Preparation Method of the Albumin Peptide Combination and the Action of Inhibiting the Proliferation of Cancer Cells Thereof

Abstract

The present invention discloses a preparation method of an albumin peptide combination and the action of inhibiting the proliferation of cancer cells thereof, the preparation steps comprise: mixing albumin and water in proportion, heating, adjusting pH, adding alkaline protease to perform enzymatic hydrolysis, deactivating, filtrating; mixing coix seed and water in proportion, decocting to extract, filtrating, adding certain proportional of water to filter residue, continue decocting to extract, collecting filtrates, determining solid matter content respectively, mixing two solutions, concentrating, spray drying, and obtaining an albumin peptide combination having the action of inhibiting the proliferation of cancer cells. The present invention found that, as compared with using coix seed individually, the effect of inhibiting the proliferation of cancer cells of albumin peptide combination is stronger, and process is simpler.

Claims

1. An albumin peptide combination having an action of inhibiting a proliferation of cancer cells, comprising: raw materials including an albumin enzymatic hydrolysate and a coix seed extract under mass ratio of 5:2 to 5, wherein a content of a solid matter of the albumin enzymatic hydrolysate is 15% to 35% and a content of a solid matter of the coix seed extract is 10% to 30%.

2. The albumin peptide combination, as recited in claim 1, wherein the albumin enzymatic hydrolysate is produced by a preparation method comprising the steps of: (a1) adding albumin into water that is 5 to 20 times to a mass thereof, heating the albumin and water mixture to 40° C. to 50° C., and homogenizing for producing a solution; (a2) adjusting pH of the solution after the homogenizing of the step (a1) to 7 to 9, adding an alkaline protease at 3% to 5% of the mass of the solution to perform enzymatic hydrolysis reaction for 5 h to 10 h for producing a hydrolysis solution; (a3) heating the hydrolysis solution of the step (a2) to boil, and deactivating for 0.5 h to 2 h; and (a4) cooling the hydrolysis solution after the deactivating of the step (a3) to 60° C. to 80° C., and performing filtration to obtain the albumin enzymatic hydrolysate.

3. The albumin peptide combination, as recited in claim 2, wherein the filtration process of the step (a4) is plate frame filtration.

4. The albumin peptide combination, as recited in claim 1, wherein the coix seed extract is produced by a preparation method comprising the steps of: (b1) decocting first coix seeds with boiling water for 3 h to 5 h, wherein an amount of water is 10 to 15 times of a mass of the first coix seeds, subsequently, performing filtration; (b2) decocting second coix seeds with boiling water for 1 h to 3 h, wherein an amount of water is 5 to 10 times of a mass of the second coix seeds, subsequently, performing filtration; and (b3) combining filtrates of the two filtrations of the step (b1) and the step (b2) to obtain the coix seed extract.

5. The albumin peptide combination, as recited in claim 4, wherein the filtration process in the steps (b1) and (b2) is plate frame filtration.

6. The albumin peptide combination, as recited in claim 1, wherein said albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

7. The albumin peptide combination, as recited in claim 2, wherein said albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

8. The albumin peptide combination, as recited in claim 3, wherein said albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

9. The albumin peptide combination, as recited in claim 4, wherein said albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

10. The albumin peptide combination, as recited in claim 5, wherein said albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

11. A preparation method of an albumin peptide combination having the action of inhibiting the proliferation of cancer cells, wherein the albumin peptide combination, having an action of inhibiting a proliferation of cancer cells, comprising: raw materials including an albumin enzymatic hydrolysate and a coix seed extract under mass ratio of 5:2 to 5, wherein a content of a solid matter of the albumin enzymatic hydrolysate is 15% to 35% and a content of a solid matter of the coix seed extract is 10% to 30%, wherein the preparation method comprises the steps of: (1) adding albumin into water that is 5 to 20 times to a mass thereof, heating the albumin and water mixture to 40° C. to 50° C., and homogenizing for producing a solution; (2) adjusting pH of the solution after the homogenizing of step (1) to 7 to 9, adding an alkaline protease at 3% to 5% of the mass of the solution to perform enzymatic hydrolysis reaction for 5 h to 10 h for producing a hydrolysis solution; (3) heating the hydrolysis solution of the step (2) to boil, deactivating for 0.5 h to 2 h; (4) cooling the hydrolysis solution after the deactivating of the step (3) to 60° C. to 80° C., and performing filtration to obtain the albumin enzymatic hydrolysate; (5) decocting first coix seeds with boiling water for 3 h to 5 h, wherein an amount of water is 10 to 15 times of a mass of the first coix seeds, subsequently, performing filtration; (6) decocting second coix seeds with boiling water for 1 h to 3 h, wherein an amount of water is 5 to 10 times of a mass of the second coix seed, subsequently, performing filtration; (7) combining filtrates of the two filtrations of the step (5) and the step (6) to obtain the coix seed extract; (8) performing solid matter determination for the filtrate of the albumin peptide enzymatic hydrolysate and the coix seed extract respectively, mixing the albumin peptide enzymatic hydrolysate and the coix seed extract under appropriate ratio; and (9) performing low temperature vacuum concentration to the mixed liquid of the step (8), wherein temperature is 40° C. to 60° C. and pressure is 0.03 MPa to 0.07 MPa, subsequently, spray drying, wherein the temperature of the wind at inlet is 180° C. to 220° C. and the temperature of the wind at outlet is 80° C. to 110° C., preparing the albumin peptide combination having the action of inhibiting the proliferation of cancer cells.

