-diazaspiro[5.5]undecane-2,9-dicarboxylate (C39)

(166) Saturated aqueous sodium bicarbonate solution (5 mL) and benzyl chloroformate (161 mg, 0.944 mmol) were added to a 0 C. solution of tert-butyl 2,9-diazaspiro[5.5]undecane-2-carboxylate (200 mg, 0.786 mmol) in ethyl acetate (5 mL), and the reaction mixture was stirred for 18 hours at 30 C. The aqueous layer was extracted with ethyl acetate (25 mL), and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo; silica gel chromatography (Gradient: 0% to 20% ethyl acetate in petroleum ether) provided the product as an oil. Yield: 235 mg, 0.605 mmol, 77%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.41-7.29 (m, 5H), 5.13 (s, 2H), 3.73-3.60 (m, 2H), 3.48-3.19 (m, 6H), 1.60-1.50 (m, 2H), 1.50-1.28 (m, 6H), 1.45 (m, 9H).

Step 2. Synthesis of benzyl 2,9-diazaspiro[5.5]undecane-9-carboxylate (C40)

(167) Trifluoroacetic acid (3 mL) was added to a solution of C39 (235 mg, 0.605 mmol) in dichloromethane (5 mL) and the reaction mixture was stirred for 30 minutes at room temperature. After removal of solvents in vacuo, the residue was taken up in aqueous sodium bicarbonate solution (20 mL) and extracted with dichloromethane (320 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo to afford the product as a gum. Yield: 116 mg, 0.402 mmol, 66%. LCMS m/z 289.1 [M+H].sup.+.

Step 3. Synthesis of benzyl 2-[(4-fluorophenyl)sulfonyl]-2,9-diazaspiro[5.5]undecane-9-carboxylate (C41)

(168) 4-Fluorobenzenesulfonyl chloride (117 mg, 0.601 mmol) was added to a solution of C40 (116 mg, 0.402 mmol) in pyridine (2 mL) and the reaction mixture was stirred for 18 hours at 30 C., whereupon it was concentrated in vacuo. The residue was partitioned between dichloromethane (20 mL) and saturated aqueous sodium bicarbonate solution (20 mL), and the organic layer was concentrated under reduced pressure. Silica gel chromatography (Gradient: 0% to 25% ethyl acetate in petroleum ether) provided the product as a gum. Yield: 140 mg, 0.314 mmol, 78%. LCMS m/z 446.9 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.81-7.73 (m, 2H), 7.41-7.29 (m, 5H), 7.22 (dd, J=8.8, 8.4 Hz, 2H), 5.14 (s, 2H), 3.64-3.54 (m, 2H), 3.44-3.32 (m, 2H), 3.22-3.04 (m, 1H), 3.04-2.80 (m, 2H), 2.80-2.60 (m, 1H), 1.77-1.65 (m, 2H), 1.65-1.5 (m, 2H, assumed; obscured by water peak), 1.44 (ddd, J=14, 9, 4 Hz, 2H), 1.39-1.29 (m, 2H).

Step 4. Synthesis of 2-[(4-fluorophenyl)sulfonyl]-2,9-diazaspiro[5.5]undecane (C42)

(169) To a solution of C41 (60.0 mg, 0.134 mmol) in tetrahydrofuran (10 mL) was added 10% palladium on carbon (14.3 mg, 13.4 mol), and the mixture was stirred under a hydrogen atmosphere (45 psi) for 18 hours at 50 C. After filtration of the reaction mixture, the filter cake was washed with methanol (20 mL); the combined filtrates were concentrated in vacuo to afford the product as a colorless gum. Yield: 42.0 mg, 0.134 mmol, 100%. LCMS m/z 312.9 [M+H].sup.+.

Step 5. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 2-[(4-fluorophenyl)sulfonyl]-2,9-diazaspiro[5.5]undecane-9-carboxylate (C43)

(170) Conversion of C42 to the product was effected using the method described for synthesis of C30 in Example 7. In this case, purification was carried out via preparative thin layer chromatography on silica gel (Eluent: 3:1 petroleum ether/ethyl acetate) to afford the product as a gum. Yield: 55 mg, 93 mol, 35%. LCMS m/z 611.0 [M+Na.sup.+].

Step 6. Synthesis of (2R)-1, 1,1-trifluoro-3-hydroxypropan-2-yl 2-[(4-fluorophenyl)sulfonyl]-2,9-diazaspiro[5.5]undecane-9-carboxylate (12)

(171) Conversion of C43 to the product was carried out using the method described for synthesis of 11 from C38 in Example 11, except that the reaction was carried out at 0 C. Purification was effected via preparative thin layer chromatography on silica gel (Eluent: 9:1 dichloromethane/methanol) to provide the product as a white solid. Yield: 13 mg, 28 mol, 30%. LCMS m/z 491.1 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.77 (br dd, J=8.5, 5.0 Hz, 2H), 7.23 (dd, J=8.5, 8.3 Hz, 2H), 5.32-5.20 (m, 1H), 4.05-3.95 (m, 1H), 3.92-3.81 (m, 1H), 3.69-3.53 (m, 2H), 3.50-3.31 (m, 2H), 3.16-3.02 (m, 1H), 3.01-2.84 (m, 2H), 2.81-2.69 (m, 1H), 1.80-1.54 (m, 5H), 1.54-1.42 (m, 2H), 1.41-1.31 (m, 2H).

Example 13

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (3aR,6aS)-5-[(3,4-difluorophenyl)sulfonyl]hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate (13)

(172) ##STR00054##

Step 1. Synthesis of tert-butyl (3aR,6aS)-5-[(3,4-difluorophenyl)sulfonyl]hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate (C44)

(173) tert-Butyl (3aR,6aS)-hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate was converted to C44 using the method described for synthesis of C32 from C31 in Example 7. The product was obtained as a white solid. Yield: 100 mg, 0.257 mmol, 68%. LCMS m/z 410.9 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.68 (ddd, J=9, 7, 2 Hz, 1H), 7.64-7.59 (m, 1H), 7.36 (ddd, J=9, 9, 7 Hz, 1H), 3.57-3.48 (m, 2H), 3.48-3.39 (m, 2H), 3.20-2.98 (m, 4H), 2.89-2.80 (m, 2H), 1.44 (s, 9H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3aR,6aS)-5-[(3,4-difluorophenyl)sulfonyl]hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate (C45)

(174) Conversion of C44 to C45 was effected using the method described for synthesis of C34 from C33 in Examples 8 and 9. .sup.1H NMR (400 MHz, CD.sub.3OD) of intermediate (3aR,6aS)-2-[(3,4-difluorophenyl)sulfonyl]octahydropyrrolo[3,4-c]pyrrole, trifluoroacetic acid salt, 7.80 (ddd, J=9.7, 7.3, 2.2 Hz, 1H), 7.72-7.67 (m, 1H), 7.58 (ddd, J=10.0, 8.7, 7.5 Hz, 1H), 3.60-3.53 (m, 2H), 3.38-3.33 (m, 2H), 3.13-3.07 (m, 2H), 3.07-2.96 (m, 4H). In this case, purification was carried out via preparative thin layer chromatography on silica gel (Eluent: 2:1 petroleum ether/ethyl acetate) to afford C45 as a colorless gum. By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. Yield: 100 mg, 0.18 mmol, 69%. LCMS m/z 587.0 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.71-7.64 (m, 1H), 7.64-7.57 (m, 1H), 7.39-7.31 (m, 1H), 7.28-7.20 (m, 2H, assumed; partially obscured by solvent peak), 6.94-6.85 (m, 2H), 5.47-5.37 (m, 1H), 4.58-4.41 (m, 2H), [3.83 (s) and 3.81 (s), total 3H], 3.77-3.55 (m, 4H), 3.55-3.35 (m, 2H), 3.29-3.05 (m, 4H), 2.95-2.84 (m, 2H).

Step 3. Synthesis of (2R)-1, 1,1-trifluoro-3-hydroxypropan-2-yl (3aR,6aS)-5-[(3,4-difluorophenyl)sulfonyl]hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate (13)

(175) Conversion of C45 to 13 was carried out using the method described for synthesis of C35 from C34 in Examples 8 and 9. The product was isolated as a colorless oil. Yield: 40 mg, 90 mol, 50%. LCMS m/z 445.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.68 (br dd, J=9.0, 7.3 Hz, 1H), 7.64-7.58 (m, 1H), 7.42-7.31 (m, 1H), 5.29-5.18 (m, 1H), 4.03-3.93 (m, 1H), 3.90-3.79 (m, 1H), 3.74-3.58 (m, 2H), 3.52-3.42 (m, 1H), 3.42-3.07 (m, 5H), 2.99-2.84 (m, 2H).

Example 14

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-(5-fluoropyridin-2-yl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (14)

(176) ##STR00055##

Step 1. Synthesis of tert-butyl 4-(5-fluoropyridin-2-yl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C46)

(177) A mixture of C23 (100 mg, 0.39 mmol), 2-chloro-5-fluoropyridine (103 mg, 0.783 mmol), [1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene](3-chloropyridyl)palladium(II) dichloride (26.6 mg, 39.1 mol), and cesium carbonate (381 mg, 1.17 mmol) in toluene (10 mL) was heated at 120 C. for 3 days. The reaction mixture was then filtered and the filtrate was concentrated in vacuo; silica gel chromatography (Gradient: 0% to 10% methanol in dichloromethane) afforded the product as a brown gum. Yield: 135 mg, 0.384 mmol, 98%. LCMS m/z 352.3 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.03 (d, J=3.0 Hz, 1H), 7.26 (ddd, J=9.2, 7.7, 3.1 Hz, 1H), 6.57 (dd, J=9.3, 3.3 Hz, 1H), 3.83 (dd, J=6.0, 4.1 Hz, 2H), 3.8-3.65 (m, 2H), 3.42 (dd, J=5.4, 4.8 Hz, 2H), 3.33 (s, 2H), 3.19 (br dd, J=12, 12 Hz, 2H), 1.91 (br d, J=13 Hz, 2H), 1.56-1.45 (m, 2H), 1.46 (s, 9H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-(5-fluoropyridin-2-yl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C47)

(178) Conversion of C46 to C47 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. LCMS of intermediate 4-(5-fluoropyridin-2-yl)-1-oxa-4,9-diazaspiro[5.5]undecane, bis(trifluoroacetic acid) salt, m/z 252.1 [M+H].sup.+. In this case, purification was carried out using preparative thin layer chromatography (Eluent: 3:1 petroleum ether/ethyl acetate) to afford C47 as a light yellow gum. Yield: 70 mg, 0.13 mmol, 68%. LCMS m/z 528.2 [M+H].sup.+.

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-(5-fluoropyridin-2-yl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (14)

(179) Trifluoroacetic acid (1 mL) was added to a 0 C. solution of C47 (70 mg, 0.13 mmol) in dichloromethane (5 mL), and the reaction mixture was stirred at 25 C. for 1 hour. Solvents were removed in vacuo, and the residue was subjected to preparative thin layer chromatography on silica gel (Eluent: 2:3 petroleum ether/ethyl acetate). Further purification using reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: 0.225% formic acid in water; Mobile phase B: 0.225% formic acid in acetonitrile; Gradient: 38% to 58% B) provided the product as a colorless gum. Yield: 33.4 mg, 82.0 mol, 63%. LCMS m/z 408.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.04 (d, J=2.8 Hz, 1H), 7.31-7.23 (m, 1H, assumed; partially obscured by solvent peak), 6.59 (dd, J=9.2, 3.1 Hz, 1H), 5.32-5.20 (m, 1H), 4.06-3.77 (m, 6H), 3.49-3.39 (m, 2H), 3.39-3.19 (m, 4H), 2.68-2.38 (br s, 1H), 2.08-1.92 (m, 2H), 1.62-1.48 (m, 2H).

Example 15

(2R)-1,1, I-Trifluoro-3-hydroxypropan-2-yl (3R)-3-[methyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (15)

(180) ##STR00056##

Step 1. Synthesis of tert-butyl (3R)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C48) and tert-butyl (3S)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C49)

(181) Reaction of tert-butyl 3-amino-1-oxa-8-azaspiro[4.5]decane-8-carboxylate with benzenesulfonyl chloride was carried out as described for synthesis of C32 from C31 in Example 7. The racemic product was purified using silica gel chromatography (Gradient: 20% to 50% ethyl acetate in heptane) to afford a white solid (2.88 g), which was then separated into its component enantiomers via supercritical fluid chromatography [Column: Phenomenex Lux Cellulose-3, 5 m; Eluent: 7.5% (1:1 methanol/acetonitrile) in carbon dioxide]. The first-eluting product, obtained as a tacky white solid that exhibited a negative () rotation, was designated as C48. Yield: 1.35 g, 3.40 mmol, 45%. LCMS m/z 395.5 [MH+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.90-7.86 (m, 2H), 7.64-7.59 (m, 1H), 7.57-7.52 (m, 2H), 4.81 (d, J=7.9 Hz, 1H), 4.00-3.91 (m, 1H), 3.81 (dd, J=9.7, 5.7 Hz, 1H), 3.59-3.48 (m, 3H), 3.30-3.19 (m, 2H), 1.97 (dd, J=13.4, 7.7 Hz, 1H), 1.67-1.49 (m, 4H), 1.48-1.38 (m, 1H), 1.44 (s, 9H).

(182) The second-eluting product, obtained as a tacky white solid that exhibited a positive (+) rotation, was designated as C49. Yield: 1.15 g, 2.90 mmol, 38%. LCMS m/z 395.5 [MH+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.90-7.86 (m, 2H), 7.64-7.59 (m, 1H), 7.57-7.52 (m, 2H), 4.79 (d, J=8.0 Hz, 1H), 4.00-3.91 (m, 1H), 3.81 (dd, J=9.7, 5.7 Hz, 1H), 3.59-3.48 (m, 3H), 3.30-3.19 (m, 2H), 1.97 (dd, J=13.4, 7.7 Hz, 1H), 1.67-1.49 (m, 4H), 1.47-1.38 (m, 1H), 1.44 (s, 9H).

(183) The absolute configurations shown were established as follows: a portion of this batch of C48 was recrystallized from dichloromethane/tert-butyl methyl ether, and its absolute configuration was determined via single crystal X-ray structure determination:

(184) Single-Crystal X-Ray Structural Determination of C48

(185) Data collection was performed on a Bruker APEX diffractometer at room temperature. Data collection consisted of omega and phi scans.

(186) The structure was solved by direct methods using SHELX software suite in the space group P2.sub.12.sub.12.sub.1. The structure was subsequently refined by the full-matrix least squares method. All non-hydrogen atoms were found and refined using anisotropic displacement parameters.

(187) The hydrogen atom located on nitrogen was found from the Fourier difference map and refined with distances restrained. The remaining hydrogen atoms were placed in calculated positions and were allowed to ride on their carrier atoms. The final refinement included isotropic displacement parameters for all hydrogen atoms.

(188) Analysis of the absolute structure using likelihood methods (Hooft, 2008) was performed using PLATON (Spek, 2010). The results indicate that the absolute structure has been correctly assigned. The method calculates that the probability that the structure is correct is 100.0. The Hooft parameter is reported as 0.015 with an esd of 0.09.

(189) The final R-index was 4.2%. A final difference Fourier revealed no missing or misplaced electron density.

(190) Pertinent crystal, data collection and refinement information is summarized in Table 1. Atomic coordinates, bond lengths, bond angles, and displacement parameters are listed in Tables 2-5.

SOFTWARE AND REFERENCES

(191) SHELXTL, Version 5.1, Bruker AXS, 1997. PLATON, A. L. Spek, J. Appl. Cryst. 2003, 36, 7-13. MERCURY, C. F. Macrae, P. R. Edington, P. McCabe, E. Pidcock, G. P. Shields, R. Taylor, M. Towler, and J. van de Streek, J. Appl. Cryst. 2006, 39, 453-457. OLEX2, O. V. Dolomanov, L. J. Bourhis, R. J. Gildea, J. A. K. Howard, and H. Puschmann, J. Appl. Cryst. 2009, 42, 339-341. R. W. W. Hooft, L. H. Straver, and A. L. Spek, J. Appl. Cryst. 2008, 41, 96-103. H. D. Flack, Acta Cryst. 1983, A39, 867-881.

(192) TABLE-US-00001 TABLE 1 Crystal data and structure refinement for C48. Empirical formula C.sub.19H.sub.28N.sub.2O.sub.5S Formula weight 396.50 Temperature 276(2) K. Wavelength 1.54178 Crystal system Orthorhombic Space group P2.sub.12.sub.12.sub.1 Unit cell dimensions a = 9.79150(10) = 90 b = 11.11580(10) = 90 c = 18.6694(2) = 90 Volume 2031.98(4) .sup.3 Z 4 Density (calculated) 1.296 Mg/m.sup.3 Absorption coefficient 1.686 mm.sup.1 F(000) 848 Crystal size 0.260 0.180 0.140 mm.sup.3 Theta range for data collection 4.630 to 68.568 Index ranges 11 <= h <= 11, 13 <= k <= 13, 20 <= I <= 22 Reflections collected 9404 Independent reflections 3633 [R(int) = 0.0247] Completeness to theta = 70.31 99.3% Absorption correction None Refinement method Full-matrix least-squares on F.sup.2 Data/restraints/parameters 3633/1/251 Goodness-of-fit on F.sup.2 1.067 Final R indices [I > 2sigma(I)] R1 = 0.0418, wR2 = 0.1074 R indices (all data) R1 = 0.0441, wR2 = 0.1098 Absolute structure parameter 0.017(9) Extinction coefficient n/a Largest diff. peak and hole 0.428 and 0.457 e .Math. .sup.3

(193) TABLE-US-00002 TABLE 2 Atomic coordinates (10.sup.4) and equivalent isotropic displacement parameters (.sup.2 10.sup.3) for C48. U(eq) is defined as one-third of the trace of the orthogonalized U.sup.ij tensor. x y z U(eq) S(1) 3733(1) 10920(1) 849(1) 53(1) N(1) 3045(3) 9602(2) 839(2) 59(1) N(2) 3033(2) 7292(2) 1366(2) 52(1) O(1) 5113(3) 10761(2) 1075(1) 74(1) O(2) 2848(3) 11724(2) 1218(1) 68(1) O(3) 29(3) 8787(2) 1780(1) 68(1) O(4) 5295(2) 7383(2) 1100(1) 53(1) O(5) 4386(2) 5806(2) 1709(1) 55(1) C(1) 4868(3) 11071(3) 483(2) 63(1) C(2) 4920(4) 11465(4) 1195(2) 76(1) C(3) 3910(5) 12188(4) 1452(2) 77(1) C(4) 2853(5) 12532(4) 1029(2) 80(1) C(5) 2775(3) 12136(3) 315(2) 64(1) C(6) 3796(3) 11406(2) 54(2) 49(1) C(7) 1575(3) 9468(3) 927(2) 49(1) C(8) 1069(4) 9583(4) 1697(2) 77(1) C(9) 248(3) 8100(3) 1135(2) 48(1) C(10) 1087(3) 8216(3) 724(2) 51(1) C(11) 601(3) 6821(3) 1356(2) 62(1) C(12) 1914(4) 6735(3) 1772(2) 67(1) C(13) 2776(3) 8526(3) 1137(2) 55(1) C(14) 1463(3) 8609(3) 722(2) 49(1) C(15) 4329(3) 6873(2) 1372(2) 46(1) C(16) 5650(3) 5100(3) 1749(2) 50(1) C(17) 6713(4) 5783(4) 2169(2) 69(1) C(18) 6126(5) 4758(4) 1005(2) 82(1) C(19) 5191(4) 3991(3) 2158(2) 62(1)

(194) TABLE-US-00003 TABLE 3 Bond lengths [] and angles [] for C48. S(1)O(2) 1.423(3) S(1)O(1) 1.426(2) S(1)N(1) 1.613(2) S(1)C(6) 1.772(3) N(1)C(7) 1.456(4) N(2)C(15) 1.353(4) N(2)C(13) 1.459(4) N(2)C(12) 1.468(4) O(3)C(8) 1.400(4) O(3)C(9) 1.441(4) O(4)C(15) 1.214(4) O(5)C(15) 1.344(3) O(5)C(16) 1.467(3) C(1)C(6) 1.372(5) C(1)C(2) 1.400(5) C(2)C(3) 1.362(6) C(3)C(4) 1.358(6) C(4)C(5) 1.405(5) C(5)C(6) 1.376(4) C(7)C(10) 1.520(4) C(7)C(8) 1.525(5) C(9)C(11) 1.520(4) C(9)C(10) 1.521(4) C(9)C(14) 1.526(4) C(11)C(12) 1.506(5) C(13)C(14) 1.503(4) C(16)C(17) 1.508(5) C(16)C(18) 1.514(5) C(16)C(19) 1.518(4) O(2)S(1)O(1) 120.73(17) O(2)S(1)N(1) 108.79(15) O(1)S(1)N(1) 106.64(15) O(2)S(1)C(6) 106.86(14) O(1)S(1)C(6) 106.70(15) N(1)S(1)C(6) 106.29(15) C(7)N(1)S(1) 120.3(2) C(15)N(2)C(13) 119.2(2) C(15)N(2)C(12) 123.4(2) C(13)N(2)C(12) 114.8(3) C(8)O(3)C(9) 110.9(2) C(15)O(5)C(16) 122.1(2) C(6)C(1)C(2) 119.8(3) C(3)C(2)C(1) 119.6(4) C(4)C(3)C(2) 120.9(4) C(3)C(4)C(5) 120.4(4) C(6)C(5)C(4) 118.7(3) C(1)C(6)C(5) 120.6(3) C(1)C(6)S(1) 119.9(2) C(5)C(6)S(1) 119.4(3) N(1)C(7)C(10) 112.1(3) N(1)C(7)C(8) 114.8(3) C(10)C(7)C(8) 102.1(3) O(3)C(8)C(7) 107.5(3) O(3)C(9)C(11) 107.7(3) O(3)C(9)C(10) 104.4(2) C(11)C(9)C(10) 114.3(3) O(3)C(9)C(14) 109.9(3) C(11)C(9)C(14) 107.9(2) C(10)C(9)C(14) 112.6(2) C(7)C(10)C(9) 102.8(2) C(12)C(11)C(9) 113.1(3) N(2)C(12)C(11) 110.1(3) N(2)C(13)C(14) 110.9(3) C(13)C(14)C(9) 112.6(2) O(4)C(15)O(5) 125.2(3) O(4)C(15)N(2) 124.5(3) O(5)C(15)N(2) 110.3(2) O(5)C(16)C(17) 109.8(3) O(5)C(16)C(18) 110.3(3) C(17)C(16)C(18) 113.0(3) O(5)C(16)C(19) 102.1(2) C(17)C(16)C(19) 110.6(3) C(18)C(16)C(19) 110.4(3) Symmetry transformations used to generate equivalent atoms.

(195) TABLE-US-00004 TABLE 4 Anisotropic displacement parameters (.sup.2 10.sup.3) for C48. The anisotropic displacement factor exponent takes the form: 2.sup.2[h.sup.2 a*.sup.2U.sup.11 + . . . + 2 h k a* b* U.sup.12]. U.sup.11 U.sup.22 U.sup.33 U.sup.23 U.sup.13 U.sup.12 S(1) 48(1) 42(1) 69(1) 2(1) 10(1) 8(1) N(1) 44(1) 42(1) 91(2) 9(1) 4(1) 3(1) N(2) 41(1) 49(1) 67(2) 17(1) 2(1) 2(1) O(1) 57(1) 69(1) 95(2) 19(1) 28(1) 18(1) O(2) 80(2) 52(1) 70(1) 7(1) 6(1) 9(1) O(3) 66(2) 88(2) 49(1) 8(1) 5(1) 24(1) O(4) 43(1) 49(1) 68(1) 7(1) 4(1) 0(1) O(5) 46(1) 46(1) 73(1) 16(1) 1(1) 4(1) C(1) 45(2) 51(2) 92(2) 0(2) 4(2) 4(1) C(2) 66(2) 78(2) 84(2) 6(2) 20(2) 2(2) C(3) 85(3) 77(2) 69(2) 6(2) 1(2) 2(2) C(4) 77(2) 83(3) 81(2) 12(2) 15(2) 22(2) C(5) 53(2) 65(2) 75(2) 1(2) 2(2) 18(2) C(6) 40(1) 36(1) 70(2) 2(1) 5(1) 4(1) C(7) 42(1) 44(1) 60(2) 2(1) 4(1) 4(1) C(8) 78(2) 83(2) 70(2) 22(2) 9(2) 27(2) C(9) 47(2) 49(2) 48(2) 1(1) 3(1) 6(1) C(10) 46(1) 49(1) 57(2) 5(1) 1(1) 7(1) C(11) 44(2) 54(2) 91(2) 21(2) 9(2) 1(1) C(12) 50(2) 69(2) 83(2) 35(2) 10(2) 9(2) C(13) 48(2) 48(2) 68(2) 10(1) 2(1) 0(1) C(14) 51(2) 45(1) 51(2) 5(1) 1(1) 5(1) C(15) 44(1) 43(1) 50(1) 2(1) 1(1) 2(1) C(16) 51(2) 51(2) 48(2) 5(1) 1(1) 13(1) C(17) 56(2) 80(2) 70(2) 17(2) 7(2) 6(2) C(18) 120(4) 71(2) 56(2) 4(2) 14(2) 37(2) C(19) 71(2) 51(2) 64(2) 12(1) 4(2) 10(2)

(196) TABLE-US-00005 TABLE 5 Hydrogen coordinates (10.sup.4) and isotropic displacement parameters (.sup.2 10.sup.3) for C48. x y z U(eq) H(1X) 3660(30) 8980(20) 932(17) 57(9) H(1) 5558 10584 302 75 H(2) 5639 11234 1490 91 H(3) 3946 12450 1925 92 H(4) 2177 13033 1212 96 H(5) 2047 12362 25 77 H(7) 1107 10063 628 59 H(8A) 776 10401 1791 92 H(8B) 1794 9380 2029 92 H(10A) 938 8151 212 61 H(10B) 1738 7606 872 61 H(11A) 137 6501 1645 75 H(11B) 674 6326 929 75 H(12A) 1811 7141 2229 81 H(12B) 2127 5898 1865 81 H(13A) 3526 8801 840 66 H(13B) 2726 9045 1554 66 H(14A) 1562 8173 275 59 H(14B) 1285 9446 607 59 H(17A) 7038 6448 1888 103 H(17B) 7462 5258 2281 103 H(17C) 6316 6080 2605 103 H(18A) 5376 4423 741 124 H(18B) 6844 4173 1040 124 H(18C) 6460 5461 763 124 H(19A) 4803 4229 2609 93 H(19B) 5962 3476 2242 93 H(19C) 4519 3565 1883 93

Step 2. Synthesis of tert-butyl (3R)-3-[methyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C50)

(197) To a solution of C48 (1.5 g, 3.8 mmol) in N,N-dimethylformamide at 0 C. was added sodium hydride (60% dispersion in mineral oil; 227 mg, 5.67 mmol). The reaction mixture was stirred at room temperature for 30 minutes, whereupon iodomethane (1.61 g, 11.3 mmol) was added, and stirring was continued for 1 hour. Saturated aqueous ammonium chloride solution was added, and the aqueous layer was extracted three times with ethyl acetate. The combined organic layers were dried over magnesium sulfate, filtered, and concentrated in vacuo to provide the product. Yield: 1.53 g, 3.73 mmol, 98%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.80-7.76 (m, 2H), 7.63-7.58 (m, 1H), 7.56-7.50 (m, 2H), 4.73-4.64 (m, 1H), 3.78 (dd, J=10.2, 7.4 Hz, 1H), 3.64-3.51 (m, 2H), 3.55 (dd, J=10.2, 4.9 Hz, 1H), 3.27-3.13 (m, 2H), 2.76 (s, 3H), 1.87 (dd, J=13.5, 9.1 Hz, 1H), 1.63-1.54 (m, 3H), 1.44 (dd, J=13.5, 6.8 Hz, 1H), 1.43 (s, 9H), 1.37 (br ddd, J=13, 10, 4 Hz, 1H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3R)-3-[methyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C51)

(198) Conversion of C50 to C51 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. Purification in this case was effected via silica gel chromatography (Gradient: 0% to 60% ethyl acetate in heptane) to afford the product as a colorless oil. Yield: 1.7 g, 2.9 mmol, 77%. LCMS m/z 609.4 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.82-7.78 (m, 2H), 7.64-7.59 (m, 1H), 7.57-7.52 (m, 2H), 7.23 (br d, J=8.7 Hz, 2H), 6.87 (br d, J=8.6 Hz, 2H), 5.52-5.40 (m, 1H), 4.75-4.63 (m, 1H), 4.49 (AB quartet, upfield doublet is broadened, J.sub.AB=11.7 Hz, .sub.AB=28.4 Hz, 2H), 3.85-3.62 (m, 5H), 3.81 (s, 3H), 3.62-3.52 (m, 1H), 3.34-3.17 (m, 2H), 2.77 (s, 3H), 1.85 (dd, J=13.5, 9.1 Hz, 1H), 1.71-1.53 (m, 3H), 1.46 (dd, J=13.5, 6.9 Hz, 1H), 1.38 (ddd, J=13.5, 11.2, 4.4 Hz, 1H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3R)-3-[methyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (15)

(199) Trifluoroacetic acid (10.8 mL) was added drop-wise to a 0 C. solution of C51 (1.7 g, 2.9 mmol) in dichloromethane (30 mL) and the reaction mixture was stirred for 1.5 hours at room temperature. After removal of solvents in vacuo, the residue was dissolved in ethyl acetate and washed with saturated aqueous sodium bicarbonate solution. The aqueous layer was extracted with ethyl acetate, and the combined organic layers were dried over magnesium sulfate, filtered, and concentrated under reduced pressure. Silica gel chromatography (Gradient: 0% to 80% ethyl acetate in heptane) provided the product as a white solid. Yield: 1.06 g, 2.27 mmol, 78%. LCMS m/z 467.4 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.81-7.77 (m, 2H), 7.64-7.59 (m, 1H), 7.57-7.51 (m, 2H), 5.28-5.18 (m, 1H), 4.74-4.65 (m, 1H), 3.98 (dd, half of ABX pattern, J=12.5, 3.3 Hz, 1H), 3.89-3.69 (m, 3H), 3.80 (dd, J=10.3, 7.4 Hz, 1H), 3.62-3.54 (m, 1H), 3.38-3.19 (m, 2H), 2.77 (s, 3H), 2.4-2.0 (v br s, 1H), 1.94-1.81 (m, 1H), 1.72-1.59 (m, 3H), 1.48 (br dd, J=13, 6 Hz, 1H), 1.45-1.34 (m, 1H).

Example 16

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-hydroxy-4-{[(phenylsulfonyl)amino]methyl}piperidine-1-carboxylate (16)

(200) ##STR00057##

Step 1. Synthesis of tert-butyl 4-hydroxy-4-{[(phenylsulfonyl)amino]methyl}piperidine-1-carboxylate (C52)

(201) tert-Butyl 4-(aminomethyl)-4-hydroxypiperidine-1-carboxylate was converted to C52 using the method described for synthesis of C32 from C31 in Example 7. Purification via preparative thin layer chromatography (Eluent: 10:1 dichloromethane/methanol) afforded the product as a colorless gum. Yield: 127 mg, 0.343 mmol, 79%. LCMS m/z 393.0 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.90-7.84 (m, 2H), 7.64-7.58 (m, 1H), 7.57-7.51 (m, 2H), 5.10 (br t, J=6.6 Hz, 1H), 3.83-3.70 (m, 2H), 3.17 (br dd, J=12, 11 Hz, 2H), 2.92 (br d, J=6 Hz, 2H), 2.16 (br s, 1H), 1.63-1.54 (m, 2H), 1.53-1.45 (m, 2H), 1.45 (s, 9H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-hydroxy-4-{[(phenylsulfonyl)amino]methyl}piperidine-1-carboxylate (C53)

(202) Conversion of C52 to C53 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. Purification in this case was effected via preparative thin layer chromatography on silica gel (Eluent: 1:1 ethyl acetate/petroleum ether) to afford the product as a colorless gum. Yield: 60 mg, 0.11 mmol, 58% over 3 steps. LCMS m/z 569.1 [M+Na.sup.+].

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-hydroxy-4-{[(phenylsulfonyl)amino]methyl}piperidine-1-carboxylate (16)

(203) Conversion of C53 to 16 was carried out using the method described for synthesis of 1 from C12 in Example 1. Purification via reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: 0.1% aqueous hydrochloric acid; Mobile phase B: acetonitrile; Gradient: 28% to 48% B) afforded the product as a white solid. Yield: 23 mg, 54 mol, 49%. LCMS m/z 449.0 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3), characteristic peaks: 7.85 (br d, J=7 Hz, 2H), 7.59 (br dd, half of ABX pattern, J=7, 7 Hz, 1H), 7.53 (br dd, half of ABX pattern, J=7, 7 Hz, 2H), 5.87-5.69 (m, 1H), 5.33-5.20 (m, 1H), 4.02-3.91 (m, 1H), 3.92-3.74 (m, 3H), 3.39-3.16 (m, 2H), 1.74-1.38 (m, 4H).

Example 17

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-(4-fluorobenzyl)-3-oxo-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (17)

(204) ##STR00058##

Step 1. Synthesis of tert-butyl 4-(4-fluorobenzyl)-3-oxo-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C54)

(205) A mixture of C22 (100 mg, 0.370 mmol), 1-(bromomethyl)-4-fluorobenzene (119 mg, 0.629 mmol), and cesium carbonate (241 mg, 0.740 mmol) in N,N-dimethylformamide (2 mL) was stirred at 100 C. for 64 hours. The reaction mixture was then filtered and concentrated in vacuo; the residue was purified by preparative thin layer chromatography on silica gel (Eluent: 1:1 petroleum ether/ethyl acetate), affording the product as a colorless gum. Yield: 42 mg, 0.11 mmol, 30%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.28-7.21 (m, 2H, assumed; partially obscured by solvent peak), 7.04 (br dd, J=8.7, 8.5 Hz, 2H), 4.57 (s, 2H), 4.23 (s, 2H), 3.82-3.67 (m, 2H), 3.14-3.03 (m, 2H), 3.07 (s, 2H), 1.84-1.74 (m, 2H), 1.44 (s, 9H), 1.42-1.32 (m, 2H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-(4-fluorobenzyl)-3-oxo-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C55)

(206) Conversion of C54 to C55 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. Purification was effected via preparative thin layer chromatography on silica gel (Eluent: 1:1 ethyl acetate/petroleum ether) to provide the product as a colorless gum. Yield: 48 mg, 87 mol, 78% over 2 steps. LCMS m/z 577.3 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.27-7.19 (m, 4H, assumed; partially obscured by solvent peak), 7.04 (br dd, J=8.7, 8.4 Hz, 2H), 6.92-6.82 (m, 2H), 5.50-5.40 (m, 1H), 4.64-4.40 (m, 4H), 4.22 (s, 2H), 3.96-3.78 (m, 2H), 3.81 (s, 3H), 3.78-3.63 (m, 2H), 3.24-2.97 (m, 4H), 1.91-1.73 (m, 2H), 1.44-1.28 (m, 2H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-(4-fluorobenzyl)-3-oxo-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (17)

(207) Conversion of C55 to 17 was carried out using the method described for synthesis of C35 from C34 in Examples 8 and 9. Purification via reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: 0.225% formic acid in water; Mobile phase B: 0.225% formic acid in acetonitrile; Gradient: 30% to 50% B) afforded the product as a colorless gum. Yield: 15.4 mg, 35.4 mol, 41%. LCMS m/z 435.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.28-7.21 (m, 2H, assumed; partially obscured by solvent peak), 7.05 (br dd, J=8.5, 8.5 Hz, 2H), 5.29-5.18 (m, 1H), 4.66-4.49 (m, 2H), 4.23 (s, 2H), 4.02-3.95 (m, 1H), 3.93-3.79 (m, 3H), 3.28-3.11 (m, 2H), 3.09 (s, 2H), 1.93-1.80 (m, 2H), 1.46-1.35 (m, 2H).

