COMPOUNDS THAT PARTICIPATE IN COOPERATIVE BINDING AND USES THEREOF

Abstract

The disclosure features macrocyclic compounds, alone and in combination with other therapeutic agents, as well as pharmaceutical compositions and protein complexes thereof, capable of modulating biological processes including RAS and RAS-RAF inhibition, and their uses in the treatment of cancers.

Claims

1. A compound of formula I: ##STR00525## a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein: Q is a bicyclic arylene, a bicyclic heteroarylene, or a bicyclic heterocyclylene, wherein a first ring in Q is bonded to X, and a second ring in Q is bonded to Z, and wherein Q is optionally substituted; X is a bond; a straight chain C.sub.1-C.sub.3 alkylene optionally substituted with 1 to 3 substituents independently selected from fluoro, CN, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; O; S(O).sub.0-2; *CH.sub.2O; *CH.sub.2S(O).sub.0-2; *OCH.sub.2; or *CH.sub.2S(O).sub.0-2, wherein * represents a portion of X bound to C(R.sup.4)(R.sup.5); Y is O, NH or N(C.sub.1-C.sub.3 alkyl)-; ring Z is phenyl or a 6-membered heteroaryl; R.sup.1 is optionally substituted C.sub.1-C.sub.6 alkyl, (CH.sub.2).sub.0-1(C.sub.3-C.sub.6 optionally substituted cycloalkyl), (CH.sub.2).sub.0-1-(optionally substituted aryl), or optionally substituted heterocyclyl; R.sup.2 is: ##STR00526## wherein: ring A is a 4-8 membered cycloalkyl or a 4-8 membered heterocyclyl; W is N(R.sup.12), O, or C(R.sup.12a)(R.sup.12b); each R.sup.A is each independently fluoro; chloro; CN; OH; NH.sub.2; C.sub.1-C.sub.3 alkyl optionally substituted with CN, OH, NH.sub.2 or OC.sub.1-C.sub.3 alkyl; OC.sub.1-C.sub.3 alkyl; or NHC.sub.1-C.sub.3 alkyl; R.sup.9, if present, is N(C.sub.0-C.sub.5 alkylene-H), N(C(O)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C.sub.0-C.sub.5 alkylene-H), or C(C.sub.0-C.sub.3 alkylene-H)(C(O)C.sub.0-C.sub.5 alkylene-H), wherein each alkylene portion of R.sup.9 is optionally substituted with one or more substituent, wherein each substituent is, independently, selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; R.sup.10, if present, is C.sub.1-C.sub.4 alkylene optionally substituted with one or more substituent, wherein each substituent is, independently, selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; R.sup.11 is N(C.sub.0-C.sub.5 alkylene-H), N(C(O)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C(O)C.sub.0-C.sub.5 alkylene-H), or a saturated, nitrogen-containing heterocyclyl, where each alkylene portion of R.sup.11 is optionally substituted with one or more substituent, wherein each substituent is, independently, selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; R.sup.12 is hydrogen, or C.sub.1-C.sub.3 alkyl, or R.sup.12 is taken together with one R.sup.A, the atoms to which they are respectively attached and any intervening atoms to form an optionally substituted, 5-8 membered heterocyclyl that is fused or spiro-fused to ring A, or R.sup.12 is taken together with any methylene unit in R.sup.10, or any methylene unit in R.sup.11, the atoms to which they are respectively attached and any intervening atoms to form an optionally substituted, 5-8 membered heterocyclyl; each of R.sup.12a and R.sup.12b are independently hydrogen, or C.sub.1-C.sub.3 alkyl, or R.sup.12a and R.sup.12b are taken together with the carbon atom to which they are bound to form a 3-6 membered cycloalkyl ring; R.sup.13 is O, S, NCN, or NOC.sub.1-C.sub.3 alkyl; and WH is ##STR00527## each R.sup.14 is independently hydrogen, CN, or C.sub.1-C.sub.3 alkyl optionally substituted with one or more substituents independently selected from OH, OC.sub.1-C.sub.3 alkyl, NH.sub.2, NH(C.sub.1-C.sub.3 alkyl), N(C.sub.1-C.sub.3 alkyl).sub.2, or an optionally substituted 4-7 membered saturated heterocyclyl; R.sup.15 is C.sub.1-C.sub.3 alkyl optionally substituted with one or more substituents independently selected from OH, OC.sub.1-C.sub.3 alkyl, NH.sub.2, NH(C.sub.1-C.sub.3 alkyl), N(C.sub.1-C.sub.3 alkyl).sub.2, or an optionally substituted 4-7 membered saturated heterocyclyl; R.sup.16 is hydrogen, C.sub.1-C.sub.3 alkyl optionally substituted with one or more substituents independently selected from OH, OC.sub.1-C.sub.3 alkyl, NH.sub.2, NH(C.sub.1-C.sub.3 alkyl), N(C.sub.1-C.sub.3 alkyl).sub.2, or an optionally substituted 4-7 membered saturated heterocyclyl; or R.sup.14 is taken together with either of R.sup.9 or R.sup.11, the atoms to which they are attached and any intervening atoms to form an optionally substituted 5-8 membered ring system; or R.sup.11 is taken together with either of R.sup.9 or R.sup.11, the atoms to which they are attached and any intervening atoms to form an optionally substituted 5-8 membered ring system; R.sup.3 is hydrogen, halogen, C.sub.1-C.sub.3 alkyl, or C.sub.1-C.sub.3 hydroxyalkyl; R.sup.4 is hydrogen, halogen, or optionally substituted C.sub.1-C.sub.3 alkyl; R.sup.5 is hydrogen, halogen, OH, CN, O-(optionally substituted C.sub.1-C.sub.3 alkyl), optionally substituted C.sub.1-C.sub.3 alkyl, optionally substituted C.sub.2-C.sub.6 alkenyl, optionally substituted C.sub.2-C.sub.6 alkynyl, (CH.sub.2).sub.0-1-aryl, (CH.sub.2).sub.0-1-heteroaryl, (CH.sub.2).sub.0-1-cycloalkyl, or (CH.sub.2).sub.0-1-heterocyclyl; or R.sup.4 and R.sup.5 are taken together to form CH.sub.2, an optionally substituted C.sub.3-C.sub.6 cycloalkyl, or a 3-7 membered saturated heterocyclyl; or R.sup.5 is taken together with a ring atom in Q, the carbon atom to which R.sup.4 is bound and X to form a 4-9 membered saturated or unsaturated heterocyclyl that is fused to Q; R.sup.6 is hydrogen or CH.sub.3; each R.sup.7 is independently halo, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 hydroxyalkyl, OH, OC.sub.1-C.sub.3 alkyl, OC.sub.1-C.sub.3 haloalkyl, NR.sup.n1R.sup.n2, NR.sup.n1OR.sup.n2, ONR.sup.n1R.sup.n2, or NR.sup.n1NR.sup.n2R.sup.n3; R.sup.n1 is H, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 heteroalkyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 aminoalkyl, wherein one methylene unit of R.sup.n1 is optionally substituted with ##STR00528## R.sup.n2 is H, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 heteroalkyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 aminoalkyl, wherein one methylene unit of R.sup.n2 is optionally substituted with ##STR00529## R.sup.n3 is H, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 heteroalkyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 aminoalkyl, wherein one methylene unit of R.sup.n3 is optionally substituted with ##STR00530## each R.sup.6 is independently halo, C.sub.1-C.sub.3 alkyl, or C.sub.1-C.sub.3 haloalkyl; n is 0, 1, 2, 3, 4, 5, or 6; p is 0, 1, 2, or 3; and r is 0, 1, 2, 3, or 4.

2. The compound of claim 1, wherein said compound has the structure of formula (Ia): ##STR00531## or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein: X is a bond, O, CH.sub.2, CH(CH.sub.3), *CH.sub.2O, or CH.sub.2CH.sub.2, wherein * represents a portion of X bound to C(R.sup.4)(R.sup.5); Y is O or NH; R.sup.1 is C.sub.1-C.sub.4 alkyl, (CH.sub.2).sub.0-1(C.sub.3-C.sub.6 cycloalkyl), or C.sub.4-C.sub.6 cycloalkyl; R.sup.2 is: ##STR00532## wherein: ring A is a 4-8 membered cycloalkyl or a 4-8 membered saturated heterocyclyl; each R.sup.A is each independently fluoro; chloro; CN; OH; NH.sub.2; C.sub.1-C.sub.3 alkyl optionally substituted with CN, OH, NH.sub.2 or OC.sub.1-C.sub.3 alkyl; OC.sub.1-C.sub.3 alkyl; or NHC.sub.1-C.sub.3 alkyl; n is 0, 1, 2, 3, 4, 5, or 6; R.sup.9, if present, is N(C.sub.0-C.sub.5 alkylene-H), N(C(O)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C.sub.0-C.sub.5 alkylene-H), or C(C.sub.0-C.sub.3 alkylene-H)(C(O)C.sub.0-C.sub.5 alkylene-H), wherein each alkylene portion of R.sup.9 is optionally substituted with one or more substituent independently selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; R.sup.10, if present, is C.sub.1-C.sub.4 alkylene optionally substituted with one or more substituent independently selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; R.sup.11 is N(C.sub.0-C.sub.5 alkylene-H), N(C(O)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C.sub.0-C.sub.5 alkylene-H), or C(C.sub.0-C.sub.3 alkylene-H)(C(O)C.sub.0-C.sub.5 alkylene-H), wherein each alkylene portion of R.sup.11 is optionally substituted with one or more substituent independently selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; R.sup.12 is hydrogen, or C.sub.1-C.sub.3 alkyl, or R.sup.12 is taken together with one R.sup.A, the atoms to which they are respectively attached and any intervening atoms to form an optionally substituted, 5-8 membered heterocyclyl that is fused to ring A, or R.sup.12 is taken together with any methylene unit in R.sup.10, or any methylene unit in R.sup.11, the atoms to which they are respectively attached and any intervening atoms to form an optionally substituted, 5-8 membered heterocyclyl; WH is ##STR00533## each R.sup.14 is independently hydrogen, CN, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 hydroxyalkyl, OC.sub.1-C.sub.3 alkyl; R.sup.15 is C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 alkylene-OC.sub.1-C.sub.3 alkyl; R.sup.16 is hydrogen, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 alkylene-OC.sub.1-C.sub.3 alkyl; or R.sup.14 is taken together with either of R.sup.9 or R.sup.11, the atoms to which they are attached and any intervening atoms to form an optionally substituted 5-8 membered ring system, or R.sup.16 is taken together with either of R.sup.9 or R.sup.11, the atoms to which they are attached and any intervening atoms to form an optionally substituted 5-8 membered ring system; R.sup.4 is hydrogen, halo, or C.sub.1-C.sub.3 alkyl; R.sup.5 is hydrogen, halo, OH, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 hydroxyalkyl, C.sub.1-C.sub.3 alkylene-OC.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 haloalkyl, (CH.sub.2).sub.0-1C.sub.3-C.sub.6 cycloalkyl, C.sub.1-C.sub.3 cyanoalkyl, or (CH.sub.2).sub.0-1-aryl (benzyl), or R.sup.4 and R.sup.5 are taken together to form CH.sub.2, or a C.sub.3-C.sub.6 cycloalkyl, or R.sup.5 is taken together with a ring atom of Q, the carbon atom to which it is bound and X to form a 5-7 membered saturated heterocyclyl; R.sup.7 is OH, NH.sub.2, or C.sub.1-C.sub.3 haloalkyl; Q is a bicyclic arylene, a bicyclic heteroarylene, or a bicyclic heterocyclylene, wherein: a first ring in Q is bonded to X, and a second ring in Q is bonded Z; and Q is optionally substituted with one or more independently selected substituents selected from O; CN; C.sub.1-C.sub.5 alkyl optionally substituted with one or more independently selected halo, CN, OH, O(C.sub.1-C.sub.3 alkyl), C(O)(C.sub.1-C.sub.3 alkyl), O(C.sub.2-C.sub.3 alkynyl), (C.sub.3-C.sub.6 cycloalkyl), or a 4-7 membered saturated heterocyclyl; O(C.sub.1-C.sub.3 alkyl) optionally substituted with one or more independently selected halo; C.sub.2-C.sub.5 alkenyl optionally substituted with one or more independently selected CN, or OH; C.sub.2-C.sub.3 alkynyl; S(O).sub.2C.sub.1-C.sub.3 alkyl; (CH.sub.2).sub.0-1C.sub.3-C.sub.6 cycloalkyl optionally substituted with one or more independently selected halo, O, CN, C.sub.1-C.sub.3 alkyl optionally substituted with CN or OC.sub.1-C.sub.3 alkyl, C(O)-saturated heterocyclyl, O-saturated heterocyclyl, O-cycloalkyl, or O-aryl; (CH.sub.2).sub.0-1-heteroaryl optionally substituted with one or more independently selected halo, CN, C.sub.1-C.sub.3 alkyl optionally substituted with CN or OC.sub.1-C.sub.3 alkyl, C(O)-saturated heterocyclyl, O-saturated heterocyclyl, O-cycloalkyl, or O-aryl; (CH.sub.2).sub.0-1-heterocyclyl optionally substituted with one or more independently selected halo, O, CN, C.sub.1-C.sub.3 alkyl optionally substituted with CN or OC.sub.1-C.sub.3 alkyl, C(O)-saturated heterocyclyl, O-saturated heterocyclyl, O-cycloalkyl, or O-aryl; (CH.sub.2).sub.0-1-aryl optionally substituted with one or more independently selected halo, CN, C.sub.1-C.sub.3 alkyl optionally substituted with CN or OC.sub.1-C.sub.3 alkyl, C(O)-saturated heterocyclyl, O-saturated heterocyclyl, O-cycloalkyl, or O-aryl; C(O)NH(C.sub.1-C.sub.3 alkyl); C(O)N(C.sub.1-C.sub.3 alkyl).sub.2; C.sub.2-C.sub.3 alkenylene NO(C.sub.1-C.sub.3 alkyl) optionally substituted with C.sub.3-C.sub.6 cycloalkyl; or two substituents on the same or adjacent ring atoms of Q are taken together to form a 5-7 membered monocyclic ring or a 6-12 membered bicyclic ring optionally substituted with one or more independently selected halo, O, CN, C.sub.1-C.sub.3 alkyl, or OC.sub.1-C.sub.3 alkyl; and fused to Q.

3. The compound of claim 2, wherein said compound has the structure of formula (Ib): ##STR00534## or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof or said compound has the structure of formula (Ic): ##STR00535## or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

4. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is a 5,6 bicyclic heteroarylene, a 5,6 bicyclic heterocyclylene, a 6,6 bicyclic heteroarylene, or a 6,6 bicyclic heterocyclylene; and wherein Q is optionally substituted.

5. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of: ##STR00536## wherein: each of V.sub.1, V.sub.2, V.sub.3 and V.sub.4 is independently C, CH, or N; R.sup.Q1 is S(O).sub.2R.sup.Q11, C(O)R.sup.Q11, S(O).sub.2N(R.sup.Q11)R.sup.Q12, C(O)N(R.sup.Q11)R.sup.Q12, C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl are optionally substituted; or R.sup.Q1 is taken together with the nitrogen atom to which it is attached and an adjacent ring atom to form an optionally substituted 4-8 membered ring, which is optionally further fused to a 5-6 membered ring; each of R.sup.Q11 and R.sup.Q12 is independently C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein each of R.sup.Q11 and R.sup.Q12 is optionally substituted; or R.sup.Q11 and R.sup.Q12 are taken together with the nitrogen atom to which they are both attached to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking R.sup.Q11 and R.sup.Q12 together is optionally fused to another 5-6 membered ring.

6. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of: ##STR00537## wherein: each of V.sub.1, V.sub.2, V.sub.3 and V.sub.4 is independently C, CH, N, C(F), C(CH.sub.3), C(OH), C(OCH.sub.3), or C(CN); each of V.sub.5, V.sub.6, and V.sub.7 is independently, C(R.sup.17a)(R.sup.17b), or C(O), wherein each of R.sup.17a and R.sup.17b is independently selected from hydrogen, halo, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 haloalkyl, OC.sub.1-C.sub.3 alkyl, OC.sub.1-C.sub.3 haloalkyl, and no more than two of V.sub.5, V.sub.6, and V.sub.7 is C(O); R.sup.NQ1 is hydrogen, optionally substituted S(O).sub.2R.sup.Q11, C(O)R.sup.Q11, S(O).sub.2N(R.sup.Q11)R.sup.Q12, C(O)N(R.sup.Q11)R.sup.Q12, C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl are optionally substituted; each R.sup.Q2 is independently hydrogen, CN, optionally substituted S(O).sub.2R.sup.Q11, C(O)R.sup.Q11, S(O).sub.2N(R.sup.Q11)R.sup.Q12, C(O)N(R.sup.Q11)R.sup.Q12, C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl are optionally substituted; or R.sup.NQ1 and one R.sup.Q2 are taken together with the atoms to which they are bound to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking R.sup.NQ1 and one R.sup.Q2 together is optionally further fused to a 5-6 membered ring; each R.sup.Q3 is independently hydrogen, CN, optionally substituted S(O).sub.2R.sup.Q11, C(O)R.sup.Q11, S(O).sub.2N(R.sup.Q11)R.sup.Q12, C(O)N(R.sup.Q11)R.sup.Q12, C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl are optionally substituted, or two R.sup.Q3 bound to the same atom are taken together to form CH, O, S, or NR.sup.V4; or two R.sup.Q3 bound to the same atom are taken together with the atom to which they are bound to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking each R.sup.Q3 together is optionally further fused to a 5-6 membered ring; or R.sup.NQ1 and one R.sup.Q3 are taken together with the atoms to which they are bound to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking R.sup.NQ1 and R.sup.Q3 together is optionally further fused to a 5-6 membered ring; each of R.sup.Q11 and R.sup.Q12 is independently C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein each of R.sup.Q11 and R.sup.Q12 is optionally substituted; or R.sup.Q11 and R.sup.Q12 are taken together with the atoms to which they are attached to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking R.sup.Q11 and R.sup.Q12 together is optionally fused to another 5-6 membered ring; and * represents a portion of Q that is bound to ring Z.

7. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of: ##STR00538##

8. The compound of claim 7, wherein said compound has the structure of formula (Id): ##STR00539## or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof or said compound has the structure of formula (Ij): ##STR00540## a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

9. The compound of claim 1, wherein said compound has the structure of formula (IL): ##STR00541## or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.18 is Br or Cl or said compound has the structure of formula (Im): ##STR00542## or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein each R.sup.14 is H.

10. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of: ##STR00543## ##STR00544## wherein: 1 indicates a portion of Q bound to X; and Q is further optionally substituted.

11. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of: ##STR00545## wherein: R is CH.sub.2CH.sub.3, CH.sub.2CH.sub.2OCH.sub.3, CH.sub.2CHF.sub.2, CH.sub.2CN, C(CH.sub.3).sub.2CN, C(CH.sub.3).sub.2CH.sub.2CN, CH.sub.2CH.sub.2CN, cyclohexyl, cyclobutyl, cyclopropyl, pyridin-4-yl, tetrahydropyran-4-yl, tetrahydropyran-4-ylmethyl, oxetan-3-ylmethyl, 2-cyano-5-methoxyphenyl, 2-cyano-5-methoxymethylphenyl, 2-cyano-6-(methoxymethyl)phenyl, 2-cyano-6-bromophenyl, 2-methoxyethan-1-yl, 2-cyanopropan-2-yl, 2-tetrahydropyran-4-ylethan-1-yl, 3-cyanopentan-3-yl, or 2-cyano-4-methoxybut an-2-yl, or R is ##STR00546## R.sup.23 is hydrogen or fluoro; R.sup.24 is hydrogen, chloro, CN, CH.sub.3, CH.sub.2CH.sub.3, CHF.sub.2, CF.sub.3, CH.sub.2CN, CH(CN)CH.sub.3, C(CH.sub.3).sub.2CN, C(CH.sub.2CH.sub.3).sub.2CN, CH.sub.2CH.sub.2CN, C(CH.sub.3)NOCH(CH.sub.3).sub.2, C(CH.sub.3)NOCH.sub.3, C(O)N(CH.sub.3).sub.2, C(O)NHCH.sub.3, OCH.sub.3, CH.sub.2OCH.sub.3, CCH, CCCH.sub.3, S(O).sub.2CH.sub.3, 1-(cyclopentyl)-1-cyanoethan-1-yl, 1-(tetrahydropyran-4-yl)-1-cyanoethan-1-yl, 1-(tetrahydrofuran-3-yl)-1-cyanoethan-1-yl, 1,3-dimethoxy-2-cyanopropan-2-yl, 1,4-dimethylpyrazol-5-yl, 1-cyanocyclobutyl, 1-cyanocyclopropyl, 1-cyanocylopentyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, 1-methylpyrazol-3-yl, 1-methylpyrazol-4-ylcyanomethyl, 1-methylpiperidin-4-yl, 1-methylpyrazol-5-yl, 1-oxoindolin-5-yl, 1-oxoisoindolin-4-yl, 1-oxoisoindolin-6-yl, 2-(2-methoxyethan-1-yl)phenyl, 2-(methoxymethyl)phenyl, 2-(tetrahydropyran-4-yloxy)phenyl, 2,2-difluoro-benzo[d][1,3]dioxol-4-yl, 2,3-dicyanopropan-2-yl, 2-chiorophenyl, 2-cyano-3-(tetrahydropyran-4-yl)propan-2-yl, 2-cyano-3-chlorophenyl, 2-cyano-3-fluorophenyl, 2-cyano-3-methoxyphenyl, 2-cyano-4-fluorophenyl, 2-cyano-4-chlorophenyl, 2-cyano-5-chlorophenyl, 2-cyano-5-fluorophenyl, 2-cyano-5-methoxyphenyl, 2-cyano-6-chlorophenyl, 2-cyano-6-fluorophenyl, 2-cyano-6-(tetrahydropyran-4-yloxy)phenyl, 2-cyanomethylphenyl, 2-cyanophenyl, 2-cyanopropan-2-yl, 2-cyclopentylphenyl, 2-difluoromethoxyphenyl, 2-fluorophenyl, 2-methoxy-6-cyanophenyl, 2-methoxyphenyl, 2-methoxycarbonylphenyl, 2-nitrophenyl, 2-oxopyrrolidin-1-yl, 2-phenoxyphenyl, 3-(1,1-dioxothiomorpholin-4-ylmethyl)phenyl, 3-(2-methoxyethan-1-yl)phenyl, 3,5-difluoro-4-(pyrrolidin-1-ylcarbonyl)phenyl, 3-cyano-2-methylpropan-2-yl, 3-cyanomethylphenyl, 3-cyanopentan-3-yl, 3-cyanophenyl, 3-hydroxy-2-methylbutan-2-yl, 3-hydroxy-3-methyl-but-1-yne-1-yl, 3-methoxy-2-methylbutan-2-yl, 3-methoxymethyl-5-methylisoxazol-4-yl, 3-methoxyphenyl, 3-methoxycarbonylphenyl, 3-oxo-2-methylbutan-2-yl, 4-cyanophenyl, 4-cyanotetrahydropyran-4-yl, 4-methoxyphenyl, benzo[d][1,3]dioxol-4-yl, benzo[d]oxazol-7-yl, benzo[d]thiazol-2-yl, benzo[d]thiazol-4-yl, benzo[d]thiazol-5-yl, benzo[d]thiazol-6-yl, benzo[d]thiazol-7-yl, cyclobutyl, cyclopropyl, cyclopropylcyanomethyl, N-methoxycyclopropanecarbimidoyl, phenyl, pyridin-2-ylmethyl, pyridin-3-yl, pyridin-3-ylmethyl, pyridin-4-ylmethyl, tetrahydrofuran-3-ylmethyl, tetrahydrofuran-3-ylcyanomethyl, tetrahydropyran-4-yl, or tetrahydropyran-4-ylcyanomethyl; R.sup.27 is hydrogen, CH.sub.3, CHF.sub.2, CH.sub.2CH.sub.3, CH.sub.2OCH.sub.3, CH.sub.2CN, CN, CH.sub.2OCH.sub.2CN, C(O)N(CH.sub.3).sub.2, C(O)NHCH.sub.3, CH.sub.2OCH.sub.2CCH, 2-methoxyphenyl, 3-methoxyphenyl, 2,2-difluorobenzo[d][1,3]dioxol-4-yl, 2-cyanophenyl, 3-cyanophenyl, phenyl, 2-benzyl methyl ether, 2-(2-methoxyethyl) benzene, 2-(2-difluoromethoxymethyl)benzene, 2-(2-dimethylmethoxyethyl)benzene, pyridin-3-yl, pyridin-2-yl, pyridin-3-ylmethyl, or tetrahydropyridin-4-yl, or R.sup.24 and R.sup.27 are taken together to form 4-cyanobenzene-1,2-diyl, 3-cyanobenzene-1,2-diyl, 5-methyl-5-cyanotetrahydropyran-3,4-diyl, 3-cyanocyclohexan-1,2-diyl, 3-methoxybenzene-1,2-diyl, benzene-1,2-diyl, 3-oxocyclohexyl-1,2-diyl, 3-cyanocyclopentan-1,2-diyl, or pyridin-3,4-diyl; R.sup.28 is hydrogen, CH.sub.3, or CH.sub.2OCH.sub.3; and R.sup.29 is hydrogen, acetyl, CN, CH.sub.2CN, CH.sub.2CH.sub.2CN, CH.sub.2OCH.sub.3, CHCHCN, CH.sub.2OC(O)N(CH.sub.3).sub.2, morpholin-4-ylmethyl, pyrazol-1-ylmethyl, pyridin-3-yl, pyridin-3-ylethynyl, pyridin-2-yloxymethyl, or 2-cyanopropan-2-yl, or R.sup.28 and R.sup.29 are taken together to form 2,3-dihydrobenzofuran-3,3-diyl, 2,3-dihydrofuro[2,3-b]pyridin-3,3-diyl, tetrahydropyran-3,3-diyl, 6,7-dihydro-5H-cyclopenta[c]pyridin-6-yl, tetrahydropyran-4,4-diyl, or 4-methoxycyclohexane.

12. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.1 is CH.sub.3, CH.sub.2CH.sub.3, (CH.sub.2).sub.2CH.sub.3, CH(CH.sub.3).sub.2, CH(CH.sub.3)CH.sub.2CH.sub.3, cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl, cyclohexylmethyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, phenyl, 4-methoxybenzyl, or tetrahydropyran-4-yl.

13. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.9 is absent and ring A is a saturated, nitrogen-containing heterocyclyl.

14. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 represented by: ##STR00547## is selected from the group consisting of: ##STR00548## ##STR00549## wherein each ring system in R.sup.2 is optionally substituted with up to 4 substituents independently selected from fluoro; chloro; CN; OH; NH.sub.2; C.sub.1-C.sub.3 alkyl optionally substituted with CN, OH, NH.sub.2 or OC.sub.1-C.sub.3 alkyl; OC.sub.1-C.sub.3 alkyl; and NHC.sub.1-C.sub.3 alkyl.

15. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein: the portion of R.sup.2 represented by WH is C(O)CCCH.sub.3, C(O)CHCH.sub.2, S(O).sub.2CHCH.sub.2, C(O)CH.sub.2Cl, C(O)CH(CH.sub.3)Cl, or C(O)CH(Cl)CH.sub.2OCH.sub.3, or the portion of R.sup.2 represented by R.sup.11WH, when R.sup.11 is taken together with one R.sup.14 is ##STR00550##

16. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.2 is selected from the group consisting of: 1-(2-chloro-3-methoxypropanoyl)azetidin-3-yl-N-methylcarboxamido, 1-(2-chloroacetyl)azetidin-3-ylcarboxamido, 1-(2-chloroacetyl)azetidin-3-yl-N-ethylcarboxamido, 1-(2-chloroacetyl)azetidin-3-yl-N-methylcarboxamido, 1-(2-chloroacetyl) piperidin-3-yl-N-methylcarboxamido, 1-(2-chloroacetyl)piperidin-4-yl-N-methylcarboxamido, 1-(2-chloroacetyl) pyrrolidin-3-yl-N-methylcarboxamido, 1-(2-chloropropanoyl)-piperidin-4-yl-N-methylcarboxamido 1-(2-chloropropanoyl)-3-fluoroazetidin-3-yl-N-methylcarboxamido 1-(2-chloropropanoyl)azetidin-3-yl-N-methylcarboxamido, 1-(2-chloropropanoyl)pyrolidin-3-yl-N-methylcarboxamido, 1-(but-2-ynoyl)-4-fluoropiperidin-4-ylcarbonylmethylamino, 1-(but-2-ynoyl))azetidin-2-yl-N-methylcarboxamido, 1-(but-2-ynoyl)azetidin-3-yl-N-methylcarboxamido, 1-(but-2-ynoyl)-piperidin-3-ylcarbonylmethylamino, 1-(but-2-ynoyl-piperidin-4-ylcarbonylmethylamino, 1-(but-2-ynoyl)pyrrolidin-2-ylcarbonyl-N-methylamino 1-(but-2-ynoyl)pyrrolidin-3-ylcarbonyl-N-methylamino 1-acryloyl-2-oxo-imidazolidin-3-yl, 1-acryloyl-3-fluoroazetidin-3-yl-N-methylcarboxamido, 1-acryloyl-3-fluoropyrrolidin-3-yl-N-methylcarboxamido, 1-acryloyl-4-fluoropiperidin-4-ylcarbonylmethylamino 1-acryloylazetidin-2-yl-N-methylcarboxamido, 1-acryloylazetidin-3-yl-N-methylcarboxamido, 1-acryloyl-piperidin-3-ylcarbonylmethylamino, 1-acryloyl-piperidin-4-ylcarbonylmethylamino, 1-acryloylpyrrolidin-2-yl-N-methylcarboxamido, 1-acryloylpyrrolidin-3-yl-N-methylcarboxamido, 1-oxo-7-(2-chloroacetyl)-2,7-diazaspiro[4.3]octan-2-yl, 1-oxo-7-(2-chloroacetyl)-2,7-diazaspiro[4.4]nonan-2-yl, 1-oxo-2-(2-chloroacetyl)-2,7-diazaspiro[4.5]decan-7-yl, 1-oxo-7-(2-chloroacetyl)-2,7-diazaspiro[4.5]decan-2-yl, 1-oxo-7-(2-chloropropanoyl)-2,7-diazaspiro[4.3]octan-2-yl, 1-oxo-7-(but-2-ynoyl)-2,7-diazaspiro[4.4]nonan-2-yl, 1-oxo-7-acryloyl-2,7-diazaspiro[4.3]octan-2-yl, 1-oxo-7-acryloyl-2,7-diazaspiro[4.4]nonan-2-yl, 1-oxo-7-acryloyl-2,7-diazaspiro[4.5]decan-2-yl, 1-oxo-8-(2-chloroacetyl)-2,8-diazaspiro[4.5]decan-2-yl, 1-oxo-8-(but-2-ynoyl)-2,8-diazaspiro[4.5]decan-2-yl, 1-oxo-8-acryloyl-2,8-diazaspiro[4.5]decan-2-yl, 1-vinylsulfonyl-2-oxoimidazolidin-3-yl, 1-vinylsulfonylazetidin-3-N-methylcarboxamido, 2-(1-acryloylpiperidin-4-yl)-N-methylacetamido, 2-(but-2-ynoyl)-5-oxo-2,6-diazaspiro[3.4]octan-1-yl, 2,5-dioxo-3,4-dimethyl-2,5-dihydropyrrol-1-yl-N-methylacetamido, 2-acryloyl-2-azabicyclo[2.1.1]hexan-4-yl-N-methylcarboxamido, 2-chloroacetamidomethyl-N-methylcarboxamido, 2-oxo-2,5-dihydro-1H-pyrrol-1-yl-N-methylacetamido, 2-oxo-3-(2-chloroacetamido)pyrrolidin-1-yl, 2-oxo-3-(N-methyl-2-chloroacetamido)pyrrolidin-1-yl, 2-oxo-3-(N-methylacrylamido)pyrrolidin-1-yl, 2-oxo-3-acrylamidopyrrolidin-1-yl, 2-oxo-4-(2-chloroacetyl)piperazin-1-yl, 2-oxo-4-acryloylpiperazin-1-yl, 2-oxo-4-vinylsulfonylpiperazin-1-yl, 2-oxocyclopent-3-en-1-yl-N-methylacetamido, 3-(4-(dimethylamino)but-2-enamido)phenyl-N-methylcarboxamido, 4-(but-2-ynoyl)-piperazin-1-yl-N-methylcarboxamido, 4-acryloylpiperazin-1-yl-N-methylcarboxamido, 6-oxo-2-(2-chloroacetyl)-2,7-diazaspiro[4.5]decan-7-yl, and 6-oxo-2-acryloyl-2,7-diazaspiro[4.5]decan-7-y.

17. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein: R.sup.4 is hydrogen, fluoro, or CH.sub.3; and R.sup.5 is hydrogen, fluoro, chloro, OH, CH.sub.3, CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, CH.sub.2OH, CH.sub.2OCH.sub.3, CH.sub.2F, CHF.sub.2, CH.sub.2CN, CH.sub.2-cyclopropyl, cyclopropyl, pyridyl, phenyl, or CH.sub.2-phenyl, wherein any phenyl portion of R.sup.5 is optionally substituted with up to 4 substituents independently selected from halo, CN, and OC.sub.1-C.sub.3 alkyl: or R.sup.4 and R.sup.5 are taken together to form CH.sub.2 or cyclopropyl, or cyclobutyl, or cyclopentyl, or cyclohexyl; or R.sup.5 is taken together with the carbon atom to which it is bound, a ring atom of Q, and X to form oxazepane.

18. The compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.7 is OH, NH.sub.2, or CHF.sub.2.

19. A compound, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, selected from FIG. 1.

20. A pharmaceutical composition comprising a compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, and a pharmaceutically acceptable carrier.

21. A complex comprising a presenter protein, a RAS protein, and a compound of claim 1, or a pharmaceutically acceptable salt thereof.

22. A method of producing a complex, the method comprising contacting a presenter protein and a KRAS G12C protein with a compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, under conditions suitable to permit complex formation.

23. A method of treating cancer in a subject in need thereof, the method comprising administering to the subject an effective amount of a compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

24. A method of inhibiting a KRAS G12C protein in a cell, the method comprising contacting the cell with an effective amount of a compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

25. A method of treating a KRAS G12C protein-related disorder in a subject in need thereof, the method comprising administering to the subject an effective amount of a compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

26. A method of inhibiting RAF-RAS binding in a cell, the method comprising contacting the cell with an effective amount of a compound of claim 1, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

27. The method of claim 23, wherein the method or use further comprises administering an additional therapeutic agent.

28. The method of claim 27, wherein the additional therapeutic agent is a HER2 inhibitor, an EGFR inhibitor, a second Ras inhibitor, a SHP2 inhibitor, a SOS1 inhibitor, a Raf inhibitor, a MEK inhibitor, an ERK inhibitor, a PI3K inhibitor, a PTEN inhibitor, an AKT inhibitor, an mTORC1 inhibitor, a BRAF inhibitor, a PD-L1 inhibitor, a PD-1 inhibitor, a CDK 4/6 inhibitor, or a combination thereof.

Description

BRIEF DESCRIPTION OF THE FIGURES

[0254] FIG. 1 illustrates compounds 1-418 of the invention, the general scheme by which they were made or the specific example that describes their synthesis, and their mass spectrometry and/or NMR values. Compounds 419-461 in FIG. 1 are additional compounds of the invention, prepared using similar methodologies.

DETAILED DESCRIPTION

Compounds

[0255] The present disclosure features compounds of formula (I):

##STR00136##

a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

[0256] The present disclosure features complexes including a presenter protein, a compound of the present invention (e.g., a compound of formula (I) or any of compounds 1-461), or a pharmaceutically acceptable salt, enantiomer, stereoisomer, or tautomer thereof, and a target protein.

[0257] The present disclosure features compounds (e.g., a compound of formula (I) or any of compounds 1-461) capable of modulating biological processes, for example through binding to a presenter protein (e.g., a member of the cyclophilin family) and a target protein (e.g. a member of the RAS family). In some embodiments, the target and/or presenter proteins are intracellular proteins. In some embodiments, the target and/or presenter proteins are mammalian proteins. In some embodiments, provided compounds participate in ternary presenter protein-compound-target protein complexes inside cells, e.g., mammalian cells. In some embodiments, provided compounds may be useful in the treatment of diseases and disorders such as cancer, inflammation, or infections.

Compound Synthesis

[0258] The following general reaction schemes illustrate exemplary methods of making compounds of Formula I, or a pharmaceutically acceptable salt thereof.

[0259] Coupling agents useful in these schemes include, but are not limited to dicyclohexylcarbodiimide (DCC), diisopropylcarbodiimide (DIC), ethyl-(N,N-dimethylamino)propylcarbodiimide hydrochloride (EDC), 1-hydroxybenzotriazole (HOBt)/EDC, (benzotriazol-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate (BOP), (benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate (PyBOP), bromotripyrrolidinophosphonium hexafluorophosphate (PyBROP), (7-azabenzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate (PyAOP), O-(benzotriazol-1-yl)-N, N,NN-tetramethyluronium hexafluorophosphate (HBTU), O-(benzotriazol-1-yl)-N,N,N,N-tetramethyluronium tetrafluoroborate (TBTU), O-(7-azabenzotriazol-1-yl)-N,N,N,N-tetramethyluronium hexafluorophosphate (HATU), O-(7-azabenzotriazol-1-yl)-N,N,N,N-tetramethyluronium tetrafluoroborate (TATU), O-(6-chlorobenzotriazol-1-yl)-N,N,N,N-tetramethyluronium hexafluorophosphate(HCTU), carbonyldiimidazole (CDI), (1-cyano-2-ethoxy-2-oxoethylidenaminooxy)dimethylamino-morpholino-carbenium hexafluorophosphate (COMU), 1-propanephosphonic anhydride (T.sub.3P), a combination of 2,2-dipyridyl disulfide and triphenylphosphine, and the like known to those skilled in the art.

[0260] Coupling is typically achieved in an organic solvent such as, but not limited to, N,N-dimethylformamide (DMF), dichloromethane (DCM), acetonitrile, and tetrahydrofuran (THF) in the presence of base, such as, but not limited to diisopropylethylamine, triethylamine, and N-methylmorpholine.

[0261] Coupling reactions may be conducted with or without DMAP (in catalytic, stoichiometric, or superstoichiometric amounts, but more specifically catalytic amounts) at temperatures ranging from 78 C. to about 120 C., but specifically from from 20 C. to 50 C., and more specifically from 5 C. to 30 C.

[0262] Cross-coupling reactions useful in synthesizing the compounds of the invention include, but are not limited to, Suzuki coupling, Negishi coupling, Stille coupling, Kumada coupling, and Hiyama coupling.

[0263] A cross-coupling reaction generally requires a metal catalyst or a mixture of metal catalysts. Suitable metal catalysts include, but are not limited to, palladium catalysts, copper catalysts, nickel catalysts, iron catalysts, silver catalysts, gold catalysts, or a combination of two or more of these catalysts. Suitable palladium catalysts include, but are not limited to, palladium on carbon (Pd/C), palladium acetate (Pd(OAc).sub.2), tetrakis(triphenylphosphine)palladium(0) (Pd(PPh.sub.3).sub.4), bis(triphenylphosphine)palladium(II) dichloride (PdCl.sub.2(PPh.sub.3).sub.2), [1,1-bis(diphenylphosphino)ferrocene]palladium(II) dichloride ((dppf)PdCl.sub.2), and tris(dibenzylideneacetone)dipalladium(0) (Pd.sub.2(dba).sub.3). Suitable copper catalysts include, but are not limited to, CuCl, CuBr, CuI, Cu.sub.2O, CuOTf, Cu(MeCN).sub.4PF.sub.6, CuTC (copper(I) thiophene-2-carboxylate), Cu(OAc).sub.2, and Cu(OTf).sub.2. Suitable nickel catalysts include, but are not limited to, bis(cyclooctadiene)nickel(0), bis(triphenylphosphine)nickel chloride, [1,2-bis(diphenylphosphino)ethane]dichloronickel(II) ((dppe)NiCl.sub.2), [1,1-bis(diphenylphosphino)ferrocene]dichloronickel(II) ((dppf)NiCl.sub.2), and [1,3-bis(diphenylphosphino)propane]dichloronickel(II) ((1,3-dppp)NiCl.sub.2). Suitable iron catalysts include, but are not limited to, FeCl.sub.2, FeCl.sub.3, Fe(acac).sub.3, and Fe(OAc).sub.2. Suitable silver catalysts include, but are not limited to, Ag(OAc), AgOTf, AgPF, and AgClO.sub.4. Suitable gold catalysts include, but are not limited to, chloro(triphenylphosphine)gold(I) ((Ph.sub.3P)AuCl), chloro[1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene]gold(I), methyl(triphenylphosphine)gold(I), chloro[1,3-bis(2,4,6-trimethylphenyl)imidazol-2-ylidene]gold(I), and chloro(trimethylphosphine)gold(I).

[0264] The cross-coupling reaction may be carried out in a suitable solvent at temperatures between 78 C., and 250 C., more specifically at 0 C. to 120 C.

[0265] A suitable solvent for a cross-coupling reaction may be, but is not limited to, MeOH, EtOH, isopropanol, tert-butanol, H.sub.2O, DMF, DMSO, THF, 1,4-dioxane, 1,2-dimethoxyethane, or a mixture of two or more of these solvents.

[0266] The cross-coupling reaction may be performed under conventional heating or in a microwave reactor. Certain cross-coupling reactions are carried out under nitrogen or argon atmosphere. Other cross-coupling reactions may require the presence of air or oxygen. Additionally, base may be necessary for some cross-coupling reactions. Suitable bases include, but are not limited to, AgO, K.sub.2CO, tBuOK, tBuONa, Cs.sub.2CO.sub.3, and K.sub.3PO.sub.4.

[0267] Reactive groups for Suzuki cross-coupling reactions (referred to B.sup.1 and B.sup.2 in the below schemes) are typically (1) a boronic acid, bororic ester or a trifluoroborate salt moiety, such as but not limited to B(OH).sub.2, B(OMe).sub.2, B(OEt).sub.2, B(OPr-i).sub.2, B(pinacolato), and BF.sub.3K; (2) a halogen or a sulfonic ester group, such as but not limited to Cl, Br, I, O.sub.3SCF.sub.3, O.sub.3SC.sub.6H.sub.4Me-p, and O.sub.3SC.sub.8H.sub.5.

[0268] Various protecting groups (PG) are used in these schemes. Suitable amine protecting group including, but not limited to, tert-butyloxycarbonyl (Boc), carbobenzyloxy (Cbz), p-methoxybenzyl carbonyl (Moz), allyloxycarbonyl (Alloc), 9-fluorenylmethyloxycarbonyl (Fmoc), p-methoxybenzyl (PMB), 3,4-dimethoxybenzyl (DMPM), benzoyl (Bz), acetyl (Ac), methanesulfonyl (Ms), trifluoromethanesulfonyl (Tf), p-toluenesulfonyl (Ts), and 4-nitrobenzenesulfonyl (Nosyl). In certain embodiments, the amine protecting group is tert-butyloxycarbonyl (Boc). Suitable alcohol protecting groups include, but are not limited to, silyl groups (including, but not limited to, SiMe.sub.3, SiEt.sub.3, Si(iso-Pr).sub.3, SiMe.sub.2(tert-Bu), SiPh.sub.2(tert-Bu), -SEM (2-(trimethylsilyl)ethoxymethyl)), ether groups (including, but not limited to, -MOM (methoxymethyl), -MEM (2-methoxyethoxymethyl), -BOM (benzyloxymethyl), -PMBM (p-methoxybenzyloxymethyl), and -THP (tetrahydropyranyl)), and ester groups (including, but not limited to, acetate (Ac), formate, pivaloate (Pv), and benzoate). In certain embodiments, the alcohol protecting group is acetyl. Some protecting groups are an alkyl or any aryl group, such as but not limited to methyl, ethyl, n-propyl, isopropyl, tert-butyl, benzyl, p-methoxybenzyl, allyl, phenyl, and p-nitrophenyl. In some embodiments, the alkyl or aryl protecting group is methyl.

[0269] The removal of a protecting group may be carried out under basic or acidic conditions, depending upon the nature of the protecting group. Which conditions are applicable to specific protecting groups is well-known in the art. Suitable bases for protecting group removal include, but are not limited to, LiOH, NaOH, KOH, CsOH, Li.sub.2CO.sub.3, Na.sub.2CO.sub.3, K.sub.2CO.sub.3, Cs.sub.2CO.sub.3, and CsF. Suitable acids for protecting group removal include, but are not limited to, HCl, HBr, HI, H.sub.2SO.sub.4, HNO.sub.3, and CF.sub.3CO.sub.2H. Certain protecting groups may also be removed by using conditions or reagents, such as trimethyltin hydroxide, ceric ammonium nitrate, and oxalyl chloride.

[0270] Protecting group removal is typically out in a suitable solvent at temperatures between 78 C., and about 150 C., specifically at 0 C. to 120 C., more specifically at 0 C. to 25 C. A suitable solvent for such reaction includes, but is not limited to, MeOH, EtOH, isopropanol, tert-butanol, H.sub.2O, dichloromethane, ethyl acetate, DMF, DMSO, THF, 1,4-dioxane, and 1,2-dimethoxyethane.

[0271] Variables such as Q, X, ring Z, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8, p and r have the meaning set forth in Formula I.

[0272] Dipeptide A-1 may be prepared as shown in Scheme 1 below where the structural variables are as described above.

##STR00137##

[0273] In a typical procedure, intermediate A-A is reacted with intermediate A-B in the presence of a coupling agent.

Method A

[0274] Method A may be used to prepare compounds of Formula I as shown in Scheme 2 below. The structural variables of Formula I are as defined above.

##STR00138## ##STR00139##

[0275] Step 1:

[0276] Intermediate A-3 is synthesized from intermediates A-1 and A-2 via a cross-coupling reaction. In some embodiments, the cross-coupling reaction is a Suzuki coupling reaction. PG.sup.1 is a suitable amine protecting group. In certain embodiments, PG.sup.1 is tert-butyloxycarbonyl (Boc).

[0277] Step 2:

[0278] PG.sup.2 is an alkyl or aryl protecting group. In some embodiments, PG.sup.2 is methyl.

[0279] Intermediate A-4 may be synthesized from A-3 using a deprotecting reaction that hydrolyzes the PG.sup.2-containing ester into its corresponding acid.

[0280] Step 3:

[0281] The macrocyclization of intermediate A-4 gives intermediate A-5 is achieved by a cross-coupling reaction.

[0282] Alternatively, the cyclization reaction may be carried out by converting the acid group (CO.sub.2H) in the precursor to the corresponding acid chloride (COCl) using a chlorinating reagent (including, but not limited to, thionyl chloride, PCl.sub.3, PCl.sub.5, and oxalyl chloride with catalytic DMF) in a suitable solvent at temperatures between 78 C. and 120 C., preferably at 0 C. A suitable solvent includes, but is not limited to, DMF, dichloromethane, tetrahydrofuran, 1,2-dimethoxyethane, acetonitrile, ethyl acetate, toluene, and 1,4-dioxane. Following formation of the acid chloride, the solvent may be removed under reduced pressure and replaced with an alternative solvent including, but not limited to, N,N-dimethylformamide, dichloromethane, 1,2-dimethoxyethane, acetonitrile, tetrahydrofuran, and 1,2-dichloroethane. Addition of base (including, but not limited to, pyridine, diisopropylethylamine, triethylamine, N-methylmorpholine, and the like) is followed to form the cyclized product. The reaction temperatures range from 78 C. to 120 C., preferably between 20 C., and 50 C.

[0283] Step 4:

[0284] Intermediate A6 is synthesized from intermediate A-5 by removing PG.sup.1.

[0285] Step 5:

[0286] A compound of Formula I is synthesized from intermediates A-6 and A-7 via an amide formation reaction using a cross-coupling reaction. T

[0287] Alternatively, those skilled in the art will appreciate that a compound of Formula I may be synthesized by converting intermediate A-7 to its acyl chloride or acyl fluoride or activated ester or anhydride, and reacting with intermediate A-6. Examples of these types of reactions are available in the literature, such as Compendium of Organic Synthetic Methods, Vol. I-VI (Wiley-Interscience); or the Comprehensive Organic Transformations, by R.C. Larock (Wiley-Interscience).

Method B

[0288] Method B may alternatively be used to synthesize compounds of Formula I as shown in Scheme 3 below. The structural variables of Formula I are as defined above.

##STR00140## ##STR00141##

[0289] Step 1:

[0290] PG.sup.11 is a suitable alcohol (when YO) or amine (when YNH or N(C.sub.1-C.sub.3 alkyl)) protecting group.

[0291] Intermediate B-2 is synthesized from intermediates A-1 and B-1 via a cross-coupling reaction.

[0292] Step 2:

[0293] Intermediate A-3 may be synthesized from Intermediate B-2 by removing the PG.sup.11 group from the alcoholic oxygen atom to which it is attached or from an amino nitrogen atom to which it is attached.

[0294] Steps 3-6:

[0295] The conversion of Intermediate A-3 to Formula I is detailed in the description of Method A.

Method C

[0296] Method C may alternatively be used to synthesize compounds of Formula I as shown in Scheme 4 below. The structural variables of Formula I are as defined above.

##STR00142## ##STR00143##

[0297] Step 1:

[0298] Intermediate C-1 may be synthesized from A-1 using a deprotecting reaction that hydrolyzes the PG.sup.2-containing ester to its corresponding acid.

[0299] Step 2:

[0300] Intermediate C-2 may be synthesized from C-1 via amide formation using a cross-coupling reaction.

[0301] Alternatively, those skilled in the art will appreciate that a compound of Formula I may be synthesized by converting intermediate A-7 to its acyl chloride, acyl fluoride, activated ester or anhydride, and reacting with intermediate A-2. Examples of these types of reactions are available in literature, such as Compendium of Organic Synthetic Methods, Vol. I-VI (Wiley-Interscience); or the Comprehensive Organic Transformations, by R.C. Larock (Wiley-Interscience).

[0302] Step 3: Macrocycle A-5 may be synthesized from Intermediate C-2 using a cross-coupling reaction. In some embodiments, the cross-coupling reaction is a Suzuki coupling reaction.

[0303] Steps 4-5: The conversion of macrocycle A-5 to Formula I is detailed in the description of Method A.

Proteins

Presenter Proteins

[0304] Presenter proteins can bind a compound of the invention to form a complex, which can bind to and modulate the activity of a mutant RAS target protein. The presenter protein is a member of the cyclophilin A family (e.g., CYPA, CYPB, CYPC, CYP40, CYPE, CYPD, NKTR, SRCyp, CYPH, CWC27, CYPL1, CYP60, CYPJ, PPIL4, PPIL6, RANBP2, PPWD1, PPIAL4A, PPIAL4B, PPIAL4C, PPIAL4D, or PPIAL4G).

[0305] The cyclophilin family is a family of proteins that bind to cyclosporine. Genes that encode proteins in this family include PPIA, PPIB, PPIC, PPID, PPIE, PPIF, PPIG, PPIH, SDCCAG-10, PPIL1, PPIL2, PPIL3, PPIL4, P270, PPWD1, and COAS-2. Exemplary cyclophilins include CYPA, CYPB, CYPC, CYP40, CYPE, CYPD, NKTR, SRCyp, CYPH, CWC27, CYPL1, CYP60, CYPJ, PPIL4, PPIL6, RANBP2, PPWD1, PPIAL4A, PPIAL4B, PPIAL4C, PPIAL4D, and PPIAL4G.

[0306] Representative presenter proteins are encoded by the genes or homologs thereof listed in Table 1; in some embodiments, a reference presenter protein is encoded by a gene set forth in Table 1. Also, those of ordinary skill in the art, referring to Table 1, can readily identify sequences that are characteristic of presenter proteins generally, and/or of particular subsets of presenter proteins.

TABLE-US-00001 TABLE 1 Genes that Encode Selected Presenter Proteins Gene Name Uniprot Accession Number PPIA Q567Q0 PPIB P23284 PPIC P45877 PPID Q08752 PPIE Q9UNP9 PPIG Q13427 PPIH O43447 PPIL1 Q9Y3C6 PPIL2 Q13356 PPIL3 Q9H2H8 PPIL4 Q8WUA2 PPIL5 Q32Q17 PPIL6 Q8IXY8 PPWD1 Q96BP3

Target Proteins

[0307] A target protein (e.g., a eukaryotic target protein such as a mammalian target protein) is a protein which mediates a disease condition or a symptom of a disease condition. As such, a desirable therapeutic effect can be achieved by modulating (inhibiting or increasing) its activity. Target proteins useful in the complexes and methods of the invention include those which do not naturally associate with a presenter protein, e.g., those which have an affinity for a presenter protein in the absence of a binary complex with a compound of the invention of greater than 1 M, preferably greater than 5 M, and more preferably greater than 10 M. Alternatively, target proteins which do not naturally associate with a presenter protein are those which have an affinity for a compound of the invention in the absence of a binary complex greater than 1 M, preferably greater than 5 M, and more preferably greater than 10 M. In yet another alternative, target proteins which do not naturally associate with a presenter protein are those which are other than calcineurin or mTOR.

[0308] Target proteins can be naturally occurring, e.g., wild type. Alternatively, a target protein can vary from the wild type protein but still retain biological function, e.g., as a mutant, a splice variant or a biologically active fragment.

[0309] In some embodiments, the target protein is a RAS family protein.

[0310] In some embodiments, the target protein is a KRAS protein. In some embodiments, the KRAS protein is a KRAS G12C protein. In some embodiments, the KRAS protein is a KRAS G13C protein.

[0311] In some embodiments, the target protein is an NRAS protein. In some embodiments, the NRAS protein is an NRAS G12C protein. In some embodiments, the NRAS protein is an NRAS G13C protein.

[0312] In some embodiments, the target protein is an HRAS protein. In some embodiments, the HRAS protein is an HRAS G12C protein. In some embodiments, the HRAS protein is an HRAS G13C protein.

Complexes

Presenter Protein/Compound Complexes

[0313] In one aspect, the invention provides a complex comprising a compound of the invention, a CYPA family member presented protein and a mutant RAS protein.

[0314] In a related aspect, this disclosure features a method of producing the above-described complex, the method including contacting a CYPA family member presenter protein and a mutant RAS protein with a compound of the present invention, any pharmaceutical composition comprising such a compound, under conditions suitable to permit complex formation.

[0315] In some embodiments of either of the above two aspects, the mutated RAS protein is KRAS G12C, NRAS G12C, or HRAS G12C. In some embodiments, the mutated RAS protein is KRAS G13C, NRAS G13C, or HRAS G13C. In some embodiments, the mutated RAS protein is KRAS G12C.

[0316] In some embodiments of either of the above two aspects, the presenter protein is CYPA, CYPB, CYPC, CYP40, CYPE, CYPD, NKTR, SRCyp. CYPH, CWC27, CYPL1, CYP60, CYPJ, PPIL4, PPIL6, RANBP2, or PPWD1. In some embodiments, the presenter protein is CYPA.

[0317] In some embodiments, a presenter protein/compound/target protein complex of the invention inhibits a naturally occurring interaction between a target protein and a ligand, such as a protein or a small molecule that specifically binds to the target protein.

[0318] In some embodiments, a presenter protein/compound/target protein complex of the invention inhibits the binding of BRAF to the mutant RAS (e.g., KRAS G12C, KRAS G13C, NRAS G12C, NRAS G13C, HRAS G12C, or HRAS G13C).

Kits

[0319] In some embodiments, the present invention relates to a kit for conveniently and effectively carrying out the methods in accordance with the present invention. In general, the pharmaceutical pack or kit comprises one or more containers filled with one or more of the ingredients of the pharmaceutical compositions of the invention. Such kits are especially suited for the delivery of solid oral forms such as tablets or capsules. Such a kit preferably includes a number of unit dosages, and may also include a card having the dosages oriented in the order of their intended use. If desired, for instance if the subject suffers from Alzheimer's disease, a memory aid can be provided, for example in the form of numbers, letters, or other markings or with a calendar insert, designating the days in the treatment schedule in which the dosages can be administered. Alternatively, placebo dosages, or calcium dietary supplements, either in a form similar to or distinct from the dosages of the pharmaceutical compositions, can be included to provide a kit in which a dosage is taken every day. Optionally associated with such container(s) can be a notice in the form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceutical products, which notice reflects approval by the agency of manufacture, use or sale for human administration.

Pharmaceutical Compositions

[0320] For use as treatment of human and animal subjects, the compounds of the invention can be formulated as pharmaceutical or veterinary compositions. Depending on the subject to be treated, the mode of administration, and the type of treatment desirede.g., prevention, prophylaxis, or therapythe compounds are formulated in ways consonant with these parameters. A summary of such techniques is found in Remington: The Science and Practice of Pharmacy. 21.sup.51 Edition, Lippincott Williams & Wilkins, (2005); and Encyclopedia of Pharmaceutical Technology, eds. J. Swarbrick and J. C. Boylan, 1988-1999, Marcel Dekker, New York, each of which is incorporated herein by reference.

[0321] Compounds described herein may be present in amounts totaling 1-95% by weight of the total weight of the composition. The composition may be provided in a dosage form that is suitable for intraarticular, oral, parenteral (e.g., intravenous, intramuscular), rectal, cutaneous, subcutaneous, topical, transdermal, sublingual, nasal, vaginal, intravesicular, intraurethral, intrathecal, epidural, aural, or ocular administration, or by injection, inhalation, or direct contact with the nasal, genitourinary, reproductive or oral mucosa. Thus, the pharmaceutical composition may be in the form of, e.g., tablets, capsules, pills, powders, granulates, suspensions, emulsions, solutions, gels including hydrogels, pastes, ointments, creams, plasters, drenches, osmotic delivery devices, suppositories, enemas, injectables, implants, sprays, preparations suitable for iontophoretic delivery, or aerosols. The compositions may be formulated according to conventional pharmaceutical practice.

[0322] In general, for use in treatment, compounds described herein may be used alone, or in combination with one or more other active agents. An example of other pharmaceuticals to combine with the compounds described herein would include pharmaceuticals for the treatment of the same indication. Another example of a potential pharmaceutical to combine with compounds described herein would include pharmaceuticals for the treatment of different yet associated or related symptoms or indications. Depending on the mode of administration, compounds will be formulated into suitable compositions to permit facile delivery. Each compound of a combination therapy may be formulated in a variety of ways that are known in the art. For example, the first and second agents of the combination therapy may be formulated together or separately. Desirably, the first and second agents are formulated together for the simultaneous or near simultaneous administration of the agents.

[0323] Compounds of the invention may be prepared and used as pharmaceutical compositions comprising an effective amount of a compound described herein and a pharmaceutically acceptable carrier or excipient, as is well known in the art. In some embodiments, a composition includes at least two different pharmaceutically acceptable excipients or carriers.

[0324] Formulations may be prepared in a manner suitable for systemic administration or topical or local administration. Systemic formulations include those designed for injection (e.g., intramuscular, intravenous or subcutaneous injection) or may be prepared for transdermal, transmucosal, or oral administration. A formulation will generally include a diluent as well as, in some cases, adjuvants, buffers, preservatives and the like. Compounds can be administered also in liposomal compositions or as microemulsions.

[0325] For injection, formulations can be prepared in conventional forms as liquid solutions or suspensions or as solid forms suitable for solution or suspension in liquid prior to injection or as emulsions. Suitable excipients include, for example, water, saline, dextrose, glycerol and the like. Such compositions may also contain amounts of nontoxic auxiliary substances such as wetting or emulsifying agents, pH buffering agents and the like, such as, for example, sodium acetate, sorbitan monolaurate, and so forth.

[0326] Various sustained release systems for drugs have also been devised. See, for example, U.S. Pat. No. 5,624,677, which is herein incorporated by reference.