12. The preparation method, as recited in claim 11, wherein the step (1) to the step (4) are preparation process to produce the albumin enzymatic hydrolysate.

13. The preparation method, as recited in claim 12, wherein the step (4) is a filtration process that is a plate frame filtration.

14. The preparation method, as recited in claim 11, wherein the step (5) to the step (7) are preparation process to produce the coix seed extract.

15. The preparation method, as recited in claim 14, wherein the step (5) and the step (6) are filtration process which is plate frame filtration.

16. The preparation method, as recited in claim 11, wherein all the filtration processes are plate frame filtration.

17. The preparation method, as recited in claim 11, wherein the albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

18. The preparation method, as recited in claim 12, wherein the albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

19. The preparation method, as recited in claim 14, wherein the albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

20. The preparation method, as recited in claim 16, wherein the albumin peptide combination is produced in form of the group consisting of powders, granules, tablets, capsules, and oral solution.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0036] FIG. 1 is a process flow chart of the preparation method of the albumin peptide combination having the action of inhibiting the proliferation of cancer cells of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

[0037] The following description is disclosed to enable any person skilled in the art to make and use the present invention. Preferred embodiments are provided in the following description only as examples and modifications will be apparent to those skilled in the art. The general principles defined in the following description would be applied to other embodiments, alternatives, modifications, equivalents, and applications without departing from the spirit and scope of the present invention.

[0038] The followings are the illustration performed to the preferable examples of the present application, it shall be understood that, the preferable examples described herein are merely used to illustrate and explain the present invention, but not used to define the present invention.

Example 1 an Albumin Peptide Combination Having the Action of Inhibiting the Proliferation of Cancer Cells

[0039] The preparation method comprises each step as follows:

[0040] (1) adding an albumin into water that is 20 times to the mass thereof, heating the albumin and water mixture to 50° C., and homogenizing for producing a solution;

[0041] (2) adjusting the acidity of the solution after the homogenizing of step (1) to pH 8 by dilute sodium hydroxide, adding an alkaline protease at 3% of the mass of the solution, performing enzymatic hydrolysis for 8 h for producing hydrolysate;

[0042] (3) heating the hydrolysate after the enzymatic hydrolysis to boil, deactivating for 0.5 h;

[0043] (4) cooling the hydrolysate after the deactivating of step (3) to 60° C., performing plate frame filtration.

[0044] (5) decocting first coix seeds with boiling water for 3 h, wherein the amount of water is 10 times of the mass of the first coix seeds, performing plate frame filtration;

[0045] (6) decocting second coix seeds with boiling water for 1 h, wherein the amount of water is 5 times of the mass of the second coix seeds, performing plate frame filtration;

[0046] (7) combining the filtrates of the two filtrations from step (5) and step (6);

[0047] (8) performing solid matter determination for the filtrate of the albumin peptide enzymatic hydrolysate and the coix seed extract respectively, wherein the content of the solid matter of the albumin enzymatic hydrolysate is 17.3% and the content of the solid matter of the coix seed extract is 22.8%, mixing the two under ratio of 5:3 by total weight;

[0048] (9) performing low temperature vacuum concentration to the mixed liquid, wherein temperature is 60° C. and pressure is 0.07 MPa, spray drying to prepare final product, wherein the temperature of the wind at inlet is 200° C. and the temperature of the wind at outlet is 90° C.