Example 18

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 2-ethyl-4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (18)

(208) ##STR00059##

Step 1. Synthesis of tert-butyl 4-({[(4-fluorophenyl)sulfonyl]amino}methyl)-4-hydroxypiperidine-1-carboxylate (C56)

(209) 4-Fluorobenzenesulfonyl chloride (2.21 g, 11.4 mmol) was added portion-wise to a 0 C. solution of tert-butyl 4-(aminomethyl)-4-hydroxypiperidine-1-carboxylate (2.95 g, 12.8 mmol) and triethylamine (4.7 mL, 33.7 mmol) in dichloromethane (150 mL) and the reaction mixture was allowed to warm to room temperature and stir for 1 hour. It was then diluted with dichloromethane (100 mL) and washed sequentially with water (200 mL) and with saturated aqueous sodium chloride solution (200 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. The residue was dissolved in ethyl acetate (20 mL); addition of heptane (100 mL) caused a solid to precipitate. Solvents were evaporated off to afford the product as a white solid. Yield: 4.3 g, 11.1 mmol, 97%. LCMS m/z 387.4 [MH+]. .sup.1H NMR (500 MHz, CDCl.sub.3) 7.91-7.86 (m, 2H), 7.22 (br dd, J=8.6, 8.5 Hz, 2H), 5.2-4.9 (v br s, 1H), 3.87-3.67 (m, 2H), 3.24-3.09 (m, 2H), 2.92 (s, 2H), 2.12-1.94 (br s, 1H), 1.63-1.55 (m, 2H), 1.53-1.45 (m, 2H), 1.45 (s, 9H).

Step 2. Synthesis of tert-butyl 2-ethyl-4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C57)

(210) 1,2-Dibromobutane (0.14 mL, 1.2 mmol) was added to a solution of C56 (150 mg, 0.386 mmol) in N,N-dimethylformamide (2 mL). Potassium carbonate (330 mg, 2.4 mmol) was added, and the reaction mixture was heated at 100 C. for 1 hour. It was then cooled to room temperature and treated with additional 1,2-dibromobutane (0.14 mL, 1.2 mmol), followed by potassium carbonate (330 mg, 2.4 mmol). The reaction temperature was increased to 110 C. for 1 hour, whereupon the reaction mixture was partitioned between ethyl acetate (50 mL) and water (50 mL). The organic layer was washed sequentially with water (50 mL) and with saturated aqueous sodium chloride solution (50 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. Silica gel chromatography (Gradient: 0% to 60% ethyl acetate in heptane) provided the product as a colorless, viscous oil. Yield: 115 mg, 0.260 mmol, 67%. LCMS m/z 465.5 [M+Na.sup.+].

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 2-ethyl-4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (18)

(211) Trifluoroacetic acid (0.40 mL, 5.2 mmol) was added to a solution of C57 (115 mg, 0.260 mmol in dichloromethane (5 mL) and the reaction mixture was allowed to stir for 1 hour at room temperature, whereupon it was concentrated in vacuo and mixed with dichloromethane (5 mL) and triethylamine (1.5 mL, 11 mmol). In a separate flask, a solution of C1 (65.0 mg, 0.260 mmol) in tetrahydrofuran (2 mL) was treated sequentially with bis(pentafluorophenyl) carbonate (102 mg, 0.259 mmol) and triethylamine (1.8 mL, 13 mmol), and this reaction was allowed to stir at room temperature for 1 hour. The solution containing the deprotected C57 was added to the carbonate reaction mixture, and stirring was continued for 2 hours at room temperature. The reaction mixture was then partitioned between ethyl acetate (100 mL) and saturated aqueous sodium bicarbonate solution (60 mL), and the organic layer was washed with aqueous sodium hydrogen sulfate solution (1 M, 60 mL), dried over sodium sulfate, filtered, and concentrated under reduced pressure. The resulting viscous oil was taken up in dichloromethane (5 mL); trifluoroacetic acid (2 mL) was added at room temperature while the reaction mixture was stirred. The reaction mixture was allowed to stir for an additional 30 minutes, whereupon it was concentrated in vacuo; the residue was dissolved in dichloromethane (5 mL) and concentrated once more. Purification was carried out via reversed phase HPLC (Column: Waters Sunfire C18, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 35% to 55% B) to provide the product. Yield: 12.3 mg, 24.7 mol, 10%. LCMS m/z 499.2 [M+H].sup.+. Retention time: 2.79 minutes [Analytical HPLC column: Waters Atlantis dC18, 4.650 mm, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 5.0% to 95% B, linear over 4.0 minutes; Flow rate: 2 mL/minute].

Examples 19, 20 and 21

(212) 1,1,1,3,3-Pentafluoro-4-hydroxybutan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (19); 1,1,1,3,3-Pentafluoro-4-hydroxybutan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate, ENT-1 (20); and 1,1,1,3,3-Pentafluoro-4-hydroxybutan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate, ENT-2 (21)

(213) ##STR00060##

Step 1. Synthesis of 2,2,4,4,4-pentafluorobutane-1,3-diol (C58)

(214) n-Butyllithium (2.5 M solution in hexanes; 23.9 mL, 59.8 mmol) was added drop-wise to a 78 C. solution of 1,1,1,3,3,3-hexafluoropropan-2-ol (4.90 g, 29.2 mmol) in tetrahydrofuran (40 mL). The reaction mixture was stirred for 10 minutes at 78 C., then allowed to warm to 0 C. and stir for 1 hour. Paraformaldehyde (8.7 g, 0.29 mol) was added in a portion-wise manner, and the reaction mixture was stirred at room temperature overnight. Water (50 mL) was added, followed by sodium borohydride (3.7 g, 98 mmol) {Caution: exothermic reaction, accompanied by gas evolution!)}; in the course of the addition, the reaction mixture was cooled in an ice bath to control the reaction. Upon completion of the addition, stirring was continued overnight at room temperature, whereupon the reaction was quenched via addition of 1 M aqueous hydrochloric acid (Caution: gas evolution). The resulting mixture was extracted with ethyl acetate, and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo to afford the product as a yellow-brown oil. Yield: 4.5 g, 25 mmol, 86%. .sup.1H NMR (400 MHz, CDCl.sub.3) 4.50-4.38 (m, 1H), 4.14-4.02 (m, 1H), 4.00-3.89 (m, 1H).

Step 2. Synthesis of 4-{[tert-butyl(dimethyl)silyl]oxy}-1,1,1,3,3-pentafluorobutan-2-ol (C59)

(215) N,N-Dimethylformamide (5 mL) was added to a 0 C. solution of C58 (6.30 g, 35.0 mmol) and 1H-imidazole (2.62 g, 38.5 mmol) in dichloromethane (60 mL). tert-Butyl(dimethyl)silyl chloride (5.27 g, 35.0 mmol) was then introduced portion-wise, and the reaction mixture was allowed to warm to room temperature and stir for 4 days. Saturated aqueous ammonium chloride solution (100 mL) was added, and the aqueous layer was extracted with dichloromethane (230 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo; silica gel chromatography (Eluent: 5% ethyl acetate in petroleum ether) afforded the product as a yellow oil. Yield: 3.0 g, 10 mmol, 29%. .sup.1H NMR (400 MHz, CDCl.sub.3) 4.44-4.31 (m, 1H), 4.13-4.01 (m, 1H), 3.95-3.85 (m, 1H), 3.57-3.46 (m, 1H), 0.92 (s, 9H), 0.13 (s, 6H).

Step 3. Synthesis of 4-{[tert-butyl(dimethyl)silyl]oxy}-1,1,1,3,3-pentafluorobutan-2-yl pentafluorophenyl carbonate (C60)

(216) Bis(pentafluorophenyl) carbonate (158 mg, 0.401 mmol) was added to a 0 C. solution of C59 (118 mg, 0.401 mmol) in acetonitrile (4 mL). Triethylamine (122 mg, 1.20 mmol) was added drop-wise to the reaction mixture, which was stirred briefly in the ice bath, and then allowed to warm to 25 C. and stir for 2 hours. The reaction solution of C60 was used directly in the following step.

Step 4. Synthesis of 4-{[tert-butyl(dimethyl)silyl]oxy}-1,1,1,3,3-pentafluorobutan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C61)

(217) Triethylamine (118 mg, 1.17 mmol) was added to a 0 C. solution of C28 (100 mg, 0.233 mmol) in acetonitrile (5 mL). After a few minutes, C60 (reaction solution from the previous step; 0.401 mmol) was added drop-wise to the 0 C. mixture, which was stirred in the ice bath for several minutes, stirred at 28 C. for 20 hours, and then cooled to 0 C. A second batch of C60 (using the same scale and method as step 3 above; 0.401 mmol) was prepared and added to the 0 C. reaction mixture, which was allowed to warm to room temperature and stir overnight. After removal of volatiles in vacuo, the residue was purified using preparative thin layer chromatography on silica gel (Eluent: 3:1 petroleum ether/ethyl acetate) to afford the product as a white solid. Yield: 120 mg, 0.189 mmol, 81%. By .sup.1H NMR analysis, this material was judged to be a mixture of rotamers. .sup.1H NMR (400 MHz, CD.sub.3OD) 7.87-7.80 (m, 2H), 7.36 (br dd, J=8.7, 8.7 Hz, 2H), 5.84-5.70 (m, 1H), 4.63-4.54 (m, 1H), 3.97-3.77 (m, 5H), 3.3-3.15 (m, 2H, assumed; partially obscured by solvent peak), 3.02-2.95 (m, 2H), 2.86-2.78 (m, 2H), 2.06-1.94 (m, 2H), 1.61-1.45 (m, 2H), [0.93 (s) and 0.90 (s), total 9H], [0.12 (s), 0.10 (s), and 0.08 (s), total 6H].

Step 5. Synthesis of 1,1,1,3,3-pentafluoro-4-hydroxybutan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (19)

(218) Trifluoroacetic acid (4 mL) and water (1 mL) were added drop-wise to a 0 C. solution of C61 (120 mg, 0.189 mmol) in dichloromethane (6 mL) and the reaction mixture was stirred at 28 C. for 3 hours. It was then concentrated in vacuo and partitioned between ethyl acetate (50 mL) and saturated aqueous sodium bicarbonate solution (50 mL); the organic layer was washed with saturated aqueous sodium bicarbonate solution (320 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. Preparative thin layer chromatography on silica gel (Eluent: 1:1 petroleum ether/ethyl acetate) provided the product as a colorless gum. Yield: 73 mg, 0.14 mmol, 74%. LCMS m/z 521.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD) 7.84 (br dd, J=8.8, 5.0 Hz, 2H), 7.37 (br dd, J=8.8, 8.7 Hz, 2H), 5.86-5.73 (m, 1H), 3.91-3.72 (m, 6H), 3.3-3.17 (m, 2H, assumed; partially obscured by solvent peak), 3.01-2.94 (m, 2H), 2.86-2.76 (m, 2H), 2.02-1.92 (m, 2H), 1.60-1.47 (m, 2H).

Step 6. Isolation of 1,1,1,3,3-pentafluoro-4-hydroxybutan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate, ENT-1 (20), and 1,1,1,3,3-pentafluoro-4-hydroxybutan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate, ENT-2 (21)

(219) The racemate 19 was separated into its component enantiomers via supercritical fluid chromatography [Column: Chiral Technologies Chiralcel OD, 3 m; Gradient: 5% to 40% (2-propanol containing 0.05% diethylamine) in carbon dioxide]. The first-eluting enantiomer was 20, obtained as a colorless gum. Yield: 19.9 mg, 38.2 mol, 27% for the separation. LCMS m/z 521.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD) 7.84 (br dd, J=8.7, 5.1 Hz, 2H), 7.37 (br dd, J=8.8, 8.7 Hz, 2H), 5.86-5.73 (m, 1H), 3.91-3.72 (m, 6H), 3.3-3.17 (m, 2H, assumed; partially obscured by solvent peak), 3.01-2.94 (m, 2H), 2.86-2.76 (m, 2H), 2.03-1.92 (m, 2H), 1.61-1.47 (m, 2H). Retention time via supercritical fluid chromatography: 3.97 minutes (Column: Chiral Technologies Chiralcel OD-3, 4.6 mm150 mm I.D., 3 m; Mobile phase A: carbon dioxide; Mobile phase B: 2-propanol containing 0.05% diethylamine; Gradient: 5% to 40% B; Flow rate: 2.5 mL/minute).

(220) The second-eluting enantiomer was 21, also isolated as a colorless gum. Yield: 19.6 mg, 37.6 mol, 27% for the separation. LCMS m/z 521.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD) 7.87-7.81 (m, 2H), 7.41-7.33 (m, 2H), 5.85-5.73 (m, 1H), 3.91-3.72 (m, 6H), 3.3-3.17 (m, 2H, assumed; partially obscured by solvent peak), 3.01-2.94 (m, 2H), 2.86-2.76 (m, 2H), 2.03-1.92 (m, 2H), 1.60-1.47 (m, 2H). Retention time via supercritical fluid chromatography: 4.38 min (Same analytical conditions as those described for 20).

Example 22

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-(morpholin-4-ylsulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (22)

(221) ##STR00061##

Step 1. Synthesis of tert-butyl 4-(morpholin-4-ylsulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C62)

(222) Reaction of C23 with morpholine-4-sulfonyl chloride was carried out using the method described for synthesis of C32 from C31 in Example 7, providing the product as a colorless gum. Yield: 100 mg, 0.247 mmol, 63%. LCMS m/z 428.2 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 3.81-3.70 (m, 8H), 3.29-3.21 (m, 6H), 3.15 (br dd, J=12, 12 Hz, 2H), 3.06 (s, 2H), 1.95-1.86 (m, 2H), 1.52-1.41 (m, 2H), 1.46 (s, 9H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-(morpholin-4-ylsulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C63)

(223) Conversion of C62 to C63 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. LCMS of intermediate 4-(morpholin-4-ylsulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane, trifluoroacetic acid salt: m/z 306.0 [M+H].sup.+. In this case, purification was carried out using preparative thin layer chromatography (Eluent: 1:1 petroleum ether/ethyl acetate) to afford C63 as a colorless gum. Yield: 90.0 mg, 0.155 mmol, 65%. LCMS m/z 603.9 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.23 (d, J=8.5 Hz, 2H), 6.87 (d, J=8.4 Hz, 2H), 5.52-5.41 (m, 1H), 4.50 (AB quartet, J.sub.AB=11.7 Hz, .sub.AB=28.2 Hz, 2H), 3.95-3.80 (m, 2H), 3.80 (s, 3H), 3.78-3.64 (m, 8H), 3.28-3.16 (m, 8H), 3.07-3.00 (m, 2H), 1.99-1.90 (m, 2H), 1.50-1.40 (m, 2H).

Step 3. Synthesis of (2R)-1, 1,1-trifluoro-3-hydroxypropan-2-yl 4-(morpholin-4-ylsulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (22)

(224) Conversion of C63 to 22 was carried out using the method described for synthesis of C35 from C34 in Examples 8 and 9. Purification via reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: 0.225% formic acid in water; Mobile phase B: 0.225% formic acid in acetonitrile; Gradient: 25% to 45% B) afforded the product as a colorless gum. Yield: 33.4 mg, 72.3 mol, 47%. LCMS m/z 462.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.32-5.21 (m, 1H), 4.06-3.96 (m, 1H), 3.96-3.82 (m, 3H), 3.82-3.69 (m, 6H), 3.34-3.18 (m, 8H), 3.07 (s, 2H), 2.34-2.21 (m, 1H), 2.06-1.95 (m, 2H), 1.6-1.42 (m, 2H, assumed; partially obscured by water peak).

Example 23

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-(4-fluorobenzyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (23)

(225) ##STR00062##

Step 1. Synthesis of tert-butyl 3-(4-fluorobenzyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (C64)

(226) A solution of 1-(bromomethyl)-4-fluorobenzene (134 mg, 0.709 mmol) in acetonitrile (3 mL) was slowly added to a room temperature mixture of tert-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate (150 mg, 0.706 mmol) and potassium carbonate (293 mg, 2.12 mmol) in acetonitrile (12 mL) and the reaction mixture was stirred at 25 C. for 16 hours. It was then filtered, and the filtrate was concentrated in vacuo; silica gel chromatography (Gradient: 0% to 20% ethyl acetate in petroleum ether) afforded the product as a colorless gum. Yield: 226 mg, 0.705 mmol, quantitative. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.30-7.24 (m, 2H), 6.99 (br dd, J=8.8, 8.7 Hz, 2H), 4.26-4.03 (m, 2H), 3.43 (s, 2H), 2.58 (dd, J=10.7, 2.3 Hz, 2H), 2.36-2.16 (m, 2H), 1.93-1.78 (m, 4H), 1.47 (s, 9H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-(4-fluorobenzyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (C65)

(227) Conversion of C64 to C65 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. LCMS of intermediate 3-(4-fluorobenzyl)-3,8-diazabicyclo[3.2.1]octane, bis(trifluoroacetic acid) salt: m/z 221.1 [M+H].sup.+. In this case, purification was carried out via silica gel chromatography (Gradient: 0% to 5% methanol in dichloromethane) to afford C65 as a colorless gum. Yield: 150 mg, 0.302 mmol, 88% over 2 steps. LCMS m/z 497.2 [M+H].sup.+.

Step 3. Synthesis of (2R)-1, 1,1-trifluoro-3-hydroxypropan-2-yl 3-(4-fluorobenzyl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (23)

(228) Conversion of C65 to 23 was carried out using the method described for synthesis of 7 from C32 in Example 7. In this case, purification was effected via reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: 0.225% formic acid in water; Mobile phase B: 0.225% formic acid in acetonitrile; Gradient: 10% to 30% B) to provide the product as a colorless gum. Yield: 75 mg, 0.199 mmol, 66%. LCMS m/z 377.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.31-7.23 (m, 2H, assumed; partially obscured by solvent peak), 7.01 (br dd, J=8.8, 8.7 Hz, 2H), 5.33-5.22 (m, 1H), 4.31-4.21 (m, 2H), 4.06-3.96 (m, 1H), 3.93-3.83 (m, 1H), 3.49-3.43 (m, 2H), 2.69-2.61 (m, 2H), 2.38-2.20 (m, 3H), 2.00-1.84 (m, 4H).

Example 24

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-hydroxy-4-{[methyl(phenylsulfonyl)amino]methyl}piperidine-1-carboxylate (24)

(229) ##STR00063##

Step 1. Synthesis of tert-butyl 4-hydroxy-4-[(methylamino)methyl]piperidine-1-carboxylate (C66)

(230) Methylamine (2 M solution in tetrahydrofuran; 0.245 mL, 0.490 mmol) was added to a solution of tert-butyl 1-oxa-6-azaspiro[2.5]octane-6-carboxylate (95 mg, 0.44 mmol) in ethanol (2 mL) and the reaction mixture was heated to 80 C. for 20 hours. Concentration in vacuo provided the product as an oil (105 mg); this material was used in the following step without additional purification.

Step 2. Synthesis of tert-butyl 4-hydroxy-4-{[methyl(phenylsulfonyl)amino]methyl}piperidine-1-carboxylate (C67)

(231) To a solution of C66 (from the previous step; 105 mg, 50.44 mmol) in acetonitrile (2 mL) were added benzenesulfonyl chloride (0.110 mL, 0.862 mmol) and potassium carbonate (119 mg, 0.861 mmol). The reaction mixture was stirred at 25 C. for 3 hours, whereupon it was concentrated in vacuo; silica gel chromatography (Eluent: ethyl acetate) afforded the product as a gum. Yield: 115 mg, 0.299 mmol, 68% over 2 steps. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.82-7.77 (m, 2H), 7.65-7.59 (m, 1H), 7.58-7.52 (m, 2H), 3.95-3.81 (m, 2H), 3.24-3.10 (m, 2H), 3.04-2.91 (m, 2H), 2.90 (s, 3H), 1.70-1.61 (m, 2H), 1.56-1.46 (m, 2H), 1.45 (s, 9H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-hydroxy-4-{[methyl(phenylsulfonyl)amino]methyl}piperidine-1-carboxylate (C68)

(232) Conversion of C67 to C68 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. LCMS of intermediate N-[(4-hydroxypiperidin-4-yl)methyl]-N-methylbenzenesulfonamide, trifluoroacetic acid salt: m/z 285.0 [M+H].sup.+. In this case, purification was carried out via silica gel chromatography (Gradient: 40% to 60% ethyl acetate in petroleum ether), affording C68 as a colorless gum. By .sup.1H NMR analysis, this was judged to be a mixture of diastereomers. Yield: 130 mg, 0.232 mmol, 78% over 2 steps. LCMS m/z 583.1 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.81 (br d, J=8 Hz, 2H), 7.67-7.62 (m, 1H), 7.61-7.54 (m, 2H), 7.25 (d, J=8.5 Hz, 2H), 6.92-6.84 (m, 2H), 5.55-5.43 (m, 1H), 4.51 (AB quartet, upfield doublet is broadened, J.sub.AB=11.7 Hz, .sub.AB=29 Hz, 2H), 4.07-3.90 (m, 2H), 3.85-3.65 (m, 2H), [3.82 (s) and 3.77 (s), total 3H], 3.37-3.22 (m, 2H), 3.01-2.79 (m, 2H), [2.91 (s) and 2.87 (s), total 3H], 1.75-1.64 (m, 2H), 1.55-1.43 (m, 2H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-hydroxy-4-{[methyl(phenylsulfonyl)amino]methyl}piperidine-1-carboxylate (24)

(233) Trifluoroacetic acid (1.2 mL, 16 mmol) was added drop-wise to a 0 C. solution of C68 (130 mg, 0.232 mmol) in acetonitrile (5 mL). The reaction mixture was stirred at room temperature for 30 minutes, whereupon saturated aqueous sodium bicarbonate solution was added until the mixture reached a pH of approximately 8. The organic layer was dried over sodium sulfate, filtered, and concentrated in vacuo to provide an off-white solid; purification via reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: 0.225% formic acid in water; Mobile phase B: acetonitrile; Gradient: 30% to 50% B) afforded the product. Yield: 51.6 mg, 0.117 mmol, 50%. LCMS m/z 463.1 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.83-7.77 (m, 2H), 7.67-7.61 (m, 1H), 7.60-7.53 (m, 2H), 5.31-5.21 (m, 1H), 4.05-3.92 (m, 3H), 3.90-3.81 (m, 1H), 3.41-3.22 (m, 2H), 3.04-2.93 (m, 2H), 2.90 (s, 3H), 2.84-2.74 (br s, 1H), 1.77-1.67 (m, 2H), 1.64-1.46 (m, 2H).

Example 25

(2R)-1, 1,1-Trifluoro-3-hydroxypropan-2-yl 4-(4-fluorobenzyl)piperazine-1-carboxylate (25)

(234) ##STR00064##

Step 1. Synthesis of tert-butyl 4-(4-fluorobenzyl)piperazine-1-carboxylate (C69)

(235) To a 30 C. solution of tert-butyl piperazine-1-carboxylate (200 mg, 1.07 mmol) and potassium carbonate (445 mg, 3.22 mmol) in acetonitrile (8 mL) was added a solution of 1-(bromomethyl)-4-fluorobenzene (203 mg, 1.07 mmol) in acetonitrile (2 mL), in a drop-wise manner. The reaction mixture was stirred for 16 hours at 30 C., whereupon it was concentrated in vacuo and purified via chromatography on silica gel (Gradient: 0% to 20% ethyl acetate in petroleum ether) to afford the product as a colorless gum. Yield: 250 mg, 0.849 mmol, 79%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.28 (br dd, J=8.2, 5.5 Hz, 2H), 7.01 (br dd, J=8.8, 8.7 Hz, 2H), 3.47 (s, 2H), 3.43 (br dd, J=5, 5 Hz, 4H), 2.37 (br dd, J=5, 5 Hz, 4H), 1.46 (s, 9H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-(4-fluorobenzyl)piperazine-1-carboxylate (C70)

(236) Conversion of C69 to C70 was carried out using the method described for synthesis of C34 from C33 in Examples 8 and 9. In this case, purification was carried out using preparative thin layer chromatography (Eluent: 3:1 petroleum ether/ethyl acetate) to afford the product as a colorless gum. Yield: 71 mg, 0.15 mmol, 74% over 2 steps. LCMS m/z 471.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.31-7.22 (m, 4H), 7.01 (br dd, J=8.8, 8.7 Hz, 2H), 6.88 (br d, J=8.8 Hz, 2H), 5.53-5.43 (m, 1H), 4.51 (AB quartet, J.sub.AB=11.7 Hz, .sub.AB=27.9 Hz, 2H), 3.81 (s, 3H), 3.76 (dd, half of ABX pattern, J=11.1, 4.0 Hz, 1H), 3.69 (dd, half of ABX pattern, J=11.2, 7.0 Hz, 1H), 3.60-3.45 (m, 4H), 3.50 (s, 2H), 2.51-2.36 (m, 4H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-(4-fluorobenzyl)piperazine-1-carboxylate (25)

(237) Trifluoroacetic acid (1 mL) was added to a 0 C. solution of C70 (61 mg, 0.13 mmol) in dichloromethane (4 mL). The reaction mixture was stirred at 25 C. for 1 hour, whereupon it was basified to pH 7 via addition of saturated aqueous sodium bicarbonate solution, and extracted with dichloromethane (210 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Preparative thin layer chromatography (Eluent: 10:1 dichloromethane/methanol) provided the product as a colorless gum. Yield: 24.2 mg, 69.1 mol, 53%. LCMS m/z 351.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.28 (br dd, J=8.2, 5.6 Hz, 2H), 7.02 (br dd, J=8.7, 8.7 Hz, 2H), 5.30-5.20 (m, 1H), 4.00 (br dd, half of ABX pattern, J=12, 3 Hz, 1H), 3.86 (dd, half of ABX pattern, J=12.4, 6.8 Hz, 1H), 3.63-3.43 (m, 4H), 3.49 (s, 2H), 2.52-2.34 (m, 4H).

Example 26

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-(isoquinolin-1-yloxy)piperidine-1-carboxylate, trifluoroacetic acid salt (26)

(238) ##STR00065##

(239) A solution of tert-butyl 4-hydroxypiperidine-1-carboxylate (30.2 mg, 0.15 mmol) in N,N-dimethylformamide (0.5 mL) was added to 1-chloroisoquinoline (24.5 mg, 0.15 mmol) in a reaction vial. Potassium tert-butoxide (1 M solution in tetrahydrofuran; 0.45 mL, 0.45 mmol) was added, and the reaction mixture was shaken at 60 C. for 18 hours, then at 100 C. for 1 hour. It was then partitioned between half-saturated aqueous sodium bicarbonate solution (1.5 mL) and ethyl acetate (2.4 mL) and subjected to vortexing, followed by centrifugation to break up an emulsion. The organic layer was eluted through a solid phase extraction cartridge (6 mL) charged with sodium sulfate (1 g); this extraction procedure was repeated twice, and the combined eluents were concentrated in vacuo. A mixture of trifluoroacetic acid and 1,2-dichloroethane (1:1, 1 mL) was added, and the reaction mixture was shaken at room temperature for 2.5 hours, whereupon it was concentrated in vacuo and dissolved in 1,2-dichloroethane (2.4 mL) with vortexing. This material was loaded onto an SCX (strong cation exchanger) solid phase extraction cartridge (Silicycle, 6 mL, 1 g); the vial was rinsed with a mixture of methanol and 1,2-dichloroethane (1:1; 22.4 mL). The cartridge was eluted with methanol (5 mL), followed by a solution of triethylamine in methanol (1 M, 7.5 mL) to elute the deprotected intermediate. Fractions containing the desired material were concentrated in vacuo, and the residue was azeotroped with toluene (21 mL) to remove trace methanol. The residue was dissolved in dichloromethane (0.5 mL).

(240) A crude solution of C2 was prepared separately, as follows: Bis(pentafluorophenyl) carbonate (1.89 g, 4.80 mmol) and triethylamine (13.4 ml, 96.1 mmol) were added to a stirring solution of C1 (1.23 g, 4.91 mmol) in tetrahydrofuran (15 mL). Sufficient tetrahydrofuran was added to bring the total volume to 32 mL, and the reaction mixture was stirred at room temperature for 1 hour. A portion of this crude C2 solution (1.0 mL, 0.15 mmol of C2 and 3 mmol of triethylamine) was added to the deprotected amine solution prepared above, and the reaction mixture was shaken at room temperature overnight. It was then partitioned between half-saturated aqueous sodium bicarbonate solution (1.5 mL) and ethyl acetate (2.4 mL) and subjected to vortexing. The organic layer was eluted through a solid phase extraction cartridge (6 mL) charged with sodium sulfate (1 g); this extraction procedure was repeated twice, and the combined eluents were concentrated in vacuo. This material was treated with a mixture of trifluoroacetic acid and 1,2-dichloroethane (1:1, 1 mL) and shaken at room temperature for 1 hour, whereupon it was concentrated in vacuo and purified using reversed phase HPLC (Column: Waters Sunfire C18, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 20% to 100% B) to afford the product. Yield: 2.5 mg, 6.5 mol, 4%. LCMS m/z 385.1 [M+H].sup.+. Retention time 3.01 minutes [Analytical HPLC conditionsColumn: Waters Atlantis dC18, 4.650 mm, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 5.0% to 95% B, linear over 4.0 minutes; Flow rate: 2 mL/minute].

Example 27

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-(pyridin-2-ylamino)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (27)

(241) ##STR00066## ##STR00067##

Step 1. Synthesis of tert-butyl 3-{[(prop-2-en-1-yloxy)carbonyl]amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C71)

(242) Prop-2-en-1-yl carbonochloridate (9.87 g, 81.9 mmol) was added to a 0 C. solution of tert-butyl 3-amino-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (14.0 g, 54.6 mmol) in saturated aqueous sodium bicarbonate solution (400 mL) and tetrahydrofuran (100 mL). The reaction mixture was stirred at 22 C. for 16 hours, whereupon it was filtered and the filter cake was washed with ethyl acetate. The aqueous layer from the combined filtrates was extracted with ethyl acetate (2200 mL), and the combined organic layers were washed with saturated ammonium chloride solution (3100 mL), dried over sodium sulfate, filtered, and concentrated in vacuo to provide the product as a yellow oil, which solidified upon standing at room temperature. Yield: 18.3 g, 53.8 mmol, 98%. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.98-5.85 (m, 1H), 5.34-5.27 (m, 1H), 5.26-5.20 (m, 1H), 4.95-4.86 (m, 1H), 4.56 (br d, J=4.6 Hz, 2H), 4.38-4.28 (m, 1H), 4.00 (dd, J=9.5, 5.6 Hz, 1H), 3.67 (br dd, J=9.7, 4.0 Hz, 1H), 3.66-3.52 (m, 2H), 3.37-3.24 (m, 2H), 2.13 (dd, J=13.3, 7.6 Hz, 1H), 1.72-1.49 (m, 5H, assumed; partially obscured by water peak), 1.46 (s, 9H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-{[(prop-2-en-1-yloxy)carbonyl]amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C72)

(243) Conversion of C71 to C72 was effected using the method described for synthesis of C34 from C33 in Examples 8 and 9. The product was isolated as a light yellow oil. Yield: 12.6 g, 24.2 mmol, 89% over 2 steps. LCMS m/z 539.1 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.24 (br d, J=8.5 Hz, 2H), 6.88 (br d, J=8.7 Hz, 2H), 5.98-5.85 (m, 1H), 5.53-5.41 (m, 1H), 5.35-5.26 (m, 1H), 5.26-5.19 (m, 1H), 5.00-4.89 (m, 1H), 4.62-4.50 (m, 3H), 4.46 (d, half of AB quartet, J=11.7 Hz, 1H), 4.38-4.26 (m, 1H), 4.04-3.96 (m, 1H), 3.85-3.62 (m, 4H), 3.81 (s, 3H), 3.41-3.25 (m, 2H), 2.19-2.06 (m, 1H), 1.78-1.46 (m, 5H, assumed; partially obscured by water peak).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-amino-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C73)

(244) Palladium(II) acetate (520 mg, 2.32 mmol) was added to a solution of C72 (12.6 g, 24.2 mmol), 1,3-dimethylpyrimidine-2,4,6(1H,3H,5H)-trione (7.62 g, 48.8 mmol), and triphenylphosphine (1.92 g, 7.32 mmol) in dichloromethane (100 mL). The reaction mixture was heated to 35 C. for 5 hours, whereupon it was concentrated in vacuo and purified via silica gel chromatography (Gradient: 0% to 100% ethyl acetate in petroleum, followed by a gradient of 0% to 10% methanol in dichloromethane) to afford the product as an orange solid. Yield: 9.40 g, 21.7 mmol, 90%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.24 (br d, J=8.5 Hz, 2H), 6.87 (br d, J=8.5 Hz, 2H), 5.53-5.41 (m, 1H), 4.50 (AB quartet, J.sub.AB=11.7 Hz, .sub.AB=26.7 Hz, 2H), 4.02-3.94 (m, 1H), 3.87-3.62 (m, 4H), 3.80 (s, 3H), 3.42-3.17 (m, 4H), 2.18-2.05 (m, 1H), 1.86-1.59 (m, 4H), 1.55-1.46 (m, 1H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-(pyridin-2-ylamino)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C74)

(245) A mixture of C73 (100 mg, 0.231 mmol), 2-chloropyridine (52.5 mg, 0.462 mmol), [1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene](3-chloropyridyl)palladium(II) dichloride (15.8 mg, 23.2 mol), and cesium carbonate (226 mg, 0.694 mmol) in toluene (9 mL) was heated at 130 C. for 18 hours. The reaction mixture was filtered, concentrated in vacuo, and subjected to preparative thin layer chromatography (Eluent: ethyl acetate), followed by a second preparative thin layer chromatographic purification [Eluent: (1:1 ethyl acetate/petroleum ether) containing 0.5% ammonium hydroxide] to provide the product as a light yellow gum. Yield: 36 mg, 71 mol, 31%. LCMS m/z 532.2 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.10 (d, J=4 Hz, 1H), 7.43 (dd, J=8, 8 Hz, 1H), 7.24 (d, J=8.4 Hz, 2H), 6.88 (br d, J=8 Hz, 2H), 6.64-6.59 (m, 1H), 6.38 (d, J=8 Hz, 1H), 5.53-5.43 (m, 1H), 4.64-4.58 (m, 1H), 4.55 (d, half of AB quartet, J=12 Hz, 1H), 4.51-4.40 (m, 2H), 4.19-4.12 (m, 1H), 3.81 (s, 3H), 3.8-3.65 (m, 4H), 3.44-3.31 (m, 2H), 2.27-2.15 (m, 1H), 1.85-1.51 (m, 5H, assumed; partially obscured by water peak).

Step 5. Synthesis of (2R)-1, 1,1-trifluoro-3-hydroxypropan-2-yl 3-(pyridin-2-ylamino)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (27)

(246) Trifluoroacetic acid (1 mL) was added to a 0 C. solution of C74 (18 mg, 35 mol) in dichloromethane (2 mL). The reaction mixture was stirred for 45 minutes, whereupon it was treated with aqueous sodium bicarbonate solution (10 mL) and extracted with dichloromethane (315 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo; purification via reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: 0.225% formic acid in water; Mobile phase B: acetonitrile; Gradient: 8% to 28% B) afforded the product as a white solid. Yield: 10.0 mg, 25.7 mol, 73%. LCMS m/z 389.9 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD), characteristic peaks: 7.93 (br d, J=5 Hz, 1H), 7.42 (br dd, J=8, 7 Hz, 1H), 6.59-6.52 (m, 2H), 5.33-5.24 (m, 1H), 4.49-4.40 (m, 1H), 4.14 (dd, J=9, 6 Hz, 1H), 3.91-3.83 (m, 1H), 3.81-3.67 (m, 4H), 2.26 (dd, J=13, 8 Hz, 1H).

Example 28

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-(4-fluorobenzyl)-1-oxa-3-thia-4,9-diazaspiro[5.5]undecane-9-carboxylate 3,3-dioxide (28)

(247) ##STR00068##

Step 1. Synthesis of tert-butyl 4-({[(chloromethyl)sulfonyl]amino}methyl)-4-hydroxypiperidine-1-carboxylate (C75)

(248) Pyridine (3.0 mL, 37 mmol) was added to a solution of tert-butyl 4-(aminomethyl)-4-hydroxypiperidine-1-carboxylate (2 g, 8.7 mmol) in dichloromethane (40 mL), and the reaction mixture was cooled to 0 C. A solution of chloromethanesulfonyl chloride (0.930 mL, 10.2 mmol) in dichloromethane (40 mL) was then added drop-wise over 25 minutes, and the reaction mixture was allowed to stir at 0 C. for 5 minutes before being warmed to room temperature and stirred for 2 days. After solvents had been removed in vacuo, the residue was partitioned between dichloromethane and saturated aqueous ammonium chloride solution. The aqueous layer was extracted with dichloromethane, and the combined organic layers were dried over sodium sulfate, filtered, and concentrated under reduced pressure. Silica gel chromatography (Eluents: 50%, then 75%, then 90% ethyl acetate in heptane) provided the product as a tacky yellow solid. Yield: 851 mg, 2.48 mmol, 28%. LCMS m/z 341.5 [MH+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.28 (br t, J=6.2 Hz, 1H), 4.58 (s, 2H), 3.83 (br ddd, J=13.6, 4, 4 Hz, 2H), 3.24-3.15 (m, 4H), 1.69-1.61 (m, 2H), 1.56 (ddd, J=13.5, 11.1, 4.7 Hz, 2H), 1.46 (s, 9H).