[0327] Systemic administration may also include relatively noninvasive methods such as the use of suppositories, transdermal patches, transmucosal delivery and intranasal administration. Oral administration is also suitable for compounds of the invention. Suitable forms include syrups, capsules, and tablets, as is understood in the art.

[0328] Each compound of a combination therapy, as described herein, may be formulated in a variety of ways that are known in the art. For example, the first and second agents of the combination therapy may be formulated together or separately.

[0329] The individually or separately formulated agents can be packaged together as a kit. Non-limiting examples include, but are not limited to, kits that contain, e.g., two pills, a pill and a powder, a suppository and a liquid in a vial, two topical creams, etc. The kit can include optional components that aid in the administration of the unit dose to subjects, such as vials for reconstituting powder forms, syringes for injection, customized IV delivery systems, inhalers, etc. Additionally, the unit dose kit can contain instructions for preparation and administration of the compositions. The kit may be manufactured as a single use unit dose for one subject, multiple uses for a particular subject (at a constant dose or in which the individual compounds may vary in potency as therapy progresses); or the kit may contain multiple doses suitable for administration to multiple subjects (bulk packaging). The kit components may be assembled in cartons, blister packs, bottles, tubes, and the like.

[0330] Formulations for oral use include tablets containing the active ingredient(s) in a mixture with non-toxic pharmaceutically acceptable excipients. These excipients may be, for example, inert diluents or fillers (e.g., sucrose, sorbitol, sugar, mannitol, microcrystalline cellulose, starches including potato starch, calcium carbonate, sodium chloride, lactose, calcium phosphate, calcium sulfate, or sodium phosphate); granulating and disintegrating agents (e.g., cellulose derivatives including microcrystalline cellulose, starches including potato starch, croscarmellose sodium, alginates, or alginic acid); binding agents (e.g., sucrose, glucose, sorbitol, acacia, alginic acid, sodium alginate, gelatin, starch, pregelatinized starch, microcrystalline cellulose, magnesium aluminum silicate, carboxymethylcellulose sodium, methylcellulose, optionally substituted hydroxylpropyl methylcellulose, ethylcellulose, polyvinylpyrrolidone, or polyethylene glycol); and lubricating agents, glidants, and antiadhesives (e.g., magnesium stearate, zinc stearate, stearic acid, silicas, hydrogenated vegetable oils, or talc). Other pharmaceutically acceptable excipients can be colorants, flavoring agents, plasticizers, humectants, buffering agents, and the like.

[0331] Two or more compounds may be mixed together in a tablet, capsule, or other vehicle, or may be partitioned. In one example, the first compound is contained on the inside of the tablet, and the second compound is on the outside, such that a substantial portion of the second compound is released prior to the release of the first compound.

[0332] Formulations for oral use may also be provided as chewable tablets, or as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent (e.g., potato starch, lactose, microcrystalline cellulose, calcium carbonate, calcium phosphate or kaolin), or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example, peanut oil, liquid paraffin, or olive oil. Powders, granulates, and pellets may be prepared using the ingredients mentioned above under tablets and capsules in a conventional manner using, e.g., a mixer, a fluid bed apparatus or a spray drying equipment.

[0333] Dissolution or diffusion-controlled release can be achieved by appropriate coating of a tablet, capsule, pellet, or granulate formulation of compounds, or by incorporating the compound into an appropriate matrix. A controlled release coating may include one or more of the coating substances mentioned above and/or, e.g., shellac, beeswax, glycowax, castor wax, camauba wax, stearyl alcohol, glyceryl monostearate, glyceryl distearate, glycerol palmitostearate, ethylcellulose, acrylic resins, dl-polylactic acid, cellulose acetate butyrate, polyvinyl chloride, polyvinyl acetate, vinyl pyrrolidone, polyethylene, polymethacrylate, methylmethacrylate, 2-optionally substituted hydroxylmethacrylate, methacrylate hydrogels, 1,3 butylene glycol, ethylene glycol methacrylate, and/or polyethylene glycols. In a controlled release matrix formulation, the matrix material may also include, e.g., hydrated methylcellulose, camauba wax and stearyl alcohol, carbopol 934, silicone, glyceryl tristearate, methyl acrylate-methyl methacrylate, polyvinyl chloride, polyethylene, and/or halogenated fluorocarbon.

[0334] The liquid forms in which the compounds and compositions of the present invention can be incorporated for administration orally include aqueous solutions, suitably flavored syrups, aqueous or oil suspensions, and flavored emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil, or peanut oil, as well as elixirs and similar pharmaceutical vehicles.

[0335] Generally, when administered to a human, the oral dosage of any of the compounds of the combination of the invention will depend on the nature of the compound, and can readily be determined by one skilled in the art. Typically, such dosage is normally about 0.001 mg to 2000 mg per day, desirably about 1 mg to 1000 mg per day, and more desirably about 5 mg to 500 mg per day. Dosages up to 200 mg per day may be necessary.

[0336] Administration of each drug in a combination therapy, as described herein, can, independently, be one to four times daily for one day to one year, and may even be for the life of the subject. Chronic, long-term administration may be indicated.

[0337] The following examples are intended to illustrate the synthesis of a representative number of compounds and the use of these compounds for the ternary complex formation between CYPA and KRAS G12C. Accordingly, the examples are intended to illustrate but not to limit the invention. Additional compounds not specifically exemplified may be synthesized using conventional methods in combination with the methods described herein. Moreover, other RAS proteins, such as KRAS G13C, NRAS G12C, NRAS G13C, HRAS G12C or HRAS G13C, may be employed for ternary complex formation.

Methods of Treatment

[0338] In an aspect, the invention discloses a method of treating a disease or disorder that is characterized by aberrant RAS activity due to a RAS mutant. In some embodiments, the disease or disorder is a cancer. In some embodiments, the cancer is pancreatic cancer, colorectal cancer, non-small cell lung cancer, small cell lung cancer, acute myeloid leukemia, multiple myeloma, thyroid gland adenocarcinoma, a myelodysplastic syndrome, or squamous cell lung carcinoma. In some embodiments, the aberrant RAS activity is due to a RAS G12C mutation. In some embodiments, the aberrant RAS activity is due to a RAS G13C mutation. In some embodiments, the aberrant RAS activity is due to a KRAS G12C mutation. In some embodiments, the aberrant RAS activity is due to a KRAS G13C mutation. In some embodiments, the aberrant RAS activity is due to an HRAS G12C mutation. In some embodiments, the aberrant RAS activity is due to an HRAS G13C mutation. In some embodiments, the aberrant RAS activity is due to an HRAS G12C mutation. In some embodiments, the aberrant RAS activity is due to an HRAS G13C mutation.

[0339] In an aspect, the invention discloses a method of treating a disease or disorder that is characterized by aberrant or unwanted BRAF-RAS binding, the method including contacting the cell with a compound of the present invention, any pharmaceutical composition comprising such a compound. In some embodiments, the disease is characterized by aberrant or unwanted binding between BRAF and a mutant RAS protein. In some embodiments, the disease or disorder is a cancer. In some embodiments, the cancer is pancreatic cancer, colorectal cancer, non-small cell lung cancer, small cell lung cancer, acute myeloid leukemia, multiple myeloma, thyroid gland adenocarcinoma, a myelodysplastic syndrome, or squamous cell lung carcinoma. In some embodiments, the aberrant RAS activity is due to a RAS G12C mutation. In some embodiments, the aberrant RAS activity is due to a RAS G13C mutation. In some embodiments, the aberrant RAS activity is due to a KRAS G12C mutation. In some embodiments, the aberrant RAS activity is due to a KRAS G13C mutation. In some embodiments, the aberrant RAS activity is due to an NRAS G12C mutation. In some embodiments, the aberrant RAS activity is due to an NRAS G13C mutation. In some embodiments, the aberrant RAS activity is due to an HRAS G12C mutation. In some embodiments, the aberrant RAS activity is due to an HRAS G13C mutation.

[0340] In an aspect, the invention discloses a method of treating a disease or disorder that is characterized by aberrant or unwanted pERK expression, the method including contacting the cell with an effective amount of a compound of the present invention, any pharmaceutical composition comprising such a compound. In some embodiments, the aberrant or unwanted pERK expression is driven by a mutant RAS protein. In some embodiments, the disease or disorder is a cancer. In some embodiments, the cancer is pancreatic cancer, colorectal cancer, non-small cell lung cancer, small cell lung cancer, acute myeloid leukemia, multiple myeloma, thyroid gland adenocarcinoma, a myelodysplastic syndrome, or squamous cell lung carcinoma. In some embodiments, the mutant RAS that drives pERK expression has a G12C mutation. In some embodiments, the mutant RAS that drives pERK expression has a G13C mutation. In some embodiments, the mutant RAS activity is due to a KRAS G12C mutation. In some embodiments, the mutant RAS activity is due to a KRAS G13C mutation. In some embodiments, the mutant RAS activity is due to an NRAS G12C mutation. In some embodiments, the mutant RAS that drives pERK expression is due to an NRAS G13C mutation. In some embodiments, the mutant RAS activity is due to a KRAS G12C mutation. In some embodiments, the mutant RAS that drives pERK expression is due to a KRAS G13C mutation.

[0341] In some embodiments, the compounds of the present invention or pharmaceutically acceptable salts thereof, pharmaceutical compositions comprising such compounds or salts, and methods provided herein may be used for the treatment of a wide variety of cancers including tumors such as lung, prostate, breast, brain, skin, cervical carcinomas, testicular carcinomas, etc. More particularly, cancers that may be treated by the compounds or salts thereof, pharmaceutical compositions comprising such compounds or salts, and methods of the invention include, but are not limited to tumor types such as astrocytic, breast, cervical, colorectal, endometrial, esophageal, gastric, head and neck, hepatocellular, laryngeal, lung, oral, ovarian, prostate and thyroid carcinomas and sarcomas. Other cancers include, for example: [0342] Cardiac, for example: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; [0343] Lung, for example: bronchogenic carcinoma (squamous cell, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; [0344] Gastrointestinal, for example: esophagus (squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcoma), pancreas (ductal adenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors, vipoma), small bowel (adenocarcinoma, lymphoma, carcinoid tumors, Kaposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, fibroma), large bowel (adenocarcinoma, tubular adenoma, villous adenoma, hamartoma, leiomyoma); [0345] Genitourinary tract, for example: kidney (adenocarcinoma, Wilm's tumor (nephroblastoma), lymphoma, leukemia), bladder and urethra (squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma), prostate (adenocarcinoma, sarcoma), testis (seminoma, teratoma, embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors, lipoma); [0346] Liver, for example: hepatoma (hepatocellular carcinoma), cholangiocarcinoma, hepatoblastoma, angiosarcoma, hepatocellular adenoma, hemangioma; [0347] Biliary tract, for example: gall bladder carcinoma, ampullary carcinoma, cholangiocarcinoma; [0348] Bone, for example: osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cell sarcoma), multiple myeloma, malignant giant cell tumor chordoma, osteochronfroma (osteocartilaginous exostoses), benign chondroma, chondroblastoma, chondromyxofibroma, osteoid osteoma and giant cell tumors; [0349] Nervous system, for example: skull (osteoma, hemangioma, granuloma, xanthoma, osteitis deformans), meninges (meningioma, meningiosarcoma, gliomatosis), brain (astrocytoma, medulloblastoma, glioma, ependymoma, germinoma (pinealoma), glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, congenital tumors), spinal cord neurofibroma, neurofibromatosis type 1, meningioma, glioma, sarcoma); [0350] Gynecological, for example: uterus (endometrial carcinoma, uterine carcinoma, uterine corpus endometrial carcinoma), cervix (cervical carcinoma, pre-tumor cervical dysplasia), ovaries (ovarian carcinoma (serous cystadenocarcinoma, mucinous cystadenocarcinoma, unclassified carcinoma), granulosa-thecal cell tumors. Sertoli-Leydig cell tumors, dysgerminoma, malignant teratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma), fallopian tubes (carcinoma); [0351] Hematologic, for example: blood (myeloid leukemia (acute and chronic), acute lymphoblastic leukemia, chronic lymphocytic leukemia, myeloproliferative diseases, multiple myeloma, myelodysplastic syndrome), Hodgkin's disease, non-Hodgkin's lymphoma (malignant lymphoma); [0352] Skin, for example: malignant melanoma, basal cell carcinoma, squamous cell carcinoma, Kaposi's sarcoma, moles dysplastic nevi, lipoma, angioma, dermatofibroma, keloids, psoriasis; and [0353] Adrenal glands, for example: neuroblastoma.

[0354] Also provided is a method of inhibiting a Ras protein in a cell, the method comprising contacting the cell with an effective amount of a compound of the present invention, or a pharmaceutically acceptable salt thereof. A method of inhibiting RAF-Ras binding, the method comprising contacting the cell with an effective amount of a compound of the present invention, or a pharmaceutically acceptable salt thereof, is also provided. The cell may be a cancer cell. The cancer cell may be of any type of cancer described herein.

[0355] Combination Therapies

[0356] It will be appreciated that the compounds and pharmaceutical compositions of the present invention can be formulated and employed in combination therapies, that is, the compounds and pharmaceutical compositions can be formulated with or administered concurrently with, prior to, or subsequent to, one or more other desired therapeutics or medical procedures. The particular combination of therapies (therapeutics or procedures) to employ in a combination regimen will take into account compatibility of the desired therapeutics and/or procedures and the desired therapeutic effect to be achieved. It will also be appreciated that the therapies employed may achieve a desired effect for the same disorder, or they may achieve different effects (e.g., control of any adverse effects).

[0357] In some embodiments of the methods described herein, the methods may further include an additional therapeutic agent. For example, the methods of the invention may include a compound of the invention used alone or in combination with one or more additional therapies (e.g., non-drug treatments or therapeutic agents). The dosages of one or more of the additional therapies (e.g., non-drug treatments or therapeutic agents) may be reduced from standard dosages when administered alone. For example, doses may be determined empirically from drug combinations and permutations or may be deduced by isobolographic analysis (e.g., Black et al., Neurology 65:S3-S6 (2005)).

[0358] A compound of the present invention may be administered before, after, or concurrently with one or more of such additional therapies. When combined, dosages of a compound of the invention and dosages of the one or more additional therapies (e.g., non-drug treatment or therapeutic agent) provide a therapeutic effect (e.g., synergistic or additive therapeutic effect). A compound of the present invention and an additional therapy, such as an anti-cancer agent, may be administered together, such as in a unitary pharmaceutical composition, or separately and, when administered separately, this may occur simultaneously or sequentially. Such sequential administration may be close or remote in time.

[0359] In some embodiments, the additional therapy is the administration of side-effect limiting agents (e.g., agents intended to lessen the occurrence or severity of side effects of treatment. For example, in some embodiments, the compounds of the present invention can also be used in combination with a therapeutic agent that treats nausea. Examples of agents that can be used to treat nausea include: dronabinol, granisetron, metoclopramide, ondansetron, and prochlorperazine, or pharmaceutically acceptable salts thereof.

[0360] In some embodiments, the one or more additional therapies includes a non-drug treatment (e.g., surgery or radiation therapy). In some embodiments, the one or more additional therapies includes a therapeutic agent (e.g., a compound or biologic that is an anti-angiogenic agent, signal transduction inhibitor, antiproliferative agent, glycolysis inhibitor, or autophagy inhibitor). In some embodiments, the one or more additional therapies includes a non-drug treatment (e.g., surgery or radiation therapy) and a therapeutic agent (e.g., a compound or biologic that is an anti-angiogenic agent, signal transduction inhibitor, antiproliferative agent, glycolysis inhibitor, or autophagy inhibitor). In other embodiments, the one or more additional therapies includes two therapeutic agents. In still other embodiments, the one or more additional therapies includes three therapeutic agents. In some embodiments, the one or more additional therapies includes four or more therapeutic agents.

[0361] Non-Drug Therapies

[0362] Examples of non-drug treatments include, but are not limited to, radiation therapy, cryotherapy, hyperthermia, surgery (e.g., surgical excision of tumor tissue), and T cell adoptive transfer (ACT) therapy.

[0363] In some embodiments, the compounds of the invention may be used as an adjuvant therapy after surgery. In some embodiments, the compounds of the invention may be used as a neo-adjuvant therapy prior to surgery.

[0364] Radiation therapy may be used for inhibiting abnormal cell growth or treating a hyperproliferative disorder, such as cancer, in a subject (e.g., mammal (e.g., human)). Techniques for administering radiation therapy are known in the art. Radiation therapy can be administered through one of several methods, or a combination of methods, including, without limitation, external-beam therapy, internal radiation therapy, implant radiation, stereotactic radiosurgery, systemic radiation therapy, radiotherapy and permanent or temporary interstitial brachy therapy. The term brachy therapy, as used herein, refers to radiation therapy delivered by a spatially confined radioactive material inserted into the body at or near a tumor or other proliferative tissue disease site. The term is intended, without limitation, to include exposure to radioactive isotopes (e.g., At-211, 1-131, I-125, Y-90, Re-186, Re-188, Sm-153, Bi-212, P-32, and radioactive isotopes of Lu). Suitable radiation sources for use as a cell conditioner of the present invention include both solids and liquids. By way of non-limiting example, the radiation source can be a radionuclide, such as I-125, I-131, Yb-169, Ir-192 as a solid source, I-125 as a solid source, or other radionuclides that emit photons, beta particles, gamma radiation, or other therapeutic rays. The radioactive material can also be a fluid made from any solution of radionuclide(s), e.g., a solution of I-125 or I-131, or a radioactive fluid can be produced using a slurry of a suitable fluid containing small particles of solid radionuclides, such as Au-198, or Y-90. Moreover, the radionuclide(s) can be embodied in a gel or radioactive micro spheres.

[0365] In some embodiments, the compounds of the present invention can render abnormal cells more sensitive to treatment with radiation for purposes of killing or inhibiting the growth of such cells. Accordingly, this invention further relates to a method for sensitizing abnormal cells in a mammal to treatment with radiation which comprises administering to the mammal an amount of a compound of the present invention, which amount is effective to sensitize abnormal cells to treatment with radiation. The amount of the compound in this method can be determined according to the means for ascertaining effective amounts of such compounds described herein. In some embodiments, the compounds of the present invention may be used as an adjuvant therapy after radiation therapy or as a neo-adjuvant therapy prior to radiation therapy.

[0366] In some embodiments, the non-drug treatment is a T cell adoptive transfer (ACT) therapy. In some embodiments, the T cell is an activated T cell. The T cell may be modified to express a chimeric antigen receptor (CAR). CAR modified T (CAR-T) cells can be generated by any method known in the art. For example, the CAR-T cells can be generated by introducing a suitable expression vector encoding the CAR to a T cell. Prior to expansion and genetic modification of the T cells, a source of T cells is obtained from a subject. T cells can be obtained from a number of sources, including peripheral blood mononuclear cells, bone marrow, lymph node tissue, cord blood, thymus tissue, tissue from a site of infection, ascites, pleural effusion, spleen tissue, and tumors. In certain embodiments of the present invention, any number of T cell lines available in the art may be used. In some embodiments, the T cell is an autologous T cell. Whether prior to or after genetic modification of the T cells to express a desirable protein (e.g., a CAR), the T cells can be activated and expanded generally using methods as described, for example, in U.S. Pat. Nos. 6,352,694; 6,534,055; 6,905,680; 6,692,964; 5,858,358; 6,887,466; 6,905,681; 7,144,575; 7,067,318; 7,172,869; 7,232,566; 7,175,843; 7,572,631; 5,883,223; 6,905,874; 6,797,514; and 6,867,041.

[0367] Therapeutic Agents

[0368] A therapeutic agent may be a compound used in the treatment of cancer or symptoms associated therewith.

[0369] For example, a therapeutic agent may be a steroid. Accordingly, in some embodiments, the one or more additional therapies includes a steroid. Suitable steroids may include, but are not limited to, 21-acetoxypregnenolone, alclometasone, algestone, amcinonide, beclomethasone, betamethasone, budesonide, chloroprednisone, clobetasol, clocortolone, cloprednol, corticosterone, cortisone, cortivazol, deflazacort, desonide, desoximetasone, dexamethasone, diflorasone, diflucortolone, difuprednate, enoxolone, fluazacort, fiucloronide, flumethasone, flunisolide, fluocinolone acetonide, fluocinonide, fluocortin butyl, fluocortolone, fluorometholone, fluperolone acetate, fluprednidene acetate, fluprednisolone, flurandrenolide, fluticasone propionate, formocortal, halcinonide, halobetasol propionate, halometasone, hydrocortisone, loteprednol etabonate, mazipredone, medrysone, meprednisone, methylprednisolone, mometasone furoate, paramethasone, prednicarbate, prednisolone, prednisolone 25-diethylaminoacetate, prednisolone sodium phosphate, prednisone, prednival, prednylidene, rimexolone, tixocortol, triamcinolone, triamcinolone acetonide, triamcinolone benetonide, triamcinolone hexacetonide, and salts or derivatives thereof.

[0370] Further examples of therapeutic agents that may be used in combination therapy with a compound of the present invention include compounds described in the following patents: U.S. Pat. Nos. 6,258,812, 6,630,500, 6,515,004, 6,713,485, 5,521,184, 5,770,599, 5,747,498, 5,990,141, 6,235,764, and 8,623,885, and International Patent Applications WO01/37820, WO01/32651, WO02/68406, WO02/66470, WO02/55501, WO04/05279, WO04/07481, WO04/07458, WO04/09784, WO02/59110, WO99/45009, WO00/59509, WO99/61422, WO00/12089, and WO00/02871.

[0371] A therapeutic agent may be a biologic (e.g., cytokine (e.g., interferon or an interleukin such as IL-2)) used in treatment of cancer or symptoms associated therewith. In some embodiments, the biologic is an immunoglobulin-based biologic, e.g., a monoclonal antibody (e.g., a humanized antibody, a fully human antibody, an Fc fusion protein, or a functional fragment thereof) that agonizes a target to stimulate an anti-cancer response or antagonizes an antigen important for cancer. Also included are antibody-drug conjugates.

[0372] A therapeutic agent may be a T-cell checkpoint inhibitor. In one embodiment, the checkpoint inhibitor is an inhibitory antibody (e.g., a monospecific antibody such as a monoclonal antibody). The antibody may be, e.g., humanized or fully human. In some embodiments, the checkpoint inhibitor is a fusion protein, e.g., an Fc-receptor fusion protein. In some embodiments, the checkpoint inhibitor is an agent, such as an antibody, that interacts with a checkpoint protein. In some embodiments, the checkpoint inhibitor is an agent, such as an antibody, that interacts with the ligand of a checkpoint protein. In some embodiments, the checkpoint inhibitor is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of CTLA-4 (e.g., an anti-CTLA-4 antibody or fusion a protein). In some embodiments, the checkpoint inhibitor is an inhibitor or antagonist (e.g., an inhibitory antibody or small molecule inhibitor) of PD-1. In some embodiments, the checkpoint inhibitor is an inhibitor or antagonist (e.g., an inhibitory antibody or small molecule inhibitor) of PDL-1. In some embodiments, the checkpoint inhibitor is an inhibitor or antagonist (e.g., an inhibitory antibody or Fc fusion or small molecule inhibitor) of PDL-2 (e.g., a PDL-2/Ig fusion protein). In some embodiments, the checkpoint inhibitor is an inhibitor or antagonist (e.g., an inhibitory antibody or small molecule inhibitor) of B7-H3, B7-H.sub.4, BTLA, HVEM, TIM3. GAL9, LAG3, VISTA, KIR, 2B4, CD160, CGEN-15049, CHK 1, CHK2, A2aR, B-7 family ligands, or a combination thereof. In some embodiments, the checkpoint inhibitor is pembrolizumab, nivolumab, PDR001 (NVS), REGN2810 (Sanofi/Regeneron), a PD-L1 antibody such as, e.g., avelumab, durvalumab, atezolizumab, pidilizumab, JNJ-63723283 (JNJ), BGB-A317 (BeiGene & Celgene) or a checkpoint inhibitor disclosed in Preusser, M, et al. (2015) Nat. Rev. Neurol., including, without limitation, ipilimumab, tremelimumab, nivolumab, pembrolizumab, AMP224, AMP514/MEDI0680, BMS936559, MED14736, MPDL3280A, MSB0010718C, BMS986016, IMP321, Iirilumab, IPH2101, 1-7F9, and KW-6002.

[0373] A therapeutic agent may be an anti-TIGIT antibody, such as MBSA43, BMS-986207, MK-7684, COM902, AB154, MTIG7192A or OMP-313M32 (etigilimab).

[0374] A therapeutic agent may be an agent that treats cancer or symptoms associated therewith (e.g., a cytotoxic agent, non-peptide small molecules, or other compound useful in the treatment of cancer or symptoms associated therewith, collectively, an anti-cancer agent). Anti-cancer agents can be, e.g., chemotherapeutics or targeted therapy agents.

[0375] Anti-cancer agents include mitotic inhibitors, intercalating antibiotics, growth factor inhibitors, cell cycle inhibitors, enzymes, topoisomerase inhibitors, biological response modifiers, alkylating agents, antimetabolites, folic acid analogs, pyrimidine analogs, purine analogs and related inhibitors, vinca alkaloids, epipodopyyllotoxins, antibiotics, L-Asparaginase, topoisomerase inhibitors, interferons, platinum coordination complexes, anthracenedione substituted urea, methyl hydrazine derivatives, adrenocortical suppressant, adrenocorticosteroides, progestins, estrogens, antiestrogen, androgens, antiandrogen, and gonadotropin-releasing hormone analog. Further anti-cancer agents include leucovorin (LV), irenotecan, oxaliplatin, capecitabine, paclitaxel, and doxetaxel. In some embodiments, the one or more additional therapies includes two or more anti-cancer agents. The two or more anti-cancer agents can be used in a cocktail to be administered in combination or administered separately. Suitable dosing regimens of combination anti-cancer agents are known in the art and described in, for example, Saltz et al., Proc. Am. Soc. Clin. Oncol, 18:233a (1999), and Douillard et al., Lancet 355(9209):1041-1047 (2000).

[0376] Other non-limiting examples of anti-cancer agents include Gleevec (Imatinib Mesylate); Kyprolis (carfilzomib): Velcade (bortezomib); Casodex (bicalutamide); Iressa (gefitinib); alkylating agents such as thiotepa and cyclosphosphamide: alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including altretamine, triethylenemelamine, triethylenephosphoramide, triethiylenethiophosphoramide and trimethylolomelamine; acetogenins (especially bullatacin and bullatacinone); a camptothecin (including the synthetic analogue topotecan); bryostatin; callystatin; CC-1065 (including its adozelesin, carzelesin and bizelesin synthetic analogues); cryptophycins (particularly cryptophycin 1 and cryptophycin 8); dolastatin; duocarmycin (including the synthetic analogues, KW-2189 and CB1-TM1); eleutherobin; pancratistatin; sarcodictyin A; spongistatin; nitrogen mustards such as chlorambucil, chlomaphazine, cholophosphamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, novembichin, phenesterine, prednimustine, trofosfamide, uracil mustard; nitrosureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, and ranimustine; antibiotics such as the enediyne antibiotics (e.g., calicheamicin, such as calicheamicin gammall and calicheamicin omegall (see, e.g., Agnew, Chem. Intl. Ed Engl. 33:183-186 (1994)); dynemicin such as dynemicin A; bisphosphonates such as clodronate; an esperamicin; neocarzinostatin chromophore and related chromoprotein enediyne antiobiotic chromophores, aclacinomysins, actinomycin, authramycin, azaserine, bleomycins, cactinomycin, calicheamicin, carabicin, caminomycin, carminomycin, carzinophilin, chromomycins, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, adriamycin (doxorubicin), morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolino-doxorubicin, deoxydoxorubicin, epirubicin, esorubicin, idarubicin, marcellomycin, mitomycins such as mitomycin C, mycophenolic acid, nogalamycin, olivomycins, peplomycin, potfiromycin, puromycin, quelamycin, rodorubicin, streptonigrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; anti-metabolites such as methotrexate and 5-fluorouracil (5-FU); folic acid analogues such as denopterin, pteropterin, trimetrexate; purine analogs such as fludarabine, 6-mercaptopurine, thiamiprine, thioguanine; pyrimidine analogs such as ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxiflundine, enocitabine, floxuridine; androgens such as calusterone, dromostanolone propionate, epitiostanol, mepitiostane, testolactone; anti-adrenals such as aminoglutethimide, mitotane, trilostane; folic acid replenishers such as frolinic acid; aceglatone; aldophosphamide glycoside; aminolevulinic acid; eniluracil; amsacrine; bestrabucil; bisantrene; edatraxate; defofamine; demecolcine; diaziquone; elfomithine; elliptinium acetate; an epothilone such as epothilone B; etoglucid; gallium nitrate; hydroxyurea; lentinan; lonidamine; maytansinoids such as maytansine and ansamitocins; mitoguazone; mitoxantrone; mopidamol; nitracrine; pentostatin; phenamet; pirarubicin; losoxantrone; podophyllinic acid; 2-ethyihydrazide; procarbazine; PSK polysaccharide complex (JHS Natural Products, Eugene, Oreg.); razoxane; rhizoxin; sizofiran; spirogermanium; tenuazonic acid; triaziquone; 2,2,2-trichlorotriethylamine; trichothecenes such as T-2 toxin, verracurin A, roridin A and anguidine; urethane; vindesine; dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine; arabinoside (Ara-C); cyclophosphamide; thiotepa; taxoids, e.g., Taxol (paclitaxel), Abraxane (cremophor-free, albumin-engineered nanoparticle formulation of paclitaxel), and Taxotere (doxetaxel); chloranbucil; tamoxifen (Nolvadex); raloxifene; aromatase inhibiting 4(5)-imidazoles; 4-hydroxytamoxifen; trioxifene; keoxifene; LY 117018, onapristone; toremifene (Fareston); flutamide, nilutamide, bicalutamide, leuprolide, goserelin; chlorambucil; Gemzar gemcitabine; 6-thioguanine; mercaptopurine; platinum coordination complexes such as cisplatin, oxaliplatin and carboplatin; vinblastine; platinum; etoposide (VP-16); ifosfamide; mitoxantrone; vincristine; Navelbine (vinorelbine); novantrone; teniposide; edatrexate; daunomycin; aminopterin; ibandronate; irinotecan (e.g., CPT-11); topoisomerase inhibitor RFS 2000; difluoromethylomithine (DMFO); retinoids such as retinoic acid; esperamicins; capecitabine (e.g., Xeloda); and pharmaceutically acceptable salts of any of the above.

[0377] Additional non-limiting examples of anti-cancer agents include trastuzumab (Herceptin), bevacizumab (Avastin), cetuximab (Erbitux), rituximab (Rituxan), Taxol, Arimidex, ABVD, avicine, abagovomab, acridine carboxamide, adecatumumab, 17-N-allylamino-17-demethoxygeldanamycin, alpharadin, alvocidib, 3-aminopyridine-2-carboxaldehyde thiosemicarbazone, amonafide, anthracenedione, anti-CD22 immunotoxins, antineoplastics (e.g., cell-cycle nonspecific antineoplastic agents, and other antineoplastics described herein), antitumorigenic herbs, apaziquone, atiprimod, azathioprine, belotecan, bendamustine, BIBW 2992, biricodar, brostallicin, bryostatin, buthionine sulfoximine, CBV (chemotherapy), calyculin, dichloroacetic acid, discodermolide, elsamitrucin, enocitabine, eribulin, exatecan, exisulind, ferruginol, forodesine, fosfestrol, ICE chemotherapy regimen, IT-101, imexon, imiquimod, indolocarbazole, irofulven, laniquidar, larotaxel, lenalidomide, lucanthone, lurtotecan, mafosfamide, mitozolomide, nafoxidine, nedaplatin, olaparib, ortataxel, PAC-1, pawpaw, pixantrone, proteasome inhibitors, rebeccamycin, resiquimod, rubitecan, SN-38, salinosporamide A, sapacitabine, Stanford V, swainsonine, talaporfin, tariquidar, tegafur-uracil, temodar, tesetaxel, triplatin tetranitrate, tris(2-chloroethyl)amine, troxacitabine, uramustine, vadimezan, vinflunine, ZD6126, and zosuquidar.

[0378] Further non-limiting examples of anti-cancer agents include natural products such as vinca alkaloids (e.g., vinblastine, vincristine, and vinorelbine), epidipodophyllotoxins (e.g., etoposide and teniposide), antibiotics (e.g., dactinomycin (actinomycin D), daunorubicin, and idarubicin), anthracyclines, mitoxantrone, bleomycins, plicamycin (mithramycin), mitomycin, enzymes (e.g., L-asparaginase which systemically metabolizes L-asparagine and deprives cells which do not have the capacity to synthesize their own asparagine), antiplatelet agents, antiproliferativelantimitotic alkylating agents such as nitrogen mustards (e.g., mechlorethamine, cyclophosphamide and analogs, melphalan, and chlorambucil), ethylenimines and methylmelamines (e.g., hexaamethylmelaamine and thiotepa), CDK inhibitors (e.g., a CDK 4/6 inhibitor such as palbociclib; seliciclib, UCN-01, P1446A-05, PD-0332991, dinaciclib, P27-00, AT-7519, RGB286638, and SCH727965), alkyl sulfonates (e.g., busulfan), nitrosoureas (e.g., carmustine (BCNU) and analogs, and streptozocin), trazenes-dacarbazinine (DTIC), antiproliferative/antimitotic antimetabolites such as folic acid analogs, pyrimidine analogs (e.g., fluorouracil, floxuridine, and cytarabine), purine analogs and related inhibitors (e.g., mercaptopurine, thioguanine, pentostatin, and 2-chlorodeoxyadenosine), aromatase inhibitors (e.g., anastrozole, exemestane, and letrozole), and platinum coordination complexes (e.g., cisplatin and carboplatin), procarbazine, hydroxyurea, mitotane, aminoglutethimide, histone deacetylase (HDAC) inhibitors (e.g., trichostatin, sodium butyrate, apicidan, suberoyl anilide hydroamic acid, vorinostat, LBH 589, romidepsin, ACY-1215, and panobinostat), mTOR inhibitors (e.g., vistusertib, temsirolimus, everolimus, ridaforolimus, and sirolimus), KSP(Eg5) inhibitors (e.g., Array 520), DNA binding agents (e.g., Zalypsis), PI3K inhibitors such as PI3K delta inhibitor (e.g., GS-1101 and TGR-1202), PI3K delta and gamma inhibitor (e.g., CAL-130), copanlisib, alpelisib and idelalisib; multi-kinase inhibitor (e.g., TG02 and sorafenib), hormones (e.g., estrogen) and hormone agonists such as leutinizing hormone releasing hormone (LHRH) agonists (e.g., goserelin, leuprolide and triptorelin), BAFF-neutralizing antibody (e.g., LY2127399), IKK inhibitors, p38MAPK inhibitors, anti-IL-6 (e.g., CNT0328), telomerase inhibitors (e.g., GRN 163L), aurora kinase inhibitors (e.g., MLN8237), cell surface monoclonal antibodies (e.g., anti-CD38 (HUMAX-CD38), anti-CSI (e.g., elotuzumab), HSP90 inhibitors (e.g., 17 AAG and KOS 953), P13K/Akt inhibitors (e.g., perifosine), Akt inhibitors (e.g., GSK-2141795), PKC inhibitors (e.g., enzastaurin), FTIs (e.g., Zamestra), anti-CD138 (e.g., BT062), Torcl/2 specific kinase inhibitors (e.g., INK128), ER/UPR targeting agents (e.g., MKC-3946), cFMS inhibitors (e.g., ARRY-382), JAK1/2 inhibitors (e.g., CYT387), PARP inhibitors (e.g., olaparib and veliparib (ABT-888)), and BCL-2 antagonists.

[0379] In some embodiments, an anti-cancer agent is selected from mechlorethamine, camptothecin, ifosfamide, tamoxifen, raloxifene, gemcitabine, Navelbine, sorafenib, or any analog or derivative variant of the foregoing.

[0380] In some embodiments, the anti-cancer agent is a HER2 inhibitor. Non-limiting examples of HER2 inhibitors include monoclonal antibodies such as trastuzumab (Herceptin) and pertuzumab (Perjeta); small molecule tyrosine kinase inhibitors such as afatinib, gefitinib (Iressa), erlotinib (Tarceva), pilitinib, CP-654577, CP-724714, canertinib (CI 1033), HKI-272, Iapatinib (GW-572016; Tykerb), PKI-166, AEE788, BMS-599626, HKI-357, BIBW 2992, ARRY-334543, JNJ-26483327, and JNJ-26483327.

[0381] In some embodiments, an anti-cancer agent is an ALK inhibitor. Non-limiting examples of ALK inhibitors include ceritinib, TAE-684 (NVP-TAE694), PF02341066 (crizotinib or 1066), alectinib; brigatinib; entrectinib; ensartinib (X-396); Iorlatinib; ASP3026; CEP-37440; 4SC-203; TL-398; PLB1003; TSR-011; CT-707; TPX-0005, and AP26113. Additional examples of ALK kinase inhibitors are described in examples 3-39 of WO05016894.

[0382] In some embodiments, an anti-cancer agent is an inhibitor of a member downstream of a Receptor Tyrosine Kinase (RTK)/Growth Factor Receptor (e.g., a SHP2 inhibitor (e.g., SHP099, TNO155, RMC-4550, RMC-4630, JAB-3068), a SOS1 inhibitor (e.g., BI-1701963, BI-3406), a Raf inhibitor, a MEK inhibitor, an ERK inhibitor, a PI3K inhibitor, a PTEN inhibitor, an AKT inhibitor, or an mTOR inhibitor (e.g., mTORC1 inhibitor or mTORC2 inhibitor). In some embodiments, the anti-cancer agent is JAB-3312. In some embodiments, an anti-cancer agent is an additional Ras inhibitor (e.g., AMG 510, MRTX1257, MRTX849, JNJ4699157, LY3499446, ARS-3248, or ARS-1620), or a Ras vaccine, or another therapeutic modality designed to directly or indirectly decrease the oncogenic activity of Ras.

[0383] In some embodiments, a therapeutic agent that may be combined with a compound of the present invention is an inhibitor of the MAP kinase (MAPK) pathway (or MAPK inhibitor). MAPK inhibitors include, but are not limited to, one or more MAPK inhibitor described in Cancers (Basel) 2015 September; 7(3): 1758-1784. For example, the MAPK inhibitor may be selected from one or more of trametinib, binimetinib, selumetinib, cobimetinib, LErafAON (NeoPharm), ISIS 5132; vemurafenib, pimasertib, TAK733, RO4987655 (CH4987655); CI-1040; PD-0325901; CH5126766; MAP855; AZD6244; refametinib (RDEA 119/BAY 86-9766); GDC-0973/XL581; AZD8330 (ARRY-424704/ARRY-704); RO5126766 (Roche, described in PLoS One. 2014 Nov. 25; 9(11)); and GSK1120212 (or JTP-74057, described in Clin Cancer Res. 2011 Mar. 1:17(5):989-1000). The MAPK inhibitor may be PLX8394, LXH254, GDC-5573, or LY3009120.

[0384] In some embodiments, an anti-cancer agent is a disrupter or inhibitor of the RAS-RAF-ERK or PI3K-AKT-TOR or PI3K-AKT signaling pathways. The PI3K/AKT inhibitor may include, but is not limited to, one or more PI3K/AKT inhibitor described in Cancers (Basel) 2015 September; 7(3): 1758-1784. For example, the PI3K/AKT inhibitor may be selected from one or more of NVP-BEZ235; BGT226; XL765/SAR245409; SF1126; GDC-0980; PI-103; PF-04691502; PKI-587; GSK2126458.

[0385] In some embodiments, an anti-cancer agent is a PD-1 or PD-L1 antagonist.

[0386] In some embodiments, additional therapeutic agents include ALK inhibitors, HER family inhibitors, EGFR inhibitors, IGF-1R inhibitors, MEK inhibitors, PI3K inhibitors, AKT inhibitors, TOR inhibitors, MCL-1 inhibitors, BCL-2 inhibitors, SHP2 inhibitors, proteasome inhibitors, and immune therapies. In some embodiments, a therapeutic agent may be a pan-RTK inhibitor, such as afatinib.

[0387] IGF-1R inhibitors include linsitinib, or a pharmaceutically acceptable salt thereof.

[0388] EGFR inhibitors include, but are not limited to, small molecule antagonists, antibody inhibitors, or specific antisense nucleotide or siRNA. Useful antibody inhibitors of EGFR include cetuximab (Erbitux), panitumumab (Vectibix), zalutumumab, nimotuzumab, and matuzumab. Further antibody-based EGFR inhibitors include any anti-EGFR antibody or antibody fragment that can partially or completely block EGFR activation by its natural ligand. Non-limiting examples of antibody-based EGFR inhibitors include those described in Modjtahedi et al., Br. J. Cancer 1993, 67:247-253; Teramoto et al., Cancer 1996, 77:639-645; Goldstein et al., Clin. Cancer Res, 1995, 1:1311-1318; Huang et al., 1999, Cancer Res. 15:59(8):1935-40; and Yang et al., Cancer Res. 1999, 59:1236-1243. The EGFR inhibitor can be monoclonal antibody Mab E7.6.3 (Yang, 1999 supra), or Mab C225 (ATCC Accession No. HB-8508), or an antibody or antibody fragment having the binding specificity thereof.

[0389] Small molecule antagonists of EGFR include gefitinib (Iressa), erlotinib (Tarceva), and lapatinib (TykerB). See, e.g., Yan et al., Pharmacogenetics and Pharmacogenomics In Oncology Therapeutic Antibody Development, BioTechniques 2005, 39(4):565-8; and Paez et al., EGFR Mutations In Lung Cancer Correlation With Clinical Response To Gefitinib Therapy, Science 2004, 304(5676):1497-500. Further non-limiting examples of small molecule EGFR inhibitors include any of the EGFR inhibitors described in the following patent publications, and all pharmaceutically acceptable salts of such EGFR inhibitors: EP 0520722; EP 0566226; WO96/33980; U.S. Pat. No. 5,747,498; WO96/30347; EP 0787772; WO97/30034; WO97/30044; WO97/38994; WO97/49688; EP 837063; WO98/02434; WO97/38983; WO95/19774; WO95/19970; WO97/13771; WO98/02437; WO98/02438; WO97/32881; DE 19629652; WO98/33798; WO97/32880; WO97/32880; EP 682027; WO97/02266; WO97/27199; WO98/07726: WO97/34895; WO96/31510; WO98/14449; WO98/14450; WO98/14451; WO95/09847; WO97/19065; WO98/17662; U.S. Pat. Nos. 5,789,427; 5,650,415; 5,656,643; WO99/35146; WO99/35132; WO99/07701; and WO92/20642. Additional non-limiting examples of small molecule EGFR inhibitors include any of the EGFR inhibitors described in Traxier et al., Exp. Opin. Ther. Patents 1998, 8(12):1599-1625. In some embodiments, the therapeutic agent is lapatinib, neratinib, or afatinib.

[0390] MEK inhibitors include, but are not limited to, pimasertib, selumetinib, cobimetinib (Cotellic), trametinib (Mekinist), and binimetinib (Mektovi). In some embodiments, a MEK inhibitor targets a MEK mutation that is a Class I MEK1 mutation selected from D67N; P124L; P124S; and L177V. In some embodiments, the MEK mutation is a Class II MEK1 mutation selected from E51-Q58; F53-Q58; E203K; L177M; C121S; F53L; K57E; Q56P; and K57N.

[0391] PI3K inhibitors include, but are not limited to, wortmannin; 17-hydroxywortmannin analogs described in WO06/044453; 4-[2-(1H-Indazol-4-yl)-6-[[4-(methylsulfonyl)piperazin-1-yl]methyl]thieno[3,2-d]pyrimidin-4-yl]morpholine (also known as pictilisib or GDC-0941 and described in WO09/036082 and WO09/055730); 2-methyl-2-[4-[3-methyl-2-oxo-8-(quinolin-3-yl)-2,3-dihydroimidazo[4,5-c]quinolin-1-yl]phenyl]propionitrile (also known as BEZ 235 or NVP-BEZ 235, and described in WO06/122806); (S)-1-(4-((2-(2-aminopyrimidin-5-yl)-7-methyl-4-morpholinothieno[3,2-d]pyrimidin-6-yl)methyl)piperazin-1-yl)-2-hydroxypropan-1-one (described in WO08/070740); LY294002 (2-(4-morpholinyl)-8-phenyl-4H-I-benzopyran-4-one (available from Axon Medchem); PI 103 hydrochloride (3-[4-(4-morpholinylpyrido-[3,2:4,5]furo[3,2-d]pyrimidin-2-yl] phenol hydrochloride (available from Axon Medchem); PIK 75 (2-methyl-5-nitro-2-[(6-bromoimidazo[1,2-a]pyridin-3-yl)methylene]-1-methylhydrazide-benzenesulfonic acid, monohydrochloride) (available from Axon Medchem); PIK 90 (N-(7,8-dimethoxy-2,3-dihydro-imidazo[1,2-c]quinazolin-5-yl)-nicotinamide (available from Axon Medchem); AS-252424 (5-[I-[5-(4-fluoro-2-hydroxy-phenyl)-furan-2-yl]-meth-(Z)-ylidene]-thiazolidine-2,4-dione (available from Axon Medchem); TGX-221 (7-methyl-2-(4-morpholinyl)-9-[1-(phenylamino)ethyl]-4H-pyrido-[1,2-a]pyrimidin-4-one (available from Axon Medchem); XL-765; and XL-147. Other PI3K inhibitors include demethoxyviridin, perifosine, CAL101, PX-866, BEZ235, SF1126, INK1117, IPI-145, BKM120, XL147, XL765, Palomid 529, GSK1059615, ZSTK474, PWT33597, IC87114, TGI 00-115, CAL263, PI-103, GNE-477, CUDC-907, and AEZS-136.

[0392] AKT inhibitors include, but are not limited to, Akt-1-1 (inhibits Aktl) (Bamett et al., Biochem. J. 2005, 385(Pt. 2): 399-408); Akt-1-1,2 (inhibits Akl and 2) (Bamett et al., Biochem. J. 2005, 385(Pt. 2): 399-408); API-59CJ-Ome (e.g., Jin et al., Br. J. Cancer 2004, 91:1808-12); 1-H-imidazo[4,5-c]pyridinyl compounds (e.g., WO 05/011700); indole-3-carbinol and derivatives thereof (e.g., U.S. Pat. No. 6,656,963; Sarkar and Li J Nutr. 2004, 134(12 Suppl):3493S-3498S); perifosine (e.g., interferes with Akt membrane localization; Dasmahapatra et al. Clin. Cancer Res. 2004, 10(15):5242-52); phosphatidylinositol ether lipid analogues (e.g., Gills and Dennis Expert. Opin. Investig. Drugs 2004, 13:787-97); and triciribine (TCN or API-2 or NCI identifier: NSC 154020; Yang et al., Cancer Res. 2004, 64:4394-9).

[0393] mTOR inhibitors include, but are not limited to, ATP-competitive mTORC1/mTORC2 inhibitors, e.g., PI-103, PP242, PP30; Torin 1; FKBP12 enhancers; 4H-1-benzopyran-4-one derivatives; and rapamycin (also known as sirolimus) and derivatives thereof, including: temsirolimus (Torisel); everolimus (Afinitor; WO94/09010): ridaforolimus (also known as deforolimus or AP23573); rapalogs, e.g., as disclosed in WO98/02441 and WO01/14387, e.g. AP23464 and AP23841; 40-(2-hydroxyethyl)rapamycin; 40-[3-hydroxy(hydroxymethyl)methylpropanoate]-rapamycin (also known as CC1779); 40-epi-(tetrazolyt)-rapamycin (also called ABT578); 32-deoxorapamycin; 16-pentynyloxy-32(S)-dihydrorapanycin: derivatives disclosed in WO05/005434; derivatives disclosed in U.S. Pat. Nos. 5,258,389, 5,118,677, 5,118,678, 5,100,883, 5,151,413, 5,120,842, and 5,256,790, and in WO94/090101. WO92/05179. WO93/111130, WO94/02136. WO94/02485, WO95/14023, WO94/02136, WO95/16691, WO96/41807, WO96/41807, and WO2018204416; and phosphorus-containing rapamycin derivatives (e.g., WO05/016252). In some embodiments, the mTOR inhibitor is a bisteric inhibitor, such as RMC-5552.

[0394] BRAF inhibitors that may be used in combination with compounds of the invention include, for example, vemurafenib, dabrafenib, and encorafenib. A BRAF may comprise a Class 3 BRAF mutation. In some embodiments, the Class 3 BRAF mutation is selected from one or more of the following amino acid substitutions in human BRAF: D287H; P367R; V459L; G466V; G466E; G466A; S467L; G469E; N581S; N581I; D594N; D594G; D594A; D594H; F595L; G596D; G596R and A762E.

[0395] MCL-1 inhibitors include, but are not limited to, AMG-176, MIK665, and S63845. The myeloid cell leukemia-1 (MCL-1) protein is one of the key anti-apoptotic members of the B-cell lymphoma-2 (BCL-2) protein family. Over-expression of MCL-1 has been closely related to tumor progression as well as to resistance, not only to traditional chemotherapies but also to targeted therapeutics including BCL-2 inhibitors such as ABT-263.

[0396] In some embodiments, the additional therapeutic agent is selected from the group consisting of a HER2 family inhibitor, a SHP2 inhibitor, CDK4/6 inhibitor, an mTOR inhibitor, a SOS1 inhibitor, or a PD-L1 inhibitor. See, e.g., Hallin et al., Cancer Discovery, DOI: 10.1158/2159-8290 (Oct. 28, 2019) and Canon et al., Nature, 575:217(2019).

[0397] Proteasome inhibitors include, but are not limited to, carfilzomib (Kyprolis), bortezomib (Velcade), and oprozomib.

[0398] Immune therapies include, but are not limited to, monoclonal antibodies, immunomodulatory imides (IMiDs), GITR agonists, genetically engineered T-cells (e.g., CAR-T cells), bispecific antibodies (e.g., BiTEs), and anti-PD-1, anti-PDL-1, anti-CTLA4, anti-LAGI, and anti-OX40 agents).

[0399] Immunomodulatory agents (IMiDs) are a class of immunomodulatory drugs (drugs that adjust immune responses) containing an imide group. The IMiD class includes thalidomide and its analogues (lenalidomide, pomalidomide, and apremilast).

[0400] Exemplary anti-PD-1 antibodies and methods for their use are described by Goldberg et al., Blood 2007, 110(1):186-192; Thompson et al., Clin. Cancer Res. 2007, 13(6):1757-1761; and WO06/121168 A1), as well as described elsewhere herein.

[0401] GITR agonists include, but are not limited to, GITR fusion proteins and anti-GITR antibodies (e.g., bivalent anti-GITR antibodies), such as, a GITR fusion protein described in U.S. Pat. No. 6,111,090, , U.S. Pat. No. 8,586,023, WO2010/003118 and WO2011/090754; or an anti-GITR antibody described, e.g., in U.S. Pat. No. 7,025,962, EP 1947183, U.S. Pat. Nos. 7,812,135, 8,388,967, 8,591,886. U.S. Pat. No. 7,618,632, EP 1866339, and WO2011/028683, WO2013/039954, WO05/007190, WO07/133822, WO05/055808, WO99/40196, WO01/03720, WO99/20758, WO06/083289, WO05/115451, and WO2011/051726.

[0402] Another example of a therapeutic agent that may be used in combination with the compounds of the invention is an anti-angiogenic agent. Anti-angiogenic agents are inclusive of, but not limited to, in vitro synthetically prepared chemical compositions, antibodies, antigen binding regions, radionuclides, and combinations and conjugates thereof. An anti-angiogenic agent can be an agonist, antagonist, allosteric modulator, toxin or, more generally, may act to inhibit or stimulate its target (e.g., receptor or enzyme activation or inhibition), and thereby promote cell death or arrest cell growth. In some embodiments, the one or more additional therapies include an anti-angiogenic agent.

[0403] Anti-angiogenic agents can be MMP-2 (matrix-metalloproteinase 2) inhibitors, MMP-9 (matrix-metalloprotienase 9) inhibitors, and COX-II (cyclooxygenase 11) inhibitors. Non-limiting examples of anti-angiogenic agents include rapamycin, temsirolimus (CCI-779), everolimus (RAD001), sorafenib, sunitinib, and bevacizumab. Examples of useful COX-II inhibitors include alecoxib, valdecoxib, and rofecoxib. Examples of useful matrix metalloproteinase inhibitors are described in WO96/33172, WO96/27583, WO98/07697, WO98/03516, WO98/34918, WO98/34915, WO98/33768, WO98/30566, WO90/05719, WO99/52910, WO99/52889, WO99/29667, WO99007675, EP0606046, EP0780386, EP1786785, EP1181017, EP0818442, EP1004578, and US20090012085, and U.S. Pat. Nos. 5,863,949 and 5,861,510. Preferred MMP-2 and MMP-9 inhibitors are those that have little or no activity inhibiting MMP-1. More preferred, are those that selectively inhibit MMP-2 or AMP-9 relative to the other matrix-metalloproteinases (i.e., MAP-1, MMP-3, MMP-4, MMP-5, MMP-6, MMP-7, MMP-8, MMP-10, MMP-11, MMP-12, and MMP-13). Some specific examples of MMP inhibitors are AG-3340, RO 32-3555, and RS 13-0830.

[0404] Further exemplary anti-angiogenic agents include KDR (kinase domain receptor) inhibitory agents (e.g., antibodies and antigen binding regions that specifically bind to the kinase domain receptor), anti-VEGF agents (e.g., antibodies or antigen binding regions that specifically bind VEGF (such as bevacizumab), or soluble VEGF receptors or a ligand binding region thereof) such as VEGF-TRAP, and anti-VEGF receptor agents (e.g., antibodies or antigen binding regions that specifically bind thereto). EGFR inhibitory agents (e.g., antibodies or antigen binding regions that specifically bind thereto) such as Vectibix (panitumumab), erlotinib (Tarceva), anti-Angl and anti-Ang2 agents (e.g., antibodies or antigen binding regions specifically binding thereto or to their receptors, e.g., Tie2/Tek), and anti-Tie2 kinase inhibitory agents (e.g., antibodies or antigen binding regions that specifically bind thereto). Other anti-angiogenic agents include Campath, IL-8, B-FGF, Tek antagonists (US2003/0162712; U.S. Pat. No. 6,413,932), anti-TWEAK agents (e.g., specifically binding antibodies or antigen binding regions, or soluble TWEAK receptor antagonists; see U.S. Pat. No. 6,727,225), ADAM distintegrin domain to antagonize the binding of integrin to its ligands (US 2002/0042368), specifically binding anti-eph receptor and/or anti-ephrin antibodies or antigen binding regions (U.S. Pat. Nos. 5,981,245; 5,728,813; 5,969,110; 6,596,852; 6,232,447; 6,057,124 and patent family members thereof), and anti-PDGF-BB antagonists (e.g., specifically binding antibodies or antigen binding regions) as well as antibodies or antigen binding regions specifically binding to PDGF-BB ligands, and PDGFR kinase inhibitory agents (e.g., antibodies or antigen binding regions that specifically bind thereto). Additional anti-angiogenic agents include: SD-7784 (Pfizer, USA); cilengitide (Merck KGaA, Germany, EPO 0770622); pegaptanib octasodium, (Gilead Sciences, USA); Alphastatin, (BioActa, UK); M-PGA, (Celgene, USA, U.S. Pat. No. 5,712,291); ilomastat, (Arriva, USA, U.S. Pat. No. 5,892,112); emaxanib, (Pfizer, USA, U.S. Pat. No. 5,792,783); vatalanib, (Novartis, Switzerland); 2-methoxyestradiol (EntreMed, USA); TLC ELL-12 (Elan, Ireland); anecortave acetate (Alcon, USA); alpha-D148 Mab (Amgen, USA); CEP-7055 (Cephalon, USA); anti-Vn Mab (Crucell, Netherlands), DACantiangiogenic (ConjuChem, Canada); Angiocidin (InKine Pharmaceutical, USA); KM-2550 (Kyowa Hakko, Japan); SU-0879 (Pfizer, USA); CGP-79787 (Novartis, Switzerland, EP 0970070); ARGENT technology (Ariad, USA); YIGSR-Stealth (Johnson & Johnson, USA); fibrinogen-E fragment (BioActa, UK); angiogenic inhibitor (Trigen, UK); TBC-1635 (Encysive Pharmaceuticals, USA); SC-236 (Pfizer, USA); ABT-567 (Abbott, USA); Metastatin (EntreMed, USA); maspin (Sosei, Japan); 2-methoxyestradiol (Oncology Sciences Corporation, USA); ER-68203-00 (IV AX, USA); BeneFin (Lane Labs, USA); Tz-93 (Tsumura, Japan); TAN-1120 (Takeda, Japan); FR-111142 (Fujisawa, Japan, JP 02233610); platelet factor 4 (RepliGen, USA, EP 407122); vascular endothelial growth factor antagonist (Borean, Denmark); bevacizumab (pINN) (Genentech, USA); angiogenic inhibitors (SUGEN, USA); XL 784 (Exelixis, USA); XL 647 (Exelixis, USA); MAb, alpha5beta3 integrin, second generation (Applied Molecular Evolution, USA and MedImmune, USA); enzastaurin hydrochloride (Lilly, USA); CEP 7055 (Cephalon, USA and Sanofi-Synthelabo, France); BC 1 (Genoa Institute of Cancer Research, Italy); rBPI 21 and BPI-derived antiangiogenic (XOMA, USA); PI 88 (Progen, Australia); cilengitide (Merck KGaA, German; Munich Technical University, Germany, Scripps Clinic and Research Foundation, USA); AVE 8062 (Ajinomoto, Japan); AS 1404 (Cancer Research Laboratory, New Zealand); SG 292, (Telios, USA); Endostatin (Boston Childrens Hospital, USA); ATN 161 (Attenuon, USA); 2-methoxyestradiol (Boston Childrens Hospital, USA); ZD 6474, (AstraZeneca, UK); ZD 6126, (Angiogene Pharmaceuticals, UK); PPI 2458, (Praecis, USA); AZD 9935, (AstraZeneca, UK); AZD 2171, (AstraZeneca, UK); vatalanib (pINN), (Novartis, Switzerland and Schering AG, Germany); tissue factor pathway inhibitors, (EntreMed, USA); pegaptanib (Pinn), (Gilead Sciences, USA); xanthorrhizol, (Yonsei University, South Korea); vaccine, gene-based, VEGF-2, (Scripps Clinic and Research Foundation, USA); SPV5.2, (Supratek, Canada); SDX 103, (University of California at San Diego, USA); PX 478, (ProIX, USA); METASTATIN, (EntreMed, USA); troponin I, (Harvard University, USA); SU 6668, (SUGEN, USA); OXI 4503, (OXiGENE, USA); o-guanidines, (Dimensional Pharmaceuticals, USA); motuporamine C, (British Columbia University, Canada); CDP 791, (Celltech Group, UK); atiprimod (pINN), (GlaxoSmithKline, UK); E 7820. (Eisai. Japan); CYC 381, (Harvard University, USA); AE 941, (Aetema, Canada); vaccine, angiogenic, (EntreMed, USA); urokinase plasminogen activator inhibitor, (Dendreon, USA); oglufanide (pINN), (Melmotte, USA); HIF-lalfa inhibitors, (Xenova, UK); CEP 5214. (Cephalon, USA); BAY RES 2622, (Bayer, Germany); Angiocidin, (InKine, USA); A6, (Angstrom, USA); KR 31372, (Korea Research Institute of Chemical Technology, South Korea): GW 2286, (GlaxoSmithKline. UK); EHT 0101, (ExonHit, France); CP 868596, (Pfizer, USA); CP 564959, (OSI, USA); CP 547632, (Pfizer, USA); 786034, (GlaxoSmithKline, UK): KRN 633, (Kirin Brewery, Japan); drug delivery system, intraocular, 2-methoxyestradiol; anginex (Maastricht University, Netherlands, and Minnesota University, USA); ABT 510 (Abbott, USA); AAL 993 (Novartis, Switzerland); VEGI (ProteomTech, USA); tumor necrosis factor-alpha inhibitors; SU 11248 (Pfizer, USA and SUGEN USA); ABT 518, (Abbott, USA); YH16 (Yantai Rongchang, China); S-3APG (Boston Childrens Hospital, USA and EntreMed, USA); MAb, KDR (ImClone Systems, USA); MAb, alpha5 beta (Protein Design, USA); KDR kinase inhibitor (Celltech Group, UK, and Johnson & Johnson, USA); GFB 116 (South Florida University, USA and Yale University, USA); CS 706 (Sankyo, Japan); combretastatin A4 prodrug (Arizona State University, USA); chondroitinase AC (IBEX, Canada); BAY RES 2690 (Bayer, Germany); AGM 1470 (Harvard University, USA, Takeda, Japan, and TAP, USA); AG 13925 (Agouron, USA); Tetrathiomolybdate (University of Michigan, USA); GCS 100 (Wayne State University, USA) CV 247 (Ivy Medical, UK); CKD 732 (Chong Kun Dang, South Korea); irsogladine, (Nippon Shinyaku, Japan); RG 13577 (Aventis, France); WX 360 (Wilex, Germany); squalamine, (Genaera, USA); RPI 4610 (Sima, USA); heparanase inhibitors (InSight, Israel); KL 3106 (Kolon, South Korea); Honokiol (Emory University, USA); ZK CDK (Schering AG, Germany); ZK Angio (Schering AG, Germany); ZK 229561 (Novartis, Switzerland, and Schering AG, Germany); XMP 300 (XOMA, USA); VGA 1102 (Taisho, Japan); VE-cadherin-2 antagonists(ImClone Systems, USA); Vasostatin (National Institutes of Health, USA); FIk-1 (ImClone Systems, USA); TZ 93 (Tsumura, Japan); TumStatin (Beth Israel Hospital, USA); truncated soluble FLT 1 (vascular endothelial growth factor receptor 1) (Merck & Co, USA); Tie-2 ligands (Regeneron, USA); and thrombospondin 1 inhibitor (Allegheny Health, Education and Research Foundation, USA).