[0049] In the final product, the content of polypeptide is greater than 38.7%, the content of total carbohydrate is greater than 21.1%, and moisture 4.1%, which meets the requirement in the product standard that the content of polypeptide is greater than 20% and the content of total carbohydrate is greater than 10%.

Example 2 a Preparation of the Albumin Peptide Combination Having the Action of Inhibiting the Proliferation of Cancer Cells

[0050] The preparation method comprises each step as follows:

[0051] (1) adding an albumin into water that is 10 times to the mass thereof, heating the albumin and water mixture to 50° C., homogenizing for producing a solution;

[0052] (2) adjusting the acidity of the solution after the homogenizing of step (1) to pH 9 by dilute sodium hydroxide, adding an alkaline protease at 4% of the mass of the solution, performing enzymatic hydrolysis for 6 h for producing hydrolysate;

[0053] (3) heating the hydrolysate after the enzymatic hydrolysis to boil, deactivating for 0.5 h;

[0054] (4) cooling the hydrolysate after the deactivating of step (3) to 60° C., performing plate frame filtration.

[0055] (5) decocting first coix seeds with boiling water for 5 h, wherein the amount of water is 15 times of the mass of the first coix seeds, performing plate frame filtration;

[0056] (6) decocting second coix seeds with boiling water for 3 h, wherein the amount of water is 10 times of the mass of the second coix seeds, performing plate frame filtration;

[0057] (7) combining the filtrates of the two filtrations from step (5) and step (6);

[0058] (8) performing solid matter determination for the filtrate of the albumin peptide enzymatic hydrolysate and the coix seed extract respectively, wherein the content of the solid matter of the albumin enzymatic hydrolysate is 31.3% and the content of the solid matter of the coix seed extract is 21.9%, mixing the two under ratio of 5:4 by total weight;

[0059] (9) performing low temperature vacuum concentration to the mixed liquid, wherein temperature is 60° C. and pressure is 0.03 MPa, spray drying to prepare final product, wherein the temperature of the wind at inlet is 200° C. and the temperature of the wind at outlet is 90° C.

[0060] In the final product, the content of polypeptide is greater than 39.0%, the content of total carbohydrate is greater than 20.3%, and moisture 4.5%, which meets the requirement in the product standard that the content of polypeptide is greater than 20% and the content of total carbohydrate is greater than 10%.

Example 3 a Preparation of the Albumin Peptide Combination Having the Action of Inhibiting the Proliferation of Cancer Cells

[0061] The preparation method comprises each step as follows:

[0062] (1) adding an albumin into water that is 10 times to the mass thereof, heating the albumin and water mixture to 50° C., homogenizing for producing a solution;

[0063] (2) adjusting the acidity of the solution after the homogenizing of step (1) to pH 9 by dilute sodium hydroxide, adding an alkaline protease at 3% of the mass of the solution, performing enzymatic hydrolysis for 7 h for producing hydrolysate;

[0064] (3) heating the hydrolysate after the enzymatic hydrolysis to boil, deactivating for 0.5 h;

[0065] (4) cooling the hydrolysate after the deactivating of step (3) to 60° C., performing plate frame filtration.

[0066] (5) decocting first coix seeds with boiling water for 4 h, wherein the amount of water is 13 times of the mass of the first coix seeds, performing plate frame filtration;

[0067] (6) decocting second coix seeds with boiling water for 2 h, wherein the amount of water is 8 times of the mass of the second coix seeds, performing plate frame filtration;

[0068] (7) combining the filtrates of the two filtrations from step (5) and step (6);

[0069] (8) performing solid matter determination for the filtrate of the albumin peptide enzymatic hydrolysate and the coix seed extract respectively, wherein the content of the solid matter of the albumin enzymatic hydrolysate is 30.9% and the content of the solid matter of the coix seed extract is 21.3%, mixing the two under ratio of 5:4 by total weight;

[0070] (9) performing low temperature vacuum concentration to the mixed liquid, wherein temperature is 60° C. and pressure is 0.03 MPa; spray drying to prepare final product, wherein the temperature of the wind at inlet is 205° C. and the temperature of the wind at outlet is 93° C.

[0071] In the final product, the content of polypeptide is greater than 38.8%, the content of total carbohydrate is greater than 20.2%, and moisture 4.3%, which meets the requirement in the product standard that the content of polypeptide is greater than 20% and the content of total carbohydrate is greater than 10%.