Step 2. Synthesis of tert-butyl 1-oxa-3-thia-4,9-diazaspiro[5.5]undecane-9-carboxylate 3,3-dioxide (C76)

(249) A solution of C75 (360 mg, 1.05 mmol) in tetrahydrofuran (7 mL) was cooled to 0 C. and treated with sodium hydride (60% suspension in mineral oil; 109 mg, 2.72 mmol). After the reaction mixture had been stirred for two days at room temperature, more sodium hydride (60% suspension in mineral oil; 109 mg, 2.72 mmol) was added, and stirring was continued for 2 days at room temperature. Saturated aqueous ammonium chloride solution was added, and the mixture was diluted with ethyl acetate; the aqueous layer was extracted with ethyl acetate, and the combined organic layers were washed with saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered, and concentrated in vacuo. Chromatography on silica gel (Gradient: 25% to 50% ethyl acetate in heptane) afforded the product as a white solid. Yield: 430 mg, assumed quantitative. GCMS m/z 306.1 [M+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 4.67 (s, 2H), 4.63 (br t, J=7 Hz, 1H), 3.99-3.81 (m, 2H), 3.45 (br d, J=7 Hz, 2H), 3.06 (br dd, J=12, 11 Hz, 2H), 2.08-1.92 (m, 2H), 1.49 (ddd, J=14.0, 11.8, 4.7 Hz, 2H), 1.47 (s, 9H).

Step 3. Synthesis of tert-butyl 4-(4-fluorobenzyl)-1-oxa-3-thia-4,9-diazaspiro[5.5]undecane-9-carboxylate 3,3-dioxide (C77)

(250) A mixture of C76 (100 mg, 0.326 mmol), sodium iodide (74 mg, 0.49 mmol), cesium carbonate (319 mg, 0.979 mmol), and acetonitrile (3 mL) was treated with 1-(bromomethyl)-4-fluorobenzene (63 L, 0.51 mmol) and stirred at room temperature overnight. The reaction mixture was then filtered through diatomaceous earth, and the filter pad was rinsed with acetonitrile. The combined filtrates were concentrated in vacuo, and the residue was purified twice via silica gel chromatography (#1-Gradient: 10% to 33% ethyl acetate in heptane; #2-dichloromethane as eluent, followed by a gradient of 5% to 33% ethyl acetate in heptane) to afford the product as a white solid. Yield: 128 mg, 0.309 mmol, 95%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.32-7.27 (m, 2H), 7.07 (br dd, J=8.6, 8.6 Hz, 2H), 4.68 (s, 2H), 4.27-4.17 (br s, 2H), 3.74-3.59 (m, 2H), 3.14-2.99 (m, 4H), 2.04-1.88 (m, 2H), 1.43 (s, 9H), 1.33 (ddd, J=14.1, 11.3, 4.6 Hz, 2H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-(4-fluorobenzyl)-1-oxa-3-thia-4,9-diazaspiro[5.5]undecane-9-carboxylate 3,3-dioxide (C78)

(251) Conversion of C77 to C78 was effected using the method described for synthesis of C34 from C33 in Examples 8 and 9. .sup.1H NMR (400 MHz, CD.sub.3OD) of intermediate 4-(4-fluorobenzyl)-1-oxa-3-thia-4,9-diazaspiro[5.5]undecane 3,3-dioxide, trifluoroacetic acid salt, 7.44-7.38 (m, 2H), 7.11 (br dd, J=8.8, 8.8 Hz, 2H), 4.82 (s, 2H), 4.26 (br s, 2H), 3.24-3.17 (m, 2H), 3.23 (s, 2H), 3.17-3.08 (m, 2H), 2.34-2.26 (m, 2H), 1.58 (ddd, J=15, 13, 5 Hz, 2H); LCMS m/z 315.3 [M+H].sup.+. In this case, purification was effected via chromatography on silica gel (Eluents: 10%, then 25%, then 50% ethyl acetate in heptane), affording C78 as a tacky white solid. Yield: 156 mg, 0.264 mmol, 85%. LCMS m/z 613.1 [M+Na.sup.+] .sup.1H NMR (400 MHz, CDCl.sub.3) 7.29 (br dd, J=8.6, 5.3 Hz, 2H), 7.26-7.16 (br m, 2H), 7.07 (br dd, J=8.6, 8.6, 2H), 6.91-6.81 (br m, 2H), 5.49-5.38 (m, 1H), 4.73-4.63 (m, 2H), 4.55-4.39 (m, 2H), 4.33-4.14 (m, 2H), 3.88-3.7 (m, 2H), 3.81 (s, 3H), 3.73 (dd, half of ABX pattern, J=11.1, 3.8 Hz, 1H), 3.65 (dd, half of ABX pattern, J=11.1, 7.2 Hz, 1H), 3.22-2.99 (m, 4H), 2.12-1.91 (m, 2H), 1.40-1.23 (m, 2H).

Step 5. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-(4-fluorobenzyl)-1-oxa-3-thia-4,9-diazaspiro[5.5]undecane-9-carboxylate 3,3-dioxide (28)

(252) Trifluoroacetic acid (1 mL) was added portion-wise to a 0 C. solution of C78 (151 mg, 0.256 mmol) in dichloromethane (4 mL). The reaction mixture was stirred for 1 hour at room temperature, whereupon it was concentrated in vacuo, and the residue was partitioned between saturated aqueous sodium bicarbonate solution and ethyl acetate. The organic layer was dried over sodium sulfate, filtered, concentrated under reduced pressure, and chromatographed on silica gel (Eluents: 10%, then 25%, then 50% ethyl acetate in heptane) to afford the product as a tacky white solid. Yield: 109 mg, 0.232 mmol, 91%. LCMS m/z 471.4 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.30 (br dd, J=8.5, 5.4 Hz, 2H), 7.08 (br dd, J=8.6, 8.5 Hz, 2H), 5.27-5.17 (m, 1H), 4.74-4.63 (m, 2H), 4.34-4.13 (m, 2H), 3.98 (dd, half of ABX pattern, J=12.5, 3.3 Hz, 1H), 3.92-3.73 (m, 3H), 3.27-3.01 (m, 4H), 2.15-1.96 (m, 2H), 1.43-1.3 (m, 2H).

Example 29

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 4-[(4-fluorophenyl)sulfonyl]-3-hydroxy-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (29)

(253) ##STR00069##

(254) MicroCyp Reaction Buffer mix (Codexis; 519.0 mg) was mixed with deionized water (28.1 mL) to provide a buffer solution containing NADP.sup.+, glucose, glucose dehydrogenase, and potassium phosphate. Compound 6 (6.0 mg, 13 mol) was dissolved in a mixture of dimethyl sulfoxide (0.72 mL) and the buffer solution (0.24 mL).

(255) MCYP-P1.2-B12 (Codexis; 6.8 mg, 0.72 nmol/mg) was treated with the buffer solution prepared above (27.4 mL), followed by the solution of 6 prepared above. The reaction mixture was divided in half (14.2 mL each) and transferred into two 25 mL glass vials; the reaction mixtures were left open to the atmosphere and shaken on an orbital shaker (30 C., 225 rpm) for 24 hours. The combined reaction mixtures contained:

(256) [MCYP-P1.2-B12]=0.24 mg/mL (0.17 M, 6.8 mg, 4.89 nmol)

(257) [6]=0.21 mg/mL (0.44 mM, 6.0 mg, 13 mol)

(258) 2.5% dimethyl sulfoxide

(259) [NADP.sup.+]=0.75 mg/mL (0.99 mM, 21.5 mg, 28.1 mol)

(260) [Glucose]=3.55 mg/mL (19.7 mM, 100.8 mg, 559.7 mol)

(261) [Glucose dehydrogenase]=0.39 mg/mL (11.2 mg)

(262) 0.1 M potassium phosphate buffer, pH 8.0

(263) After 24 hours, the crude reaction mixtures were combined and purified via reversed phase HPLC (Column: Phenomenex Luna (2) C18, 5 m; Mobile phase A: 0.1% formic acid in water; Mobile phase B: 0.1% formic acid in acetonitrile; Gradient: 50% to 100% B) to afford the product as a solid (3.0 mg), presumed to be a mixture of diastereomers. 1-Dimensional and 2-dimensional NMR spectroscopic studies established the regiochemistry of oxidation as shown for 29. The .sup.1H NMR indicated that some impurities were present; peaks belonging to the product were identified via 2D NMR. Yield, corrected by quantitative NMR: 1.6 mg, 3.3 mol, 25%. LCMS m/z 469.2 [(MH.sub.2O)+H].sup.+ and 509.1 [M+Na.sup.+]. .sup.1H NMR (500 MHz, DMSO-d.sub.6), characteristic peaks: 7.93-7.88 (m, 2H), 7.42 (br dd, J=8.9, 8.8 Hz, 2H), 5.25-5.17 (m, 1H), 5.17 (br s, 1H), 3.83-3.78 (m, 1H), 3.70-3.53 (m, 5H), 3.26-3.13 (m, 2H), 3.19 (d, J=12.0 Hz, 1H), 2.79 (d, J=12.0 Hz, 1H), 1.53-1.41 (m, 2H).

Example 30

(2R)-3,3,3-Trifluoro-2-[({(3R)-3-[methyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]dec-8-yl}carbonyl)oxy]propyl phosphate, disodium salt (30)

(264) ##STR00070##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-(phosphonooxy)propan-2-yl (3R)-3-[methyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C79)

(265) Diphosphoryl tetrachloride (98%, 850 L, 6.02 mmol) was added to a 0 C. solution of 15 (560 mg, 1.20 mmol) in acetonitrile (7.5 mL), and the reaction mixture was stirred at 0 C. for 3 hours, whereupon it was poured into ice. After it had been stirred at room temperature for 1.75 hours, the resulting mixture was concentrated in vacuo to remove acetonitrile. The aqueous residue was extracted 4 times with ethyl acetate, and the combined organic layers were dried over magnesium sulfate, filtered and concentrated under reduced pressure. The resulting clear oil was treated with diethyl ether and again concentrated in vacuo; this diethyl ether treatment was repeated, affording the product as a white solid. Yield: 510 mg, 0.933 mmol, 78%. LCMS m/z 547.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CD.sub.3OD) 7.85-7.80 (m, 2H), 7.70-7.65 (m, 1H), 7.63-7.57 (m, 2H), 5.53-5.43 (m, 1H), 4.75-4.64 (m, 1H), 4.30-4.16 (m, 2H), 3.80 (dd, J=10.0, 7.4 Hz, 1H), 3.77-3.63 (m, 2H), 3.55 (dd, J=10.1, 5.0 Hz, 1H), 3.38-3.18 (m, 2H, assumed; partially obscured by solvent peak), 2.76 (s, 3H), 1.91 (br dd, J=13.3, 9.3 Hz, 1H), 1.78-1.57 (m, 3H), 1.51 (dd, J=13.5, 6.8 Hz, 1H), 1.48-1.37 (m, 1H).

Step 2. Synthesis of (2R)-3, 3, 3-trifluoro-2-[({(3R)-3-[methyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]dec-8-yl}carbonyl)oxy]propyl phosphate, disodium salt (30)

(266) To a solution of C79 (820 mg, 1.50 mmol) in ethanol (9 mL) was added aqueous sodium hydroxide solution (1 M; 2.9 mL, 2.9 mmol) and the reaction mixture was stirred at room temperature for 3 hours. Ethanol (10 mL) was added, and the mixture was concentrated in vacuo; this ethanol treatment was repeated three times; the resulting solid was washed with ethanol and collected via filtration, affording the product as a white solid. Yield: 660 mg, 1.12 mmol, 75%. LCMS m/z 547.2 [M+H].sup.+. .sup.1H NMR (400 MHz, D.sub.2O) 7.85-7.80 (m, 2H), 7.75-7.69 (m, 1H), 7.65-7.60 (m, 2H), 5.46-5.36 (m, 1H), 4.78-4.65 (m, 1H, assumed; partially obscured by solvent peak), 4.15-4.08 (m, 1H), 4.07-3.99 (m, 1H), 3.85 (dd, J=10, 8 Hz, 1H), 3.63-3.25 (m, 5H), 2.76 (s, 3H), 1.94 (dd, J=13.6, 9.3 Hz, 1H), 1.79-1.57 (m, 3H), 1.57-1.40 (m, 1H), 1.49 (dd, J=13.7, 6.7 Hz, 1H).

Example 31

(2R)-3,3,3-Trifluoro-2-[({(3R)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]dec-8-yl}carbonyl)oxy]propyl phosphate, disodium salt (31)

(267) ##STR00071##

Step 1. Synthesis of dibenzyl (2R)-3,3,3-trifluoro-2-hydroxypropyl phosphate (C80)

(268) (2R)-2-(Trifluoromethyl)oxirane (14.85 g, 132.5 mmol) was added to dibenzyl hydrogen phosphate (99%, 10.8 g, 38.4 mmol) in an amber bottle, and the thick slurry was heated in a 65 C. oil bath for 25 hours. Excess (2R)-2-(trifluoromethyl)oxirane was removed via concentration in vacuo. The resulting oil was diluted with dichloromethane (10 mL) and subjected to silica gel chromatography (Eluents: 5%, then 10%, then 15%, then 20% ethyl acetate in dichloromethane) to afford a pale yellow oil, which was treated with heptane (90 mL) and vigorously stirred. The resulting solids were allowed to granulate for 1.5 hours, whereupon they were collected via filtration and washed with heptane (38 mL), affording the product as a white solid. Yield: 9.11 g, 23.3 mmol, 61%. Melting point: 45 C. by differential scanning calorimetry. .sup.1H NMR (400 MHz, CD.sub.3CN) 7.42-7.34 (m, 10H), 5.06 (d, J=8.3 Hz, 4H), 4.27-4.14 (m, 2H), 4.14-4.05 (m, 1H).

Step 2. Synthesis of N-[(3R)-1-oxa-8-azaspiro[4.5]dec-3-yl]benzenesulfonamide, trifluoroacetic acid salt (C81)

(269) Conversion of C48 (1.3 g, 3.3 mmol) to C81 was carried out using the method described for synthesis of C10 from C9 in Example 1. The product was obtained as a colorless oil, and taken on without additional purification. The .sup.1H NMR indicated that the product was impure. Yield: 2.17 g, assumed quantitative. LCMS m/z 297.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3), product peaks only: 7.89-7.85 (m, 2H), 7.68-7.62 (m, 1H), 7.60-7.54 (m, 2H), 3.98-3.91 (m, 1H), 3.88 (dd, half of ABX pattern, J=9.7, 5.6 Hz, 1H), 3.62 (br dd, J=9.8, 4.4 Hz, 1H), 3.38-3.24 (m, 4H), 2.05 (dd, J=13.6, 7.3 Hz, 1H), 1.99-1.88 (m, 2H), 1.88-1.81 (m, 1H), 1.81-1.71 (m, 2H).

Step 3. Synthesis of (2R)-3-{[bis(benzyloxy)phosphoryl]oxy}-1,1,1-trifluoropropan-2-yl (3R)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C82)

(270) Compound C80 (1.90 g, 4.87 mmol) was added to a solution of 1,1-carbonyldiimidazole (790 mg, 4.87 mmol) in acetonitrile (23 mL). The reaction mixture was allowed to stir for 1.5 hours at room temperature, whereupon a solution of C81 (from the previous step, 2.00 g) in acetonitrile (2 mL) was added in a drop-wise manner over 1 minute. After the reaction mixture had been stirred for an additional 5 hours at room temperature, it was partitioned between ethyl acetate (250 mL) and water (250 mL). The organic layer was dried over sodium sulfate, filtered, and concentrated in vacuo; silica gel chromatography (Gradient: 30% to 80% ethyl acetate in heptane) provided the product as a colorless oil. Yield: 2.02 g. 2.83 mmol, 66% over 2 steps. LCMS m/z 713.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.88 (br d, J=8 Hz, 2H), 7.64-7.58 (m, 1H), 7.57-7.51 (m, 2H), 7.40-7.30 (m, 10H), 5.46-5.36 (m, 1H), 5.09-4.96 (m, 4H), 4.73-4.62 (m, 1H), 4.28-4.16 (m, 2H), 3.99-3.86 (m, 1H), 3.85-3.60 (m, 3H), 3.56-3.45 (m, 1H), 3.31-3.14 (m, 2H), 1.99-1.83 (m, 1H), 1.67-1.45 (m, 4H), 1.44-1.3 (m, 1H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-(phosphonooxy)propan-2-yl (3R)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C83)

(271) A solution of C82 (1.80 g, 2.53 mmol) in methanol (50 mL) was treated with 10% palladium on carbon (180 mg) and hydrogenated at 25 psi using a Parr reactor for 4 hours at room temperature. The reaction mixture was filtered through diatomaceous earth, and the filtrate was concentrated in vacuo to provide an oil, which was taken up in methanol (20 mL) and again concentrated under reduced pressure. The product was obtained as a brittle foam. Yield: 1.14 g, 2.14 mmol, 85%. LCMS m/z 533.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.87 (br d, J=8 Hz, 2H), 7.63-7.57 (m, 1H), 7.56-7.49 (m, 2H), 5.53-5.41 (m, 1H), 4.39-4.15 (m, 2H), 3.98-3.18 (m, 7H), 2.06-1.92 (m, 1H), 1.88-1.43 (m, 5H).

Step 5. Synthesis of (2R)-3,3,3-trifluoro-2-[({(3R)-3-[(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]dec-8-yl}carbonyl)oxy]propyl phosphate, disodium salt (31)

(272) Sodium tert-butoxide (2 M solution in tetrahydrofuran, 1.98 mL, 3.96 mmol) was added drop-wise over 5 minutes to a 0 C. solution of C83 (1.08 g, 2.03 mmol) in acetonitrile (20 mL), and the reaction mixture was allowed to warm to room temperature and stir for 2 hours. The resulting solid was collected on a Teflon filter, affording the product as a white solid. Yield: 1.02 g, 1.77 mmol, 87%. LCMS m/z 532.9 [M+H].sup.+. .sup.1H NMR (400 MHz, D.sub.2O) 7.90 (br d, J=8 Hz, 2H), 7.77-7.71 (m, 1H), 7.66 (br dd, J=8, 8 Hz, 2H), 5.47-5.37 (m, 1H), 4.14-4.05 (m, 1H), 4.02-3.86 (m, 3H), 3.68-3.31 (m, 5H), 2.08-1.97 (m, 1H), 1.80-1.49 (m, 5H).

Example 32

(2R)-3,3, 3-Trifluoro-2-[({4-[(4-fluorophenyl) sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undec-9-yl}carbonyl)oxy]propyl phosphate, disodium salt (32)

(273) ##STR00072##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-(phosphonooxy)propan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C84)

(274) 4-Methylmorpholine (14.5 mL, 132 mmol) was added to a solution of 6 (12.3 g, 26.1 mmol) in acetonitrile (750 mL) and the reaction mixture was cooled to 10 C. in an ice-salt bath. Phosphorus oxychloride (2.9 mL, 31 mmol) was added over 1 minute with vigorous stirring, and the reaction mixture was allowed to stir at 10 C. for one hour, whereupon it was poured into ice water (500 mL) and stirred for 1.5 hours to ensure complete quench of excess reagent. After concentration of the mixture to approximately one-half its original volume, the remaining liquid was extracted with ethyl acetate (1 L), and the organic layer was washed sequentially with aqueous hydrochloric acid (1 M; 3300 mL) and with saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered, and concentrated in vacuo to afford the product as a brittle foam (15.0 g) containing some ethyl acetate by .sup.1H NMR analysis. Yield, corrected for ethyl acetate: 14.2 g, 25.8 mmol, 99%. LCMS m/z 550.9 [M+H].sup.+. .sup.1H NMR (500 MHz, CDCl.sub.3), characteristic peaks: 7.81-7.73 (m, 2H), 5.57-5.48 (m, 1H), 4.45-4.34 (m, 1H), 4.32-4.20 (m, 1H), 3.97-3.74 (m, 4H), 3.35-3.11 (m, 2H), 3.06-2.89 (m, 2H), 2.89-2.72 (m, 2H), 2.03-1.87 (m, 2H), 1.68-1.46 (m, 2H).

Step 2. Synthesis of (2R)-3, 3, 3-trifluoro-2-[({4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undec-9-yl}carbonyl)oxy]propyl phosphate, disodium salt (32)

(275) A stirring solution of C84 (20.0 g, 36.3 mmol) in water (1.2 L) was treated with solid sodium bicarbonate until the pH of the mixture was approximately 7. The mixture was washed with ethyl acetate (500 mL), and the aqueous layer was acidified to pH 1.5-2 via portion-wise addition of concentrated hydrochloric acid. It was then extracted with ethyl acetate (1.5 L); the organic layer was washed with saturated aqueous sodium chloride solution (100 mL), dried over sodium sulfate, filtered, and concentrated in vacuo to provide a white solid (20 g). This material was dissolved in acetonitrile (600 mL), cooled to 0 C., and treated in a drop-wise manner over 5 minutes with a solution of sodium tert-butoxide in tetrahydrofuran (2 M; 35.4 mL, 70.9 mmol). After the reaction had stirred for one hour at 0 C., it was concentrated under reduced pressure to afford a solid (21.4 g). This material was mixed with ethanol (30 mL) and stirred at room temperature for 30 minutes, whereupon the solid was collected via filtration to provide the product as a solid (21.3 g) that contained some solvents via .sup.1H NMR analysis. Yield, corrected for solvents: 20.8 g, 35.0 mmol, 96%. LCMS m/z 551.3 [M+H].sup.+. .sup.1H NMR (500 MHz, D.sub.2O) 7.87-7.81 (m, 2H), 7.37 (dd, J=8.9, 8.7 Hz, 2H), 5.46-5.39 (m, 1H), 4.12-4.05 (m, 1H), 4.01-3.94 (m, 1H), 3.93-3.8 (m, 1H), 3.83 (dd, J=5.0, 4.8 Hz, 2H), 3.78-3.65 (m, 1H), 3.34-3.13 (m, 2H), 3.10-2.98 (m, 2H), 2.97-2.85 (m, 2H), 1.99-1.81 (m, 2H), 1.75-1.51 (m, 2H).

Alternate Synthesis of Example 32

(2R)-3, 3, 3-Trifluoro-2-[({4-[(4-fluorophenyl) sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undec-9-yl}carbonyl)oxy]propyl phosphate, disodium salt (32)

(276) ##STR00073##

Step 1. Synthesis of 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane, para-toluenesulfonic acid salt (C85)

(277) Potassium carbonate (24.0 g, 174 mmol) was added to a solution of tert-butyl 4-(aminomethyl)-4-hydroxypiperidine-1-carboxylate (5.00 g, 21.7 mmol) in acetonitrile (35 mL), and the reaction mixture was allowed to stir for 5 minutes. A solution of 4-fluorobenzenesulfonyl chloride (4.31 g, 22.1 mmol) in acetonitrile (15 mL) was slowly added over five minutes, and the resulting suspension was stirred at 25 C.; after 1 hour, 1,2-dibromoethane (7.50 mL, 87.0 mmol) was added, and the reaction mixture was heated at 80 C. for 27 hours, whereupon it was cooled to 25 C. and filtered. The reaction flask was rinsed with acetonitrile (218 mL), and the combined filtrates were concentrated under reduced pressure and diluted with ethyl acetate (72 mL). para-Toluenesulfonic acid monohydrate (8.38 g, 44.0 mmol) was added in one portion, and the reaction mixture was stirred at room temperature for 10 minutes, until a solution was obtained. It was then heated at 50 C. for 1.5 hours, at which point it was cooled to 25 C. and stirred for 2 hours to granulate the precipitate. This material was collected via filtration and rinsed with ethyl acetate, affording the product as a white solid. Yield: 7.26 g, 14.9 mmol, 69%. .sup.1H NMR (600 MHz, CD.sub.3OD) 7.84 (br dd, J=8, 5 Hz, 2H), 7.71 (br d, J=7.9 Hz, 2H), 7.38 (br dd, J=8.5, 8.5 Hz, 2H), 7.24 (br d, J=7.9 Hz, 2H), 3.81 (dd, J=5.0, 4.7 Hz, 2H), 3.26-3.20 (m, 2H), 3.19-3.12 (m, 2H), 3.03-2.98 (m, 2H), 2.86 (br s, 2H), 2.37 (s, 3H), 2.20 (br d, J=14.4 Hz, 2H), 1.74-1.67 (m, 2H).

Step 2. Synthesis of (2R)-3-{[bis(benzyloxy)phosphoryl]oxy}-1,1,1-trifluoropropan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C86)

(278) A solution of C80 (28.0 g, 71.7 mmol) in acetonitrile (75 mL) was added over 15 minutes to a mixture of 1,1-carbonyldiimidazole (97%, 12.6 g, 77.7 mmol) in acetonitrile (93 mL). The C80 solution was rinsed in with acetonitrile (5 mL) and the reaction mixture was allowed to stir at room temperature for 30 minutes. Compound C85 (37.0 g, 76.0 mmol) was added in one portion, and stirring was continued at room temperature for 6 hours, whereupon the reaction mixture was concentrated in vacuo. The residue was mixed with ethyl acetate (520 mL), and the mixture was washed twice with water (2260 mL), then concentrated under reduced pressure. The residue was dissolved in a mixture of ethyl acetate and heptane (1:1, 206 mL) and eluted through a pad of silica gel (150 g) using a mixture of ethyl acetate and heptane (1:1, 1.3 L). Fractions containing the product were combined and concentrated under reduced pressure to provide the product. Yield: 42.1 g, 57.6 mmol, 80%. .sup.1H NMR (600 MHz, CD.sub.3CN) 7.80-7.74 (m, 2H), 7.44-7.34 (m, 10H), 7.34 (dd, J=8.8, 8.7 Hz, 2H), 5.52-5.46 (m, 1H), 5.09-4.99 (m, 4H), 4.35-4.21 (m, 2H), 3.77-3.67 (m, 4H), 3.16-3.02 (m, 2H), 2.96-2.86 (m, 2H), 2.79-2.63 (m, 2H), 1.86-1.72 (m, 2H), 1.51-1.26 (m, 2H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-(phosphonooxy)propan-2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C84)

(279) A solution of C86 (2.0 grams, 2.7 mmol) in tetrahydrofuran (26 mL) was added to 5% palladium on carbon (Evonik Noblyst P1142; 40 mg) in a Biotage Atlantis reactor. Additional tetrahydrofuran (4.0 mL) was used to rinse the vessel containing starting material; this was added to the reaction mixture. The reactor was purged three times with nitrogen while the reaction mixture was stirred, and then three times with hydrogen without stirring. The hydrogen pressure was brought to 5 psig at 25 C., and then to 15 psig. The agitation was increased to 1200 rpm for 4 hours, whereupon the reactor was purged three times with nitrogen, and the reaction mixture was filtered. The filter cake was rinsed with tetrahydrofuran (20 mL), the combined filtrates were concentrated in vacuo, and the residue was dissolved in tert-butyl methyl ether (300 mL) and concentrated again. This dissolution/concentration was repeated, affording the product as a white foam. Yield: 1.35 g, 2.45 mmol, 91%.

Step 4. Synthesis of (2R)-3, 3, 3-trifluoro-2-[({4-[(4-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undec-9-yl}carbonyl)oxy]propyl phosphate, disodium salt (32)

(280) Aqueous sodium hydroxide solution (1 M, 12.0 mL, 12.0 mmol) was added drop-wise over 1 minute to a solution of C84 (97%, 3.50 g, 6.17 mmol) in ethanol (35.0 mL). The reaction mixture was stirred at room temperature for 1.5 hours; ethanol (120 mL) was added, and stirring was continued for 30 minutes, whereupon the reaction mixture was filtered. The filter cake was washed with ethanol (25 mL) to provide the product as a white solid. Yield: 2.88 g, 4.84 mmol, 78%. .sup.1H NMR (600 MHz, D.sub.2O) 7.85 (br dd, J=7, 5 Hz, 2H), 7.38 (br dd, J=9, 8 Hz, 2H), 5.47-5.39 (m, 1H), 4.12-4.06 (m, 1H), 4.01-3.95 (m, 1H), 3.94-3.66 (m, 2H), 3.84 (br dd, J=5, 4 Hz, 2H), 3.35-3.15 (m, 2H), 3.11-3.00 (m, 2H), 2.98-2.86 (m, 2H), 2.00-1.82 (m, 2H), 1.76-1.52 (m, 2H).

Example 33

(2R)-3,3, 3-Trifluoro-2-[({4-[(4-fluorophenyl) sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undec-9-yl}carbonyl)oxy]propyl phosphate, (bis)-L-lysine salt (33)

(281) ##STR00074##

(282) A solution of L-lysine (3.63 g, 24.8 mmol) in water (14 mL) was added to a solution of C84 (7.00 g, 12.7 mmol) in methanol (56 mL). The lysine solution was rinsed in with water (3 mL), and the reaction mixture was stirred at room temperature. Methanol (280 mL) was added to improve stirring of the slurry, and stirring was continued at room temperature for 1 hour. The reaction mixture was heated to 40 C. and stirred for 30 minutes, then cooled to 0 C. to 5 C. with stirring. After being held at 0 C. for 30 minutes, it was warmed to room temperature and stirred for 30 minutes, whereupon it was filtered through a Bchner funnel. The collected material was washed with methanol (140 mL) to afford a white solid (9.44 g). The bulk of this material (8.44 g) was slurried in methanol (140 mL) and stirred at room temperature for 4 hours, whereupon it was filtered through a Bchner funnel, providing the product as a white solid. Yield: 8.24 g, 9.77 mmol, 86% (corrected for material that was removed prior to reslurry). LCMS m/z 551.2 [M+H].sup.+. .sup.1H NMR (400 MHz, D.sub.2O) 7.88-7.81 (m, 2H), 7.38 (br dd, J=8.8, 8.8 Hz, 2H), 5.48-5.38 (m, 1H), 4.13-4.05 (m, 1H), 4.03-3.94 (m, 1H), 3.94-3.8 (m, 1H), 3.84 (br dd, J=5.0, 4.9 Hz, 2H), 3.79-3.64 (m, 1H), 3.71 (dd, J=6.2, 6.0 Hz, 2H), 3.36-3.13 (m, 2H), 3.10-3.02 (m, 2H), 2.99 (dd, J=7.7, 7.5 Hz, 4H), 2.95-2.86 (m, 2H), 2.01-1.81 (m, 6H), 1.76-1.54 (m, 6H), 1.54-1.34 (m, 4H).

Example 34

(2R)-3,3, 3-Trifluoro-2-[({4-[(3-fluorophenyl) sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undec-9-yl}carbonyl)oxy]propyl phosphate, disodium salt (34)

(283) ##STR00075##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-(phosphonooxy)propan-2-yl 4-[(3-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (C87)

(284) Diphosphoryl tetrachloride (2.63 mL, 19.0 mmol) was added drop-wise over 5 minutes to a 0 C. solution of 11 (1.74 g, 3.70 mmol) in acetonitrile (20 mL), and the reaction mixture was stirred at 0 C. for 3 hours, whereupon it was poured into ice (20 g) and stirred at room temperature for 1.75 hours. The reaction mixture was concentrated in vacuo, and the aqueous residue was partitioned between ethyl acetate (50 mL) and aqueous hydrochloric acid (1 M; 10 mL); the organic layer was washed sequentially with aqueous hydrochloric acid (1 M; 10 mL) and saturated aqueous sodium chloride solution (210 mL), then dried over sodium sulfate, filtered, and concentrated in vacuo. The resulting thick oil was taken up in water (75 mL), basified via addition of saturated aqueous sodium bicarbonate solution and solid sodium bicarbonate, and washed with ethyl acetate (50 mL). The pH of the aqueous layer was then adjusted to 2 using concentrated hydrochloric acid, and the product was extracted with ethyl acetate (250 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. The residue was mixed with ethyl acetate and filtered through a 0.45 m membrane filter; the filtrate was concentrated under reduced pressure to provide the product as a white solid. Yield: 1.36 g, 2.47 mmol, 67%. LCMS m/z 551.1 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.76-7.70 (m, 1H), 7.65-7.55 (m, 3H), 5.50-5.40 (m, 1H), 4.13-3.98 (m, 2H), 3.77-3.63 (m, 4H), 3.21-3.02 (m, 2H), 2.98-2.86 (m, 2H), 2.84-2.73 (m, 2H), 1.88-1.71 (m, 2H), 1.62-1.38 (m, 2H).

Step 2. Synthesis of (2R)-3, 3, 3-trifluoro-2-[({4-[(3-fluorophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undec-9-yl}carbonyl)oxy]propyl phosphate, disodium salt (34)

(285) Aqueous sodium hydroxide solution (1 M, 4.78 mL, 4.78 mmol) was added drop-wise over 3 minutes to a solution of C87 (1.35 g, 2.45 mmol) in ethanol (15 mL), and the reaction mixture was allowed to stir at room temperature for 1 hour. Ethanol (50 mL) was then added to the suspension, which was allowed to stir for 5 minutes before being filtered. The filter cake was rinsed with ethanol (10 mL) to afford the product as a white solid. Yield: 1.01 g, 1.70 mmol, 69%. LCMS m/z 551.1 [M+H].sup.+. .sup.1H NMR (400 MHz, D.sub.2O) 7.67 (ddd, half of ABXY pattern, J=8.0, 7.8, 5.2 Hz, 1H), 7.62 (ddd, half of ABXY pattern, J=7.8, 1.4, 1.3 Hz, 1H), 7.60-7.56 (m, 1H), 7.49 (dddd, J=8.7, 8.0, 2.5, 1 Hz, 1H), 5.47-5.38 (m, 1H), 4.13-4.05 (m, 1H), 4.02-3.93 (m, 1H), 3.93-3.64 (m, 2H), 3.84 (dd, J=5.2, 4.8 Hz, 2H), 3.36-3.13 (m, 2H), 3.13-3.01 (m, 2H), 3.01-2.87 (m, 2H), 2.02-1.81 (m, 2H), 1.77-1.50 (m, 2H).

Method A

(286) Method A describes a specific synthetic method for preparations of certain exemplar compounds of the invention.

Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 4-(R.SUP.70.-sulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate analogues (MA-2) via sulfonylation of C31 followed by deprotection

(287) ##STR00076##

(288) A solution of the sulfonic acid MA-1 (0.15 mmol) in N,N-dimethylformamide (0.15 mL) was treated with thionyl chloride (0.12 ml, 1.6 mmol), and the reaction mixture was heated with shaking at 50 C. for 16 hours. Volatiles were removed using a Genevac evaporator; 1,2-dichloroethane (2 mL) was added, and the mixture was concentrated again. A solution of C31 (25.9 mg, 60.0 mmol) in 1,2-dichloroethane (0.5 mL) was added to the crude sulfonyl chloride, followed by N,N-diisopropylethylamine (0.225 mL, 1.29 mmol), and the reaction mixture was shaken overnight at room temperature. It was then partitioned between half-saturated aqueous sodium bicarbonate solution (1.5 mL) and ethyl acetate (2.4 mL) and subjected to vortexing. The organic layer was eluted through a solid phase extraction cartridge (6 mL) charged with sodium sulfate (1 g); this extraction procedure was repeated twice, and the combined eluents were concentrated in vacuo. A mixture of trifluoroacetic acid and 1,2-dichloroethane (1:1, 1 mL) was added, and the reaction mixture was shaken at room temperature for 2 hours, whereupon it was concentrated in vacuo and subjected to purification via reversed phase HPLC (Column: Waters Sunfire C18, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 5% to 100% B).