[0405] Further examples of therapeutic agents that may be used in combination with compounds of the invention include agents (e.g., antibodies, antigen binding regions, or soluble receptors) that specifically bind and inhibit the activity of growth factors, such as antagonists of hepatocyte growth factor (HGF, also known as Scatter Factor), and antibodies or antigen binding regions that specifically bind its receptor, c-Met.

[0406] Another example of a therapeutic agent that may be used in combination with compounds of the invention is an autophagy inhibitor. Autophagy inhibitors include, but are not limited to chloroquine, 3-methyladenine, hydroxychloroquine (Plaquenil), bafilomycin A1, 5-amino-4-imidazole carboxamide riboside (AICAR), okadaic acid, autophagy-suppressive algal toxins which inhibit protein phosphatases of type 2A or type 1, analogues of cAMP, and drugs which elevate cAMP levels such as adenosine, LY204002, N6-mercaptopurine riboside, and vinblastine. In addition, antisense or siRNA that inhibits expression of proteins including but not limited to ATG5 (which are implicated in autophagy), may also be used. In some embodiments, the one or more additional therapies include an autophagy inhibitor.

[0407] Another example of a therapeutic agent that may be used in combination with compounds of the invention is an anti-neoplastic agent. In some embodiments, the one or more additional therapies include an anti-neoplastic agent. Non-limiting examples of anti-neoplastic agents include acemannan, aclarubicin, aldesleukin, alemtuzumab, alitretinoin, altretamine, amifostine, aminolevulinic acid, amrubicin, amsacrine, anagrelide, anastrozole, ancer, ancestim, arglabin, arsenic trioxide, BAM-002 (Novelos), bexarotene, bicalutamide, broxuridine, capecitabine, celmoleukin, cetrorelix, dadribine, clotrimazole, cytarabine ocfosfate, DA 3030 (Dong-A), daclizumab, denileukin diftitox, deslorelin, dexrazoxane, dilazep, docetaxel, docosanol, doxercalciferol, doxifluridine, doxorubicin, bromocriptine, carmustine, cytarabine, fluorouracil, HIT diclofenac, interferon alfa, daunorubicin, doxorubicin, tretinoin, edelfosine, edrecolomab, eflomithine, emitefur, epirubicin, epoetin beta, etoposide phosphate, exemestane, exisulind, fadrozole, filgrastim, finasteride, fludarabine phosphate, formestane, fotemustine, gallium nitrate, gemcitabine, gemtuzumab zogamicin, gimeraciloteracil/tegafur combination, glycopine, goserelin, heptaplatin, human chorionic gonadotropin, human fetal alpha fetoprotein, ibandronic acid, idarubicin, (imiquimod, interferon alfa, interferon alfa, natural, interferon alfa-2, interferon alfa-2a, interferon alfa-2b, interferon alfa-NI, interferon alfa-n3, interferon alfacon-1, interferon alpha, natural, interferon beta, interferon beta-Ia, interferon beta-Ib, interferon gamma, natural interferon gamma-Ia, interferon gamma-Ib, interleukin-1 beta, iobenguane, irinotecan, irsogladine, lanreotide, LC 9018 (Yakult), leflunomide, lenograstim, lentinan sulfate, letrozole, leukocyte alpha interferon, leuprorelin, levamisole+fluorouracil, liarozole, lobaplatin, lonidamine, lovastatin, masoprocol, melarsoprol, metoclopramide, mifepristone, miltefosine, mirimostim, mismatched double stranded RNA, mitoguazone, mitolactol, mitoxantrone, molgramostim, nafarelin, naloxone+pentazocine, nartograstim, nedaplatin, nilutamide, noscapine, novel erythropoiesis stimulating protein, NSC 631570 octreotide, oprelvekin, osaterone, oxaliplatin, paclitaxel, pamidronic acid, pegaspargase, peginterferon alfa-2b, pentosan polysulfate sodium, pentostatin, picibanil, pirarubicin, rabbit antithymocyte polyclonal antibody, polyethylene glycol interferon alfa-2a, porfimer sodium, raloxifene, raltitrexed, rasburiembodiment, rhenium Re 186 etidronate, RII retinamide, rituximab, romurtide, samarium (153 Sm) lexidronam, sargramostim, sizofiran, sobuzoxane, sonermin, strontium-89 chloride, suramin, tasonermin, tazarotene, tegafur, temoporfin, temozolomide, teniposide, tetrachlorodecaoxide, thalidomide, thymalfasin, thyrotropin alfa, topotecan, toremifene, tositumomab-iodine 131, trastuzumab, treosulfan, tretinoin, trilostane, trimetrexate, triptorelin, tumor necrosis factor alpha, natural, ubenimex, bladder cancer vaccine, Maruyama vaccine, melanoma lysate vaccine, valrubicin, verteporfin, vinorelbine, virulizin, zinostatin stimalamer, or zoledronic acid: abarelix; AE 941 (Aetema), ambamustine, antisense oligonucleotide, bcl-2 (Genta), APC 8015 (Dendreon), decitabine, dexaminoglutethimide, diaziquone, EL 532 (Elan), EM 800 (Endorecherche), eniluracil, etanidazole, fenretinide, filgrastim SD01 (Amgen), fulvestrant, galocitabine, gastrin 17 immunogen, HLA-B7 gene therapy (Vical), granulocyte macrophage colony stimulating factor, histamine dihydrochloride, ibritumomab tiuxetan, ilomastat, IM 862 (Cytran), interleukin-2, iproxifene, LDI 200 (Milkhaus), leridistim, lintuzumab, CA 125 MAb (Biomira), cancer MAb (Japan Pharmaceutical Development), HER-2 and Fc MAb (Medarex), idiotypic 105AD7 MAb (CRC Technology), idiotypic CEA MAb (Trilex), LYM-1-iodine 131 MAb (Techni clone), polymorphic epithelial mucin-yttrium 90 MAb (Antisoma), marimastat, menogaril, mitumomab, motexafin gadolinium, MX 6 (Galderma), nelarabine, nolatrexed, P 30 protein, pegvisomant, pemetrexed, porfiromycin, prinomastat, RL 0903 (Shire), rubitecan, satraplatin, sodium phenylacetate, sparfosic acid, SRL 172 (SR Pharma), SU 5416 (SUGEN), TA 077 (Tanabe), tetrathiomolybdate, thaliblastine, thrombopoietin, tin ethyl etiopurpurin, tirapazamine, cancer vaccine (Biomira), melanoma vaccine (New York University), melanoma vaccine (Sloan Kettering Institute), melanoma oncolysate vaccine (New York Medical College), viral melanoma cell lysates vaccine (Royal Newcastle Hospital), or valspodar.

[0408] Additional examples of therapeutic agents that may be used in combination with compounds of the invention include ipilimumab (Yervoy); tremelimumab; galiximab; nivolumab, also known as BMS-936558 (Opdivo); pembrolizumab (Keytruda); avelumab (Bavencio); AMP224; BMS-936559; MPDL3280A, also known as RG7446; MEDI-570; AMG557; MGA271; IMP321; BMS-663513; PF-05082566; CDX-1127; anti-OX40 (Providence Health Services); huMAbOX40L; atacicept; CP-870893; lucatumumab; dacetuzumab; muromonab-CD3; ipilumumab; MED14736 (Imfinzi); MSB0010718C; AMP 224; adalimumab (Humira); ado-trastuzumab emtansine (Kadcyla); aflibercept (Eylea); alemtuzumab (Campath); basiliximab (Simulect); belimumab (Benlysta); basiliximab (Simulect); belimumab (Benlysta); brentuximab vedotin (Adcetris); canakinumab (Ilaris); certolizumab pegol (Cimzia); daclizumab (Zenapax); daratumumab (Darzalex); denosumab (Prolia); eculizumab (Soliris); efalizumab (Raptiva); gemtuzumab ozogamicin (Mylotarg); golimumab (Simponi); ibritumomab tiuxetan (Zevalin); infliximab (Remicade); motavizumab (Numax); natalizumab (Tysabri); obinutuzumab (Gazyva); ofatumumab (Arzerral); omalizumab (Xolair); palivizumab (Synagis); pertuzumab (Perjeta); pertuzumab (Perjeta); ranibizumab (Lucentis); raxibacumab (Abthrax); tocilizumab (Actemra); tositumomab; tositumomab-i-131; tositumomab and tositumomab-i-131 (Bexxar); ustekinumab (Stelara); AMG 102; AMG 386; AMG 479; AMG 655; AMG 706; AMG 745; and AMG 951.

[0409] The compounds described herein can be used in combination with the agents disclosed herein or other suitable agents, depending on the condition being treated. Hence, in some embodiments the one or more compounds of the disclosure will be co-administered with other therapies as described herein. When used in combination therapy, the compounds described herein may be administered with the second agent simultaneously or separately. This administration in combination can include simultaneous administration of the two agents in the same dosage form, simultaneous administration in separate dosage forms, and separate administration. That is, a compound described herein and any of the agents described herein can be formulated together in the same dosage form and administered simultaneously. Alternatively, a compound of the invention and any of the therapies described herein can be simultaneously administered, wherein both the agents are present in separate formulations. In another alternative, a compound of the present disclosure can be administered and followed by any of the therapies described herein, or vice versa. In some embodiments of the separate administration protocol, a compound of the invention and any of the therapies described herein are administered a few minutes apart, or a few hours apart, or a few days apart.

[0410] In some embodiments of any of the methods described herein, the first therapy (e.g., a compound of the invention) and one or more additional therapies are administered simultaneously or sequentially, in either order. The first therapeutic agent may be administered immediately, up to 1 hour, up to 2 hours, up to 3 hours, up to 4 hours, up to 5 hours, up to 6 hours, up to 7 hours, up to, 8 hours, up to 9 hours, up to hours, up to 11 hours, up to 12 hours, up to 13 hours, 14 hours, up to hours 16, up to 17 hours, up 18 hours, up to 19 hours up to 20 hours, up to 21 hours, up to 22 hours, up to 23 hours, up to 24 hours, or up to 1-7, 1-14, 1-21 or 1-30 days before or after the one or more additional therapies.

EXAMPLES

Materials and Methods

[0411] In some aspects, the invention includes the intermediates, examples, and synthetic methods described herein in all of their embodiments.

[0412] The compounds of the Formula I may be prepared by the methods described below, together with synthetic methods known in the art of organic chemistry, or modifications and derivatizations that are familiar to those of ordinary skill in the art. The starting materials used herein are commercially available or may be prepared by routine methods known in the art, e.g., methods disclosed in standard reference books such as the Compendium of Organic Synthetic Methods, Vol. I-VI (Wiley-Interscience); or the Comprehensive Organic Transformations, by R.C. Larock (Wiley-Interscience). Preferred methods include, but are not limited to, those described below.

[0413] During any of the following synthetic sequences it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned. This can be achieved by means of conventional protecting groups, such as those described in T.W. Greene, Protective Groups in Organic Chemistry, John Wiley & Sons (1981); T.W. Greene and P.G.M. Wuts, Protective Groups in Organic Chemistry, John Wiley & Sons (1991), T.W. Greene and P.G.M. Wuts, Protective Groups in Organic Chemistry, John Wiley & Sons (1999); T.W. Greene and P.G.M. Wuts, Protective Groups in Organic Chemistry, John Wiley & Sons (2006); and T.W. Greene and P.G.M. Wuts, Protective Groups in Organic Chemistry, John Wiley & Sons (2014), which are hereby incorporated by reference in their entireties.

[0414] Compounds of Formula I, or their pharmaceutical acceptable salts, and the intermediates used in the synthesis of the compounds of this invention can be prepared according to the reaction schemes discussed hereinbelow and the general skill in the art.

[0415] Unless otherwise indicated, the substituents in the schemes are defined as above. Isolation and purification of the products is accomplished by standard procedures, which are known to a chemist of ordinary skill.

[0416] When a general or exemplary synthetic procedure is referred to, one skilled in the art can readily determine the appropriate reagents, if not indicated, extrapolating from the general or exemplary procedures. Some of the general procedures are given as examples for preparing specific compounds. One skilled in the art can readily adapt such procedures to the synthesis of other compounds. Representation of an unsubstituted position in structures shown or referred to in the general procedures is for convenience and does not preclude substitution as described elsewhere herein. For specific groups that can be present, either as R groups in the general procedures or as optional substituents not shown, refer to the descriptions in the remainder of this document, including the claims, summary and detailed description.

[0417] The process to produce compounds of the present invention is preferably carried out at about atmospheric pressure although higher or lower pressures can be used if desired. Substantially equimolar amounts of reactants are preferably used although higher or lower amounts may also be used.

[0418] Unless otherwise noted, all materials and reagents were obtained from commercial suppliers and used without further purification. Reactions were monitored by thin layer chromatography (TLC) on silica gel 60 F254 (0.2 mm) precoated aluminum foil or glass-backed and visualized using UV light or appropriate TLC stains. Flash chromatography was performed using either an Agela Technologies CombiFlash with CHEETAH Purification System or an ISCO CombiFlash Rf 200 Organic Purification System. Preparative TLC was performed on Xinnuo Silica Gel 10-40 m size 2020 cm plates with a thickness of 1000 m or equivalent.

[0419] .sup.1H NMR (300 or 400 MHz) spectra were recorded on Bruker or Varian instruments at room temperature with TMS or the residual solvent peak as the internal standard. The line positions or multiples are given in () and the coupling constants (J) are given as absolute values in Hertz (Hz). The multiplicities in .sup.1H NMR spectra are abbreviated as follows: (singlet), d (doublet), t (triplet), q (quartet), quint (quintet), m (multiplet), mc (centered multip-let), br or broad (broadened).

[0420] NMR data are generally collected in deuterated solvents such as DMSO-d.sub.6, CD3OD, CDCl3 or Acetonitrile-d.sub.3, although the deuterated status of the solvent may or may not be explicitly shown in NMR data section.

[0421] Preparative HPLC purifications were performed on a Waters Mass-Directed Purification System equipped with 2545 or 2525 Binary Gradient Module, 2767 Sample Manager, a Column Fluidics Organizer (CFO), 2489 Photodiode Array Detector, a 515 pump for makeup flow, a reagent manager, a 515 pump for at-column-dilution, Zspray single-quadrupole Mass Detector equipped with a Z-spray electrospray interface, controlled by MassLynx Version 4.1 with FractionLynx software. The mobile phases were water and acetonitrile with 0.1% formic acid or 0.01 M NH.sub.4HCO.sub.3 unless otherwise noted. The flow rate was 25 mL/min. After the columns, a 1:1000 LC packings flow splitter allowed transfer of a small portion of the eluent into the UV detector and, subsequently, a 10% portion into the ZQ MS. The electrospray source was set at 3.0 kV capillary voltage, 30 V cone voltage, 110 C. source temperature, 350 C. desolvation temperature, 600 L/h desolvation gas flow, and 60 L/h cone gas flow. For the analyzer, the multiplier was set at 550 for preparative tune method.

[0422] Analytical LCMS data was collected on LCMS01, LCMS02, UPLC01, or UPLC02 instruments with a mobile phase of acetonitrile (B) and HPLC grade water (A) with either 0.05% formic acid or 0.05% TFA in HPLC grade water (B) unless otherwise noted.

[0423] LCMS01 is a Shimadzu LC-20ADXR HPLC equipped with a SPD-M20A detector and LCMS-2020 for ionization. The system uses the following conditions for either 5 or 3 minute run time.

[0424] 5 minute run: Ascentis Express C18 column, 2 m, 3.050 mm. The flow rate is 1.5 mL/min, the run time is 5 min, and the gradient profiles are 0.01 min 5% B, 3.00 min 100% B, 4.60 min 100% B, 4.90 min 5% B, 5.00 min 0% B. The LCMS-2020 instrument utilized electrospray ionization in positive (ES+) or negative (ES) mode.

[0425] 3 minute run: Ascentis Express C18 column, 2 m, 3.050 mm. The flow rate is 1.5 mL/min, the run time is 3 min, and the gradient profiles are 0.01 min 5% B, 2.00 min 100% B, 2.70 min 100% B, 2.75 min 5% B, 3.00 min 0% B.

[0426] Agilent LCMS is an Agilent 1260 HPLC equipped with 6120/6125 single-quadrupole Mass detector, ESI for ionization. The system uses the following conditions for 2.5 min run time.

[0427] Conditions: Waters CORTECS C18+ column, 2.7 m, 4.630 mm. The flow rate is 1.8 mL/min, the run time is 2.5 min, and the gradient profiles are 0.00 min 5% B, 1.00 min 95% B, 2.0 min 95% B, 2.1 min 5% B, 2.5 min 5% B. Premier XE MS utilized electrospray ionization in positive (ES+) or negative (ES) modes.

[0428] UPLC01 is an Agilent Technologies 1260 Infinity II attached to a DAD (G4212-60008) detector. Waters T3 column, 4.6100 mm was heated to 60 C. with detection at 254 nm and at 220 nm and electrospray ionization in positive mode was used. Table 2 below lists the mobile phase gradient (solvent A: 0.05% TFA in water, solvent B: 0.05% TFA in acetonitrile) and flow rate for the analytical UPLC program.

TABLE-US-00002 TABLE 2 Time (mm) A % B % Flow Rate mL/mm 0.00 95.0 5.0 1.00 8.00 5.0 95.0 1.00 10.00 5.0 95.0 1.00 11.00 95.0 5.0 1.00 13.00 95.0 5.0 1.00

[0429] UPLC02 is an ACQUITY sample manager attached to PDA detector. ACQUITYUPLC BEH CI8 1.7 m 2.150 mm was heated to 45 C. with detection at 254/214 nm. Table 3 below lists the mobile phase gradient (solvent A: 0.05% TFA in water, solvent B: 0.05% TFA in acetonitrile) and flow rate for the analytical UPLC program.

TABLE-US-00003 TABLE 3 Time (mm) A % B % Flow Rate (mL/mm) 0.00 95 5 0.50 2.00 5 95 0.50 2.50 5 95 0.50 2.70 95 5 0.50 3.50 95 5 0.50

Example 1Synthesis of Intermediates

A. Methyl (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoyl)hexahydropyridazine-3-carboxylate

[0430] ##STR00144##

[0431] Step A

[0432] To a solution of (S)-methyl 2-(tert-butoxycarbonylamino)-3-(3-hydroxyphenyl)propanoate (10.0 g, 33.9 mmol) in dichloromethane (100 mL) was added imidazole (4.6 g, 67.8 mmol) and TIPSCl (7.8 g, 40.7 mmol). The mixture was stirred for 16 hours and then diluted with dichloromethane (200 mL) and washed with H.sub.2O (3150 mL). The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated to give a residue that was purified by silica gel chromatography (0-10% ethyl acetate in petroleum ether) to give methyl (S)-2-((tert-butoxycarbonyl)amino)-3-(3-((triisopropylsilyl)oxy)phenyl)propanoate (98% yield) as a colorless oil. ESI-MS m/z=474.2 [M+Na].sup.+

[0433] Step B

[0434] Methyl (S)-2-((tert-butoxycarbonyl)amino)-3-(3-((triisopropylsilyl)oxy)phenyl)propanoate (7.5 g, 16.6 mmol), bis(pinacolato)diborane (6.3 g, 24.9 mmol), [Ir(OMe)(COD)]2 (1.1 g, 1.66 mmol) and 4-tert-butyl-2-(4-tert-butyl-2-pyridyl)pyridine (1.3 g, 4.98 mmol) were combined in flask. After purging with argon, tetrahydrofuran (75 mL) was added. The flask was sealed, heated to 80 C. and stirred for 16 hours. The mixture was concentrated in vacuo and then purified by silica gel chromatography (0-20% ethyl acetate in petroleum ether) to give methyl (S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoate (78% yield) as a white solid. ESI-MS m/z=600.4 [M+Na].sup.+.

[0435] Step C

[0436] To a solution of methyl (S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoate (4.95 g, 6.88 mmol) in methanol (53 mL) at 0 C. was added lithium hydroxide (840 mg, 34.4 mmol) in water (35 mL). The mixture was stirred at 0 C. for 2 hours and then acidified to pH-5 with aqueous 1 M hydrochloric acid. The resulting solution was extracted with ethyl acetate (2250 mL) and washed with brine (3100 mL). The organic layer was dried over anhydrous sodium sulfate and concentrated to give (S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoic acid as a white solid that was used in the next step without further purification. ESI-MS m/z=581.4 [M+NH.sub.4].sup.+.

[0437] Step D

[0438] To a solution of the trifluoroacetic acid salt of methyl (S)-hexahydropyridazine-3-carboxylate (6.48 g, 45.0 mmol) in dichloromethane (200 mL) at 0 C. was added N-methylmorpholine (40.99 g, 405.2 mmol), (S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoic acid (24.0 g, 42.6 mmol) in dichloromethane (50 mL), HOBt (1.21 g, 9.01 mmol) and EDCl (12.9 g, 67.55 mmol). The mixture was stirred at 20 C. for 16 hours and then diluted with dichloromethane (200 mL) and washed with water (3150 mL). The organic layer was dried over anhydrous sodium sulfate and concentrated to give the crude product that was purified by silica gel chromatography (0-20% ethyl acetate in petroleum ether) to give methyl (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoyl)hexahydropyridazine-3-carboxylate (71% yield) as a yellow oil. ESI-MS m/z=690.5 [M+H].sup.+

B. Methyl (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propenoyl)hexahydropyridazine-3-carboxylate

[0439] ##STR00145##

[0440] Step A

[0441] A solution of (R)-(2-((tert-butoxycarbonyl)amino)-3-methoxy-3-oxopropyl)zinc(II) iodide (20.0 mL, mmol, 1.0 equiv), Pd(PPh.sub.3).sub.2Cl.sub.2 (1.75 g, 2.5 mmol, 0.1 equiv) and 3-bromo-5-iodopyridine (7.1 g, 25 mmol, 1.0 equiv) in DMF (10 mL) was stirred at 50 C. for 15 hours. The reaction was quenched by addition of ice water (300 mL) and extracted with ethyl acetate (3200 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give a crude product which was purified by silica gel chromatography (PE to petroleum ether/ethyl acetate=1:1) to give methyl (S)-3-(5-bromopyridin-3-yl)-2-((tert-butoxycarbonyl)amino)propanoate (3.1 g, 35% yield) as a yellow solid. ESI-MS m/z=359.1 [M+H].sup.+.

[0442] Step B

[0443] To a solution of methyl (S)-3-(5-bromopyridin-3-yl)-2-((tert-butoxycarbonyl)amino)propanoate (1.8 g, 5.0 mmol, 1.0 equiv) in MeOH (20 mL) was added LiOH (600 mg, 25.0 mmol, 5.0 equiv) in H.sub.2O (5 mL) at 0 C. The mixture was stirred at 0 C. for 5 hours. The mixture was acidified to pH5 with 1 M HCl and extracted with ethyl acetate (100 mL2). The organic layer washed with brine (100 mL3), dried over anhydrous sodium sulfate and concentrated to give a residue. The crude product (1.73 g crude) was used in the next step directly without further purification. ESI-MS m/z=345.0 [M+H].sup.+.

[0444] Step C

[0445] A solution of (S)-3-(5-bromopyridin-3-yl)-2-((tert-butoxycarbonyl)amino)propanoic acid (1.73 g, 5.0 mmol, 1.0 equiv), HATU (2.85 g, 7.5 mmol, 1.5 equiv) and DIPEA (3.23 g, 25 mmol, 5.0 equiv) in DMF (15 mL) was stirred at 0 C. for 30 minutes. Methyl (S)-hexahydropyridazine-3-carboxylate (2.23 g, 6.0 mmol, 1.2 equiv, TFA salt) in DMF (5 mL) was then added dropwise. After 2 hours, the reaction was quenched by the addition of ice water (100 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure to give a crude product, which was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH=20:1) to give methyl (S)-1-((S)-3-(5-bromopyridin-3-yl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate (1.51 g, 64% yield) as an oil. ESI-MS m/z=471.1 [M+H].sup.+.

[0446] The following intermediate was synthesized according to the procedure described to make Intermediate B using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00004 Intermediate No. Structure Analytical Data B-1 [00146] ESI-MS m/z = 520.2 [M + H].sup.+

C. Methyl (S)-1-((S)-3-(6-bromo-4((tert-butoxycarbonyl)oxy)pyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate

[0447] ##STR00147##

[0448] Step A

[0449] A solution of (R)-(2-((tert-butoxycarbonyl)amino)-3-methoxy-3-oxopropyl)zinc(II) iodide (20.0 mL, 24 mmol, 2.0 equiv). Pd(PPh.sub.3).sub.2Cl.sub.2 (1.68 g, 2.4 mmol, 0.2 equiv) and 2,6-dibromo-4-methoxypyridine (3.2 g, 12 mmol, 1.0 equiv) in DMF (10 mL) was stirred at 65 C. for 2 hours. The reaction was quenched by the addition of ice water (300 mL) and extracted with ethyl acetate (3200 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure to give a crude product that was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH=40:1) to give methyl (S)-3-(6-bromo-4-methoxypyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoate (2.4 g, 51% yield) as a yellow oil. ESI-MS m/z=389.0 [M+H].sup.+.

[0450] Step B

[0451] A solution of methyl (S)-3-(6-bromo-4-methoxypyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoate (2.4 g, 6.17 mmol, 1.0 equiv) in HBr (40% in water) (20 mL) at 130 C. was stirred for 16 hours. The mixture was concentrated to give a crude residue (2.1 g) as a yellow solid that was used in the next step without further purification. ESI-MS m/z=261.0 [M+H].sup.+.

[0452] Step C

[0453] To a stirred solution of (S)-2-amino-3-(6-bromo-4-hydroxypyridin-2-yl)propanoic acid (2.1 g, 6.17 mmol, 1.0 equiv) in THF (100 mL) was added DMAP (753 mg, 6.17 mmol, 1.0 equiv) and TEA (1.2 g, 12.34 mmol, 2.0 equiv) followed by (Boc).sub.2O (2.69 g, 12.34 mmol, 2.0 equiv). The mixture was stirred for hours and then the solution was concentrated to give a residue. The residue was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH=20:1) to give (S)-3-(6-bromo-4-((tert-butoxycarbonyl)oxy)pyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoic acid (2.15 g, 76% yield) as a yellow oil. ESI-MS m/z=460.1 [M+H].sup.+.

[0454] Step D

[0455] A solution of (S)-3-(3-bromo-5-(difluoromethyl)phenyl)-2-((tert-butoxycarbonyl)amino)propanoic acid (2.15 g, 4.66 mmol, 1.0 equiv), HATU (2.66 g, 6.99 mmol, 1.5 equiv) and DIEA (3.00 g, 23.3 mmol, 5.0 equiv) in DMF (15 mL) was stirred at 5 C. for 30 minutes. Methyl (S)-hexahydropyridazine-3-carboxylate (1.44 g, 5.6 mmol, 1.2 equiv, TFA salt) in DMF (5 mL) was added dropwise. After 2 hours, the reaction was quenched by the addition of ice water (100 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give a crude product, which was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH=40:1) to give methyl (S)-1-((S)-3-(6-bromo-4-((tert-butoxycarbonyl)oxy)pyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate (2.05 g, 75% yield) as a yellow oil. ESI-MS m/z=587.1 [M+H].sup.+.

D. 2-(6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indol-3-yl-2-methylpropanenitrile

[0456] ##STR00148##

[0457] Step A

[0458] A solution of tert-butyl 6-bromo-3-(cyanomethyl)-1H-indole-1-carboxylate (1.3 g, 3.88 mmol, 1.0 equiv) in THF (25 mL) was added LiHMDS (9.7 mL, 9.7 mmol, 2.5 equiv) at 78 C. This was followed by the addition of MeI (1.38 g, 9.72 mmol, 2.51 equiv) dropwise at 78 C. The resulting mixture was slowly warmed to room temperature and then stirred for 16 hours. The reaction was quenched by the addition of saturated aqueous NH.sub.4Cl (10 mL). The resulting mixture was diluted with water (200 mL) and then extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the crude product was purified by silica gel chromatography, eluting with petroleum ether/dichloromethane (5:1) to afford tert-butyl 6-bromo-3-(1-cyano-1-methylethyl)-1H-indole-1-carboxylate (1.2 g, 81%) as a white solid. .sup.1H NMR (300 MHz, CDCl.sub.3) 8.41 (s, 1H), 7.69 (d, J=8.5 Hz, 1H), 7.51-7.39 (m, 2H), 1.85 (s, 6H), 1.70 (s, 9H).

[0459] Step B

[0460] To a stirred solution of tert-butyl 6-bromo-3-(1-cyano-1-methylethyl)-1H-indole-1-carboxylate (1.1 g, 3.03 mmol, 1.0 equiv) in dichloromethane (20 mL) was added TFA (10 mL, 134.63 mmol, 44.5 equiv) dropwise at 0 C. The resulting mixture was stirred for 2 hours at 0 C. The mixture was then concentrated under vacuum. The resulting mixture was diluted with water (200 mL). The mixture was basified to pH 8 with saturated aqueous NaHCO.sub.3. The resulting solution was extracted with ethyl acetate (3200 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude 2-(6-bromo-1H-indol-3-yl)-2-methylpropanenitrile (750 mg, 89% yield) was used in the next step directly without further purification. ESI-MS m/z=263.1 [M+H].sup.+.

[0461] The following intermediates were synthesized according to the procedure described to make Intermediate D using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00005 Intermediate No. Structure Analytical Data D-1 [00149] ESI-MS m/z = 249.0, 251.0 [M + H].sup.+ D-2 [00150] ESI-MS m/z = 291.0 [M + H].sup.+ D-3 [00151] D-4 [00152] ESI-MS m/z = 261.0 [M + H].sup.+ D-5 [00153] ESI-MS m/z = 305.0 [M + H].sup.+ D-6 [00154] ESI-MS m/z = 289.1 [M + H].sup.+

E. 3-(6-bromo-1H-Indol-3-yl)-3-methylbutan-2-one

[0462] ##STR00155##

[0463] To a stirred solution of 2-(6-bromo-1H-indol-3-yl)-2-methylpropanenitrile (3.5 g, 0.013 mmol, 1.0 equiv) in THF (50 mL) was added MeLi (1 M, 10 equiv, 35 mL) dropwise at 0 C. The resulting mixture was stirred for 3 hours at 0 C. To the mixture was added aqueous HCl (1 L) at room temperature. The resulting mixture was stirred for 16 hours at room temperature. The reaction was quenched by the addition of aqueous NaHCO.sub.3 solution (500 mL) at room temperature. The mixture was diluted with water (300 mL) and then extracted with ethyl acetate (2300 mL). The combined organic layers were washed with water (2200 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the residue was purified by silica gel column chromatography, eluting with EA/PE (1:20-1:12) to afford 3-(6-bromo-1H-indol-3-yl)-3-methylbutan-2-one (2 g, 48% yield) as a brown solid. ESI-MS m/z=280.1 [M+H].sup.+.

F: 6-bromo-1H-indole-3-carboxamide

[0464] ##STR00156##

[0465] Step A

[0466] To a solution of 6-bromo-1H-indole-3-carboxylic acid (2.88 g, 12.0 mmol, 1.0 equiv) in dichloromethane (10 mL) and DMF (10 mL) at 0 C. was added oxalyl dichloride (4.57 g, 36.0 mmol, 3.0 equiv) dropwise. The mixture was stirred at 0 C. for 2 hours. The mixture was used in the next step directly.

[0467] Step B

[0468] To a solution of NH.sub.3.H.sub.2O (8.16 g, 120.0 mmol, 10.0 equiv, 25% NH.sub.3) in H.sub.2O (20 mL) was added 6-bromo-1H-indole-3-carbonyl chloride (reaction solvent from Step A) dropwise at 0 C. The mixture was stirred at 0 C. for 2 hours. The mixture was poured into water and extracted with ethyl acetate (200 mL). The organic layer washed with brine (350 mL), dried over anhydrous sodium sulfate and concentrated to give a residue. The residue was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH=20:1) to give 6-bromo-1H-indole-3-carboxamide (2.45 g, 85% yield) as a white solid. ESI-MS m/z=241.0 [M+H].sup.+.

[0469] The following intermediates were synthesized according to the procedure described to make Intermediate F using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00006 Intermediate No. Structure Analytical Data F-1 [00157] ESI-MS m/z = 267.0 [M + H].sup.+ F-2 [00158] ESI-MS m/z = 253.0 [M + H].sup.+

G: 6-bromo-3-(methylsulfonyl)-1H-indole

[0470] ##STR00159##

[0471] 6-Bromo-1H-indole (1.0 g, 5.13 mmol, 1.0 equiv) and tert-BuOK (1.15 g, 10.3 mmol, 2.0 equiv) in THF (15 mL) was stirred for 30 minutes at room temperature. A solution of Et.sub.3B (10.3 mL, 10.3 mmol, 2 equiv, 1 M in THF) was added dropwise over the course of 30 minutes. Methanesulfonyl chloride (1.2 g, 10.3 mmol, 2.0 equiv) was added at 15 C., and the solution was maintained at that temperature for 24 hours. The reaction was quenched by the addition of 30 mL of saturated aqueous NH.sub.4Cl. The resulting solution was extracted with ethyl acetate (330 mL) and dried over anhydrous sodium sulfate, filtered, and concentrated. The crude product was purified by Prep-HPLC (5% MeCN-95% MeCN in water w/0.05% FA) to give 6-bromo-3-(methylsulfonyl)-1H-indole (620 mg, 44% yield) of as a light green solid. ESI-MS m/z=274.0, 276.0 [M+H].sup.+.

H: 6-bromo-2-methyl-1H-indole-3-carbonitrile

[0472] ##STR00160##

[0473] To a mixture of 6-bromo-2-methyl-1H-indole-3-carbaldehyde (3.2 g, 13.4 mmol, 1.0 equiv), hydroxylamine hydrochloride (1.0 g, 14.8 mmol, 1.1 equiv), and Et.sub.3N (1.5 g, 14.8 mmol, 1.1 equiv) in DMF (30 mL) was added T.sub.3P (4.7 g, 14.8 mmol, 1.1 equiv, 50% in ethyl acetate). The mixture was stirred at 100 C. for 3 hours and then poured onto saturated aqueous NaHCO.sub.3 solution (200 mL) and extracted with ethyl acetate (350 mL). The combined organic layers were washed with H.sub.2O (50 mL) and brine (50 mL), dried over sodium sulfate, filtered, and concentrated under reduced pressure to give 6-bromo-2-methyl-1H-indole-3-carbonitrile (1.5 g, 45% yield) as a white solid. ESI-MS m/z=235.0 [M+H].sup.+.

I: 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile

[0474] ##STR00161##

[0475] Step A

[0476] To a stirred solution of 6-bromo-1H-pyrrolo[2,3-b]pyridine (6.0 g, 30 mmol, 1.0 equiv) in DMF (10 mL) at 0 C. was added phosphorus chloride oxide (90 mmol, 8.4 mL, 3.0 equiv) and the resulting mixture was stirred at room temperature. After being stirred for 1 hour, the reaction mixture was poured into cold saturated aqueous NaHCO.sub.3 solution and stirred for 30 minutes. The reaction mixture was extracted with ethyl acetate (3). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give 6-bromo-1H-pyrrolo[2,3-b]pyridine-3-carbaldehyde (6.0 g, 87% yield) as a white solid. ESI-MS m/z=225.0 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 12.91 (s, 1H), 9.94 (s, 1H), 8.51 (s, 1H), 8.34 (d, J=8.2 Hz, 1H), 7.49-7.47 (d, J=8.2 Hz, 1H).

[0477] Step B

[0478] To a mixture of 6-bromo-1H-pyrrolo[2,3-b]pyridine-3-carbaldehyde (2.24 g, 10 mmol, 1.0 equiv), hydroxylamine hydrochloride (764 mg, 11 mmol, 1.1 equiv), and triethylamine (1.11 g, 11 mmol, 1.1 equiv) in DMF (30 mL) was added T.sub.3P (3.5 g, 11 mmol, 1.1 equiv, 50% solution in ethyl acetate). The mixture was stirred at 100 C. for 3 hours. The mixture was cooled and poured into aqueous sodium bicarbonate solution (200 mL) and extracted with ethyl acetate (350 mL). The combined organic layers were washed with water (50 mL) and brine (50 mL), dried over sodium sulfate, filtered, and concentrated under reduced pressure to give the product of 6-bromo-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile (2.0 g, 91% yield) as a white solid. ESI-MS m/z=222.1 [M+H].sup.+.

J: 6-bromo-4-hydroxy-1-naphthonitrile

[0479] ##STR00162##

[0480] Step A

[0481] A solution of 7-bromonaphthalen-1-ol (100 mg, 0448 mmol, 1 equiv), MeCN (10 mL), pTsOH (77.0 mg, 0.45 mmol, 1.0 equiv), and N-iodosuccinimide (101.0 mg, 0.45 mmol 1.0 equiv) was stirred for 14 hours at 25 C. The residue was purified by silica gel chromatography (ethyl acetate/petroleum ether (5:1)) to give 7-bromo-4-iodonaphthalen-1-ol (130 mg 83% yield). ESI-MS m/z=347.0 [MH].sup..

[0482] Step B

[0483] A solution of 7-bromo-4-iodonaphthalen-1-ol (2.20 g, 6.30 mmol, 1.0 equiv), acetonitrile (40 mL), zincdicarbonitrile (1.10 g, 9.46 mmol, 1.5 equiv), and Pd(dba).sub.2 (220 mg, 0.383 mmol, 0.06 equiv) was stirred for 16 hours at 70 C. The residue was applied onto a silica gel column and eluted with ethyl acetate/hexane (5:1) to give 6-bromo-4-hydroxynaphthalene-1-carbonitrile (700 mg, 45% yield). ESI-MS m/z=246.0 [MH].sup..

K: 8-bromo-5-ethyl-1,3,4,5-tetrahydro-2H-benzo[b][1,4]diazepin-2-one

[0484] ##STR00163##

[0485] Step A

[0486] To a stirred solution of 4-bromo-2-fluoro-1-nitrobenzene (5.0 g, 22.7 mmol, 1.0 equiv) in DMF (50 mL) was added K.sub.2CO.sub.3 (6.33 g, 45.5 mmol, 2.0 equiv) and methyl 3-(ethylamino)propanoate (3.9 g, 29.7 mmol, 1.3 equiv) dropwise. The resulting mixture was stirred for 16 hours and then the mixture was diluted with 100 mL water and extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (3100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether (20:1 to 12:1) to afford methyl 3-((4-bromo-2-nitrophenyl)(ethyl)amino)propanoate (6.53 g, 85% yield) as a red oil. ES m/z=333.1 [M+H].sup.+.

[0487] Step B

[0488] To a stirred solution of 3-((4-bromo-2-nitrophenyl)(ethyl)amino)propanoate (6.52 g, 19.688 mmol, 1 equiv) in methanol (60 mL) was added acetic acid (23.7 g, 394.6 mmol, 20 equiv) and zinc (6.4 g, 99 mmol, 5.0 equiv) in portions at room temperature. The resulting mixture was stirred for 2 hours at room temperature. The precipitated solids were collected by filtration and washed with MeOH (160 mL). The resulting filtrate was stirred overnight at 80 C. The mixture was neutralized to pH 7 with aqueous saturated NaHCO.sub.3. The precipitated solids were filtered off and washed with ethyl acetate (310 mL). The resulting mixture was extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (250 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The crude residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (10:1 to 3:1) to afford 8-bromo-5-ethyl-1,3,4,5-tetrahydro-2H-benzo[b][1,4]diazepin-2-one (2.8 g, 50% yield) as a brown solid. ESI-MS m/z=269.0 [M+H].sup.+.

L: 6-bromo-3-(3,6-dihydro-2H-pyran-4-yl)-1H-indole

[0489] ##STR00164##

[0490] Step A

[0491] To a stirred solution of NaH (60% dispersion in oil, 0.4 g, 16.67 mmol, 1.25 equiv) in DMF (40 mL) was added 6-bromo-3-iodo-1H-indole (4.3 g, 13.36 mmol, 1 equiv) dropwise at 0 C. The resulting mixture was stirred for 1 hour at 0 C., and then 4-methylbenzene-1-sulfonyl chloride (5.6 g, 29.38 mmol, 2.2 equiv) was added dropwise at 0 C. The mixture was stirred for an additional 16 hours at room temperature. The reaction was poured into ice water. The aqueous layer was extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (350 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and 6-bromo-3-iodo-1-tosyl-1H-indole was used in the next step directly without further purification. .sup.1H NMR (300 MHz, CDCl.sub.3) 8.17 (d, J=1.5 Hz, 1H), 7.80 (d, J=8.4 Hz, 2H), 7.67 (s, 1H), 7.44 (dd, J=8.4, 1.6 Hz, 1H), 7.30 (d, J=8.4 Hz, 2H), 7.25 (d, J=8.4 Hz, 1H), 2.40 (s, 3H).

[0492] Step B

[0493] A solution of 6-bromo-3-iodo-1-tosyl-1H-indole (3.0 g, 6.30 mmol, 1 equiv), 2-(3,6-dihydro-2H-pyran-4-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane(4.0 g, 18.90 mmol, 3 equiv), Pd(dppf)Cl.sub.2 (0.3 g, 0.41 mmol, 0.07 equiv), and K.sub.2CO.sub.3 (4.4 g, 31.84 mmol, 5.05 equiv) in dioxane (30 mL) and H.sub.2O (6 mL) was stirred for 3 hours at 60 C. The resulting mixture was extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (330 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (10:1) to afford 6-bromo-3-(3,6-dihydro-2H-pyran-4-yl)-1-tosyl-1H-indole (2.1 g, 77% yield) as a yellow solid. .sup.1H NMR (300 MHz, CDCl.sub.3) 8.21 (d, J=1.7 Hz, 1H), 7.79 (d, J=8.4 Hz, 2H), 7.64 (d, J=8.6 Hz, 1H), 7.49 (s, 1H), 7.42-7.37 (m, 1H), 7.30 (s, 1H), 7.27 (s, 1H), 6.22 (s, 1H), 4.37 (q, J=2.6 Hz, 2H), 3.98 (t, J=5.5 Hz, 2H), 2.53 (dd, J=4.8, 2.2 Hz, 2H), 2.39 (s, 3H).

[0494] Step C

[0495] A solution of 6-bromo-3-(3,6-dihydro-2H-pyran-4-yl)-1-tosyl-1H-indole (2.1 g, 4.86 mmol, 1 equiv) and KOH (2.7 g, 48.12 mmol, 9.91 equiv) in MeOH (40 mL) and H.sub.2O (10 mL) was stirred for 3 hours at 65 C. The resulting mixture was extracted with ethyl acetate (350 mL) and the combined organic layers were washed with brine (310 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to give 6-bromo-3-(3,6-dihydro-2H-pyran-4-yl)-1H-indole that was used in the next step directly without further purification. ESI-MS m/z=278.0 [M+H].sup.+.

[0496] The following intermediate was synthesized according to the procedure described to make Intermediate L using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00007 Intermediate No. Structure Analytical Data L-1 [00165] ESI-MS m/z = 272.2 [M + H].sup.+

M: 6-bromo-2-(2-(methoxymethyl)phenyl)-1H-indole

[0497] ##STR00166##

[0498] Step A

[0499] A solution of (2-(methoxymethyl)phenyl)boronic acid (1.66 g, 10.0 mmol, 1.0 equiv), tert-butyl 6-bromo-2-iodo-1H-indole-1-carboxylate (4.2 g, 10.0 mmol, 1.0 equiv), Pd(dppf)Cl.sub.2.CH.sub.2Cl.sub.2 (408 mg, 0.5 mmol, 0.05 equiv), and K.sub.2CO.sub.3 (4.14 g, 30 mmol, 3.0 equiv) in dioxane (20 mL) and water (4 mL) was stirred at 80 C. for 5 hours. After concentration, the residue was purified by silica gel chromatography (petroleum ether) to afford tert-butyl 6-bromo-2-(2-(methoxymethyl)phenyl)-1H-indole-1-carboxylate (2.95 g, 71% yield). ESI-MS m/z: 438.0 [M+Na].sup.+.

[0500] Step B

[0501] To a stirred solution of tert-butyl 6-bromo-2-(2-(methoxymethyl)phenyl)-1H-indole-1-carboxylate (2.95 g, 7.1 mmol, 1.0 equiv) in dichloromethane (10 mL) at 0 C. was added TFA (10 mL) dropwise. The resulting mixture was stirred for 1.5 hours at 15 C., and then concentrated under vacuum. The resulting mixture was diluted with water (100 mL). The mixture was basified to pH 8 with saturated Na.sub.2CO.sub.3. The resulting mixture was extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (200 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by flash column on silica gel (petroleum ether/ethyl acetate (10:1)) to give 6-bromo-2-(2-(methoxymethyl)phenyl)-1H-indole (1.25 g, 55% yield) as a white solid. ESI-MS m/z: 316.0 [M+H].sup.+.

N: 6-bromo-3-cyclopropyl-1H-indole

[0502] ##STR00167##

[0503] Step A

[0504] A solution of 5-bromo-2-iodoaniline (5.0 g, 16.8 mmol, 1.0 equiv), Na.sub.2CO.sub.3 (4.5 g, 42.5 mmol, 2.5 equiv). Pd(PPh.sub.3).sub.2Cl.sub.2 (1.3 g, 2.0 mmol, 0.1 equiv), and (cyclopropylethynyl)trimethylsilane (3.9 g, 28.3 mmol, 1.7 equiv) was stirred at 80 C. for 15 hours. The resulting mixture was diluted with ethyl acetate and washed with brine (340 mL). The organic phase was dried over anhydrous sodium sulfate and concentrated. The residue was applied onto a silica gel column with petroleum ether/ethyl acetate (20:1) to give 3.6 g (70% yield) of 6-bromo-3-cyclopropyl-2-(trimethylsilyl)-1H-indole as a yellow oil. .sup.1H NMR (300 MHz, DMSO-d.sub.6) 10.66 (s, 1H), 7.58-7.38 (m, 2H), 7.05 (dd, J=8.5, 1.8 Hz, 1H), 1.88 (tt, J=8.4, 5.2 Hz, 1H), 0.98-0.84 (m, 2H), 0.72-0.59 (m, 2H), 0.39 (s, 9H).

[0505] Step B

[0506] To a solution of 6-bromo-3-cyclopropyl-2-(trimethylsilyl)-1H-indole (1.8 g, 5.9 mmol, 1 equiv) in THF (18 mL) was added TBAF/THF (1 M). The resulting solution was stirred for 1 hour at 70 C. After concentration, the residue was purified by silica gel chromatography (petroleum ether/ethyl acetate 1:1) to give 1.30 g (94% yield) of 6-bromo-3-cyclopropyl-1H-indole as a yellow solid. .sup.1H NMR (300 MHz, DMSO-d.sub.6) 10.89 (s, 1H), 7.71-7.31 (m, 2H), 7.21-6.96 (m, 2H), 1.90 (ddd, J=13.4, 8.5, 5.0 Hz, 1H), 0.95-0.73 (m, 2H), 0.58 (h, J=3.7 Hz, 2H).

O: 6-bromo-3-cyclobutyl-1H-indole

[0507] ##STR00168##

[0508] To a stirred solution 6-bromo-1H-indole (4.0 g, 20.40 mmol, 1 equiv) in toluene (20 mL) was added cyclobutanone (1.5 g, 21.40 mmol, 1.05 equiv) in portions. This solution was then added over the course of 30 minutes to a stirred solution of 2,2,2-trichloroacetic acid (5.0 g, 30.60 mmol, 1.50 equiv) and Et.sub.3SiH (7.1 g, 61.06 mmol, 2.99 equiv) in toluene (20 mL) at 70 C. The resulting mixture was stirred for an additional 16 hours at 70 C. at which point the mixture was concentrated under vacuum. The residue was basified to about pH 8 with 10% aqueous Na.sub.2CO.sub.3. The mixture was then extracted with ethyl acetate (3200 mL). The combined organic layers were washed with water (100 mL) and saturated NaCl (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/dichloromethane (20:1) to afford 6-bromo-3-cyclobutyl-1H-indole(1.9 g, 32% yield) as a light-yellow solid. ESI-MS m/z=250.3 [M+H].

P: 2-bromo-5-methoxy-9H-carbazole

[0509] ##STR00169##

[0510] Step A

[0511] A solution of (2-methoxyphenyl)boronic acid (1.0 g, 6.58 mmol, 1.0 equiv), 4-bromo-1-iodo-2-nitrobenzene (2.59 g, 7.90 mmol, 1.2 equiv), Pd(PPh.sub.3).sub.2Cl.sub.2 (100 mg, 0.142 mmol, 0.02 equiv), and K.sub.2CO (4.55 g, 32.9 mmol, 5.00 equiv) in dioxane (10 mL) and water (2 mL) was stirred for 15 hours at 60 C. The mixture was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (3:1) to afford 4-bromo-2-methoxy-2-nitro-1,1-biphenyl (1.3 g, 64% yield) as a yellow solid.

[0512] Step B

[0513] A solution of 4-bromo-2-methoxy-2-nitro-1,1-biphenyl (1.2 g, 3.89 mmol, 1.0 equiv). PPh.sub.3 (3.58 g, 13.63 mmol, 3.5 equiv), and 1,2-dichlorobenzene (10 mL). The reaction mixture was irradiated with microwave radiation for 12 hours at 180 C. The crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate, 100:1 to 10:1) to give 2-bromo-5-methoxy-9H-carbazole (890 mg, 83% yield) as a yellow solid. ESI-MS m/z=276.1 [M+H].sup.+.

[0514] The following intermediates were synthesized according to the procedure described to make Intermediate P using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00008 Intermediate No. Structure P-1 [00170] P-2 [00171]

Q: 6-bromo-3-(pyridin-2-ylmethyl)-1H-indole

[0515] ##STR00172##

[0516] Step A

[0517] To a stirred solution of 6-bromo-1H-indole (1.0 g, 5.10 mmol, 1.0 equiv) and pyridine-2-carbaldehyde (546 mg, 5.10 mmol, 1.0 equiv) in MeOH (10 mL) at 0 C. was added NaOH (224 mg, 5.61 mmol, 1.1 equiv) in portions. The resulting mixture was stirred for 1 hour at 0 C. and stirred for another 5 hours at room temperature. The resulting mixture was concentrated under reduced pressure and the mixture was diluted with water (30 mL). The aqueous layer was extracted with ethyl acetate (330 mL). The combined organic layers were dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude 6-bromo-1H-indol-3-yl)(pyridin-2-yl)methanol (1.5 g) was used in the next step without further purification.

[0518] Step B

[0519] A solution of crude (6-bromo-1H-indol-3-yl)(pyridin-2-yl)methanol (1.5 g, 4.948 mmol, 1.0 equiv) in dichloromethane (20 mL) was treated with TFA (6.2 g, 54.4 mmol, 11 equiv) followed by Et.sub.3SiH (633 mg, 5.44 mmol, 1.10 equiv). The resulting solution was stirred for 2 hours at room temperature. The mixture was then concentrated under vacuum and 40 mL of water was added. The resulting solution was extracted with ethyl acetate (340 mL) and dried over anhydrous sodium sulfate. After filtering and concentrating in vacuo, the residue was purified by silica gel chromatography eluting with ethyl acetate/petroleum ether (1:4) to give 6-bromo-3-[(pyridin-2-yl)methyl]-1H-indole (1.1 g, 77% yield, 2 steps). ESI-MS m/z=287.0 [M+H]+.

[0520] The following intermediates were synthesized according to the procedure described to make Intermediate Q using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00009 Intermediate No. Structure Analytical Data Q-1 [00173] ESI-MS m/z = 287.0 [M + H].sup.+ Q-2 [00174] ESI-MS m/z = 287.0 [M + H].sup.+

R: 6-bromo-3-((tetrahydrofuran-3-ylmethyl)-1H-indole

[0521] ##STR00175##

[0522] Step A

[0523] To a solution of oxolane-3-carboxylic acid (4.39 g, 37.807 mmol, 1.21 equiv) in dichloromethane (45 mL) 0 C. was added oxalyl chloride (9.5 g, 74.847 mmol, 2.39 equiv) and N,N-dimethylformamide (0.150 mL) dropwise. The resulting mixture was stirred for 2 hours at 0 C. to 25 C. under an argon atmosphere and was then concentrated under vacuum. To a stirred solution of 6-bromo-1H-indole (6.14 g, 31.3 mmol, 1.0 equiv) in dichloromethane (70 mL) at 0 C. was added tetrachlorostannane (37.3 mL) dropwise. The resulting mixture was stirred for 10 minutes at 0 C., and then tetrahydrofuran-3-carbonyl chloride and nitromethane (3.37 mL) were dropwise at 0 C. The resulting mixture was stirred for 15 hours at 0 C. to 25 C. The reaction was quenched by the addition of ice water. The precipitated solids were collected by filtration and washed with ethyl acetate (310 mL). The filtrate was extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (1100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (10:1 to 2:1) to afford 6-bromo-3-(oxolane-3-carbonyl)-1H-indole (6.13 g, 52% yield) as a brown solid. ESI-MS m/z=294.0 [M+H].sup.+.

[0524] Step B

[0525] To a stirred solution of 6-bromo-3-(oxolane-3-carbonyl)-1H-indole (6.0 g, 20.398 mmol, 1.0 equiv) was added 1 N BH.sub.3 in THF (60 mL) dropwise. The mixture was stirred for 2 hours at room temperature. The mixture was quenched with MeOH (20 mL) at 0 C. Water (100 mL) was added and the mixture was extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (20:1 to 6:1) to afford 6-bromo-3-[(oxolan-3-yl)methyl]-1H-indole (2.7 g, 44% yield) as a brown oil. ESI-MS m/z=282.0 [M+H].sup.+.

S: 3-(6-bromo-1H-indol-3-yl)propanenitrile

[0526] ##STR00176##

[0527] Step A

[0528] A solution of 6-bromo-1H-indole-3-carbaldehyde (5.0 g, 22.3 mmol, 1 equiv) in THF (50 mL) at 0 C. was treated with NaH (60%, 535 mg, 22.3 mmol, 1.0 equiv) and maintained at that temperature for 30 minutes. Diethyl (cyanomethyl)phosphonate (7.91 g, 44.632 mmol, 2.0 equiv) was added dropwise and then the reaction mixture stirred overnight at room temperature. Water was added and the organics were removed under vacuum. The resulting aqueous layer was extracted with ethyl acetate (3250 mL). The combined organic layers were washed with water (3250 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (1:1) to afford (E)-3-(6-bromo-1H-indol-3-yl)acrylonitrile (2.5 g, 45% yield) as a yellow solid. ESI-MS m/z=245.0 [MH].sup..

[0529] Step B

[0530] To a solution of (E)-3-(6-bromo-1H-indol-3-yl)acrylonitrile (2.5 g, 10.1 mmol, 1.0 equiv) in THF (15 mL) and EtOH (15 mL) was added Pd/C (10%, 500 mg, 4.7 mmol, 0.46 equiv) and the reaction was stirred under a hydrogen atmosphere for 48 hours. The mixture was filtered and the filter cake was washed with EtOH (330 mL). The filtrate was concentrated under reduced pressure and the residue was purified by reverse phase chromatography (water/MeCN 45-50% with 0.1% FA) to afford 3-(6-bromo-1H-indol-3-yl)propanenitrile (1.1 g, 44% yield) as a dark yellow oil. ESI-MS m/z=247.0 [MH].sup..

T: 6-bromo-2-(pyridin-3-ylmethyl)-1H-indole

[0531] ##STR00177##

[0532] Step A

[0533] 6-bromo-1-(phenylsulfonyl)-1H-indole (10.0 g, 29.8 mmol, 1.0 equiv) in THF (300 mL) at 78 C. was treated with LDA (2M in THF, 22.4 mL, 44.8 mmol, 1.5 equiv). The mixture was stirred at 78 C. for 0.5 hours and then pyridine-3-carbaldehyde (3.8 g, 35.8 mmol, 1.2 equiv) was added. The reaction mixture was stirred at 78 C. for 3 hours and then water (50 mL) was added. After adding additional water (1 L), the mixture was extracted with ethyl acetate (3500 mL). The combined organics were washed with brine (500 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate=1:2) to provide (6-bromo-1-(phenylsulfonyl)-1H-indol-2-yl)(pyridin-3-yl)methanol (10.8 g, 81% yield) as a yellow solid. ESI-MS m/z: 443.0 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 8.58 (d, J=1.9 Hz, 1H), 8.50 (dd, J=4.8, 1.6 Hz, 1H), 8.15-8.08 (m, 1H), 7.90-7.80 (m, 2H), 7.73-7.65 (m, 2H), 7.63-7.52 (m, 3H), 7.43 (dd, J=8.3, 1.7 Hz, 1H), 7.36 (dd, J=7.8, 4.8 Hz, 1H), 6.75 (s, 1H), 6.42 (q, J=5.5 Hz, 2H).

[0534] Step B

[0535] To a solution of (6-bromo-1-(phenylsulfonyl)-1H-indol-2-yl)(pyridin-3-yl)methanol (10.2 g, 23.0 mmol, 1.0 equiv) in TFA (50 mL) was added Et.sub.3SiH (50 mL). After stirring for 10 hours at 80 C., the reaction solution was concentrated to dryness to give a residue. The residue was purified by silica gel chromatography (petroleum ether/ethyl acetate=3:1) to give 6-bromo-1-(phenylsulfonyl)-1H-indole (9.3 g, 95% yield) as a yellow solid. ESI-MS m/z=427.0 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 8.76 (d, J=1.8 Hz, 1H), 8.71 (dd, J=5.3, 1.3 Hz, 1H), 8.17-8.13 (m, 1H), 8.13-8.09 (m, 1H), 7.92-7.85 (m, 2H), 7.73 (ddd, J=10.3, 5.0, 3.1 Hz, 2H), 7.65-7.56 (m, 2H), 7.49 (d, J=8.3 Hz, 1H), 7.43 (dd, J=8.3, 1.7 Hz, 1H), 6.52 (d, J=0.6 Hz, 1H), 4.55 (s, 2H).