Example 4 a Preparation of the Albumin Peptide Combination Having the Action of Inhibiting the Proliferation of Cancer Cells

[0072] The preparation method comprises each step as follows:

[0073] (1) adding an albumin into water that is 5 times to the mass thereof, heating the albumin and water mixture to 40° C., homogenizing for producing a solution;

[0074] (2) adjusting the acidity of the solution after the homogenizing of step (1) to pH 7 by dilute sodium hydroxide, adding an alkaline protease at 5% of the mass of the solution, performing enzymatic hydrolysis for 10 h for producing hydrolysate;

[0075] (3) heating the hydrolysate after the enzymatic hydrolysis to boil, deactivating for 2 h;

[0076] (4) cooling the hydrolysate after the deactivating of step (3) to 60° C., performing plate frame filtration.

[0077] (5) decocting first coix seeds with boiling water for 4 h, wherein the amount of water is 13 times of the mass of the first coix seeds, performing plate frame filtration;

[0078] (6) decocting second coix seeds with boiling water for 2 h, wherein the amount of water is 8 times of the mass of the second coix seeds, performing plate frame filtration;

[0079] (7) combining the filtrates of the two filtrations from step (5) and step (6);

[0080] (8) performing solid matter determination for the filtrate of the albumin peptide enzymatic hydrolysate and the coix seed extract respectively, wherein the content of the solid matter of the albumin enzymatic hydrolysate is 15% and the content of the solid matter of the coix seed extract is 10%, mixing the two under ratio of 5:2 by total weight;

[0081] (9) performing low temperature vacuum concentration to the mixed liquid, wherein temperature is 40° C. and pressure is 0.03 MPa, spray drying to prepare final product, wherein the temperature of the wind at inlet is 180° C., the temperature of the wind at outlet is 110° C.

[0082] In the final product, the content of polypeptide is greater than 38.0%, the content of total carbohydrate is greater than 20.0%, and moisture 4.1%, which meets the requirement in the product standard that the content of polypeptide is greater than 20% and the content of total carbohydrate is greater than 10%.

Example 5 the Inhibition Experiment of Different Albumin Peptide Combination Solutions to Hepatoma Cells

[0083] Preparing and obtaining tested sample group solutions with concentrations of low dose group 10 mg/L, medium dose group 30 mg/L and high dose group 50 mg/L respectively, according to the albumin peptide combination obtained by the process of Example 3.

[0084] Preparing the coix seed extract solution with concentration of 13.3 mg/L as control group, according to the preparation method of the process of the coix seed extract in Example 3.

[0085] Taking Cell Culture Solution as Blank Group.

[0086] Taking the human hepatoma cell SMMC-7721 in logarithmic growth phase, pouring out culture solution, adding 0.25% pancreatin solution, after digesting under 37° C. for 2-3 min, observing cells under inverted microscope, pouring out digesting solution when 80% cells become round. Adding culture solution 4 mL having the addition amount of 20% calf serum, pipetting repeatedly, to make all cells washed down, pipetting slightly to mix homogeneously, preparing single cell suspension, adjusting culture solution to make the concentration of cell to be 105/mL, taking 105/mL cell suspension, inoculating 96 wells plate at 195 μL per well, after culturing for 24 h, adding 5 μL for each of tested sample solutions of different doses as well as blank group and control group solution, setting 6 parallel wells for each. After culturing for 2 days, adding 5 mg/mL MTT 20 μL per well, continue culturing for 4 h, sucking out culture solution, adding 150 μL DMSO, vibrating slightly to dissolve crystallization, determining absorbance value by automatic microplate reader at 490 nm, calculating the inhibition ratio of cell group according to following formula.

Inhibition rate of cell growth (Ig)=(1−the absorbance of administration group/the absorbance of black group)×100%

[0087] Experimental Results:

[0088] Referring to table 1, the comparison between the inhibitory effect of the low dose group, medium dose group and high dose group of tested samples to the growth of human hepatoma cell SMMC-7721 and blank group has dramatic difference. Tested sample group has action of inhibiting the growth of human hepatoma cell SMMC-7721, wherein its inhibitory action is superior to that of control group. The inhibitory action to the growth of human hepatoma cell SMMC-7721 of albumin peptide combination is superior to that of coix seed extract individually.

TABLE-US-00001 TABLE 1 Comparison experiment between albumin peptide combination and coix seed to the effect of inhibiting the growth of human hepatoma cell SMMC-7721 Sample Sample Sample Blank Control low dose medium dose high dose group group group group group Inhibition 0.37 23.2 41.5 54.3 52.6 rate(%)

Example 6 the Inhibition Experiment of Albumin Peptide Combination Solutions of Different Concentrations to Gastric Carcinoma Cell

[0089] Preparing and obtaining tested sample group solutions with concentrations of low dose group 10 mg/L, medium dose group 30 mg/L and high dose group 50 mg/L respectively, according to the albumin peptide combination obtained by the process of Example 3.