(289) TABLE-US-00006 TABLE 6 Method of synthesis, structure, and physicochemical properties for Examples 35-91. Method of Synthesis; Non- .sup.1H NMR (400 MHz, CDCl.sub.3) ; Mass commercial spectrum, observed ion m/z [M + H].sup.+ or Example starting HPLC retention time; Mass spectrum m/z Number materials Structure [M + H].sup.+ (unless otherwise indicated) 35 Footnotes.sup.1,2 8.25 (d, J = 5.1 Hz, 1H), 6.41 (d, J = 5.0 Hz, 1H), 5.33-5.22 (m, 1H), 4.34-4.19 (m, 2H), 4.06-3.99 (m, 1H), 3.89 (br dd, half of ABX pattern, J = 12, 7 Hz, 1H), 3.83-3.74 (m, 8H), 3.08-2.88 (m, 2H), 2.68 (tt, J = 11.5, 3.6 Hz, 1H), 1.99-1.88 (m, 2H), 1.84-1.69 (m, 2H); 405.1 36 C19.sup.3,4 By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. .sup.1H NMR (400 MHz, CD.sub.3OD) 8.03 (d, J = 2.5 Hz, 1H), 7.72-7.66 (m, 2H), 7.20 (br dd, J = 8.9, 8.5 Hz, 2H), [6.27 (d, J = 2.5 Hz) and 6.26 (d, J = 2.5 Hz), total 1H], 5.50-5.40 (m, 1H), 4.43-4.33 (m, 1H), 3.83-3.76 (m, 2H), 3.63-3.55 (m, 2H), 2.08-2.01 (m, 2H), [1.77 (dd, J = 3.6, 3.5 Hz) and 1.72 (dd, J = 3.4, 3.4 Hz), total 1H]; 468.0 37 Example 3.sup.5; C6, C17 By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. .sup.1H NMR (400 MHz, CD.sub.3OD) 8.51 (br d, J = 5 Hz, 1H), 7.79 (ddd, J = 7.8, 7.8, 1.5 Hz, 1H), 7.64 (d, J = 2.3 Hz, 1H), 7.33 (ddd, J = 7.3, 5.0, 0.6 Hz, 1H), 7.03-6.98 (m, 1H), [6.11 (d, J = 2.3 Hz) and 6.10 (d, J = 2.4 Hz), total 1H], 5.37 (s, 2H), 5.32-5.22 (m, 1H), 3.90-3.71 (m, 4H), 3.65- 3.52 (m, 2H), 1.98-1.91 (m, 2H), [1.76 (dd, J = 3.5, 3.4 Hz) and 1.70 (dd, J = 3.6, 3.4 Hz), total 1H]; 396.9 38 Example 6.sup.6,7; C13 0 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.58 (d, J = 2.4 Hz, 1H), 6.13 (d, J = 2.4 Hz, 1H), 5.35-5.26 (m, 1H), 4.38-4.28 (m, 1H), 4.27-4.14 (m, 2H), 4.09-4.01 (m, 2H), 3.88 (br dd, half of ABX pattern, J = 12, 4 Hz, 1H), 3.79 (dd, half of ABX pattern, J = 12.4, 6.9 Hz, 1H), 3.60- 3.51 (m, 2H), 3.13-2.94 (m, 2H), 2.92-2.83 (m, 1H), 2.10-1.87 (m, 6H), 1.76-1.53 (m, 2H); 391.9 39 Examples 19, 20 and 21; C19, C59 By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. .sup.1H NMR (600 MHz, DMSO-d.sub.6) 8.33-8.30 (m, 1H), 7.81-7.76 (m, 2H), 7.30 (br dd, J = 8.9, 8.8 Hz, 2H), [6.33 (d, J = 2.4 Hz) and 6.31 (d, J = 2.4 Hz), total 1H], 5.88-5.83 (m, 1H), 5.82-5.73 (m, 1H), 3.83-3.56 (m, 6H), 2.06-1.98 (m, 2H), [1.82 (dd, J = 3.4, 3.4 Hz) and 1.76 (dd, J = 3.4, 3.3 Hz), total 1H]; 450.2 40 Example 13; C17 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.94-7.87 (m, 2H), 7.40-7.32 (m, 2H), 5.29-5.18 (m, 1H), 3.90-3.81 (m, 1H), 3.79-3.69 (m, 1H), 3.68- 3.51 (m, 2H), 3.49-3.25 (m, 3H, assumed; partially obscured by solvent peak), 3.21- 3.00 (m, 3H), 2.96-2.82 (m, 2H); 426.9 41 Example 6.sup.8; C2 7.32-7.21 (m, 2H, assumed; partially obscured by solvent peak), 7.05-6.95 (m, 2H), 5.27-5.16 (m, 1H), 4.03-3.93 (m, 1H), 3.90-3.80 (m, 1H), 3.55-3.35 (m, 2H), 3.40 (br s, 2H), 3.35-3.16 (m, 2H), 2.51-2.32 (m, 3H), 2.21-2.06 (m, 2H), 1.71-1.41 (m, 6H, assumed; partially obscured by water peak), 1.41-1.30 (m, 2H); 419.2 42 Example 23; C23, C2 7.31-7.23 (m, 2H, assumed; partially obscured by solvent peak), 7.01 (br dd, J = 8.8, 8.5 Hz, 2H), 5.28-5.18 (m, 1H), 3.99 (br dd, half of ABX pattern, J = 12.3 Hz, 1H), 3.91-3.77 (m, 3H), 3.77-3.70 (m, 2H), 3.46- 3.36 (m, 2H), 3.33-3.15 (m, 2H), 2.48-2.38 (m, 2H), 2.25-2.15 (m, 2H), 2.13-1.97 (m, 2H), 1.48-1.36 (m, 2H); 421.1 43 Example 13.sup.9; C23, C2 7.31-7.23 (m, 2H, assumed; partially obscured by solvent peak), 7.01 (br dd, J = 8.8, 8.5 Hz, 2H), 5.27-5.18 (m, 1H), 4.02- 3.95 (m, 1H), 3.90-3.69 (m, 5H), 3.36-3.13 (m, 3H), 2.69-2.55 (m, 1H), 2.40-2.05 (m, 5H), 1.98-1.86 (m, 1H), 1.45-1.3 (m, 2H), 1.31 (d, J = 6.5 Hz, 3H); 435.2 44 Example 13.sup.10 7.26 (br dd, J = 8.5, 5.6 Hz, 2H), 7.00 (br dd, J = 8.8, 8.7 Hz, 2H), 5.28-5.17 (m, 1H), 4.03- 3.94 (m, 1H), 3.90-3.67 (m, 5H), 3.33-3.14 (m, 3H), 2.65-2.44 (m, 2H), 2.36-2.27 (m, 1H), 2.24-2.05 (m, 3H), 2.02-1.84 (m, 1H), 1.44-1.32 (m, 2H), 1.29 (d, J = 6.5 Hz, 3H); 435.2 45 Example 13.sup.10 7.30-7.22 (m, 2H), 7.00 (br dd, J = 8.7, 8.7 Hz, 2H), 5.27-5.17 (m, 1H), 4.02-3.94 (m, 1H), 3.89-3.67 (m, 5H), 3.32-3.12 (m, 3H), 2.68-2.53 (m, 1H), 2.36-2.25 (m, 1H), 2.24- 2.05 (m, 3H), 1.98-1.63 (m, 2H), 1.44-1.32 (m, 2H), 1.28 (d, J = 6.6 Hz, 3H); 435.2 46 Example 24.sup.11; C2 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.42-7.33 (m, 2H), 7.02 (br dd, J = 8.5, 8.5 Hz, 2H), 5.33- 5.22 (m, 1H), 4.01-3.91 (m, 2H), 3.91-3.72 (m, 6H), 3.3-3.12 (m, 4H, assumed; partially obscured by solvent peak), 2.83-2.71 (m, 1H), 2.55 (s, 2H), 1.78-1.69 (m, 2H), 1.67- 1.43 (m, 6H); 479.1 47 Example 13; C2 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.95-7.88 (m, 2H), 7.32 (br dd, J = 8.8, 8.8 Hz, 2H), 5.33- 5.24 (m, 1H), 4.21-4.08 (m, 2H), 3.88 (br dd, half of ABX pattern, J = 12, 4 Hz, 1H), 3.78 (dd, half of ABX pattern, J = 12.3, 6.7 Hz, 1H), 2.95-2.76 (m, 2H), 2.77 (d, J = 6.5 Hz, 2H), 1.79-1.61 (m, 3H), 1.24-1.01 (m, 2H); 429.0 48 Example 14; C2 0 By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. [8.01 (d, J = 6.0 Hz) and 7.96 (d, J = 6.0 Hz), total 1H], 6.35-6.23 (m, 2H), 5.32-5.21 (m, 1H), 4.03-3.64 (m, 7H), 3.61-3.38 (m, 3H), 3.18-3.07 (m, 2H), 1.93-1.82 (m, 1H), 1.22-1.11 (m, 2H), 0.92- 0.83 (m, 2H); 386.0 49 Example 13; C23, C2 5.32-5.20 (m, 1H), 4.14-4.04 (m, 2H), 4.04- 3.95 (m, 1H), 3.95-3.80 (m, 3H), 3.76 (br dd, J = 4.9, 4.8 Hz, 2H), 3.45-3.32 (m, 4H), 3.32- 3.10 (m, 5H), 2.53-2.34 (m, 1H), 2.05-1.80 (m, 6H), 1.60-1.43 (m, 2H); 461.1 50 Example 23.sup.12; C2 7.31-7.23 (m, 2H, assumed; partially obscured by solvent peak), 7.00 (br dd, J = 8.5, 8.5 Hz, 2H), 5.27-5.18 (m, 1H), 4.18- 3.95 (m, 5H), 3.90-3.81 (m, 1H), 3.60 (s, 2H), 3.35-3.26 (m, 2H), 2.87-2.59 (m, 3H), 2.47-2.38 (m, 1H), 2.35 (d, J = 6.8 Hz, 2H), 1.84-1.73 (m, 2H), 1.70-1.46 (m, 4H, assumed; partially obscured by solvent peak), 1.09-0.92 (m, 2H); 463.2 51 Example 13; C23, C2 7.63-7.53 (m, 2H), 7.18 (dd, J = 9.0, 8.7 Hz, 1H), 5.31-5.21 (m, 1H), 4.06-3.96 (m, 1H), 3.95-3.82 (m, 3H), 3.80 (br dd, J = 5.0, 5.0 Hz, 2H), 3.32-3.13 (m, 2H), 3.09-2.91 (m, 2H), 2.89-2.71 (m, 2H), 2.43-2.24 (m, 1H), 2.37 (br s, 3H), 2.05-1.91 (m, 2H), 1.69-1.43 (m, 2H, assumed; partially obscured by water peak); 485.1 52 Example 13; C23, C2 7.59 (ddd, J = 9.2, 7.2, 2.1 Hz, 1H), 7.56-7.50 (m, 1H), 7.38 (ddd, J = 9.0, 8.8, 7.4 Hz, 1H), 5.32-5.21 (m, 1H), 4.05-3.97 (m, 1H), 3.96- 3.83 (m, 3H), 3.81 (dd, J = 4.9, 4.9 Hz, 2H), 3.32-3.13 (m, 2H), 3.10-2.95 (m, 2H), 2.89- 2.75 (m, 2H), 2.5-2.2 (br m, 1H), 2.05-1.92 (m, 2H), 1.6-1.43 (m, 2H); 489.1 53 Example 13; C2 8.42 (br s, 1H), 7.79 (br d, J = 8.8 Hz, 1H), 6.81 (d, J = 8.8 Hz, 1H), 5.41-5.32 (m, 1H), 5.32-5.23 (m, 1H), 4.06-3.98 (m, 1H), 3.93- 3.72 (m, 3H), 3.59-3.40 (m, 2H), 2.55-2.32 (m, 1H), 2.12-1.95 (m, 2H), 1.92-1.75 (m, 2H); 403.0 54 Method A 1.65 minutes.sup.13; 488.2 55 Method A 1.65 minutes.sup.13; 482.1 56 Method A 1.63 minutes.sup.13; 485.1 57 Example 10; C2 7.97-7.92 (m, 1H), 7.44-7.39 (m, 1H), 6.65 (d, J = 8.0 Hz, 1H), 5.32-5.20 (m, 2H), 4.02 (dd, half of ABX pattern, J = 12.4, 3.0 Hz, 1H), 3.89 (dd, half of ABX pattern, J = 12.5, 6.8 Hz, 1H), 3.88-3.72 (m, 2H), 3.57-3.41 (m, 2H), 2.25 (s, 3H), 2.07-1.96 (m, 2H), 1.94-1.73 (m, 2H, assumed; partially obscured by water peak); 348.9 58 Example 10; C2 00 8.46 (s, 1H), 7.57 (d, J = 2.0 Hz, 1H), 6.55 (d, J = 2.0 Hz, 1H), 5.32-5.22 (m, 2H), 4.07 (s, 3H), 4.05-3.98 (m, 1H), 3.93-3.73 (m, 3H), 3.63-3.45 (m, 2H), 2.32 (s, 3H), 2.10-1.87 (m, 4H); 430.1 59 Example 13; C23, C2 01 7.72 (d, J = 1.5 Hz, 1H), 7.52 (dd, half of ABX pattern, J = 8.0, 1.6 Hz, 1H), 7.43 (d, half of AB quartet, J = 8.3 Hz, 1H), 5.31-5.21 (m, 1H), 4.06-3.96 (m, 1H), 3.95-3.82 (m, 3H), 3.80 (dd, J = 5.0, 4.8 Hz, 2H), 3.32-3.13 (m, 2H), 3.10-2.94 (m, 2H), 2.89-2.73 (m, 2H), 2.48 (s, 3H), 2.39-2.26 (m, 1H), 2.05-1.92 (m, 2H), 1.6-1.44 (m, 2H, assumed; partially obscured by water peak); 523.1 [M + Na.sup.+] 60 Example 13; C23, C2 02 7.82 (dd, J = 6.6, 2.3 Hz, 1H), 7.65 (ddd, J = 8.6, 4.3, 2.3 Hz, 1H), 7.34 (dd, J = 8.5, 8.4 Hz, 1H), 5.31-5.21 (m, 1H), 4.05-3.97 (m, 1H), 3.96-3.83 (m, 3H), 3.81 (dd, J = 5.0, 4.9 Hz, 2H), 3.31-3.13 (m, 2H), 3.11-2.96 (m, 2H), 2.90-2.75 (m, 2H), 2.35-2.23 (m, 1H), 2.04-1.93 (m, 2H), 1.6-1.45 (m, 2H, assumed; partially obscured by water peak); 527.1 [M + Na.sup.+] 61 Example 10; C2 03 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.73 (br s, 1H), 7.34 (br s, 1H), 5.36-5.27 (m, 1H), 5.27-5.19 (m, 1H), 3.89 (dd, half of ABX pattern, J = 12.4, 3.8 Hz, 1H), 3.84-3.69 (m, 2H), 3.79 (dd, half of ABX pattern, J = 12.4, 6.6 Hz, 1H), 3.60-3.44 (m, 2H), 2.20 (s, 3H), 2.16 (s, 3H), 2.08-1.92 (m, 2H), 1.86-1.68 (m, 2H), 363.0 62 Example 13.sup.14; C2 04 7.84-7.77 (m, 2H), 7.23 (dd, J = 8.7, 8.5 Hz, 2H), 5.31-5.20 (m, 1H), 4.28-4.12 (m, 2H), 4.05-3.97 (m, 1H), 3.88 (dd, half of ABX pattern, J = 12.5, 7 Hz, 1H), 2.96-2.78 (m, 4H), 2.76 (s, 3H), 1.88-1.75 (m, 3H), 1.30- 1.15 (m, 2H); 443.1 63 Example 10; C2 05 8.33-8.30 (m, 1H), 7.87 (d, J = 2.3 Hz, 1H), 5.50-5.41 (m, 1H), 5.33-5.24 (m, 1H), 4.07- 3.98 (m, 1H), 3.94-3.85 (m, 1H), 3.83-3.54 (m, 4H), 2.38-2.27 (m, 1H), 2.09-1.87 (m, 3H); 437.0 64 Example 10.sup.15; C2 06 8.21 (d, J = 5.4 Hz, 1H), 7.54 (d, J = 1.9 Hz, 1H), 6.95 (dd, J = 5.3, 1.5 Hz, 1H), 6.80-6.78 (m, 1H), 6.41 (d, J = 2.0 Hz, 1H), 5.39-5.31 (m, 1H), 5.31-5.24 (m, 1H), 4.03 (dd, half of ABX pattern, J = 12, 3 Hz, 1H), 3.97 (s, 3H), 3.92-3.76 (m, 2H), 3.90 (dd, J = 13, 7 Hz, 1H), 3.59-3.42 (m, 2H), 2.12-1.98 (m, 2H), 1.92-1.79 (m, 2H); 415.1 65 Example 10.sup.16; C2 07 7.65 (dd, J = 7.9, 7.8 Hz, 1H), 7.49 (d, J = 1.6 Hz, 1H), 7.20 (d, J = 7.4 Hz, 1H), 6.70 (d, J = 8.3 Hz, 1H), 6.57 (d, J = 1.8 Hz, 1H), 5.37- 5.24 (m, 2H), 4.21 (s, 3H), 4.06-3.97 (m, 1H), 3.93-3.69 (m, 3H), 3.62-3.45 (m, 2H), 2.72-2.64 (m, 1H), 2.09-1.96 (m, 2H), 1.95- 1.83 (m, 2H); 415.1 66 C31.sup.17 08 3.22 minutes.sup.18; 495 67 Example 7.sup.19; C31 09 7.42-7.30 (m, 5H), 5.30-5.20 (m, 1H), 4.58- 4.49 (m, 1H), 4.24 (d, J = 5.9 Hz, 2H), 4.04- 3.95 (m, 1H), 3.92-3.77 (m, 3H), 3.71 (dd, J = 4.9, 4.8 Hz, 2H), 3.31-3.09 (m, 4H), 2.95 (br s, 2H), 2.44-2.30 (m, 1H), 1.98-1.87 (m, 2H), 1.53-1.36 (m, 2H); 481.9 68 Example 7.sup.20; C31 0 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.73 (br AB quartet, J.sub.AB = 8.5 Hz, v.sub.AB = 22.4 Hz, 4H), 5.33-5.24 (m, 1H), 3.92-3.73 (m, 7H), 3.3- 3.14 (m, 2H), 3.05-2.93 (m, 2H), 2.89-2.76 (m, 2H), 2.02-1.88 (m, 2H), 1.64-1.45 (m, 2H); 477.5 69 Example 3; C6, C17 By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. 8.39 (d, J = 2.7 Hz, 1H), 7.90-7.85 (m, 1H), 7.71 (d, J = 2.3 Hz, 1H), 6.83 (d, J = 8.9 Hz, 1H), [6.20 (d, J = 2.3 Hz) and 6.19 (d, J = 2.3 Hz), total 1H], 5.31- 5.21 (m, 1H), 4.04-3.98 (m, 1H), 3.97 (s, 3H), 3.92-3.81 (m, 3H), 3.66-3.58 (m, 2H), 2.07-2.00 (m, 2H), 1.90-1.84 (m, 1H); 413.0 70 Example 18.sup.21,22; C2 By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. 7.84-7.76 (m, 2H), 7.20 (br dd, J = 8.5, 8.5 Hz, 2H), 5.31-5.21 (m, 1H), 4.04-3.63 (m, 7H), 3.54-3.45 (m, 1H), 3.31-2.96 (m, 3H), 2.59-2.47 (m, 1H), 1.62-1.23 (m, 4H), 1.04-0.91 (m, 3H); 485.0 71 Example 18.sup.21,22; C2 By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. 7.84-7.76 (m, 2H), 7.21 (br dd, J = 8.8, 8.3 Hz, 2H), 5.31-5.19 (m, 1H), 4.04-3.94 (m, 1H), 3.94-3.63 (m, 6H), 3.55-3.45 (m, 1H), 3.32-2.99 (m, 3H), 2.60-2.43 (m, 2H), 1.62-1.50 (m, 1H), 1.48- 1.34 (m, 2H), 1.03-0.91 (m, 3H); 485.0 72 Example 13.sup.23,24; C2 .sup.1H NMR (600 MHz, DMSO-d.sub.6) 7.32 (br dd, J = 8.4, 5.7 Hz, 2H), 7.19 (br dd, J = 9.0, 8.8 Hz, 2H), 5.26-5.17 (m, 2H), 4.35 (s, 2H), 3.77-3.71 (m, 1H), 3.71-3.61 (m, 3H), 3.38- 3.24 (m, 2H, assumed; obscured by water peak), 3.24-3.20 (m, 2H), 1.86-1.63 (m, 4H); 421.2 73 C31.sup.25 3.20 minutes.sup.26; 485 74 Example 7.sup.27; C31 8.59-8.54 (m, 1H), 7.75-7.69 (m, 1H), 7.30- 7.23 (m, 2H, assumed; partially obscured by solvent peak), 5.75 (br t, J = 5 Hz, 1H), 5.29- 5.20 (m, 1H), 4.37 (d, J = 5.0 Hz, 2H), 4.04- 3.96 (m, 1H), 3.91-3.77 (m, 3H), 3.74-3.69 (m, 2H), 3.30-3.11 (m, 4H), 3.04-2.98 (m, 2H), 1.98-1.89 (m, 2H), 1.52-1.33 (m, 2H); 483.0 75 Example 26; C2 1.72 minutes.sup.13; 365.1 76 Example 27; C31 8.15 (s, 2H), 5.31-5.19 (m, 1H), 4.04-3.94 (m, 1H), 3.91-3.72 (m, 7H), 3.72-3.62 (m, 2H), 3.40-3.22 (m, 2H), 2.56-2.37 (br s, 1H), 2.13 (s, 3H), 1.96-1.83 (m, 2H), 1.63-1.48 (m, 2H); 405.0 77 C31.sup.17 3.26 minutes.sup.26; 481 78 C31.sup.17 0 3.30 minutes.sup.26; 511 79 Example 68; C31 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.83 (br s, 1H), 7.80-7.75 (m, 2H), 7.62 (dd, J = 7.9, 7.7 Hz, 1H), 5.33-5.24 (m, 1H), 3.92-3.76 (m, 6H), 3.75 (s, 1H), 3.29-3.14 (m, 2H), 3.05- 2.94 (m, 2H), 2.88-2.77 (m, 2H), 2.01-1.89 (m, 2H), 1.65-1.46 (m, 2H); 477.5 80 Example 13.sup.28; C2 .sup.1H NMR (400 MHz, CD.sub.3OD) 8.14 (br s, 1H), 7.63 (AB quartet, J.sub.AB = 7.7 Hz, v.sub.AB = 66.8 Hz, 4H), 6.40 (br s, 1H), 5.36- 5.27 (m, 1H), 4.28-4.15 (m, 2H), 3.89 (dd, half of ABX pattern, J = 12.4, 3.7 Hz, 1H), 3.79 (dd, half of ABX pattern, J = 12.3, 6.9 Hz, 1H), 3.53 (s, 1H), 3.18-2.93 (m, 3H), 2.08-1.96 (m, 2H), 1.85-1.61 (m, 2H); 408.2 81 Example 3; C6, C17 7.84-7.80 (m, 1H), 7.58 (br AB quartet, J.sub.AB = 8.3 Hz, v.sub.AB = 21.3 Hz, 4H), 6.24-6.19 (m, 1H), 5.32-5.21 (m, 1H), 4.05-3.97 (m, 1H), 3.93-3.81 (m, 3H), 3.68-3.58 (m, 2H), 3.12 (s, 1H), 2.09-2.02 (m, 2H), 1.90-1.84 (m, 1H); 406.0 82 Example 3.sup.29; C6 7.82 (d, J = 2.3 Hz, 1H), 7.58 (br AB quartet, J.sub.AB = 8.6 Hz, v.sub.AB = 21.2 Hz, 4H), 6.24-6.19 (m, 1H), 5.32-5.21 (m, 1H), 4.05-3.97 (m, 1H), 3.93-3.81 (m, 3H), 3.69-3.57 (m, 2H), 3.12 (s, 1H), 2.09-2.02 (m, 2H), 1.90-1.84 (m, 1H); 406.0 83 Example 6; C23, C2 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.81-7.77 (m, 1H), 7.74-7.69 (m, 2H), 7.63 (dd, component of ABC system, J = 7.8, 7.8 Hz, 1H), 5.33-5.25 (m, 1H), 3.92-3.74 (m, 6H), 3.3-3.12 (m, 2H, assumed; partially obscured by solvent peak), 3.04-2.97 (m, 2H), 2.88-2.81 (m, 2H), 2.00-1.90 (m, 2H), 1.64-1.46 (m, 2H); 486.9 84 C31.sup.25 3.00 minutes.sup.26; 471 85 6.sup.30,31 Presumed to be a mixture of diastereomers around the phosphorus atom; .sup.1H NMR (400 MHz, CD.sub.3OD) 7.88-7.81 (m, 2H), 7.37 (br d, J = 9.0, 8.6 Hz, 2H), 5.53-5.43 (m, 1H), 4.20-4.12 (m, 1H), 4.12-4.03 (m, 1H), 3.94- 3.76 (m, 2H), 3.79 (dd, J = 5.1, 4.9 Hz, 2H), 3.62-3.52 (m, 3H), 3.29-3.12 (m, 2H), 3.06- 2.90 (m, 2H), 2.90-2.73 (m, 2H), 2.02-1.83 (m, 2H), 1.67-1.44 (m, 2H); 565.3 86 6.sup.30,31 .sup.1H NMR (400 MHz, CD.sub.3OD), characteristic peaks: 7.87-7.81 (m, 2H), 7.37 (dd, J = 8.7, 8.7 Hz, 2H), 5.59-5.50 (m, 1H), 4.48-4.31 (m, 2H), 3.93-3.73 (m, 8H), 3.3-3.15 (m, 2H), 3.04-2.92 (m, 2H), 2.87-2.76 (m, 2H), 2.01-1.91 (m, 2H), 1.64-1.47 (m, 2H); 579.3 87 6.sup.30,32 Presumed to be a mixture of diastereomers around the phosphorus atom; .sup.1H NMR (400 MHz, CD.sub.3OD) 7.87-7.81 (m, 2H), 7.37 (dd, J = 8.8, 8.7 Hz, 2H), 5.52-5.43 (m, 1H), 4.20- 4.13 (m, 1H), 4.11-4.03 (m, 1H), 3.97-3.82 (m, 4H), 3.79 (dd, J = 5.1, 4.8 Hz, 2H), 3.3- 3.12 (m, 2H), 3.06-2.89 (m, 2H), 2.89-2.74 (m, 2H), 2.02-1.84 (m, 2H), 1.66-1.44 (m, 2H), 1.31-1.20 (m, 3H); 579.2 88 6.sup.30,32 0 .sup.1H NMR (400 MHz, CD.sub.3OD) 7.87-7.80 (m, 2H), 7.37 (dd, J = 8.7, 8.6 Hz, 2H), 5.58-5.49 (m, 1H), 4.44-4.28 (m, 2H), 4.21-4.07 (m, 4H), 3.93-3.8 (m, 2H), 3.80 (dd, J = 5.1, 4.8 Hz, 2H), 3.3-3.15 (m, 2H), 3.03-2.93 (m, 2H), 2.87-2.75 (m, 2H), 2.01-1.91 (m, 2H), 1.63-1.47 (m, 2H), 1.40-1.27 (m, 6H); 607.2 89 Example 85.sup.33; 6 Presumed to be a mixture of diastereomers around the phosphorus atom; .sup.1H NMR (400 MHz, CD.sub.3OD) 7.87-7.81 (m, 2H), 7.37 (dd, J = 8.8, 8.7 Hz, 2H), 5.56-5.46 (m, 1H), 4.28- 4.20 (m, 1H), 4.20-4.07 (m, 3H), 3.93-3.82 (m, 2H), 3.80 (dd, J = 5.0, 4.9 Hz, 2H), 3.43- 3.34 (m, 2H), 3.3-3.14 (m, 2H), 3.00-2.90 (m, 8H), 2.85-2.79 (m, 2H), 2.01-1.89 (m, 2H), 1.68-1.44 (m, 2H); 622.5 90 Example 85.sup.34; 6 Presumed to be a mixture of diastereomers around the phosphorus atom; .sup.1H NMR (400 MHz, CD.sub.3OD) 7.87-7.81 (m, 2H), 7.37 (dd, J = 8.7, 8.7 Hz, 2H), 5.55-5.46 (m, 1H), 4.33- 4.20 (m, 3H), 4.15-4.07 (m, 1H), 3.93-3.82 (m, 2H), 3.80 (dd, J = 5.2, 4.7 Hz, 2H), 3.69- 3.60 (m, 2H), 3.28-3.17 (m, 11H), 3.01-2.95 (m, 2H), 2.86-2.78 (m, 2H), 2.01-1.90 (m, 2H), 1.67-1.44 (m, 2H); 636.2 91 Example 30; 7 .sup.1H NMR (400 MHz, D.sub.2O) 7.82-7.77 (m, 2H), 7.77-7.72 (m, 1H), 7.68-7.62 (m, 2H), 5.47-5.37 (m, 1H), 4.12-4.04 (m, 1H), 4.01- 3.93 (m, 1H), 3.93-3.63 (m, 2H), 3.83 (dd, J = 5.1, 4.9 Hz, 2H), 3.36-3.12 (m, 2H), 3.11- 2.98 (m, 2H), 2.97-2.84 (m, 2H), 2.00-1.80 (m, 2H), 1.76-1.49 (m, 2H); 533.1 .sup.13,3,3-Trifluoropropane-1,2-diol was converted to 3-{[tert-butyl(dimethyl)silyl]oxy}-1,1,1-trifluoropropan-2-ol using the method described for synthesis of C59 from C58 in Examples 19, 20 and 21. .sup.2Reaction of 4-[4-(piperidin-4-yl)pyrimidin-2-yl]morpholine, hydrochloride salt with bis(trichloromethyl) carbonate and 3-{[tert-butyl(dimethyl)silyl]oxy}-1,1,1-trifluoropropan-2-ol (see footnote 1) in the presence of N,N-diisopropylethylamine afforded 3-{[tert-butyl(dimethyl)silyl]oxy}-1,1,1-trifluoropropan-2-yl 4-[2-(morpholin-4-yl)pyrimidin-4-yl]piperidine-1-carboxylate. This material was desilylated via treatment with acetic acid in a mixture of water and tetrahydrofuran to afford Example 35. .sup.3Examination of coupling constants in the NMR spectra of rel-(2S,3R)-1,1,1,4,4,4-hexafluorobutane-2,3-diol and rel-(2R,3R)-1,1,1,4,4,4-hexafluorobutane-2,3-diol allowed tentative assignment of the starting material used for Example 36 as the rel-(2S,3R) isomer. .sup.4Reaction of C19 with bis(trichloromethyl) carbonate and rel-(2S,3R)-1,1,1,4,4,4-hexafluorobutane-2,3-diol in the presence of N,N-diisopropylethylamine afforded Example 36. .sup.5Alkylation of C6 with 2-(chloromethyl)pyridine in the presence of potassium hydroxide in acetone provided the requisite tert-butyl (1,5,6)-6-[1-(pyridin-2-ylmethyl)-1H-pyrazol-3-yl]-3-azabicyclo[3.1.0]hexane-3-carboxylate. .sup.6Reaction of C13 with tetrahydro-2H-pyran-4-yl methanesulfonate in the presence of cesium carbonate and potassium iodide, at elevated temperature in N,N-dimethylformamide, provided the requisite tert-butyl 4-[1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-3-yl]piperidine-1-carboxylate. .sup.7The carbonate reagent employed in this case was 1-({[(3-{[tert-butyl(dimethyl)silyl]oxy}-1,1,1-trifluoropropan-2-yl)oxy]carbonyl}oxy)pyrrolidine-2,5-dione, prepared from 3-{[tert-butyl(dimethyl)silyl]oxy}-1,1,1-trifluoropropan-2-ol (see footnote 1) using the general method described for synthesis of C17 in Example 3. .sup.8tert-Butyl 2,9-diazaspiro[5.5]undecane-2-carboxylate was converted to 2-tert-butyl 9-{(2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl} 2,9-diazaspiro[5.5]undecane-2,9-dicarboxylate using the method described tor synthesis of C29 in Example 6. Treatment with trifluoroacetic acid removed both protecting groups, affording (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 2,9-diazaspiro[5.5]undecane-9-carboxylate, which was then subjected to reductive amination with 4-fluorobenzaldehyde and sodium triacetoxyborohydride to afford Example 41. .sup.9Reaction of C23 with 4-fluorobenzaldehyde in the presence of 1H-benzotriazole and acetic acid provided the corresponding imine; in situ treatment with methylmagnesium bromide then afforded the requisite tert-butyl 4-[1-(4-fluorophenyl)ethyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate. Example 43 is a diastereomeric mixture, with both stereochemistries present at the methyl group. .sup.10Separation of Example 43 into its component diastereomers was effected via supercritical fluid chromatography [Column: Chiral Technologies Chiralpak AD, 5 m; Mobile phase: 4:1 carbon dioxide:(0.1% ammonium hydroxide in ethanol)]. The first-eluting diastereomer was Example 44, and the second-eluting diastereomer was Example 45. .sup.11In this case, tert-butyl 1-oxa-6-azaspiro[2.5]octane-6-carboxylate was reacted with tetrahydro-2H-pyran-4-amine, and the resulting tert-butyl 4-hydroxy-4-[(tetrahydro-2H-pyran-4-ylamino)methyl]piperidine-1-carboxylate was N-alkylated with 1-(bromomethyl)-4-fluorobenzene in the presence of potassium carbonate; this afforded the requisite tert-butyl 4-{[(4-fluorobenzyl)(tetrahydro-2H-pyran-4-yl)amino]methyl}-4-hydroxypiperidine-1-carboxylate. .sup.12tert-Butyl 4-[(tetrahydro-2H-pyran-4-ylamino)methyl]piperidine-1-carboxylate was synthesized via reductive amination of tetrahydro-4H-pyran-4-one with tert-butyl 4-(aminomethyl)piperidine-1-carboxylate, using magnesium sulfate and silica gel followed by sodium triacetoxyborohydride. .sup.13Conditions for analytical HPLC. Column: Waters Atlantis dC18, 4.6 50 mm, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 5.0% to 95% B, linear over 4.0 minutes; Flow rate: 2 mL/minute. .sup.14Intermediate tert-butyl 4-({[(4-fluorophenyl)sulfonyl]amino}methyl)piperidine-1-carboxylate was reacted with iodomethane and potassium carbonate to provide tert-butyl 4-({[(4-fluorophenyl)sulfonyl](methyl)amino}methyl)piperidine-1-carboxylate. .sup.15The requisite 2-chloro-4-(1-methyl-1H-pyrazol-5-yl)pyridine was prepared via a Suzuki reaction between 2-chloro-4-iodopyridine and 1-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole, mediated via [1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II). .sup.16Suzuki reaction of 2-bromo-6-chloropyridine and 1-methyl-5-(4,4,5,5-tetramethy1-1,3,2-dioxaborolan-2-yl)-1H-pyrazole, mediated via [1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II), afforded 2-chloro-6-(1-methyl-1H-pyrazol-5-yl)pyridine. .sup.17The requisite sulfonic acid was prepared from the corresponding bromo compound via reaction with a mixture of potassium disulfite, palladium(II) acetate, triphenylphosphine, tetraethylammonium bromide, sodium formate and 1,10-phenanthroline in N,N-dimethylformamide at elevated temperature. The sulfonic acid was cooled to 0 C., treated with C31 and N-chlorosuccinimide, and stirred at 30 C. for 1 hour. The resulting sulfonamide was deprotected via treatment with trifluoroacetic acid in dichloromethane to provide the product of the Example. .sup.18Conditions for analytical HPLC. Column: Waters XBridge C18, 2.1 50 mm, 5 m; Mobile phase A: 0.0375% trifluoroacetic acid in water; Mobile phase B: 0.01875% trifluoroacetic acid in acetonitrile; Gradient: 10% to 100% B over 4.0 minutes; Flow rate: 0.8 mL/minute. .sup.19N-Benzyl-2-oxo-1,3-oxazolidine-3-sulfonamide was used as the sulfonylating reagent in this case, in a mixture of triethylamine and acetonitrile. .sup.20Reaction of C31 and 4-bromobenzenesulfonyl chloride provided (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-[(4-bromophenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate, which was then converted to (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-({4-[(trimethylsilyl)ethynyl]phenyl}sulfonyl)-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate by reaction with ethynyl(trimethyl)silane, copper(I) iodide, and [1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II). Desilylation was effected via treatment with potassium carbonate and methanol to afford (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 4-[(4-ethynylphenyl)sulfonyl]-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate; final deprotection using trifluoroacetic acid provided Example 68. .sup.21Reaction of tert-butyl 4-oxopiperidine-1-carboxylate with nitroethane and triethylamine provided tert-butyl 4-hydroxy-4-(1-nitroethyl)piperidine-1-carboxylate, which was hydrogenated to afford tert-butyl 4-(1-aminoethyl)-4-hydroxypiperidine-1-carboxylate. This material was treated with 4-fluorobenzenesulfonyl chloride and potassium carbonate, followed by 1,2-dibromoethane, to provide the requisite intermediate tert-butyl 4-[(4-fluorophenyl)sulfonyl]-5-methyl-1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate. .sup.22Separation to provide the diastereomers of Examples 70 and 71 was effected via supercritical fluid chromatography [Column: Chiral Technologies Chiralpak AD, 5 m; Mobile phase A: carbon dioxide; Mobile phase B: 2-propanol; Gradient: 25% to 100% B). The first-eluting diastereomer was Example 70, and the second-eluting diastereomer was Example 71. .sup.23tert-Butyl 2-oxo-1-oxa-3,8-diazaspiro[4.5]decane-8-carboxylate was treated with sodium hydride and 1-(chloromethyl)-4-fluorobenzene to afford tert-butyl 3-(4-fluorobenzyl)-2-oxo-1-oxa-3,8-diazaspiro[4.5]decane-8-carboxylate; deprotection with hydrogen chloride in 1,4-dioxane provided the requisite 3-(4-fluorobenzyl)-1-oxa-3,8-diazaspiro[4.5]decan-2-one. .sup.24In this case, the final deprotection was effected with hydrogen chloride in 1,4-dioxane, rather than trifluoroacetic acid. .sup.25Compound C31 was allowed to react with the appropriate sulfonyl chloride and triethylamine; the resulting sulfonamide was deprotected with trifluoroacetic acid in dichloromethane to afford the product of the Example. .sup.26Conditions for analytical HPLC. Column: Waters XBridge C18, 2.1 50 mm, 5 m; Mobile phase A: 0.0375% trifluoroacetic acid in water; Mobile phase B: 0.01875% trifluoroacetic acid in acetonitrile; Gradient: 1% to 5% B over 0.6 minutes; 5% to 100% B over 3.4 minutes; Flow rate: 0.8 mL/minute. .sup.272-Bromoethanol was slowly added to a solution of chlorosulfonyl isocyanate in dichloromethane at 0 C. After an hour, a mixture of 1-(pyridin-2-yl)methanamine and triethylamine was slowly added to the 0 C. reaction mixture. Removal of solvent provided 2-oxo-N-(pyridin-2-ylmethyl)-1,3-oxazolidine-3-sulfonamide, which was used as the sulfonylating reagent in this case, in a mixture of triethylamine and acetonitrile. .sup.28The requisite tert-butyl 4-[1-(4-ethynylphenyl)-1H-pyrazol-3-yl]piperidine-1-carboxylate was prepared as follows: tert-butyl 4-acetylpiperidine-1-carboxylate was converted to tert-butyl 4-[1-(4-bromophenyl)-1H-pyrazol-3-yl]piperidine-1-carboxylate using the methods described for synthesis of C18 from C4 that are found in Examples 1 and 3. Reaction with ethynyl(trimethyl)silane, copper(I) iodide and tetrakis(triphenylphosphine)palladium(0), followed by removal of the silyl group via treatment with potassium carbonate in methanol, provided tert-butyl 4-[1-(4-ethynylphenyl)-1H-pyrazol-3-yl]piperidine-1-carboxylate. .sup.29In this case, the enantiomer of C1 was employed; this may be prepared similarly, via the use of (2S)-2-(trifluoromethyl)oxirane rather than (2R)-2-(trifluoromethyl)oxirane. .sup.30A 0 C. solution of 6 in acetonitrile was treated with 5 equivalents of diphosphoryl tetrachloride; after one to two hours at 0 C., the appropriate alcohol (40 equivalents) was added, generating a mixture of the mono- and di-alkyl phosphate esters. .sup.31Reversed phase HPLC was used to separate the monomethyl and dimethyl phosphate esters. Column: Phenomenex Gemini NX C18, 5 m; Mobile phase A: 0.1% ammonium hydroxide in water; Mobile phase B: 0.1% ammonium hydroxide in acetonitrile; Gradient: 50% to 100% B. The first-eluting product was Example 85, and the second-eluting product was Example 86. .sup.32Reversed phase HPLC was used to separate the monoethyl and diethyl phosphate esters. Column: Phenomenex Gemini NX C18, 5 m; Mobile phase A: 0.1% ammonium hydroxide in water; Mobile phase B: 0.1% ammonium hydroxide in acetonitrile; Gradient: 50% to 100% B. The first-eluting product was Example 87, and the second-eluting product was Example 88. .sup.33In this case, 2-(dimethylamino)ethanol was used in place of methanol. The product was purified using reversed phase HPLC (Column: Phenomenex Luna C18, 5 m; Mobile phase A: 0.1% formic acid in water; Mobile phase B: 0.1% formic acid in acetonitrile; Gradient: 50% to 100% B). .sup.34In this case, 2-hydroxy-N,N,N-trimethylethanaminium chloride was used in place of methanol.