[0536] Step C

[0537] To a solution of 6-bromo-1-(phenylsulfonyl)-1H-indole (9.0 g, 21.1 mmol, 1.0 equiv) in MeOH (300 mL) and water (90 mL) was added KOH (23.6 g, 42.2 mmol, 2.0 equiv). After stirring for 16 hours at 90 C., the reaction solution was concentrated to dryness to give a residue that was purified by silica gel chromatography (petroleum ether/ethyl acetate=1:1) to give 6-bromo-2-(pyridin-3-ylmethyl)-1H-indole (4.98 g, 82% yield) as a yellow solid. ESI-MS m/z=287.0 [M+H].sup.+.

U: 2-(6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indazol-3-yl)-2-methylpropanenitrile

[0538] ##STR00178##

[0539] To a stirred solution of 6-bromo-1H-indazole-3-carbaldehyde (2.24 g, 9.95 mmol, 1.0 equiv) in formamide (50 mL) and MeOH (50 mL) was added NaBH.sub.4 (1883 mg, 49.77 mmol, 5.0 equiv) portionwise. The resulting mixture was stirred for 2 hours at room temperature and then KCN (3.241 g, 49.7 mmol, 5.00 equiv) was added in portions. The resulting mixture was stirred for 16 hours at 60 C., and was then concentrated under reduced pressure. The residue was diluted with water (100 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (3100 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate to afford 2-(6-bromo-1H-indazol-3-yl)acetonitrile (900 mg, 38% yield) as a yellow solid. .sup.1H NMR (400 MHz, DMSO-d.sub.6) =13.25 (s, 1H), 7.91-7.68 (m, 2H), 7.32 (dd, J=8.7, 1.5 Hz, 1H), 4.40 (s, 2H).

[0540] Step B

[0541] To a stirred solution/mixture of 2-(6-bromo-1H-indazol-3-yl)acetonitrile (1.1 g, 4.660 mmol, 1.0 equiv), TEA (0.71 g, 6.989 mmol, 1.5 equiv) and DMAP (57 mg, 0.466 mmol, 0.1 equiv) in dichloromethane (20 mL) was added Boc.sub.2O (1.12 g, 5.13 mmol, 1.10 equiv) in portions at 0 C. The resulting mixture was stirred for 3 hours at room temperature and then extracted with dichloromethane (330 mL). The combined organic layers were washed with brine (320 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was purified by Prep-TLC (petroleum ether/ethyl acetate (5:1)) to afford tert-butyl 6-bromo-3-(cyanomethyl)1H-indazole-1-carboxylate (1.3 g, 83% yield) as a yellow solid. .sup.1H NMR (300 MHz, CDCl.sub.3) =8.43 (s, 1H), 7.75 (d, J=8.5 Hz, 1H), 7.54 (dd, J=8.6, 1.6 Hz, 1H), 4.11 (s, 2H), 1.75 (s, 9H).

[0542] Step C

[0543] To a stirred solution of tert-butyl 6-bromo-3-(cyanomethyl)-1H-indazole-1-carboxylate (2.4 g, 7.139 mmol, 1 equiv) in THF (50 mL) was added LiHMDS (21 mL) dropwise at 78 C. under an argon atmosphere. The resulting mixture was stirred for 1 hour at 78 C., and then MeI (3.04 g, 21.418 mmol, 3.00 equiv) was added dropwise over 30 minutes at 78 C. The resulting mixture was stirred for additional 16 hours at room temperature. The reaction was quenched with saturated aqueous NH.sub.4Cl at 0 C. The aqueous layer was extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (3100 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was purified by reverse phase chromatography (MeCN in water (0.1% FA), 0% to 100%) to afford tert-butyl 6-bromo-3-(2-cyanopropan-2-yl)-1H-indazole-1-carboxylate (800 mg, crude) as a yellow solid.

[0544] Step D

[0545] To a stirred solution of tert-butyl 6-bromo-3-(2-cyanopropan-2-yl)-1H-indazole-1-carboxylate (800 mg, 2.2 mmol, 1 equiv) in dichloromethane (12 mL) was added TFA (6 mL) in portions at 0 C. The resulting mixture was stirred for 2 hours at 0 C., and then concentrated under vacuum. The residue was purified by reverse phase chromatography (MeCN in water (0.1% FA), 0% to 69% gradient) to afford 2-(6-bromo-1H-indazol-3-yl)-2-methylpropanenitrile (500 mg, 86% yield) as a white solid. ESI-MS m/z=264.4 [M+H].sup.+

V: (2-(bromomethyl)butoxy)tert-butyl)diphenylsilane

[0546] ##STR00179##

[0547] A solution of 2-(((tert-butyldiphenylsilyl)oxy)methyl)butan-1-ol (2.7 g, 7.9 mmol, 1 equiv) in dichloromethane (30 mL) was treated with PPh.sub.3 (3.1 g, 12 mmol, 1.5 equiv). After cooling to 0 C., CBr.sub.4 (3.9 g, 12 mmol, 1.5 equiv) was added. The resulting solution was stirred for 1 hour at room temperature and then concentrated. The residue was purified by silica gel chromatography with petroleum ether to give (2-(bromomethyl)butoxy)(tert-butyl)diphenylsilane (2.7 g, 85% yield) as a light yellow oil. .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 7.68-7.58 (m, 4H), 7.54-7.38 (m, 6H), 3.66 (dqd, J=16.5, 10.0, 5.4 Hz, 4H), 1.78 (hept, J=6.0 Hz, 1H), 1.36 (dq, J=14.1, 7.2 Hz, 2H), 1.01 (s, 9H), 0.84 (td, J=7.6, 7.1, 1.8 Hz, 5H).

W: 3-((tert-butyldiphenylsilyl)oxy)-2-fluoropropyl Methanesulfonate

[0548] ##STR00180##

[0549] Step A

[0550] A solution of methyl 2-fluoro-3-hydroxypropanoate (5.0 g, 41 mmol, 1.0 equiv) in dichloromethane (100 mL) was treated with imidazole (5.576 g, 82 mmol, 2.0 equiv) and TBDPS-Cl (12.33 g, 45 mmol, 1.1 equiv) at 0 C. The solution was stirred for 2 hours at room temperature and then ice water (100 mL) was added. The solution was extracted with dichloromethane (2100 mL) and the organic layers were combined and washed with brine (2100 mL). The mixture was dried over anhydrous sodium sulfate. Purification by silica gel chromatography eluting with ethyl acetate/petroleum ether (1:20 to 1:5) gave methyl 3-(tert-butyldiphenylsilyloxy)-2-fluoropropanoate (16 g) as a white solid.

[0551] Step B

[0552] A solution of methyl 3-(tert-butyldiphenylsilyloxy)-2-fluoropropanoate (8 g, 22.2 mmol, 1.0 equiv) in THF (100 mL) at 0 C. was treated with LiBH.sub.4 (1.95 g, 88.8 mmol, 4.0 equiv). The solution was stirred for 15 hours at room temperature and then quenched with ice water (100 mL). After extraction with ethyl acetate (3100 mL), the organic layers were combined. The solution was dried over anhydrous sodium sulfate. The residue was purified by silica gel chromatography eluting with ethyl acetate/petroleum ether (1:10-1:3) to give 3-((tert-butyldiphenylsilyl)oxy)-2-fluoropropan-1-ol (7.0 g, 95% yield) as a colorless oil. .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 7.74-7.59 (m, 4H), 7.54-7.35 (m, 6H), 4.94 (t, J=5.6 Hz, 1H), 4.74-4.59 (m, 1H), 4.56-4.43 (m, 2H), 3.62 (m, 5.3 Hz, 2H), 1.01 (s, 9H).

[0553] Step C

[0554] A solution of 3-((tert-butyldiphenylsilyl)oxy)-2-fluoropropan-1-ol (2 g, 6.024 mmol, 1 equiv) in dichloromethane (20 mL) at 0 C. was treated with Et.sub.3N (1.22 g, 12.048 mmol, 2.0 equiv), DMAP (73 mg, 0.602 mmol, 0.05 equiv), and methanesulfonyl chloride (0.89 g, 7.831 mmol, 1.3 equiv). The solution was stirred for 3 hours at room temperature and then the crude product was purified by silica gel chromatography eluting with ethyl acetate/petroleum ether (1:10 to 1:3) to give 3-((tert-butyidiphenylsilyl)oxy)-2-fluoropropyl methanesulfonate (2.6 g, crude) as a yellow oil. ESI-MS m/z=433.2 [M+Na].sup.+.

X: 3-bromo-2-(cyclopropylmethyl)propoxy)(tert-butyl)diphenylsilane

[0555] ##STR00181##

[0556] Step A

[0557] To a stirred solution of 1,3-diethyl 2-(cyclopropylmethyl)propanedioate (2.2 g, 10.268 mmol, 1 equiv) in THF (40 mL) was added LiBH.sub.4 (1.36 g, 62.43 mmol, 6.08 equiv) portionwise at 0 C. The resulting mixture was stirred for 14 hours at 50 C., and then diluted with water (200 mL). After extraction with ethyl acetate (3100 mL), the combined organic layers were washed with brine (20 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product 2-(cyclopropylmethyl)propane-1,3-diol (1.3 g, crude) was used in the next step directly without further purification. ESI-MS m/z=131.2 [M+H].sup.+.

[0558] Step B

[0559] To a stirred solution of 2-(cyclopropylmethyl)propane-1,3-diol (1.3 g, 9.99 mmol, 1 equiv) in THF (30 mL) was added NaH (480 mg, 12 mmol, 1.2 equiv, 60% dispersion in mineral oil) portionwise at 0 C. The mixture was stirred for 1 hour at 0 C., and then TBDPSCl (2.87 g, 10.442 mmol, 1.05 equiv) was added dropwise over 15 minutes. The resulting mixture was stirred for an additional 1 hour at 0 C., and then concentrated under reduced pressure. The residue was diluted with water (200 mL) and then extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (50 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/EA (5:1) to afford 3-[(tert-butyldiphenylsilyl)oxy]-2-(cyclopropylmethyl)propan-1-ol (3.4 g, 65% yield) as a light-yellow oil. ESI-MS m/z=369.2 [M+H].sup.+.

[0560] Step C

[0561] To a stirred solution of 3-[(tert-butyldiphenylsilyl)oxy]-2-(cyclopropylmethyl)propan-1-ol (3.4 g, 9.22 mmol, 1 equiv) in dichloromethane (40 mL) was added PPh.sub.3 (9.7 g, 37.0 mmol, 4.0 equiv) and NBS (2.5 g, 14.1 mmol, 1.5 equiv) portionwise at 0 C. The resulting mixture was stirred for 14 hours at room temperature and then concentrated under reduced pressure. The resulting mixture was filtered and the filter cake was washed with petroleum ether (3100 mL). The filtrate was concentrated under reduced pressure and the residue was purified by silica gel chromatography, eluting with 100% petroleum ether to afford [3-bromo-2-(cyclopropylmethyl)propoxy](tert-butyl)diphenylsilane (2.3 g, 55% yield) as a colorless oil. ESI-MS m/z=431.1 [M+H].sup.+.

Y: (3-bromo-2-(cyclopropylmethyl)propoxy)(tert-butyl)diphenylsilane

[0562] ##STR00182##

[0563] To a stirred solution of oxalyl chloride (580 mg, 4.570 mmol, 1.5 equiv) in dichloromethane (10.0 mL) was added DMSO (714 mg, 9.1 mmol, 3.0 equiv) dropwise at 78 C. The resulting mixture was stirred for 30 minutes at 78 C. under a nitrogen atmosphere. To the above mixture was added (2S)-3-[(tert-butyldiphenylsilyl)oxy]-2-methylpropan-1-ol (1.00 g, 3.044 mmol, 1.0 equiv) dropwise over 10 min at 78 C. The resulting mixture was stirred for an additional 30 minutes at 78 C. To the above mixture was added TEA (1.23 g, 12.155 mmol, 3.99 equiv) dropwise over 10 min at 78 C. The resulting mixture was stirred for an additional 30 minutes at 78 C., and then warmed to room temperature. The mixture was diluted with water (100 mL). The resulting mixture was extracted with dichloromethane (350 mL). The combined organic layers were washed with brine (50 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (5:1) to afford (2R)-3-[(tert-butyldiphenylsilyl)oxy]-2-methylpropanal (930 mg, 84% yield) as a colorless oil. .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 9.69 (d, J=1.3 Hz, 1H), 7.64-7.57 (m, 4H), 7.49-7.41 (m, 6H), 4.02-3.78 (m, 2H), 2.63 (qddd, J=7.0, 5.8, 4.6, 1.4 Hz, 1H), 1.04 (d, J=7.0 Hz, 3H), 0.98 (s, 9H).

Z: 6-bromo-1-((1-(hydroxymethyl)cyclopropyl)methy)-1H-indole-3-carbonitrile

[0564] ##STR00183##

[0565] Step A

[0566] A solution of 6-bromo-1H-indole-3-carboxamide (1.2 g, 5.0 mmol, 1.0 equiv), K.sub.2CO.sub.3 (1.38 g, 10.0 mmol, 2.0 equiv), KI (0.83 g, 5.0 mmol, 1.0 equiv), and ((1-(bromomethyl)cyclopropyl)methoxy)(tert-butyl)diphenylsilane (2.2 g, 5.5 mmol, 1.1 equiv) in DMSO (15 mL) was stirred at 150 C. overnight. The reaction solvent was cooled to 15 C., and ice water (100 mL) was added. The resulting solution was extracted with ethyl acetate (3100 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give a crude product that was purified by silica gel chromatography (100% dichloromethane to dichloromethane/MeOH=20:1) to give 6-bromo-1-((1-(((tert-butyldiphenylsilyl)oxy)methyl)cyclopropyl)methyl)-1H-indole-3-carbonitrile (2.18 g, 80% yield) as a clear oil.

[0567] Step B

[0568] To a solution of 6-bromo-1-((1-(((tert-butyldiphenylsilyl)oxy)methyl)cyclopropyl)methyl)-1H-indole-3-carbonitrile (2.18 g, 4.0 mmol, 1.0 equiv) in THF (20 mL) was added TBAF (8.0 mL, 1 M in THF) dropwise at 0 C. The mixture was stirred at 0 C. for 2 hours. The mixture was poured into water and extracted with ethyl acetate (200 mL). The organic phase was washed with brine (50 mL3), dried over anhydrous sodium sulfate, and concentrated to give a crude residue that was purified by silica gel chromatography (petroleum ether to petroleum ether/ethyl acetate=3:1) to give 6-bromo-1-((1-(hydroxymethyl)cyclopropyl)methyl)-1H-indole-3-carbonitrile (1.05 g, 86% yield) as a white solid. ESI-MS m/z=307.0 [M+H].sup.+.

[0569] The following compounds were synthesized according to the procedure described to make Intermediate Z using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00010 Intermediate No. Structure Analytical Data Z-1 [00184] ESI-MS m/z = 349.1 [M + H].sup.+; .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 7.89 (d, J = 1.7 Hz, 1H), 7.68 (d, J = 8.5 Hz, 1H), 7.32 (s, 1H), 7.23 (dd, J = 8.5, 1.7 Hz, 1H), 4.94 (t, J = 4.9 Hz, 1H), 4.01 (s, 2H), 3.11 (d, J = 4.9 Hz, 2H), 1.76 (s, 6H), 0.84 (s, J = 6H). Z-2 [00185] ESI-MS m/z = 335.3, 337.3 [M + H].sup.+ Z-3 [00186] .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 8.31 (s, 1H), 7.98 (d, J = 1.7 Hz, 1H), 7.61 (d, J = 8.4 Hz, 1H), 7.40 (dd. J = 8.5, 1.7 Hz, 1H), 4.69 (t, J = 4.9 Hz, 1H), 4.36-4.09 (m, 2H), 3.28 (d, J = 9.9 Hz, 2H), 1.86 (t, J = 6.3 Hz, 1H), 1.39-1.13 (m, 2H), 0.85 (t, J = 7.4 Hz, 3H). Z-4 [00187] ES-MS m/z = 339.1 [M + H].sup.+ Z-5 [00188] ESI-MS m/z = 352.0 [M + H].sup.+ Z-6 [00189] Z-7 [00190] ESI-MS m/z = 375.0 [M + H].sup.+ Z-8 [00191] ESI-MS m/z = 336.1 [M + H].sup.+ Z-9 [00192] ESI-MS m/z = 348.1 [M + H].sup.+ Z-10 [00193] ESI-MS m/z = 357.1 [M + H].sup.+ Z-11 [00194] Z-12 [00195] ESI-MS m/z = 359.0 [M + H].sup.+ Z-13 [00196] ESI-MS m/z = 359.1 [M + H].sup.+ Z-14 [00197] ESI-MS m/z = 310.0 [M + H].sup.+ Z-15 [00198] (ESI m/z): 352.2 [M + H].sup.+; .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 7.67 (d, J = 1.8 Hz, 1H), 7.50 (d, J = 8.4 Hz, 1H), 7.19 (s, 1H), 7.12 (dd, J = 8.4, 1.7 Hz, 1H), 4.65 (t, J = 5.2 Hz, 1H), 4.14 (dd, J = 14.2, 6.4 Hz, 1H), 3.92 (dd, J = 14.1, 7.5 Hz, 1H), 3.71 (ddt, J = 23.4, 15.2, 7.8 Hz, 3H), 3.37 (d, J = 8.2 Hz, 2H), 3.28-3.18 (m, 2H), 2.71 (d, J = 7.5 Hz, 2H), 2.56 (d, J = 7.6 Hz, 1H), 1.98 (ddt, J = 18.1, 12.8, 6.2 Hz, 2H), 1.56 (dq, J = 13.8, 7.1 Hz, 1H), 0.78 (d, J = 6.8 Hz, 3H). Z-16 [00199] ESI-MS m/z = 321.1 [M + H].sup.+ Z-17 [00200] Z-18 [00201] ESI-MS m/z = 377.1 [M + H].sup.+ Z-19 [00202] .sup.1H-NMR (300 MHz, CDCl.sub.3) 7.57 (dd, J = 5.1, 3.3 Hz, 2H), 7.31-7.22 (m, 1H), 7.07 (s, 1H), 4.21 (dd, J = 14.4, 6.9 Hz, 1H), 3.93 (dd, J = 14.4, 7.1 Hz, 1H), 3.52 (dd, J = 5.3, 3.5 Hz, 2H), 2.90 (dddt, J = 11.3, 9.3, 5.1, 2.3 Hz, 2H), 2.77-2.60 (m, 2H), 2.42 (dtt, J = 11.7, 9.1, 7.5 Hz, 1H), 2.30-2.09 (m, 2H), 0.98 (d, J = 6.9 Hz, 3H). Z-20 [00203] ESI-MS m/z = 347.1 [M + H].sup.+; .sup.1H-NMR (400 MHz, DMSO-d.sub.6) 7.88 (d, J = 1.5 Hz, 1H), 7.62 (d, J = 8.4 Hz, 1H), 7.37 (s, 1H), 7.25 (dd, J = 8.4, 1.7 Hz, 1H), 4.93 (t, J = 4.9 Hz, 1H), 3.97 (s, 2H), 3.09 (d, J = 5.0 Hz, 2H), 1.65 (q, J = 4.5 Hz, 2H), 1.35 (q, J = 4.8 Hz, 2H), 0.82 (s, 6H). Z-21 [00204] ESI-MS m/z = 377.0 [M + H].sup.+ Z-22 [00205] ESI-MS m/z = 359.1 [M + H].sup.+ Z-23 [00206] ESI-MS m/z = 296.1 [M + H].sup.+ Z-24 [00207] ESI-MS m/z = 339.1 [M + H].sup.+. Z-25 [00208] ESI-MS m/z = 353.1 [M + H].sup.+ Z-26 [00209] ESI-MS m/z = 341.2 [M + H].sup.+ Z-27 [00210] ESI-MS m/z = 325.1 [M + H].sup.+ Z-28 [00211] ESI-MS m/z = 424.2 [M + H].sup.+ Z-29 [00212] ESI-MS m/z = 332.0 [M + H].sup.+ Z-30 [00213] ESI-MS m/z = 346.0 [M + H].sup.+ Z-31 [00214] ESI-MS m/z = 316.0 [M + H].sup.+ Z-32 [00215] ESI-MS m/z = 353.1 [M + Na].sup.+; .sup.1H-NMR (400 MHz, CDCl.sub.3) 7.75 (d, J = 3.4 Hz, 1H), 7.49 (d, J = 8.4 Hz, 1H), 7.33 (d, J = 8.4, 1.6 Hz, 1H), 4.07 (s, 2H), 3.38 (s, 2H), 2.62 (s, 3H), 1.73 (s, 1H), 0.97 (s, 6H) Z-33 [00216] ESI-MS m/z = 279.1 [M + H].sup.+ Z-34 [00217] ESI-MS m/z = 304.9 [M + H].sup.+ Z-35 [00218] .sup.1H-NMR (300 MHz, CDCl.sub.3): = 7.66 (s, 1H), 7.54 (d, J = 8.6 Hz, 1H), 7.32 (dd, J = 8.5, 1.6 Hz, 1H), 7.16 (d, J = 0.9 Hz, 1H), 4.45 (dd, J = 14.7, 7.0 Hz, 1H), 4.11 (dd, J = 14.7, 7.5 Hz, 1H), 3.58 (dd, J = 5.3, 2.6 Hz, 2H), 2.42-2.28 (m, 1H), 1.02 (d, J = 6.9 Hz, 3H). Z-36 [00219] ESI-MS m/z = 318.1 [M + H].sup.+. Z-37 [00220] .sup.1H-NMR (300 MHz, Methanol-d.sub.4) 7.78 (d, J = 8.6 Hz, 1H), 7.69 (d, J = 1.7 Hz, 1H), 7.67-7.58 (m, 2H), 7.49-7.38 (m, 3H), 7.25 (tt, J = 8.5, 1.5 Hz, 2H), 4.31 (dd, J = 14.3, 6.5 Hz, 1H), 4.03 (dd, J = 14.3, 7.7 Hz, 1H), 3.54-3.39 (m, 2H), 2.23 (dq, J = 13.0, 6.5 Hz, 1H), 0.95 (d, J = 6.9 Hz, 3H). Z-38 [00221] .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 7.65 (d, J = 1.7 Hz, 1H), 7.54 (d, J = 8.4 Hz, 1H), 7.12 (dd, J = 8.4, 1.6 Hz, 1H), 7.06 (s, 1H), 4.65 (t, J = 5.1 Hz, 1H), 4.09 (dd, J = 14.1, 6.5 Hz, 1H), 3.87 (dd, J = 14.2, 7.5 Hz, 1H), 3.28-3.13 (m, 2H), 1.90 (td, J = 8.2, 4.0 Hz, 1H). 0.92-0.82 (m, 2H), 0.78 (d, J = 6.7 Hz, 3H), 0.61-0.52 (m, 2H). Z-39 [00222] ESI-MS m/z = 322.3 [M + H].sup.+ Z-40 [00223] ESI-MS m/z = 344.0 [M + H].sup.+ Z-41 [00224] ES-MS m/z = 359.1 [M + H].sup.+ Z-42 [00225] ESI-MS m/z = 361.2 [M + H].sup.+ Z-43 [00226] ESI-MS m/z = 293.0 [M + H].sup.+ Z-44 [00227] Z-45 [00228] ESI-MS m/z = 402.1 [M + H].sup.+ Z-46 [00229] ESI-MS m/z = 284.1, 286.1 [M + H].sup.+. Z-47 [00230] ESI-MS m/z = 308.0 [M + H].sup.+ Z-48 [00231] ESI-MS m/z = 283.1 [M + H].sup.+ Z-49 [00232] ESI-MS m/z = 341.2 [M + H].sup.+; .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 7.55 (d, J = 2.3 Hz, 1H), 7.38 (dd, J = 8.6, 2.3 Hz, 1H), 7.07 (d, J = 8.7 Hz, 1H), 4.31 (s, 1H), 3.20 (ddt, J = 31.3, 10.9, 5.7 Hz, 4H), 3.07 (s, 2H), 2.26 (d, J = 7.4 Hz, 2H), 1.55 (s, 1H), 1.02 (t, J = 7.1 Hz, 3H), 0.74 (d, J = 6.8 Hz, 3H) Z-50 [00233] ESI-MS m/z 408.8 [M + H].sup.+

AA: 4-(3-hydroxypropoxy)-2-iodobenzo[b]thiophene-7-carbonitrile

[0570] ##STR00234##

[0571] Step A

[0572] To a stirred solution of 1-benzothiophen-4-ol (2.0 g, 13.32 mmol, 1.0 equiv) in acetonitrile (20 mL) at 0 C. was added N-bromosuccinimide (2.5 g, 14.05 mmol, 1.05 equiv) portionwise. The resulting mixture was stirred for 16 hours at room temperature. The resulting residue was purified by silica gel chromatography (ethyl acetate/petroleum ether (1:4)) to afford 7-bromo-1-benzothiophen-4-ol (2.0 g, 59% yield) as a light yellow solid. .sup.1H-NMR (300 MHz, CDCl.sub.3) 7.58 (d, J=5.5 Hz, 1H), 7.46 (d, J=5.5 Hz, 1H), 7.35 (d, J=8.2 Hz, 1H), 6.68 (d, J=8.2 Hz, 1H).

[0573] Step B

[0574] A solution of 7-bromo-1-benzothiophen-4-ol (2.0 g, 8.73 mmol, 1 equiv), 3-bromopropyl acetate (1.89 g, 10.44 mmol, 1.2 equiv), and Cs.sub.2CO.sub.3 (4.29 g, 13.17 mmol, 1.51 equiv) in DMF (20 mL) was stirred for 16 hours at room temperature. The mixture was diluted with water (200 mL) and extracted with ethyl acetate (3150 mL). The combined organic layers were washed with brine and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether (1:4)) to afford 3-[(7-bromo-1-benzothiophen-4-yl)oxy]propyl acetate (2.5 g, 78% yield) as a light yellow liquid. .sup.1H-NMR (300 MHz, CDCl.sub.3)) 7.60 (d, J=5.4 Hz, 1H), 7.46-7.36 (m, 2H), 6.68 (d, J=8.3 Hz, 1H), 4.34 (t, J=6.3 Hz, 2H), 4.21 (t, J=6.1 Hz, 2H), 2.24 (h, J=6.6 Hz, 2H), 2.08 (s, 3H).

[0575] Step C

[0576] A solution of 3-[(7-bromo-1-benzothiophen-4-yl)oxy]propyl acetate (2.5 g, 7.59 mmol, 1.0 equiv), N,N-dimethylformamide (25 mL), Zn(CN).sub.2 (1.55 g, 15.07 mmol, 1.98 equiv), and Pd(PPh.sub.3).sub.4 (1.76 g, 1.52 mmol, 0.2 equiv) was stirred for 16 hours at 130 C. The resulting mixture was diluted with water (250 mL) and extracted with ethyl acetate (3150 mL). The combined organic layers were washed with brine, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether 1:4) to afford 3-[(7-cyano-1-benzothiophen-4-yl)oxy]propyl acetate(1.6 g, 69% yield) as a yellow solid. ESI-MS m/z=276.1 [M+H].sup.+.

[0577] Step D

[0578] To a stirred solution of 3-[(7-cyano-1-benzothiophen-4-yl)oxy]propyl acetate (1.6 g, 5.81 mmol, 1 equiv) in THF (16 mL) was added LiOH (698 mg, 29.2 mmol, 5.0 equiv) portionwise at 0 C. The resulting mixture was warmed to room temperature and stirred for 16 hours at room temperature. The mixture was diluted with water (100 mL) and extracted with ethyl acetate (370 mL). The combined organic layers were washed with brine, dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to afford 4-(3-hydroxypropoxy)-1-benzothiophene-7-carbonitrile (1.5 grams, 99% yield) as a purple solid. .sup.1H-NMR (300 MHz, Methanol-d.sub.4) 7.76 (d, J=8.2 Hz, 1H), 7.67 (d, J=5.5 Hz, 1H), 7.58 (d, J=5.5 Hz, 1H), 7.03 (d, J=8.3 Hz, 1H), 4.36 (t, J=6.2 Hz, 2H), 3.83 (t, J=6.2 Hz, 2H), 2.13 (p, J=6.2 Hz, 2H).

[0579] Step E

[0580] To a stirred solution of 4-(3-hydroxypropoxy)-1-benzothiophene-7-carbonitrile (1.5 g, 6.43 mmol, 1 equiv) in tetrahydrofuran (15 mL) at 0 C. was added NaH (60% dispersion in mineral oil, 387 mg, 9.68 mmol, 1.50 equiv) portionwise. The resulting mixture was stirred for 30 minutes at 0 C. at which point TBSCl (1.16 grams, 7.70 mmol, 1.20 equiv) was added portionwise. The resulting mixture was stirred for an additional 4 hours at room temperature. The mixture was then neutralized to pH 7.0 with saturated aqueous ammonium chloride. The resulting mixture was extracted with ethyl acetate and the combined organic layers were washed with brine and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the residue was purified by silica gel chromatography (ethyl acetate/petroleum ether (1:4)) to afford 4-[3-[(tert-butyldimethylsilyl)oxy]propoxy]-1-benzothiophene-7-carbonitrile (2.1 g, 75% yield) as a light yellow liquid. .sup.1H-NMR (300 MHz, CDCl.sub.3) 7.68 (d, J=8.2 Hz, 1H), 7.56 (d, J=5.4 Hz, 1H), 7.48 (d, J=5.5 Hz, 1H), 6.85 (d, J=8.3 Hz, 1H), 4.31 (t, J=6.2 Hz, 2H), 3.88 (t, J=5.9 Hz, 2H), 2.12 (p, J=6.1 Hz, 2H), 0.91 (s, 9H), 0.06 (s, 6H).

[0581] Step F

[0582] To a stirred solution of 4-[3-[(tert-butyldimethylsilyl)oxy]propoxy]-1-benzothiophene-7-carbonitrile (800 mg, 2.30 mmol, 1 equiv) in THF was added lithium diisopropylamine in THF (1 M, 3.4 mL, 3.4 mmol, 1.50 equiv) dropwise at 60 C. The resulting mixture was stirred for 1 hour at 30 C., and then N-iodosuccinimide (778 mg, 3.46 mmol, 1.50 equiv) was added in portions at 60 C. The resulting mixture was stirred for an additional 16 hours at room temperature. The reaction was quenched by the addition of saturated aqueous ammonium chloride (200 mL). The resulting mixture was extracted with ethyl acetate (3100 mL). The combined organic layers were washed with water (1100 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether (1:1)) to afford 4-[3-[(tert-butyldimethylsilyl)oxy]propoxy]-2-iodo-1-benzothiophene-7-carbonitrile (800 mg, 62% yield) as a yellow solid. .sup.1H-NMR (300 MHz, CDCl.sub.3) 7.75 (d, J=1.6 Hz, 1H), 7.60 (d, J=8.3 Hz, 1H), 6.80 (d, J=8.4 Hz, 1H), 4.29 (t, J=6.3 Hz, 2H), 3.86 (t, J=5.9 Hz, 2H), 2.11 (q, J=7.4, 6.8 Hz, 2H), 0.91 (d, J=1.8 Hz, 9H), 0.07 (s. J=1.7 Hz, 6H).

[0583] Step G

[0584] To a stirred solution of 4-[3-[(tert-butyldimethylsilyl)oxy]propoxy]-2-iodo-1-benzothiophene-7-carbonitrile (800 mg, 1.69 mmol, 1 equiv) in THF (10 mL) at 0 C. was added TBAF (1.0 M in THF, 2 mL) dropwise. After completion of the reaction as monitored by LC-MS, the mixture was concentrated under reduced pressure. The residue was purified by silica gel chromatography (ethyl acetate/petroleum ether 1:1) to afford 4-(3-hydroxypropoxy)-2-iodo-1-benzothiophene-7-carbonitrile (500 mg, 74.14% yield) as a yellow solid. .sup.1H-NMR (300 MHz, CDCl.sub.3) 7.73 (s, 1H), 7.56 (t, J=8.1 Hz, 1H), 6.79 (d, J=8.4 Hz, 1H), 4.33 (t, J=6.3 Hz, 2H), 4.02-3.84 (m, 2H), 2.17 (p, J=6.2 Hz, 2H).

AB: 3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)-2,2-dimethylpropan-1-ol

[0585] ##STR00235##

[0586] Step A

[0587] To a stirred solution of 4-bromo-2-fluoro-1-nitrobenzene (1.0 g, 45.4 mmol, 1.0 equiv) at 0 C., in DMSO (5.0 mL) was added K.sub.2CO.sub.3 (1.25 g, 90.8 mmol, 2.0 equiv) followed by 3-amino-2,2-dimethylpropan-1-ol (0.70 g, 68.1 mmol, 1.5 equiv). The mixture was stirred for 2 hours at 25 C. The solution was diluted with ethyl acetate (50 mL) and water (50 mL). The layers was separated and the organic layer was washed with water (230 mL), brine (20 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure to give 3-((5-bromo-2-nitrophenyl)amino)-2,2-dimethylpropan-1-ol (1.3 g, 95% yield) as a yellow solid. ESI-MS m/z=303.0 [M+H].sup.+. .sup.1H NMR (400 MHz. DMSO-d.sub.6) d 8.59 (t, J=4.7 Hz, 1H), 7.98 (d, J=9.1 Hz, 1H), 7.31 (s, 1H), 6.80 (d, J=9.1 Hz, 1H). 5.02 (t, J=4.9 Hz, 1H), 3.28 (d, J=4.9 Hz, 2H), 3.21 (d, J=5.2 Hz, 2H), 0.93 (s, 6H).

[0588] Step B

[0589] To a stirred suspension of 3-((5-bromo-2-nitrophenyl)amino)-2,2-dimethylpropan-1-ol (1.3 g, 4.3 mmol, 1.0 equiv) and iron powder (1.2 g, 21.5 mmol, 5.0 equiv) in ethanol (10 mL) and water (10 mL) was added NH.sub.4Cl (690 mg, 12.9 mmol, 3.0 equiv). After stirring at 70 C. for 1 hour, the mixture was filtered. The filtrate was concentrated under reduced pressure to provide crude 3-((2-amino-5-bromophenyl)amino)-2,2-dimethylpropan-1-ol (1.2 grams) as a brown oil. ESI-MS m/z=273.1 [M+H].sup.+.

[0590] Step C

[0591] A solution of 3-((2-amino-5-bromophenyl)amino)-2,2-dimethylpropan-1-ol (6.0 g, 22.0 mmol, 1.2 eq), 1,1,1-trimethoxyethane (20 mL) and concentrated hydrochloric acid (3.0 mL) was stirred at 25 C. for 16 hours. After concentration, the residue was purified by silica gel chromatography (petroleum/ethyl acetate=3/1 to 1/1) to afford the 3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)-2,2-dimethylpropan-1-ol (4.1 g, 63% yield) as an off-white solid. ESI-MS m/z=297.1 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.89 (d, J=1.7 Hz, 1H), 7.44 (d, J=8.5 Hz, 1H), 7.25 (dd, J=8.5, 1.8 Hz, 1H), 5.01 (t, J=5.0 Hz, 1H), 4.04 (s, 2H), 3.15 (d, J=5.0 Hz, 2H), 2.54 (s, 3H), 0.86 (s, 6H).

[0592] The following intermediates were synthesized according to the procedure described to make Intermediate AB using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00011 Intermediate No. Structure Analytical Data AB-1 [00236] ESI-MS m/z = 304.0 [M + H].sup.+ AB-2 [00237] ESI-MS m/z = 305.0 [M + H].sup.+ AB-3 [00238] ESI-MS m/z = 295.1 [M + H].sup.+. AB-4 [00239] ESI-MS m/z = 315.0 [M + H].sup.+ AB-5 [00240] ESI-MS m/z = 283.0 [M + H].sup.+ AB-6 [00241] ESI-MS m/z = 285.1 [M + H].sup.+

AC: 3-(6-bromo-2-(methoxymethyl)-1H-benzo[d]imidazol-1-yl)-2,2-dimethylpropan-1-ol

[0593] ##STR00242##

[0594] Step A

[0595] To a stirred solution of 3-((2-amino-5-bromophenyl)amino)-2,2-dimethylpropan-1-ol (10.0 g, 37 mmol, 1.0 equiv), imidazole (12.6 g, 185 mmol, 5.0 equiv) and DMAP (22.0 g, 183 mmol, 5.0 equiv) in dichloromethane (250 mL) was added TIPSCl (35.0 g, 183 mmol, 5.0 equiv). The mixture was stirred for 48 hours and then the solution was poured into water (500 mL) and extracted with ethyl acetate (300 mL3). The combined organic layers were washed with water (300 mL2) and brine (300 mL), dried over sodium sulfate and purified by silica gel chromatography (cyclohexane/ethyl acetate: 1:3-2:1) to give 5-bromo-N.sup.1-(2,2-dimethyl-3-((triisopropylsilyl)oxy)propyl)benzene-1,2-diamine (2.5 g, 37% yield) as a black oil. ESI-MS m/z=429.2 [M+H].sup.+.

[0596] Step B

[0597] To a stirred solution of 5-bromo-N.sub.1-(2,2-dimethyl-3-((triisopropylsilyl)oxy)propyl)benzene-1,2-diamine and (10.0 g, 9.3 mmol, 1.0 equiv) and 2-methoxyacetic acid (922 mg, 10.2 mmol, 1.1 equiv) in DMF (50 mL) was added DIPEA (6.0 g, 46.5 mmol, 5.0 equiv) followed by HATU (5.3 g, 13.9 mmol, 1.5 equiv). The resulting solution was stirred for 1 hour and then the solution was diluted with ethyl acetate (20 mL) and water (20 mL). The layers was separated and the organic layer was washed with water (320 mL), brine (10 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure to give the N-(4-bromo-2-((2,2-dimethyl-3-((triisopropylsilyl)oxy)propyl)amino)phenyl)-2-methoxyacetamide (12.0 g) as an oil. The crude product was used in the next step without further purification. ESI-MS m/z=501.3[M+H].sup.+.

[0598] Step C

[0599] A solution of N-(4-bromo-2-((2,2-dimethyl-3-((triisopropylsilyl)oxy)propylamino)-phenyl)-2-methoxyacetamide (10.5 g, 20.9 mmol, 1.0 equiv) in AcOH (110 mL) was stirred for 16 hours at 75 C. After concentration, the crude product was purified by silica gel chromatography (petroleum ether:ethyl acetate (3:1)) to afford 6-bromo-1-(2,2-dimethyl-3-((triisopropylsilyl)oxy)propyl)-2-(methoxymethyl)-1H-benzo[d]imidazole (3.4 g, 57% yield) as a brown oil. ESI-MS m/z=483.2 [M+H].sup.+.

[0600] Step D

[0601] A solution of 6-bromo-1-(2,2-dimethyl-3-((triisopropylsilyl)oxy)propyl)-2-(methoxymethyl)-1H-benzo[d]imidazole (3.4 g, 7.0 mmol, 1.0 eq) in HCl/MeOH (10 M, 18 mL) was stirred for 1 hour. After concentration, the crude product was washed with ether (20 mL) and then the product was filtered to give 3-(6-bromo-2-(methoxymethyl)-1H-benzo[d]imidazol-1-yl)-2,2-dimethylpropan-1-ol as a brown solid (1.5 g, 65% yield). ESI-MS m/z=327.1[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 7.99 (d, J=1.7 Hz, 1H), 7.56 (d, J=8.5 Hz, 1H), 7.32 (dd, J=8.5, 1.8 Hz, 1H), 5.05 (t, J=5.0 Hz, 1H), 4.72 (s, 2H), 4.16 (s, 2H), 3.31 (s. 3H), 3.14 (d, J=5.0 Hz, 2H), 0.86 (s, 6H).

[0602] The following intermediate was synthesized according to the procedures described for intermediate AC using appropriate building blocks and modified reaction conditions (such as reagent ratio, temperature, coupling conditions, and reaction time) as needed.

TABLE-US-00012 Intermediate No. Structure Analytical Data AC-1 [00243] ESI-MS m/z = 339.0 [M + H]+

AD: 3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)butan-1-ol

[0603] ##STR00244##

[0604] A solution of 3-((2-amino-5-bromophenyl) amino) propan-1-ol (3.0 g, 12.3 mmol, 1.0 equiv) and 3-methoxybenzaldehyde (1.7 g, 12.3 mmol, 1.0 equiv) was stirred in DMSO (15 mL) at 40 C. for 16 hours. Water (10 mL) was added and the solution was extracted with ethyl acetate (20 mL3). The combined organic layers were concentrated under reduced pressure to give a residue that was purified by silica gel chromatography (petroleum ether/ethyl acetate (3/1 to 1/1)) to afford the product of 3-(6-bromo-2-(3-methoxyphenyl)-1H-benzo[d]imidazol-1-yl)propan-1-ol (2.5 g, 57% yield) as a colorless oil. ESI-MS m/z=361.0 [M+H].sup.+.

[0605] The following Intermediates were synthesized according to the procedures described for the synthesis of intermediate AD using appropriate building blocks and modified reaction conditions (such as reagent ratio, temperature, coupling conditions, and reaction time) as needed

TABLE-US-00013 Intermediate No. Structure Analytical Data AD-1 [00245] ESI-MS m/z = 356.1 [M + H]+ AD-2 [00246] ESI-MS m/z = 332.0 [M + H]+ AD-3 [00247] ESI-MS m/z = 356.0 [M + H]+. AD-4 [00248] ESI-MS m/z = 356.0 [M + H]+. AD-5 [00249] ESI-MS m/z = 361.0 [M + H]+.

AE: (S)-2-(4-(2-chloroacetyl)-2-oxopiperazin-1-yl)-3-methylbutanoic Acid

[0606] ##STR00250##

[0607] Step A

[0608] A solution of 5-bromo-2-nitroaniline (1 g, 4.61 mmol, 1 equiv) in DMF (12 mL) at 0 C. was treated with NaH (60%, 222 mg, 9.25 mmol, 2.01 equiv). After 30 minutes, di-tert-butyl dicarbonate (1.2 g, 5.53 mmol, 1.2 equiv) was added. The resulting solution was stirred for 2 hours at 0 C., and then water was added. The solution was extracted with ethyl acetate (3250 mL) and the organic layers were combined and dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified by silica gel chromatography with ethyl acetate/petroleum ether (1:2) to give tert-butyl N-(5-bromo-2-nitrophenyl)carbamate (1.4 g, 96% yield) as a yellow solid. ESI-MS m/z=316.9 [M+H].sup.+.

[0609] Step B

[0610] A solution of tert-butyl N-(5-bromo-2-nitrophenyl)carbamate (600 mg, 1.89 mmol, 1 equiv), (2R)-3-bromo-2-methylpropyl acetate (443 mg, 2.27 mmol, 1.2 equiv), MeCN (10 mL), KI (31 mg, 0.19 mmol, 0.1 equiv), and Cs.sub.2CO.sub.3 (1232.8 mg, 3.78 mmol, 2.0 equiv) was stirred for 15 hours at 65 C. The solids were filtered off and the filtrate was concentrated. The residue was applied onto a silica gel column with ethyl acetate/petroleum ether (1:5) to give (2S)-3-[(5-bromo-2-nitrophenyl)[(tert-butoxy)carbonyl]amino]-2-methylpropyl acetate (580 mg, 64%) as a yellow oil.

[0611] Step C

[0612] A solution of (2S)-3-[(5-bromo-2-nitrophenyl)[(tert-butoxy)carbonyl]amino]-2-methylpropyl acetate (580 mg, 1.34 mmol, 1 equiv), dichloromethane (6 mL) and TFA (3 mL) was stirred for 1 hour at 0 C. The resulting mixture was concentrated to give (2S)-3-[(5-bromo-2-nitrophenyl)amino]-2-methylpropyl acetate (600 mg) as a red oil. ESI-MS m/z=289.1 [M+H].sup.+.

[0613] Step D

[0614] A solution of (2S)-3-[(5-bromo-2-nitrophenyl)amino]-2-methylpropyl acetate (600 mg, 1.81 mmol, 1 equiv), CH.sub.3COOH (3 mL), H.sub.2O (3 mL, 166.53 mmol, 91.91 equiv), and zinc (592.3 mg, 9.06 mmol, 5 equiv) was stirred for 1 hour at 0 C. After warming to room temperature and stirring for an additional 2 hours at 110 C., the solution was neutralized to about pH 7 with aqueous Na.sub.2CO.sub.3. The resulting solution was extracted with ethyl acetate (380 mL), washed with brine, dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was applied onto a silica gel column with ethyl acetate/petroleum ether (1:2) to give (2S)-3-(6-bromo-2-methy-1H-1,3-benzodiazol-1-yl)-2-methylpropan-1-ol (260 mg, 51% yield) as a black oil. ESI-MS m/z=283.1 [M+H].sup.+.

AF: (R)-3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)-2-methylpropyl Acetate

[0615] ##STR00251##

[0616] Step A

[0617] To a stirred solution of tert-butyl (5-bromo-2-nitrophenyl)carbamate (630 mg, 1.99 mmol, 1.0 equiv), Cs.sub.2CO.sub.3 (1.3 g, 3.99 mmol, 1.0 equiv), and KI (67.4 mg, 0.41 mmol, 0.18 equiv) in MeCN (10 mL) was added (S)-3-bromo-2-methylpropyl acetate (440 mg, 2.26 mmol, 1.2 equiv) at room temperature. The resulting mixture was stirred for 3 hours at 65 C., and then concentrated under vacuum. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (3:1) to afford (R)-3-((5-bromo-2-nitrophenyl)amino)-2-methylpropyl acetate (400 mg, 54% yield) as a light yellow oil. ESI-MS m/z=331.0 [M+H].sup.+.

[0618] Step B

[0619] To a stirred solution of (R)-3-((5-bromo-2-nitrophenyl)amino)-2-methylpropyl acetate (400 mg, 1.21 mmol, 1 equiv) in acetic acid (5 mL) and water (5 mL) was added zinc (380 mg, 5.81 mmol, 4.81 equiv) at room temperature. The resulting mixture was stirred for 2 hours at 110 C. and then diluted with water (100 mL). The mixture was neutralized to pH 7 with aqueous saturated sodium bicarbonate. The resulting solution was extracted with ethyl acetate (2100 mL). The combined organic layers were washed with brine (2100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (1:1) to afford (R)-3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)-2-methylpropyl acetate (280 mg, 64% yield) as an off-white solid. ESI-MS m/z=325.1 [M+H].sup.+.

AG: 3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)butan-1-ol

[0620] ##STR00252##

[0621] Step A

[0622] A solution of 5-bromo-1,3-difluoro-2-nitrobenzene (5.0 g, 21 mmol, 1.0 equiv), 3-aminopropan-1-ol (1.6 g, 21 mmol, 2 equiv) and K.sub.2CO.sub.3 (8.7 g, 63 mmol, 3.0 equiv) in DMF (70 mL) was stirred for 3 hours at room temperature. The resulting solution was diluted with 100 mL of H.sub.2O. The solution was extracted with ethyl acetate (3100 mL). The organics were washed with brine (3100 mL). The mixture was dried over anhydrous sodium sulfate and the residue was applied onto a silica gel column eluting with ethyl acetate/petroleum ether (3:2) to give 5.5 g (89% yield) of 3-((5-bromo-3-fluoro-2-nitrophenyl)amino)propan-1-ol as a yellow solid. ESI-MS m/z=293.0 [M+H].sup.+.

[0623] Step B

[0624] 3-[(5-bromo-3-fluoro-2-nitrophenyl)amino]propan-1-ol (2.5 g, 8.56 mmol, 1.0 equiv) and tetraethylammonium cyanide (1.6 g, 10.3 mmol, 1.2 equiv) were stirred in MeCN (30 mL) for 20 minutes at 55 C. The resulting mixture was concentrated and the residue was applied onto a silica gel column eluting with ethyl acetate/petroleum ether (1:1) to give 2.6 g of 5-bromo-3-((3-hydroxypropyl)amino)-2-nitrobenzonitrile as a yellow solid. ESI-MS m/z=300.0 [M+H].sup.+.

[0625] Step C

[0626] A solution of 5-bromo-3-[(3-hydroxypropyl)amino]-2-nitrobenzonitrile (900 mg, 3 mmol, 1.0 equiv) and zinc (960 mg 15 mmol, 5.0 equiv) in AcOH (9 mL) and H.sub.2O (9 mL) was stirred for 1 hour at room temperature. The solution was basified to pH 8 with aqueous NaHCO.sub.3. The resulting solution was extracted with ethyl acetate (320 mL), dried over anhydrous sodium sulfate and concentrated under vacuum to give 720 mg (89% yield) of 2-amino-5-bromo-3-((3-hydroxypropyl)amino)benzonitrile as brown oil and was used without further purification. ESI-MS m/z=270.0 [M+H].sup.+.

[0627] Step D

[0628] A solution of 2-amino-5-bromo-3-[(3-hydroxypropyl)amino]benzonitrile (600 mg, 2.23 mmol, 1.0 equiv) in formic acid (2 mL) and aqueous HCl (9 mL) was stirred for 2 hours at 110 C. The solution was basified to pH 8 with aqueous Na.sub.2CO.sub.3. The resulting solution was extracted with ethyl acetate (320 mL), dried over anhydrous sodium sulfate, and concentrated under vacuum. The residue was applied onto a silica gel column eluting with ethyl acetate/petroleum ether (7:3) to give 376 mg (60% yield) of 6-bromo-1-(3-hydroxypropyl)-1H-benzo[d]imidazole-4-carbonitrile as a brown solid. ESI-MS m/z=280.0 [M+H].sup.+.

AH: 3-(6-bromo-3-(3,6-dihydro-2H-pyran-4-yl)-1H-Indol-1-yl)propyl Acetate

[0629] ##STR00253##

[0630] A solution of 6-bromo-3-(3,6-dihydro-2H-pyran-4-yl)-1H-indole (1.4 g, 4.89 mmol, 1.0 equiv), 3-bromopropyl acetate (1.2 g, 6.36 mmol, 1.3 equiv), and Cs.sub.2CO.sub.3 (3.2 g, 9.82 mmol, 2.01 equiv) in DMF (20 mL) was stirred for 16 hours at room temperature. The reaction was poured into water (100 mL) and then extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (550 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by prep-TLC (petroleum ether/ethyl acetate 4:1) to afford 3-(6-bromo-3-(3,6-dihydro-2H-pyran-4-yl)-1H-indol-1-yl)propyl acetate (1.7 g, 92% yield) as a yellow oil. ESI-MS m/z=380.1 [M+H].sup.+.

[0631] The following intermediates were synthesized according to the procedure described to make Intermediate AH using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00014 Intermediate No. Structure Analytical Data AH-1 [00254] ESI-MS m/z = 335.0, 337.0 [M + H]+ AH-2 [00255] .sup.1H NMR (300 MHz, DMSO-d.sub.6) 7.93 (s, 1H), 7.70 (d, J = 8.4 Hz, 1H), 7.61 (d, J = 1.5 Hz, 1H), 7.42 (dd, J = 8.4, 1.5 Hz, 1H), 4.43-4.41 (m, 4H), 2.06 (s, 3H). AH-3 [00256] ESI-MS m/z = 310.0 [M + H]+ AH-4 [00257]

AI: 3-(6-bromo-1H-indazol-1-yl)-2,2-dimethylpropan-1-ol

[0632] ##STR00258##

[0633] Step A

[0634] A mixture of (3-bromo-2,2-dimethylpropoxy)(tert-butyl)dimethylsilane (3.7 g, 18.9 mmol, 1.0 equiv), 6-bromo-1H-indazole (7.9 mg, 28.3 mmol, 1.5 equiv), K.sub.2CO.sub.3 (5.2 mg, 37.8 mmol, 2.0 equiv) and KI (6.3 g, 37.8 mmol, 2.0 equiv) in DMF (20 mL) was stirred at 150 C. for 24 hours. The mixture was diluted with ethyl acetate (100 mL), then washed with water (250 mL) and brine (80 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was purified by silica gel chromatography (ethyl acetate/petroleum ether (1:10)) to give 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indazole (6.3 g, 84% yield) as a brown oil. ESI-MS m/z=397.2[M+H].sup.+.

[0635] Step B

[0636] TBAF (8.8 g, 33.8 mmol, 2.0 eq, 1.0 M in THF) was add to a solution of 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indazole (6.7 g, 16.9 mmol, 1.0 equiv) in THF (40 mL), and then the mixture was stirred at 20 C. for 6 hours. The mixture was diluted with EA (100 mL), then washed with water (20 mL6) and brine (80 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue that was purified by silica gel chromatography (ethyl acetate/petroleum ether (1:5)) to give 3-(6-bromo-1H-indazol-1-yl)-2,2-dimethylpropan-1-ol (2.1 g, 44% yield) as a brown oil. ESI-MS m/z=283.1 [M+H].sup.+. .sup.1H-NMR (400 MHz, DMSO-d.sub.6) 8.10 (d, 1H), 8.03 (s, 1H), 7.70 (s, 1H), 7.24 (m, 1H), 4.79 (s, 1H), 4.24 (s, 2H), 3.16 (d, 2H), 0.84 (s, 6H).

[0637] The following intermediates were synthesized according to Intermediate AI described above using appropriate building blocks and modified reaction conditions (such as ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00015 Intermediate No. Structure Analytical Data AI-1 [00259] ESI-MS m/z = 317.0[M + H]+ AI-2 [00260] ESI-MS m/z = 323.1 [M + H]+. AI-3 [00261] ESI-MS m/z = 308.1 [M + H]+. AI-4 [00262] ESI-MS m/z = 411.2 [M + H]+. AI-5 [00263] ESI-MS m/z = 351.0 [M + H]+ AI-6 [00264] H NMR (300 MHz, CDCl3) 7.55-7.36 (m, 2H), 7.12 (s, 1H), 4.03 (s, 3H), 3.96 (s, 2H), 3.28 (s, 2H), 0.96 (s, 6H) AI-7 [00265] ESI-MS m/z = 350.2 [M + H]+ 1H-NMR (400 MHz, DMSO-d.sub.6) = 8.08 (s, 1H), 7.89 (d, J = 8.7 Hz, 1H), 7.34 (dd, J = 8.7, 1.6 Hz. 1H), 4.80-4.75 (m, 1H), 4.23 (s, 2H), 3.16 (d, J = 5.2 Hz, 2H), 1.84 (d, J = 1.4 Hz, 6H), 0.85 (d, J = 1.4 Hz, 6H).

AJ: (S)-3-(6-bromo-3-(difluoromethyl)-1H-indazol-1-yl)-2-methylpropan-1-ol

[0638] ##STR00266##

[0639] Step A

[0640] To a 100 mL flask was added 6-bromo-1H-indazole-3-carbaldehyde (2.25 g, 10 mmol, 1 equiv) in DMSO (30 mL) was added (R)-(3-bromo-2-methylpropoxy)(tert-butyl)diphenylsilane (5.0 g, 13 mmol, 1.3 equiv), KI (1.66 g, 10 mmol, 1 equiv), and K.sub.2CO.sub.3 (2.76 g, 20 mmol, 2 equiv). The mixture was stirred at 150 C. for 16 hours. The mixture was cooled and poured into water (150 mL) and extracted with ethyl acetate (325 mL). The combined organic layers were washed with H.sub.2O (50 mL), brine (325 mL), then dried over sodium sulfate and filtered. After concentration, the residue was purified by silica gel chromatography (petroleum ether) to afford (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indazole-3-carbaldehyde (4.3 g, 80% yield) as a colorless oil. .sup.1H NMR (400 MHz. CDCl.sub.3) 10.18 (s, 1H), 8.17 (d, J=8.6 Hz, 1H), 7.75 (s, 1H), 7.63 (ddd, J=15.5, 7.9, 1.3 Hz, 4H), 7.527.29 (m, 7H), 4.65 (dd, J=13.8, 6.5 Hz, 1H), 4.28 (dd, J=13.8, 7.3 Hz, 1H), 3.61-3.49 (m, 2H), 2.40 (tp, J=13.4, 6.8 Hz, 1H), 1.12 (s, 9H), 0.95 (d, J=6.9 Hz, 3H).

[0641] Step B

[0642] To a solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indazole-3-carbaldehyde (3.6 g, 6.74 mmol, 1.0 equiv) in dichloromethane (30 mL) was added DAST (15 mL) at 20 C. The solution was stirred at room temperature for 16 hours. The reaction was poured into ice water and extracted with ethyl acetate (330 mL). The organic layers were washed with water, brine, and dried over sodium sulfate. After filtration and concentration, the residue was purified by silica gel chromatography (petroleum/ethyl acetate (10:1)) to afford (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-(difluoromethyl)-1H-indazole (2.1 g, 56% yield) as a colorless oil. ESI-MS m/z=557.1 [M+H].sup.+.

[0643] Step C

[0644] To a solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-(difluoromethyl)-1H-indazole (2.1 g, 3.78 mmol, 1.0 equiv) in THF (15 mL) was added TBAF (1 M in THF, 3.8 mL, 3.8 mmol, 1.0 equiv) at 20 C. The solution was stirred at room temperature for 0.5 hours. After concentration, the crude product was diluted with ethyl acetate (20 mL) and washed with water (55 mL). The organic layer was dried over anhydrous sodium sulfate and filtered. After concentration, the residue was purified by column chromatography (petroleum/ethyl acetate (1:1)) to afford 3-(6-bromo-3-(difluoromethyl)-1H-indazol-1-yl)-2-methylpropan-1-ol (1.0 g, 84% yield) as a white solid.

AK: (S)-3-(6-bromo-3-(methoxymethyl)-1H-Indazol-1-yl)-2-methylpropan-1-ol

[0645] ##STR00267##

[0646] Step A

[0647] To a stirred solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indazole-3-carbaldehyde (5.0 g, 9.3 mmol, 1.0 equiv) in MeOH (25 mL) and THF (50 mL) was added NaBH.sub.4 (700 mg, 18.7 mmol, 2.0 equiv) at 15 C. The mixture was stirred at 15 C. for 2 hours. The solution was poured into water (300 mL) and extracted with ethyl acetate (150 mL3). The combined organic layers were washed with water (200 mL2) and brine (200 mL1), dried over sodium sulfate and concentrated to give a residue. The residue was purified by silica gel chromatography (petroleum ether/ethyl acetate (5:1)) to give (S)-(6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indazol-3-yl)methanol (4.3 g, 86% yield) as an oil. ESI-MS m/z=537.3 [M+H].sup.+.

[0648] Step B

[0649] (S)-(6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indazol-3-yl)methanol (500 mg, 0.93 mmol, 1.0 equiv) was dissolved in THF (10 mL) at 0 C., and NaH (60% dispersion in oil, 74 mg. 1.86 mmol, 2.0 equiv) was added in portions. The mixture was stirred at 0 C. for 0.5 hours, then MeI (264 mg, 1.86 mmol, 2.0 equiv) was added. The reaction mixture was stirred at 0 C. to 15 C. for 16 hours, and then the reaction mixture was poured into 50 mL of ice water. The solution was extracted with ethyl acetate (30 mL3). The organic phase was concentrated to give a residue. The residue was purified by silica gel chromatography (petroleum ether/ethyl acetate (10:1)) to give (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-(methoxymethyl)-1H-indazole (400 mg, 78% yield) as an oil. ESI-MS m/z=551.3 [M+H].sup.+. .sup.1H-NMR (400 MHz, CDCl.sub.3) 7.70-7.57 (m, 6H), 7.45-7.31 (m, 6H), 7.27-7.23 (m, 1H), 4.77 (s, 2H), 4.50 (dd, J=14.0, 6.6 Hz, 1H), 4.19-4.13 (m, 1H), 3.51 (qd, J=10.3, 5.1 Hz, 2H), 3.37 (s, 3H), 2.34 (dt, J=12.1, 6.2 Hz, 1H), 1.11 (s, 9H), 0.91 (d, J=6.9 Hz, 3H).