[0090] Preparing the coix seed extract solution with concentration of 13.3 mg/L as control group, according to the preparation method of the process of the coix seed extract in Example 3.

[0091] Taking Cell Culture Solution as Blank Group.

[0092] Taking the human gastric carcinoma cell BGC823 in logarithmic growth phase, using culture solution to dilute to prepare cell suspension, adjusting the concentration of cell to be 5×104/mL. Taking 96 wells culture plate, setting blank group, control group and tested sample group, inoculating cell suspension into 96 wells culture plate according to designing scheme, adding cell suspension 200 μL into each well. Culturing 96 wells culture plate in 5% CO2 incubator under 37° C. for 24 h, pouring out supernatant, adding 20 μL albumin peptide combination solutions of different concentrations respectively for tested sample group, then adding culture solution to 200 μL, adding 20 μL coix seed extract solution for control group, then adding culture solution to 200 μL, directly adding culture solution for blank group to sufficient amount, mixing homogenously; setting 6 parallel wells for each group, continue culturing for 24 h, pouring out supernatant, adding 5 mg/mL MTT 20 μL per well, culture solution 180 μL. Centrifuging 96 wells culture plate under condition of 1000 r/min for 10 min, sucking and discarding the supernatant in wells carefully, adding DMSO 100 μL per well, vibrating slightly to dissolve crystallization, determining absorbance value by automatic microplate reader at 490 nm, calculating the inhibition ratio of cell group according to following formula.

Inhibition rate of cell growth (Ig)=(1−the absorbance of administration group/the absorbance of blank group)×100%

[0093] Experimental Results:

[0094] Referring to table 2, the comparison between the inhibitory effect of the low dose group, medium dose group and high dose group of tested samples to the growth of human gastric carcinoma cell BGC823 and blank group has dramatic difference. Tested sample group has action of inhibiting the growth of human gastric carcinoma cell BGC823, wherein its inhibitory action is superior to that of control group. The inhibitory action to the growth of human gastric carcinoma cell BGC823 of albumin peptide combination is superior to that of coix seed extract individually.

TABLE-US-00002 TABLE 2 Comparison experiment between albumin peptide combination and coix seed to the effect of inhibiting the growth of human gastric carcinoma cell BGC823 Sample Sample Sample Blank Control low dose medium dose high dose group group group group group Inhibition 0.41 19.2 46.5 59.1 61.7 rate(%)

Example 7 the Inhibition Experiment of Albumin Peptide Combination Solutions of Different Concentrations to Human Lung Carcinoma Cell SPC-A1

[0095] Preparing and obtaining tested sample group solutions with concentrations of low dose group 10 mg/L, medium dose group 30 mg/L and high dose group 50 mg/L respectively, according to the albumin peptide combination obtained by the process of Example 3.

[0096] Preparing the coix seed extract solution with concentration of 13.3 mg/L as control group, according to the preparation method of the process of the coix seed extract in Example 3.

[0097] Taking Cell Culture Solution as Blank Group.

[0098] Taking the human lung carcinoma cell SPC-A1 in logarithmic growth phase, using culture solution to dilute to prepare cell suspension, adjusting the concentration of cell to be 5× 104/mL. Taking 96 wells culture plate, setting blank group, control group and tested sample group, inoculating cell suspension into 96 wells culture plate according to designing scheme, adding cell suspension 200 μL into each well. Culturing 96 wells culture plate in 5% CO2 incubator under 37° C. for 24 h, pouring out supernatant, adding 20 μL albumin peptide combination solutions of different concentrations respectively for tested sample group, then adding culture solution to 200 μL, adding 20 μL coix seed extract solution for control group, then adding culture solution to 200 μL, directly adding culture solution for blank group to sufficient amount, mixing homogenously; setting 6 parallel wells for each group, continue culturing for 24 h, pouring out supernatant, adding 5 mg/mL MTT 20 μL per well, culture solution 180 μL. Centrifuging 96 wells culture plate under condition of 1000 r/min for 10 min, sucking and discarding the supernatant in wells carefully, adding DMSO 100 μL per well, vibrating slightly to dissolve crystallization, determining absorbance value by automatic microplate reader at 490 nm, calculating the inhibition rate of cell group according to following formula.