Example 92

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (3R)-3-[ethyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (92)

(290) ##STR00134##

Step 1. Synthesis of tert-butyl (3R)-3-[ethyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C88)

(291) Sodium hydride (60% dispersion in mineral oil; 15 mg, 0.38 mmol) was added to a 0 C. solution of C48 (50.0 mg, 0.126 mmol) in N,N-dimethylformamide (1 mL). After the reaction mixture had been stirred at 0 C. for 30 minutes, a solution of bromoethane (27.5 mg, 0.252 mmol) in N,N-dimethylformamide (0.1 mL) was added, and the reaction mixture was allowed to stir at 25 C. for 16 hours. It was subsequently cooled to 0 C., and additional sodium hydride (60% dispersion in mineral oil; 15 mg, 0.38 mmol) was added; stirring was continued at 0 C. for 30 minutes, whereupon a solution of bromoethane (20 mg, 0.18 mmol) in N,N-dimethylformamide (0.1 mL) was added. The reaction mixture was then stirred at 25 C. for 16 hours. Water (30 mL) was added, and the resulting mixture was extracted with dichloromethane (330 mL). The combined organic layers were dried over sodium sulfate, filtered, concentrated in vacuo, and subjected to preparative thin layer chromatography on silica gel (Eluent: 2:1 petroleum ether/ethyl acetate), providing the product as a light yellow gum. Yield: 40 mg, 94 mol, 75%. LCMS m/z 447.2 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.81 (br d, J=7 Hz, 2H), 7.58 (br dd, J=7.4, 7.3 Hz, 1H), 7.51 (br dd, J=7.9, 7.2 Hz, 2H), 4.64-4.54 (m, 1H), 3.80 (dd, J=9.8, 7.6 Hz, 1H), 3.66-3.5 (m, 2H), 3.50 (dd, J=9.9, 6.2 Hz, 1H), 3.28-3.10 (m, 4H), 1.94 (dd, J=13.2, 8.8 Hz, 1H), 1.63-1.53 (m, 3H), 1.47 (dd, J=13.3, 8.0 Hz, 1H), 1.43 (s, 9H), 1.42-1.33 (m, 1H), 1.30 (t, J=7.0 Hz, 3H).

Step 2. Synthesis of (2R)-1, 1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3R)-3-[ethyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C89)

(292) Trifluoroacetic acid (1 mL) was added in a drop-wise manner to a 0 C. solution of C88 (39.0 mg, 91.9 mol) in dichloromethane (1 mL), and the reaction mixture was stirred at 15 C. for 1 hour. Removal of volatiles under reduced pressure provided N-ethyl-N-[(3R)-1-oxa-8-azaspiro[4.5]dec-3-yl]benzenesulfonamide, trifluoroacetate salt, as a yellow gum, LCMS m/z 325.1 [M+H].sup.+. This material was dissolved in acetonitrile (1 mL), cooled to 0 C., and treated with C2 (reaction solution in acetonitrile containing 0.11 mmol) and triethylamine (73.3 mg, 0.724 mmol). After the reaction mixture had been stirred at 20 C. for 16 hours, it was concentrated in vacuo and purified via chromatography on silica gel (Gradient: 0% to 50% ethyl acetate in petroleum ether) to afford the product as a colorless gum. Yield: 35 mg, 58 mol, 63%. LCMS m/z 623.1 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.84-7.80 (m, 2H), 7.62-7.57 (m, 1H), 7.56-7.49 (m, 2H), 7.23 (br d, J=8.7 Hz, 2H), 6.87 (br d, J=8.7 Hz, 2H), 5.52-5.40 (m, 1H), 4.66-4.53 (m, 1H), 4.49 (AB quartet, upfield doublet is broadened, J.sub.AB=11.7 Hz, .sub.AB=28.4 Hz, 2H), 3.88-3.62 (m, 5H), 3.81 (s, 3H), 3.58-3.47 (m, 1H), 3.36-3.10 (m, 4H), 1.93 (dd, J=13.2, 8.8 Hz, 1H), 1.7-1.55 (m, 3H, assumed; partially obscured by water peak), 1.50 (dd, J=13.2, 8.1 Hz, 1H), 1.39 (ddd, J=13.5, 11.2, 4.3 Hz, 1H), 1.31 (t, J=7.1 Hz, 3H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3R)-3-[ethyl(phenylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (92)

(293) To a 0 C. suspension of C89 (35 mg, 58 mol) in dichloromethane (1 mL) was added trifluoroacetic acid (1 mL). The reaction mixture was stirred at 18 C. for 1 hour, whereupon it was cooled to 0 C. and slowly treated with aqueous sodium bicarbonate solution (30 mL), while the purple mixture became colorless. It was then extracted with dichloromethane (330 mL), and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. The residue was purified using reversed phase HPLC (Column: Agela Durashell, 5 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 44% to 84% B), to afford the product as a colorless oil. Yield: 7.5 mg, 16 mol, 28%. LCMS m/z 481.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.82 (br d, J=8 Hz, 2H), 7.60 (br dd, J=7.5, 7.5 Hz, 1H), 7.52 (br dd, J=7.5, 7.5 Hz, 2H), 5.30-5.17 (m, 1H), 4.66-4.55 (m, 1H), 4.04-3.93 (m, 1H), 3.91-3.69 (m, 4H), 3.58-3.48 (m, 1H), 3.39-3.09 (m, 4H), 2.50-2.36 (m, 1H), 2.01-1.88 (m, 1H), 1.7-1.6 (m, 2H, assumed; largely obscured by water peak), 1.57-1.35 (m, 2H), 1.31 (t, J=7.0 Hz, 3H).

Examples 93 and 94

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (3R)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (93) and (2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (3S)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (94)

(294) ##STR00135##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-[(cyclopropylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C90)

(295) Cyclopropanesulfonyl chloride (650 mg, 4.62 mmol) and triethylamine (1.17 g, 11.6 mmol) were added to an 18 C. suspension of C73 (1.00 g, 2.31 mmol) in dichloromethane (8 mL), and the reaction mixture was stirred at 10 C. for 12 hours. After the reaction mixture had been concentrated in vacuo, the residue was diluted with water (30 mL) and extracted with ethyl acetate (330 mL). The combined organic layers were dried over sodium sulfate, filtered, concentrated under reduced pressure, and purified using silica gel chromatography (Gradient: 0% to 15% methanol in dichloromethane), affording the product as a colorless gum. Yield: 641 mg, 1.19 mmol, 52%. LCMS m/z 559.1 [M+Na.sup.+].

Step 2. Synthesis of (2R)-1, 1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3R)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (DIAST-1) (C91) and (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3S)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (DIAST-2) (C92)

(296) To a 0 C. solution of C90 (641 mg, 1.19 mmol) in N,N-dimethylformamide (8 mL) was added sodium hydride (60% dispersion in mineral oil; 95.6 mg, 2.39 mmol), and the reaction mixture was stirred at 0 C. for 30 minutes. Iodomethane (254 mg, 1.79 mmol) was added at 0 C., and the reaction mixture was allowed to stir at 15 C. for 3 hours, whereupon it was diluted with water (50 mL) and extracted with ethyl acetate (330 mL). The combined organic layers were concentrated in vacuo, and the residue was purified by silica gel chromatography (Gradient: 0% to 50% ethyl acetate in petroleum ether) to afford a mixture of C91 and C92 as a colorless gum. Yield: 310 mg, 0.563 mmol, 47%. The component diastereomers were separated via supercritical fluid chromatography [Column: Chiral Technologies Chiralpak IC, 10 m; Mobile phase: 40% (0.1% ammonium hydroxide in 2-propanol) in carbon dioxide], affording C91 as the first-eluting diastereomer, and C92 as the second-eluting diastereomer, both as colorless gums. The indicated stereochemistries at the sulfonamide positions were assigned on the basis of a chiral synthesis of 93 (see Alternate Synthesis of Example 93 below).

(297) C91Yield: 147 mg, 0.267 mmol, 22%. LCMS m/z 573.0 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.25 (br d, J=8.5 Hz, 2H), 6.88 (br d, J=8.5 Hz, 2H), 5.55-5.42 (m, 1H), 4.73-4.62 (m, 1H), 4.51 (AB quartet, J.sub.AB=11.7 Hz, .sub.AB=29.3 Hz, 2H), 3.96 (dd, half of ABX pattern, J=10.0, 7.5 Hz, 1H), 3.85 (dd, half of ABX pattern, J=10.1, 5.2 Hz, 1H), 3.82 (s, 3H), 3.8-3.64 (m, 4H), 3.41-3.22 (m, 2H), 2.88 (s, 3H), 2.26 (tt, J=8.0, 4.9 Hz, 1H), 2.11-1.97 (m, 1H), 1.85-1.64 (m, 4H), 1.45 (ddd, J=13.7, 11.2, 4.4 Hz, 1H), 1.21-1.15 (m, 2H), 1.03-0.97 (m, 2H).
C92Yield: 155 mg, 0.282 mmol, 24%. LCMS m/z 573.0 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3), characteristic peaks: 7.25 (br d, J=8.7 Hz, 2H), 6.88 (br d, J=8.5 Hz, 2H), 5.54-5.43 (m, 1H), 4.73-4.63 (m, 1H), 4.51 (AB quartet, upfield d is broadened, J.sub.AB=11.9 Hz, .sub.AB=29.0 Hz, 2H), 4.01-3.91 (m, 1H), 3.89-3.78 (m, 2H), 3.82 (s, 3H), 3.79-3.64 (m, 3H), 3.40-3.20 (m, 2H), 2.88 (s, 3H), 2.26 (tt, J=8, 5 Hz, 1H), 2.14-1.95 (m, 1H), 1.84-1.7 (m, 4H, assumed; partially obscured by water peak), 1.21-1.15 (m, 2H), 1.03-0.97 (m, 2H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3R)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (93)

(298) Trifluoroacetic acid (2 mL) was added to a 0 C. solution of C91 (147 mg, 0.267 mmol) in dichloromethane (8 mL). The reaction mixture was stirred at 16 C. for 1 hour, whereupon it was cooled to 0 C., and slowly treated with aqueous sodium bicarbonate solution (20 mL), while the purple mixture became colorless. The resulting mixture was extracted sequentially with dichloromethane (20 mL) and ethyl acetate (220 mL); the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Chromatography on silica gel (Gradient: 0% to 70% ethyl acetate in petroleum ether) afforded the product as a yellow oil. Yield: 49.7 mg, 0.115 mmol, 43%. LCMS m/z 431.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.32-5.20 (m, 1H), 4.73-4.64 (m, 1H), 4.05-3.93 (m, 2H), 3.94-3.75 (m, 4H), 3.46-3.24 (m, 2H), 2.89 (s, 3H), 2.39-2.21 (m, 1H), 2.26 (tt, J=8.0, 4.9 Hz, 1H), 2.13-2.04 (m, 1H), 1.86-1.69 (m, 4H), 1.56-1.41 (m, 1H), 1.22-1.15 (m, 2H), 1.04-0.96 (m, 2H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3S)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (94)

(299) Conversion of C92 to the product was carried out using the method described for synthesis of 93 from C91. The product was isolated as a yellow oil. Yield: 63 mg, 0.15 mmol, 53%. LCMS m/z 431.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.31-5.20 (m, 1H), 4.74-4.64 (m, 1H), 4.05-3.93 (m, 2H), 3.92-3.71 (m, 4H), 3.42-3.20 (m, 2H), 2.89 (s, 3H), 2.37-2.22 (m, 2H), 2.08 (dd, J=13.3, 9.0 Hz, 1H), 1.86-1.67 (m, 3H), 1.81 (dd, J=13.6, 7.0 Hz, 1H), 1.55-1.45 (m, 1H, assumed; partially obscured by water peak), 1.22-1.15 (m, 2H), 1.04-0.96 (m, 2H).

Alternate Synthesis of Example 93

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (3R)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8GP-azaspiro[4.5]decane-8-carboxylate (93)

(300) ##STR00136## ##STR00137##

Step 1. Synthesis of tert-butyl (3R)-3-amino-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C93)

(301) A pH 8.0 buffer solution was prepared, containing 0.1 M aqueous potassium phosphate and 2 mM magnesium chloride. A stock solution of substrate was prepared as follows: tert-butyl 3-oxo-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (18.0 g, 70.5 mmol) was dissolved in water containing 4% dimethyl sulfoxide (14.4 mL). Warming and stirring were required for dissolution, and the resulting solution was maintained at 40 C. Propan-2-amine, hydrochloride salt (16.8 g, 176 mmol) was added to a mixture of pyridoxal 5-phosphate monohydrate (1.87 g, 7.05 mmol) and the pH 8.0 buffer (300 mL). The resulting pH was approximately 6.5; the pH was adjusted to 8 via addition of aqueous potassium hydroxide solution (6 M; approximately 4 mL). The stock solution of substrate was added via syringe, in 5 mL portions, resulting in a suspension, still at pH 8. Codex ATA-200 transaminase (batch #11099; 1.4 g) was almost completely dissolved in pH 8 buffer (20 mL), and poured into the reaction mixture. Additional pH 8 buffer (25.6 mL) was used to ensure complete transfer of the enzyme. The reaction mixture was stirred at 35 C. with a nitrogen sweep (32 mL/minute) through a needle placed approximately 0.5 cm above the reaction surface. Due to difficulties in stirring, vacuum (220 Torr, 300 mbar) was applied after 3 hours, to remove the acetone generated by the transamination reaction. The suspended solids were broken up manually, which improved the stirring of the reaction mixture. After 26 hours, the reaction mixture was allowed to cool to room temperature, and aqueous hydrochloric acid (6 M, 5 mL) was added, to bring the pH from 8 to 6.5. After addition of ethyl acetate (200 mL), the mixture was vigorously stirred for 5 minutes and then filtered through diatomaceous earth (43 g; this filter aid had been slurried in water prior to being introduced into the filter funnel. The water was then removed, providing a tightly packed bed). The filter pad was washed sequentially with water (120 mL) and ethyl acetate (100 mL), and the aqueous layer of the combined filtrates was adjusted to pH 9-9.5 with aqueous potassium hydroxide solution (6 M; approximately 10 mL). The aqueous layer was then treated with dichloromethane (200 mL), and the resulting mixture was vigorously stirred for 5 minutes before being filtered through a pad of diatomaceous earth. The filter pad was washed with dichloromethane (100 mL), and the aqueous layer of the combined filtrates was extracted twice with dichloromethane, in the same manner as that described above, with adjustment of the pH to 9-10 (this required approximately 2 mL of the 6 M aqueous potassium hydroxide solution in both cases). All of the dichloromethane extracts were combined and dried over sodium sulfate with vigorous stirring. Filtration and concentration in vacuo afforded the product as an oily yellow solid (14.76 g). A fourth extraction was carried out in the same manner, but in this case the aqueous layer was adjusted to a pH of >10. The product obtained from this extraction was a white solid (1.9 g). Combined yield: 16.61 g, 64.79 mmol, 92%. .sup.1H NMR (500 MHz, CDCl.sub.3) 3.95 (dd, J=9.0, 5.6 Hz, 1H), 3.69-3.63 (m, 1H), 3.62-3.52 (m, 3H), 3.38-3.27 (m, 2H), 2.6-2.2 (v br s, 2H), 2.07 (dd, J=13.0, 7.6 Hz, 1H), 1.78-1.71 (m, 1H), 1.69-1.56 (m, 2H), 1.55-1.47 (m, 2H), 1.45 (s, 9H).

Step 2. Synthesis of tert-butyl (3R)-3-amino-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, (2R)-5-oxopyrrolidine-2-carboxylate salt (C94)

(302) A solution of C93 (16.61 g, 64.79 mmol) in ethanol (400 mL) was heated to 63 C. and treated portion-wise with (2R)-5-oxopyrrolidine-2-carboxylic acid (7.78 g, 60.3 mmol). The reaction mixture was then removed from the heating bath, and allowed to cool overnight. The mixture was cooled to 12 C. in an ice bath, and filtered. The collected solids were washed with cold ethanol (250 mL) and then with diethyl ether (100 mL), affording the product as a pale yellow solid (19.2 g). The combined filtrates were concentrated in vacuo, with removal of approximately 400 mL of solvents. A thin line of solid formed around the inner surface of the flask. This was swirled back into the remaining solvents; diethyl ether (100 mL) was added, and the mixture was cooled in an ice bath with stirring. After approximately 15 minutes, the mixture was filtered and the collected solids were washed with diethyl ether (100 mL), affording additional product as a yellow solid (1.5 g). Combined yield: 20.7 g, 53.7 mmol, 89%. .sup.1H NMR (500 MHz, D.sub.2O) 4.16 (dd, J=8.9, 5.9 Hz, 1H), 4.11 (dd, half of ABX pattern, J=10.4, 5.8 Hz, 1H), 4.09-4.03 (m, 1H), 3.93 (dd, J=10.3, 3.1 Hz, 1H), 3.61-3.46 (m, 2H), 3.46-3.30 (m, 2H), 2.53-2.36 (m, 4H), 2.06-1.97 (m, 1H), 1.85 (dd, J=14.1, 4.6 Hz, 1H), 1.82-1.72 (m, 2H), 1.72-1.65 (m, 1H), 1.59 (ddd, half of ABXY pattern, J=18, 9, 4.5 Hz, 1H), 1.43 (s, 9H).

(303) Conversion of C94 to C48, for Assessment of Absolute Stereochemistry

(304) A small sample of C94 was derivatized via reaction with benzenesulfonyl chloride and saturated aqueous sodium bicarbonate solution for 1 hour at 40 C. The reaction mixture was extracted with ethyl acetate, and the solvent was removed from the extract under a stream of nitrogen. Supercritical fluid chromatographic analysis (Column: Chiral Technologies Chiralcel OJ-H, 5 m; Mobile phase A: carbon dioxide; Mobile phase B: methanol; Gradient: 5% to 60% B) revealed the product to have an enantiomeric excess of >99%. Injection under the same conditions of samples of C48 and C49 established the derivatization product as identical to C48, the absolute configuration of which was determined via X-ray crystallographic analysis (see above).

Step 3. Synthesis of tert-butyl (3R)-3-[(cyclopropylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C95)

(305) Cyclopropanesulfonyl chloride (56.8 mg, 0.404 mmol) and triethylamine (136 mg, 1.34 mmol) were added to a suspension of C94 (100 mg, 0.26 mmol) in dichloromethane (1 mL) at 16 C. The reaction mixture was stirred at 10 C. for 14 hours, whereupon it was concentrated in vacuo and combined with material from a similar reaction carried out using C94 (30 mg, 78 mol). The resulting mixture was purified via silica gel chromatography (Gradient: 0% to 15% methanol in dichloromethane) to provide the product as a yellow gum. Yield: 90 mg, 0.25 mmol, 74%. LCMS m/z 383.3 [M+Na.sup.+].

Step 4. Synthesis of tert-butyl (3R)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C96)

(306) To a 0 C. suspension of C95 (90 mg, 0.25 mmol) in N,N-dimethylformamide (1 mL) was added sodium hydride (60% dispersion in mineral oil; 20 mg, 0.50 mmol), and the reaction mixture was stirred at 0 C. for 30 minutes. Iodomethane (53.2 mg, 0.375 mmol) was added at 0 C., and the reaction mixture was stirred at 15 C. for 2 hours. It was then treated with saturated aqueous sodium chloride solution (40 mL) and extracted with ethyl acetate (330 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Silica gel chromatography (Gradient: 0% to 50% ethyl acetate in petroleum ether) provided the product as a colorless gum. Yield: 78 mg, 0.21 mmol, 84%. LCMS m/z 397.3 [M+Na.sup.+].

Step 5. Synthesis of (2R)-1, 1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3R)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C97)

(307) Conversion of C96 to the product was carried out using the method described for synthesis of C89 from C88 in Example 92. The product was obtained as a colorless gum. Yield: 67 mg, 0.12 mmol, 57%. LCMS m/z 573.0 [M+Na.sup.+].

Step 6. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3R)-3-[(cyclopropylsulfonyl)(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (93)

(308) Conversion of C97 (67 mg, 0.12 mmol) to the product was carried out using the method described for synthesis of 93 from C91 in Example 93. In this case, purification was effected using reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 35% to 55% B), affording the product as a brown gum. Yield: 10.0 mg, 23.2 mol, 19%. LCMS m/z 431.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.32-5.20 (m, 1H), 4.73-4.63 (m, 1H), 4.04-3.93 (m, 2H), 3.93-3.74 (m, 4H), 3.46-3.24 (m, 2H), 2.88 (s, 3H), 2.55-2.25 (v br s, 1H), 2.30-2.21 (m, 1H), 2.14-2.02 (m, 1H), 1.86-1.68 (m, 4H), 1.56-1.41 (m, 1H), 1.22-1.14 (m, 2H), 1.04-0.96 (m, 2H).

Example 95

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-phenyl-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C101, ENT-2] (95)

(309) ##STR00138##

Step 1. Synthesis of tert-butyl 4-(2,3-dibromopropyl)-4-hydroxypiperidine-1-carboxylate (C98)

(310) This reaction was carried out in two identical batches. A solution of tert-butyl 4-hydroxy-4-(prop-2-en-1-yl)piperidine-1-carboxylate (209 g, 0.866 mol) in dichloromethane (1.2 L) was cooled in a cold water bath. A solution of bromine (152 g, 0.951 mol) in dichloromethane (250 mL) was added at such a rate that the color of the reaction mixture did not become intense. At the conclusion of the addition, an aqueous solution containing sodium thiosulfate and sodium bicarbonate was added to the reaction mixture, and stirring was continued until the mixture had completely decolorized. At this point, the two batches were combined. The aqueous layer was extracted with dichloromethane (3400 mL), and the combined organic layers were washed with saturated aqueous sodium chloride solution (2200 mL), dried over sodium sulfate, and concentrated in vacuo to afford the product as a red gum. Yield: 600 g, 1.5 mol, 87%. .sup.1H NMR (400 MHz, CDCl.sub.3) 4.43-4.33 (m, 1H), 3.96-3.74 (m, 2H), 3.91 (dd, J=10.3, 4.0 Hz, 1H), 3.66 (dd, J=10.0, 9.8 Hz, 1H), 3.27-3.13 (m, 2H), 2.47 (dd, half of ABX pattern, J=15.8, 2.8 Hz, 1H), 2.13 (dd, half of ABX pattern, J=15.7, 8.9 Hz, 1H), 1.78-1.68 (m, 2H), 1.65-1.53 (m, 2H, assumed; partially obscured by water peak), 1.47 (s, 9H).

Step 2. Synthesis of tert-butyl 3-bromo-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C99)

(311) Potassium carbonate (119 g, 861 mmol) was added to a cooled solution of C98 (230 g, 573 mmol) in methanol (1.5 L), and the reaction mixture was stirred at 10 C. to 15 C. for 16 hours. The crude reaction mixture was combined with the crude reaction mixtures from two similar reactions using C98 (350 g, 873 mmol; and 20 g, 50 mmol) and filtered. The filtrate was concentrated in vacuo, and the resulting red oil was recrystallized from petroleum ether (150 mL) at 0 C. to provide a light yellow solid (360 g). This was subjected to silica gel chromatography (Eluent: dichloromethane), and the purified material was recrystallized from petroleum ether (120 mL) and washed with petroleum ether (340 mL) to afford the product as a white solid (180 g). The mother liquors from recrystallization were concentrated under reduced pressure and purified by silica gel chromatography (Gradient: 0% to 20% ethyl acetate in petroleum ether). The resulting material was recrystallized from petroleum ether (100 mL) and washed with petroleum ether (340 mL), affording additional product as a white solid (95 g). Combined yield: 275 g, 0.859 mol, 57%. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 4.71-4.63 (m, 1H), 4.12 (dd, J=10.4, 4.9 Hz, 1H), 3.90 (dd, J=10.5, 3.8 Hz, 1H), 3.52-3.40 (m, 2H), 3.3-3.15 (m, 2H), 2.41 (dd, J=14.3, 7.3 Hz, 1H), 2.10 (dd, J=14.0, 4.0 Hz, 1H), 1.79-1.71 (m, 1H), 1.65 (br ddd, half of ABXY pattern, J=13, 10, 4 Hz, 1H), 1.55-1.41 (m, 2H), 1.39 (s, 9H).

Step 3. Synthesis of tert-butyl 3-phenyl-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, ENT-1 (C100) and tert-butyl 3-phenyl-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, ENT-2 (C101)

(312) A mixture of C99 (150 mg, 0.468 mmol), phenylboronic acid (114 mg, 0.935 mmol), trans-2-aminocyclohexanol (10.8 mg, 93.7 mol) and nickel(II) iodide (29.3 mg, 93.7 mol) in 2-propanol (3 mL, previously dried over molecular sieves) was treated with sodium bis(trimethylsilyl)amide (1 M solution in tetrahydrofuran; 0.937 mL, 0.937 mmol). The reaction vessel was then capped, warmed to 60 C., and stirred for 14 hours. The resulting suspension was combined with a similar reaction mixture carried out using C99 (50 mg, 0.16 mmol), filtered through a pad of diatomaceous earth, and concentrated in vacuo. The residue was purified via chromatography on silica gel (Gradient: 0% to 40% ethyl acetate in petroleum ether) to afford the racemic product as a white solid. Yield: 170 mg, 0.536 mmol, 85%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.36-7.30 (m, 2H), 7.3-7.21 (m, 3H, assumed; partially obscured by solvent peak), 4.23 (dd, J=8, 8 Hz, 1H), 3.80 (dd, J=9, 9 Hz, 1H), 3.70-3.47 (m, 3H), 3.44-3.33 (m, 2H), 2.27 (dd, J=12.5, 8 Hz, 1H), 1.84 (dd, J=12, 11 Hz, 1H), 1.79-1.67 (m, 3H), 1.64-1.55 (m, 1H, assumed; partially obscured by water peak), 1.47 (s, 9H).

(313) The component enantiomers were separated using supercritical fluid chromatography [Column: Chiral Technologies Chiralpak AD, 10 m; Mobile phase: 35% (0.1% ammonium hydroxide in methanol) in carbon dioxide]. The first-eluting enantiomer was assigned as C100. Yield: 65 mg, 38% for the separation. LCMS m/z 262.1 [(M-2-methylprop-1-ene)+H].sup.+. The second-eluting enantiomer was assigned as C101. Yield: 70 mg, 41% for the separation. LCMS m/z 262.1 [(M2-methylprop-1-ene)+H].sup.+.

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-phenyl-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C101, ENT-2] (C102)

(314) Trifluoroacetic acid (0.6 mL) was added drop-wise to a solution of C101 (70.0 mg, 0.220 mmol) in dichloromethane (2 mL), and the reaction mixture was stirred at 25 C. for 2 hours. Volatiles were removed under reduced pressure to provide 3-phenyl-1-oxa-8-azaspiro[4.5]decane, trifluoroacetate salt, as a yellow gum. This material was dissolved in acetonitrile (2 mL), cooled to 0 C., and slowly treated with triethylamine (89.5 mg, 0.884 mmol). After this solution had stirred for 30 minutes, C2 (reaction solution in acetonitrile containing 0.221 mmol) was added at 0 C. The reaction mixture was stirred at 25 C. for 18 hours, whereupon it was concentrated in vacuo and purified by preparative thin layer chromatography on silica gel (Eluent: 3:1 petroleum ether/ethyl acetate), affording the product as a yellow gum (120 mg). This material was taken directly to the following step. LCMS m/z 516.1 [M+Na.sup.+].

Step 5. Synthesis of (2R)-1, 1,1-trifluoro-3-hydroxypropan-2-yl 3-phenyl-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C101, ENT-2] (95)

(315) Trifluoroacetic acid (0.5 mL) was added to a 0 C. solution of C102 (from the previous step; 120 mg, 50.220 mmol) in dichloromethane (1.5 mL). The reaction mixture was stirred at 25 C. for 2 hours, whereupon it was concentrated in vacuo and subjected to preparative thin layer chromatography on silica gel (Eluent: 3:1 petroleum ether/ethyl acetate). The material obtained (40 mg) was then purified using reversed phase HPLC (Column: Daiso C18, 5 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 42% to 72% B) to afford the product as a colorless gum. Yield: 10.1 mg, 27.0 mol, 12% over 2 steps. LCMS m/z 373.9 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.36-7.30 (m, 2H), 7.27-7.22 (m, 3H), 5.32-5.21 (m, 1H), 4.24 (dd, J=8.0, 8.0 Hz, 1H), 4.01 (dd, half of ABX pattern, J=12.4, 2.9 Hz, 1H), 3.92-3.74 (m, 4H), 3.60-3.35 (m, 3H), 2.32-2.22 (m, 1H), 1.92-1.55 (m, 5H, assumed; partially obscured by water peak).

Example 96

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-phenyl-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C100, ENT-1] (96)

(316) ##STR00139##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-phenyl-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C100, ENT-1] (C103)

(317) Trifluoroacetic acid (2 mL) was added to a 0 C. suspension of C100 (65 mg, 0.20 mmol) in dichloromethane (3 mL). The reaction mixture was stirred at 18 C. for 2 hours, whereupon it was concentrated in vacuo to provide the deprotected material as a yellow gum. The gum was dissolved in acetonitrile (1 mL), cooled to 0 C., and treated with C2 (reaction solution in acetonitrile containing 0.24 mmol) and triethylamine (166 mg, 1.64 mmol). This reaction mixture was stirred at 18 C. for 16 hours, and then treated with additional C2 (reaction solution in acetonitrile containing 0.24 mmol). Stirring was continued at 18 C. for an additional 16 hours. Volatiles were removed under reduced pressure, and the residue was subjected to chromatography on silica gel (Gradient: 0% to 100% ethyl acetate in petroleum ether) to afford the product as a yellow gum (101 mg). This material was used in the following step without additional purification. LCMS m/z 516.1 [M+Na.sup.+]

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-phenyl-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C100, ENT-1] (96)

(318) Trifluoroacetic acid (2 mL) was added to a 0 C. suspension of C103 (from the previous step; 0.20 mmol) in dichloromethane (2 mL), and the reaction mixture was stirred at 20 C. for 1 hour. After the reaction mixture had been cooled to 0 C., aqueous sodium bicarbonate solution (40 mL) was slowly added, and the purple mixture became colorless. It was extracted with dichloromethane (320 mL), and the combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Silica gel chromatography (Gradient: 0% to 50% ethyl acetate in petroleum ether) was followed by reversed phase HPLC (Column: Agela Durashell, 5 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 5% to 95% B), affording the product as a yellow oil. Yield: 15.2 mg, 40.7 mol, 20% over 2 steps. LCMS m/z 373.9 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.36-7.30 (m, 2H), 7.28-7.21 (m, 3H), 5.32-5.21 (m, 1H), 4.24 (dd, J=8.3, 7.8 Hz, 1H), 4.06-3.97 (m, 1H), 3.93-3.74 (m, 4H), 3.60-3.32 (m, 3H), 2.49-2.38 (m, 1H), 2.27 (dd, J=12.6, 8.3 Hz, 1H), 1.89-1.6 (m, 4H), 1.87 (dd, J=12.0, 10.8 Hz, 1H).