[0650] Step C

[0651] To a stirred solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-(methoxymethyl)-1H-indazole (4.5 g, 8.2 mmol, 1.0 equiv) in THF (30 mL) was added TBAF (1 M in THF, 16.4 mL, 16.4 mmol, 2.0 equiv) at 0 C. The resulting mixture was stirred for 2 hours at 0 C. After concentration, the crude product was diluted with ethyl acetate (250 mL) and washed with water (30 mL5). The organic layer was concentrated under reduced pressure to give a residue that was purified by flash column on silica gel (petroleum/ethyl acetate=3:1) to give (S)-3-(6-bromo-3-(methoxymethyl)-1H-indazol-1-yl)-2-methylpropan-1-ol (2.5 g, 88% yield) as an oil. ESI-MS m/z=313.0 [M+H].sup.+.

AL: 3-(6-bromo-3-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-1H-indazol-1-yl)-2,2-dimethylpropan-1-ol

[0652] ##STR00268##

[0653] Into a 40 mL vial was placed 3-(6-bromo-3-iodo-1H-indazol-1-yl)-2,2-dimethylpropan-1-ol (700 mg, 1.71 mmol, 1 equiv), 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,2,3,6-tetrahydropyridine (458.2 mg, 2.05 mmol, 1.2 equiv), K.sub.2CO.sub.3 (591.2 mg, 4.28 mmol, 2.5 equiv), Pd(dppf)Cl.sub.2 (150 mg, 0.21 mmol, 0.12 equiv), and dioxane/H.sub.2O (10 mL). The resulting solution was stirred for 2 hours at 60 C. The solids were filtered off and the resulting mixture was concentrated. The residue purified by silica gel chromatography with dichloromethane/methanol (15:1) to give 3-(6-bromo-3-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-1H-indazol-1-yl)-2,2-dimethylpropan-1-ol (634 mg, 93% yield) as a dark yellow oil. ESI-MS m/z=378.0 [M+H].sup.+.

AM: (S)-3-(6-bromo-3-(prop-1-yn-1-yl)-1H-Indazol-1-yl)-2-methylpropan-1-ol

[0654] ##STR00269##

[0655] To a stirred solution of (2S)-3-(6-bromo-3-iodo-1H-indazol-1-yl)-2-methylpropan-1-ol (1.5 g, 3.80 mmol, 1 equiv) and tributyl(prop-1-yn-1-yl)stannane (1.38 g, 4.18 mmol, 1.1 equiv) in THF was added Pd(PPh.sub.3).sub.4 (439 mg, 0.38 mmol, 0.1 equiv) and LiCl (483 mg, 11.4 mmol, 3.0 equiv) in portions. The resulting mixture was stirred for 2 hours and concentrated under vacuum. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (3:1) to afford (2S)-3-[6-bromo-3-(prop-1-yn-1-yl)-1H-indazol-1-yl]-2-methylpropan-1-ol (680 mg, 53% yield) as a yellow oil. ESI-MS m/z=307.1 [M+H].sup.+.

AN: 4-(6-bromo-1(3-hydroxy-2,2-dimethylpropyl)-1H-indazol-3-yl)-2-methylbut-3-yn-2-ol

[0656] ##STR00270##

[0657] A solution of 3-(6-bromo-3-iodo-1H-indazol-1-yl)-2,2-dimethylpropan-1-ol (470 mg, 1.15 mmol, 1 equiv) in THF (16 mL) at 0 C. was treated with Et.sub.3N (4 mL), 2-methylbut-3-yn-2-ol (116 mg, 1.38 mmol, 1.20 equiv), CuI (23 mg, 0.11 mmol, 0.10 equiv), and PdCl.sub.2(PPh.sub.3).sub.2 (120.6 mg, 0.17 mmol, 0.15 equiv). The solution was stirred for 2 hours at 0 C., and then concentrated. The residue was diluted with 20 mL of water and then extracted with ethyl acetate (240 mL). The organics were washed with 30 mL of water. The organics were dried over sodium sulfate, filtered, and the solvent was removed in vacuo. The residue was purified by silica gel chromatography eluting with ethyl acetate/petroleum ether (1:1) to give 4-(6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indazol-3-yl)-2-methylbut-3-yn-2-ol (88% yield) as a dark yellow oil. ESI-MS n/z=365.1 [M+H].sup.+.

AO: 3-(6-bromo-3-((trimethylsilyl)ethynyl) 1H-Indazol-1-yl-2,2-dimethylpropan-1-ol

[0658] ##STR00271##

[0659] The title compound was synthesized using a procedure similar to the one described for the synthesis of 4-(6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indazol-3-yl)-2-methylbut-3-yn-2-ol to give the desired product in 83% yield. ESI-MS m/z=379.1 [M+H].sup.+.

AP: 6-bromo-1-(2-(hydroxymethyl)allyl)-1H-indole-3-carbonitrile

[0660] ##STR00272##

[0661] To a stirred solution of 6-bromo-1H-indole-3-carbonitrile (1.0 g, 4.5 mmol, 1.0 equiv) in DMF (30 mL) was added 2-(bromomethyl)prop-2-en-1-ol (910 mg, 6.0 mmol, 1.5 equiv) followed by K.sub.2CO.sub.3 (1.2 g, 9.0 mmol, 2.0 equiv). The mixture was stirred at 70 C. for 16 hours. The solution was then poured into water (200 mL), extracted with ethyl acetate (100 mL3). The combined organic layer was washed with water (2100 mL) and brine (100 mL) and dried over sodium sulfate and concentrated to give a crude residue that was purified by silica gel chromatography (petroleum ether/ethyl acetate (5:1)) to give 6-bromo-1-(2-(hydroxymethyl)allyl)-1H-indole-3-carbonitrile (1.1 g, 78% yield) as an oil. ESI-MS m/z=291.0 [M+H].sup.+.

[0662] The following intermediates were synthesized according to the procedure described to make Intermediate AP using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00016 Intermediate No. Structure Analytical Data AP-1 [00273] AP-2 [00274] ESI-MS m/z = 283.1 [M + H]+ AP-3 [00275] ESI-MS m/z = 269.1 [M + H]+ AP-4 [00276] ESI-MS m/z = 296.1[M + H]+ AP-5 [00277] ESI-MS m/z = 255.2; 257.2 [M + H]+; .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 8.38 (s, 1H), 8.24 (s, 1H), 7.70 (s, 1H), 8.59 (s, 1H), 4.64-4.61 (m, 1H), 4.28-4.23 (m, 2H), 3.31 (m, 2H), 1.93-1.84 (m, 2H). AP-6 [00278] ESI-MS m/z = 285.0

AQ: (S)-6-bromo-1-(3-hydroxy-2-methylpropyl)-3,3-dimethylindolin-2-one

[0663] ##STR00279##

[0664] A solution of 6-bromo-3,3-dimethyl-2,3-dihydro-1H-indol-2-one (800 mg, 3.33 mmol, 1.0 equiv), DMF (10 mL), (2R)-3-bromo-2-methylpropan-1-ol (560.8 mg, 3.67 mmol, 1.1 equiv) and Cs.sub.2CO.sub.3 (3.26 grams, 10.00 mmol, 3.0 equiv) was stirred for 15 hours at room temperature. The residue was purified by silica gel chromatography (petroleum ether/ethyl acetate (2:1)) to afford (S)-6-bromo-1-(3-hydroxy-2-methylpropyl)-3,3-dimethylindolin-2-one (850 mg, 82% yield) as a yellow oil.

[0665] The following intermediate were synthesized according to Intermediate AQ described above using appropriate building blocks and modified reaction conditions (such as ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00017 Intermediate No. Structure Analytical Data AQ-1 [00280] ES1-MS m/z = 354.1 [M + H]+

AR: 2-(6-bromoquinolin-4-yl)ethan-1-ol

[0666] ##STR00281##

[0667] Step A

[0668] To a solution of tert-butyl methyl malonate (6.5 g, 0.0374 mol, 3.0 equiv) in DMF was added sodium hydride (1.0 g, 0.0436 mol. 3.5 equiv). The mixture was stirred for 1 hour at 80 C., and then cooled to room temperature and 6-bromo-4-chloroquinoline (3 g, 0.0124 mol, 1.0 equiv) was added. The reaction was stirred for 15 hours at 100 C. The reaction was treated with NaHSO.sub.4 (10% aqueous) and then extracted with ethyl acetate (450 mL), washed with brine, and dried over sodium sulfate. After filtration, the solution was concentrated and purified by silica gel chromatography (petroleum ether/ethyl acetate (1:4)) to give 1-(tert-butyl) 3-methyl 2-(6-bromoquinolin-4-yl)malonate (2.7 g, 57% yield) as a yellow oil. ESI-MS m/z=380.1 [M+H].sup.+.

[0669] Step B

[0670] To a solution of 1-(tert-butyl) 3-methyl 2-(6-bromoquinolin-4-yl)malonate (2.7 g, 0.0071 mol, 1.0 equiv) in dichloromethane was added TFA (10 ml) at 0 C. The mixture was stirred for 6 hours at room temperature. Water was added and the solution was neutralized to pH 7. After extraction with ethyl acetate (3), the organics were washed with brine, dried over sodium sulfate, and filtered. Finally, the organic phase was concentrated and purified by silica gel chromatography (petroleum ether/ethyl acetate (1:4)) to give methyl 2-(6-bromoquinolin-4-yl)acetate (600 mg, 30% yield) as a yellow solid. ESI-MS m/z=280.1 [M+H].sup.+.

[0671] Step C

[0672] To a solution of methyl 2-(6-bromoquinolin-4-yl)acetate (600 mg, 0.0021 mol, 1.0 equiv) in THF at 0 C. was added lithium aluminum hydride (163 mg, 0.0042 mol, 2.0 equiv) portionwise. The mixture was stirred overnight at room temperature and then water was added the solution was extracted with ethyl acetate. The organic phase was concentrated and purified by Prep-HPLC to afford 2-(6-bromoquinolin-4-yl)ethan-1-ol (220 mg, 41% yield) as a yellow oil. ESI-MS m/z=252.0 [M+H].sup.+.

AS: 3-(6-bromo-2,3-dihydro-4H-benzo[b][1,4]oxazin-4-yl)propan-1-ol

[0673] ##STR00282##

[0674] Step A

[0675] To a stirred solution of 6-bromo-3,4-dihydro-2H-benzo[b][1,4]oxazine (9.0 g, 42.0 mmol, 1.0 equiv) in DMF (90.0 mL) was added NaH (60% dispersion, 2.5 g, 62.5 mmol, 1.5 equiv) at 0 C. The mixture was stirred for 1 hour and then (3-bromopropoxy)(tert-butyl)dimethylsilane (16.0 g, 62.5 mmol, 1.5 equiv) was added. The resulting mixture was stirred for 16 hours at 20 C. The solution was diluted with ethyl acetate (700 mL) and water (700 mL). The layers were separated and the organic layer was washed with water (3300 mL), brine (200 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure to give a crude residue. The residue was purified by silica gel chromatography (petroleum ether) to give the 6-bromo-4-(3-((tert-butyldimethylsilyl)oxy)propyl)-3,4-dihydro-2H-benzo[b][1,4]oxazine (9.5 g, 59% yield). ESI-MS m/z=386.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl3) 6.78 (d, J=2.1 Hz, 1H), 6.65 (d, J=2.1 Hz, 1H), 6.61 (d, J=8.4 Hz, 1H), 4.20 (dd, J=9.0, 4.5 Hz, 2H), 3.72-3.65 (m, 4H), 3.38-3.30 (m, 4H), 0.92 (d, J=3.1 Hz, 9H), 0.07 (d, J=3.2 Hz, 6H).

[0676] Step B

[0677] To a stirred solution of 6-bromo-4-(3-((tert-butyldimethylsilyl)oxy)propyl)-3,4-dihydro-2H-benzo[b][1,4]oxazine (500 mg, 1.29 mmol, 1.0 equiv) in MeOH (0.5 mL) was added HCl/MeOH (10 N, 5.0 mL) at 20 C. The resulting mixture was stirred for 0.5 hours at 20 C. The solution was concentrated under reduced pressure to give a residue that was diluted with ethyl acetate (20 mL) and washed with aqueous sodium bicarbonate solution (20 mL). The organic phase was dried over anhydrous sodium sulfate, then concentrated to give 3-(6-bromo-2,3-dihydro-4H-benzo[b][1,4]oxazin-4-yl)propan-1-ol (250 mg, 71%). ESI-MS m/z=272.0 [M+H].sup.+.

AT: 3-(6-bromo-2,3-dihydro-4H-benzo[b][1,4]oxazin-4-yl)-2-methylpropan-1-ol

[0678] ##STR00283##

[0679] Step A

[0680] To a stirred solution of 6-bromo-3,4-dihydro-2H-benzo[b][1,4]oxazine (7.0 g, 32.7 mmol, 1.0 equiv) in DCE (100 mL) was added 3-((tert-butyldiphenylsilyl)oxy)-2-methylpropanal (10.6 g, 32.7 mmol, 1.0 equiv) and NaBH.sub.3CN (3.1 g, 49.0 mmol, 1.5 equiv) followed by AcOH (2.9 g, 49.0 mmol, 1.5 equiv) at 10 C. The mixture was stirred for 16 hours at 60 C., and then diluted with ethyl acetate (500 mL) and water (500 mL). The layers was separated and the organic layer was washed with water (3300 mL), brine (200 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure to give a residue that was purified by flash column on silica gel (petroleum ether) to give 6-bromo-4-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3,4-dihydro-2H-benzo[b][1,4]oxazine (5.8 g, 34% yield) as an oil. ESI-MS m/z=523.9 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.70-7.62 (m, 4H), 7.47-7.35 (m, 6H), 6.75 (d, J=2.1 Hz, 1H), 6.66 (dd, J=8.4, 2.1 Hz, 1H), 6.61 (d, J=8.4 Hz, 1H), 4.10-3.98 (m, 2H), 3.62 (dd, J=10.1, 4.6 Hz, 1H), 3.53 (dd, J=10.1, 5.5 Hz, 1H), 3.41 (dd, J=14.5, 7.1 Hz, 1H), 3.27 (td, J=5.3, 3.4 Hz, 2H), 2.97 (dd, J=14.4, 7.4 Hz, 1H), 2.11 (dd, J=12.0, 5.2 Hz, 1H), 1.10 (s, 9H), 0.97 (d, J=6.8 Hz, 3H).

[0681] Step B

[0682] To a stirred solution of 6-bromo-4-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3,4-dihydro-2H-benzo[b][1,4]oxazine (5.8 g, 11.0 mmol, 1.0 equiv) in THF (50 mL) was added TBAF (1 M in THF, 28.0 mL, 28.0 mmol, 2.5 equiv) at 0 C. The resulting mixture was stirred for 2 hours at 0 C. After concentration in vacuo, the crude product was diluted with ethyl acetate (50 mL) and washed with water (10 mL5). The organic phase was concentrated under reduced pressure to give a residue that was purified by silica gel chromatography (petroleum ether/ethyl acetate=5:2) to give 3-(6-bromo-2,3-dihydro-4H-benzo[b][1,4]oxazin-4-yl)-2-methylpropan-1-ol (3.2 g, 100% yield) as a white solid. ESI-MS m/z=286.0 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 6.80 (d, J=2.2 Hz, 1H), 6.69 (dd, J=8.4, 2.1 Hz, 1H), 6.63 (d, J=8.4 Hz, 1H), 4.18 (dd, J=6.8, 2.5 Hz, 2H), 3.68-3.56 (m, 2H), 3.37 (dd, J=9.2, 4.7 Hz, 2H), 3.28 (dd, J=14.5, 8.1 Hz, 1H), 3.05 (dd, J=14.5, 6.5 Hz, 1H), 2.14 (ddd, J=13.4, 6.7, 1.3 Hz, 1H), 0.99 (d, J=6.9 Hz, 3H).

AU: (S)-8-bromo-5-ethyl-1-(3-hydroxy-2-methylpropyl)-1,3,4,5-tetrahydro-2H-benzo[b][1,4]diazepin-2-one

[0683] ##STR00284##

[0684] To a stirred solution of (S)-8-bromo-5-ethyl-1-(3-hydroxy-2-methylpropyl)-1,3,4,5-tetrahydro-2H-benzo[b][1,4]diazepin-2-one (2.1 g, 6.154 mmol, 1.0 equiv) in THF was added BH.sub.3.THF (1 N, 25 mL) dropwise at 25 C. under an atmosphere of argon. The resulting mixture was stirred for 1 hour at room temperature and then quenched with 1 mL MeOH. The mixture was neutralized to pH 7 with saturated aqueous NaHCO.sub.3. The resulting mixture was extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (1100 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (10:1 to 5:1) to afford (S)-8-bromo-5-ethyl-1-(3-hydroxy-2-methylpropyl)-1,3,4,5-tetrahydro-2H-benzo[b][1,4]diazepin-2-one (1.6 g, 75% yield) as a colorless oil. ESI-MS m/z=329.1 [M+H].sup.+. .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 6.90-6.81 (m, 2H), 6.65 (d, J=8.4 Hz, 1H), 4.48 (dd, J=5.8, 4.7 Hz, 1H), 4.08-3.96 (m, 1H), 3.38 (dt, J=10.4, 5.2 Hz, 1H), 3.28 (dd, J=10.5, 5.6 Hz, 1H), 3.21-3.00 (m, 7H), 2.84 (dd, J=13.1, 7.4 Hz, 1H), 2.36 (s, 1H), 1.87 (q, J=6.5 Hz, 1H), 1.69 (p, J=6.0 Hz, 2H), 1.08 (t, J=7.0 Hz, 3H), 0.85 (d, J=6.7 Hz, 3H).

AV: (S)-7-bromo-1-ethyl-5-(3-hydroxy-2-methylpropyl)-1,3,4,5-tetrahydro-2H-benzo[b][1,4]diazepin-2-one

[0685] ##STR00285##

[0686] Step A

[0687] To a stirred solution of 7-bromo-2,3,4,5-tetrahydro-1H-1,5-benzodiazepin-2-one (4 g, 16.6 mmol, 1 equiv) in DMF (40 mL) was added NaH (60% dispersion, 0.80 g, 19.9 mmol, 1.2 equiv) in portions at 15 C. under a nitrogen atmosphere. The resulting mixture was stirred for 20 min at 15 C., at which point iodoethane (2.85 g, 18.2 mmol, 1.1 equiv) was added dropwise at 15 C. The resulting mixture was stirred for an additional 2 hours at room temperature. The solution was quenched with saturated aqueous NH.sub.4Cl and the resulting mixture was extracted with ethyl acetate (3150 mL). The combined organic layers were washed with brine (1100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to afford 7-bromo-1-ethyl-2,3,4,5-tetrahydro-1H-1,5-benzodiazepin-2-one (3.5 g, 71% yield) as a yellow solid. .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 7.31-7.11 (m, 2H), 7.05 (dq, J=8.7, 2.0 Hz, 1H), 5.52-5.31 (m, 1H), 3.72 (q, J=7.2 Hz, 2H), 3.55 (tt, J=6.2, 2.7 Hz, 2H), 2.34 (t, J=6.6 Hz, 2H), 0.98 (ddd, J=9.1, 6.5, 2.3 Hz, 3H).

[0688] Step B

[0689] A solution of AcOH (80 mL), 7-bromo-1-ethyl-2,3,4,5-tetrahydro-1H-1,5-benzodiazepin-2-one (800 mg, 2.972 mmol, 1 equiv) and (2R)-3-[(tert-butyldiphenylsilyl)oxy]-2-methylpropanal (1.946 g, 5.960 mmol, 2.01 equiv) was stirred for 10 minutes at room temperature. To the mixture was added NaBH.sub.3CN (282 mg, 4.487 mmol, 1.51 equiv) in portions at room temperature. The resulting mixture was stirred for an additional 16 hours. The resulting mixture was diluted with saturated aqueous NaHCO.sub.3 and then extracted with ethyl acetate (3200 mL). The combined organic layers were washed with brine (150 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to afford 7-bromo-5-[(2S)-3-[(tert-butyldiphenylsilyl)oxy]-2-methylpropyl]-1-ethyl-2,3,4,5-tetrahydro-1H-1,5-benzodiazepin-2-one (2.3 g, crude) as a yellow oil. The crude product was used in the next step directly without further purification. ESI-MS m/z=579.1 [M+H].sup.+.

[0690] Step C

[0691] To a stirred solution of 7-bromo-5-[(2S)-3-[(tert-butyldiphenylsilyl)oxy]-2-methylpropyl]-1-ethyl-2,3,4,5-tetrahydro-1H-1,5-benzodiazepin-2-one (2.3 g, 3.968 mmol, 1.0 equiv) in THF (30 mL) was added TBAF (1 M in THF, 10 mL, 10.0 mmol, 2.52 equiv) dropwise at room temperature. The mixture was stirred for 16 hours at room temperature and then concentrated under reduced pressure. The resulting residue was diluted with water (200 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (1100 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by Prep-TLC (petroleum ether/ethyl acetate (1:4)) to afford 7-bromo-1-ethyl-5-[(2S)-3-hydroxy-2-methylpropyl]-2,3,4,5-tetrahydro-1H-1,5-benzodiazepin-2-one (600 mg, 40% yield) as a yellow solid. .sup.1H-NMR (300 MHz, CDCl.sub.3) 7.27-7.17 (m, 2H), 7.11-7.03 (m, 1H), 3.52 (d, J=5.6 Hz, 4H), 3.16-2.77 (m, 2H), 2.40 (t, J=6.8 Hz, 2H), 2.12-1.88 (m, J=6.0, 5.3 Hz, 1H), 1.41-1.18 (m, 2H), 1.11 (t, J=7.1 Hz, 3H), 0.90 (d, J=6.8 Hz, 3H).

AW: 5-brom 3-hydroxy-2,2-dimethylpropyl)-1-methyl-1,3-dihydro-2H-benzo[d]imidazol-2-one

[0692] ##STR00286##

[0693] Step A

[0694] To a stirred solution of 4-bromo-2-fluoro-1-nitrobenzene (2 g, 9.1 mmol, 1.0 equiv) in DMF (20 mL) was added K.sub.2CO.sub.3 (2.52 g, 18.23 mmol, 2.0 equiv) and 3-amino-2,2-dimethylpropan-1-ol (1.41 g, 13.7 mmol, 1.5 equiv) in portions at 25 C. The resulting mixture was stirred overnight and the resulting mixture was diluted with 150 mL water and extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (1100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (10:1 to 3:1) to afford 3-[(5-bromo-2-nitrophenyl)amino]-2,2-dimethylpropan-1-ol (2.7 g, 96% yield) as a red solid. ESI-MS m/z=303.1 [M+H].sup.+.

[0695] Step B

[0696] A solution of 3-[(5-bromo-2-nitrophenyl)amino]-2,2-dimethylpropan-1-ol (2.7 g, 8.91 mmol, 1 equiv), water (27 mL) and acetic acid (27 mL) was treated with zinc (2.9 g, 44.36 mmol, 4.98 equiv) at 0 C. The resulting solution was stirred for 1 hour while warming to room temperature. The solution was neutralized to pH 7 with saturated aqueous sodium bicarbonate. The resulting mixture was extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (350 mL) and saturated sodium chloride (350 mL). The mixture was dried over anhydrous sodium sulfate and concentrated. The resulting mixture was concentrated under vacuum to give 3-[(2-amino-5-bromophenyl)amino]-2,2-dimethylpropan-1-ol (2.4 g, 91% yield) as a brown solid. ESI-MS m/z=273.1 [M+H].sup.+.

[0697] Step C

[0698] A solution of 3-[(2-amino-5-bromophenyl)amino]-2,2-dimethylpropan-1-ol (2.0 g, 7.32 mmol, 1.0 equiv), N,N-dimethylformamide (20 mL) and imidazole (1.0 g, 14.69 mmol, 2.01 equiv) was treated with tert-butyl(chloro)dimethylsilane (1.2 g, 7.96 mmol, 1.1 equiv) dropwise at 25 C. The resulting solution was stirred for 2 hours at 25 C. The resulting mixture was diluted with 150 mL water and extracted with ethyl acetate (325 mL) and the organic layers were combined. The organics were washed with brine (315 mL). The mixture was dried over anhydrous sodium sulfate and concentrated and the residue was purified by silica gel chromatography eluting with ethyl acetate/petroleum ether (3:8) to give 2.33 g (82% yield) of 5-bromo-N1-[3-[(tert-butyldimethylsilyl)oxy]-2,2-dimethylpropyl]benzene-1,2-diamine as a brown oil. ESI-MS m/z=389.1 [M+H].sup.+.

[0699] Step D

[0700] To a stirred solution of 5-bromo-N-[3-[(tert-butyldimethylsilyl)oxy]-2,2-dimethylpropyl]benzene-1,2-diamine(2.98 g, 7.692 mmol, 1 equiv) in THF (20 mL) was added CDI (1.5 g, 9.25 mmol, 1.2 equiv) in portions at 25 C. The mixture was stirred overnight at 60 C. The resulting mixture was diluted with 150 mL water and extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (1100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (20:1-10:1) to afford 6-bromo-1-[3-[(tert-butyldimethylsilyl)oxy]-2,2-dimethylpropyl]-2,3-dihydro-1H-1,3-benzodiazol-2-one (1.27 g, 37% yield) as a yellow solid. ESI-MS m/z=415.2 [M+H].sup.+.

[0701] Step E

[0702] To a stirred solution of 6-bromo-1-[3-[(tert-butyldimethylsilyl)oxy]-2,2-dimethylpropyl]-2,3-dihydro-1H-1,3-benzodiazol-2-one (1.27 g, 3.072 mmol, 1.0 equiv) in N,N-dimethylformamide (10 mL) was added cesium carbonate (2.0 g, 6.12 mmol, 2.0 equiv) and iodomethane (0.86 g, 6.06 mmol, 1.97 equiv) dropwise at 0 C. The resulting mixture was stirred overnight after warming to room temperature. The resulting mixture was diluted with 100 mL of water and extracted with ethyl acetate (350 mL). The combined organic layers were washed with water (250 mL) and brine (1100 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (20:1 to 6:1) to afford 5-bromo-3-[3-[(tert-butyldimethylsilyl)oxy]-2,2-dimethylpropyl]-1-methyl-2,3-dihydro-1H-1,3-benzodiazol-2-one (1.26 g, 92% yield) as a white solid. ESI-MS m/z=429.2 [M+H].sup.+.

[0703] Step F

[0704] To a stirred solution of 5-bromo-3-[3-[(tert-butyidimethylsilyl)oxy]-2,2-dimethylpropyl]-1-methyl-2,3-dihydro-1H-1,3-benzodiazol-2-one (1.23 g, 2.877 mmol, 1.0 equiv) in THF (10 mL) was added TBAF (1 N in THF, 3.4 mL) dropwise at 0 C. The resulting mixture was stirred overnight at room temperature and then concentrated under vacuum. The residue was purified by Prep-TLC (petroleum ether/ethyl acetate (1:2)) to afford 5-bromo-3-(3-hydroxy-2,2-dimethylpropyl)-1-methyl-2,3-dihydro-1H-1,3-benzodiazol-2-one (875 mg, 94% yield) as a white solid. ESI-MS m/z=315.1 [M+H].sup.+.

AX: (S)-5-bromo-1-cyclobutyl-3-(3-hydroxy-2-methy/propyl)-1,3-dihydro-2H-benzo[d]imidazol-2-one

[0705] ##STR00287##

[0706] Step A

[0707] A solution of 4-bromo-1-fluoro-2-nitrobenzene (3.00 g, 13.6 mmol, 1.0 equiv), cyclobutanamine (1.16 g, 16.4 mmol, 1.2 equiv) and K.sub.2CO.sub.3 (5.65 g, 40.9 mmol, 3.0 equiv) in DMF (60 mL) was stirred for 2 hours at 25 C. The reaction cooled to 0 C., and then water was added. The resulting mixture was extracted with ethyl acetate (3250 mL). The combined organic layers were washed with water (3250 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (5:1) to give 4-bromo-N-cyclobutyl-2-nitroaniline (3.7 g, 90.07% yield) as a red oil. ESI-MS m/z=271.1 [M+H].sup.+.

[0708] Step B

[0709] A solution of 4-bromo-N-cyclobutyl-2-nitroaniline (3.70 g, 13.7 mmol, 1.0 equiv) and zinc (4.46 g, 68.2 mmol, 5.0 equiv) in AcOH (40 ml) and H.sub.2O (40 mL) was stirred for 1 hour at room temperature. The precipitated solids were collected by filtration and washed with AcOH (310 mL). The filtrate was basified to pH 9 with 1 N NaOH. The solution was extracted with ethyl acetate (3100 mL). The combined organic layers were washed with water (3100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to afford 4-bromo-N1-cyclobutylbenzene-1,2-diamine (2.7 g, 82% yield) as a red oil. ESI-MS m/z=241.3 [M+H].sup.+.

[0710] Step C

[0711] A solution of 4-bromo-N-cyclobutylbenzene-1,2-diamine (2.70 g, 11.2 mmol, 1.0 equiv) and CDI (3.63 g, 22.4 mmol, 2.0 equiv) in THF (30 ml) was stirred for 15 hours at 60 C. The resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (1:1) to afford 5-bromo-1-cyclobutyl-1,3-dihydro-2H-benzo[d]imidazol-2-one (2.4 g, 80% yield) as a brown solid. ES-MS m/z=267.0 [M+H].sup.+.

[0712] Step D

[0713] A solution of 5-bromo-1-cyclobutyl-2,3-dihydro-1H-1,3-benzodiazol-2-one (2.40 g, 8.99 mmol, 1.0 equiv), (2R)-3-bromo-2-methylpropan-1-ol (2.06 g, 13.5 mmol, 1.5 equiv), and Cs.sub.2CO.sub.3 (5.85 g, 18.0 mmol, 2.0 equiv) in DMF (30 mL) was stirred for 5 hours at room temperature. The resulting mixture was extracted with ethyl acetate (3250 mL). The combined organic layers were washed with water (3250 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (2:1) to afford 5-bromo-1-cyclobutyl-3-[(2S)-3-hydroxy-2-methylpropyl]-2,3-dihydro-1H-1,3-benzodiazol-2-one (3.0 g, 98% yield) as a brown oil. ESI-MS m/z=339.3 [M+H].sup.+.

[0714] The following intermediates were synthesized according to Intermediate AX described above using appropriate building blocks and modified reaction conditions (such as ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00018 Intermediate No. Structure Analytical Data AX-1 [00288] ESI-MS m/z = 325.0, 327.0 [M + H]+ AX-2 [00289] ESI-MS m/z = 349.3 [M + H]+ AX-3 [00290] ESI-MS m/z = 367.2 [M + H] AX-4 [00291] ESI-MS m/z = 371.2 [M + H]+; 1H-NMR (300 MHz, DMSO-d.sub.6) 7.44 (d, J = 2.2 Hz, 1H), 7.30 (d, J = 8.2 Hz, 1H), 7.20 (dt, J = 8.7, 2.1 Hz, 1H), 4.63 (t, J = 4.8 Hz, 1H), 4.55-4.32 (m, 1H), 4.10-3.87 (m, 2H), 3.85-3.61 (m, 2H), 3.47 (t, J = 11.8 Hz, 2H), 3.30 (t, J = 5.5 Hz, 2H), 2.44-2.15 (m, 2H), 2.03 (dd, J = 12.8, 6.3 Hz, 1H), 1.73-1.49 (m, 2H), 0.82 (d, J = 6.7 Hz, 3H).

AY: (S)-5-bromo-3-(3-hydroxy-2-methylpropyl)-1-(pyridin-4-yl)-1,3-dihydro-2H-benzo[d]imidazol-2-one

[0715] ##STR00292##

[0716] Step A

[0717] A solution of (R)-(3-bromo-2-methylpropoxy)(tert-butyl)diphenylsilane (3.75 g, 9.6 mmol, 1.0 equiv), Cs.sub.2CO.sub.3 (6.24 g, 19.2 mmol, 2.0 equiv), and NH(Boc).sub.2 (2.3 g, 10.6 mmol, 1.1 equiv) in DMF (20 mL) was stirred at 80 C. for 3 hours. The solution was poured into ice water (200 mL) and extracted with ethyl acetate (3100 mL). The combined organic layer was dried over anhydrous sodium sulfate and concentrated under reduced pressure to give a crude product, which was purified by silica gel chromatography (petroleum ether to petroleum ether/ethyl acetate (5:1)) to give the desired product (4.5 g, 85%) as a clear oil. ESI-MS m/z=550.3 [M+Na].sup.+.

[0718] Step B

[0719] To a solution of starting amine (4.5 g, 10.5 mmol, 1.0 equiv) in dichloromethane (12 mL) was added TFA (4 mL) dropwise. The mixture was stirred at for 3 hours and then was concentrated to give (S)-3-((tert-butyldiphenylsilyl)oxy)-2-methylpropan-1-amine. The crude product was used for the next step directly without further purification. ESI-MS m/z=328.3 [M+H].sup.+.

[0720] Step C

[0721] A solution of 4-bromo-2-fluoro-1-nitrobenzene (2.31 g, 10.5 mmol, 1.0 equiv), K.sub.2CO.sub.3 (2.9 g, 21.0 mmol, 2.0 equiv), and (S)-3-((tert-butyldiphenylsilyl)oxy)-2-methylpropan-1-amine (TFA salt, 4.63 g) was stirred, at which point water (100 mL) was added and the solution was extracted with ethyl acetate (3100 mL). The combined organic layer was dried over anhydrous sodium sulfate and concentrated under reduced pressure to give a crude product, which was purified by silica gel chromatography (petroleum ether to petroleum ether/ethyl acetate (3:1)) to give (S)-5-bromo-N-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-2-nitroaniline (3.15 g, 70%) as a yellow oil. ESI-MS m/z=527.2 [M+H].sup.+.

[0722] Step D

[0723] To a stirred solution of (S)-5-bromo-N-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-2-nitroaniline (3.15 g, 5.97 mmol, 1.0 equiv) in EtOH (100 mL) was added iron (3.34 g, 59.7 mmol, 10.0 equiv) and NH.sub.4Cl (3.2 g, 59.7 mmol, 10.0 equiv) as a solution in H.sub.2O (100 mL). The reaction was then stirred at 90 C. for 2 hours. The mixture was cooled to room temperature and then poured into water and extracted with ethyl acetate (100 mL3). The organic layer was dried over anhydrous sodium sulfate, and concentrated under vacuum to give the crude (S)-5-bromo-N.sub.1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)benzene-1,2-diamine (2.55 g, 86% yield), which was used for the next step. ESI-MS m/z=497.2 [M+H].sup.+.

[0724] Step E

[0725] A solution of (S)-5-bromo-N1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)benzene-1,2-diamine (2.35 g, 4.7 mmol, 1.0 equiv) and CDI (2.28 g, 14.1 mmol, 3.0 equiv) in THF (30 mL) was stirred at 70 C. for 16 hours. The reaction mixture was cooled to room temperature and poured into water (100 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure to give the crude product, which was purified by silica gel chromatography (petroleum ether to petroleum ether/ethyl acetate (1:1)) to give (S)-6-bromo-1-(3-((tert-butyidiphenylsilyl)oxy)-2-methylpropyl)-1,3-dihydro-2H-benzo[d]imidazol-2-one (1.56 g, 63% yield) as a white solid. ESI-MS m/z=523.1 [M+H].sup.+.

[0726] Step F

[0727] A solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1,3-dihydro-2H-benzo[d]imidazol-2-one (1.52 g, 2.9 mmol, 1.0 equiv), CuI (55 mg, 0.29 mmol, 0.1 equiv), 4-iodopyridine (1.19 g, 5.8 mmol, 1.0 equiv), K.sub.2CO.sub.3 (1.2 g, 8.7 mmol, 3.0 equiv), and N1,N1-dimethylethane-1,2-diamine (51 mg, 0.58 mmol, 0.2 equiv) in dioxane (20 mL) was stirred at 110 C. for 5 hours. The reaction was quenched by addition of ice water (100 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure to give a crude product, which was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH (50:1)) to give (S)-5-bromo-3-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1-(pyridin-4-yl)-1,3-dihydro-2H-benzo[d]imidazol-2-one (1.37 g, 78% yield) as a white solid.

[0728] Step G

[0729] To a solution of (S)-5-bromo-3-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1-(pyridin-4-yl)-1,3-dihydro-2H-benzo[d]imidazol-2-one (1.37 g, 2.28 mmol, 1.0 equiv) in THF (15 mL) was added TBAF (1N in THF, 4.56 mL, 1 M) dropwise at 20 C. The mixture was stirred at 20 C. for 5 hours and then poured into water and extracted with ethyl acetate (100 mL). The organic layer was washed with brine (50 mL3) and dried over anhydrous sodium sulfate, filtered, and concentrated to give a crude residue that was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH=20:1) to give (S)-5-bromo-3-(3-hydroxy-2-methylpropyl)-1-(pyridin-4-yl)-1,3-dihydro-2H-benzo[d]imidazol-2-one (215 mg, 60.7% yield) as a white solid. ESI-MS m/z=362.0 [M+H].sup.+.

AZ: (6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indol-3-yl)pyrrolidin-2-one

[0730] ##STR00293##

[0731] Step A

[0732] To a stirred solution of 6-bromo-1H-indole (5.0 g, 25.6 mmol, 1.0 equiv) in DMSO (100 mL) was added (3-bromo-2,2-dimethylpropoxy)(tert-butyl)dimethylsilane (10.7 g, 37.8 mmol, 1.5 equiv) followed by K.sub.2CO.sub.3 (10.7 g, 77.8 mmol, 3.0 equiv) and KI (4.3 g, 25.6 mmol, 1.0 equiv). The mixture was stirred at 120 C. for 16 hours. The solution was poured into water (500 mL) and extracted with ethyl acetate (250 mL3). The combined organic layer was washed with water (300 mL2) and brine (300 mL1), dried over sodium sulfate and concentrated to give a residue. The residue was purified by silica gel chromatography (petroleum ether/ethyl acetate (100:1)) to 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indole (3.4 g, 34% yield) as an oil.

[0733] Step B

[0734] 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indole (2.9 g, 7.3 mmol, 1.0 equiv) was dissolved in DMF (30 mL) at 0 C., and NIS (1.6 g, 7.3 mmol, 1.0 equiv) was added in portions. The reaction mixture was stirred at 0 C. for 1 hour, and then the reaction mixture was poured in 200 mL of ice water and Na.sub.2SO.sub.3 (5.0 g). The organic layer was separated, dried, and was concentrated to give a residue. The residue was purified by silica gel chromatography (petroleum ether) to give 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl-3-iodo-1H-indole (3.2 g, 84% yield) as a white solid. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.58 (s, 1H), 7.25 (d, J=1.4 Hz, 2H), 7.19 (s, 1H), 3.95 (s, 2H), 3.23 (s, 2H), 1.01-0.96 (m, 9H), 0.90 (s, 6H), 0.14-0.10 (m, 6H).

[0735] Step C

[0736] 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-3-iodo-1H-indole (3.2 g, 6.1 mmol, 1.0 equiv) in dioxane (60 mL) was treated with pyrrolidin-2-one (1.0 g, 12.2 mmol, 2.0 equiv), CuI (230 mg, 1.2 mmol, 0.2 equiv), ethylene diamine (72 mg, 1.2 mmol, 0.2 equiv) and Cs.sub.2CO.sub.3 (4.0 mg, 12.2 mmol, 2.0 equiv). The mixture was stirred at 100 C. for 16 hours. After concentration, the residue was purified by silica gel chromatography (Petroleum/ethyl acetate=3/1) to afford 1-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)pyrrolidin-2-one (750 mg, 27% yield) as an oil.

[0737] Step D

[0738] To a stirred solution of 1-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)pyrrolidin-2-one (1.4 g, 2.9 mmol, 1.0 equiv) in THF (18 mL) was added TBAF (1 M in THF, 8.7 mL, 8.7 mmol, 3.0 equiv) at 0 C. The resulting mixture was stirred for 2 hours at 0 C. After concentration, the crude product was diluted with ethyl acetate (50 mL) and washed with water (10 mL5). The organic phase was concentrated under reduced pressure to give a residue that was purified by silica gel chromatography (petroleum/ethyl acetate (2:1)) to give 1-(6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indol-3-yl)pyrrolidin-2-one (790 mg, 75% yield) as an oil. ESI-MS m/z=316.0 [M+H].sup.+. .sup.1H NMR (400 MHz. CDCl.sub.3) 7.60 (d, J=1.5 Hz, 1H), 7.49 (d, J=8.6 Hz, 1H), 7.41 (s, 1H), 7.17 (dd, J=8.6, 1.6 Hz, 1H), 3.98 (t, J=7.0 Hz, 2H), 3.95 (s, 2H), 3.32 (s. 2H), 2.60 (t, J=8.1 Hz, 2H), 2.29-2.19 (m, 2H), 0.96 (s, 6H).

BA: 6-bromo-1-(3-((tert-butyldimethylsilyloxy)-2,2-dimethylpropyl)indolin-2-one

[0739] ##STR00294##

[0740] Step A

[0741] To a stirred solution of 6-bromo-3-chloro-1H-indole (3.0 g, 13.0 mmol, 1.0 equiv) in DMSO (100 mL) was added 3-bromo-2,2-dimethylpropoxy)(tert-butyl)dimethylsilane (8.0 g, 28.6 mmol, 2.2 equiv) followed by K.sub.2CO.sub.3 (5.4 g, 39.1 mmol, 2.0 equiv), and KI (2.2 g, 13.0 mmol, 1.0 equiv). The mixture was stirred at 120 C. for 16 hours. The solution was poured into water (500 mL) and extracted with ethyl acetate (250 mL3). The combined organic layers were washed with water (300 mL2) and brine (300 mL), dried over sodium sulfate and concentrated to give a residue that was purified by silica gel chromatography (petroleum ether/ethyl acetate 100:1) to give 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-3-chloro-1H-indole (5.1 g, 89% yield) as an oil.

[0742] Step B

[0743] A solution of 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-3-chloro-1H-indole (4.0 g, 8.7 mmol, 1.0 equiv) in THF (50 mL) was treated with aqueous 6N HCl (50 mL). The reaction mixture was stirred at 70 C. for 16 hours. The mixture was poured into water (200 mL) and extracted with ethyl acetate (100 mL3), and the organic layers were concentrated to dryness to give a residue. The residue was purified by silica gel chromatography (petroleum/ethyl acetate (10:1)) to afford 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)indolin-2-one (2.0 g, 64% yield) as a yellow solid. ESI-MS m/z=298.0 [M+H].sup.+; .sup.1H-NMR (400 MHz, DMSO-d.sub.6) 7.44 (d, J=1.2 Hz, 1H), 7.23-7.12 (m, 2H), 4.78 (s, 1H), 3.56 (s, 2H), 3.50 (s, 2H), 3.12 (s, 2H), 0.84 (d, J=11.8 Hz, 6H).

BB: Synthesis of 2-(6-bromo-1-(3-hydroxy-2-(hydroxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile

[0744] ##STR00295##

[0745] Step A

[0746] Into a 100 mL tube was added 2-(6-bromo-1H-indol-3-yl)-2-methylpropanenitrile (3.2 g, 12.2 mmol, 1.0 equiv), DMF (40 mL), (2,2-dimethyl-1,3-dioxan-5-yl)methyl methanesulfonate (4.1 g, 18.282 mmol, 1.50 equiv) and Cs.sub.2CO.sub.3 (11.9 g, 36.5 mmol, 3.0 equiv). The resulting mixture was stirred for 16 hours at 50 C., and then diluted with water (400 mL). The resulting mixture was extracted with ethyl acetate (3300 mL). The combined organic layers were washed with water (2200 mL) and brine (50 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (4:1) to afford 2-(6-bromo-1-((2,2-dimethyl-1,3-dioxan-5-yl)methyl)-1H-indol-3-yl)-2-methylpropanenitrile (3.3 g, 62% yield) as a light-yellow solid. ESI-MS m/z=391.1 [M+H].sup.+.

[0747] Step B

[0748] To a stirred solution of 2-(6-bromo-1-((2,2-dimethyl-1,3-dioxan-5-yl)methyl)-1H-indol-3-yl)-2-methylpropanenitrile (3.3 g, 8.433 mmol, 1 equiv) in THF (30 mL) was added concentrated HCl (6 mL) dropwise at 0 C. The resulting mixture was stirred for 3 hours at room temperature. The resulting mixture was concentrated under vacuum and then neutralized to pH 7 with saturated aqueous NaHCO.sub.3. The resulting mixture was extracted with ethyl acetate (3150 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product 2-(6-bromo-1-(3-hydroxy-2-(hydroxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (2.8 g, 85% yield) was used in the next step directly without further purification. ESI-MS m/z=351.0 [M+H].sup.+.

BC: 2-(6-bromo-1-(3-hydroxy-2-(methoxymethyl)propyl)-1H-Indol-3-yl-2-methylpropanenitrile

[0749] ##STR00296##

[0750] Step A

[0751] A solution of 2-[6-bromo-1-[3-hydroxy-2-(hydroxymethyl)propyl]-1H-indol-3-yl]-2-methylpropanenitrile (7.0 g, 19.929 mmol, 1.0 equiv) in THF (70 mL) was treated with NaH (957 mg, 23.9 mmol, 1.2 equiv, 60% dispersion) at 0 C. The resulting mixture was maintained at that temperature for 1 hour and then TBSCl (3.15 g, 20.9 mmol, 1.05 equiv) was added. The resulting solution was stirred for 2 hours at 0 C., and then the reaction was quenched with ice water. The resulting mixture was extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (330 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated and the residue was purified by silica gel chromatography (15% to 30% ethyl acetate in petroleum ether) to give 2-(6-bromo-1-(3-((tert-butyidimethylsilyl)oxy)-2-(hydroxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (6.0 g, 64% yield) of 2-[6-bromo-1-(2-[[(tert-butyldimethylsilyl)oxy]methyl]-3-hydroxypropyl)-1H-indol-3-yl]-2-methylpropanenitrile as an orange oil. ESI-MS m/z=465.2, 467.2 [M+H].sup.+

[0752] Step B

[0753] To a stirred solution of 2-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2-(hydroxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (900 mg, 1.933 mmol, 1 equiv) in THF (10 mL) was added NaH (60% in oil, 69.6 mg, 2.9 mmol, 1.5 equiv) in portions at 0 C. The resulting mixture was stirred for 2 hours at 0 C. To the mixture was added iodomethane (1.372 g, 9.667 mmol, 5 equiv) dropwise over 30 minutes at 0 C. The resulting mixture was stirred for an additional 16 hours at room temperature. The reaction was quenched with water at 0 C., and then further diluted with water (200 mL). The resulting mixture was extracted with ethyl acetate (2200 mL) and the combined organic layers were washed with water (2100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to give 2-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2-(methoxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (700 mg, crude) that was used in the next step directly without further purification.

[0754] Step C

[0755] To a stirred solution of 2-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2-(methoxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (700 mg, 1.460 mmol, 1 equiv) in THF (10 mL) at 0 C. was added TBAF (1.75 mL) dropwise. The resulting mixture was stirred for 1 hour at 0 C. The residue was purified by prep-TLC (EA/PE=1:2) to afford 2-(6-bromo-1-(3-hydroxy-2-(methoxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (437 mg, 74% yield) as a yellow oil. ESI-MS m/z=365.1 [M+H].sup.+.

BD: 3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl-2-methoxypropan-1-ol

[0756] ##STR00297##

[0757] Step A

[0758] A solution of 3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)propane-1,2-diol (1.4 g, 4.91 mmol, 1.0 equiv), TBSCl (1.48 g, 9.820 mmol, 2.0 equiv), and imidazole (1.34 g, 19.6 mmol, 4.0 equiv) in DMF (10 mL) was stirred for 2 hours at room temperature. The reaction was then quenched by the addition of 100 mL of water. The resulting solution was extracted with ethyl acetate (3100 mL), dried over anhydrous sodium sulfate and concentrated. The residue was purified by silica gel chromatography with ethyl acetate/petroleum ether (1:1) to give 1-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)-3-((tert-butyldimethylsilyl)oxy)propan-2-ol (1.0 g, 44% yield) as a yellow solid. ESI-MS m/z=399.2 [M+H].sup.+.

[0759] Step B

[0760] A solution of 1-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)-3-((tert-butyldimethylsilyl)oxy)propan-2-ol (1.0 g, 2.504 mmol, 1.0 equiv) in THF (10 mL) at 5 C. was treated with NaH (0.07 g, 2.754 mmol, 1.1 equiv, 60% dispersion in mineral oil) and maintained at that temperature for 30 min. MeI (0.39 g, 2.748 mmol, 1.10 equiv) was added to the reaction solution and stirred for 30 minutes at 5 C. The reaction was then quenched by the addition of 100 mL of water. The resulting solution was extracted with ethyl acetate (3100 mL) and dried over anhydrous sodium sulfate and concentrated. The residue was purified by silica gel chromatography with ethyl acetate/petroleum ether (1:2) to give 500 mg (38% yield) of 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2-methoxypropyl)-2-methyl-1H-benzo[d]imidazole as a yellow solid. ESI-MS m/z=413.1 [M+H].sup.+.

[0761] Step C

[0762] A solution of 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2-methoxypropyl)-2-methyl-1H-benzo[d]imidazole (500 mg, 1.209 mmol, 1 equiv), and TBAF (1 mL, 1.0 M in THF) in THF (5 mL) was stirred for 1 hour at room temperature. The resulting mixture was concentrated and the residue was purified by silica gel chromatography with ethyl acetate/petroleum ether (1:1) to give 300 mg (64% yield) of 3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)-2-methoxypropan-1-ol as a white solid. ESI-MS m/z=299.0 [M+H].sup.+.

BE: 2-(6-bromo-1-(3-fluoro-2-(hydroxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile

[0763] ##STR00298##

[0764] Step A

[0765] To a stirred solution of 2-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2-(hydroxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (1.7 g, 3.652 mmol, 1 equiv) in dichloromethane (20 mL) was added sodium carbonate (1.5 g, 14.019 mmol, 3.84 equiv) and DAST (2.3 g, 14.269 mmol, 3.91 equiv) in portions at 0 C. The resulting mixture was warmed to room temperature and stirred for 15 hours. The reaction was quenched by the addition of 100 mL of ice water. The resulting mixture was extracted with ethyl acetate (350 mL) and the combined organic layers were washed with brine (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (20:1 to 13:1) to afford 2-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2-(fluoromethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (699 mg, 39% yield) as a yellow oil. ESI-MS m/z=469.0 [M+H].sup.+.

[0766] Step B

[0767] To a stirred solution of 2-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2-(fluoromethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (752 mg, 1.609 mmol, 1.0 equiv) in THF (10 mL) was added TBAF (1 N in THF, 1.9 mL) dropwise at 0 C. The resulting mixture was stirred for 1 hour at 0 C. to 25 C. The residue was concentrated under reduced pressure and purified by prep-TLC (petroleum ether/ethyl acetate 1:1) to afford 2-(6-bromo-1-(3-fluoro-2-(hydroxymethyl)propyl)-1H-indol-3-yl)-2-methylpropanenitrile (522 mg, 90% yield) as a white solid. ESI-MS m/z=355.1 [M+H].sup.+. .sup.1H-NMR (300 MHz. DMSO-d.sub.6) 7.79 (d, J=1.7 Hz, 1H), 7.70 (d, J=8.6 Hz, 1H), 7.41 (s, 1H), 7.27 (dd, J=8.6, 1.7 Hz, 1H), 4.86 (t, J=5.1 Hz, 1H), 4.51 (qd, J=9.3, 4.8 Hz, 1H), 4.35 (qd, J=9.3, 4.9 Hz, 1H), 4.20 (dd, J=7.2, 2.7 Hz. 2H), 3.40 (d, J=7.5 Hz. 2H), 2.40-2.19 (m, 1H), 1.76 (s, 6H).

BF: 2-(6-bromo-1-(3,3-difluoro-2-(hydroxymethyl)propyl)-1H-Indol-3-yl)-2-methylpropanenitrile

[0768] ##STR00299##

[0769] Step A

[0770] A solution of 2-[6-bromo-1-(2-[[(tert-butyldimethylsilyl)oxy]methyl]-3-hydroxypropyl)-1H-indol-3-yl]-2-methylpropanenitrile (2.0 g, 4.30 mmol, 1 equiv), dichloromethane (20 mL) and Dess-Martin periodinane (2.73 g, 6.45 mmol, 1.5 equiv) was stirred for 2 hours at room temperature. The reaction was quenched with aqueous NaHCO.sub.3. The resulting mixture was filtered and the filter cake was washed with ethyl acetate. The resulting mixture was extracted with ethyl acetate (3150 mL) and the combined organic layers were washed with brine and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (2:1) to afford 2-[6-bromo-1-(2-[[(tert-butyldimethylsilyl)oxy]methyl]-3-oxopropyl)-1H-indol-3-yl]-2-methylpropanenitrile (1.5 g, 68% yield) as a yellow oil. ESI-MS m/z=463.1, 465.1 [M+H].sup.+.

[0771] Step B

[0772] A solution of 2-[6-bromo-1-(2-[[(tert-butyldimethylsilyl)oxy]methyl]-3-oxopropyl)-1H-indol-3-yl]-2-methylpropanenitrile (1.5 g, 3.236 mmol, 1 equiv), dichloromethane (15 mL), Na.sub.2CO.sub.3 (1.37 g, 12.945 mmol, 4 equiv) and DAST (3.13 g, 19.418 mmol, 6 equiv) was stirred for 16 hours. The mixture was diluted with water (150 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to afford 2-(6-bromo-1-[3-[(tert-butyldimethylsilyl)oxy]-2-(difluoromethyl)propyl]-1H-indol-3-yl)-2-methylpropanenitrile (1.8 g) as a yellow oil. The crude product was used in the next step directly without further purification. ESI-MS m/z=485.0 [M+H].sup.+.

[0773] Step C

[0774] To a stirred solution of 2-(6-bromo-1-[3-[(tert-butyldimethylsilyl)oxy]-2-(difluoromethyl)propyl]-1H-indol-3-yl)-2-methylpropanenitrile (1.8 g, 3.708 mmol, 1 equiv) in THF (20 mL) was added TBAF (11 mL) dropwise at 0 C. The resulting mixture was stirred for 1 hour at room temperature and then concentrated under reduced pressure. The residue was purified by prep-TLC (PE/ethyl acetate 1:1) to afford 2-[6-bromo-1-[2-(difluoromethyl)-3-hydroxypropyl]-1H-indol-3-yl]-2-methylpropanenitrile (600 mg, 39% yield) as a yellow solid. ESI-MS m/z=371.0 [M+H].sup.+.

BG: 4-(6-bromo-3-(2-cyanopropan-2-yl)-1H-indol-1-yl)-3-(hydroxymethyl)butanenitrile

[0775] ##STR00300##

[0776] Step A

[0777] To a stirred solution of 2-[6-bromo-1-(2-[[(tert-butyldimethylsilyl)oxy]methyl]-3-hydroxypropyl)-1H-indol-3-yl]-2-methylpropanenitrile (1.5 g, 3.222 mmol, 1 equiv) in dichloromethane (20 mL) was added TEA (855 mg, 8.45 mmol, 2.62 equiv) and MsCl (480 mg, 4.190 mmol, 1.30 equiv) dropwise at 0 C. The resulting mixture was stirred for 30 minutes at 0 C. The reaction was quenched with water and the resulting mixture was extracted with ethyl acetate. The combined organic layers were washed brine and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the residue was purified by prep-TLC (PE/ethyl acetate 2:1) to afford 2-[[6-bromo-3-(1-cyano-1-methylethyl)-1H-indol-1-yl]methyl]-3-[(tert-butyldimethylsilyl)oxy]propyl methanesulfonate (1.5 g, 77% yield) as a yellow oil. ESI-MS m/z=565.1, [M+Na].sup.+.

[0778] Step B

[0779] A solution of 2-[[6-bromo-3-(1-cyano-1-methylethyl)-1H-indol-1-yl]methyl]-3-[(tert-butyldimethylsilyl)oxy]propyl methanesulfonate (1.5 g, 2.759 mmol, 1 equiv), DMF (15 mL), H.sub.2O (1.5 mL) and KCN (900 mg, 13.822 mmol, 5.01 equiv) was stirred for 5 hours at 50 C., and then extracted with ethyl acetate. The combined organic layers were washed with brine and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to afford 4-[6-bromo-3-(1-cyano-1-methylethyl)-1H-indol-1-yl]-3-[[(tert-butyldimethylsilyl)oxy]methyl]butanenitrile(1.6 g) as a yellow oil that was carried forward without further purification. ESI-MS m/z=474.2 [M+H].sup.+.

[0780] Step C

[0781] A solution of 4-[6-bromo-3-(1-cyano-1-methylethyl)-1H-indol-1-yl]-3-[[(tert-butyldimethylsilyl)oxy]methyl]butanenitrile (1.6 g, 3.372 mmol, 1 equiv), THF (20 mL) and TBAF (1.0 M in THF, 6.74 mmol, 2 equiv) at room temperature. The resulting mixture was stirred for 1 hour at room temperature and was then concentrated under reduced pressure. The residue was purified by prep-TLC (PE/ethyl acetate 1:2) to afford 3-[[6-bromo-3-(1-cyano-1-methylethyl)-1H-indol-1-yl]methyl]-4-hydroxybutanenitrile (700 mg, 58% yield) as a yellow oil. ESI-MS m/z=360.1 [M+H].sup.+.

BH: 3-(6-bromo-3-(2-cyanopropan-2-yl)-1H-indol-1-yl)-2-chloropropyl Acetate

[0782] ##STR00301##

[0783] Step A

[0784] To a stirred mixture of 2-(6-bromo-1H-indol-3-yl)-2-methylpropanenitrile (1.4 g, 5.34 mmol, 1.0 equiv) in DMF (20 mL) was added (2,2-dimethyl-1,3-dioxolan-4-yl)methyl methanesulfonate (2.3 g, 8.01 mmol, 1.5 equiv), Cs.sub.2CO.sub.3 (4.35 g, 13.35 mmol, 2.5 equiv), and KI (88.6 mg, 0.534 mmol, 0.1 equiv). The reaction was stirred at 45 C. for 48 hours. The resulting mixture was diluted with water (100 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were dried over sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (8:1) to afford 2-(6-bromo-1-((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)-1H-indol-3-yl)-2-methylpropanenitrile (1.7 g, 84% yield) as an yellow oil. ESI-MS m/z=377.3 [M+H].sup.+.

[0785] Step B

[0786] To a stirred mixture of 2-(6-bromo-1-((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)-1H-indol-3-yl)-2-methylpropanenitrile (1.7 g, 4.50 mmol, 1.0 equiv) in THF (10 mL) and water (10 mL) was added TsOH (1.78 g, 10.37 mmol, 2.3 equiv). The reaction was stirred at 30 C. for 15 hours. The resulting mixture was diluted with water (100 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were dried over sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (1:1) to afford 2-(6-bromo-1-(2,3-dihydroxypropyl)-1H-indol-3-yl)-2-methylpropanenitrile (1.4 g, 92% yield) as an yellow oil. ESI-MS m/z=337.1 [M+H].sup.+.

[0787] Step C

[0788] A solution of 3-[6-bromo-3-(1-cyano-1-methylethyl)-1H-indol-1-yl]-2-(methanesulfonyloxy)propyl acetate (120 mg, 0.262 mmol, 1 equiv) and LiCl (111 mg, 2.62 mmol, 10 equiv) in DMF (2 mL) was stirred for 4 hours at 80 C. The reaction was quenched by the addition of 20 mL of water and the resulting solution was extracted with ethyl acetate (320 mL). The organics were dried over anhydrous sodium sulfate, filtered, and concentrated. The residue was purified by silica gel chromatography with ethyl acetate/petroleum ether (1:4) to give 3-[6-bromo-3-(1-cyano-1-methylethyl)-1H-indol-1-yl]-2-chloropropyl acetate (100 mg, 96% yield) as a yellow solid. ESI-MS m/z=397.1/399.1 [M+H].sup.+.