Inhibition rate of cell growth (Ig)=(1−the absorbance of administration group/the absorbance of blank group)×100%

[0099] Experimental Results:

[0100] Referring to table 3, the comparison between the inhibitory effect of the low dose group, medium dose group and high dose group of tested samples to the growth of human lung carcinoma cell SPC-A1 and blank group has dramatic difference. Tested sample group has dramatic action of inhibiting the growth of human lung carcinoma cell SPC-A1, wherein its inhibitory action is superior to that of control group. The inhibitory action to the growth of human lung carcinoma cell SPC-A1 of albumin peptide combination is superior to that of coix seed extract individually.

TABLE-US-00003 TABLE 3 Comparison experiment between albumin peptide combination and coix seed to the effect of inhibiting the growth of human lung carcinoma cell SPC-A1 Sample Sample Sample Blank Control low dose medium dose high dose group group group group group Inhibition 0.29 18.1 38.9 51.2 59.3 rate(%)

Example 8 the Inhibition Experiment of Albumin Peptide Combination Solutions of Different Concentrations to Human Cervical Cancer Cell Hela

[0101] Preparing and obtaining tested sample group solutions with concentrations of low dose group 10 mg/L, medium dose group 30 mg/L and high dose group 50 mg/L respectively, according to the albumin peptide combination obtained by the process of Example 3.

[0102] Preparing the coix seed extract solution with concentration being 13.3 mg/L as control group, according to the preparation method of the process of the coix seed extract in Example 3.

[0103] Taking Cell Culture as Blank Group.

[0104] Taking the human cervical cancer cell Hela in logarithmic growth phase, using culture solution to dilute to prepare cell suspension, adjusting the concentration of cell to be 5×104/mL. Taking 96 wells culture plate, setting blank group, control group and tested sample groups, inoculating cell suspension into 96 wells culture plate according to designing scheme, adding cell suspension 200 μL into each well. Culturing 96 wells culture plate in 5% CO2 incubator under 37° C. for 24 h, pouring out supernatant, adding 20 μL albumin peptide combination solutions of different concentrations respectively for tested sample group, then adding culture solution to 200 μL, adding 20 μL coix seed extract solution for control group, then adding culture solution to 200 μL, directly adding culture solution for blank group to sufficient amount, mixing homogenously; setting 6 parallel wells for each group, continue culturing for 72 h, pouring out supernatant, adding 5 mg/mL MTT 20 μL per well, continue culturing for 4 h. Centrifuging 96 wells culture plate under condition of 1000 r/min for 10 min, sucking and discarding the supernatant in wells carefully, adding DMSO 100 μL per well, vibrating slightly to dissolve crystallization, determining absorbance value by automatic microplate reader at 490 nm, calculating the inhibition ratio of cell group according to following formula.

Inhibition rate of cell growth (Ig)=(1−the absorbance of administration group/the absorbance of blank group)×100%

[0105] Experimental Results:

[0106] Referring to table 4, the comparison between the inhibitory effect of the low dose group, medium dose group and high dose group of tested samples to the growth of human cervical cancer cell Hela and blank group has dramatic difference. Tested sample group has action of inhibiting the growth of human cervical cancer cell Hela, wherein its inhibitory action is superior to that of control group. The inhibitory action to the growth of human cervical cancer cell Hela of albumin peptide combination is superior to that of coix seed extract individually.

TABLE-US-00004 TABLE 4 Comparison experiment between albumin peptide combination and coix seed to the effect of inhibiting the growth of human cervical cancer cell Hela Sample Sample Sample Blank Control low dose medium dose high dose group group group group group Inhibition 0.49 23.8 45.8 56.7 63.2 rate(%)

[0107] It shall be stated last that: the above is merely preferably Examples of the present invention, but not used to define the present invention. Though detailed explanation is performed to the present invention with reference to aforethe Examples, as to a person skilled in the art, he still can perform amendment to the technical schemes recorded in aforethe each Example, or perform equivalent substitution to a part of technical features therein. Any amendments, equivalent substitution, improvement and the like made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

[0108] One skilled in the art will understand that the embodiment of the present invention as shown in the drawings and described above is exemplary only and not intended to be limiting.

[0109] It will thus be seen that the objects of the present invention have been fully and effectively accomplished. The embodiments have been shown and described for the purposes of illustrating the functional and structural principles of the present invention and is subject to change without departure from such principles. Therefore, this invention includes all modifications encompassed within the spirit and scope of the following claims.