Example 97

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-(5-fluoropyridin-2-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, trifluoroacetate salt (97)

(319) ##STR00140##

Step 1. Synthesis of 3-bromo-1-oxa-8-azaspiro[4.5]decane, trifluoroacetate salt (C104)

(320) Trifluoroacetic acid (100 mL) was added drop-wise to a 0 C. solution of C99 (25.0 g, 78.1 mmol) in dichloromethane (400 mL). After the reaction mixture had been stirred at 13 C. for 15 hours, it was concentrated in vacuo to afford the product as a brown oil (30 g). This material was used in the next step without additional purification. .sup.1H NMR (400 MHz, CD.sub.3OD) 4.63-4.55 (m, 1H), 4.20 (dd, half of ABX pattern, J=10.5, 4.5 Hz, 1H), 4.04 (dd, half of ABX pattern, J=10.5, 3.5 Hz, 1H), 3.3-3.21 (m, 4H), 2.50 (dd, half of ABX pattern, J=14.6, 7.0 Hz, 1H), 2.30-2.18 (m, 2H), 1.97 (ddd, J=14, 10, 6.5 Hz, 1H), 1.91-1.77 (m, 2H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-bromo-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C105)

(321) Triethylamine (39.5 g, 390 mmol) was added to a 15 C. solution of C104 (from the previous step; 30 g, 578.1 mmol) in acetonitrile (400 mL). The resulting solution was stirred at 15 C. for 1 hour, whereupon it was cooled to 0 C. and treated with C2 [reaction solution in acetonitrile (400 mL) containing 85.9 mmol]. After the reaction mixture had been stirred at 13 C. for 15 hours, it was concentrated in vacuo and purified twice via chromatography on silica gel (Gradient: 5% to 9% ethyl acetate in petroleum ether). A final silica gel chromatographic purification (Gradient: 0% to 9% ethyl acetate in petroleum ether) afforded the product as a colorless gum. Yield: 20.3 g, 40.9 mmol, 52% over 2 steps. LCMS m/z 519.8 (bromine isotope pattern observed) [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.25 (d, J=8.5 Hz, 2H), 6.89 (d, J=8.7 Hz, 2H), 5.54-5.43 (m, 1H), 4.51 (AB quartet, upfield doublet is broadened, J.sub.AB=11.7 Hz, .sub.AB=29.1 Hz, 2H), 4.44-4.36 (m, 1H), 4.19 (dd, J=10.4, 5.3 Hz, 1H), 4.07-3.99 (m, 1H), 3.91-3.63 (m, 4H), 3.82 (s, 3H), 3.44-3.27 (m, 2H), 2.42-2.25 (m, 1H), 2.24-2.08 (m, 1H), 2.04-1.89 (m, 1H), 1.81-1.47 (m, 3H).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-(4,4, 5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C106)

(322) A mixture of C105 (6.50 g, 13.1 mmol), 4,4,4,4,5,5,5,5-octamethyl-2,2-bi-1,3,2-dioxaborolane (4.99 g, 19.6 mmol), polymer-bound triphenylphosphine (687 mg, 2.62 mmol), lithium methoxide (995 mg, 26.2 mmol), and copper(I) iodide (249 mg, 1.31 mmol) in N,N-dimethylformamide (50 mL) was stirred at 1 C. to 10 C. for 16 hours. The reaction mixture was then diluted with dichloromethane (150 mL) and filtered; the filter cake was washed with dichloromethane (150 mL), and the combined filtrates were concentrated in vacuo. The resulting oil was mixed with saturated aqueous ammonium chloride solution (150 mL) and extracted with diethyl ether (3150 mL). The combined organic layers were washed sequentially with water (150 mL) and saturated aqueous sodium chloride solution (150 mL), dried over sodium sulfate, filtered, and concentrated under reduced pressure to provide the product as a pale yellow gum. Yield: 7.00 g, 12.9 mmol, 98%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.24 (br d, J=8.8 Hz, 2H), 6.88 (br d, J=8.8 Hz, 2H), 5.53-5.42 (m, 1H), 4.51 (AB quartet, J.sub.AB=11.7 Hz, .sub.AB=27.2 Hz, 2H), 4.03 (dd, J=8.3, 8.2 Hz, 1H), 3.81 (s, 3H), 3.80-3.63 (m, 5H), 3.45-3.30 (m, 2H), 1.98-1.74 (m, 2H), 1.72-1.40 (m, 5H), 1.25 (s, 12H).

Step 4. Synthesis of potassium trifluoro{8-[({(2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl}oxy) carbonyl]-1-oxa-8-azaspiro[4.5]dec-3-yl}borate(1-) (C107)

(323) Aqueous potassium hydrogenfluoride solution (4.5 M, 11.5 mL, 51.8 mmol) was added to a 0 C. solution of C106 (7.00 g, 12.9 mmol) in tetrahydrofuran (50 mL) and the reaction mixture was stirred at 0 C. to 5 C. for 16 hours. Removal of volatiles in vacuo provided a thick oil, which was extracted with acetone (475 mL). The combined acetone layers were filtered, and the filtrate was concentrated to a volume of approximately 20 mL, cooled to 0 C., and diluted with diethyl ether (150 mL). A white tacky material appeared; the solvent was removed via decantation, and the remaining gum was triturated with diethyl ether (150 mL) to afford the product as a white solid. Yield: 3.8 g, 7.26 mmol, 56%. LCMS m/z 483.9 [M-]. .sup.1H NMR (400 MHz, acetone-d.sub.6) 7.27 (br d, J=8.7 Hz, 2H), 6.91 (br d, J=8.7 Hz, 2H), 5.55-5.43 (m, 1H), 4.52 (AB quartet, J.sub.AB=11.6 HZ, .sub.AB=19.0 Hz, 2H), 3.84-3.70 (m, 3H), 3.79 (s, 3H), 3.70-3.53 (m, 3H), 3.44-3.23 (m, 2H), 1.70-1.58 (m, 1H), 1.58-1.45 (m, 4H), 1.45-1.34 (m, 1H), 1.30-1.14 (m, 1H).

Step 5. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-(5-fluoropyridin-2-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, trifluoroacetate salt (97)

(324) A mixture of 2-bromo-5-fluoropyridine (17.6 mg, 0.100 mmol), C107 (78.3 mg, 0.150 mmol), [Ir{dFCF.sub.3ppy}.sub.2(bpy)] PF.sub.6 (2.5 mg, 2.4 mol), cesium carbonate (48.9 mg, 0.150 mmol), nickel(II) chloride, 1,2-dimethoxyethane adduct (1.1 mg, 5.0 mol), and 4,4-di-tert-butyl-2,2-bipyridine (1.4 mg, 5.2 mol) was degassed under vacuum and then purged with nitrogen; this evacuation-purge cycle was carried out a total of three times. 1,4-Dioxane (7 mL) was added, and the reaction mixture was sonicated and shaken to provide a suspension. The reaction mixture was then irradiated with blue visible light (wavelength: 460 nm) from a 60 watt blue LED strip for 18 hours. After removal of volatiles in vacuo, a mixture of dichloromethane (0.5 mL) and trifluoroacetic acid (0.5 mL) was added, and the resulting reaction mixture was stirred at room temperature for 30 minutes. The reaction mixture was concentrated in vacuo, and the residue was purified via reversed phase HPLC (Column: Waters Sunfire C18, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 10% to 100% B). The product was assumed to be a mixture of two diastereomers. Yield: 1.4 mg, 2.7 mol, 3%. LCMS m/z 393.3 [M+H].sup.+. Retention time: 2.96 minutes [Analytical HPLC conditionsColumn: Waters Atlantis dC18, 4.650 mm, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 5.0% to 95% B, linear over 4.0 minutes Flow rate: 2 mL/minute].

Example 98

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 2-(2-fluorobenzoyl)-2, 8-diazaspiro[4.5]decane-8-carboxylate (98)

(325) ##STR00141##

Step 1. Synthesis of tert-butyl 2-(2-fluorobenzoyl)-2, 8-diazaspiro[4.5]decane-8-carboxylate (C108)

(326) To a suspension of tert-butyl 2,8-diazaspiro[4.5]decane-8-carboxylate (200 mg, 0.832 mmol) in acetonitrile (2 mL) were added 2-fluorobenzoic acid (175 mg, 1.25 mmol), O-(7-azabenzotriazol-1-yl)-N,N,N,N-tetramethyluronium hexafluorophosphate (HATU; 506 mg, 1.33 mmol), and N,N-diisopropylethylamine (323 mg, 2.50 mmol). The reaction mixture was stirred at 25 C. for 16 hours, whereupon it was concentrated in vacuo. The residue was dissolved in methanol (8 mL), treated with ion exchange resin Amberlyst A26, hydroxide form [3.6 g, pre-washed with methanol (7 mL)], stirred at 25 C. for 1 hour, and filtered. The filtrate was concentrated under reduced pressure and subjected to silica gel chromatography (Gradient: 0% to 50% ethyl acetate in petroleum ether), affording the product as a colorless gum. By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. Yield: 231 mg, 0.637 mmol, 77%. .sup.1H NMR (400 MHz, CD.sub.3OD) 7.55-7.46 (m, 1H), 7.45-7.38 (m, 1H), 7.32-7.26 (m, 1H), 7.26-7.18 (m, 1H), 3.72-3.66 (m, 1H), 3.56-3.47 (m, 1H), 3.51 (s, 1H), 3.46-3.3 (m, 4H), 3.21 (br s, 1H), [1.94 (dd, J=7.5, 7.3 Hz) and 1.87 (dd, J=7.3, 7.0 Hz), total 2H], 1.66-1.48 (m, 4H), [1.47 (s) and 1.43 (s), total 9H].

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 2-(2-fluorobenzoyl)-2, 8-diazaspiro[4.5]decane-8-carboxylate (C109)

(327) Conversion of C108 to the product was carried out using the method described for synthesis of C89 from C88 in Example 92. The product was obtained as a colorless gum. Yield: 500 mg, 0.93 mmol, quantitative. LCMS m/z 539.1 [M+H].sup.+.

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 2-(2-fluorobenzoyl)-2, 8-diazaspiro[4.5]decane-8-carboxylate (98)

(328) Conversion of C109 to the product was carried out using the method described for synthesis of 92 from C89 in Example 92. In this case, the gradient used for HPLC purification was 36% to 56% B, and the product was isolated as a colorless gum. By .sup.1H NMR analysis, this was judged to be a mixture of rotamers. Yield: 89 mg, 0.21 mmol, 23%. LCMS m/z 419.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.46-7.37 (m, 2H), 7.25-7.19 (m, 1H), 7.15-7.08 (m, 1H), 5.32-5.19 (m, 1H), 4.04-3.94 (m, 1H), 3.92-3.79 (m, 1H), 3.79-3.62 (m, 2H), 3.58 (s, 1H), 3.56-3.30 (m, 4H), 3.20 (s, 1H), 2.6-2.3 (br m, 1H), [1.90 (dd, J=7.5, 7.3 Hz) and 1.82 (dd, J=7.0, 7.0 Hz), total 2H], 1.74-1.47 (m, 4H, assumed; partially obscured by water peak).

Examples 99 and 100

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-[benzoyl(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C112, DIAST-2] (99) and (2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-[benzoyl(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C111, DIAST-1] (100)

(329) ##STR00142##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-(benzoylamino)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C110)

(330) A solution of benzoyl chloride (58.5 mg, 0.416 mmol) in dichloromethane (0.5 mL) was added to a 0 C. solution of C73 (150 mg, 0.347 mmol) and triethylamine (105 mg, 1.04 mmol) in dichloromethane (2 mL). The reaction mixture was stirred at 25 C. for 3 hours, whereupon saturated aqueous ammonium chloride solution (2 mL) was added, and the resulting mixture was extracted with dichloromethane (23 mL). The combined organic layers were washed with saturated aqueous sodium chloride solution (23 mL), filtered, and concentrated in vacuo. Silica gel chromatography (Gradient: 5% to 20% ethyl acetate in petroleum ether) provided the product as a colorless gum. Yield: 135 mg, 0.252 mmol, 73%. LCMS m/z 559.1 [M+Na.sup.+].

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-[benzoyl(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, DIAST-1 (C111) and (2R)-1,1, 1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-[benzoyl(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, DIAST-2 (C112)

(331) Sodium hydride (60% dispersion in mineral oil; 17.1 mg, 0.428 mmol) was added to a 0 C. solution of C110 (115 mg, 0.214 mmol) in dry tetrahydrofuran (2 mL), and the reaction mixture was stirred at 0 C. for 30 minutes. Iodomethane (45.6 mg, 0.321 mmol) was added, and stirring was continued at 25 C. for 2 hours. The reaction mixture was then combined with a similar reaction mixture using C110 (20 mg, 37 mol) and cooled to 0 C. Saturated aqueous ammonium chloride solution (5 mL) was added, and the resulting mixture was extracted with ethyl acetate (25 mL). The combined organic layers were washed with saturated aqueous sodium chloride solution (25 mL), filtered, and concentrated in vacuo to afford the product, a diastereomeric mixture of C111 and C112, as a colorless gum. Yield of diastereomeric product mixture: 130 mg, 0.236 mmol, 94%. LCMS m/z 573.2 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3), characteristic peaks: 7.46-7.40 (m, 3H), 7.40-7.34 (m, 2H), 7.24 (br d, J=8.5 Hz, 2H), 6.88 (br d, J=8.7 Hz, 2H), 5.53-5.42 (m, 1H), 4.51 (AB quartet, J.sub.AB=11.7 Hz, .sub.AB=28.4 Hz, 2H), 3.91-3.85 (m, 1H), 3.85-3.63 (m, 3H), 3.82 (s, 3H), 3.42-3.19 (m, 2H), 3.07-2.89 (m, 3H), 1.85-1.67 (m, 3H).

(332) The component diastereomers were separated via supercritical fluid chromatography (Column: Chiral Technologies Chiralpak IC, 10 m; Mobile phase: 40% (0.1% ammonium hydroxide in 2-propanol) in carbon dioxide]. The first-eluting diastereomer was C111 (50 mg) and the second-eluting diastereomer was C112 (55 mg).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-[benzoyl(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C112, DIAST-2] (99)

(333) Trifluoroacetic acid (0.5 mL) was added to a solution of C112 (55 mg, 0.10 mmol) in dichloromethane (1 mL), and the reaction mixture was stirred at 18 C. for 2 hours. Saturated aqueous sodium bicarbonate solution was added until the pH reached 89, and the resulting mixture was extracted with dichloromethane (22 mL). The combined organic layers were washed with saturated aqueous sodium chloride solution, filtered, dried over sodium sulfate, and concentrated in vacuo. Reversed phase HPLC (Column: Agela Durashell, 5 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 8% to 58% B) afforded the product as a white solid. Yield: 15.6 mg, 36.2 mol, 36%. LCMS m/z 431.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3), characteristic peaks: 7.46-7.34 (m, 5H), 5.30-5.20 (m, 1H), 4.04-3.96 (m, 1H), 3.92-3.68 (m, 4H), 3.44-3.15 (m, 2H), 3.07-2.89 (m, 3H), 2.46-1.96 (m, 2H), 1.87-1.72 (m, 3H).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-[benzoyl(methyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C111, DIAST-1] (100)

(334) Conversion of C111 to the product was effected using the method employed for synthesis of 99 from C112. Yield: 17.4 mg, 40.4 mol, 44%. LCMS m/z 431.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3), characteristic peaks: 7.46-7.40 (m, 3H), 7.40-7.34 (m, 2H), 5.30-5.20 (m, 1H), 4.06-3.95 (m, 1H), 3.94-3.70 (m, 4H), 3.48-3.21 (m, 2H), 3.08-2.88 (m, 3H), 2.43-2.27 (m, 1H), 1.88-1.72 (m, 3H).

Example 101

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-(1, 1-dioxido-1, 2-benzothiazol-2(3H)-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (101)

(335) ##STR00143##

Step 1. Synthesis of 2,3-dihydro-1,2-benzothiazole 1,1-dioxide (C113)

(336) A solution of 1,2-benzothiazol-3(2H)-one 1,1-dioxide (200 mg, 1.09 mmol) in tetrahydrofuran (2 mL) was added drop-wise to a 0 C. suspension of lithium aluminum hydride (45.6 mg, 1.20 mmol) in tetrahydrofuran (3 mL). After the reaction mixture had stirred for 30 minutes at 0 C., it was gradually warmed to 15 C. and stirred at 15 C. for 16 hours. The white suspension was treated with saturated aqueous ammonium chloride solution, and then extracted with ethyl acetate (20 mL). The organic layer was dried over sodium sulfate, filtered, and concentrated in vacuo to provide the product as a grey solid. Yield: 160 mg, 0.946 mmol, 87%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.81 (d, J=7.8 Hz, 1H), 7.63 (dd, half of ABX pattern, J=7.5, 7.3 Hz, 1H), 7.54 (dd, half of ABX pattern, J=7.5, 7.5 Hz, 1H), 7.41 (d, J=7.8 Hz, 1H), 4.95-4.80 (br s, 1H), 4.55 (s, 2H).

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-(1,1-dioxido-1,2-benzothiazol-2(3H)-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C114)

(337) A mixture of C105 (80 mg, 0.16 mmol), C113 (39.3 mg, 0.232 mmol), cesium carbonate (114 mg, 0.350 mmol), and potassium iodide (28.9 mg, 0.174 mmol) in N,N-dimethylformamide (2 mL) was stirred at 80 C. for 16 hours. The reaction mixture was then diluted with ethyl acetate (30 mL), washed with saturated aqueous sodium chloride solution (330 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. Preparative thin layer chromatography on silica gel (Eluent: 1:3 ethyl acetate/petroleum ether) provided the product as a light yellow oil. Yield: 55 mg, 94 mol, 59%. LCMS m/z 607.0 [M+Na.sup.+].

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-(1,1-dioxido-1,2-benzothiazol-2(3H)-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (101)

(338) Trifluoroacetic acid (2 mL) was added in a drop-wise manner to a 0 C. solution of C114 (55 mg, 94 mol) in dichloromethane (6 mL). The reaction mixture was stirred at 0 C. for 1 hour, whereupon it was diluted with saturated aqueous sodium bicarbonate (30 mL) and extracted with ethyl acetate (30 mL). The organic layer was dried over sodium sulfate, filtered, concentrated in vacuo, and purified via reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 30% to 50% B). The product was obtained as a white solid, presumed to be a mixture of diastereomers. Yield: 6.0 mg, 13 mol, 14%. LCMS m/z 487.0 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.81 (br d, J=7.5 Hz, 1H), 7.64 (ddd, J=7.5, 7.5, 1.2 Hz, 1H), 7.56 (br dd, J=7, 7 Hz, 1H), 7.42 (br d, J=7.8 Hz, 1H), 5.31-5.21 (m, 1H), 4.41 (br AB quartet, J.sub.AB=14 Hz, .sub.AB=12 Hz, 2H), 4.4-4.30 (m, 1H), 4.16 (dd, half of ABX pattern, J=9.7, 6.4 Hz, 1H), 4.05 (dd, half of ABX pattern, J=9.8, 5.5 Hz, 1H), 4.05-3.96 (m, 1H), 3.93-3.73 (m, 3H), 3.50-3.28 (m, 2H), 2.42-2.25 (m, 2H), 2.21-2.08 (m, 1H), 1.89-1.70 (m, 3H).

Example 102

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-[(5-methylpyridin-2-yl)methyl]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (102)

(339) ##STR00144##

Step 1. Synthesis of tert-butyl 3-methylidene-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C115)

(340) Methyltriphenylphosphonium bromide (8.4 g, 24 mmol) was added portion-wise to a mixture of sodium hydride (60% dispersion in mineral oil; 940 mg, 23.5 mmol) in dimethyl sulfoxide (40 mL), and the reaction mixture was stirred for 30 minutes at room temperature. A solution of tert-butyl 3-oxo-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (2.0 g, 7.8 mmol) in dimethyl sulfoxide (18 mL) was then added drop-wise, and the reaction mixture was allowed to continue stirring at room temperature for 72 hours. The reaction was then carefully quenched with water (250 mL), and extracted with diethyl ether (550 mL). The combined organic layers were washed with water (225 mL), dried over magnesium sulfate, filtered, and concentrated in vacuo. The residue was triturated three times with heptane to afford an off-white solid, which proved to be largely triphenylphosphine oxide on analysis. The combined heptane portions from the triturations were concentrated in vacuo and subjected to silica gel chromatography (Eluents: 0%, followed by 10% and 20% ethyl acetate in heptane), which afforded the product as a colorless oil. Yield: 1.77 g, 6.99 mmol, 90%. GCMS m/z 253.1 [M+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.02-4.98 (m, 1H), 4.95-4.91 (m, 1H), 4.37-4.33 (m, 2H), 3.60 (ddd, J=13, 5, 5 Hz, 2H), 3.34 (ddd, J=13.3, 9.9, 3.3 Hz, 2H), 2.42-2.38 (m, 2H), 1.70-1.63 (m, 2H), 1.55 (ddd, J=13.3, 10.0, 4.5 Hz, 2H), 1.46 (s, 9H).

Step 2. Synthesis of tert-butyl 3-[(5-methylpyridin-2-yl)methyl]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C116)

(341) Compound C115 (200 mg, 0.789 mmol) was dissolved in a 9-borabicyclo[3.3.1]nonane solution (0.5 M in tetrahydrofuran; 1.58 mL, 0.79 mmol). After the reaction vessel had been capped, the reaction mixture was stirred at 70 C. for 1 hour, whereupon it was cooled to room temperature and added to a mixture of 2-bromo-5-methylpyridine (123 mg, 0.715 mmol), [1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II), dichloromethane complex (32 mg, 39 mol), and potassium carbonate (109 mg, 0.789 mmol) in a mixture of N,N-dimethylformamide (1.7 mL) and water (170 L). The reaction vessel was capped and stirred at 60 C. overnight. After the reaction mixture had cooled to room temperature, it was poured into water and extracted three times with ethyl acetate. The combined organic layers were washed with saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered, and concentrated in vacuo. The residue was purified via silica gel chromatography (Eluents: 10%, followed by 25%, 50%, and 75% ethyl acetate in heptane) to afford the product as a colorless oil. Yield: 91 mg, 0.26 mmol, 36%. LCMS m/z 347.3 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.39-8.35 (m, 1H), 7.44 (br d, J=7 Hz, 1H), 7.04 (d, J=7.7 Hz, 1H), 3.95 (dd, J=8.6, 6.6 Hz, 1H), 3.61-3.47 (m, 2H), 3.55 (dd, J=8.5, 7.8 Hz, 1H), 3.40-3.26 (m, 2H), 2.92-2.75 (m, 3H), 2.32 (s, 3H), 1.92 (dd, J=12.5, 7.3 Hz, 1H), 1.7-1.5 (m, 4H), 1.51-1.41 (m, 1H), 1.45 (s, 9H).

Step 3. Synthesis of 3-[(5-methylpyridin-2-yl)methyl]-1-oxa-8-azaspiro[4.5]decane, trifluoroacetate salt (C117)

(342) A solution of C116 (91 mg, 0.26 mmol) in dichloromethane (3 mL) was cooled to 0 C. Trifluoroacetic acid (1.5 mL) was added, and the reaction mixture was stirred at room temperature for 1 hour. Solvents were removed under reduced pressure to provide the product as a pale yellow oil (185 mg), which was used directly in the following step. GCMS m/z 246.1 [M+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.65-8.62 (br s, 1H), 8.17 (br d, J=8 Hz, 1H), 7.60 (d, J=8.1 Hz, 1H), 3.99 (dd, J=8.8, 7.0 Hz, 1H), 3.58 (dd, J=8.6, 8.2 Hz, 1H), 3.40-3.26 (m, 4H), 3.25 (dd, half of ABX pattern, J=14.4, 7.0 Hz, 1H), 3.13 (dd, half of ABX pattern, J=14.3, 8.3 Hz, 1H), 2.90-2.77 (m, 1H), 2.58 (s, 3H), 2.11-1.80 (m, 5H), 1.63-1.54 (m, 1H, assumed; partially obscured by water peak).

Step 4. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-[(5-methylpyridin-2-yl)methyl]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C118)

(343) Bis(pentafluorophenyl) carbonate (132 mg, 0.335 mmol) was added to a 0 C. solution of C1 (84 mg, 0.34 mmol) in acetonitrile (5 mL). Triethylamine (180 L, 1.29 mmol) was then added, and the reaction mixture was stirred at room temperature for 1 hour. In a separate flask, a solution of C117 (from the previous step; 185 mg, 50.26 mmol) in acetonitrile (3 mL) was cooled to 0 C. and treated with triethylamine (360 L, 2.6 mmol); after this mixture had stirred in the ice bath for a few minutes, the carbonate solution prepared from C1 was added drop-wise to the solution containing C117. The reaction mixture was stirred at 0 C. for a few minutes, and then allowed to stir at room temperature overnight. It was then concentrated in vacuo, and the resulting oil was taken up in ethyl acetate and washed sequentially with aqueous 1 M hydrochloric acid, saturated aqueous sodium bicarbonate solution, and saturated aqueous sodium chloride solution. The organic layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure. Silica gel chromatography (Eluents: 10%, followed by 25%, 50%, and 75% ethyl acetate in heptane) afforded the product as a colorless oil. Yield: 93 mg, 0.18 mmol, 69% over two steps. LCMS m/z 523.4 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.38-8.35 (m, 1H), 7.42 (br dd, J=7.8, 1.8 Hz, 1H), 7.24 (br d, J=8.6 Hz, 2H), 7.02 (d, J=7.9 Hz, 1H), 6.88 (br d, J=8.5 Hz, 2H), 5.53-5.41 (m, 1H), 4.50 (AB quartet, upfield doublet is broadened, J.sub.AB=11.7 Hz, .sub.AB=26.8 Hz, 2H), 4.00-3.92 (m, 1H), 3.81 (s, 3H), 3.79-3.62 (m, 4H), 3.59-3.51 (m, 1H), 3.44-3.27 (m, 2H), 2.90-2.75 (m, 3H), 2.32 (s, 3H), 1.96-1.83 (m, 1H), 1.74-1.38 (m, 5H).

Step 5. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-[(5-methylpyridin-2-yl)methyl]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (102)

(344) Trifluoroacetic acid (2.5 mL) was added portion-wise to a 0 C. solution of C118 (93 mg, 0.18 mmol) in dichloromethane (5 mL). The reaction mixture was allowed to stir at room temperature for 75 minutes, whereupon it was concentrated in vacuo and partitioned between dichloromethane and saturated aqueous sodium bicarbonate solution. The organic layer was extracted twice with dichloromethane, and the combined organic layers were washed with saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered, and concentrated under reduced pressure. Chromatography on silica gel (Eluents: 50%, followed by 100% ethyl acetate in heptane) provided the product as a colorless oil, presumed to be a mixture of diastereomers. Yield: 54 mg, 0.13 mmol, 72%. LCMS m/z 403.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.38-8.34 (m, 1H), 7.43 (br dd, J=7.8, 2.0 Hz, 1H), 7.03 (d, J=7.8 Hz, 1H), 5.30-5.18 (m, 1H), 4.03-3.91 (m, 2H), 3.85 (dd, half of ABX pattern, J=12.3, 6.8 Hz, 1H), 3.82-3.62 (m, 2H), 3.59-3.51 (m, 1H), 3.48-3.25 (m, 2H), 2.90-2.72 (m, 3H), 2.31 (s, 3H), 1.95-1.86 (m, 1H), 1.75-1.59 (m, 3H), 1.56-1.41 (m, 2H).

Examples 103 and 104

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 3-(1H-pyrazol-1-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C120, DIAST-2] (103) and (2R)-1, 1,1-Trifluoro-3-hydroxypropan-2-yl 3-(1H-pyrazol-1-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C119, DIAST-1] (104)

(345) ##STR00145##

Step 1. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-(1H-pyrazol-1-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, DIAST-1 (C119) and (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-(1H-pyrazol-1-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate, DIAST-2 (C120)

(346) A mixture of C105 (200 mg, 0.403 mmol), 1H-pyrazole (54.9 mg, 0.806 mmol), and cesium carbonate (394 mg, 1.21 mmol) in N,N-dimethylformamide (6 mL) was stirred at 20 C. for 16 hours. The reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (320 mL); the combined organic layers were washed with water (310 mL) and with saturated aqueous sodium chloride solution (310 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. Silica gel chromatography (Eluents: 0%, then 10%, then 25% ethyl acetate in petroleum ether) provided a mixture of diastereomeric products C119 and C120 as a colorless oil. Yield: 60 mg, 0.124 mmol, 31%. This material was combined with the diastereomeric product mixture (30 mg) from a similar reaction carried out on C105, and subjected to separation via supercritical fluid chromatography (Column: Chiral Technologies Chiralpak AD, 10 m; Mobile phase: 2:3 (0.1% ammonium hydroxide in methanol)/carbon dioxide). The first-eluting diastereomer was assigned as C119, and the second-eluting diastereomer as C120. Both were obtained as colorless oils. C119: Yield: 43 mg, 48% for the separation. LCMS m/z 506.1 [M+Na.sup.+]. C120: Yield: 38 mg, 42% for the separation. LCMS m/z 506.1 [M+Na.sup.+].

Step 2. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-(1H-pyrazol-1-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C120, DIAST-2] (103)

(347) To a 0 C. solution of C120 (38 mg, 78 mol) in dichloromethane (4 mL) was added trifluoroacetic acid (1 mL), and the reaction mixture was stirred for 1 hour. After solvents had been removed in vacuo, the residue was partitioned between dichloromethane (10 mL) and saturated aqueous sodium bicarbonate solution (20 mL). The aqueous layer was extracted with ethyl acetate (210 mL), and the combined organic layers were dried over sodium sulfate, filtered, and concentrated under reduced pressure. Reversed phase HPLC (Column: Phenomenex Synergi C18, 4 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 19% to 49% B) provided the product as a brown gum. Yield: 17.0 mg, 46.7 mol, 60%. LCMS m/z 363.8 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.54 (d, J=1.5 Hz, 1H), 7.50 (d, J=2.1 Hz, 1H), 6.28 (dd, J=2, 2 Hz, 1H), 5.30-5.21 (m, 1H), 5.05-4.97 (m, 1H), 4.26-4.17 (m, 2H), 4.01 (br dd, J=12.5, 3 Hz, 1H), 3.92-3.73 (m, 3H), 3.50-3.31 (m, 2H), 2.38-2.25 (m, 2H), 1.94-1.56 (m, 4H, assumed; partially obscured by water peak).

Step 3. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl 3-(1H-pyrazol-1-yl)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate [From C119, DIAST-1] (104)

(348) Conversion of C119 to the product was effected using the method described for synthesis of 103 from C120. In this case, the reversed phase HPLC was carried out using a gradient of 37% to 57% B, to provide the product as a brown gum. Yield: 18.2 mg, 50.0 mol, 56%. LCMS m/z 363.8 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3), characteristic peaks: 7.55 (d, J=1.5 Hz, 1H), 7.50 (br s, 1H), 6.28 (br s, 1H), 5.31-5.20 (m, 1H), 5.05-4.96 (m, 1H), 4.26-4.16 (m, 2H), 4.05-3.97 (m, 1H), 3.93-3.74 (m, 3H), 3.49-3.30 (m, 2H), 2.39-2.25 (m, 2H).

Example 105

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl 2-(phenylsulfonyl)-2, 8-diazaspiro[4.5]decane-8-carboxylate (105)

(349) ##STR00146##

(350) A solution of tert-butyl 2,8-diazaspiro[4.5]decane-8-carboxylate (36 mg, 0.15 mmol) in pyridine (0.4 mL) was added to a solution of benzenesulfonyl chloride (39.7 mg, 0.225 mmol) and N,N-dimethylpyridin-4-amine (0.25 mg, 2.0 mol) in pyridine (0.4 mL), and the reaction mixture was shaken at room temperature for 2 days. The pyridine was removed in vacuo, and the residue was partitioned between half-saturated aqueous sodium bicarbonate solution (1.5 mL) and ethyl acetate (2.4 mL). After the mixture had been vortexed, the organic layer was eluted through a solid phase extraction cartridge (6 mL) charged with sodium sulfate (1 g); this extraction procedure was repeated twice, and the combined eluents were concentrated in vacuo. The residue was treated with a mixture of 1,2-dichloroethane and trifluoroacetic acid (1:1; 1 mL), shaken at room temperature for 2.5 hours, and concentrated under reduced pressure. The remaining material was dissolved in 1,2-dichloroethane (2.4 mL), vortexed, and loaded onto an SCX (strong cation exchanger) solid phase extraction cartridge (Silicycle, 6 mL, 1 g); the vial was rinsed with a mixture of methanol and 1,2-dichloroethane (1:1; 22.4 mL). The cartridge was eluted with methanol (5 mL), followed by a solution of triethylamine in methanol (1 M, 7.5 mL) to elute the deprotected intermediate. Fractions containing the desired material were concentrated in vacuo, and the residue was azeotroped with toluene (21 mL) to remove trace methanol. The resulting material was dissolved in dichloromethane (0.5 mL).

(351) A crude solution of C2 was prepared separately, as follows: Bis(pentafluorophenyl) carbonate (5.8 g, 15 mmol) and triethylamine (41 mL, 290 mmol) were added to a stirring solution of C1 (3.75 g, 15.0 mmol) in tetrahydrofuran (30 mL). Sufficient tetrahydrofuran was added to bring the total volume to 98 mL, and the reaction mixture was stirred at room temperature for 1 hour. A portion of this crude C2 solution (1.0 mL, 0.15 mmol of C2 and 3 mmol of triethylamine) was added to the deprotected amine solution prepared above, and the reaction mixture was shaken at room temperature for 5 days. It was then partitioned between half-saturated aqueous sodium bicarbonate solution (1.5 mL) and ethyl acetate (2.4 mL) and subjected to vortexing. The organic layer was eluted through a solid phase extraction cartridge (6 mL) charged with sodium sulfate (1 g); this extraction procedure was repeated twice, and the combined eluents were concentrated in vacuo. This material was treated with a mixture of trifluoroacetic acid and 1,2-dichloroethane (1:1, 1 mL) and shaken at room temperature for 1 hour, whereupon it was concentrated in vacuo and purified using reversed phase HPLC (Column: Waters Sunfire C18, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 25% to 95% B). Yield: 4.8 mg, 11 mol, 7%. Analytical retention time: 2.64 minutes (Analytical HPLC conditionsColumn: Waters Atlantis dC18, 4.650 mm, 5 m; Mobile phase A: 0.05% trifluoroacetic acid in water (v/v); Mobile phase B: 0.05% trifluoroacetic acid in acetonitrile (v/v); Gradient: 5.0% to 95% B, linear over 4.0 minutes; Flow rate: 2 mL/minute). LCMS m/z 437.1 [M+H].sup.+.

Example 106

(2R)-1,1,1-Trifluoro-3-hydroxypropan-2-yl (3R)-3-{[(cyclopropylmethyl)sulfonyl](methyl)amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (106)

(352) ##STR00147## ##STR00148##

Step 1. Synthesis of tert-butyl (3R)-3-{[(prop-2-en-1-yloxy) carbonyl]amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C121)

(353) Prop-2-en-1-yl carbonochloridate (7.13 g, 59.2 mmol) was added drop-wise to a 0 C. solution of C94 (15.2 g, 39.4 mmol) in saturated aqueous sodium bicarbonate solution (160 mL) and tetrahydrofuran (40 mL). The reaction mixture was stirred at 10 C. for 14 hours, whereupon it was extracted with ethyl acetate (3100 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo to afford the product as a pale yellow gum (13.6 g). This material was used directly in the following step. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.98-5.85 (m, 1H), 5.31 (apparent br dd, J=17.2, 1.4 Hz, 1H), 5.23 (br d, J=10.3 Hz, 1H), 4.95-4.84 (m, 1H), 4.62-4.51 (m, 2H), 4.39-4.27 (m, 1H), 4.00 (dd, J=9.4, 5.6 Hz, 1H), 3.73-3.52 (m, 3H), 3.38-3.24 (m, 2H), 2.13 (dd, J=13.3, 7.8 Hz, 1H), 1.74-1.57 (m, 4H, assumed; partially obscured by water peak), 1.56-1.46 (m, 1H), 1.46 (s, 9H).