BI: 1-(3-amino-2,2-dimethylpropyl)-6-bromo-1H-indole-3-carbonitrile

[0789] ##STR00302##

[0790] Step A

[0791] DIAD (6.6 g, 32.7 mmol, 2.0 equiv) was added to a solution of 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indole-3-carbonitrile (5.0 g, 16.3 mmol, 1.0 equiv), isoindoline-1,3-dione, and PPh.sub.3 (8.6 g, 32.7 mmol, 2.0 equiv) in THF (100 mL) at 0 C. The resulting solution was stirred 16 hours at room temperature. The mixture was diluted with ethyl acetate (200 mL) and then washed with water (100 mL2) and brine (150 mL). The organic phase was dried over sodium sulfate, filtered, and concentrated to give a residue. The residue was purified by silica gel chromatography (ethyl acetate/petroleum ether=1/5) to give a mixture of 6-bromo-1-(3-(1,3-dioxoisoindolin-2-yl)-2,2-dimethylpropyl)-1H-indole-3-carbonitrile and triphenylphosphine oxide (12.0 g) as a brown oil. ESI-MS m/z=436.0 [M+H].sup.+.

[0792] Step B

[0793] A mixture of 6-bromo-1-(3-(1,3-dioxoisoindolin-2-yl)-2,2-dimethylpropyl)-1H-indole-3-carbonitrile (5.0 g, 11.5 mmol, 1.0 equiv) and hydrazide-hydrate (5.75 g, 115.0 mmol, 10.0 equiv) in EtOH (150 mL) was stirred at 85 C. for 6 hours. The mixture was diluted with ethyl acetate (100 mL) and washed with water (50 mL2) and brine (80 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a crude residue that was purified by silica gel chromatography (ethyl acetate/petroleum ether (2:1)) to give 1-(3-amino-2,2-dimethylpropyl)-6-bromo-1H-indole-3-carbonitrile (3.0 g, 60% yield) as a brown solid. ESI-MS m/z=306.0 [M+H].sup.+.

BJ: 6-bromo-1-(2,2-dimethyl-3-(methylamino)propyl)-1H-indole-3-carbonitrile

[0794] ##STR00303##

[0795] Step A

[0796] A mixture of 1-(3-amino-2,2-dimethylpropyl)-6-bromo-1H-indole-3-carbonitrile (500 mg, 1.64 mmol, 1.0 equiv), Boc.sub.2O (429 mg, 1.968 mmol, 1.2 equiv), and Et.sub.3N (331 mg, 3.28 mmol, 2.0 equiv) in dichloromethane (20 mL) was stirred at room temperature for 16 hours. The mixture was diluted with ethyl acetate (50 mL) and then washed with saturated NaHCO.sub.3 (35 mL), water (50 mL2), and brine (50 mL). The organic phase was collected, dried over sodium sulfate, filtered, and concentrated to give a residue. The residue was purified by silica gel chromatography (ethyl acetate/petroleum ether (1:5)) to give tert-butyl (3-(6-bromo-3-cyano-1H-indol-1-yl)-2,2-dimethylpropyl)carbamate (550 mg, 80% yield) as an off-white solid. ESI-MS m/z=406.1 [M+H].sup.+.

[0797] Step B

[0798] NaH (63 mg, 60% dispersion in mineral oil, 2.62 mmol, 2.0 equiv) was added to a solution of tert-butyl (3-(6-bromo-3-cyano-1H-indol-1-yl)-2,2-dimethylpropyl)carbamate (530 mg, 1.31 mmol, 1.0 equiv) in DMF (10 mL) at 0 C. The mixture was stirred 0.5 hours at 15 C. and then iodomethane (223 mg, 1.57 mmol, 1.2 equiv) was added at 0 C., and the mixture was stirred 16 hours at room temperature. Water (100 uL) was added to the reaction mixture and then the mixture was diluted with ethyl acetate (100 mL) and washed with water (100 mL2) and brine (150 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was purified by silica gel chromatography (ethyl acetate/petroleum ether (1:5)) to give tert-butyl (3-(6-bromo-3-cyano-1H-indol-1-yl)-2,2-dimethylpropyl)(methyl)carbamate (400 mg, 73% yield) as an off-white solid. ESI-MS m/z=420.1 [M+H].sup.+.

[0799] Step C

[0800] tert-Butyl (3-(6-bromo-3-cyano-1H-indol-1-yl)-2,2-dimethylpropyl)(methyl)carbamate (400 mg, 0.95 mmol, 1.0 equiv) was dissolved in methanolic HCl (4 M, 10 mL) and stirred at room temperature for 16 hours. The mixture was concentrated and the residue was purified by silica gel chromatography (ethyl acetate/petroleum ether (2:1)) to give 6-bromo-1-(2,2-dimethyl-3-(methylamino)propyl)-1H-indole-3-carbonitrile (300 mg, 99% yield) as an off-white solid. ESI-MS m/z=320.1 [M+H].sup.+.

BK: 1-(3-hydroxy-2,2-dimethylpropyl)-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indole-3-carbonitrile

[0801] ##STR00304##

[0802] To a solution of 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indole-3-carbonitrile (2.0 g, 6.6 mmol, 1.0 equiv), 4,4,4,4,5,5,5,5-octamethyl-2,2-bi(1,3,2-dioxaborolane) (2.48 g, 9.8 mmol, 1.5 eq), Pd(dppf)Cl.sub.2 (600 mg, 1.98 mmol, 0.3 equiv), and potassium acetate (1.9 g, 19.8 mmol, 3.0 equiv) in dioxane (100 mL) was stirred at 90 C. for 2 hours. After concentration, the mixture was diluted with water (50 mL) and extracted with ethyl acetate (30 mL3). The layers were separated and the organic layer was washed with brine (30 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure. The residue was purified by silica gel chromatography (petroleum/ethyl acetate (5:1 to 3:1)) to afford 1-(3-hydroxy-2,2-dimethylpropyl)-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indole-3-carbonitrile (2.0 g, 87% yield) as a light yellow solid. ESI-MS m/z=355.2 [M+H].sup.+.

[0803] The following intermediates were synthesized according to the procedure described to make Intermediate BK using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00019 Intermediate No. Structure Analytical Data BK-1 [00305] ESI-MS m/z = 341.2 [M + H]+ BK-2 [00306] ESI-MS m/z = 331.3 [M + H]+ BK-3 [00307] ESI-MS m/z = 383.3 [M + H]+

BL: 7-bromo-9-f(2S)-3-hydroxy-2-methyl-propyl-4-methyl-1,3-dihydropyrano[3,4-b]indole-4-Carbonitrile

[0804] ##STR00308##

[0805] Step A

[0806] To a solution of tetrahydropyran-3,5-dione (400 mg, 3.51 mmol, 1.0 equiv) and 5-bromo-2-iodo-aniline (1149 mg, 3.86 mmol, 1.1 equiv) in toluene (11.7 mL) was added PTSA monohydrate (67 mg, 0.35 mmol, 0.1 equiv) and the reaction mixture was stirred for 8 hours at reflux using a Dean-Stark trap). Then, the mixture was cooled to room temperature and quenched with 1N NaOH and extracted with ethyl acetate, washed with brine, dried over magnesium sulfate, and concentrated in vacuo. The crude mixture was used for the next step without further purification.

[0807] To a solution of 3-(5-bromo-2-iodo-anilino)-2H-pyran-5-one (1300 mg, 3.3 mmol, 1 equiv) in DMSO (13.2 mL) was added L-proline (76 mg, 0.66 mmol, 0.2 equiv), KOH (740 mg, 13.2 mmol, 4 equiv), and CuI (63 mg, 0.33 mmol, 0.1 equiv), and the reaction mixture was stirred at 90 C. for 14 hours. The reaction mixture was cooled to room temperature and water/ethyl acetate was added, 1 N aqueous HCl was slowly added and the organic phase was combined, washed with brine, and dried over magnesium sulfate. The crude mixture was purified by silica gel chromatography (ethyl acetate/hexanes) to give 7-bromo-1,9-dihydropyrano[3,4-b]indol-4-one (640 mg, 73% yield over 2 steps). ESI-MS m/z=266.0 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 12.09 (s, 1H), 7.82 (d, J=8.3 Hz, 1H), 7.70 (d, J=1.8 Hz, 1H), 7.34 (dd, J=8.3, 1.8 Hz, 1H), 4.99 (s, 2H), 4.15 (s, 2H).

[0808] Step B

[0809] To a solution of 7-bromo-1,9-dihydropyrano[3,4-b]indol-4-one (600 mg, 2.25 mmol, 1 equiv) and [(2R)-3-bromo-2-methyl-propoxy]-tert-butyl-diphenyl-silane (1.324 g, 3.38 mmol, 1.5 equiv) in DMF (35 mL) was added potassium carbonate (935 mg, 6.76 mmol, 3 equiv) and NaI (34 mg, 0.23 mmol, 0.1 equiv) at room temperature and the reaction mixture was stirred for 16 hours at 70 C. The reaction was cooled and quenched with water and diluted with ethyl acetate. The separated organic layers were washed with brine. The organics were separated and dried over magnesium sulfate before concentration to dryness. The crude product was then purified by flash column chromatography eluting with ethyl acetate and hexanes to give 7-bromo-9-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-1H-pyrano[3,4-b]indol-4-one (900 mg, 69% yield). ESI-MS m/z=575.7 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3)O 8.05 (d, J=8.4 Hz, 1H), 7.66-7.62 (m, 4H), 7.55 (d, J=1.7 Hz, 1H), 7.49-7.36 (m, 7H), 4.92 (s, 2H), 4.31 (dd, J=14.5, 6.2 Hz, 1H), 4.27-4.16 (m, 2H), 3.76 (dd, J=14.5, 8.5 Hz, 1H), 3.61 (dd, J=10.5, 4.0 Hz, 1H), 3.51 (dd, J=10.6, 6.5 Hz, 1H), 2.29-2.12 (m, 1H), 1.13 (s, 9H), 0.87 (d, J=6.8 Hz, 3H).

[0810] Step C

[0811] To a solution of 7-bromo-9-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-1H-pyrano[3,4-b]indol-4-one (305 mg, 0.53 mmol, 1 equiv) in THF (7.5 mL) was added MeMgBr (3 M in ether, 0.44 mL, 1.32 mmol, 2.5 equiv) at 0 C., and the reaction mixture was stirred for 1 hours at 0 C. The reaction was quenched with ammonium chloride (aqueous) and diluted with ethyl acetate. Separated organic layers were washed with brine. The organics were then dried over magnesium sulfate before concentration to dryness. The crude mixture was quickly used for the next step without further purification.

[0812] Step D

[0813] To a solution of 7-bromo-9-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-4-methyl-1,3-dihydropyrano[3,4-b]indol-4-ol (300 mg, 0.51 mmol, 1 equiv) in dichloromethane (8.4 mL) was added TMSCN (0.25 mL, 2.02 mmol, 4 equiv) and BF.sub.3.OEt.sub.2 (0.16 mL, 1.27 mmol, 2.5 equiv) sequentially at 78 C., and the reaction mixture was stirred for 90 minutes. The reaction was quenched with aqueous sodium bicarbonate at 78 C., and diluted with dichloromethane. Separated organic layers were washed with saturated brine solution. The organics were then separated and dried (magnesium sulfate) before concentration to dryness. The crude was then purified by flash column chromatography eluting with ethyl acetate and hexanes to give 7-bromo-9-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-4-methyl-1,3-dihydropyrano[3,4-b]indole-4-carbonitrile (284 mg, 93% over 2 steps). .sup.1H NMR (400 MHz, CDCl.sub.3) 7.64 (m, 8H), 7.58 (d, J=8.5 Hz, 2), 7.54-7.49 (m, 2H), 7.48-7.36 (m, 12H), 7.28 (dd, J=8.6, 1.4 Hz, 2H), 4.84-4.70 (m, 2H), 4.19 (dd, J=6.0, 2.8 Hz, 1H), 4.15 (dd, J=6.4, 3.0 Hz, 1H), 4.10 (d, J=11.0 Hz, 1H), 4.04 (d, J=11.1 Hz, 1H), 3.83 (d, J=11.1 Hz, 1H), 3.79 (d, J=11.1 Hz, 1H), 3.69-3.61 (m, 2H), 3.60-3.54 (m, 2H), 3.53-3.43 (m, 2H), 2.19-2.08 (m, 2H), 1.75 (s, 3H), 1.73 (s, 3H), 0.86 (d, J=3.7 Hz. 3H), 0.84 (d, J=3.7 Hz. 3H).

[0814] Step D

[0815] To a solution of 7-bromo-9-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-4-methyl-1,3-dihydropyrano[3,4-b]indole-4-carbonitrile (284 mg, 0.47 mmol, 1 equiv) in THF (7.8 mL) was added TBAF (1 M in THF, 0.57 mL, 0.57 mmol, 1.2 equiv) at room temperature and the reaction mixture was stirred for 1 hour at room temperature. The reaction was quenched with aqueous ammonium chloride and diluted with ethyl acetate. Separated organic layers were washed with saturated brine solution. The organics were then separated and dried over magnesium sulfate before concentration to dryness. The crude was then purified by flash column chromatography eluting with ethyl acetate and hexanes. The desired fractions were concentrated to dryness in vacuo to give 7-bromo-9-[(2S)-3-hydroxy-2-methyl-propyl]-4-methyl-1,3-dihydropyrano[3,4-b]indole-4-carbonitrile (167 mg, 97%). ESI-MS m/z=363.0 [M+H].sup.+. .sup.1H NMR (500 MHz, CDCl.sub.3) 7.61 (d, J=8.3 Hz, 2H), 7.54 (d, J=1.7 Hz, 1H), 7.53 (d, J=1.6 Hz, 1H), 7.30 (dd, J=8.4, 1.7 Hz, 2H), 4.98-4.88 (m, 2H), 4.88-4.77 (m, 2H), 4.20-4.11 (m, 2H), 4.12-4.02 (m, 2H), 3.93-3.85 (m, 2H), 3.79-3.68 (m, 2H), 3.57-3.40 (m, 2H), 2.31-2.15 (m, 2H), 1.78 (s, 3H), 1.78 (s, 3H), 1.02 (d, J=3.6 Hz, 3H), 1.00 (d, J=3.5 Hz, 3H).

BM: 6-bromo-4-((S)-3-hydroxy-2-methylpropyl)-1,2,3,4-tetrahydrocyclopenta[b]indole-1-Carbonitrile

[0816] ##STR00309##

[0817] Step A

[0818] A mixture of 5-bromo-2-iodoaniline (5.0 g, 16.8 mmol, 1.0 equiv) and cyclopentane-1,3-dione (1.65 g, 16.8 mmol, 1.0 equiv) in toluene (50 mL) was stirred at 120 C. for 16 hours. The mixture was concentrated, and the residue was purified by silica gel chromatography (dichloromethane/MeOH=40/1) to give 3-((5-bromo-2-iodophenyl)amino)cyclopent-2-en-1-one (4.3 g, 68% yield) as a yellow solid. ESI-MS m/z=377.9 [M+H].sup.+.

[0819] Step B

[0820] A mixture of 3-((5-bromo-2-iodophenyl)amino)cyclopent-2-en-1-one (1.0 g, 2.56 mmol, 1.0 equiv), CuI (100 mg, 0.529 mmol, 0.2 equiv), KOH (594 mg, 10.6 mmol, 4.0 equiv), and L-proline (122 mg, 1.06 mmol, 0.4 equiv) in DMSO (100 mL) was stirred at 90 C. for 18 hours. The mixture was diluted with ethyl acetate (1.5 L) and washed with water (500 mL2) and brine (500 mL). The organic phase was dried over sodium sulfate, filtered and concentrated to give a residue that was used to next step without further purification. ESI-MS m/z=250.1 [M+H].sup.+

[0821] Step C

[0822] A mixture of 6-bromo-3,4-dihydrocyclopenta[b]indol-1(2H)-one (500 mg, 2 mmol, 1.0 equiv), K.sub.2CO.sub.3 (828 mg, 6 mmol, 3.0 equiv), KI (332 mg, 2 mmol, 1.0 equiv), and (R)-(3-bromo-2-methylpropoxy)(tert-butyl)diphenylsilane (782 mg, 2 mmol, 1.0 equiv) in DMSO (7 mL) was stirred at 110 C. for 16 hours. The mixture was diluted with ethyl acetate (50 mL), then washed with water (50 mL2) and brine (50 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was purified by silica gel chromatography (ethyl acetate/Petroleum ether=1/30) to give (S)-6-bromo-4-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3,4-dihydrocyclopenta[b]indol-1(2H)-one (700 mg, 63% yield) as a yellow oil. ESI-MS m/z=560.2 [M+H].sup.+.

[0823] Step D

[0824] LAH (1 M in THF, 3.1 mL, 3.0 equiv) was added to a solution of (S)-6-bromo-4-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3,4-dihydrocyclopenta[b]indol-1(2H)-one (700 mg, 1.25 mmol, 1.0 equiv) in THF (10 mL) at 0 C. The resulting solution was stirred for 0.5 hours at 0-5 C. Sodium sulfate.10H.sub.2O was added to the reaction mixture, and the mixture was diluted with ethyl acetate (50 mL) and filtered through celite. The filtrate was concentrated to give a crude residue that was used without further purification. ESI-MS m/z=544.1 [MH.sub.2O+H].sup.+.

[0825] Step E

[0826] A solution of TMSCN (123 mg, 1.24 mmol, 2.0 equiv) in dichloromethane (8 mL) was added to InBr.sub.3 (22 mg, 0.062 mmol, 0.1 equiv) under N2 and the mixture was stirred at 15 C. for 0.5 hours. A solution of 6-bromo-4-((S)-3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1,2,3,4-tetrahydrocyclopenta[b]indol-1-ol (350 mg, 0.62 mmol, 1.0 equiv) in dichloromethane (2 mL) was then added at 0 C. to 5 C., and the final mixture was stirred at 15 C. for 1 hour. Saturated aqueous NaHCO.sub.3(20 mL) was added to the reaction mixture and the organic phase was collected and washed with water (20 mL2) and brine (20 mL). The organics were dried over sodium sulfate, filtered and concentrated to give a residue that was purified by silica gel chromatography (ethyl acetate/Petroleum ether (1:10)) to give 6-bromo-4-((S)-3-((tert-butyldiphenylsilyloxy)-2-methylpropyl)-1,2,3,4-tetrahydrocyclopenta[b]indole-1-carbonitrile (200 mg, 28% yield) as a light yellow solid. ESI-MS m/z=593.2 [M+Na].sup.+.

[0827] Step F

[0828] TBAF (1 M in THF, 0.88 mL, 2.0 equiv) was added to a solution of 6-bromo-4-((S)-3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1,2,3,4-tetrahydrocyclopenta[b]indole-1-carbonitrile (250 mg, 0.44 mmol, 1.0 equiv) in THF (5 mL). The resulting solution was stirred for 1 hour at 20 C. The mixture was diluted with ethyl acetate (20 mL) and washed with water (20 mL6) and brine (20 mL). The organic layer was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was purified by silica gel chromatography (ethyl acetate/Petroleum ether (1:1)) to give 6-bromo-4-((S)-3-hydroxy-2-methylpropyl)-1,2,3,4-tetrahydrocyclopenta[b]indole-1-carbonitrile (100 mg, 94% yield) as an off-white solid. ESI-MS m/z=333.1 [M+H].sup.+.

[0829] The following intermediate was synthesized according to the procedure described to make Intermediate BM using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00020 Intermediate No. Structure Analytical Data BM-1 [00310] ES-MS m/z = 374.0 [M + H]+. .sup.1H NMR (400 MHz, DMSO- d.sub.6) 7.74 (s, 1H), 7.55-7.42 (m, 2H), 7.38-7.29 (m, 1H), 7.16 (d, J = 8.3 Hz, 2H), 7.07 (t, J = 7.4 Hz, 1H), 6.39 (s, 1H). 4.50 (t, J = 5.1 Hz, 1H), 4.11 (dd, J = 14.5, 6.1 Hz, 1H), 3.76 (s, 3H), 3.74-3.66 (m, 1H), 3.05 (t, J = 5.4 Hz, 2H), 1.79 (dd, J = 13.3, 6.7 Hz, 1H), 0.45 (d, J = 6.7 Hz, 3H).

BN: 3-(6-bromo-3-(3-methoxy-2-methylbutan-2-yl)-1H-Indol-1-yl)-2,2-dimethylpropan-1-ol

[0830] ##STR00311##

[0831] Step A

[0832] To a stirred solution of 3-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)-3-methylbutan-2-one (900 mg, 1.87 mmol, 1.0 equiv) in MeOH (10 mL) at 0 C. was added NaBH.sub.4 (354 mg, 9.36 mmol, 5.0 equiv) in portions. The resulting mixture was stirred for 4 hours at room temperature. The resulting mixture was diluted with water (200 mL) and extracted with ethyl acetate (2200 mL). The combined organic layers were washed with water (2100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluting with ethyl acetate/petroleum ether (1:12 to 1:7) to afford 3-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)-3-methylbutan-2-ol (900 mg, 90% yield) as a yellow oil. ESI-MS m/z=483.2 [M+H].sup.+.

[0833] Step B

[0834] To a stirred solution of 3-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)-3-methylbutan-2-ol (900 mg, 1.483 mmol, 1.0 equiv) in dichloromethane (10 mL) was added tetrafluoroboric acid (240 mg, 1.48 mmol, 1.0 equiv) and TMSCHN.sub.2 (9 mL) dropwise at 0 C. The resulting mixture was stirred for 5 minutes at 0 C., and then diluted with water (200 mL). The resulting mixture was extracted with ethyl acetate (2200 mL). The combined organic layers were washed with water (2100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-3-(3-methoxy-2-methylbutan-2-yl)-1H-indole (600 mg, crude) was used in the next step directly without further purification. ESI-MS m/z=496.3 [M+H].sup.+.

[0835] Step C

[0836] To a stirred solution of 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-3-(3-methoxy-2-methylbutan-2-yl)-1H-indole (600 mg, 0.705 mmol, 1.0 equiv) in THF (7 mL) at 0 C. was added TBAF (1N in THF, 1.5 mL) dropwise. The resulting mixture was stirred for 1 hour at 0 C. After removal of solvent, the residue was purified by prep-TLC (petroleum ether/ethyl acetate (2:1)) to afford 3-(6-bromo-3-(3-methoxy-2-methylbutan-2-yl)-1H-indol-1-yl)-2,2-dimethylpropan-1-ol (350 mg, 68% yield) as a yellow oil. ESI-MS m/z=382.1 [M+H].sup.+.

BO: 2-(6-bromo-1-((S)-3-hydroxy-2-methylpropyl)-1H-Indol-3-yl-2-cyclopropylacetonitrile

[0837] ##STR00312##

[0838] Step A

[0839] To a stirred solution of [(2R)-3-bromo-2-methylpropoxy](tert-butyl)diphenylsilane (4.67 g, 11.9 mmol, 1.2 equiv) and Cs.sub.2CO.sub.3 (4.86 g, 14.9 mmol, 1.5 equiv) in DMF (50 mL) was added 6-bromo-1H-indole-3-carbaldehyde (2.23 g, 9.9 mmol, 1.0 equiv) dropwise. The mixture was stirred for 3 days at room temperature. Water (500 mL) and ethyl acetate (300 mL) were added. The combined organic layers were washed with brine (3200 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (5:1) to provide (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indole-3-carbaldehyde (4.8 g, 90% yield) as a yellow oil. ESI-MS m/z=534.1 [M+H].sup.+.

[0840] Step B

[0841] To a stirred solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indole-3-carbaldehyde (2.13 g, 3.99 mmol, 1.0 equiv) in THF (30 mL) was added cyclopropylmagnesiumbromide (1 M, 9.2 mL) dropwise at 0 C. The resulting mixture was stirred for 2 hours at 0 C. The reaction was quenched by the addition of saturated aqueous NH.sub.4Cl (30 mL) at 0 C. Ethyl acetate (30 mL) was added and the organic layer was washed with brine (3100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the crude product was used in the next step directly without further purification. ESI-MS m/z=598.2 [M+Na].sup.+.

[0842] Step C

[0843] To a stirred solution (6-bromo-1-((S)-3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indol-3-yl)(cyclopropyl)methanol (2.3 g, 3.989 mmol, 1.0 equiv) in dichloromethane (20 mL) at 78 C. was added TMSCN (1980 mg, 19.9 mmol, 5.0 equiv) and BF.sub.3.Et.sub.2O (1.415 g, 9.97 mmol, 2.5 equiv) dropwise. The resulting mixture was stirred for 1.5 hours at 78 C. The reaction was quenched by the addition of saturated aqueous Na.sub.2CO.sub.3 (30 mL) and then diluted with dichloromethane (30 mL). The organic layer was washed with brine (3100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (5:1) to afford 2-(6-bromo-1-((S)-3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indol-3-yl)-2-cyclopropylacetonitrile (1.5 g, 64% yield) as a yellow oil. .sup.1H NMR (300 MHz, DMSO-d.sub.6) 7.76 (s, 1H), 7.58 (t, J=7.0 Hz, 5H), 7.47-7.39 (m, 7H), 7.24 (d, J=8.6 Hz, 1H), 4.25 (t, J=10.5 Hz, 1H), 4.08 (t, J=8.8 Hz, 2H), 3.49 (d, J=5.4 Hz, 2H), 2.17 (d, J=6.4 Hz, 1H), 1.37 (s, 1H), 1.04 (s, 9H), 0.87 (d, J=6.7 Hz, 3H), 0.63 (s, 1H), 0.54 (s, 1H), 0.47-0.30 (m, 2H).

[0844] Step D

[0845] To a stirred solution of 2-(6-bromo-1-((S)-3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indol-3-yl)-2-cyclopropylacetonitrile (1.0 g, 1.707 mmol, 1 equiv) in THF (10 mL) was added HF-pyridine (1 mL, 40%) dropwise at 0 C. The resulting mixture was stirred for 16 hours and then the reaction was basified to pH 8 with saturated aqueous NaHCO.sub.3. The mixture was extracted with ethyl acetate (330 mL) and the combined organic layers were washed with brine (310 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the residue was purified by Prep-TLC (petroleum ether/ethyl acetate (2:1)) to afford 2-(6-bromo-1-((S)-3-hydroxy-2-methylpropyl)-1H-indol-3-yl)-2-cyclopropylacetonitrile (580 mg, 99% yield) as a yellow oil. .sup.1H NMR (300 MHz, DMSO-d.sub.6) =7.78 (d, J=1.7 Hz, 1H), 7.62 (d, J=8.5 Hz, 1H), 7.45 (s, 1H), 7.23 (dd, J=8.5, 1.7 Hz, 1H), 4.75-4.60 (m, 1H), 4.20 (dd, J=14.5, 6.3 Hz, 1H), 4.10 (d, J=8.1 Hz, 1H), 3.99 (dd, J=13.8, 7.1 Hz, 1H), 3.25 (dq, J=10.7, 5.5 Hz, 2H), 2.05 (dt, J=13.2, 6.4 Hz, 1H), 1.51-1.39 (m, 1H), 0.81 (d, J=6.7 Hz, 3H), 0.71-0.56 (m, 2H), 0.43 (ddt, J=22.4, 9.5, 4.7 Hz, 2H).

BP: 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indole-3-carbonitrile

[0846] ##STR00313##

[0847] Step A

[0848] A solution of 6-bromo-1H-indole-3-carboxamide (2.39 g, 10.0 mmol, 1.0 equiv), K.sub.2CO.sub.3 (2.76 g, 20.0 mmol, 2.0 equiv), KI (1.66 g, 10.0 mmol, 1.0 equiv), and (3-bromo-2,2-dimethylpropoxy)(tert-butyl)dimethylsilane (4.22 g, 15.0 mmol, 1.5 equiv) in DMSO (15 mL) was stirred at 150 C. overnight.

[0849] After cooling to room temperature, ice water (100 mL) was added and the solution was extracted with ethyl acetate (3100 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give a crude product, which was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH (20:1)) to give 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indole-3-carboxamide (3.25 g, 74% yield) as an oil. ESI-MS m/r=439.1 [M+H].sup.+.

[0850] Step B

[0851] To a solution of 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indole-3-carboxamide (3.0 g, 6.83 mmol, 1.0 equiv) in pyridine (30 mL) at 0 C. was added POCl.sub.3 (5.23 g, 34.2 mmol) dropwise. The mixture was stirred at 0 C. for 2 hours. The mixture was poured into water and extracted with ethyl acetate (200 mL). The organic layer was washed with brine (50 mL3), dried over anhydrous sodium sulfate, filtered and concentrated to give a residue. The residue was purified by silica gel (petroleum ether to petroleum ether/ethyl acetate (5:1)) to give 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indole-3-carbonitrile (2.18 g, 76% yield) as a white solid.

[0852] Step C

[0853] To a solution of 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indole-3-carbonitrile (2.11 g, 5.0 mmol, 1.0 equiv) in THF (20 mL) at 0 C. was added TBAF (7.5 mL, 1 M in THF) dropwise. The mixture was stirred at 0 C. for 2 hours. The mixture was poured into water and extracted with ethyl acetate (200 mL). The organic phase was washed with brine (50 mL3) and dried over anhydrous sodium sulfate, filtered, and concentrated to give a residue. The residue was purified by silica gel (petroleum ether to petroleum ether/ethyl acetate (3:1)) to give 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indole-3-carbonitrile (1.25 g, 81% yield) as a white solid. ESI-MS m/z=309.0 [M+H].sup.+.

BQ: (S)-6-bromo-1-(3-hydroxy-2-methylpropyl)-2-(2-methoxyphenyl)-1H-indole-3-carbonitrile

[0854] ##STR00314##

[0855] Step A

[0856] A solution of 6-bromo-1H-indole-3-carbonitrile (2.0 g, 9.1 mmol, 1.0 equiv), (R)-(3-bromo-2-methylpropoxy)(tert-butyl)diphenylsilane (5.3 g, 13.6 mmol, 1.5 equiv). KI (1.5 g, 9.1 mmol, 1.0 equiv), K.sub.2CO.sub.3 (3.8 g, 27.3 mmol, 3.0 equiv), and DMSO (80 mL) was stirred at 130 C. for 16 hours. H.sub.2O (100 mL) was added and the resulting solution was extracted with ethyl acetate (200 mL3). The combined organic layers were concentrated under reduced pressure to give a residue. The residue was purified by silica gel chromatography (petroleum ether to petroleum ether/ethyl acetate (95:5)) to afford (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl) oxy)-2-methylpropyl)-1H-indole-3-carbonitrile (4.6 g, 90% yield) as a white solid. ESI-MS m/z=553.2 [M+Na].sup.+.

[0857] Step B

[0858] To a solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indole-3-carbonitrile (1.0 g, 2.20 mmol, 1.0 equiv) and boron isopropoxide (752 mg, 4.0 mmol, 1.8 equiv) in THF (10 mL) was added LDA (2 M in THF/hexanes, 2.2 mL, 4.4 mmol, 2.0 equiv) dropwise at 78 C. The mixture was stirred at 78 C. for 0.5 hours, 10 mL ice water was added to the mixture. After warming to room temperature, the layers were separated, and the aqueous phase was extracted with ethyl acetate (20 mL3). The combined organic layers were concentrated under reduced pressure to afford 6-bromo-1-(tert-butoxycarbonyl)-1H-indol-2-yl)boronic acid as a colorless gum. The residue was used for next step without further purification. ESI-MS m/z=596.8 [M+Na].sup.+.

[0859] Step C

[0860] A solution of (6-bromo-1-(tert-butoxycarbonyl)-1H-indol-2-yl)boronic acid (2.14 g, 3.70 mmol, 1.0 equiv), 1-iodo-2-methoxybenzene (1.30 g, 5.6 mmol, 1.5 equiv), Pd(dppf)Cl.sub.2 (410 mg, 0.1 equiv), and K.sub.2CO.sub.3 (1.5 g, 11.1 mmol, 3.0 equiv) in toluene (20 mL) and H.sub.2O (12 mL) was stirred at 60 C. for 2 hours. The mixture was separated, and the aqueous phase was extracted with ethyl acetate (20 mL3). The combined organic layers were dried and concentrated under reduced pressure to give a residue. The residue was purified by silica gel chromatography (Petroleum/ethyl acetate (3:1 to 1:1)) to afford (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl) oxy)-2-methylpropyl)-2-(2-methoxyphenyl)-1H-indole-3-carbonitrile (812 mg, 19% yield, 2 steps) as a light-yellow gum. ESI-MS m/z=658.9 [M+Na].sup.+.

[0861] Step D

[0862] To a solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-2-(2-methoxyphenyl)-1H-indole-3-carbonitrile (1.5 g, 2.36 mmol, 1.0 equiv) in THF (10 mL) was added TBAF (3.5 mL, 1.0 M in THF, 3.5 mmol, 1.5 equiv). The mixture was stirred at 20 C. for 1 hour and then 250 mL of ethyl acetate was poured into the mixture. The resulting solution was then washed with water (10 mL8). The organic layer was concentrated under reduced pressure to afford (S)-6-bromo-1-(3-hydroxy-2-methylpropyl)-2-(2-methoxyphenyl)-1H-indole-3-carbonitrile (1.4 g) as a colorless gum. The crude product was carried on without further purification. ESI-MS m/z=398.9 [M+H].sup.+.

[0863] The following intermediate was synthesized according to the procedure described to make Intermediate BQ using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00021 Intermediate No. Structure Analytical Data BQ-1 [00315] ESI-MS m/z = 374.0 [M + H]+. .sup.1H NMR (400 MHz, DMSO- d.sub.6) 7.74 (s, 1H), 7.55-7.42 (m, 2H), 7.38-7.29 (m, 1H), 7.16 (d, J = 8.3 Hz, 2H), 7.07 (t, J = 7.4 Hz, 1H), 6.39 (s, 1H), 4.50 (t, J = 5.1 Hz, 1H), 4.11 (dd, J = 14.5, 6.1 Hz, 1H), 3.76 (s, 3H), 3.74-3.66 (m, 1H), 3.05 (t, J = 5.4 Hz, 2H), 1.79 (dd, J = 13.3, 6.7 Hz, 1H), 0.45 (d, J = 6.7 Hz, 3H).

BR: (S)-3-(6-bromo-3-ethyl-2-(2-methoxyphenyl)-1H-indol-1-yl)-2-methylpropan-1-ol

[0864] ##STR00316##

[0865] Step A

[0866] A mixture of (S)-3-(6-bromo-2-(2-methoxyphenyl)-1H-indol-1-yl)-2-methylpropan-1-ol (1.1 g, 2.9 mmol, 1.0 equiv) and NIS (980 mg, 4.3 mmol, 1.5 equiv) in CH.sub.3CN (50 mL) was stirred for 4 hours at 20 C. The reaction mixture was concentrated in vacuo. The residue was dissolved in ethyl acetate (30 mL) and washed with water (20 mL3). The organic layer was dried, filtered, and the solvent removed under reduced pressure. The residue was purified by silica gel chromatography (ethyl acetate/Petroleum ether, 1/3) to yield (S)-3-(6-bromo-3-iodo-2-(2-methoxyphenyl)-1H-indol-1-yl)-2-methylpropan-1-ol (1.3 g) as a white solid.

[0867] Step B

[0868] To a mixture of (S)-3-(6-bromo-3-iodo-2-(2-methoxyphenyl)-1H-indol-1-yl)-2-methylpropan-1-ol (850 mg, 1.7 mmol, 1.0 equiv), K.sub.2CO.sub.3 (705 mg, 5.1 mmol, 3.0 equiv) and potassium vinyltrifluoroborate (455 mg, 3.4 mmol, 2.0 equiv) in toluene/H.sub.2O (5/1, 20 mL) was added Pd(dppf)Cl.sub.2*CH.sub.2Cl.sub.2 (140 mg, 0.1 equiv). After stirring for 3 hours at 110 C., the reaction mixture was cooled to room temperature and filtered. The filtrate was diluted with ethyl acetate, washed with brine, and concentrated in vacuo to yield (S)-3-(6-bromo-2-(2-methoxyphenyl)-3-vinyl-1H-indol-1-yl)-2-methylpropan-1-ol (900 mg, crude) as a dark brown solid. The residue was used for the next step without further purification. ESI-MS m/z=400.1 [M+H].sup.+.

[0869] Step C

[0870] A mixture of (S)-3-(6-bromo-2-(2-methoxyphenyl)-3-vinyl-1H-indol-1-yl)-2-methylpropan-1-ol (900 mg, crude), diludine (860 mg, 3.4 mmol), and TsOH.H.sub.2O (30 mg) in dichloromethane (30 mL) was stirred for 4 hours at 20 C. The reaction mixture was diluted with dichloromethane, washed with brine, dried over sodium sulfate, filtered, and concentrated in vacuo. The residue was purified by silica gel chromatography (40% ethyl acetate in Petroleum ether) to give (S)-3-(6-bromo-3-ethyl-2-(2-methoxyphenyl)-1H-indol-1-yl)-2-methylpropan-1-ol (410 mg, 60% yield, 2 steps) as a light yellow solid. ESI-MS m/z=402.1 [M+H].sup.+.

BS: (S)-6-bromo-3-(2-cyanopropan-2-yl)-1-(3-hydroxy-2-methylpropyl)-1H-indole-2-carbonitrile

[0871] ##STR00317##

[0872] Step A

[0873] To a stirred solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indole-3-carbaldehyde (1.06 g, 2.0 mmol, 1.0 equiv) in DMSO (dry, 30 mL) was added diethyl phosphoryl cyanide (2.0 g, 11.9 mmol, 6.0 equiv) followed by NaCN (0.6 g, 11.9 mmol, 6.0 equiv) at 0 C. The mixture was stirred at 0 C. to 10 C. for 1 hour. The solution was poured into ice water (200 mL) and extracted with ethyl acetate (100 mL3). The combined organic layers were dried and then concentrated to dryness to give a residue. The residue was purified by reverse phase chromatography (90% CH.sub.3CN/water) to give (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-(cyanomethyl)-1H-indole-2-carbonitrile (810 mg, 75% yield) as an oil. ESI-MS m/z=592.2 [M+Na].sup.+.

[0874] Step B

[0875] (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-(cyanomethyl)-1H-indole-2-carbonitrile (2.0 g, 3.5 mmol, 1.0 equiv) in THF (50 mL) at 78 C. was treated with NaHMDS (2M in THF, 5.25 mL, 10.5 mmol, 3.0 equiv). The mixture was stirred at 78 C. for 1 hour and then MeI (1.5 g, 10.5 mmol, 3.0 equiv) was added. The reaction mixture was stirred at 78 C. for 30 minutes. The solution was poured into water (1 L) and the solution was extracted with ethyl acetate (300 mL3). The combined organic layers were washed with brine (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate (20:1)) to give (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-(2-cyanopropan-2-yl)-1H-indole-2-carbonitrile (2.4 g, 57% yield) as an oil. ESI-MS m/z=620.1 [M+Na].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.96 (d, J=8.8 Hz, 1H), 7.66 (ddd, J=6.4, 3.4, 1.8 Hz, 5H), 7.47-7.35 (m, 7H), 4.51 (dd, J=14.7, 5.7 Hz, 1H), 4.06 (dd, J=14.7, 8.7 Hz, 1H), 3.63 (dd, J=10.5, 4.4 Hz, 1H), 3.52 (dd, J=10.5, 6.9 Hz, 1H), 2.33-2.23 (m, 1H), 1.98 (d, J=8.1 Hz, 6H), 1.11 (d, J=11.4 Hz, 9H), 0.86 (d, J=6.8 Hz, 3H).

[0876] Step C

[0877] To a stirred solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-(2-cyanopropan-2-yl)-1H-indole-2-carbonitrile (1.6 g, 2.67 mmol, 1.0 equiv) in THF (25 mL) was added AcOH (321 mg, 5.34 mmol, 2.0 equiv) and TBAF (1 M in THF, 5.34 mL, 5.34 mmol, 2.0 equiv) at 0 C. to 5 C. The resulting mixture was stirred for 6 hours at 0 C. to 5 C. After concentration, the crude product was diluted with ethyl acetate (50 mL) and washed with water (10 mL5). The organic layer was concentrated under reduced pressure to give a residue. The residue was purified by silica gel chromatography (Petroleum ether/ethyl acetate (3:1)) to give (S)-6-bromo-3-(2-cyanopropan-2-yl)-1-(3-hydroxy-2-methylpropyl)-1H-indole-2-carbonitrile (870 mg, 90% yield) as a white solid. ESI-MS m/z=360.1 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 8.03 (d, J=1.6 Hz, 1H), 7.93 (d, J=8.8 Hz, 1H), 7.43 (dd, J=8.8, 1.7 Hz, 1H), 4.76 (s, 1H), 4.38 (dd, J=14.8, 6.5 Hz, 1H), 4.11 (dd, J=14.8, 8.3 Hz, 1H), 3.32 (s, 2H), 2.12 (dd, J=13.7, 6.5 Hz, 1H), 1.94 (s, 6H), 0.82 (d, J=6.8 Hz, 3H).

BT 2-(6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indol-3-yl)-2-cyclopentylpropanenitrile

[0878] ##STR00318##

[0879] Step A

[0880] SnCl.sub.4 (1 M in dichloromethane, 30.6 mL, 1.2 equiv) was added to a solution of 6-bromo-1H-indole (5.0 g, 25.5 mmol, 1.0 equiv) in dichloromethane (50 mL) at 0 C. The mixture was stirred at 15 C. for 30 minutes. A solution of cyclopentanecarboxylic acid (2.91 g, 25.5 mmol, 1.0 equiv) in SOCl.sub.2 (0.5 mL) was stirred at 90 C. for 1 hour. The mixture was concentrated to give a residue that was dissolved in dichloromethane (20 mL) and then added to the above mixture, then the final mixture was stirred at 15 C. for 30 min. The reaction was quenched by addition of saturated NaHCO.sub.3 (20 mL) and the solution was then diluted with ethyl acetate (20 mL). The organic phase was separated and washed with water (20 mL2) and brine (20 mL) and dried over sodium sulfate. After filtration and concentration, the crude residue was purified by silica gel chromatography (ethyl acetate/Petroleum ether (1:10)) to give (6-bromo-1H-indol-3-yl)(cyclopentyl)methanone (1.98 g, 27% yield) as a brown solid. ESI-MS m/z=292.0 [M+H].sup.+.

[0881] Step B

[0882] A mixture of (6-bromo-1H-indol-3-yl)(cyclopentyl)methanone (2.0 g, 6.85 mmol, 1.0 equiv), Cs.sub.2CO.sub.3 (6.7 g, 20.6 mmol, 3.0 equiv), and (3-bromo-2,2-dimethylpropoxy)(tert-butyl)dimethylsilane (3.85 g, 13.7 mmol, 2.0 equiv) in DMSO (20 mL) was stirred at 100 C. for 16 hours. The mixture was diluted with ethyl acetate (100 mL) and washed with water (100 mL2) and brine (100 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was concentrated and purified by silica gel chromatography (ethyl acetate/Petroleum ether (1:20)) to give (6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)(cyclopentyl)methanone (1.4 g, 42% yield) as a light yellow solid. ESI-MS m/z=492.2 [M+H].sup.+.

[0883] Step C

[0884] MeMgBr (3M in THF, 1.53 mL, 2.5 equiv) was added to a solution of (6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)(cyclopentyl)methanone (900 mg, 1.83 mmol, 1.0 equiv) in THF (20 mL) at 0 C. The solution was stirred at 0 C. to 5 C. for 1 hour. The reaction was quenched carefully by addition of saturated NH.sub.4Cl (2 mL) at 0 C. The mixture was diluted with ethyl acetate (25 mL), then washed with brine (15 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was used in the next step without further purification. ESI-MS m/z=490.2 [MH.sub.2O+H].sup.+.

[0885] Step D

[0886] A solution of TMSCN (363 mg, 3.66 mmol, 2.0 equiv) in dichloromethane (20 mL) was added to InBr.sub.3 (130 mg, 0.2 mmol, 0.2 equiv) under N.sub.2 and the mixture was stirred at 15 C. for 30 minutes. Then a solution of 1-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)-1-cyclopentylethan-1-ol (930 mg, 1.83 mmol, 1.0 equiv) in dichloromethane (10 mL) was added at 0 C. to 5 C. and the mixture was stirred at 15 C. for 1 hour. Saturated aqueous NaHCO.sub.3 (20 mL) was added to the reaction mixture. The organic phase was collected, washed with water (50 mL2) and brine (50 mL), dried over sodium sulfate, filtered, and concentrated to give a residue. The residue was concentrated and purified by silica gel chromatography (ethyl acetate/Petroleum ether (1:30)) to give 2-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)-2-cyclopentylpropanenitrile (300 mg, 34% yield) as a light yellow solid. ESI-MS m/z=539.2 [M+Na].sup.+.

[0887] Step E

[0888] TBAF (1 M in THF, 2.32 mL, 2.0 equiv) was added to a solution of 2-(6-bromo-1-(3-((tert-butyidimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)-2-cyclopentylpropanenitrile (600 mg, 1.16 mmol, 1.0 equiv) in THF (20 mL). The resulting solution was stirred for 1 hour at 20 C. The mixture was diluted with ethyl acetate (20 mL) and then washed with water (20 mL3) and brine (20 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was purified by silica gel chromatography (ethyl acetate/Petroleum ether (3:1)) to give 2-(6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indol-3-yl)-2-cyclopentylpropanenitrile (380 mg, 97% yield) as an off-white solid. ESI-MS m/z=425.1 [M+Na].sup.+.

BU: 3-(6-bromo-3-ethyl-1H-indol-1-yl)-2,2-dimethylpropan-ol

[0889] ##STR00319##

[0890] Step A

[0891] To a solution of 6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indole-3-carbaldehyde (2 g, 4.72 mmol, 1.0 equiv) in THF (anhydrous, 15 mL) at 20 C. was added MeMgBr (9.5 mL, 9.5 mmol, 1 M in THF, 2.0 equiv) dropwise. After stirring for 2 hours at this same temperature, aqueous saturated NH.sub.4Cl (20 mL) was added and the resulting solution was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over sodium sulfate, filtered, and concentrated to give crude 1-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)ethan-1-ol (1.99 g, crude) which used in next step directly without further purification.

[0892] Step B

[0893] To a mixture of 1-(6-bromo-1-(3-((tert-butyldimethylsilyl)oxy)-2,2-dimethylpropyl)-1H-indol-3-yl)ethan-1-ol (1.99 g, crude, 1.0 equiv) and tosic acid hydrate (163 mg, 0.86 mmol, 0.2 equiv) in dichloromethane (20 mL) was added diethyl 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate (1.09 g, 4.30 mmol, 1.0 equiv). The resulting mixture was stirred for 3 hours at room temperature. The reaction mixture was diluted with dichloromethane and was washed with brine, dried over sodium sulfate, filtered, and concentrated to give the crude product. Purification by silica gel chromatography (ethyl aceate/petroleum ether (1:8)) afforded 6-bromo-1-(3-((tert-butyldimethylsilyl) oxy)-2,2-dimethylpropyl)-3-ethyl-1H-indole (1.22 g, 61% yield two steps).

[0894] Step C

[0895] 3-(6-Bromo-3-ethyl-1H-indol-1-yl)-2,2-dimethylpropan-1-ol was synthesized from 6-bromo-1-(3-((tert-butyldimethylsilyl) oxy)-2,2-dimethylpropyl)-3-ethyl-1H-indole using similar conditions to those described for the synthesis of 6-bromo-1-((1-(hydroxymethyl)cyclopropyl)methyl)-1H-indole-3-carbonitrile.

[0896] ESI-MS m/z=310.1 [M+H].sup.+.

BV: Synthesis of (S)-6-bromo-3-cyclopropyl-1-(3-hydroxy-2-methylpropy)-1H-indole-2-carbonitrile

[0897] ##STR00320##

[0898] Step A

[0899] To a stirred solution of 6-bromo-1H-indole-2-carbonitrile (3.5 g, 15.8 mmol, 1.0 equiv) in DMF (50 mL) at 0 C. was added NIS (3.8 g, 16.8 mmol, 1.05 equiv) in portions. The resulting mixture was stirred for 1 hour at 20 C., and then poured into water (200 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by silica gel chromatography (Petroleum ether/ethyl acetate (20:1)) to give 6-bromo-3-iodo-1H-indole-2-carbonitrile (5.1 g, 92% yield) as an oil.

[0900] Step B

[0901] To a stirred solution of 6-bromo-3-iodo-1H-indole-2-carbonitrile (5.1 g, 14.7 mmol, 1.0 equiv) in DMF (100 mL) was added (R)-(3-bromo-2-methylpropoxy)(tert-butyl)diphenylsilane (7.2 g, 22.0 mmol, 1.5 equiv) followed by K.sub.2CO.sub.3 (6.1 g, 44.1 mmol, 3.0 equiv). The mixture was stirred at 100 C. for 16 hours and then the solution was poured into water (800 mL) and extracted with ethyl acetate (250 mL3). The combined organic layers were washed with water (300 mL2) and brine (300 mL1) and dried over sodium sulfate and concentrated to give a residue. The residue was purified by silica gel chromatography (petroleum ether/ethyl acetate (100:1)) to give (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-iodo-1H-indole-2-carbonitrile (8.2 g, 84% yield) as an oil. ESI-MS m/z=679.0[M+Na].sup.+; .sup.1H NMR (400 MHz, CDCl.sub.3) 7.72-7.60 (m, 5H), 7.49-7.30 (m, 8H), 4.54 (dd, J=14.6, 5.8 Hz, 1H), 4.09 (dd, J=14.6, 8.6 Hz, 1H), 3.61 (dd, J=10.5, 4.3 Hz, 1H), 3.51 (dd, J=10.5, 7.0 Hz, 1H), 2.27 (dd, J=4.2, 2.6 Hz, 1H), 1.12 (s, 9H), 0.85 (t, J=6.0 Hz, 3H).

[0902] Step C

[0903] (S)-6-Bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-iodo-1H-indole-2-carbonitrile (2.0 g, 3.03 mmol, 1.0 equiv), potassium cyclopropyltrifluoroborate (540 mg, 3.64 mmol, 1.2 equiv), Pd(dppf)Cl.sub.2CH.sub.2Cl.sub.2 (400 mg, 0.49 mmol, 0.16 equiv) and K.sub.2CO.sub.3 (1.25 g, 9.05 mmol, 3.0 equiv) in toluene (80 mL) and water (15 mL) was stirred at 80 C. for 16 hours. After concentration, the residue was purified by C18 reverse phase chromatography (95% CH.sub.3CN/water) to afford (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-cyclopropyl-1H-indole-2-carbonitrile (1.8 g, 51% yield) after combining with a previous batch.

[0904] Step D

[0905] To a stirred solution of (S)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-3-cyclopropyl-1H-indole-2-carbonitrile (1.7 g, 3.0 mmol, 1.0 equiv) in THF (30 mL) was added TBAF (1 M in THF, 6.0 mL, 6.0 mmol, 2.0 equiv) at 0 C. to 5 C. The resulting mixture was stirred for 1 hour at 0 C. to 10 C. After concentration, the crude product was diluted with ethyl acetate (50 mL) and washed with water (10 mL5). The organic layer was dried and concentrated under reduced pressure to give a residue. The residue was purified by silica gel chromatography (Petroleum ether/ethyl acetate (10:1)) to give (S)-6-bromo-3-cyclopropyl-1-(3-hydroxy-2-methylpropyl)-1H-indole-2-carbonitrile (950 mg, 99% yield) as an oil. ESI-MS m/z=333.1 [M+H].sup.+. .sup.1H NMR (400 MHz. DMSO-d.sub.6) 7.89 (d, J=1.5 Hz, 1H), 7.64 (d, J=8.6 Hz, 1H), 7.28 (dd, J=8.6, 1.7 Hz, 1H), 4.73 (t, J=5.1 Hz, 1H), 4.28 (dd, J=14.7, 6.5 Hz, 1H), 4.01 (dd, J=14.7, 8.3 Hz, 1H), 3.28 (dd, J=9.2, 5.3 Hz, 2H), 2.09 (ddd, J=8.5, 5.3, 3.3 Hz, 2H), 1.10-1.02 (m, 2H), 0.92 (ddd, J=6.2, 5.2, 3.7 Hz, 2H), 0.78 (d, J=6.8 Hz, 3H).

BW: (R)-3-(6-bromo-2-(((3-(trimethylsilyl)prop-2-yn-1-yl)oxy)methy-1H-indol-1-yl)-2-methylpropan-1-ol

[0906] ##STR00321##

[0907] Step A

[0908] To a solution of 6-bromo-1H-indole-2-carbaldehyde (0.600 g, 2.25 mmol) and [(2R)-3-bromo-2-methyl-propoxy]-tert-butyl-diphenyl-silane (1.3 g, 3.38 mmol) in DMF (35 mL) was added K.sub.2CO.sub.3 (0.935 g, 6.76 mmol) and NaI (33.8 mg, 0.2300 mmol) and the reaction mixture was stirred for 16 hours at 70 C. The reaction was quenched with water and diluted with ethyl acetate. Separated organic layers was washed with saturated brine solution. The organics were then separated and dried (magnesium sulfate) before concentration to dryness. The crude product was then purified by flash column chromatography eluting with ethyl acetate and hexanes to give (R)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indole-2-carbaldehyde (0.900 g, 69% yield). .sup.1H NMR (400 MHz, CDCl.sub.3) 9.85 (s, 1H), 7.74-7.55 (m, 8H), 7.46-7.33 (m, 8H), 7.28 (dd, J=8.6, 1.6 Hz, 1H), 4.67-4.44 (m, 3H), 3.66-3.45 (m, 3H), 2.23 (ddd, J=8.0, 6.3, 4.6 Hz, 1H), 1.12 (s, 11H), 0.83 (d, J=6.8 Hz, 3H).

[0909] Step B

[0910] (R)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indole-2-carbaldehyde (0.650 g, 1.2 mmol) was dissolved in methanol (10 mL) and NaBH.sub.4 (0.092 g, 2.4 mmol, 2 equiv) was added in portions. The mixture was stirred for 1 hour at room temperature before being slowly quenched with water and extracted with ethyl acetate. Organics were dried over MgSO.sub.4, filtered and evaporated. Silica gel chromatography eluting with hexanes and ethyl acetate gave (R)-(6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indol-2-yl)methanol (0.556 g, 85% yield). .sup.1H NMR (400 MHz, CDCl.sub.3) 7.65 (ddd, J=8.0, 4.9, 1.6 Hz, 4H), 7.47-7.31 (m, 6H), 7.19 (dd, J=8.4, 1.7 Hz, 1H), 6.43 (d, J=0.8 Hz, 1H), 4.77 (d, J=1.8 Hz, 2H), 4.40 (d, J=6.3 Hz, 1H), 3.97 (s, OH), 2.31 (ddd, J=8.4, 6.5, 4.4 Hz, 1H), 1.12 (s, 9H), 0.84 (d, J=6.8 Hz, 3H). ESI-MS m/z=536.1 [M+H].sup.+.

[0911] Step C

[0912] 1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-1H-indol-2-yl)methanol (0.175 g, 0.783 mmol) was cooled to 0 C., and NaH (0.094 g, 2.35 mmol, 3 equiv) was added in one portion. The reaction stirred for 30 minutes and propargyl bromide (0.131 mL, 1.17 mmol, 1.5 equiv) was then added. The reaction was quenched with aqueous NH.sub.4Cl and extracted with ethyl acetate. The organics were then dried over MgSO.sub.4, filtered and evaporated to get the crude product. Silica gel chromatography with hexanes and ethyl acetate yielded (R)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-2-((prop-2-yn-1-yloxy)methyl)-1H-indole (0.410 g, 91% yield). .sup.1H NMR (400 MHz, CDCl.sub.3) 7.71-7.59 (m, 4H), 7.59-7.50 (m, 1H), 7.49-7.35 (m, 5H), 7.19 (dd, J=8.4, 1.7 Hz, 1H), 6.49 (d, J=0.8 Hz, 1H), 4.71 (s, 2H), 4.38 (dd, J=14.7, 6.2 Hz, 1H), 4.07 (dd, J=2.3, 1.2 Hz, 2H), 3.61 (dd, J=10.3, 4.7 Hz, 1H), 3.52 (dd, J=10.3, 6.2 Hz, 1H), 2.41 (t, J=2.4 Hz, 1H), 2.32 (ddd, J=8.9, 6.8, 5.1 Hz, 1H), 1.12 (s, 9H), 0.84 (d, J=6.8 Hz, 3H). ESI-MS m/z=574.1 [M+H].sup.+.

[0913] Step D

[0914] (R)-6-bromo-1-(3-((tert-butyldiphenylsilyl)oxy)-2-methylpropyl)-2-((prop-2-yn-1-yloxy)methyl)-1H-indole (0.410 g, 0.7135 mmol) was dissolved in THF (10 mL) and then 1 M TBAF in THF (0.856 mL, 0.856 mmol, 1.2 equiv) was added and the solution stirred for 1 hour. The reaction was quenched with aqueous NH.sub.4Cl and extracted with ethyl acetate. The organics were then dried over MgSO.sub.4, filtered and evaporated to get the crude product. Silica gel chromatography with hexanes and ethyl acetate yielded (R)-3-(6-bromo-2-((prop-2-yn-1-yloxy)methyl)-1H-indol-1-yl)-2-methylpropan-1-ol (0.223 g, 92% yield). .sup.1H NMR (400 MHz, CDCl.sub.3) 7.53-7.49 (m, 1H), 7.44 (d, J=8.4 Hz, 1H), 7.19 (dd, J=8.4, 1.7 Hz, 1H), 6.52 (d, J=0.9 Hz, 1H), 4.86-4.68 (m, 3H), 4.31-4.20 (m, 1H), 4.17 (dd, J=5.5, 2.4 Hz, 2H), 3.96 (dd, J=14.7, 6.5 Hz, 1H), 3.44 (dd, J=6.8, 4.4 Hz, 2H), 2.51 (t, J=2.4 Hz, 1H), 2.34 (dddd, J=9.0, 6.7, 4.4, 2.3 Hz, 1H), 1.04 (d, J=7.0 Hz, 4H). ESI-MS m/z=336.0 [M+H].sup.+.

[0915] Step E

[0916] (R)-3-(6-bromo-2-((prop-2-yn-1-yloxy)methyl)-1H-indol-1-yl)-2-methylpropan-1-ol (0.188 g, 0.559 mmol) was dissolved in THF (5 mL) and cooled to 78 C., 1 M LiHMDS in THF (1.17 mL, 1.17 mmol, 2.1 equiv) was slowly added over 15 minutes. The solution was stirred for 30 minutes at 78 C. TMSCl (0.156 mL, 1.23 mmol, 2.2 equiv) was then added dropwise over 5 minutes and the reaction stirred for 1 hour at 78 C. The reaction was quenched with water and warmed to room temperature. 1 M aqueous HCl and ethyl acetate were added and the reaction stirred for 10 minutes. The solution was extracted 3 with ethyl acetate and dried over MgSO.sub.4, filtered, and evaporated. Silica gel chromatography with hexanes and ethyl acetate provided (R)-3-(6-bromo-2-(((3-(trimethylsilyl)prop-2n-yl)oxy)methyl)-1H-indol-1-yl)-2-methylpropan-1-ol (0.203 g, 89% yield). ESI-MS m/r=408.1 [M+H].sup.+.