Step 2. Synthesis of tert-butyl (3R)-3-{methyl[(prop-2-en-1-yloxy)carbonyl]amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C122)

(354) Sodium hydride (60% dispersion in mineral oil; 2.36 g, 59.0 mmol) was added to a 0 C. solution of C121 (from the previous step; 13.4 g, 538.8 mmol) in tetrahydrofuran (200 mL), and the reaction mixture was stirred at 0 C. for 30 minutes. Iodomethane (16.8 g, 118 mmol) was added drop-wise, and stirring was continued for 16 hours at 0 C. to 5 C. Sodium hydride (60% dispersion in mineral oil; 2.36 g, 59.0 mmol) was again added, and the reaction mixture was stirred at 25 C. for 16 hours, whereupon it was poured into saturated aqueous ammonium chloride solution (200 mL) and extracted with ethyl acetate (3300 mL). The combined organic layers were washed with saturated aqueous sodium chloride solution (600 mL), dried over sodium sulfate, filtered, and concentrated under reduced pressure to afford the product as a brown gum (16 g). This was used in the following step without additional purification. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.99-5.89 (m, 1H), 5.34-5.27 (m, 1H), 5.24-5.19 (m, 1H), 5.09-4.85 (br m, 1H), 4.59 (ddd, J=5.5, 1.5, 1.4 Hz, 2H), 3.94 (dd, half of ABX pattern, J=9.7, 7.6 Hz, 1H), 3.76 (dd, half of ABX pattern, J=9.9, 5.4 Hz, 1H), 3.69-3.52 (m, 2H), 3.38-3.23 (m, 2H), 2.87 (s, 3H), 2.09 (dd, J=13.1, 9.0 Hz, 1H), 1.75-1.60 (m, 4H, assumed; partially obscured by water peak), 1.51-1.41 (m, 1H), 1.46 (s, 9H).

Step 3. Synthesis of prop-2-en-1-yl methyl[(3R)-1-oxa-8-azaspiro[4.5]dec-3-yl]carbamate, trifluoroacetate salt (C123)

(355) Trifluoroacetic acid (20 mL) was added to a solution of C122 (from the previous step; 16 g, 38.8 mmol) in dichloromethane (100 mL), and the reaction mixture was stirred at 15 C. for 2 hours. Removal of volatiles in vacuo afforded the product as a brown gum (20 g). This material was used directly in the following step. LCMS m/z 255.2 [M+H].sup.+.

Step 4. Synthesis of (2R)-1, 1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3R)-3-{methyl[(prop-2-en-1-yloxy)carbonyl]amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C124)

(356) Triethylamine (19.9 g, 197 mmol) was slowly added to a 0 C. solution of C123 (from the previous step; 20 g, 538.8 mmol) in acetonitrile (250 mL). The reaction mixture was stirred at 0 C. for 30 minutes, whereupon C2 [reaction solution in acetonitrile (80 mL) containing 40 mmol], was added, and stirring was continued at 13 C. for 18 hours. The reaction mixture was concentrated in vacuo, and the residue was purified via silica gel chromatography (Gradient: 9% to 50% ethyl acetate in petroleum ether) to provide the product as a pale yellow gum. Yield: 16.67 g, 31.4 mmol, 81% over 4 steps. LCMS m/z 553.1 [M+Na.sup.+]. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.24 (br d, J=8.8 Hz, 2H), 6.88 (br d, J=8.8 Hz, 2H), 6.01-5.89 (m, 1H), 5.53-5.43 (m, 1H), 5.35-5.27 (m, 1H), 5.26-5.20 (m, 1H), 5.08-4.86 (br m, 1H), 4.60 (ddd, J=5.5, 1.5, 1.2 Hz, 2H), 4.51 (AB quartet, J.sub.AB=11.5 Hz, .sub.AB=28.3 Hz, 2H), 3.94 (dd, J=9.8, 7.5 Hz, 1H), 3.81 (s, 3H), 3.80-3.64 (m, 5H), 3.43-3.25 (m, 2H), 2.88 (s, 3H), 2.13-2.00 (m, 1H), 1.80-1.60 (m, 4H), 1.47 (ddd, J=13.6, 10.8, 4.3 Hz, 1H).

Step 5. Synthesis of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3R)-3-(methylamino)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C125)

(357) Tetrakis(triphenylphosphine)palladium(0) (2.12 g, 1.83 mmol) was added to a 10 C. solution of C124 (6.50 g, 12.2 mmol) and 1,3-dimethylpyrimidine-2,4,6(1H,3H,5H)-trione (2.87 g, 18.4 mmol) in tetrahydrofuran (100 mL). After the reaction mixture had been stirred at 25 C. for 2 hours, solid sodium carbonate (65 mg, 0.61 mmol) was added, and stirring was continued at 10 C. for 20 minutes. The reaction mixture was filtered, and the filtrate was concentrated in vacuo. The residue was purified twice by silica gel chromatography (Gradient: 0% to 10% methanol in dichloromethane) to afford the product as a yellow gum. Yield: 3.8 g, 8.5 mmol, 70%. LCMS m/z 447.3 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.24 (br d, J=8.7 Hz, 2H), 6.88 (br d, J=8.7 Hz, 2H), 5.53-5.42 (m, 1H), 4.51 (AB quartet, J.sub.AB=11.6 Hz, .sub.AB=28.0 Hz, 2H), 3.96 (dd, J=9.2, 6.0 Hz, 1H), 3.81 (s, 3H), 3.8-3.64 (m, 5H), 3.43-3.28 (m, 3H), 2.43 (s, 3H), 2.08-1.97 (m, 1H), 1.85-1.46 (m, 5H, assumed; partially obscured by water peak).

Step 6. Synthesis of (2R)-1, 1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl (3R)-3-{[(cyclopropylmethyl)sulfonyl](methyl)amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (C126)

(358) To a 15 C. solution of C125 (400 mg, 0.896 mmol) in dichloromethane (5 mL) were added cyclopropylmethanesulfonyl chloride (208 mg, 1.35 mmol) and triethylamine (453 mg, 4.48 mmol). The reaction mixture was stirred at 15 C. for 16 hours, whereupon it was concentrated in vacuo and purified via chromatography on silica gel (Gradient: 0% to 50% ethyl acetate in petroleum ether). The product was obtained as a colorless gum. Yield: 430 mg, 0.762 mmol, 85%. LCMS m/z 587.1 [M+Na.sup.+].

Step 7. Synthesis of (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3R)-3-{[(cyclopropylmethyl)sulfonyl](methyl)amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate (106)

(359) Trifluoroacetic acid (3 mL) was added to a 0 C. solution of C126 (430 mg, 0.762 mmol) in dichloromethane (12 mL). The reaction mixture was stirred at 15 C. for 2 hours, whereupon the pH was adjusted to 6-7 via addition of sodium bicarbonate. The resulting mixture was extracted with dichloromethane (15 mL) and with ethyl acetate (215 mL). The combined organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. Silica gel chromatography (Gradient: 0% to 50% ethyl acetate in petroleum ether) was followed by reversed phase HPLC (Column: Agela Durashell C18, 5 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 30% to 50% B), affording the product as a colorless gum. Yield: 211 mg, 0.475 mmol, 62%. LCMS m/z 445.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 5.30-5.19 (m, 1H), 4.72-4.62 (m, 1H), 4.01-3.90 (m, 2H), 3.89-3.69 (m, 4H), 3.44-3.23 (m, 2H), 2.88-2.83 (m, 2H), 2.86 (s, 3H), 2.82-2.64 (br m, 1H), 2.13-2.01 (m, 1H), 1.81-1.65 (m, 4H), 1.55-1.39 (m, 1H), 1.13-1.01 (m, 1H), 0.76-0.62 (m, 2H), 0.42-0.29 (m, 2H).

(360) TABLE-US-00007 TABLE 6A Method of synthesis, structure, and physicochemical properties for Examples 107-150. Method of .sup.1H NMR (400 MHz, CDCl.sub.3) ; Mass Synthesis; spectrum, observed ion m/z [M + H].sup.+ or Example Non-commercial HPLC retention time; Mass spectrum m/z Number starting materials Structure [M + H].sup.+ (unless otherwise indicated) 107 Example 92.sup.35; C48, C2 7.83 (d, J = 7.5 Hz, 2H), 7.57 (br dd, half of ABX pattern, J = 7.5, 7.0 Hz, 1H), 7.51 (br dd, half of ABX pattern, J = 7.5, 7.0 Hz, 2H), 5.31-5.19 (m, 1H), 4.16-3.95 (m, 3H), 3.92- 3.68 (m, 5H), 3.50-3.30 (m, 2H), 2.52-2.35 (m, 1H), 2.29-2.15 (m, 1H), 1.99-1.82 (m, 2H), 1.79-1.47 (m, 3H, assumed; partially obscured by water peak), 1.26-1.18 (m, 6H); 495.1 108 Example 11.sup.36,37; C73 0 .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.72-7.63 (m, 3H), 7.61-7.55 (m, 1H), 5.24-5.14 (m, 1H), 4.68-4.59 (m, 1H), 3.77-3.59 (m, 4H), 3.57-3.44 (m, 3H), 3.3-3.12 (m, 2H), 2.70 (s, 3H), 1.96-1.85 (m, 1H), 1.65-1.49 (m, 3H), 1.49-1.35 (m, 2H); 485.3 109 Example 11.sup.36,37; C73 .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.73-7.62 (m, 3H), 7.62-7.54 (m, 1H), 5.23-5.14 (m, 1H), 4.69-4.58 (m. 1H), 3.78-3.58 (m, 4H), 3.58-3.44 (m, 3H), 3.3-3.11 (m, 2H), 2.70 (s, 3H), 1.91 (dd, J = 13.4, 9.1 Hz, 1H), 1.67-1.49 (m, 3H), 1.48-1.31 (m, 2H); 485.3 110 Example 109.sup.38; C73 .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.91-7.84 (m, 2H), 7.46 (br dd, J = 8.8, 8.8 Hz, 2H), 5.24-5.14 (m, 1H), 4.64-4.55 (m, 1H), 3.78- 3.68 (m, 3H), 3.68-3.58 (m, 1H), 3.57-3.43 (m, 3H), 3.3-3.12 (m, 2H), 2.67 (s, 3H), 1.89 (br dd, J = 13, 9.5 Hz, 1H), 1.65-1.49 (m, 3H), 1.49-1.34 (m, 2H); 485.3 111 Example 109.sup.38; C73 .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.91-7.85 (m, 2H), 7.46 (br dd, J = 8.8, 8.8 Hz, 2H), 5.24-5.14 (m, 1H), 4.64-4.55 (m, 1H), 3.78- 3.68 (m, 3H), 3.68-3.59 (m, 1H), 3.57-3.44 (m, 3H), 3.3-3.13 (m, 2H), 2.67 (s, 3H), 1.89 (br dd, J = 13, 9.5 Hz, 1H), 1.65-1.48 (m, 3H), 1.48-1.34 (m, 2H); 485.3 112 Examples 93 and 94.sup.39; C73 Mixture of 2 diastereomers; characteristic peaks: 5.30-5.20 (m, 1H), 4.76-4.66 (m, 1H), 4.05-3.92 (m, 2H), 3.92-3.70 (m, 4H), 3.48-3.23 (m, 3H), 2.87 (s, 3H), 2.35-2.22 (m, 1H), 2.13-2.02 (m, 1H), 2.02-1.91 (m, 3H), 1.88-1.59 (m, 7H); 459.3 113 Examples 93 and 94.sup.39; C73 Mixture of 2 diastereomers; 5.32-5.19 (m, 1H), 4.72-4.61 (m, 1H), 4.05-3.96 (m, 1H), 3.96-3.68 (m, 6H), 3.44-3.19 (m, 2H), 2.83 (s, 3H), 2.60-2.46 (m, 2H), 2.46-2.19 (m, 3H), 2.10-1.96 (m, 3H), 1.81-1.67 (m, 4H), 1.55-1.43 (m, 1H); 445.2 114 Example 11.sup.40,39; C73 Mixture of 2 diastereomers; 9.02 (d, J = 2.0 Hz, 1H), 8.85 (dd, J = 4.8, 1.5 Hz, 1H), 8.09 (ddd, J = 8.0, 2.1, 1.8 Hz, 1H), 7.51 (dd, J = 8.0, 5.0 Hz, 1H), 5.29-5.18 (m, 1H), 4.80-4.69 (m, 1H), 4.04-3.94 (m, 1H), 3.91- 3.69 (m, 4H), 3.65 (dd, J = 10. 4.5 Hz, 1H), 3.38-3.13 (m, 2H), 2.81 (s, 3H), 2.37-2.22 (m, 1H), 1.98-1.86 (m, 1H), 1.74-1.6 (m, 3H), 1.54-1.39 (m, 2H); 468.0 115 Example 114.sup.41; C73 Mixture of 2 diastereomers; 9.42 (s, 1H), 9.10 (s, 2H), 5.29-5.19 (m, 1H), 4.81-4.71 (m, 1H), 4.04-3.95 (m, 1H), 3.95-3.74 (m, 4H), 3.70 (dd, J = 10.4, 4.6 Hz, 1H), 3.38- 3.14 (m, 2H), 2.85 (s, 3H), 2.45-2.22 (br m, 1H), 2.05-1.91 (m, 1H), 1.76-1.6 (m, 3H, assumed; partially obscured by water peak), 1.52 (dd, J = 13.6, 6.5 Hz, 1H), 1.5- 1.40 (m, 1H); 469.0 116 Example 92.sup.42,43; C99, C2 5.31-5.20 (m, 1H), 4.70-4.61 (m, 1H), 4.14- 4.05 (m, 2H), 4.05-3.92 (m, 2H), 3.92-3.70 (m, 4H), 3.43-3.20 (m, 4H), 3.16-3.05 (m, 1H), 2.89 (s, 3H), 2.44-2.23 (m, 1H), 2.10 (dd, J = 13.3, 9.0 Hz, 1H), 1.98-1.67 (m, 8H), 1.6-1.46 (m, 1H); 475.1 117 Example 92.sup.42,43; C99, C2 5.32-5.20 (m, 1H), 4.70-4.60 (m, 1H), 4.14- 4.06 (m, 2H), 4.05-3.92 (m, 2H), 3.92-3.73 (m, 4H), 3.46-3.26 (m, 4H), 3.16-3.06 (m, 1H), 2.89 (s, 3H), 2.41-2.24 (m, 1H), 2.15- 2.04 (m, 1H), 1.98-1.68 (m, 8H), 1.6-1.42 (m, 1H); 475.1 118 Example 102.sup.44; C99, C1 0 Mixture of 2 diastereomers; 3.35 minutes.sup.13; 458.2 119 Example 102.sup.45; C1 Mixture of 2 diastereomers; 3.25 minutes.sup.13; 408.2 120 Example 97; C107 Mixture of 2 diastereomers; 2.45 minutes.sup.13; 393.3 121 Example 97; C107 Mixture of 2 diastereomers; 2.18 minutes.sup.13; 405.3 122 Example 97; C107 Mixture of 2 diastereomers; 1.40 minutes.sup.13; 375.1 123 Example 97; C107 Mixture of 2 diastereomers; 2.36 minutes.sup.13; 389.3 124 Example 98; C2 5.33-5.20 (m, 1H), 4.02-3.91 (m, 1H), 3.89- 3.77 (m, 1H), 3.76-3.07 (m, 9H), 2.81-2.66 (m, 1H), 1.90-1.66 (m, 8H), 1.64-1.46 (m, 6H); 392.9 125 Example 105.sup.46; C2 2.35 minutes.sup.13; 408.2 126 Example 105.sup.46; C2 2.38 minutes.sup.13; 401.3 127 Example 98; C2 Characteristic peaks: 5.33-5.20 (m, 1H), 4.05-3.96 (m, 1H), 3.92-3.82 (m, 1H), 3.78- 3.24 (m, 8H), 2.47-2.33 (m, 1H), 2.29-2.15 (m, 2H), 1.99-1.65 (m, 9H); 443.0 128 Example 105.sup.46; C2 0 1.97 minutes.sup.13; 402.3 129 Example 92.sup.47,48; C2 .sup.1H NMR (400 MHz, CD.sub.3OD) 8.11 (ddd, J = 5.0, 2.0, 0.8 Hz, 1H), 7.67 (ddd, J = 8.4, 7.1, 2.0 Hz, 1H), 6.94 (ddd, J = 7.2, 5.1, 0.9 Hz, 1H), 6.79 (ddd, J = 8.3, 0.9, 0.8 Hz, 1H), 5.55-5.50 (m, 1H), 5.33-5.24 (m, 1H), 4.18 (dd, J = 10.5, 4.6 Hz, 1H), 4.01-3.96 (m, 1H), 3.90-3.84 (m, 1H), 3.84-3.69 (m, 3H), 3.50-3.3 (m, 2H), 2.20 (dd, half of ABX pattern, J = 13.9, 6.8 Hz, 1H), 2.07 (ddd, half of ABXY pattern, J = 14.0, 2.0, 1.1 Hz, 1H), 1.98-1.88 (m, 1H), 1.78-1.62 (m, 3H); 390.9 130 Example 92.sup.47,48; C2 .sup.1H NMR (400 MHz, CD.sub.3OD) 8.11 (ddd, J = 5.0, 2.0, 0.8 Hz, 1H), 7.67 (ddd, J = 8.5, 7.0, 2.0 Hz, 1H), 6.94 (ddd, J = 7.0, 5.0, 1.0 Hz, 1H), 6.78 (br d, J = 8.5 Hz, 1H), 5.55- 5.50 (m, 1H), 5.33-5.24 (m, 1H), 4.18 (dd, J = 10.4, 4.6 Hz, 1H), 3.99 (ddd, J = 10.4, 2.0, 1.1 Hz, 1H), 3.87 (br dd, half of ABX pattern, J = 12.3, 4.0 Hz, 1H), 3.84-3.70 (m, 3H), 3.50-3.33 (m, 2H), 2.20 (dd, half of ABX pattern, J = 13.9, 6.7 Hz, 1H), 2.07 (br d, J = 14.0 Hz, 1H), 1.98-1.85 (m, 1H), 1.81- 1.58 (m, 3H); 390.9 131 Example 101; C105 Mixture of 2 diastereomers; 7.85 (br d, J = 7.8 Hz, 1H), 7.52-7.46 (m, 1H), 7.44- 7.38 (m, 1H), 7.29-7.23 (m, 1H, assumed; partially obscured by solvent peak), 5.30- 5.19 (m, 1H), 4.59-4.45 (m, 1H), 4.11-3.95 (m, 2H), 3.92-3.68 (m, 6H), 3.46-3.20 (m, 2H), 3.12-3.03 (m, 2H), 2.41-2.24 (m, 1H), 2.21-2.11 (m, 1H), 1.88-1.79 (m, 1H), 1.79- 1.64 (m, 3H), 1.6-1.43 (m, 1H, assumed; partially obscured by water peak); 479.2 132 Example 102.sup.49; C1 Mixture of 2 diastereomers; 2.92 minutes.sup.13; 404.2 133 C2.sup.50,51 5.31-5.21 (m, 1H), 3.96 (dd, half of ABX pattern, J = 12.4, 3.4 Hz, 1H), 3.83 (dd, half of ABX pattern, J = 12.3, 6.8 Hz, 1H), 3.66- 3.29 (m, 10H), 3.29-3.22 (br s, 2H), 1.87- 1.77 (m, 4H), 1.74 (dd, J = 7.3, 7.0 Hz, 2H), 1.62-1.47 (m, 4H); 394.1 134 Example 6.sup.50 8.00 (d, J = 3.0 Hz, 1H), 7.66 (d, J = 3.0 Hz, 1H), 5.29-5.19 (m, 1H), 4.03-3.94 (m, 1H), 3.90-3.80 (m, 1H), 3.67-3.47 (m, 4H), 3.45- 3.18 (m, 4H), 2.58-2.43 (br s, 1H), 1.79 (dd, J = 7.0, 7.0 Hz, 2H), 1.51-1.37 (m, 4H); 443.8 135 Example 6.sup.50 9.09-9.05 (br s, 1H), 8.85 (d, J = 4.5 Hz, 1H), 8.13 (br d, J = 8.0 Hz, 1H), 7.52 (dd, J = 7.9, 4.9 Hz, 1H), 5.29-5.20 (m, 1H), 3.99 (br dd, half of ABX pattern, J = 12.5, 3 Hz, 1H), 3.85 (br dd, half of ABX pattern, J = 12, 7 Hz, 1H), 3.59-3.25 (m, 6H), 3.21-3.11 (m, 2H), 2.47-2.30 (br s, 1H), 1.74 (dd, J = 7.0, 7.0 Hz, 2H), 1.47-1.39 (m, 4H); 437.9 136 Example 106; C125 7.98 (d, J = 3.0 Hz, 1H), 7.65 (d, J = 3.0 Hz, 1H), 5.30-5.19 (m, 1H), 4.94-4.84 (m, 1H), 4.04-3.95 (m, 1H), 3.92 (dd, half of ABX pattern, J = 10.4, 7.4 Hz, 1H), 3.91-3.71 (m, 4H), 3.42-3.21 (m, 2H), 2.95 (s, 3H), 2.10- 1.97 (m, 1H), 1.7-1.37 (m, 5H, assumed; partially obscured by water peak); 474.0 137 Example 106; C125 5.32-5.20 (m, 1H), 4.75-4.65 (m, 1H), 4.05- 3.93 (m, 2H), 3.93-3.7 (m, 4H), 3.74 (q, J.sub.HF = 9.3 Hz, 2H), 3.45-3.23 (m, 2H), 2.93 (s, 3H), 2.19-2.07 (m, 1H), 1.84-1.67 (m, 4H), 1.57-1.41 (m, 1H); 473.2 138 Example 106; C125 0 7.76 (s, 1H), 7.70 (s, 1H), 5.30-5.18 (m, 1H), 4.66-4.54 (m, 1H), 4.03-3.93 (m, 1H), 3.96 (s, 3H), 3.88-3.71 (m, 3H), 3.87 (dd, half of ABX pattern, J = 10.2, 7.4 Hz, 1H), 3.67 (br dd, half of ABX pattern, J = 10.2, 5.1 Hz, 1H), 3.40-3.19 (m, 2H), 2.74 (s, 3H), 2.69-2.52 (m, 1H), 2.01-1.88 (m, 1H), 1.82-1.63 (m, 3H, assumed; partially obscured by water peak), 1.58 (dd, J = 13.3, 7.0 Hz, 1H), 1.51-1.36 (m, 1H); 471.2 139 Example 106; C125 5.31-5.19 (m, 1H), 4.70-4.59 (m, 1H), 4.03- 3.90 (m, 2H), 3.90-3.70 (m, 4H), 3.45-3.23 (m, 2H), 2.82 (s, 3H), 2.75 (br d, J = 6.5 Hz, 2H), 2.73-2.64 (m, 1H), 2.30-2.16 (m, 1H), 2.12-2.00 (m, 1H), 1.80-1.66 (m, 4H), 1.55- 1.40 (m, 1H), 1.09 (br d, J = 6.8 Hz, 6H); 447.3 140 Example 106; C125 5.31-5.19 (m, 1H), 4.67-4.56 (m, 1H), 4.03- 3.90 (m, 2H), 3.90-3.70 (m, 4H), 3.45-3.24 (m, 2H), 2.99 (br d, J = 7.5 Hz, 2H), 2.84- 2.70 (m, 1H), 2.81 (s, 3H), 2.69-2.58 (m, 1H), 2.25-2.14 (m, 2H), 2.12-2.01 (m, 1H), 2.01-1.91 (m, 1H), 1.91-1.79 (m, 3H), 1.79- 1.66 (m, 4H), 1.55-1.40 (m, 1H); 459.2 141 C125.sup.52 2.82 minutes.sup.26; 449 142 C125.sup.52 2.47 minutes.sup.53; 409 143 C125.sup.52 2.86 minutes.sup.26; 423 144 C125.sup.52 2.80 minutes.sup.26; 411 145 C125.sup.52 2.44 minutes.sup.53; 409 146 C125.sup.52 2.26 minutes.sup.53; 432 147 Example 97; C107 Mixture of 2 diastereomers; 2.77 minutes.sup.13; 399.3 148 Example 33.sup.54,55; C79 0 .sup.1H NMR (400 MHz, D.sub.2O), characteristic peaks: 7.84 (br d, J = 7.6 Hz, 2H), 7.76- 7.69 (m, 1H), 7.63 (br dd, half of ABX pattern, J = 7.8, 7.6 Hz, 2H), 5.44-5.34 (m, 1H), 4.10-4.01 (m, 1H), 4.00-3.90 (m, 1H), 3.90-3.81 (m, 1H), 3.70 (t, J = 6.0 Hz, 2H), 3.65-3.22 (m, 5H), 2.98 (dd, J = 7.6, 7.4 Hz, 4H), 2.76 (s, 3H), 1.95 (dd, J = 13.7, 9.2 Hz, 1H), 1.91-1.81 (m, 4H), 1.74-1.62 (m, 7H), 1.54-1.33 (m, 6H); 547.3 149 Example 33.sup.56,57; Example 91 .sup.1H NMR (400 MHz, D.sub.2O) 7.83-7.78 (m, 2H), 7.77-7.72 (m, 1H), 7.65 (br dd, J = 7.8, 7.4 Hz, 2H), 5.47-5.37 (m, 1H), 4.13-4.05 (m, 1H), 4.02-3.93 (m, 1H), 3.93-3.79 (m, 1H), 3.83 (br dd, J = 5.1, 4.9 Hz, 2H), 3.78- 3.63 (m, 1H), 3.71 (t, J = 6.1 Hz, 2H), 3.36- 3.13 (m, 2H), 3.11-3.0 (m, 2H), 2.99 (dd, J = 7.6, 7.4 Hz, 4H), 2.95-2.86 (m, 2H), 2.00-1.82 (m, 6H), 1.74-1.64 (m, 6H), 1.53- 1.34 (m, 4H); 533.1 150 Example 33.sup.58,59; C125 .sup.1H NMR (400 MHz, D.sub.2O), characteristic peaks: 7.92-7.85 (m, 2H), 7.35 (br dd, J = 8.9, 8.8 Hz, 2H), 5.44-5.35 (m, 1H), 4.78-4.66 (m, 1H, assumed; partially obscured by solvent peak), 4.10-4.02 (m, 1H), 3.99-3.91 (m, 1H), 3.87 (dd, J = 10.0, 7.9 Hz, 1H), 3.68 (t, J = 6.1 Hz, 2H), 3.67- 3.60 (m, 2H), 2.98 (dd, J = 7.7, 7.5 Hz, 4H), 2.77 (s, 3H), 1.97 (dd, J = 13.5, 9.3 Hz, 1H), 1.91-1.79 (m, 5H), 1.73-1.63 (m, 5H), 1.55- 1.33 (m, 7H); 565.3 35. Intermediate tert-butyl (3R)-3-[(phenylsulfonyl)(propan-2-yl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate was synthesized via a Mitsunobu reaction between C48 and 2-propanol.36. Prior to the final deprotection, intermediate (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-{[(3-fluorophenyl)sulfonyl]amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate was deprotonated with potassium tert-butoxide and methylated with dimethyl sulfate to afford (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-{[(3-fluorophenyl)sulfonyl](methyl)amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate. 37. Examples 108 and 109 were synthesized as a mixture and separated into the component diastereomers using supercritical fluid chromatography (Column: Phenomenex Lux Amylose-2,5 m; Mobile phase: 87.5:12.5 carbon dioxide/2-propanol). Example 108 was the first-eluting diastereomer, and Example 109 was the second-eluting diastereomer. 38. Examples 110 and 111 were synthesized as a mixture and separated into the component diastereomers using supercritical fluid chromatography (Column: Phenomenex Lux Amylose-2,5 m; Mobile phase: 85:15 carbon dioxide/2-propanol). Example 110 was the first-eluting diastereomer, and Example 111 was the second-eluting diastereomer. 39. In this case, the 2 diastereomers of the product were not separated. 40. Prior to the final deprotection, intermediate (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-[(pyridin-3-ylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate was deprotonated with sodium bis(trimethylsilyl)amide and methylated with iodomethane, affording (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-[methyl(pyridin-3-ylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate. 41. In this case, the sulfonylation of C73 was effected using pyridine in tetrahydrofuran, rather than aqueous sodium bicarbonate in dichloromethane. 42. The requisite tert-butyl 3-[methyl(tetrahydro-2H-pyran-4-ylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate was synthesized via potassium carbonate-mediated reaction of C99 with tetrahydro-2H-pyran-4-sulfonamide. The resulting tert-butyl 3-[(tetrahydro-2H-pyran-4-ylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate was then methylated using sodium hydride and iodomethane. 43. Prior to the final deprotection, intermediate (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 3-[methyl(tetrahydro-2H-pyran-4-ylsulfonyl)amino]-1-oxa-8-azaspiro[4.5]decane-8-carboxylate was separated into its component diastereomers via supercritical fluid chromatography (Column: Chiral Technologies Chiralcel OD, 5 m; Mobile phase A: carbon dioxide; Mobile phase B: 0.1% ammonium hydroxide in 2-propanol; Gradient: 30% to 35% B). The first-eluting diastereomer was assigned as DIAST-1, and the second eluting diastereomer as DIAST-2. 44. Reaction of C99 with [3-(trifluoromethoxy)phenyl]boronic acid was carried out using the method described for synthesis of the mixture of C100 and C101 from C99 in Example 95. The product was deprotected with hydrogen chloride in 1,4-dioxane and dichloromethane to afford the requisite 3-[3-(trifluoromethoxy)phenyl]-1-oxa-8-azaspiro[4.5]decane, hydrochloride salt. 45. Reaction of tert-butyl 3-oxo-1-oxa-8-azaspiro[4.5]decane-8-carboxylate with 3-chlorophenylmagnesium bromide provided tert-butyl 3-(3-chlorophenyl)-3-hydroxy-1-oxa-8-azaspiro[4.5]decane-8-carboxylate. This was subjected to triethylsilane, boron trifluoride diethyl etherate and trifluoroacetic acid, providing partial deoxygenation, followed by hydrogenation in methanol and acetic acid, to afford 3-(3-chlorophenyl)-1-oxa-8-azaspiro[4.5]decane. 46. In this case, the first step was an amide formation, rather than a sulfonamide formation. tert-Butyl 2,8-diazaspiro[4.5]decane-8-carboxylate was reacted with the appropriate carboxylic acid using 2-[2-oxo-1(2H)-pyridyl]-1,1,3,3-tetramethyluronium tetrafluoroborate (TPTU) and N,N-diisopropylethylamine in N,N-dimethylformamide. 47. tert-Butyl 3-hydroxy-1-oxa-8-azaspiro[4.5]decane-8-carboxylate was deprotonated with potassium tert-butoxide and reacted with 2-chloropyridine to afford the requisite tert-butyl 3-(pyridin-2-yloxy)-1-oxa-8-azaspiro[4.5]decane-8-carboxylate. 48. The mixture of Examples 129 and 130 was separated into its component diastereomers using reversed phase HPLC (Column: Phenomenex Luna C18, 5 m; Mobile phase A: water containing 0.225% formic acid; Mobile phase B: acetonitrile; Gradient: 25% to 45% B). The first-eluting diastereomer was Example 129, and the second-eluting diastereomer was Example 130. 49. Reaction of tert-butyl 3-hydroxy-1-oxa-8-azaspiro[4.5]decane-8-carboxylate with sodium hydride and benzyl bromide afforded tert-butyl 3-(benzyloxy)-1-oxa-8-azaspiro [4.5]decane-8-carboxylate, which was deprotected with hydrochloric acid to provide the requisite 3-(benzyloxy)-1-oxa-8-azaspiro[4.5]decane. 50. tert-Butyl 2,8-diazaspiro[4.5]decane-8-carboxylate was converted to (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 2,8-diazaspiro [4.5]decane-8-carboxylate using the method described for synthesis of C73 from tert-butyl 3-amino-1-oxa-8-azaspiro[4.5]decane-8-carboxylate in Example 27. In this case, the palladium catalyst employed for the final step was tetrakis(triphenylphosphine)palladium(0). 51. Reaction of (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 2,8-diazaspiro[4.5]decane-8-carboxylate (see footnote 50) with 4-nitrophenyl pyrrolidine-1-carboxylate (see E. Bridgeman and N. C. O. Tomkinson, Synlett 2006, 243-246) in the presence of N,N-diisopropylethylamine provided (2R)-1,1,1-trifluoro-3-[(4-methoxybenzyl)oxy]propan-2-yl 2-(pyrrolidin-1-ylcarbonyl)-2,8-diazaspiro[4.5]decane-8-carboxylate, which was deprotected with trifluoroacetic acid to afford Example 133. 52. Compound C125 was reacted with the appropriate carboxylic acid using 2,4,6-tripropyl-1,3,5,2,4,6-trioxatriphosphinane 2,4,6-trioxide and N,N-diisopropylethylamine in 1,4-dioxane. The resulting product was deprotected with trifluoroacetic acid to afford the Example. 53. Conditions for analytical HPLC. Column: Waters XBridge C18, 2.1 50 mm, 5 m; Mobile phase A: 0.05% ammonium hydroxide in water; Mobile phase B: acetonitrile; Gradient: 5% B for 0.5 minutes; 5% to 100% B over 2.9 minutes; 100% B for 0.8 minutes; Flow rate: 0.8 mL/minute. 54. In this case, C79 was synthesized from C50, via deprotection with p-toluenesulfonic acid and conversion of the resulting amine to C79 using the method described for synthesis of C84 from C85 in Alternate Synthesis of Example 32.55. In this case, the final product did not precipitate out of the reaction mixture. The reaction mixture was therefore concentrated in vacuo; the residue was dissolved in hot methanol, filtered, concentrated under reduced pressure, and crystallized from methanol/tert-butyl methyl ether to afford Example 148.56. An aqueous solution of Example 91 was acidified with concentrated hydrochloric acid at 0 C. After 30 minutes at room temperature, the reaction mixture was extracted three times with ethyl acetate, and the combined organic layers were washed with saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered, and concentrated in vacuo to afford the requisite (neutral) phosphate of Example 91.57. In this case, the final product did not precipitate out of the reaction mixture. The reaction mixture was therefore concentrated in vacuo; the residue was dissolved in hot methanol, cooled to 0 C. and treated with tert-butyl methyl ether. Filtration afforded Example 149.58. Using the method described in Example 11, C125 was converted to (2R)-1,1,1-trifluoro-3-hydroxypropan-2-yl (3R)-3-{[(4-fluorophenyl)sulfonyl](methyl)amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate. Phosphate formation, using the chemistry employed for conversion of 15 to C79 in Example 30, then provided the requisite (2R)-1,1,1-trifluoro-3-(phosphonooxy)propan-2-yl (3R)-3-{[(4-fluorophenyl)sulfonyl](methyl)amino}-1-oxa-8-azaspiro[4.5]decane-8-carboxylate. 59. In this case, after the reaction mixture had been heated at 40 C., it was slowly treated with tert-butyl methyl ether (3 mL). After the suspension had cooled to room temperature and then to 0 C., it was filtered to afford Example 150.

Example AA: MAGL and FAAH Enzymatic Assays

(361) Assessment of MAGL inhibition utilizes human recombinant Monoacylglycerol Lipase and the fluorogenic substrate 7-hydroxycoumarinyl arachidonate (7-HCA, Biomol ST-502). 400 nL of a test compound at decreasing concentration (ranging from 150 M down to 1.5 nM) was spotted into a 384-well back plate (PerkinElmer, 6007279) using a Labcyte Echo, followed by addition of 10 L of MAGL enzyme in assay buffer (50 mM HEPES, pH 7.4, 100 mM NaCl, 5 mM MgCl.sub.2, 0.1% Triton X-100 and 25% glycerin). An equal volume of 7-HCA in assay buffer with 10% DMSO was added either immediately (T=0 min) or after a 30 minute incubation (T=30 min) to initiate the reaction. The final concentration of MAGL enzyme was 88 M and 7-HCA substrate was 5 M. After these dilutions, the final concentration of the test compound ranged from 3 M to 0.03 nM. The reaction was allowed to progress for 60 minutes, after which the plate was read at an Ex/Em of 340/465. Percent inhibitions were calculated based on control wells containing no compound (0% inhibition) and a control compound (e.g., a MAGL inhibitor whose activity is known or was previously reported in the literature, such as one with about 100% inhibition). IC.sub.50 values were generated based on a four parameter fit model using ABASE software from IDBS. See e.g., Wang, Y. et al., A Fluorescence-Based Assay for Monoacylglycerol Lipase Compatible with Inhibitor Screening, Assay and Drug Development Technologies, 2008, Vol. 6 (3) pp 387-393 (reporting an assay for measuring MAGL activity).

(362) To measure MAGL inactivation, the same protocol for the (T=0 min) MAGL inhibition IC.sub.50 assay was performed with data collected every minute to acquire enzyme progress curves at decreasing concentrations of compound. K.sub.obs values were calculated from this data and k.sub.inact/K.sub.I ratios were determined from a plot of K.sub.obs values vs. compound concentrations.

(363) Assessment of FAAH inhibition utilizes human recombinant FAAH and the fluorescent substrate, Arachidonoyl-AMC. 400 nL of a test compound at decreasing concentrations was spotted into a 384-well back plate (PerkinElmer, 6007279) using a Labcyte Echo, followed by addition of 10 l of FAAH enzyme (Cayman 10010183) in assay buffer (50 mM Tris, pH 9.0, 1 mM EDTA). After a 30 minute incubation at room temperature, 10 L of Arachidonyl-AMCA was added in assay buffer with 16% DMSO. Final concentration of FAAH enzyme was 0.0125 Units and AAMCA substrate was used at the K.sub.m of 5 M. After these dilutions, the final concentration of the test compound ranged from 3 M to 0.03 nM. The reaction was allowed to progress for 60 minutes, after which the plate was read on a Molecular Devices FlexStation reader at an Ex/Em of 355/460. Percent inhibitions were calculated based on controls wells containing either no compound (0% inhibition) or a control compound (e.g., an FAAH inhibitor whose activity is known or was previously reported in the literature, such as one with about 100% inhibition). IC.sub.50 values were generated based on a four parameter fit model using ABASE software from IDBS.

(364) TABLE-US-00008 TABLE AA-1 Biological Data (MAGL IC.sub.50, FAAH IC.sub.50, and MAGL k.sub.inact/K.sub.I) for Examples 1-150. Ex- ample MAGL MAGL FAAH MAGL Num- (T = 0 min) (T = 30 min) (T = 30 min) k.sub.inact/K.sub.I ber Compound Name IC.sub.50 (M).sup.a IC.sub.50 (M).sup.a IC.sub.50 (M).sup.a (1/s per M).sup.a 1 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.085 0.014 N.D..sup.b 7806 yl (1a,5,6)-6-[1-(5-methoxypyridin-2- yl)-1H-pyrazol-3-yl]-3- azabicyclo[3.1.0]hexane-3-carboxylate 2 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.056 0.008 1.14.sup.d 6109 yl 4-[1-(4-fluorophenyl)-1H-pyrazol-3- yl]piperidine-1-carboxylate 3 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.035 0.003 2.48 20005 yl (1,5,6)-6-[1-(4-fluorophenyl)-1H- pyrazol-3-yl]-3- azabicyclo[3.1.0]hexane-3-carboxylate 4 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.166.sup.d 0.019.sup.d N.D. 5489 yl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1- oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate [from C25, DIAST-1] 5 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 1.70.sup.d 0.161.sup.d N.D. N.D. yl 4-(tetrahydro-2H-pyran-3-ylmethyl)-1- oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate [from C26, DIAST-2] 6 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.083.sup.c 0.007.sup.c >30.0.sup.d 13406 yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate 7 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.029.sup.c 0.003.sup.c >24.1 29124 yl 4-(phenylsulfonyl)-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 8 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.057 0.005 >30.0.sup.d 6754 yl (3S)-3-[(phenylsulfonyl)amino]-1-oxa- 8-azaspiro[4.5]decane-8-carboxylate 9 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.040 0.004 >30.0.sup.d 8588 yl (3R)-3-[(phenylsulfonyl)amino]-1-oxa- 8-azaspiro[4.5]decane-8-carboxylate 10 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.077.sup.c 0.007.sup.c >30.0.sup.d 5205 yl 4-[(5-cyclopropylpyridin-2- yl)oxy]piperidine-1-carboxylate 11 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.014 0.001 >30.0.sup.d 147964 yl 4-[(3-fluorophenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate 12 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.188 0.017 >30.0.sup.d 1801 yl 2-[(4-fluorophenyl)sulfonyl]-2,9- diazaspiro[5.5]undecane-9-carboxylate 13 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.368 0.035 N.D. N.D. yl (3aR,6aS)-5-[(3,4- difluorophenyl)sulfonyl]hexahydropyrrolo [3,4-c]pyrrole-2(1H)-carboxylate 14 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.485 0.045 N.D. N.D. yl 4-(5-fluoropyridin-2-yl)-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 15 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.017 0.002 N.D. 44421 yl (3R)-3- [methyl(phenylsulfonyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 16 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 1.84 0.161 N.D. N.D. yl 4-hydroxy-4- {[(phenylsulfonyl)amino]methyl}piperidine- 1-carboxylate 17 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.951 0.110 N.D. N.D. yl 4-(4-fluorobenzyl)-3-oxo-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 18 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.524 0.049 N.D. N.D. yl 2-ethyl-4-[(4-fluorophenyl)sulfonyl]-1- oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate 19 1,1,1,3,3-pentafluoro-4-hydroxybutan- 0.095 0.008 >30.0.sup.d 2397 2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate 20 1,1,1,3,3-pentafluoro-4-hydroxybutan- 0.061 0.006 >30.0.sup.d 4611 2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate, ENT-1 21 1,1,1,3,3-pentafluoro-4-hydroxybutan- 0.599 0.053 N.D. N.D. 2-yl 4-[(4-fluorophenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate, ENT-2 22 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 2.638 0.192 N.D. N.D. yl 4-(morpholin-4-ylsulfonyl)-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 23 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- >1.52 0.078 N.D. N.D. yl 3-(4-fluorobenzyl)-3,8- diazabicyclo[3.2.1]octane-8-carboxylate 24 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 1.90.sup.d 0.195.sup.d N.D. N.D. yl 4-hydroxy-4- {[methyl(phenylsulfonyl)amino]methyl} piperidine-1-carboxylate 25 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.794.sup.d 0.071.sup.d N.D. N.D. yl 4-(4-fluorobenzyl)piperazine-1- carboxylate 26 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.031 0.002 7.73.sup.d 16949 yl 4-(isoquinolin-1-yloxy)piperidine-1- carboxylate, trifluoroacetic acid salt 27 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.484 0.043 N.D. N.D. yl 3-(pyridin-2-ylamino)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 28 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.696 0.085 N.D. N.D. yl 4-(4-fluorobenzyl)-1-oxa-3-thia-4,9- diazaspiro[5.5]undecane-9-carboxylate 3,3-dioxide 29 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.109.sup.c 0.024.sup.c N.D. 85270 yl 4-[(4-fluorophenyl)sulfonyl]-3- hydroxy-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 30 (2R)-3,3,3-trifluoro-2-[({(3R)-3- >3.00.sup.d,e 0.549.sup.e N.D. N.D. [methyl(phenylsulfonyl)amino]-1-oxa-8- azaspiro[4.5]dec-8- yl}carbonyl)oxy]propyl phosphate, disodium salt 31 (2R)-3,3,3-trifluoro-2-[({(3R)-3- >3.00.sup.d >3.00.sup.d N.D. N.D. [(phenylsulfonyl)amino]-1-oxa-8- azaspiro[4.5]dec-8- yl}carbonyl)oxy]propyl phosphate disodium salt 32 (2R)-3,3,3-trifluoro-2-[({4-[(4- >3.00.sup.d,e >3.00.sup.d,e N.D. N.D. fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undec-9- yl}carbonyl)oxy]propyl phosphate, disodium salt 33 (2R)-3,3,3-trifluoro-2-[({4-[(4- >3.00.sup.d,e >3.00.sup.d,e N.D. N.D. fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undec-9- yl}carbonyl)oxy]propyl phosphate, (bis)- L-lysine salt 34 (2R)-3,3,3-trifluoro-2-[({4-[(3- N.D. N.D. N.D. N.D. fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undec-9- yl}carbonyl)oxy]propyl phosphate, disodium salt 35 1,1,1-trifluoro-3-hydroxypropan-2-yl 4- 0.339 0.032.sup.d N.D. N.D. [2-(morpholin-4-yl)pyrimidin-4- yl]piperidine-1-carboxylate 36 rel-(2S,3R)-1,1,1,4,4,4-hexafluoro-3- 0.132 0.012 13.2 3736 hydroxybutan-2-yl (1,5,6)-6-[1-(4- fluorophenyl)-1H-pyrazol-3-yl]-3- azabicyclo[3.1.0]hexane-3-carboxylate 37 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.357.sup.c 0.027 N.D. 1616 yl (1,5,6)-6-[1-(pyridin-2-ylmethyl)- 1H-pyrazol-3-yl]-3- azabicyclo[3.1.0]hexane-3-carboxylate 38 1,1,1-trifluoro-3-hydroxypropan-2-yl 4- 1.47 0.077 N.D. N.D. [1-(tetrahydro-2H-pyran-4-yl)-1H- pyrazol-3-yl]piperidine-1-carboxylate 39 1,1,1,3,3-pentafluoro-4-hydroxybutan- 0.034 0.002 6.012 9893 2-yl (1,5,6)-6-[1-(4-fluorophenyl)- 1H-pyrazol-3-yl]-3- azabicyclo[3.1.0]hexane-3-carboxylate 40 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.494 0.050 N.D. N.D. yl (3R,6S)-5-[(4- fluorophenyl)sulfonyl]hexahydropyrrolo [3,4-c]pyrrole-2(1H)-carboxylate 41 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 1.58 0.166 N.D. N.D. yl 2-(4-fluorobenzyl)-2,9- diazaspiro[5.5]undecane-9-carboxylate 42 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.304.sup.c 0.032 N.D. 2591 yl 4-(4-fluorobenzyl)-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 43 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- yl 4-[1-(4-fluorophenyl)ethyl]-1-oxa-4,9- 0.145 0.013 >30.0.sup.d 3478 diazaspiro[5.5]undecane-9-carboxylate, formate salt 44 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.179 0.017 >30.0.sup.d 3701 yl 4-[1-(4-fluorophenyl)ethyl]-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate, DIAST-1 45 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.191 0.018 >30.0.sup.d 3134 yl 4-[1-(4-fluorophenyl)ethyl]-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate, DIAST-2 46 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.560 0.046 N.D. N.D. yl 4-{[(4-fluorobenzyl)(tetrahydro-2H- pyran-4-yl)amino]methyl}-4- hydroxpiperidine-1-carboxylate 47 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.174 0.017 >30.0 5046 yl 4-({[(4- fluorophenyl)sulfonyl]amino}methyl) piperidine-1-carboxylate 48 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- >3.00.sup.d 0.926 N.D. N.D. yl (3R,6S)-5-(4-cyclopropylpyridin-2- yl)hexahydropyrrolo[3,4-c]pyrrole- 2(1H)-carboxylate 49 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- >3.00.sup.d 0.654 N.D. N.D. yl 4-(tetrahydro-2H-pyran-4-ylsulfonyl)- 1-oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate 50 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.857 0.071 N.D. N.D. yl 4-{[(4-fluorobenzyl)(tetrahydro-2H- pyran-4-yl)amino]methyl}piperidine-1- carboxylate 51 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.043 0.004 >30.0.sup.d 10340 yl 4-[(4-fluoro-3-methylphenyl)sulfonyl]- 1-oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate 52 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.056 0.005 >30.0.sup.d 7262 yl 4-[(3,4-difluorophenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate 53 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.053 0.006 >30.0 6900 yl 4-{[5-(trifluoromethyl)pyridin-2- yl]oxy}piperidine-1-carboxylate 54 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- yl 4-{[3-(pyrrolidin-1-yl)propyl]sulfonyl}- >3.00.sup.d >1.68 N.D. N.D. 1-oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate, trifluoroacetic acid salt 55 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.447 0.043 N.D. N.D. yl 4-{[2-(pyridin-2-yl)ethyl]sulfonyl}-1- oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate, trifluoroacetic acid salt 56 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 2.34 0.167 N.D. N.D. yl 4-{[3-(1H-imidazol-1- yl)propyl]sulfonyl}-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate, trifluoroacetic acid salt 57 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.126 0.010 N.D. 5998 yl 4-[(5-methylpyridin-2- yl)oxy]piperidine-1-carboxylate 58 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.249 0.033 N.D. N.D. yl 4-{[5-methyl-4-(1-methyl-1H-pyrazol- 5-yl)pyrimidin-2-yl]oxy}piperidine-1- carboxylate 59 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.015 0.001 N.D. 59048 yl 4-[(3-chloro-4-methylphenyl)sulfonyl]- 1-oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate 60 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.037 0.002 N.D. 19870 yl 4-[(3-chloro-4-fluorophenyl)sulfonyl]- 1-oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate 61 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.053 0.005 N.D. 7304 yl 4-[(3,5-dimethylpyridin-2- yl)oxy]piperidine-1-carboxylate 62 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.067.sup.d 0.007.sup.d >30.0.sup.d 216 yl 4-({[(4- fluorophenyl)sulfonyl](methyl)amino} methyl)piperidine-1-carboxylate 63 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.007 0.001 3.01 7347 yl 4-{[3-chloro-5-(trifluoromethyl)pyridin- 2-yl]oxy}piperidine-1-carboxylate 64 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.550 0.042 N.D. N.D. yl 4-{[4-(1-methyl-1H-pyrazol-5- yl)pyridin-2-yl]oxy}piperidine-1- carboxylate 65 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.359 0.030 N.D. N.D. yl 4-{[6-(1-methyl-1H-pyrazol-5- yl)pyridin-2-yl]oxy}piperidine-1- carboxylate 66 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.003 0.0002 7.87.sup.d 430364 yl 4-{[3-(propan-2-yl)phenyl]sulfonyl}-1- oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate 67 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.029 0.004 N.D. 30591 yl 4-(benzylsulfamoyl)-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 68 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.056 0.006 >30.0.sup.d 16586 yl 4-[(4-ethynylphenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate 69 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.104 0.011 N.D. 4621 yl (1,5,6)-6-[1-(6-methoxypyridin-3- yl)-1H-pyrazol-3-yl]-3- azabicyclo[3.1.0]hexane-3-carboxylate 70 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.655 0.069 N.D. N.D. yl 4-[(4-fluorophenyl)sulfonyl]-5-methyl- 1-oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate, DIAST-1 71 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.099 0.017 N.D. 9358 yl 4-[(4-fluorophenyl)sulfonyl]-5-methyl- 1-oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate, DIAST-2 72 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.220 0.028 N.D. N.D. yl 3-(4-fluorobenzyl)-2-oxo-1-oxa-3,8- diazaspiro[4.5]decane-8-carboxylate 73 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.005 0.001 >30.0.sup.d 96515 yl 4-[(3-fluoro-4-methylphenyl)sulfonyl]- 1-oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate 74 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.392 0.052 N.D. N.D. yl 4-[(pyridin-2-ylmethyl)sulfamoyl]-1- oxa-4,9-diazaspiro[5.5]undecane-9- carboxylate 75 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.688 0.079 N.D. N.D. yl 4-{[5-(hydroxymethyl)pyridin-2- yl]oxy}piperidine-1-carboxylate 76 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.658 0.083 N.D. N.D. yl 4-(5-methylpyrimidin-2-yl)-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 77 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.005 0.001 5.95.sup.d 403739 yl 4-[(3-ethylphenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 78 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.006 0.001 2.58.sup.d 16865 yl 4-{[4-(propan-2- yloxy)phenyl]sulfonyl}-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 79 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.007 0.001 10.0 26513 yl 4-[(3-ethynylphenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate 80 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.007 0.001 7.92.sup.d 126124 yl 4-[1-(4-ethynylphenyl)-1H-pyrazol-3- yl]piperidine-1-carboxylate 81 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.008 0.001 7.54.sup.d 45138 yl (1,5,6)-6-[1-(4-ethynylphenyl)-1H- pyrazol-3-yl]-3- azabicyclo[3.1.0]hexane-3-carboxylate 82 (2S)-1,1,1-trifluoro-3-hydroxpropan-2- >2.75 0.598 N.D. N.D. yl (1,5,6)-6-[1-(4-ethynylphenyl)-1H- pyrazol-3-yl]-3- azabicyclo[3.1.0]hexane-3-carboxylate 83 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.019.sup.c 0.002.sup.c >30.0.sup.d 21997.sup.d yl 4-[(3-chlorophenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate 84 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.026 0.002 19.2.sup.d 49166 yl 4-[(2-fluorophenyl)sulfonyl]-1-oxa- 4,9-diazaspiro[5.5]undecane-9- carboxylate 85 methyl(2R)-3,3,3-trifluoro-2-[({4-[(4- >3.00.sup.d 0.818 N.D. N.D. fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undec-9- yl}carbonyl)oxy]propyl phosphate, ammonium salt 86 (2R)-3-[(dimethoxyphosphoryl)oxy]- 0.097 0.008 N.D. 132 1,1,1-trifluoropropan-2-yl 4-[(4- fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 87 ethyl(2R)-3,3,3-trifluoro-2-[({4-[(4- 0.198 0.015 N.D. N.D. fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undec-9- yl}carbonyl)oxy]propyl phosphate, ammonium salt 88 (2R)-3-[(diethoxyphosphoryl)oxy]-1,1,1- >3.00.sup.d >3.00.sup.d N.D. N.D. trifluoropropan-2-yl 4-[(4- fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 89 (9R)-10,10,10-trifluoro-6-hydroxy-2- >3.00.sup.d >3.00.sup.d N.D. N.D. methyl-6-oxido-5,7-dioxa-2-aza-6.sup.5- phosphadecan-9-yl 4-[(4- fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undecane-9-carboxylate 90 (2R)-3,3,3-trifluoro-2-[({4-[(4- >3.00.sup.d >3.00.sup.d N.D. N.D. fluorophenyl)sulfonyl]-1-oxa-4,9- diazaspiro[5.5]undec-9- yl}carbonyl)oxy]propyl 2- (trimethylammonio)ethyl phosphate 91 (2R)-3,3,3-trifluoro-2-({[4- N.D. N.D. N.D. N.D. (phenylsulfonyl)-1-oxa-4,9- diazaspiro[5.5]undec-9- yl]carbonyl}oxy)propyl phosphate, disodium salt 92 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.006 0.001 >30.sup.d 215500 yl (3R)-3-[ethyl(phenylsulfonyl)amino]- 1-oxa-8-azaspiro[4.5]decane-8- carboxylate 93 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.318.sup.c 0.029.sup.c >30.sup.d 3282 yl (3R)-3- [(cyclopropylsulfonyl)(methyl)amino]-1- oxa-8-azaspiro[4.5]decane-8- carboxylate 94 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.316.sup.c 0.027.sup.c >30.sup.d 3764 yl (3S)-3- [(cyclopropylsulfonyl)(methyl)amino]-1- oxa-8-azaspiro[4.5]decane-8- carboxylate 95 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.016 0.001 8.72 131495 yl 3-phenyl-1-oxa-8- azaspiro[4.5]decane-8-carboxylate [From C101, ENT-2] 96 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.062 0.005 9.52 18560 yl 3-phenyl-1-oxa-8- azaspiro[4.5]decane-8-carboxylate [From C100, ENT-1] 97 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.192 0.020 >30.sup.d 1897 yl 3-(5-fluoropyridin-2-yl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 98 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.071.sup.c 0.007.sup.c >30.sup.d 4933 yl 2-(2-fluorobenzoyl)-2,8- diazaspiro[4.5]decane-8-carboxylate 99 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.107 0.011 >30.sup.d 16883 yl 3-[benzoyl(methyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate [From C112, DIAST-2] 100 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.086 0.010 >28.7 5664 yl 3-[benzoyl(methyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate [From C111, DIAST-1] 101 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.180 0.021 6.54 2454 yl 3-(1,1-dioxido-1,2-benzothiazol- 2(3H)-yl)-1-oxa-8-azaspiro[4.5]decane- 8-carboxylate 102 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.215 0.026 18.3 1336 yl 3-[(5-methylpyridin-2-yl)methyl]-1- oxa-8-azaspiro[4.5]decane-8- carboxylate 103 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.334 0.030 >30.sup.d N.D. yl 3-(1H-pyrazol-1-yl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate [From C120, DIAST-2] 104 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 1.86 0.157 N.D. N.D. yl 3-(1H-pyrazol-1-yl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate [From C119, DIAST-1] 105 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.022 0.002 9.76 59993 yl 2-(phenylsulfonyl)-2,8- diazaspiro[4.5]decane-8-carboxylate 106 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.238 0.020 >30.sup.d 2856 yl (3R)-3- {[(cyclopropylmethyl)sulfonyl](methyl) amino}-1-oxa-8-azaspiro[4.5]decane-8- carboxylate 107 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.010 0.001 >30.sup.d 127771 yl (3R)-3-[(phenylsulfonyl)(propan-2- yl)amino]-1-oxa-8-azaspiro[4.5]decane- 8-carboxylate 108 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.051 0.003 10.7 15960 yl 3-{[(3- fluorophenyl)sulfonyl](methyl)amino}-1- oxa-8-azaspiro[4.5]decane-8- carboxylate, DIAST-1 109 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.021 0.002 >30.sup.d 34918 yl 3-{[(3- fluorophenyl)sulfonyl](methyl)amino}-1- oxa-8-azaspiro[4.5]decane-8- carboxylate, DIAST-2 110 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.070 0.006 7.09 8796 yl 3-{[(4- fluorophenyl)sulfonyl](methyl)amino}-1- oxa-8-azaspiro[4.5]decane-8- carboxylate, DIAST-1 111 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.028 0.002 >30.sup.d 33483 yl 3-{[(4- fluorophenyl)sulfonyl](methyl)amino}-1- oxa-8-azaspiro[4.5]decane-8- carboxylate, DIAST-2 112 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.064 0.007 >30.sup.d 14232 yl 3- [(cyclopentylsulfonyl)(methyl)amino]-1- oxa-8-azaspiro[4.5]decane-8- carboxylate 113 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.139 0.010 >25.5 6254 yl 3-[(cyclobutylsulfonyl)(methyl)amino]- 1-oxa-8-azaspiro[4.5]decane-8- carboxylate 114 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.163 0.015 >30.sup.d 6263 yl 3-[methyl(pyridin-3-ylsulfonyl)amino]- 1-oxa-8-azaspiro[4.5]decane-8- carboxylate 115 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.341 0.027 >30.sup.d 2917 yl 3-[methyl(pyrimidin-5- ylsulfonyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 116 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.986 0.084 N.D. N.D. yl 3-[methyl(tetrahydro-2H-pyran-4- ylsulfonyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate [From DIAST-2 in footnote 43, Table 6] 117 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 1.97 0.153 N.D. N.D. yl 3-[methyl(tetrahydro-2H-pyran-4- ylsulfonyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate [From DIAST-1 in footnote 43, Table 6] 118 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.002 0.0004 >24.8 172844 yl 3-[3-(trifluoromethoxy)phenyl]-1-oxa- 8-azaspiro[4.5]decane-8-carboxylate 119 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.032 0.003 3.57 23176 yl 3-(3-chlorophenyl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 120 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.166 0.021 >30.sup.d 1516 yl 3-(6-fluoropyridin-3-yl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 121 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.149 0.020 14.7 1207 yl 3-(5-methoxypyridin-2-yl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 122 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.385 0.032 N.D. N.D. yl 3-(pyridin-3-yl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate, trifluoroacetate salt 123 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.349 0.030 24.6 N.D. yl 3-(6-methylpyridin-3-yl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate, trifluoroacetate salt 124 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.092 0.010 >30.sup.d 6720 yl 2-(cyclopentylcarbonyl)-2,8- diazaspiro[4.5]decane-8-carboxylate 125 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.065 0.007 >30.sup.d 5464 yl 2-(1,3-thiazol-2-ylcarbonyl)-2,8- diazaspiro[4.5]decane-8-carboxylate 126 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.112 0.012 >30.sup.d 3723 yl 2-benzoyl-2,8-diazaspiro[4.5]decane- 8-carboxylate 127 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.163 0.017 >30.sup.d 2067 yl 2-[(4,4-difluorocyclohexyl)carbonyl]- 2,8-diazaspiro[4.5]decane-8- carboxylate 128 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.423 0.050 N.D. N.D. yl 2-(pyridin-2-ylcarbonyl)-2,8- diazaspiro[4.5]decane-8-carboxylate 129 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- >3.sup.d 0.474 N.D. N.D. yl 3-(pyridin-2-yloxy)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate, DIAST-1 130 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.076 0.006 25.7 12483 yl 3-(pyridin-2-yloxy)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate, DIAST-2 131 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.086 0.011 17.4 4241 yl 3-(1,1-dioxido-3,4-dihydro-2H-1,2- benzothiazin-2-yl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 132 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.130 0.012 N.D. 8426 yl 3-(benzyloxy)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 133 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.174 0.020 4.75 3614 yl 2-(pyrrolidin-1-ylcarbonyl)-2,8- diazaspiro[4.5]decane-8-carboxylate 134 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.096 0.007 >23.4 9930 yl 2-(1,3-thiazol-2-ylsulfonyl)-2,8- diazaspiro[4.5]decane-8-carboxylate 135 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.168 0.013 >30.sup.d 4723 yl 2-(pyridin-3-ylsulfonyl)-2,8- diazaspiro[4.5]decane-8-carboxylate 136 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.052 0.005 >30.sup.d 15516 yl (3R)-3-[methyl(1,3-thiazol-2- ylsulfonyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 137 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.126 0.011 >30.sup.d 7582 yl (3R)-3-{methyl[(2,2,2- trifluoroethyl)sulfonyl]amino}-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 138 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.185 0.015 16.3 2154 yl (3R)-3-{methyl[(1-methyl-1H-pyrazol- 4-yl)sulfonyl]amino}-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 139 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.153 0.015 >30.sup.d 3058 yl (3R)-3-{methyl[(2- methylpropyl)sulfonyl]amino}-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 140 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.094 0.009 >30.sup.d 4595 yl (3R)-3- {[(cyclobutylmethyl)sulfonyl](methyl) amino}-1-oxa-8-azaspiro[4.5]decane-8- carboxylate 141 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.077 0.007 >30.sup.d 3519 yl (3R)-3-[(3- fluorobenzoyl)(methyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 142 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.084 0.009 >30.sup.d 3961 yl (3R)-3- [(cyclobutylcarbonyl)(methyl)amino]-1- oxa-8-azaspiro[4.5]decane-8- carboxylate 143 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.096 0.010 >30.sup.d 2585 yl (3R)-3- [(cyclobutylacetyl)(methyl)amino]-1- oxa-8-azaspiro[4.5]decane-8- carboxylate 144 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.105 0.010 >30.sup.d 2802 yl (3R)-3-[methyl(3- methylbutanoyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 145 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.198 0.019 >30.sup.d 1476 yl (3R)-3- [(cyclopropylacetyl)(methyl)amino]-1- oxa-8-azaspiro[4.5]decane-8- carboxylate 146 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.539 0.048 N.D. N.D. yl (3R)-3-[methyl(pyridin-2- ylcarbonyl)amino]-1-oxa-8- azaspiro[4.5]decane-8-carboxylate, formate salt 147 (2R)-1,1,1-trifluoro-3-hydroxypropan-2- 0.060 0.006 >26.4 5973 yl 3-(3-cyanophenyl)-1-oxa-8- azaspiro[4.5]decane-8-carboxylate 148 (2R)-3,3,3-trifluoro-2-[({(3R)-3- >3.sup.d >1.23 N.D. N.D. [methyl(phenylsulfonyl)amino]-1-oxa-8- azaspiro[4.5]dec-8- yl}carbonyl)oxy]propyl phosphate, (bis)- L-lysine salt 149 (2R)-3,3,3-trifluoro-2-({[4- N.D. N.D. N.D. N.D. (phenylsulfonyl)-1-oxa-4,9- diazaspiro[5.5]undec-9- yl]carbonyl}oxy)propyl phosphate,(bis)- L-lysine salt 150 (2R)-3,3,3-trifluoro-2-({[(3R)-3-{[(4- N.D. N.D. N.D. N.D. fluorophenypsulfonyl](methyl)amino}-1- oxa-8-azaspiro[4.5]dec-8- yl]carbonyl}oxy)propyl phosphate,(bis)- L-lysine salt .sup.aReported IC.sub.50 values or k.sub.inact/K.sub.I values are the geometric mean of 2-4 determinations, unless otherwise indicated. .sup.bN.D. = not determined .sup.cThe reported IC.sub.50 value or k.sub.inact/K.sub.I value is the geometric mean of 5 determinations. .sup.dThe IC.sub.50 value or k.sub.inact/K.sub.I value is from a single determination. .sup.eIn this case, the corresponding phosphate itself was tested, rather than the salt.

Example BB: Prodrug In Vivo Data

(365) Rats

(366) Test compounds (Examples 31 and 32) were administered intravenously to groups of two rats. The characteristics of the experimental rats are given in Table BB-1.

(367) TABLE-US-00009 TABLE BB-1 Characteristics of experimental rats used in study Species Rats Type Wistar Hann Number and sex 2 males Approximate age 7-11 weeks Approx. Body weight 250-320 g at start of treatment Source Charles River Labs

(368) Blood samples were taken at various times after administration and submitted to analysis for the parent compound (Examples 9 or 6) and prodrug compound (Examples 31 or 32, respectively) using an LC-MS-MS assay. Pharmacokinetic parameters derived from the plasma analytical data were determined using Watson LIMS 7.2.003 (Thermo Fisher Scientific, Waltham, Mass.). The results are given in Tables BB-2 to BB-5.

(369) TABLE-US-00010 TABLE BB-2 Pharmacokinetic Parameters of Example 31 in Wistar Hann Rats Following IV Administration at 1.48 mg/kg Subject Subject Parameter Units Rat 01 Rat 02 Mean S.D Original Dose mg/kg 1.48 1.48 (Example 31) AUC Interval (0-0.5 (0-0.25 Hours) Hours) AUC ng*Hours/mL 43.1 42.9 43.0 AUC Extrap ng*Hours/mL 43.6 43.6 43.6 % AUC Extrap % 1.19 1.62 1.41 Co ng/mL 399 553 476 CL mL/min/kg 566 566 566 T Hours 0.0805 0.0445 0.0625 Vdss L/kg 2.86 1.59 2.23 Rate Constant 1/Hours 8.61 15.6 12.1 Regression Hours 0.083, 0.083, 0.25 Points 0.25, 0.5

(370) TABLE-US-00011 TABLE BB-3 Pharmacokinetic Parameters of Example 9 in Wistar Hann Rats Following IV Administration of Example 31 at 1.48 mg/kg Subject Subject Parameter Units Rat 01 Rat 02 Mean S.D. Original Dose mg/kg 1.48 1.48 (Example 31) Cmax ng/mL 253 378 316 Tmax Hours 0.083 0.083 0.083 AUC ng*Hours/mL 118 173 146 AUC Extrap ng*Hours/mL 121 178 150 % AUC Extrap % 2.08 2.83 2.46 Rate Constant 1/Hours 0.560 0.440 0.500 T Hours 1.24 1.57 1.41 Regression Points Hours 4, 7 1, 2, 4, 7

(371) TABLE-US-00012 TABLE BB-4 Pharmacokinetics of Example 32 in rats after IV administration of Example 32 (2 mg/kg active) Subject Subject Parameter Units Rat 03 Rat 04 Mean S.D. Original Dose mg/kg 2 2 (Example 32) AUC Interval (0-1 (0-0.5 Hours) Hours) AUC ng*Hours/mL 185 133 159 AUC Extrap ng*Hours/mL 185 134 160 % AUC Extrap % 0.232 0.832 0.532 Co ng/mL 4480 3040 3760 CL mL/min/kg 180 249 215 T Hours 0.147 0.0971 0.122 Vdss L/kg 0.515 0.679 0.597 Rate Constant 1/Hours 4.73 7.14 5.94 Regression Hours 0.5, 1 0.25, 0.5 Points

(372) TABLE-US-00013 TABLE BB-5 Pharmacokinetic Parameters of Example 6 in Wistar Hann Rats Following IV Administration of Example 32 at 2 mg/kg Subject Subject Parameter Units Rat 03 Rat 04 Mean S.D. Original Dose mg/kg 2 2 (Example 32) Cmax ng/mL 234 384 309 Tmax Hours 0.083 0.033 0.058 AUC ng*Hours/mL 102 213 158 AUC Extrap ng*Hours/mL 109 215 162 % AUC Extrap % 6.04 0.880 3.46 Rate Constant 1/Hours 2.86 1.57 2.22 T Hours 0.242 0.442 0.342 Regression Hours 0.25, 0.5, 1 0.5, 1,3 Points
Dogs

(373) Test compounds (Examples 31 and 32) were administered intravenously to groups of two dogs. The characteristics of the experimental dogs are given in Table BB-6.

(374) TABLE-US-00014 TABLE BB-6 Characteristics of experimental dogs used in study Species Dogs Type Beagle Number and sex 2 males Approximate age 2-5 years Approx. Body weight 9-13 kg at start of treatment Source Marshall Farms

(375) Blood samples were taken at various times after administration and submitted to analysis for the parent compound (Example 9 or 6) and its prodrug compound (Example 31 or 32, respectively) using an LC-MS-MS assay. Pharmacokinetic parameters derived from the plasma analytical data were determined using Watson LIMS 7.2.003 (Thermo Fisher Scientific, Waltham, Mass.). The results are given in Tables BB-7 to BB-10.

(376) TABLE-US-00015 TABLE BB-7 Pharmacokinetic Parameters of Example 31 in Beagle Dogs Following IV Administration at 0.7 mg/kg Subject Subject Parameter Units Dog 01 Dog 02 Mean S.D. Original Dose mg/kg 0.7 0.7 (Example 31) AUC Interval (0-0.5 (0-0.5 Hours) Hours) AUC ng*Hours/mL 108 53.8 80.9 AUC Extrap ng*Hours/mL 108 53.9 81.0 % AUC % 0.181 0.103 0.142 Extrap Co ng/mL 1630 821 1230 CL mL/min/kg 108 216 162 T Hours 0.0614 0.0620 0.0617 Vdss L/kg 0.235 0.465 0.350 Rate Constant 1/Hours 11.3 11.2 11.3 Regression Hours 0.083, 0.083, Points 0.25, 0.5 0.25, 0.5

(377) TABLE-US-00016 TABLE BB-8 Pharmacokinetic Parameters of Example 9 in Beagle Dogs Following IV Administration of Example 31 at 0.7 mg/kg Subject Subject Parameter Units Dog 01 Dog 02 Mean S.D. Original Dose mg/kg 0.7 0.7 (Example 31) Cmax ng/mL 614 789 702 Tmax Hours 0.25 0.083 0.17 AUC ng*Hours/mL 1350 1460 1410 AUC Extrap ng*Hours/mL 1550 1560 1560 % AUC Extrap % 12.6 6.12 9.36 Rate Constant 1/Hours 0.0648 0.0863 0.0756 T Hours 10.7 8.03 9.37 Regression Points Hours 4, 7, 24 2, 4, 7, 24

(378) TABLE-US-00017 TABLE BB-9 Pharmacokinetic Parameters of Example 32 in Beagle Dogs Following IV Administration at 1 mg/kg Subject Subject Parameter Units Dog 03 Dog 04 Mean S.D. Original Dose mg/kg 1 1 (Example 32) AUC Interval (0-1 Hours) (0-1 Hours) AUC ng*Hours/mL 146 229 188 AUC Extrap ng*Hours/mL 146 229 188 % AUC % 0.0443 0.150 0.0972 Extrap Co ng/mL 1220 2370 1800 CL mL/min/kg 114 72.8 93.4 T Hours 0.136 0.137 0.137 Vdss L/kg 0.751 0.357 0.554 Rate 1/Hours 5.08 5.06 5.07 Constant Regression Hours 0.25, 0.5, 1 0.25, 0.5, 1 Points

(379) TABLE-US-00018 TABLE BB-10 Pharmacokinetic Parameters of Example 6 in Beagle Dogs Following IV Administration of Example 32 at 1 mg/kg Subject Subject Parameter Units Dog 03 Dog 04 Mean S.D. Original Dose mg/kg 1 1 (Example 32) Cmax ng/mL 514 653 584 Tmax Hours 0.083 0.083 0.083 AUC ng*Hours/mL 591 705 648 AUC Extrap ng*Hours/mL 595 710 653 % AUC Extrap % 0.630 0.733 0.682 Rate Constant 1/Hours 0.169 0.129 0.149 T Hours 4.10 5.36 4.73 Regression Hours 4, 7, 24 7, 24 Points

(380) Various modifications of the invention, in addition to those described herein, will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appendant claims. Each reference (including all patents, patent applications, journal articles, books, and any other publications) cited in the present application is hereby incorporated by reference in its entirety.