BX: (8-bromo-4,5-dihydro-1H,3H-[1,4]oxazepino[4,3-a]indol-4-yl)methanol

[0917] ##STR00322##

[0918] Step A

[0919] (6-bromo-1H-indol-2-yl)methanol (4.0 g, 17.7 mmol) in DMF (165 mL) was treated with NaH (60% dispersion, 1.77, g. 44.2 mmol, 2.5 equiv) in one portion. The resulting mixture was stirred for 30 minutes at room temperature and then treated dropwise with dibromo alkene (2.6 mL, 23 mmol, 1.3 equiv). The resulting solution was stirred at room temperature for 2 hours before being quenched by the addition of water and extracted into ethyl acetate. The combined organic layers were washed with water and brine and then dried (MgSO.sub.4), filtered and concentrated in vacuo. The crude material was purified over silica gel with hexanes/ethyl acetate affording 8-bromo-4-methylene-4,5-dihydro-1H,3H-[1,4]oxazepino[4,3-a]indole (1.65 g, 34% yield) as a white solid. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.52-7.47 (m, 1H), 7.41 (d, J=8.4 Hz, 1H), 7.18 (dd, J=8.4, 1.7 Hz, 1H), 6.34 (t, J=0.8 Hz, 1H), 5.35 (s, 1H), 5.26 (t, J=1.0 Hz, 1H), 4.81 (d, J=0.7 Hz, 2H), 4.73 (d, J=0.7 Hz, 2H), 4.42 (d, J=1.0 Hz, 2H). ESI-MS m/z=278.0 [M+H].sup.+.

[0920] Step B

[0921] 2 M Borane.Me.sub.2S in THF (2.97 mL, 5.93 mmol, 1 equiv) was added cooling on an ice/methanol bath to a stirred solution of 8-bromo-4-methylene-4,5-dihydro-1H,3H-[1,4]oxazepino[4,3-a]indole (1.65 g, 5.93 mmol) in THF (60 mL) under an atmosphere of argon. The reaction mixture was warmed to 25 C. and stirred at this temperature for 2 hours. Then the reaction mixture was cooled in an ice/methanol bath and slowly treated at this temperature sequentially with 3 N sodium hydroxide (2 mL) and 30% hydrogen peroxide (0.788 mL). The obtained homogenous mixture was stirred overnight, then treated with hexane and dried over potassium carbonate. The organic layer was decanted from the precipitate, which was washed with dichloromethane. The organic layers were evaporated in vacuo, and the residue was purified rapidly by chromatography on silica gel to give (8-bromo-4,5-dihydro-1H,3H-[1,4]oxazepino[4,3-a]indol-4-yl)methanol (0.698 g, 40%) as a colorless oil. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.50 (s, 1H), 7.42 (dd, J=8.5, 0.5 Hz, 1H), 7.17 (d, J=6.7 Hz, 1H), 6.40 (s, 1H), 4.82 (d, J=14.3 Hz, 1H), 4.65-4.47 (m, 2H), 4.28 (d, J=14.5 Hz, 1H), 4.12 (dd, J=10.4, 2.2 Hz, 1H), 3.98 (d, J=12.5 Hz, 1H), 3.55 (dd, J=10.5, 5.8 Hz, 1H), 3.41-3.25 (m, 1H), 2.22-2.06 (m, 1H). ESI-MS m/z=296.1 [M+H].sup.+.

BY: 3-(6-bromo-2,3,5,6-tetrahydrospiro[indoline-3,4-pyran]-1-yl)-2,2-dimethylpropan-1-ol

[0922] ##STR00323##

[0923] Step A

[0924] A solution of 6-bromo-1,2-dihydrospiro[indole-3,4-oxan]-2-one (800 mg, 2.836 mmol, 1.0 equiv), (3-bromo-2,2-dimethylpropoxy)(tert-butyl)dimethylsilane (1.20 g, 4.25 mmol, 1.5 equiv), Cs.sub.2CO.sub.3 (2.21 g, 7.10 mmol, 2.5 equiv), and DMF (8.0 mL) was stirred for 13 hours at 130 C. The mixture was cooled to room temperature and then TBAF (1.0 M in THF, 8.5 mL) was added dropwise. The resulting mixture was stirred for an additional 2 hours at room temperature and then diluted with ethyl acetate (50 mL). The organic layer was washed with 340 mL of brine. The organics were dried over sodium sulfate, filtered, and the solvent was removed in vacuo. Purification by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (1:2) gave 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-2,3,5,6-tetrahydrospiro[indoline-3,4-pyran]-2-one (600 mg, 57% yield) as a yellow solid. ESI-MS m/z=368.2 [M+H].sup.+.

[0925] Step B

[0926] A solution of 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-2,3,5,6-tetrahydrospiro[indoline-3,4-pyran]-2-one (600 mg, 1.63 mmol, 1 equiv). THF (6.0 mL) and BH.sub.3.THF (6.0 mL) was stirred at room temperature for 15 hours. The reaction was quenched by the addition of water (20 mL) at 0 C., and the aqueous layer was extracted with ethyl acetate (330 mL). The organics were dried over sodium sulfate, filtered, and the solvent was removed in vacuo. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (1:1) to afford 3-(6-bromo-2,3,5,6-tetrahydrospiro[indoline-3,4-pyran]-1-yl)-2,2-dimethylpropan-1-ol (350 mg, 61% yield) as a yellow solid. ESI-MS m/z=354.2 [M+H].sup.+.

CA: 3-(5-bromo-1-ethyl-1H-indol-3-yl)-2-methylpropan-1-ol

[0927] ##STR00324##

[0928] Step A

[0929] Into a 100 mL round-bottom flask was added 5-bromo-1-ethyl-1H-indole-3-carbaldehyde (5.2 g, 20.63 mmol, 1.0 equiv), methyl 2-(triphenyl-A-5-phosphanylidene)propanoate (17.96 g, 51.57 mmol, 2.5 equiv) and dichloromethane (50 mL). The resulting mixture was stirred for 15 hours at 35 C. The resulting mixture was concentrated under vacuum and then the resulting residue was diluted with ethyl acetate (50 mL) and washed with 350 mL of brine. The organics were dried over sodium sulfate, filtered, and the solvent was removed in vacuo. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (2:1) to afford ethyl 3-(5-bromo-1-ethyl-1H-indol-3-yl)-2-methylacrylate (6.1 g, 90% yield) as a yellow solid. ESI-MS m/z=336.1 [M+H].sup.+.

[0930] Step B

[0931] Into a 250 mL round-bottom flask was added ethyl 3-(5-bromo-1-ethyl-1H-indol-3-yl)-2-methylacrylate (5.70 g, 16.95 mmol, 1.0 equiv), 4-methylbenzene-1-sulfonohydrazide (15.79 g, 84.764 mmol, 5.0 equiv) and DMF (50 mL). The mixture was stirred for 15 hours at 110 C. and was then diluted with ethyl acetate (100 mL). The resulting mixture was washed with 3100 mL of brine and the aqueous layer was extracted with ethyl acetate (350 mL). The organics were dried over sodium sulfate, filtered, and the solvent was removed in vacuo. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (1:2) to afford ethyl 3-(5-bromo-1-ethyl-1H-indol-3-yl)-2-methylpropanoate (3.2 g, 56% yield) as a yellow oil. ESI-MS m/z=338.1 [M+H].sup.+.

[0932] Step C

[0933] Into a 40 mL vial was added ethyl 3-(5-bromo-1-ethyl-1H-indol-3-yl)-2-methylpropanoate (2.00 g, 5.91 mmol, 1.0 equiv), LiBH.sub.4 (515.22 mg, 23.65 mmol, 4.0 equiv) and THF (10 mL). The mixture was stirred for 15 hours at 55 C. The reaction was quenched with saturated aqueous NH.sub.4Cl after cooling to 0 C. The resulting mixture was concentrated under vacuum and the resulting aqueous layer was extracted with ethyl acetate (330 mL). The organics were dried over sodium sulfate, filtered, and the solvent was removed in vacuo. The residue was purified by reverse phase chromatography (20-80% MeCN in water) to give 3-(5-bromo-1-ethyl-1H-indol-3-yl)-2-methylpropan-1-ol (1.7 g, 97% yield) as a yellow oil. ESI-MS m/z=296.1 [M+H].sup.+.

CB: 2-(5-bromo-3-(3-hydroxy-2-methylpropy)-1H-Indol-1-yl)acetonitrile

[0934] ##STR00325##

[0935] Step A

[0936] A solution of tert-butyl 5-bromo-3-formyl-1H-indole-1-carboxylate (13.4 g, 41.3 mmol, 1.0 equiv) in dichloromethane (140 ml) was treated with ethyl 2-(triphenyl-A-5-phosphanylidene)propanoate (37.45 g, 103.3 mmol, 2.5 equiv) and was stirred for 24 hours at 35 C. The solvent was removed and the crude product was purified by silica gel chromatography (PE/EA=10% to 20%) to give 16.4 g (97% yield) of tert-butyl 5-bromo-3-(3-ethoxy-2-methyl-3-oxoprop-1-en-1-yl)-1H-indole-1-carboxylate as a yellow solid. ESI-MS m/z=408.3 [M+H].sup.+.

[0937] Step B

[0938] A solution of tert-butyl 5-bromo-3-[(1 Z)-3-ethoxy-2-methyl-3-oxoprop-1-en-1-yl]-1H-indole-1-carboxylate (16.4 g, 40.2 mmol, 1.0 equiv), DMF (164 mL) and TsNHNH.sub.2 (74.80 g, 401.7 mmol, 10 equiv) was stirred for 5 days at 110 C. After cooling, ethyl acetate (200 mL) was added and the solution was washed with water (3100 mL). The organics were dried over sodium sulfate, filtered, and the solvent was removed in vacuo. The crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate (10% increasing to 20% within 40 minutes)), affording 6.8 g (55% yield) of ethyl 3-(5-bromo-1H-indol-3-yl)-2-methylpropanoate as a yellow oil. ESI-MS m/z=310.1 [M+H].sup.+.

[0939] Step C

[0940] A solution of ethyl 3-(5-bromo-1H-indol-3-yl)-2-methylpropanoate (5.8 g, 18.7 mmol, 1.0 equiv), THF (60 mL) and LiBH.sub.4 (1.63 g, 74.8 mmol, 4.0 equiv) was stirred for 6 hours at 40 C. The reaction was quenched by the addition of 30 mL of saturated aqueous NH.sub.4Cl. The resulting solution was extracted with ethyl acetate (350 mL) and the organic layers were combined. The organics were dried over sodium sulfate, filtered, and the solvent was removed in vacuo. The crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate (35% to 55%)) to give 4.85 g (97% yield) of 3-(5-bromo-1H-indol-3-yl)-2-methylpropan-1-ol as a light yellow oil. ESI-MS m/z=268.1 [M+H].sup.+.

[0941] Step D

[0942] Into a 100 mL 3-necked flask was added 3-(5-bromo-1H-indol-3-yl)-2-methylpropan-1-ol (2.0 g, 7.46 mmol, 1 equiv). DMF (20 mL), and 2,6-dimethylpyridine (3.20 g, 29.834 mmol, 4 equiv) at 20 C. Finally, TBSOTf (5.91 g, 22.375 mmol, 3 equiv) was added dropwise at 20 C., and the resulting solution was stirred for 8 hours at 20 C. The resulting mixture poured into ethyl acetate (100 mL) and washed with H.sub.2O (330 mL). The organic layer was concentrated and the crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate (10% to 20%)) to give 2.2 g (77% yield) of 5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methylpropyl)-1H-indole as a yellow oil. ESI-MS m/z=382.0 [M+H].sup.+.

[0943] Step E

[0944] A solution of 5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methylpropyl)-1H-indole (2.5 g, 6.537 mmol, 1 equiv) in DMF (25 mL) 0 C. was treated with NaH (787 mg, 19.7 mmol, 3.01 equiv, 60% dispersion in mineral oi). After stirring at that temperature for 30 minutes, 2-bromoacetonitrile (1.57 g, 13.1 mmol, 2.00 equiv) was added at 0 C. The resulting solution was stirred for 30 hours at room temperature. The reaction was quenched with ice water and the mixture was extracted with ethyl acetate (350 mL). The combined organic layers were concentrated and the crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate (5% to 15%)) to give 968 mg (35% yield) of 2-(5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methylpropyl)-1H-indol-1-yl)acetonitrile as a yellow oil. .sup.1H NMR (300 MHz, CDCl.sub.3) 7.80 (d, J=1.8 Hz, 1H), 7.40 (dd, J=8.7, 1.9 Hz, 1H), 7.22 (d, J=8.7 Hz, 1H), 6.89 (s, 1H), 4.96 (s, 2H), 4.24-4.08 (m, 1H), 3.56-3.44 (m, 2H), 2.91 (dd, J=14.3, 5.7 Hz, 1H), 2.45 (dd, J=14.3, 8.0 Hz, 1H), 2.07 (s, 1H), 2.03-1.90 (m, 1H), 1.57 (s, 2H), 1.28 (t, J=7.1 Hz, 1H), 0.94 (d, J=11.0 Hz, 14H).

[0945] Step F

[0946] A solution of 2-(5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methylpropyl)-1H-indol-1-yl)acetonitrile (1.0 g, 2.373 mmol, 1 equiv), THF (10 mL), and TBAF (1 M in THF, 4.75 mL) was stirred for 3 hours at room temperature. The solvent was removed in vacuo and the crude product was purified by silica gel (petroleum ether/ethyl acetate (30% to 45%)) to give 460 mg (63% yield) of 2-(5-bromo-3-(3-hydroxy-2-methylpropyl)-1H-indol-1-yl)acetonitrile as a yellow oil. ESI-MS m/z=307.0 [M+H].sup.+.

[0947] The following compounds were synthesized according to the procedure described to make Intermediate CB using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00022 Intermediate No. Structure Analytical Data CB-1 [00326] ESI-MS m/z = 338.1 [M + H]+

CC: 2-(5-bromo-3-(3-hydroxy-2-methylpropyl)-1H-Indol-1-yl)-2-methylpropanenitrile

[0948] ##STR00327##

[0949] Step A

[0950] A solution of 5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methylpropyl)-1H-indole (2.2 g, 5.75 mmol, 1.0 equiv), 2-bromo-2-methylpropanamide (2.87 g, 17.259 mmol, 3 equiv), PPh.sub.3 (301 mg, 1.151 mmol, 0.2 equiv), K.sub.3PO.sub.4 (2.44 g, 11.506 mmol, 2 equiv), NaOH (230 mg, 5.75 mmol, 1.0 equiv), and CuBr(SMe.sub.2) (237 mg, 1.151 mmol, 0.2 equiv) in toluene (12 mL) was stirred for 15 hours at 55 C. The resulting mixture was filtered and the filtrate was concentrated under reduced pressure. The crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate (10% to 40%)) to give 2-(5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methyloxy-2-methylpropyl)-1H-indol-1-yl)-2-methylpropanamide (1.7 g, 63.21% yield) as a light yellow solid. ESI-MS m/z=489.3 [M+Na].sup.+.

[0951] Step B

[0952] A solution of 2-(5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methylpropyl)-1H-indol-1-yl)-2-methylpropanamide (1.7 g, 3.64 mmol, 1.0 equiv) in dichloromethane (34 mL) at 0 C. was treated with Et.sub.3N (1.47 g, 14.55 mmol, 4.0 equiv) and then TFAA (1.91 g, 9.090 mmol, 2.5 equiv). The resulting solution was stirred overnight at room temperature and then the solution was diluted with dichloromethane and washed with water. The organics were dried over magnesium sulfate, filtered, and the solvent was removed in vacuo. The crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate (4% to 15%)), affording 1.15 g (70% yield) of 2-(5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methylpropyl)-1H-indol-1-yl)-2-methylpropanenitrile as a light yellow oil. .sup.1H NMR (300 MHz, CDCl.sub.3-d) 7.78 (d, J=1.9 Hz, 1H), 7.59 (d, J=8.8 Hz, 1H), 7.38 (d, J=8.9 Hz, 1H), 6.97 (s, 1H), 3.48 (t, J=5.9 Hz, 2H), 2.89 (dd, J=14.4, 5.9 Hz, 1H), 2.44 (dd, J=14.3, 7.9 Hz, 1H), 2.06 (d, J=1.8 Hz, 6H), 2.00-1.89 (m, 1H), 0.96 (s, 9H), 0.91 (d, J=6.7 Hz, 3H), 0.09 (s, 6H).

[0953] Step C

[0954] A solution of 2-(5-bromo-3-(3-((tert-butyldimethylsilyl)oxy)-2-methylpropyl)-1H-indol-1-yl)-2-methylpropanenitrile (1.15 g, 2.558 mmol, 1 equiv) in THF (12 mL) at 0 C. was treated with TBAF (1 M in THF, 5.12 mL). The resulting solution was stirred for 4 hours at room temperature. The crude product was purified by silica gel chromatography (petroleum ether/ethyl acetate (10% to 60%)) affording 840 mg (98% yield) of 2-(5-bromo-3-(3-hydroxy-2-methylpropyl)-1H-indol-1-yl)-2-methylpropanenitrile as a yellow oil. ESI-MS m/z=335.1 [M+H].sup.+.

CD: (E)-3-(8-bromo-1-((S)-3-hydroxy-2-methylpropyl)-3-methylindolin-3-yl)acrylonitrile

[0955] ##STR00328##

[0956] Step A

[0957] To a solution of diethyl propanedioate (4.1 mL, 27.3 mmol, 1.2 equiv) in DMF (32 mL) was added potassium carbonate (4.71 g, 34.09 mmol, 1.5 equiv) and 4-bromo-1-fluoro-2-nitro-benzene (2.8 mL, 22.73 mmol, 1.0 equiv) at room temperature and the reaction mixture was stirred for 20 hours at 70 C. Then, the reaction was cooled down to 50 C., and additional potassium carbonate (3.14 g, 22.73 mmol, 1 equiv) and iodomethane (4.24 mL, 68.18 mmol, 3 equiv) were added and stirred 1 hour. The reaction was cooled to room temperature and diluted with water and ethyl aceate. Separated organic layers were washed with saturated brine solution. The organics were then separated and dried (magnesium sulfate) before concentration to dryness. The crude mixture was used without further purification. ESI-MS m/z=374.0 [M+H]*; .sup.1H NMR (400 MHz, CDCl.sub.3) 8.16 (d, J=2.2 Hz, 1H), 7.71 (dd, J=8.5, 2.2 Hz, 1H), 7.23 (d, J=8.5 Hz, 1H), 4.29-4.12 (m, 4H), 1.98 (s, 3H), 1.23 (t, J=7.1 Hz, 6H).

[0958] To a solution of diethyl 2-(4-bromo-2-nitro-phenyl)-2-methyl-propanedioate (8.5 g, 22.7 mmol, 1.0 equiv) in acetic acid (51 mL) was added iron (5.08 g, 90.91 mmol, 4.0 equiv) at room temperature and the reaction mixture was stirred for 1 hour at 95 C. The crude mixture was cooled to room temperature and filtered through a pad of celite and washed with ethyl acetate, concentrated in vacuo. The crude mixture was used without further purification. ESI-MS m/z=298.0 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 10.80 (s, 1H), 7.16 (s, 2H), 7.02 (s, 1H), 4.09-3.99 (m, 2H), 1.48 (s, 3H), 1.04 (t, J=7.0 Hz, 3H).

[0959] Step B

[0960] To a solution of ethyl (3R)-6-bromo-3-methyl-2-oxo-indoline-3-carboxylate (1 g, 3.35 mmol, 1.0 equiv) in DMF (22 mL) was added potassium carbonate (1.39 g, 10.06 mmol, 3.0 equiv), [(2R)-3-bromo-2-methyl-propoxy]-tert-butyl-diphenyl-silane (1.84 g, 4.7 mmol, 1.4 equiv), and NaI (50 mg, 0.34 mmol, 0.1 equiv) at room temperature and the reaction mixture was stirred for 2 days at 65 C. The reaction was cooled down to room temperature and quenched with water and diluted with ethyl acetate. Separated organic layers were washed with brine. The organics were separated and dried (magnesium sulfate) before concentration to dryness. The crude was then purified by flash column chromatography eluting with ethyl acetate and hexanes to give ethyl (3R)-6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-2-oxo-indoline-3-carboxylate (1.2 g, 59% over 3 steps). ESI-MS m/z=630.2 [M+Na].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.72-7.62 (m, 8H), 7.48-7.32 (m, 12H), 7.23-7.16 (m, 2H), 7.17-7.11 (m, 2H), 7.12-7.06 (m, 2H), 4.19-3.97 (m, 4H), 3.87 (dd, J=14.0, 5.3 Hz, 1H), 3.75 (d, J=7.2 Hz, 2H), 3.71-3.60 (m, 2H), 3.58-3.46 (m, 2H), 2.27-2.14 (m, 2H), 1.62 (s, 3H), 1.59 (s, 3H), 1.12 (s. 18H), 1.11-1.07 (m, 6H), 0.91 (d, J=6.8 Hz, 3H), 0.87 (d, J=6.8 Hz, 3H).

[0961] Step C

[0962] To a solution of ethyl (3R)-6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-2-oxo-indoline-3-carboxylate (1.05 g, 1.73 mmol, 1.0 equiv) in THF (3.4 mL) was added borane dimethylsulfide (2 M in THF, 12.9 mL, 25.88 mmol, 15 equiv) at room temperature and the reaction mixture was stirred for 3 hours at 60 C. The reaction mixture was cooled down to room temperature and MeOH was added carefully, dropwise, and stirred until no more gas was observed. The crude mixture was concentrated and purified by silica gel chromatography to give [(3R)-6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indolin-3-yl]methanol (740 mg, 78% yield). ESI-MS m/z=552.3 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.71-7.57 (m, 8H), 7.46-7.31 (m, 12H), 6.87-6.80 (m, 2H), 6.83-6.74 (m, 2H), 6.60 (bs, 2H), 3.68-3.39 (m, 10H), 3.28 (dd, J=13.5, 7.4 Hz, 1H), 3.12 (dd, J=13.5, 6.6 Hz, 1H), 3.10-3.02 (m, 2H), 2.95 (dd, J=13.5, 7.5 Hz, 1H), 2.80 (dd, J=13.5, 6.9 Hz, 1H), 1.26 (s, 6H), 1.08 (s, 18H), 1.00 (d, J=4.3 Hz, 3H), 0.99 (d, J=4.3 Hz, 3H).

[0963] Step D

[0964] To a solution of [(3R)-6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl])-3-methyl-indolin-3-yl]methanol (710 mg, 1.28 mmol) in dichloromethane (16.0 mL) was added Dess-Martin periodinane (708 mg, 1.67 mmol) at room temperature portionwise and the reaction mixture was stirred for 2 hours. The reaction was quenched with sodium bicarbonate (aqueous) and sodium thiosulfate (aqueous) and diluted with dichloromethane. Separated organic layers were washed with brine. The organics were then separated and dried (magnesium sulfate) before concentration to dryness. The crude was then purified by silica gel column chromatography eluting with ethyl acetate and hexanes to give (3R)-6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indoline-3-carbaldehyde (595 mg, 84% yield). ESI-MS m/z=550.1 [M+H].sup.+.

[0965] Step E

[0966] To a solution of 2-diethoxyphosphorylacetonitrile (0.28 mL, 1.73 mmol, 1.6 equiv) in THF (9.1 mL) was added NaH (65 mg, 1.62 mmol, 1.5 equiv, 60% dispersion in mineral oil) at room temperature and the reaction mixture was stirred for 5 minutes. Then (3R)-6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indoline-3-carbaldehyde (595 mg, 1.08 mmol, 1 equiv) in THF (9.0812 mL) was added and stirred at room temperature for 30 minutes. The reaction was quenched with ammonium chloride (aq) and diluted with ethyl acetate. Separated organic layers was washed with saturated brine solution. The organics were then separated and dried (magnesium sulfate) before concentration to dryness. The crude was purified by silica gel column chromatography eluting with ethyl acetate and hexanes to give (E)-3-[(3R)-6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indolin-3-yl]prop-2-enenitrile (430 mg, 69.366%). ESI-MS m/z=573.2 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.68-7.62 (m, 8H), 7.46-7.31 (m, 12H), 6.83-6.76 (m, 2H), 6.75-6.70 (m, 2H), 6.67 (d, J=16.4 Hz, 1H), 6.64-6.58 (m, 2H), 5.20 (d, J=16.5 Hz, 1H), 5.15 (d, J=16.5 Hz, 1H), 3.66-3.51 (m, 5H), 3.31-3.23 (m, 2H), 3.22 (dd, J=13.3, 7.2 Hz, 1H), 3.18-3.09 (m, 2H), 2.89 (dd, J=13.6, 7.2 Hz, 1H), 2.80 (dd, J=13.5, 6.7 Hz, 1H), 1.95 (dtq, J=19.7, 13.4, 7.2 Hz, 2H), 1.37 (s, 3H), 1.35 (s, 3H), 1.11-1.05 (m, 18H), 0.99 (d, J=3.6 Hz, 3H), 0.97 (d, J=3.5 Hz, 3H).

[0967] Step F

[0968] To a solution of (E)-3-[6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indolin-3-yl]prop-2-enenitrile (430 mg, 0.75 mmol, 1.0 equiv) in THF (10.7 mL) was added TBAF (1 M in THF, 0.9 mL, 0.9 mmol, 1.2 equiv) at room temperature and the reaction mixture was stirred for 1 hour at room temperature. The reaction was quenched with ammonium chloride (aq) and diluted with ethyl acetate. Separated organic layers was washed with saturated brine solution. The organics were then separated and dried (magnesium sulfate) before concentration to dryness. The crude was purified by silica gel column chromatography eluting with ethyl acetate and hexanes. The desired fractions were concentrated to dryness in vacuo to give (E)-3-[6-bromo-1-[(2S)-3-hydroxy-2-methyl-propyl]-3-methyl-indolin-3-yl]prop-2-enenitrile (250 mg, 99% yield). ESI-MS m/z=335.1 [M+H].sup.+. .sup.1H NMR (400 MHz, CDCl.sub.3) 6.89-6.85 (m, 2H), 6.81 (d, J=16.5 Hz, 1H), 6.80 (d, J=16.5 Hz, 1H) 6.78 (d, J=1.9 Hz, 1H), 6.76 (d, J=1.9 Hz, 1H), 6.75-6.69 (m, 2H), 5.28 (d, J=16.5 Hz, 1H), 5.24 (d, J=16.5 Hz, 1H), 3.68-3.57 (m, 4H), 3.49 (d, J=9.4 Hz, 1H), 3.40-3.31 (m, 2H), 3.22 (d, J=9.4 Hz, 1H), 3.18 (dd, J=13.7, 8.2 Hz, 1H), 3.11 (dd, J=13.6, 7.7 Hz, 1H), 2.96 (dd, J=13.6, 6.6 Hz, 1H), 2.88 (dd, J=13.6, 6.1 Hz, 1H), 2.11-1.97 (m, 1H), 1.45 (s, 3H), 1.44 (s, 3H), 0.98 (d, J=6.8 Hz, 6H).

CE: 6-bromo-1-((S)-3-hydroxy-2-methylpropyl)-3-methylindoline-3-carbonitrile

[0969] ##STR00329##

[0970] Step A

[0971] To a solution of 6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indoline-3-carbaldehyde (290 mg, 0.53 mmol, 1.0 equiv) in ethanol (5.3 mL) was added NH.sub.2OH.HCl (110 mg, 1.58 mmol, 3.0 equiv) and pyridine (0.21 mL, 2.63 mmol, 5.0 equiv) at room temperature and the reaction mixture was stirred for 1 hour at 50 C. The reaction was cooled to room temperature and diluted with ethyl acetate and aqueous 1 N HCl was added. Separated organic layers were washed with brine. The organics were then separated and dried (magnesium sulfate) before concentration to dryness. The crude mixture was used for the next step without further purification.

[0972] To a solution of (3E)-6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indoline-3-carbaldehyde oxime (298 mg, 0.53 mmol, 1 equiv) in dichloromethane (3.5 mL) was added di(imidazol-1-yl)methanone (171 mg, 1.05 mmol, 2.0 equiv) at room temperature and the reaction mixture was stirred for 20 hours. The crude mixture was concentrated and directly purified by silica gel chromatography to give 6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indoline-3-carbonitrile (255 mg, 88% yield). ESI-MS m/z=547.2 [M+H].sup.+.

[0973] Step B

[0974] To a solution of 6-bromo-1-[(2S)-3-[tert-butyl(diphenyl)silyl]oxy-2-methyl-propyl]-3-methyl-indoline-3-carbonitrile (255 mg, 0.47 mmol, 1.0 equiv) in THF (6.5 mL) was added TBAF (1 M in THF, 0.56 mL, 0.56 mmol, 1.2 equiv) at room temperature and the reaction mixture was stirred for 1 hour at room temperature. The reaction was quenched with ammonium chloride (aq) and diluted with ethyl acetate. Separated organic layers was washed with brine. The organics were then separated and dried (magnesium sulfate) before concentration to dryness. The crude was purified by silica gel chromatography eluting with ethyl acetate and hexanes to give 6-bromo-1-[(2S)-3-hydroxy-2-methyl-propyl]-3-methyl-indoline-3-carbonitrile (105 mg, 73% yield). ESI-MS m/z=309.1 [M+H].sup.+.

CG: tert-butyl ((6.SUP.3.S,4S)-1.SUP.3.-cyano-2.SUP.5.-methoxy-10,10-dimethyl-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-y)carbamate

[0975] ##STR00330##

[0976] tert-Butyl ((6.sup.3S,4S)-1.sup.3-cyano-2.sup.5-hydroxy-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate was synthesized from 6-bromo-1-(3-hydroxy-2,2-dimethylpropyl)-1H-indole-3-carbonitrile using the appropriate intermediates of Example 1 and procedures similar those described in Method A.

[0977] To a stirred solution of tert-Butyl ((6.sup.3S,4S)-1.sup.3-cyano-2.sup.5-hydroxy-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (300 mg, 0.50 mmol, 1.0 equiv) in MeOH/THF (1:4) (5.0 mL) was added TMS-diazomethane (853 g, 7.5 mmol, 15 equiv) at 0 C. The mixture was stirred for 24 hours at room temperature. The reaction mixture was concentrated in vacuo and diluted with ethyl acetate (50 mL) and water (50 mL). The layers were separated and the organic layer was washed with water (230 mL), brine (20 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure. The residue was purified by reverse phase chromatography (formic acid in MeCN/Water) to give tert-butyl ((6.sup.3S,4S)-1.sup.3-cyano-2.sup.5-methoxy-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (180 mg, 60% yield) as a white solid. ESI-MS m/z=616.1 [M+H].sup.+.

CH: methyl (S)-1-((S)-3-(1-(3-acetoxypropyl)-3-(tetrahydro-2H-pyran-4-yl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate

[0978] ##STR00331##

[0979] Methyl (S)-1-((S)-3-(3-(1-(3-acetoxypropyl)-3-(3,6-dihydro-2H-pyran-4-yl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)-2-aminopropanoyl)hexahydropyridazine-3-carboxylate was synthesized from 3-(6-bromo-3-(3,6-dihydro-2H-pyran-4-yl)-1H-indol-1-yl)propyl acetate using conditions similar to those described in Method B.

[0980] A solution of methyl (S)-1-((S)-3-(3-(1-(3-acetoxypropyl)-3-(3,6-dihydro-2H-pyran-4-yl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)-2-aminopropanoyl)hexahydropyridazine-3-carboxylate (230 mg, 0.227 mmol, 1 equiv) in by MeOH (10 mL) was treated with Pd/C (10% on carbon, 50 mg). The mixture was purged H.sub.2 three times and then stirred under an atmosphere of hydrogen for 15 hours. The solids were filtered off and the solvent was removed in vacuo to give methyl (S)-1-((S)-3-(3-(1-(3-acetoxypropyl)-3-(tetrahydro-2H-pyran-4-yl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate (202 mg) that was carried on without further purification. ESI-MS m/z=863.6 [M+H].sup.+.

CI: tert-butyl ((6.SUP.3.S,4S,10S)-1.SUP.3.-bromo-10-methyl-5,7-dioxo-2.SUP.5.-((triisopropylsilyl)oxy)-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate

[0981] ##STR00332##

[0982] Step A

[0983] A solution of 6-iodo-1H-indole (7.00 g, 28.80 mmol, 1.0 equiv), [(2R)-3-bromo-2-methylpropoxy](tert-butyl)diphenylsilane (12.40 g, 31.68 mmol, 1.1 equiv), and Cs.sub.2CO.sub.3 (23.46 g, 72.00 mmol, 2.5 equiv) in DMF was stirred for 14 hours at 80 C. After concentration of the reaction mixture, the residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (8:1) to afford (S)-3-(6-iodo-1H-indol-1-yl)-2-methylpropan-1-ol (4.0 g, 44% yield) as a colorless oil. ESI-MS m/z=316.0 [M+H].sup.+.

[0984] Step B

[0985] To a solution of (S)-3-(6-iodo-1H-indol-1-yl)-2-methylpropan-1-ol (4.00 g, 12.69 mmol, 1.0 equiv) in DMF (40.0 ml) was added NBS (2.48 g, 13.96 mmol, 1.1 equiv) in DMF (10 mL) dropwise at 0 C. The solution was maintained at that temperature for 1 hour. Water was added and the solution was extracted with ethyl acetate (3100 mL). After concentration, the residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (5:1) to afford (S)-3-(3-bromo-6-iodo-1H-indol-1-yl)-2-methylpropan-1-ol (3.2 g, 60% yield) as a yellow oil. ESI-MS m/z=392.0 [MH].sup..

[0986] Step C

[0987] To a stirred solution of (S)-3-(3-bromo-6-iodo-1H-indol-1-yl)-2-methylpropan-1-ol (3.20 g, 4.64 mmol, 1.0 equiv) and (2S)-3-(3-bromo-6-iodo-1H-indol-1-yl)-2-methylpropan-1-ol (1.83 g, 4.64 mmol, 1.0 equiv) in dioxane (25 mL) and H.sub.2O (5.0 mL) was added K.sub.2CO.sub.3 (1.60 g, 11.60 mmol, 2.5 equiv) and Pd(DTBPF)Cl.sub.2 (0.30 g, 0.46 mmol, 0.1 equiv) portionwise. The solution was stirred for 3 hours at 50 C. and then concentrated. The residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (4:1) to afford methyl (S)-1-((S)-3-(3-(3-bromo-1-((S)-3-hydroxy-2-methylpropyl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate (2.9 g, 75% yield) as an oil. ESI-MS m/z=829.4 [M+H].sup.+.

[0988] Step D

[0989] To a stirred solution of methyl (S)-1-((S)-3-(3-(3-bromo-1-((S)-3-hydroxy-2-methylpropyl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate (2.90 g, 3.49 mmol, 1.0 equiv) in DCE (30 mL) was added trimethylstannanol (3.16 g, 17.47 mmol, 5.0 equiv) dropwise. After stirring for 14 hours at 60 C., the solution was concentrated and the residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (2:1) to afford (S)-1-((S)-3-(3-(3-bromo-1-((S)-3-hydroxy-2-methylpropyl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylic acid (2.7 g, 95% yield) as an oil. ESI-MS m/z=815.3 [M+H].sup.+.

[0990] Step E

[0991] To a stirred solution of (S)-1-((S)-3-(3-(3-bromo-1-((S)-3-hydroxy-2-methylpropyl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylic acid (4.00 g, 4.90 mmol, 1.0 equiv) and DIEA (15.84 g, 122.56 mmol, 25.0 equiv) in dichloromethane (40 mL) was added HOBT (3.97 g, 29.42 mmol, 6.0 equiv) and EDCl (15.04 g, 78.439 mmol, 16 equiv) in portions at 0 C. After stirring for 16 hours at room temperature, the solution was concentrated and the residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (5:1) to afford tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-bromo-10-methyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (2.3 g, 59% yield) as a brown solid. ESI-MS m/z=797.3 [M+H].sup.+

CJ: tert-butyl ((6.SUP.3.S,4S,10S)-1.SUP.3.-(3-hydroxy-2-methylbutan-2-yl)-10-methyl-5,7-dioxo-2.SUP.5.-((triisopropylsilyl)oxy)-6.SUP.1.,6.SUP.2.6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-Indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate

[0992] ##STR00333##

[0993] tert-Butyl ((6.sup.3S,4S,10S)-10-methyl-1.sup.3-(2-methyl-3-oxobutan-2-yl)-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate was synthesized from (S)-3-(6-bromo-1-(3-hydroxy-2-methylpropyl)-1.sup.H-indol-3-yl)-3-methylbutan-2-one using the appropriate intermediates of Example 1 and procedures similar those described in Method A.

[0994] To a stirred solution of tert-butyl ((6.sup.3S,4S,10S)-10-methyl-1.sup.3-(2-methyl-3-oxobutan-2-yl)-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (400 mg, 0.498 mmol, 1.0 equiv) in MeOH (10 mL) at 0 C. was added NaBH.sub.4 (75 mg, 1.99 mmol, 4.0 equiv) in portions. The mixture was stirred for 4 hours at room temperature and then water (200 mL) was added. The resulting mixture was extracted with EA (2200 mL). The combined organic layers were washed with water (2100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-(3-hydroxy-2-methylbutan-2-yl)-10-methyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.46.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (350 mg, 70% yield) was used in the next step directly without further purification. ESI-MS m/z=805.4 [M+H].sup.+.

CK: tert-butyl-((6.SUP.3.S,4S,10S)-1.SUP.3.-((E)-1-(methoxyimino)ethyl)-10-methyl-5,7-dioxo-2.SUP.5.-((triisopropylsilyl)oxy)-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate

[0995] ##STR00334##

[0996] tert-Butyl ((6.sup.3S,4S,10S)-1.sup.3-acetyl-10-methyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate was synthesized from (S)-1-(6-bromo-1-(3-hydroxy-2-methylpropyl)-1H-indol-3-yl)ethan-1-one using the appropriate intermediates of Example 1 and procedures similar those described in Method A.

[0997] tert-Butyl ((6.sup.3S,4S,10S)-1.sup.3-acetyl-10-methyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (350 mg, 0.46 mmol, 1.0 equiv) was dissolved in MeOH (3.0 mL) and O-methylhydroxylamine hydrochloride (385 mg, 4.6 mmol, 10 equiv) was added. The mixture was stirred at 15 C. for 10 minutes and then NaHCO.sub.3 (386 mg, 4.6 mmol, 10 equiv) was added in portions. The reaction mixture was stirred for 8 hours. After filtration and concentration, the crude product was purified by silica gel chromatography (Petroleum/ethyl acetate=1:1) to give tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-((E)-1-(methoxyimino)ethyl)-10-methyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (330 mg, 90% yield) as a white solid. ESI-MS m/z=790.4 [M+Na].sup.+.

CL: tert-butyl ((6.SUP.3.S,4S)-2.SUP.5.-hydroxy-10,10-dimethyl-1-(1-methylpiperidin-4-yl)-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate

[0998] ##STR00335##

[0999] tert-Butyl ((6.sup.3S,4S)-2.sup.5-hydroxy-10,10-dimethyl-1.sup.3-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate was synthesized from 3-(6-bromo-3-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-1H-indazol-1-yl)-2,2-dimethylpropan-1-ol using procedures similar to the ones described for the synthesis of (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.sup.3S,4S)-2.sup.5-hydroxy-1.sup.2,10,10-trimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)-3-methylbutanamide.

[1000] A solution of tert-butyl ((6.sup.3S,4S)-2.sup.5-hydroxy-10,10-dimethyl-1-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (78 mg, 0.12 mmol, 1 equiv) and Pd/C (50 mg, 0.47 mmol, 4.05 equiv) in MeOH (1 mL) was purged with H.sub.2 three times. The resulting solution was stirred for 2 hours under an atmosphere of H.sub.2 The solids were filtered off and the resulting mixture was concentrated to give tert-butyl ((6.sup.3S,4S)-2.sup.5-hydroxy-10,10-dimethyl-1.sup.3-(1-methylpiperidin-4-yl)-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (55 mg) as a dark yellow oil that was carried on without further purification. ESI-MS m/z=675.5 [M+H].sup.+.

CM: tert-butyl ((6.SUP.3.S,4S)-1.SUP.3.-ethynyl-2.SUP.5.-hydroxy-10,10-dimethyl-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-Indazola-6(1,3)-pyridazine-2(1,3)-benzenacycloundecaphane-4-yl)carbamate

[1001] ##STR00336##

[1002] tert-Butyl ((6.sup.3S,4S)-10,10-dimethyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-1.sup.3-((trimethylsilyl)ethynyl)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate was synthesized from 3-(6-bromo-3-((trimethylsilyl)ethynyl)-1H-indazol-1-yl)-2,2-dimethylpropan-1-ol using a protocols similar to the ones described for the synthesis of (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.sup.3S,4S)-2.sup.5-hydroxy-1.sup.2,10,10-trimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)-3-methylbutanamide.

[1003] A solution of tert-butyl ((6.sup.3S,4S)-10,10-dimethyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-1.sup.3-((trimethylsilyl)ethynyl)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (220 mg, 0.26 mmol, 1.0 equiv) in THF (3 mL) at 0 C. was treated with TBAF (1 M, 0.132 mL, 0.13 mmol, 0.5 equiv). The resulting solution was stirred for 2 hours at 0 C. The resulting mixture was concentrated. The residue was purified by silica gel chromatography with ethyl acetate/petroleum ether (3/1) to give tert-butyl ((6.sup.3S,4S)-1.sup.3-ethynyl-2.sup.5-hydroxy-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (150 mg, 85% yield) of as a light yellow solid. ESI-MS m/z=602.3 [M+H].sup.+.

CN: tert-butyl ((6.SUP.3.S,4S,10S)-1.SUP.3.-(2-cyanoethyl)-2-hydroxy-13,10-dimethyl-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-8-oxa-1(6,1)-indolina-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate

[1004] ##STR00337##

[1005] ter-Butyl ((6.sup.3S,4S,10S)-1.sup.3-((E)-2-cyanovinyl)-2.sup.5-hydroxy-13,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-8-oxa-1(6,1)-indolina-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate was synthesized from (E)-3-(6-bromo-1-((S)-3-hydroxy-2-methylpropyl)-3-methylindolin-3-yl)acrylonitrile using the appropriate intermediates of Example 1 and procedures similar those described in Method A.

[1006] To a solution of tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-((E)-2-cyanovinyl)-2.sup.5-hydroxy-13,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-8-oxa-1(6,1)-indolina-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate was synthesized from (E)-3-(6-bromo-1-((S)-3-hydroxy-2-methylpropyl)-3-methylindolin-3-yl)acrylonitrile (73 mg, 0.12 mmol, 1.0 equiv) in THF (2.3 mL) was added Pd/C (10 wt %, 37 mg, 0.03 mmol, 0.3 equiv) and stirred for 20 hours under 1 atm of H.sub.2. The crude mixture was filtered through a pad of celite and concentrated in vacuo. The residue was purified by silica gel chromatography with ethyl acetate and hexanes to give tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-(2-cyanoethyl)-2.sup.5-hydroxy-13,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-8-oxa-1(6,1)-indolina-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (51 mg, 70% yield). ESI-MS m/z=654.2 [M+Na].sup.+.

CO: (S)-2-(3-acryloyl-2-oxoimidazolidin-yl)-3-methylbutanoic Acid

[1007] ##STR00338##

[1008] Step A

[1009] To a solution of benzyl N-(2-oxoethyl)carbamate (11.88 g, 1.2 equiv) in methanol (120 mL) was added tert-butyl (2S)-2-amino-3-methylbutanoate hydrochloride (10.70 g, 1 equiv). After cooling to 0 C. sodium cyanoborohydride (9.6 g, 3.0 equiv) was added portionwise while maintaining the reaction temperature between 0 C., and 10 C. To the resulting mixture was added acetic anhydride (3.1 g, 1.0 equiv). The resulting solution was stirred for 4 hours at 25 C. The reaction was then quenched by the addition of 200 mL of ice water. The resulting solution was extracted with dichloromethane (3200 mL). The organics were washed with brine (300 mL), dried over anhydrous sodium sulfate and concentrated under vacuum. The crude product was purified by C.sub.18 reverse phase chromatography (85-95% acetonitrile in water with 0.1% formic acid) to give desired product (5 g). ESI-MS m/z=351.2 [M+H].sup.+.

[1010] Step B

[1011] To a solution of tert-butyl (2-(((benzyloxy)carbonyl)amino)ethyl)-L-valinate (2.4 g, 6.9 mmol) in methanol (10 mL) was added 10% palladium on carbon (1.2 g) under nitrogen. The suspension was degassed under vacuum and purged with hydrogen three times. The mixture was stirred under hydrogen for 14 hours. The reaction mixture was filtered and concentrated to give the desired product (74% yield) as a white solid, which was used for next step reaction without purification. Calculated MW: 216.2; ESI-MS m/z=217 [M+H].sup.+.

[1012] Step C

[1013] tert-Butyl (2-aminoethyl)-L-valinate (1.1 g, 5.09 mmol) was dissolved in DMF (10 mL) and treated with bis(4-nitrophenyl) carbonate (1.9 g, 1.2 equiv). The resulting solution was stirred for 14 hours at 60 C. The reaction mixture was cooled to room temperature and 50 mL of ice water was added. The resulting solution was extracted with ethyl acetate (250 mL) and the organics were washed with water (2100 ml), dried, and concentrated. The residue was purified by silica gel column chromatography (0-10% methanol in dichloromethane) to give the desired product (49% yield) as a yellow solid. ESI-MS m/z=243.1 [M+H].sup.+.

[1014] Step D

[1015] To a mixture of tert-butyl (S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-3-methylbutanoate (0.05 g, 206 umol) in dichloromethane (1 mL) at 0 C. was added triethylamine (57 uL, 410 umol) followed by acryloyl chloride (20 uL, 250 umol). The mixture was stirred for 1 hour and then diluted with dichloromethane (2 mL), washed with water (2 mL) and brine (4 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure at 15 C. to give the crude product as a yellow oil. The crude product was purified by silica gel chromatography (15-25% ethyl acetate in petroleum ether) to give the desired product (83% yield) as a white solid. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.61 (dd, J=10.5, 17.1 Hz, 1H), 6.49 (dd, J=2.0, 17.0 Hz, 1H), 5.79 (dd, J=2.0, 10.4 Hz, 1H), 4.21 (d, J=9.7 Hz, 1H), 3.98-3.76 (m, 3H), 3.47 (dt, J=6.5, 9.1 Hz, 1H), 2.25-2.13 (m, 1H), 1.53-1.45 (m, 9H), 1.04 (d, J=6.6 Hz, 3H), 0.96 (d, J=6.6 Hz, 3H).

[1016] Step E

[1017] To a solution of tert-butyl (S)-3-methyl-2-(2-oxoimidazolidin-1-yl)butanoate (0.05 g, 168 umol) in dichloromethane (0.5 mL) was added trifluoroacetic acid (0.5 mL) at 0 C. After stirring at room temperature for 2 hours, the reaction mixture was concentrated in vacuo to give the desired product (0.041 g) as a yellow solid, which was used without further purification.

CP: (S)-3-methyl-2-(2-oxo-3-(vinylsulfonyl)imidazolidin-1-yl)butanoic Acid

[1018] ##STR00339##

[1019] Step A

[1020] To a solution of tert-butyl (S)-3-methyl-2-(2-oxoimidazolidin-1-yl)butanoate (80 mg, 0.33 mmol, 1.0 equiv) in dichloromethane (4 mL) was added pyridine (1 mL) and ethenesulfonyl chloride (54 mg, 0.43 mmol, 1.3 equiv) at 0 C. The reaction solution was stirred at 0 C. for 2 hours and then concentrated under vacuum to give the crude product that was used without purification. ESI-MS m/z=333.1 [M+H].sup.+.

[1021] Step B

[1022] tert-Butyl (S)-3-methyl-2-(2-oxo-3-(vinylsulfonyl)imidazolidin-1-yl)butanoate (60 mg, 0.18 mmol, 1.0 equiv) was treated with a mixture of trifluoroacetic acid (2 mL) and dichloromethane (4 mL) at 0 C. The reaction solution was stirred at 0 C. for 2 hours. The residue was concentrated under vacuum to give the crude product that was without further purification.

CQ: (S)-3-methyl-2-(2-oxo-4-(vinylsulfonyl)piperazin-1-yl)butanoic Acid

[1023] ##STR00340##

[1024] Step A

[1025] tert-butyl glycyl-L-valinate (2.3 g, 10.0 mmol, 1.0 equiv) and triethylamine (3.03 g, 30.0 mmol, 3.0 equiv) in dichloromethane (30 ml) at 0 C. was added 2-nitrobenzene-1-sulfonyl chloride (2.43 g, 11.0 mmol, 1.1 equiv). The resulting solution was stirred for 2 hours at room temperature. After cooling to 0 C. the resulting mixture was quenched with ice water (30 mL). The mixture was extracted with dichloromethane (260 mL). The organic layers were combined and washed with water (240 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated. Purification by silica gel chromatography (20% ethyl acetate in petroleum ether) afforded the desired product (84% yield) as a colorless oil. ESI-MS m/z=416.1 [M+H].sup.+. .sup.1H NMR (300 MHz, CDCl.sub.3) 8.16-8.13 (m, 1H), 7.96-7.93 (m, 1H), 7.81-7.76 (m, 2H), 6.67 (d, J=6.0 Hz, 1H), 6.26-6.22 (m, 1H), 4.43-4.38 (m, 1H), 3.86 (d, J=6.0 Hz. 2H), 2.19-2.12 (m, 1H), 1.48 (s, 9H), 0.92-0.88 (m, 6H).

[1026] Step B

[1027] To a solution of tert-butyl ((2-nitrophenyl)sulfonyl)glycyl-L-valinate (3.8 g, 9.15 mmol, 1.0 equiv) in DMF (50 mL) at 0 C. was added 1,2-dibromoethane (17.0 g, 91.5 mmol, 10.0 equiv) and potassium carbonate (12.6 g, 91.5 mmol, 10.0 equiv). The mixture was stirred at 50 C. for 18 hours and then poured into ice water (60 mL) and extracted with ethyl acetate (2120 mL). The organic layers were combined and washed with water (50 ml2), dried, filtered, concentrated, and purified by silica gel chromatography (30% ethyl acetate in petroleum ether) to give the desired product (60% yield) as a yellow oil. ESI-MS m/z=442.1 [M+H].sup.+.

[1028] Step C

[1029] A solution of tert-butyl (S)-3-methyl-2-(4-((2-nitrophenyl)sulfonyl)-2-oxopiperazin-1-yl)butanoate (2.3 g, 5.21 mmol, 1.0 equiv), potassium carbonate (3.6 g, 10.42 mmol, 2.0 equiv), and thiophenol (1.15 g, 10.42 mmol, 5.0 equiv) in DMF (30 mL) was stirred for 4 hours. After filtering off the solids and concentration of the filtrate, purification by silica gel chromatography provided the desired product (90% yield) as a clear oil. .sup.1H NMR (300 MHz, CDCl.sub.3) 4.9 (d, J=9.0 Hz, 1H), 3.63 (s, 2H), 3.54-3.48 (m, 1H), 3.31-3.25 (m, 1H), 3.11-3.05 (m, 2H), 2.51-2.35 (m, 1H), 1.48 (s, 9H), 1.15-0.85 (m, 6H).

[1030] Step D

[1031] To a solution of tert-butyl (S)-3-methyl-2-(2-oxopiperazin-1-yl)butanoate (300 mg, 1.17 mmol, 1.0 equiv) in dichloromethane (10 mL) was added diisopropylethylamine (453 mg, 3.1 mmol, 3.0 equiv) and ethenesulfonyl chloride (221 mg, 1.75 mmol, 1.5 equiv) at 0 C. The reaction solution was stirred at 0 C. for 2 hours and then the mixture was concentrated in vacuo. The residue was purified by reverse phase HPLC (5-95% acetonitrile in water with 0.05% formic acid) to give the desired product (40% yield) as a light yellow solid. ESI-MS m/z=347.4 [M+H].sup.+. .sup.1H NMR (300 MHz, CD3OD) 6.79-6.69 (m, 1H), 6.32-6.18 (m, 2H), 4.62 (d, J=12.0 Hz, 1H), 3.90 (d, J=3.0 Hz, 2H), 3.68-3.40 (m, 4H), 2.31-2.23 (m, 1H), 1.49 (s, 9H), 1.07-0.91 (m, 6H).

[1032] Step E

[1033] A solution of tert-butyl (S)-3-methyl-2-(2-oxo-4-(vinylsulfonyl)piperazin-1-yl)butanoate (50 mg, 0.16 mmol, 1.0 equiv) and trifluoroacetic acid (1 mL) in dichloromethane (3 mL) at 0 C. was stirred for 2 hours. The solution was concentrated under vacuum to give the title compound and was used without further purification.

[1034] The following intermediates were synthesized according to the procedure described to make Intermediate CQ using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00023 Intermediate No. Structure Analytical Data CQ-1 [00341] ESI-MS m/z = 255.2 [M + H] CQ-2 [00342] ESI-MS m/z = 277.2 [M + H]+

CR: Synthesis of tert-butyl (S)-2-(R)-3-amino-2-oxopyrrolidin-1-yl)-3-methylbutanoate

[1035] ##STR00343##

[1036] Step A

[1037] To a stirred solution of (2R)-2-[[(benzyloxy)carbonyl]amino]-4-(methylsulfanyl)butanoic acid (15 g, 52.940 mmol, 1 equiv) in DMF (200 mL) at 0 C. was added tert-butyl (2S)-2-amino-3-methylbutanoate hydrochloride (12.21 g, 58.2 mmol, 1.10 equiv), DIEA (17.11 g, 132.3 mmol, 2.5 equiv) and HATU (24.16 g, 63.5 mmol, 1.2 equiv). The resulting mixture was stirred for 2 hours at 0 C., and then diluted with water (1 L). The resulting mixture was extracted with ethyl acetate (2500 mL). The combined organic layers were washed with water (2500 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to give a residue that was purified by silica gel column chromatography, eluting with EA/PE (1:5-1:4) to afford tert-butyl ((benzyloxy)carbonyl)-D-methionyl-L-valinate (20 g, 78% yield) as a colorless oil. ESI-MS m/z=439.3 [M+H].sup.+.

[1038] Step B

[1039] To a stirred solution of tert-butyl ((benzyloxy)carbonyl)-D-methionyl-L-valinate (20 g, 45.602 mmol, 1 equiv) in acetone (200 mL) was added iodomethane (30 mL). The resulting mixture was stirred for 48 hours at room temperature and then concentrated under reduced pressure. To a mixture of the crude product in acetonitrile (200 mL) was added Cs.sub.2CO.sub.3 (44.57 g, 136.8 mmol, 3.0 equiv). After stirring for 4 hours at 60 C., water (1 L) was added. The resulting mixture was extracted with ethyl acetate (2500 mL). The combined organic layers were washed with water (2500 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by reverse phase chromatography (70-75% acetonitrile in water with 0.1% FA) to afford tert-butyl (S)-2-((R)-3-(((benzyloxy)carbonyl)amino)-2-oxopyrrolidin-1-yl)-3-methylbutanoate (11 g, 55.60% yield) as a yellow oil. ESI-MS m/z=391.3 [M+H].sup.+.

[1040] Step C

[1041] To a stirred solution of tert-butyl (2S)-2-[(3R)-3-[[(benzyloxy)carbonyl]amino]-2-oxopyrrolidin-1-yl]-3-methylbutanoate (1.5 g, 3.841 mmol, 1 equiv) in ethyl acetate (15 mL) was added Pd/C (10%, 500 mg, 4.7 mmol, 1.22 equiv) in portions. The solution was purged with H.sub.2 and stirred under a hydrogen atmosphere for 16 hours at 40 C. The resulting mixture was filtered and the filter cake was washed with ethyl acetate (310 mL). The filtrate was concentrated under reduced pressure to give tert-butyl (2S)-2-[(3R)-3-amino-2-oxopyrrolidin-1-yl]-3-methylbutanoate (800 mg, 73% yield) as a light-yellow oil. ESI-MS m/z=257.2 [M+H].sup.+.

CS: tert-butyl (S)-3-methyl-2-((R)-3-(methylamino)-2-oxopyrrolidin-1-yl)butanoate

[1042] ##STR00344##

[1043] Step A

[1044] To a stirred solution of tert-butyl (S)-2-((R)-3-(((benzyloxy)carbonyl)amino)-2-oxopyrrolidin-1-yl)-3-methylbutanoate (1.5 g, 3.841 mmol, 1 equiv) in THF (15 mL) was added NaH (232 mg, 5.8 mmol, 1.5 equiv, 60%) in several batches at 0 C. The resulting mixture was stirred for 1 hour at 0 C., and then iodomethane (821 mg, 5.784 mmol, 1.51 equiv) was added dropwise. The resulting mixture was stirred for additional 1.5 hours at 0 C., and then aqueous saturated NH.sub.4Cl (10 mL) was added and concentrated under vacuum. The resulting mixture was diluted with water (100 mL) and extracted with ethyl acetate (3200 mL). The combined organic layers were washed with water (2100 mL) and brine (100 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to give tert-butyl (S)-2-((R)-3-(((benzyloxy)carbonyl)(methyl)amino)-2-oxopyrrolidin-1-yl)-3-methylbutanoate (1.6 g, 87% yield) as a light-yellow oil. ESI-MS m/z=405.3 [M+H].sup.+.

[1045] Step B

[1046] To a stirred solution of give tert-butyl (S)-2-((R)-3-(((benzyloxy)carbonyl)(methyl)amino)-2-oxopyrrolidin-1-yl)-3-methylbutanoate (800 mg, 1.978 mmol, 1 equiv) in ethyl acetate (15 mL) was added Pd/C (10 mol %, 320 mg, 3.007 mmol, 1.52 equiv) in portions. The solution was purged with hydrogen gas and stirred for 24 hours at 40 C. under an atmosphere of hydrogen. The suspension was filtered and the filter cake was washed with ethyl acetate (310 mL). The filtrate was concentrated under reduced pressure to give tert-butyl (S)-3-methyl-2-((R)-3-(methylamino)-2-oxopyrrolidin-1-yl)butanoate (460 mg, 73% yield) as a light-yellow oil. ESI-MS m/z=271.2 [M+H].sup.+.

CT: tert-butyl N-(azetidine-3-carbonyl)-N-ethyl-L-valinate

[1047] ##STR00345##

[1048] Step A

[1049] A solution of N-((benzyloxy)carbonyl)-N-ethyl-L-valine (880 mg, 3.15 mmol, 1.0 equiv) and tert-butyl 3,3,3-trichloro-2-iminopropanoate (3.09 g, 12.53 mmol, 4.0 equiv) in THF/dichloromethane (1:4, 10 mL) was stirred for 6 days at room temperature. The crude product was purified by reverse phase chromatography with the following conditions (0% MeCN to 100% MeCN) to afford tert-butyl N-((benzyloxy)carbonyl)-N-ethyl-L-valinate (520 mg, 49% yield) as a yellow oil. ESI-MS m/z=336.4 [M+H].sup.+.

[1050] Step B

[1051] A solution of tert-butyl N-((benzyloxy)carbonyl)-N-ethyl-L-valinate (500 mg, 1.49 mmol, 1.0 equiv) and Pd/C (10%, 50 mg, 0.47 mmol, 0.3 equiv) in MeOH (5.0 mL) was stirred for 3 hours at room temperature under an atmosphere of hydrogen. The mixture was filtered through celite. The filtrate was concentrated to give tert-butyl ethyl-L-valinate (270 mg, 90% yield) as an oil. .sup.1H NMR (300 MHz, DMSO-d.sub.6) 2.77 (d, J=6.2 Hz, 1H), 2.65-2.52 (m, 1H), 2.38 (t, J=9.2 Hz, 1H), 1.78 (dt, J=13.3, 6.8 Hz, 1H), 1.43 (t, J=1.5 Hz, 9H), 0.99 (t, J=7.1 Hz, 3H), 0.88 (dt, J=6.2, 2.7 Hz, 6H).

[1052] Step C

[1053] A solution of tert-butyl ethyl-L-valinate (265 mg, 1.32 mmol, 1.0 equiv), DIEA (510 mg, 3.95 mmol, 3.0 equiv), 1-[(benzyloxy)carbonyl]azetidine-3-carboxylic acid (371 mg, 1.58 mmol, 1.2 equiv) and HATU (751 mg, 1.98 mmol, 1.5 equiv) in CH.sub.2Cl.sub.2 (3.0 mL) was stirred for 1 hour at 0 C. The residue was purified directly by Prep-TLC (PE/ethyl acetate 1:1) to afford benzyl (S)-3-((1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl)(ethyl)carbamoyl)azetidine-1-carboxylate (500 mg, 91% yield) as a yellow oil. ESI-MS m/z=419.4 [M+H].sup.+.

[1054] Step D

[1055] A solution of benzyl (S)-3-((1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl)(ethyl)carbamoyl)azetidine-1-carboxylate (460 mg, 1.10 mmol, 1.0 equiv) and Pd/C (10%, 150 mg, 1.41 mmol, 1.28 equiv) in ethyl acetate/MeOH (1:1, 8 mL) was stirred for 3 hours at room temperature under an atmosphere of H.sub.2. The resulting mixture was filtered through celite. The filtrate was concentrated under reduced pressure to give tert-butyl N-(azetidine-3-carbonyl)-N-ethyl-L-valinate (300 mg, 96% yield) as a yellow oil ESI-MS m/z=285.2 [M+H].sup.+.

CU: tert-butyl (S)-3-cyclobutyl-2-(methylamino)propanoate

[1056] ##STR00346##

[1057] Step A

[1058] To a stirred solution of (2S)-2-amino-3-cyclobutylpropanoic acid (3.0 g, 20.9 mmol, 1 equiv) in THF (30 mL) and H.sub.2O (30 mL) at 0 C. was added sodium bicarbonate (5.28 g, 62.9 mmol, 3.0 equiv) and benzyl 2,5-dioxopyrrolidin-1-yl carbonate (7.83 g, 31.4 mmol, 1.5 equiv) in portions. The mixture was stirred for 16 hours at room temperature and then acidified to pH 5 with aqueous 1N HCl. The resulting mixture was extracted with ethyl acetate (3150 mL). The combined organic layers were washed with brine and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (2:1) to afford (S)-2-(((benzyloxy)carbonyl)amino)-3-cyclobutylpropanoic acid (5.4 g, 74% yield) as a yellow oil. ESI-MS m/z=278.1 [M+H].sup.+.

[1059] Step B

[1060] A solution of (S)-2-(((benzyloxy)carbonyl)amino)-3-cyclobutylpropanoic acid (5.4 g, 19.5 mmol, 1 equiv), toluene (55 mL), paraformaldehyde (5.84 g, 194.721 mmol, 10.0 equiv) and TsOH (0.34 g, 1.95 mmol, 0.10 equiv) was stirred for 16 hours at 100 C. The resulting mixture was filtered and the filtrate was concentrated under vacuum. The residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (4:1) to afford benzyl (S)-4-(cyclobutylmethyl)-5-oxooxazolidine-3-carboxylate (2.5 g, 35% yield) as a yellow oil. .sup.1H-NMR (400 MHz, DMSO-d.sub.6) 7.45-7.35 (m, 5H), 5.45 (d, J=4.1 Hz, 1H), 5.28 (d, J=4.2 Hz, 1H), 5.24-5.06 (m, 2H), 4.27 (t. J=5.3 Hz, 1H), 2.32 (h, J=8.3 Hz, 1H), 2.10-1.90 (m, 3H), 1.89-1.46 (m, 5H).

[1061] Step C

[1062] To a stirred solution of benzyl (S)-4-(cyclobutylmethyl)-5-oxooxazolidine-3-carboxylate (2.5 g, 8.641 mmol, 1 equiv) in trichloromethane (30 mL) at 0 C. was added Et.sub.3SiH (6.98 mL) followed by TFA (15 mL) dropwise. The resulting mixture was stirred for 16 hours at room temperature and then concentrated under vacuum. The residue was purified by Prep-TLC (PE/ethyl acetate 2:1) to afford (S)-2-(((benzyloxy)carbonyl)(methyl)amino)-3-cyclobutylpropanoic acid (2.2 g, 79% yield) as a yellow oil. ESI-MS m/z=292.2 [M+H].sup.+.

[1063] Step D

[1064] (S)-2-(((Benzyloxy)carbonyl)(methyl)amino)-3-cyclobutylpropanoic acid (2.2 g, 7.551 mmol, 1 equiv) and tert-butyl 3,3,3-trichloro-2-iminopropanoate (14.89 g, 60.409 mmol, 8 equiv) in THF (5 mL) was stirred for 2 days at room temperature. The resulting mixture was concentrated under reduced pressure. The resulting mixture was filtered and the filter cake was washed with dichloromethane (350 mL). The filtrate was concentrated under reduced pressure and then purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (4:1) to afford tert-butyl (S)-2-(((benzyloxy)carbonyl)(methyl)amino)-3-cyclobutylpropanoate (2.3 g, 70% yield) as a yellow oil. ESI-MS m/z=370.2 [M+Na]

[1065] Step E

[1066] A solution of tert-butyl (S)-2-(((benzyloxy)carbonyl)(methyl)amino)-3-cyclobutylpropanoate (2.3 g, 6.620 mmol, 1 equiv) in toluene (30 mL) was treated with Pd/C (500 mg, 5% on carbon). The solution was purged with hydrogen and the reaction mixture was stirred for 16 hours at room temperature under an atmosphere of hydrogen. The resulting mixture was filtered and the filter cake was washed with ethyl acetate (3100 mL). The filtrate was concentrated under reduced pressure to give tert-butyl (S)-3-cyclobutyl-2-(methylamino)propanoate (1.9 g, crude) as a yellow oil. The crude product was used in the next step directly without further purification. ESI-MS m/z=214.3 [M+H].sup.+.

[1067] The following intermediates were synthesized according to the procedure described to make Intermediate CU using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00024 Intermediate No. Structure Analytical Data CU-1 [00347] ESI-MS m/z = 174.2 [M + H]+ CU-2 [00348] ESI-MS m/z = 200.2 [M + H]+

CV: N-(1-(2-chloroacetyl)azetidine-3-carbonyl)-N-methyl-L-valine (PH-SF-42H)

[1068] ##STR00349##

[1069] Step A

[1070] To a stirred solution of tert-butyl (2S)-3-methyl-2-(methylamino)butanoate (1.5 g, 8.01 mmol, 1.0 equiv) and DIEA (2.1 g, 16.02 mmol, 2.0 equiv) in dichloromethane (15 mL) was added 1-[(benzyloxy)carbonyl]azetidine-3-carboxylic acid (1.9 g, 8.01 mmol, 1 equiv) and CIP (3.3 g, 12.01 mmol, 1.5 equiv) in portions at 0 C. The resulting mixture was stirred for 2 hours at room temperature and the solution was then concentrated under reduced pressure. The residue was purified by prep-TLC (petroleum ether/ethyl acetate 5:1 w/0.1% TEA) to afford benzyl 3-[[(2S)-1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl](methyl)carbamoyl] azetidine-1-carboxylate(1.2 g, 37%) as a colorless oil. ESI-MS m/z=427.40 [M+Na].sup.+.

[1071] Step B

[1072] To a solution of benzyl 3-[[(2S)-1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl](methyl)carbamoyl]azetidine-1-carboxylate (1.2 mg, 1 equiv) in acetonitrile (20 mL) was added Pd/C (10%, 120 mg) under a nitrogen atmosphere. The mixture was stirred under an atmosphere of hydrogen for 4 h, filtered through celite, and concentrated under reduced pressure. The crude product was used in the next step directly without further purification. ESI-MS m/z=271.20 [M+H].sup.+

[1073] Step C

[1074] To a solution of tert-butyl N-(azetidine-3-carbonyl)-N-methyl-L-valinate (6 g, 22.19 mmol, 1.0 equiv) in dichloromethane (50 mL) at 0 C. was added triethylamine (3.4 g, 33.29 mmol, 1.5 equiv) followed by dropwise addition of 2-chloroacetyl chloride (2.8 g, 24.41 mmol, 1.10 equiv). The resulting solution was stirred for 1 hour at 0 C., and then the mixture was concentrated under vacuum. The residue was purified by reverse phase chromatography (10-50% MeCN in water with 0.1% FA) to give tert-butyl N-(1-(2-chloroacetyl)azetidine-3-carbonyl)-N-methyl-L-valinate (2.03 g, 26% yield) as a light brown oil. .sup.1H-NMR (300 MHz, DMSO-d.sub.6) 4.57-4.51 (m, 1H), 4.42-4.37 (m, 1H), 4.35-4.24 (m, 1H), 5.30-4.12 (s, 3H), 3.96-3.81 (m, 2H), 2.81-2.76 (m, 3H), 2.17-2.08 (m, 1H), 1.42 (s, 9H), 0.95 (d, J=6.6 Hz, 3H), 0.79 (d, J=4.4 Hz, 3H).

[1075] Step D

[1076] A solution of tert-butyl N-(1-(2-chloroacetyl)azetidine-3-carbonyl)-N-methyl-L-valinate (200 mg, 0.58 mmol, 1 equiv) in dichloromethane (4 mL), at 0 C. was treated with trifluoroacetic acid (2 mL). The resulting solution was stirred for 30 min at 0 C., and then 3 hours at room temperature. The resulting mixture was concentrated to give N-(1-(2-chloroacetyl)azetidine-3-carbonyl)-N-methyl-L-valine (220 mg) as a crude solid that was used without further purification. ESI-MS m/z=291.1 [M+H].sup.+.

[1077] The following compounds were synthesized according to the procedure described to make Intermediate CV using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00025 Intermediate No. Structure Analytical Data CV-1 [00350] ESI-MS m/z = 269.1 [M + H]+ CV-2 [00351] ESI-MS m/z = 305.2 [M + H]+. CV-3 [00352] ESI-MS m/z = 305.2 [M + H]+ CV-4 [00353] ESI-MS m/z = 305.14 [M + H]+ CV-5 [00354] ESI-MS m/z = 283.2 [M + H]+ CV-6 [00355] ESI-MS m/z = 283.2 [M + H]+ CV-7 [00356] ESI-MS m/z = 437.2 [M + H]+ CV-8 [00357] ESI-MS m/z = 263.1 [M + H]+ CV-9 [00358] CV-10 [00359] ESI-MS m/z = 291.1 [M + H]+ CV-11 [00360] ESI-MS m/z = 269.1 [M + H]+ CV-12 [00361] ESI-MS m/z = 305.1 [M + Na]+ CV-13 [00362] ESI-MS m/z = 305.2 [M + H]+ CV-14 [00363] ESI-MS m/z = 305.1 [M + H]+ CV-15 [00364] ESI-MS m/z = 291.1 [M + H]+ CV-16 [00365] ESI-MS m/z = 317.2 [M + H]+ CV-17 [00366] ESI-MS m/z = 277.1 [M + H]+ CV-18 [00367] ESI-MS m/z = 303.1[M + H]+ CV-19 [00368] ESI-MS m/z = 305.1 [M + H]+; .sup.1H NMR (300 MHz, DMSO-d.sub.6) 13.1 (s, 0.5H), 12.5 (s, 0.5H), 4.39- 4.28 (m, 2H), 4.24-4.01 (m, 3H), 3.94-3.79 (m, 2H), 3.64-3.61 (m, 1H), 3.28-3.22 (m, 2H), 2.25-2.15 (m, 1H), 1.12-0.96 (m, 6H), 0.81- 0.76 (m, 3H). CV-20 [00369] ESI-MS m/z = 277.1 [M + H]+ CV-21 [00370] ESI-MS m/z = 355.0 [M + H]+

CW: N-(1-(but-2-ynoyl)azetidine-3-carbonyl)-N-methyl-L-valine

[1078] ##STR00371##

[1079] Step A

[1080] A solution of tert-butyl N-(azetidine-3-carbonyl)-N-methyl-L-valinate (1 g, 3.70 mmol, 1 equiv), MeCN (10 mL), DIEA (1.4 g, 11.10 mmol, 3 equiv), but-2-ynoic acid (373.1 mg, 4.44 mmol, 1.2 equiv), and CIP (1.5 g, 5.55 mmol, 1.5 equiv) was stirred for 1 hour at 0 C. The solvent was removed and the crude product was purified by silica gel chromatography (petroleum ether/ethyl aceate (2:3)), to give tert-butyl N-(1-(but-2-ynoyl)azetidine-3-carbonyl)-N-methyl-L-valinate (1.0 g, 80% yield) as a brown oil. ESI-MS m/z=337.4 [M+H].sup.+.

[1081] Step B

[1082] N-(1-(but-2-ynoyl)azetidine-3-carbonyl)-N-methyl-L-valine was prepared from tert-butyl N-(1-(but-2-ynoyl)azetidine-3-carbonyl)-N-methyl-L-valinate using a procedure similar to the one described for the synthesis of N-(1-(2-chloroacetyl)azetidine-3-carbonyl)-N-methyl-L-valine. ESI-MS m/z=281.2 [M+H].sup.+.

[1083] The following intermediates were synthesized according to the procedure described to make Intermediate CW using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00026 Intermediate No. Structure Analytical Data CW-1 [00372] ES-MS m/z = 295.2 [M + H]+ CW-2 [00373] ES-MS m/z = 295.2 [M + H]+ CW-3 [00374] ES-MS m/z = 335.1 [M + H]+

CX: N-(1-acryloyl-3-fluoroazetidine-3-carbonyl)-N-methyl-valine

[1084] ##STR00375##

[1085] Step A

[1086] To a solution of 1-[(tert-butoxy)carbonyl]-3-fluoroazetidine-3-carboxylic acid (220 mg, 1.004 mmol, 1.0 equiv), tert-butyl (2S)-3-methyl-2-(methylamino)butanoate (225.55 mg, 1.204 mmol, 1.2 equiv) and DIEA (389 mg, 3.01 mmol, 3 equiv) in acetonitrile (3.0 mL) at 0 C. was added HATU (763 mg, 2.01 mmol, 2 equiv). The resulting solution was stirred for 2 hours at room temperature. The solution was diluted with 100 mL of ethyl acetate. The layers were separated and the aqueous layer was washed with 250 ml of NH.sub.4Cl and 250 mL of brine. The resulting mixture was concentrated under vacuum. The crude product was purified by Prep-HPLC (5-95% acetonitrile in water with 0.1% FA) to give tert-butyl (S)-3-((1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl)(methyl)carbamoyl)-3-fluoroazetidine-1-carboxylate (360 mg 92% yield) as a brown oil. ESI-MS m/z=411.2 [M+Na].sup.+.

[1087] Step B

[1088] A solution of tert-butyl (S)-3-((1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl)(methyl)carbamoyl)-3-fluoroazetidine-1-carboxylat (360 mg, 0.927 mmol, 1.0 equiv) and TFA (2 mL) in dichloromethane (4 mL) was stirred at 0 C. for 2 h. The solid was concentrated in vacuo to give N-(3-fluoroazetidine-3-carbonyl)-N-methyl-L-valine (220 mg) (crude) as a brown oil. ESI-MS m/z=233.4 [M+H].sup.+.

[1089] Step C

[1090] To a solution of N-(1-acryloyl-3-fluoroazetidine-3-carbonyl)-N-methyl-L-valine (220 mg, 0.947 mmol, 1.0 equiv) and DIEA (367.27 mg, 2.842 mmol, 3 equiv) in dichloromethane (4.0 mL) at 0 C., was added prop-2-enoyl chloride (103 mg, 1.14 mmol, 1.2 equiv). The resulting solution was stirred for 1 hour at 0 C. The mixture was concentrated under vacuum. The crude product was purified by Prep-HPLC (5-95% water in acetonitrile with 0.1% FA) to give N-(1-acryloyl-3-fluoroazetidine-3-carbonyl)-N-methyl-L-valine (37% yield) as a light yellow oil. ESI-MS m/z=287.1 [M+H].sup.+.

[1091] The following intermediate was synthesized according to the procedure described to make Intermediate CX using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00027 Intermediate No. Structure Analytical Data CX-1 [00376] ES1-MS m/z = 295.2 [M + H]+

CY: N-((R)-1-acryloylazetidine-2-carbonyl)-N-methyl-L-valine

[1092] ##STR00377##

[1093] Step A

[1094] To a solution of tert-butyl (R)-azetidine-2-carboxylate in ethyl acetate (30 mL) was added dropwise acryloyl chloride (2.2 g, 25 mmol, 1.0 equiv) at 0 C. The reaction mixture was stirred for 10 minutes at 0 C., and then water was added. The layers were separated and the organic layer was washed with brine (10 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure. The residue was purified by column chromatography on silica gel (33-50% ethyl acetate in petroleum ether) to give the tert-butyl (R)-1-acryloylazetidine-2-carboxylate (3.5 g) as a white oil. ESI-MS n/z=212.1 [M+H].sup.+.

[1095] Step B

[1096] To a solution of tert-butyl (R)-1-acryloylazetidine-2-carboxylate (3.5 g, 16.5 mmol, 1.0 equiv) in dichloromethane (16.0 mL) was added TFA (48.0 mL) at 20 C. The resulting solution was stirred at 20 C. for 1 hour. The solvent was removed under reduced pressure to give (R)-1-acryloylazetidine-2-carboxylic acid (4.0 g) as a white solid. This crude product was used in the next step without further purification. ESI-MS m/z=156.1 [M+H].sup.+.

[1097] Step C

[1098] To a stirred solution of (R)-1-acryloylazetidine-2-carboxylic acid (4.0 g crude, 13.2 mmol, 1.0 equiv) and tert-butyl methyl-L-valinate (5 g, 26.4 mmol, 2.0 equiv) in DMF (30 mL) was added DIEA (8 g, 66 mmol, 5.0 equiv) followed by HATU (7.4 mg, 19.8 mmol, 1.5 equiv) at 20 C. The resulting solution was stirred for 1 hour. The solution was diluted with ethyl acetate (100 mL) and water (40 mL). Layer were separated and the organic layer was washed with water (330 mL), brine (30 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure. The residue was purified by reverse phase chromatography (0.1% formic acid in MeCN/Water) to give the tert-butyl N-((R)-1-acryloylazetidine-2-carbonyl)-N-methyl-L-valinate (1.2 g, P: 98%) as a white solid. ESI-MS m/z=325.2 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) 6.33 (ddd, J=17.0, 10.3, 2.9 Hz, 0.5H), 6.17-5.77 (m, 2H), 5.69 (dd, J=10.3, 1.7 Hz, 0.5H), 5.62-5.42 (m, 1.5H), 5.33-5.06 (m, 0.5H), 4.42 (dd, J=10.3, 3.7 Hz, 0.5H), 4.12 (dt, J=11.3, 6.3 Hz, 1H), 3.96-3.75 (m, 1.5H), 3.69 (d, J=10.4 Hz, 0.5H), 2.91 (d, J=15.0 Hz, 0.5H), 2.83 (s, 1H), 2.75 (d, J=13.1 Hz, 0.5H), 2.72 (s, 1.5H), 2.16 (ddd, J=10.3, 8.7, 5.3 Hz, 1H), 2.10-1.89 (m, 1H), 1.41 (d, J=4.6 Hz, 9H), 0.95 (t, J=5.8 Hz, 3H), 0.86 (dd, J=6.6, 3.4 Hz, 1.5H), 0.82-0.73 (m, 1.5H).

[1099] Step D

[1100] A solution of tert-butyl N-((R)-1-acryloylazetidine-2-carbonyl)-N-methyl-L-valinate (38 mg, 0.116 mmol, 1.0 equiv) in dichloromethane (1.0 mL) was treated with TFA (0.5 mL) at 20 C. The resulting solution was stirred at 20 C. for 1 hour. The solvent was removed under reduced pressure to give N-((R)-1-acryloylazetidine-2-carbonyl)-N-methyl-L-valine (40 mg) as a yellow oil. This crude product was used in the next step without further purification. ESI-MS m/z=269.1 [M+H].sup.+.

[1101] The following compounds were synthesized according to the procedure described to make Intermediate CY using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00028 Intermediate No. Structure Analytical Data CY-1 [00378] ESI-MS m/z = 269.2 [M + H]+ CY-2 [00379] ESI-MS m/z = 283.2 [M + H]+. CY-3 [00380] ESI-MS m/z = 295.2 [M + H]+. CY-4 [00381] CY-5 [00382] ESI-MS m/z = 281.2 [M + H]+ CY-6 [00383] ESI-MS m/z = 281.2 [M + H]+ CY-7 [00384] ESI-MS m/z: 295.1

CZ: (R)-3-methyl-2-(vinylsulfonamidomethyl)butanoic Acid

[1102] ##STR00385##

[1103] Step A

[1104] To a stirred solution of tert-butyl methyl-L-valinate (350 mg, 1.86 mmol, 1.0 equiv) and [(benzyloxy)carbonyl]glycine (587 mg, 2.8 mmol, 1.5 equiv) in DMF (5 mL) at 0 C. was added DIEA (2400 mg, 18.7 mmol, 10 equiv) dropwise at 0 C. After 5 minutes, COMU (1600 mg, 3.7 mmol, 2 equiv) was added in portions over the course of 5 min. The resulting mixture was stirred for 2 hours at 0 C. The resulting mixture was diluted with water (50 mL) and extracted with ethyl acetate (320 mL). The combined organics were dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by C.sub.18 reverse phase chromatography (0% MeCN to 100% MeCN in water with 0.05% FA) to give tert-butyl N-(((benzyloxy)carbonyl)glycyl)-N-methyl-L-valinate (469 mg, 66% yield) as an orange oil. ESI-MS m/z=401.3 [M+Na].sup.+.

[1105] Step B

[1106] A solution of tert-butyl N-(((benzyloxy)carbonyl)glycyl)-N-methyl-L-valinate (459 mg, 1.21 mmol, 1.0 equiv) and Pd/C (150 mg, 1.41 mmol, 1.16 equiv) in ethyl acetate (5 mL) was stirred for 4 hours at room temperature under a hydrogen atmosphere. The resulting mixture was filtered, the filter cake was washed with ethyl acetate (320 mL). The filtrate was concentrated under reduced pressure to afford tert-butyl glycyl-L-valinate (281 mg, 95% yield) as a yellow oil. The crude product was used in the next step directly without further purification. ESI-MS m/z=231.3 [M+H].sup.+.

[1107] Step C

[1108] To a stirred solution of tert-butyl glycyl-L-valinate (120 mg, 0.49 mmol, 1.0 equiv) and TEA (149 mg, 1.47 mmol, 3.0 equiv) in dichloromethane (2.5 mL) at 0 C. was added 2-chloroacetyl chloride (83 mg, 0.73 mmol, 1.50 equiv) dropwise. The resulting mixture was stirred for 2 hours at 0 C. After filtration, the mixture was concentrated under reduced pressure. The residue was purified by C.sub.18 reverse phase chromatography (0% MeCN to 100% MeCN with NH.sub.4HCO.sub.3, 0.5%) to give tert-butyl N-((2-chloroacetyl)glycyl)-N-methyl-L-valinate (104 mg, 66% yield) as a brown oil. ESI-MS m/z=321.2 [M+H].sup.+.

[1109] Step D

[1110] To a stirred solution of tert-butyl N-((2-chloroacetyl)glycyl)-N-methyl-L-valinate (100 mg, 0.31 Mmol, 1.0 equiv) in dichloromethane (0.8 mL) was added TFA (0.5 mL) dropwise at 0 C. After 2 hours, the resulting mixture was concentrated under reduced pressure. The residue was diluted with 5 mL toluene, and concentrated under vacuum again. The above procedure was repeated for another one additional time and (R)-3-methyl-2-(vinylsulfonamidomethyl)butanoic acid (148 mg) was obtained as a brown oil that was used in the next step directly without purification. ESI-MS m/z=265.2 [M+H].sup.+.

DA: (S)-2-(2-acryloyl-5-oxo-2,6-diazaspiro[3.4]octan-6-yl)-3-methylbutanoic Acid

[1111] ##STR00386##

[1112] Step A

[1113] To a stirred mixture of 1-tert-butyl 3-methyl 3-(2-oxoethyl)azetidine-1,3-dicarboxylate (1.4 g, 5.44 mmol, 1.0 equiv), tert-butyl (2S)-2-amino-3-methylbutanoate hydrochloride (1.37 g, 6.530 mmol, 1.2 equiv) and ZnCl.sub.2 (0.74 g, 5.441 mmol, 1 equiv) in MeOH (30 mL) was added NaBH.sub.3CN (0.34 g, 5.44 mmol, 1.0 equiv) at 0 C. After stirring for 2 hours, the reaction was quenched with water (50 mL) at 0 C. and the methanol was removed under in vacuo. The resulting mixture was extracted with ethyl acetate (350 mL) and the combined organic layers were washed with brine (330 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to give 1-tert-butyl 3-methyl 3-(2-[[(2S)-1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl]amino]ethyl)azetidine-1,3-dicarboxylate (1.2 g, 53% yield) as an oil. The crude product mixture was used in the next step directly without further purification. .sup.1H NMR (300 MHz, CDCl.sub.3) 4.32-4.07 (m, 4H), 3.76 (s, 4H), 2.88 (d, J=5.6 Hz, 1H), 2.69 (ddd, J=11.8, 7.5, 5.6 Hz, 1H), 2.55-2.40 (m, 1H), 2.26-2.06 (m, 2H), 1.91 (dq, J=13.2, 6.7 Hz, 1H). 1.48 (s, 9H), 1.45 (s, 10H), 0.94 (d, J=6.8 Hz, 6H).

[1114] Step B

[1115] A solution of 1-tert-butyl 3-methyl 3-(2-[[(2S)-1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl]amino]ethyl)azetidine-1,3-dicarboxylate (1.2 g, 2.89 mmol, 1.0 equiv) and LiOHH.sub.2O (607 mg, 14.5 mmol, 5.0 equiv) in MeOH/H2O (5/1) (40 mL) was stirred for 1 hour. The methanol was removed under reduced pressure and the aqueous was diluted with ethyl acetate (50 mL). The layers were separated and the organics were washed with 325 mL of brine and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure and the crude product 3-(2-[[(2S)-1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl]amino]ethyl)-1-[(tert-butoxy)carbonyl]azetidine-3-carboxylic acid was used in the next step directly without further purification. .sup.1H NMR (300 MHz, DMSO-d.sub.6) =4.01 (d, J=8.4 Hz, 3H), 3.73 (d, J=11.8 Hz, 4H), 2.85 (s, 2H), 2.25 (dd, J=18.1, 6.8 Hz, 1H), 1.48 (s, 8H), 1.39 (d, J=1.5 Hz, 13H), 1.04 (d, J=6.9 Hz, 3H), 0.94 (d, J=6.8 Hz, 3H).

[1116] Step C

[1117] A solution of 3-(2-[[(2S)-1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl]amino]ethyl)-1-[(tert-butoxy)carbonyl]azetidine-3-carboxylic acid (1.2 g, 2.99 mmol, 1.0 equiv), COMU (2.56 g, 6.0 mmol, 2.0 equiv) and DIEA (1.94 g, 15 mmol, 5.0 equiv) in DMF (30 mL) was stirred for 1 hour at 0 C. The mixture was diluted with water (80 mL) and the resulting mixture was extracted with ethyl acetate (350 mL). The combined organic layers were washed with brine (350 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse phase chromatography with the following conditions (10% to 50% acetonitrile in water. 25 min gradient) to afford tert-butyl 6-[(2S)-1-(tert-butoxy)-3-methyl-1-oxobutan-2-yl]-5-oxo-2,6-diazaspiro[3.4]octane-2-carboxylate (800 mg, 70% yield) as a yellow solid. .sup.1H NMR (300 MHz, CDCl.sub.3) 4.39 (d, J=9.6 Hz, 1H), 4.22 (dd, J=10.4, 8.3 Hz, 2H), 3.78 (dd, J=8.3, 3.5 Hz, 2H), 3.62 (dt, J=10.0, 6.6 Hz, 1H), 3.31 (dt, J=10.0, 6.8 Hz, 1H), 2.39-2.23 (m, 2H), 2.18 (dp, J=9.6, 6.7 Hz, 1H), 1.46 (d, J=4.0 Hz, 17H), 1.01 (d, J=6.7 Hz, 3H), 0.88 (d, J=6.8 Hz, 3H).

[1118] Step D

[1119] A solution of tert-butyl 6-[(2S)-3-methyl-1-oxo-1-(propan-2-yloxy)butan-2-yl]-5-oxo-2,6-diazaspiro[3.4]octane-2-carboxylate (200 mg, 0.543 mmol, 1.0 equiv) and TFA (2 mL) in dichloromethane (2 mL) was stirred for 1 hour. The resulting mixture was concentrated under vacuum to afford (2S)-3-methyl-2-[5-oxo-2,6-diazaspiro[3.4]octan-6-yl]butanoic acid (120 mg, 98% yield). The crude product was carried on without further purification. .sup.1H NMR (300 MHz, DMSO-d.sub.6) 9.00 (s, 1H), 8.87 (s, 1H), 4.18 (d, J=9.8 Hz, 1H), 3.99 (s, 1H), 3.50-3.37 (m, 1H), 3.31 (dt, J=9.8, 6.8 Hz, 1H), 2.36 (t, J=6.8 Hz, 2H), 2.21-2.03 (m, 1H), 0.94 (d, J=6.6 Hz, 3H), 0.80 (d, J=6.7 Hz, 3H).

[1120] Step E

[1121] To a stirred solution of (2S)-3-methyl-2-[5-oxo-2,6-diazaspiro[3.4]octan-6-yl]butanoic acid (120 mg, 0.53 mmol, 1.0 equiv) and DIEA (342 mg, 2.65 mmol, 5.0 equiv) in dichloromethane (3 mL) was added prop-2-enoyl chloride (144 mg, 1.59 mmol, 3.0 equiv) at 0 C., and then maintained for 3 hours at that temperature. The resulting mixture was concentrated under vacuum and purified by reverse phase chromatography (10% to 25% gradient in 30 min) to afford (2S)-3-methyl-2-[5-oxo-2-(prop-2-enoyl)-2,6-diazaspiro[3.4]octan-6-yl]butanoic acid (140 mg, 94% yield) as a white solid. .sup.1H NMR (300 MHz, DMSO-d.sub.6) =6.32 (ddd, J=17.0, 10.2, 1.9 Hz, 1H), 6.11 (dd, J=17.0, 2.3 Hz, 1H), 5.74-5.63 (m, 1H), 4.30-4.09 (m, 2H), 4.09-3.78 (m, 4H), 3.65 (s, 1H), 3.22 (d, J=8.1 Hz, 1H), 2.20 (tq, J=24.8, 9.4, 7.7 Hz, 2H), 0.93 (d, J=6.5 Hz, 3H), 0.76 (d, J=6.7 Hz, 3H).

[1122] The following intermediates were synthesized according to the procedure described to make Intermediate DA using appropriate building blocks and modified reaction conditions (such as reagents, ratio of reagents, temperature, and reaction time) as needed.

TABLE-US-00029 Intermediate No. Structure Analytical Data DA-1 [00387] ESI-MS m/z = 303.1 [M + H]+ DA-2 [00388] ESI-MS m/z = 317.1 [M + H]+

DB: (S)-2-(2-acryloyl-oxo-2,6-diazaspiro[3.4]octan-6-yl)-3-methylbutanoic Acid

[1123] ##STR00389##

[1124] Step A

[1125] To a stirred solution of (2S)-2-(methylamino)propanoic acid (600 mg, 5.82 mmol, 1.0 equiv) and DIEA (2.26 g, 17.46 mmol, 3.0 equiv) in dichloromethane (50 mL) was added benzyl 3-(carboxy)azetidine-1-carboxylate (1.77 g, 6.982 mmol, 1.2 equiv) dropwise at 0 C. The resulting mixture was stirred for 2 hours at that temperature and then the mixture was diluted with dichloromethane (100 mL). The combined organic layers were washed with water (350 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by reverse phase chromatography (0-100% MeCN in water with 0.05% FA) to afford (2S)-2-(1-[1-[(benzyloxy)carbonyl]azetidin-3-yl]-N-methylformamido)propanoic acid (700 mg, 22% yield) as a light yellow oil. ESI-MS m/z=321.2 [M+H].sup.+.

[1126] Step B

[1127] A solution of (2S)-2-(1-[1-[(benzyloxy)carbonyl]azetidin-3-yl]-N-methylformamido)propanoic acid (700 mg, 2.185 mmol, 1 equiv) in methanol (50 mL) was treated with Pd/C (100 mg, 5% on carbon). Hydrogen gas was bubbled through the solution and then stirred for an additional 3 hours under an atmosphere of hydrogen. The resulting mixture was filtered and the filter cake was washed with MeOH (230 mL). The filtrate was concentrated under reduced pressure and the crude product was used in the next step directly without further purification. ESI-MS m/z=187.1 [M+H].sup.+.

[1128] Step C

[1129] To a stirred solution of (2S)-2-[1-(azetidin-3-yl)-N-methylformamido]propanoic acid (400 mg. 1.074 mmol, 1 equiv) and DIEA (416 mg, 3.222 mmol, 3.0 equiv) in THF (30 mL) was added 2-chloroacetyl chloride (145 mg, 1.29 mmol, 1.2 equiv) dropwise at 0 C. The resulting mixture was stirred for 2 hours at 20 C., and the resulting mixture was concentrated under vacuum. The residue was purified by reverse phase chromatography (0-100% MeCN in water with 0.05% FA) to afford (2S)-2-[1-[1-(2-chloroacetyl)azetidin-3-yl]-N-methylformamido]propanoic acid (100 mg, 24.81% yield) as a yellow oil. ESI-MS m/z=263.2 [M+H].sup.+.

DC: N-(2-(3,4-dimethyl-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-ylacetyl)-N-methyl-L-valine

[1130] ##STR00390##

[1131] Step A

[1132] A solution of dimethyl-2,5-dihydrofuran-2,5-dione (2.0 g, 15.86 mmol, 1.0 equiv), 2-aminoacetic acid (1.2 g, 15.86 mmol, 1.0 equiv) and acetic acid (20 mL) was irradiated with microwave radiation for 2 hours at 120 C. The crude product was purified by C18 reverse phase chromatography (2-4% MeCN in water (10 mmol/L TFA)) to give 2-(3,4-dimethyl-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)acetic acid (1.06 g, 34%) as a light yellow solid. ESI-MS m/z=182.0 [MH].sup.+.

[1133] Step B

[1134] Into a 40-mL vial was placed 2-(3,4-dimethyl-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)acetic acid (200 mg, 1.09 mmol, 1.0 equiv), dichloromethane (2.0 mL), DIEA (0.9 mL, 6.98 mmol, 5 equiv), tert-butyl (2S)-3-methyl-2-(methylamino)butanoate (245 mg, 1.31 mmol, 1.2 equiv) and HATU (830 mg, 2.18 mmol, 2.0 equiv). The resulting solution was stirred for 2 hours at 0 C., and then the resulting mixture was concentrated. The residue was purified by silica gel chromatography with ethyl acetate/petroleum ether (1/1) to give tert-butyl (2S)-2-[2-(3,4-dimethyl-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-N-methylacetamido]-3-methylbutanoate (260 mg, 68% yield) as a yellow oil. ESI-MS m/z=353.3 [M+H].sup.+.

[1135] Step C

[1136] A solution of tert-butyl (2S)-2-[2-(3,4-dimethyl-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-N-methylacetamido]-3-methylbutanoate (35 mg), dichloromethane (1.5 mL), and TFA (1 mL) was stirred for 2 hours at 0 C. The resulting mixture was concentrated to give 30 mg of (2S)-2-[2-(3,4-dimethyl-2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-N-methylacetamido]-3-methylbutanoic acid as a yellow oil. ESI-MS m/z=297.2 [M+H].sup.+.

Example 2Synthesis of (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.SUP.3.S,4S)-2.SUP.5.-hydroxy-1.SUP.2.,10,10-trimethyl-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)-3-methylbutanamide (Compound 1)

[1137] ##STR00391## ##STR00392##

[1138] Step A

[1139] A solution of methyl (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoyl)hexahydropyridazine-3-carboxylate (1.0 g, 1.4 mmol, 1.0 eq), 3-(6-bromo-2-methyl-1H-benzo[d]imidazol-1-yl)-2,2-dimethylpropan-1-ol (430 mg, 1.4 mmol, 1.0 eq), Pd(dppf)Cl.sub.2 (100 mg, 10 mol %), and K.sub.2CO.sub.3 (500 mg, 3.6 mmol, 2.5 eq) in dioxane (30 mL) was stirred at 75 C. for 16 hours. After concentration, the residue was purified by silica gel column chromatography (petroleum ether/ethyl acetate (5/1 to 1/3)) to afford methyl (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(1-(3-hydroxy-2,2-dimethylpropyl)-2-methyl-1H-benzo[d]imidazol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoyl)hexahydropyridazine-3-carboxylate (330 mg, 27%) as a light yellow solid. ESI-MS m/z=780.5 [M+H].sup.+.

[1140] Step B

[1141] A solution of methyl (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(1-(3-hydroxy-2,2-dimethylpropyl)-2-methyl-1H-benzo[d]imidazol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoyl)hexahydropyridazine-3-carboxylate (450 mg, 0.58 mmol, 1.0 eq in, dichloroethane (5.0 mL) was treated with trimethyltin hydroxide (522 mg, 2.88 mmol, 5.0 equiv). The resulting solution was stirred for 6 hours at 60 C. After concentration, the crude product was diluted with ethyl acetate (20 mL), washed with water (10 mL) and brine (10 mL), dried over sodium sulfate, and concentrated to afford (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(1-(3-hydroxy-2,2-dimethylpropyl)-2-methyl-1H-benzo[d]imidazol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoyl)hexahydropyridazine-3-carboxylic acid (400 mg) as a gray foam, which was used directly for the next step without further purification. ESI-MS m/z=766.4 [M+H].sup.+.

[1142] Step C

[1143] To a solution of crude (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(1-(3-hydroxy-2,2-dimethylpropyl)-2-methyl-1H-benzo[d]imidazol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoyl)hexahydropyridazine-3-carboxylic acid (360 mg, 1.0 equiv) in toluene (55 mL) was added 1,2-di(pyridin-2-yl)disulfane (610 mg, 2.76 mmol, 6.0 equiv) and triphenylphosphine (610 mg, 2.32 mmol, 5.0 equiv). The solution was stirred at 85 C. for 3 hours. After concentration, the crude product was purified by silica gel chromatography (100% ethyl acetate) to give tert-butyl ((6.sup.3S,4S)-1.sup.2,10,10-trimethyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.36.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (250 mg, 64% yield) as a white foam. ESI-MS m/z=748.4 [M+H].sup.+; .sup.1H NMR (400 MHz, MeOD) 7.80 (d, J=11.8 Hz, 1H), 7.60 (q, J=6.5 Hz, 1H), 7.53 (d, J=8.5 Hz, 1H), 7.28 (s, 1H), 7.08 (s, 1H), 6.86 (s, 1H), 5.24 (d, J=3.7 Hz, 1H), 4.51-4.36 (m, 2H), 4.08 (d, J=7.1 Hz, 1H), 3.86 (d, J=11.8 Hz, 1H), 3.74 (d, J=10.8 Hz, 1H), 3.49 (d, J=11.9 Hz, 1H), 2.91-2.69 (m, 3H), 2.65 (d, J=4.1 Hz, 3H), 2.17 (d, J=10.3 Hz, 1H), 1.92 (d, J=12.6 Hz, 1H), 1.78-1.54 (m, 2H), 1.41 (d, J=16.5 Hz, 9H), 1.31 (d, J=7.2 Hz, 3H), 1.20-1.11 (m, 24H).

[1144] Step D

[1145] To a solution of tert-butyl ((6.sup.3S,4S)-1.sup.2,10,10-trimethyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (230 mg, 0.31 mmol, 1.0 equiv) in THF (5 mL) was added TBAF (1 M in THF, 0.31 mmol, 1.0 equiv). The solution was stirred at room temperature for 0.5 hours. After concentration, the crude product was diluted with ethyl acetate (20 mL) and washed with water (5 mL5). The organics were dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduce pressure to give the tert-butyl ((6.sup.3S,4S)-2.sup.5-hydroxy-1.sup.2,10,10-trimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (220 mg) as a white solid. ESI-MS m/z=592.3[M+H].sup.+.

[1146] Step E

[1147] To a solution of tert-butyl ((6.sup.3S,4S)-2.sup.5-hydroxy-1.sup.2,10,10-trimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (200 mg, 0.34 mmol, 1.0 equiv) in dichloromethane (10 mL) was added trifluoroacetic acid (2.0 mL). The resulting solution was stirred for 2 hours and then the solvent was removed under reduced pressure to give the TFA salt of (6.sup.3S,4S)-4-amino-2.sup.5-hydroxy-1.sup.2,10,10-trimethyl-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-5,7-dione (200 mg) as a white solid that was used without further purification. ESI-MS m/z=492.1 [M+H].sup.+.

[1148] Step F

[1149] To a stirred solution of (S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-3-methylbutanoic acid (48 mg, 0.20 mmol, 1.0 equiv) in DMF (6 mL) was added HATU (114 mg, 0.30 mmol, 1.5 equiv) and diethylisopropylamine (130 mg, 1.0 mmol, 5.0 equiv). After stirring for 10 minutes at room temperature, the TFA salt of (6.sup.3S,4S)-4-amino-2.sup.5-hydroxy-1.sup.2,10,10-trimethyl-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-5,7-dione (120 mg, 0.20 mmol, 1.0 equiv) in DMF (1 mL) was added. The resulting solution was stirred for 1 hour and then diluted with ethyl acetate (10 mL) and water (10 mL). The layers was separated and the organic layer was washed with water (310 mL), brine (10 mL), dried over anhydrous sodium sulfate, filtered, and the solvent was removed under reduced pressure. The residue was purified by reverse phase preparative HPLC (0.1% formic acid in MeCN/Water) to give (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.sup.3S,4S)-2.sup.5-hydroxy-1.sup.2,10,10-trimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)-3-methylbutanamide (15.5 mg, 11% yield) as a white solid.

[1150] The synthesis of Compound 1 is a representative example of the use of appropriate intermediates of Example 1 and procedures similar those described in Method A to make compounds of the invention.

Example 3Synthesis of (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.SUP.3.S,4S)-2.SUP.5.-hydroxy-6,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-8,11-dioxa-1(6,4)-quinolina-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)-3-methylbutanamide (Compound 2)

[1151] ##STR00393##

[1152] Step A

[1153] A solution of methyl (3S)-1-[(2S)-2-[[(tert-butoxy)carbonyl]amino]-3-[3-(tetramethyl-1,3,2-dioxaborolan-2-yl)-5-[[tris(propan-2-yl)silyl]oxy]phenyl]propanoyl]-1,2-diazinane-3-carboxylate (500 mg, 0.72 mmol, 1 equiv) in 1,4-dioxane (5 mL) was treated with 2-[(6-bromoquinolin-4-yl)oxy]ethyl acetate (246 mg, 0.79 mmol, 1.1 equiv), K.sub.2CO.sub.3 (1.31 g, 9.50 mmol, 13.1 equiv) and Pd(dppf)Cl.sub.2 (52.6 mg, 0.07 mmol, 0.1 equiv). The resulting solution was stirred for 6 hours at 65 C. The solids were filtered off and the solvent was removed in vacuo. The resulting residue was purified by silica gel chromatography (ethyl acetate/petroleum ether (3:1)) and purified to give methyl (3S)-1-[(2S)-3-(3-[4-[2-(acetyloxy)ethoxy]quinolin-6-yl]-5-[[tris(propan-2-yl)silyl]oxy]phenyl)-2-[[(tert-butoxy)carbonyl]amino]propanoyl]-1,2-diazinane-3-carboxylate (400 mg, 69% yield) as a yellow solid. ESI-MS m/z=793.4 [M+H].sup.+.

[1154] Step B

[1155] A solution of methyl (3S)-1-[(2S)-3-(3-[4-[2-(acetyloxy)ethoxy]quinolin-6-yl]-5-[[tris(propan-2-yl)silyl]oxy]phenyl)-2-[[(tert-butoxy)carbonyl]amino] propanoyl]-1,2-diazinane-3-carboxylate (400 mg, 0.50 mmol, 1 equiv) in DCE (4 mL) was treated with Me.sub.3SnOH (551 mg, 3.03 mmol, 6.0 equiv). The resulting solution was stirred overnight at 80 C., and then the mixture was concentrated. The solution was diluted with 100 mL of ethyl acetate and then washed with 3100 ml of 0.01 N aqueous KHSO.sub.4 followed by 100 mL of brine. The organics were dried over anhydrous sodium sulfate and filtered to give (3S)-1-[(2S)-2-[[(tert-butoxy)carbonyl]amino]-3-[3-[4-(2-hydroxyethoxy)quinolin-6-yl]-5-[[tris(propan-2-yl)silyl]oxy]phenyl]propanoyl]-1,2-diazinane-3-carboxylic acid (370 mg 99% yield) as a yellow solid which was converted to (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.sup.3S,4S)-2.sup.5-hydroxy-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-8,11-dioxa-1(6,4)-quinolina-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)-3-methylbutanamide using the appropriate intermediates of Example 1 and procedures similar those described in Method A. The synthesis of Compound 2 is a representative example of the use of Method B to make compounds of the invention.

Example 4Synthesis of 1-(2-chloroacetyl)-N-((2S)-1-(((6.SUP.3.S,4S)-1.SUP.3.-cyano-2.SUP.5.-hydroxy-10,10-dimethyl-8,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-aza-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide (Compound 3)

[1156] ##STR00394##

[1157] Step A

[1158] HATU (684 mg, 1.8 mmol, 1.2 equiv) was added to the solution of (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propanoyl)hexahydropyridazine-3-carboxylic acid (980 mg, 1.5 mmol, 1.0 equiv), 1-(3-amino-2,2-dimethylpropyl)-6-bromo-1H-indole-3-carbonitrile (549 mg, 1.8 mmol, 1.2 equiv) and DIPEA (580 mg, 4.5 mmol, 3.0 equiv) in DMF (20 mL) at 0 C. The mixture was stirred at 0 C. to 5 C. for 1 hour and then diluted with ethyl acetate (200 mL) and washed with water (150 mL2) and brine (150 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue that was purified by silica gel chromatography (ethyl acetate/petroleum ether (2:1)) to give tert-butyl ((S)-1-((S)-3-((3-(6-bromo-3-cyano-1H-indol-1-yl)-2,2-dimethylpropyl)carbamoyl)tetrahydropyridazin-1(2H)-yl)-1-oxo-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propan-2-yl)carbamate (530 mg, 36% yield) as an off-white solid. ESI-MS m/z=963.3 [M+H].sup.+.

[1159] Step B

[1160] A mixture of tert-butyl ((S)-1-((S)-3-((3-(6-bromo-3-cyano-1H-indol-1-yl)-2,2-dimethylpropyl)carbamoyl)tetrahydropyridazin-1(2H)-yl)-1-oxo-3-(3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-5-((triisopropylsilyl)oxy)phenyl)propan-2-yl)carbamate (530 mg, 0.55 mmol, 1.0 equiv), K.sub.2CO.sub.3 (190 mg, 1.375 mmol, 2.5 equiv) and Pd(dppf)Cl.sub.2.CH.sub.2Cl.sub.2 (45 mg, 0.055 mmol, 0.1 equiv) in dioxane (20 mL) and H.sub.2O (4 mL) was stirred at 80 C. for 2 hours. The mixture was diluted with ethyl acetate (100 mL) and then washed with water (50 mL2) and brine (80 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was purified by chromatography (ethyl acetate) to give tert-butyl ((6.sup.3S,4S)-1.sup.3-cyano-10,10-dimethyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-aza-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (220 mg, 53% yield) as a light yellow solid. ESI-MS m/z=757.5 [M+H].sup.+.

[1161] 1-(2-Chloroacetyl)-N-((2S)-1-(((6.sup.3S,4S)-1.sup.3-cyano-2.sup.5-hydroxy-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-aza-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide was synthesized from tert-butyl ((6.sup.3S,4S)-1.sup.3-cyano-10,10-dimethyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.36.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-aza-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate using the appropriate intermediates of Example 1 and procedures similar those described in Method A. The synthesis of Compound 3 is a representative example of the use of Method C to make compounds of the invention.

Example 5Synthesis of (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.SUP.3.S,4S)-12-ethyl-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(3,5)-pyridinacycloundecaphane-4-yl)-3-methylbutanamide (Compound 4)

[1162] ##STR00395## ##STR00396##

[1163] Step A

[1164] A solution of methyl (S)-1-((S)-3-(5-bromopyridin-3-yl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate (1.41 g, 3.0 mmol, 1.0 equiv), Pd(dppf)Cl.sub.2 (245 mg, 0.3 mmol, 0.1 eq), 3-(2-ethyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-benzo[d]imidazol-1-yl)propan-1-ol (990 mg, 3.0 mmol, 1.0 eq) and K.sub.2CO.sub.3 (1.24 g, 9.0 mmol, 3.0 eq) in dioxane (30 mL) and water (50 mL) was stirred at 90 C. for 5 hours. The reaction was quenched by addition of ice water (100 mL) and extracted with ethyl acetate (3100 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure to give a crude product, which was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH=20:1) to give methyl (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(5-(2-ethyl-1-(3-hydroxypropyl)-1H-benzo[d]imidazol-6-yl)pyridin-3-yl)propanoyl)hexahydropyridazine-3-carboxylate (940 mg, 53% yield) as a yellow solid. ESI-MS m/z=595.3 [M+H].sup.+.

[1165] Step B

[1166] To a solution of methyl (S)-1-((S)-2-((tert-butoxycarbonyl)amino)-3-(5-(2-ethyl-1-(3-hydroxypropyl)-1H-benzo[d]imidazol-6-yl)pyridin-3-yl)propanoyl)hexahydropyridazine-3-carboxylate (594 mg, 1.0 mmol, 1.0 equiv) in MeOH (10 mL) was added LiOH (120 mg, 5.0 mmol, 5.0 equiv) in H.sub.2O (2 mL) at 0 C. The mixture was stirred at 0 C. for 2 hours. The mixture was acidified to about pH 5 with 1 M HCl and extracted with ethyl acetate (100 mL2). The organic phase was washed with brine (100 mL3), dried over anhydrous sodium sulfate, filtered, and concentrated to give a residue. The crude product was used in the next step directly without further purification (580 mg crude). ESI-MS m/z=582.3 [M+H].sup.+.

[1167] Step C

[1168] A stirred solution of (S)-1-((S)-3-(3-(((benzyloxy)carbonyl)amino)-5-(1-(3-hydroxypropyl)-1H-benzo[d]imidazol-6-yl)phenyl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylic acid (580 mg, 1.0 mmol, 1.0 equiv), PySSPy (2.2 g, 10.0 mmol, 10.0 equiv), PPh.sub.3 (2.62 g, 10.0 mmol, 10.0 equiv) in toluene (120 mL) was stirred at 80 C. for 15 hours. The reaction was quenched by the addition of ice water (100 mL) and was extracted with ethyl acetate (3100 mL). The combined organic layer was dried over anhydrous sodium sulfate and concentrated under reduced pressure to give a crude product that was purified by silica gel chromatography (dichloromethane to dichloromethane/MeOH (20:1)) to give tert-butyl ((6.sup.3S,4S)-12-ethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(3,5)-pyridinacycloundecaphane-4-yl)carbamate (260 mg, 46% yield) as a yellow solid. ESI-MS m/z=564.3 [M+H].sup.+.

[1169] Step D

[1170] To a solution of tert-butyl ((6.sup.3S,4S)-12-ethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(3,5)-pyridinacycloundecaphane-4-yl)carbamate (56 mg, 0.1 mmol, 1.0 eq) in dichloromethane (30 mL) was added TFA (1 mL) dropwise at 0 C. The mixture was stirred at 0 C. for 2 hours. The mixture was concentrated to give the crude product (6.sup.3S,4S)-4-amino-12-ethyl-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(3,5)-pyridinacycloundecaphane-5,7-dione (46.3 mg) as a yellow oil that was used in the next step without further purification. ESI-MS m/z=463.3 [M+H].sup.+.

[1171] Step E

[1172] A solution of (6.sup.3S,4S)-4-amino-12-ethyl-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(3,5)-pyridinacycloundecaphane-5,7-dione (46 mg, 0.1 mmol, 1.0 eq), (S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-3-methylbutanoic acid (24.1 g, 0.1 mmol, 1.0 eq), HATU (41.8 mg, 0.11 mmol, 1.1 eq), and DIEA (64.5 mg, 0.5 mmol, 5.0 eq) in DMF (5 mL) was stirred at 0 C. for 2 hours. The mixture was poured into water and extracted with ethyl acetate (20 mL) and washed with brine (20 mL2). The organic layer was dried over anhydrous sodium sulfate and concentrated to give a residue that was purified by prep-HPLC (formic acid in MeCN/Water) to afford (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.sup.3S,4S)-12-ethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(3,5)-pyridinacycloundecaphane-4-yl)-3-methylbutanamide (6.2 mg, 9.0% yield) as a white solid. The synthesis of Compound 4 is a representative example of the use of Method D to make compounds of the invention.

Example 6Synthesis of 1-(2-chloroacetyl)-N-(2S)-1-(((6.SUP.3.S,4S,10S)-1.SUP.3.-(2-cyanophenyl)-2.SUP.5.-hydroxy-10-methyl-6,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-4-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide (Compound 5)

[1173] ##STR00397## ##STR00398##

[1174] Step A

[1175] To a stirred solution of tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-bromo-10-methyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (250 mg, 0.31 mmol, 1.0 equiv) and 2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzonitrile (215.34 mg, 0.94 mmol, 3.0 equiv) in dioxane (2.5 mL) and H.sub.2O (0.5 mL) was added Pd(DTBPf)Cl.sub.2 (41 mg, 0.063 mmol, 0.2 equiv) and K.sub.2CO.sub.3 (108 mg, 0.783 mmol, 2.5 equiv) portionwise. After stirring for 4 hours at 80 C., the solution was concentrated and the residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (5:1) to afford tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-(2-cyanophenyl)-10-methyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (200 mg, 77% yield) as a white solid. ESI-MS m/z=820.4 [M+H].sup.+.

[1176] Step B

[1177] To a solution of tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-(2-cyanophenyl)-10-methyl-5,7-dioxo-2.sup.5-((triisopropylsilyl)oxy)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (200 mg, 0.244 mmol, 1.0 equiv) in THF (2.0 mL) at 0 C. was added TBAF (64 mg, 0.244 mmol, 1.00 equiv, 1 M in THF). After stirring for 1 hour, the solution was concentrated and the residue was purified by silica gel column chromatography, eluting with petroleum ether/ethyl acetate (4:1) to afford tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-(2-cyanophenyl)-2.sup.5-hydroxy-10-methyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (190 mg, 94% yield) as an off-white solid. ESI-MS m/z=664.3 [M+H].sup.+.

[1178] Step C

[1179] To a stirred solution of tert-butyl ((6.sup.3S,4S,10S)-1.sup.3-(2-cyanophenyl)-2.sup.5-hydroxy-10-methyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate (190 mg, 0.29 mmol, 1.0 equiv) in dichloromethane (2.0 mL) at 0 C. was added TFA (1.00 mL). After stirring for 3 hours, the solution was concentrated and the residue was purified by silica gel chromatography, eluting with petroleum ether/ethyl acetate (4:1) afford 2-((6.sup.3S,4S,10S)-4-amino-2.sup.5-hydroxy-10-methyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)- indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-1.sup.3-yl)benzonitrile (150 mg, 84%) as a white solid. ESI-MS m/z=564.2 [M+H].sup.+.

[1180] Step D

[1181] To a stirred solution of 2-((6.sup.3S,4S,10S)-4-amino-2.sup.5-hydroxy-10-methyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-1.sup.3-yl)benzonitrile (60 mg, 0.11 mmol, 1.0 equiv) and (2S)-2-[1-[1-(2-chloroacetyl)azetidin-3-yl]-N-methylformamido]-3-methylbutanoic acid (34 mg, 0.12 mmol, 1.1 equiv) in DMF (1 mL) was added DIEA (27 mg, 0.213 mmol, 2 equiv) and COMU (68 mg, 0.160 mmol, 1.5 equiv) portionwise at 0 C. After stirring for 2 hours, the solution was concentrated and the resulting residue was purified by reverse phase chromatography (10 to 50% MeCN in water) to afford 1-(2-chloroacetyl)-N-((2S)-1-(((6.sup.3S,4S,10S)-1.sup.3-(2-cyanophenyl)-2.sup.5-hydroxy-10-methyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide (9.5 mg, 11% yield) as a white solid. The synthesis of Compound 5 is a representative example of the use of Method E to make compounds of the invention.

Example 7Synthesis of (2S)-2-(3-acryloyl-2-oxoimidazolidin-1-yl)-N-((6.SUP.3.S,4S)-2.SUP.5.-hydroxy-6,7-dioxo-1.SUP.3.-(tetrahydro-2H-pyran-4-yl)-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)-3-methylbutanamide (Compound 229)

[1182] ##STR00399##

[1183] (2S)-2-(3-Acryloyl-2-oxoimidazolidin-1-yl)-N-((6.sup.3S,4S)-2.sup.5-hydroxy-5,7-dioxo-1.sup.3-(tetrahydro-2H-pyran-4-yl)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)-3-methylbutanamide was synthesized from methyl (S)-1-((S)-3-(3-(1-(3-acetoxypropyl)-3-(tetrahydro-2H-pyran-4-yl)-1H-indol-6-yl)-5-((triisopropylsilyl)oxy)phenyl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate using procedures similar to those described in Method B.

Example 8Synthesis of N-((2S)-1-(((6.SUP.3.S,4S)-2.SUP.5.-amino-1.SUP.3.-cyano-10,10-dimethyl-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-1-(2-chloroacetyl)-N-methylazetidine-3-carboxamide (Compound 230)

[1184] ##STR00400##

[1185] Step A

[1186] Benzyl tert-butyl ((6.sup.3S,4S)-1-cyano-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-25,4-diyl)dicarbamate was synthesized from 1-(3-hydroxy-2,2-dimethylpropyl)-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indole-3-carbonitrile and methyl 1-(3-(3-(((benzyloxy)carbonyl)amino)-5-bromophenyl)-2-((tert-butoxycarbonyl)amino)propanoyl)hexahydropyridazine-3-carboxylate using the appropriate intermediates of Example 1 and procedures similar those described in Method A.

[1187] TFA (0.3 mL) was added to a solution of benzyl tert-butyl ((6.sup.3S,4S)-1.sup.3-cyano-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-2.sup.5,4-diyl)dicarbamate (100 mg, 0.14 mmol, 1.0 equiv) in dichloromethane (1.5 mL) at 0 C., and then stirred for 2 hours at 0 C. The mixture was concentrated to give crude ((6.sup.3S,4S)-4-amino-1.sup.3-cyano-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-2.sup.5-yl)carbamate and the residue was used to next step without further purification. ESI-MS m/z=635.3 [M+H].sup.+

[1188] Step B

[1189] HATU (53 mg, 0.14 mmol, 1.0 equiv) was added to a solution of ((6.sup.3S,4S)-4-amino-1.sup.3-cyano-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)- benzenacycloundecaphane-2.sup.5-yl)carbamate (89 mg, 0.14 mmol, 1.0 equiv), N-(1-(2-chloroacetyl)azetidine-3-carbonyl)-N-methyl-L-valine (41 mg, 0.14 mmol, 1.0 equiv) and DIPEA (54 mg, 0.42 mmol, 3.0 equiv) in DMF (2 mL) at 0 C. The mixture was maintained at that temperature for 1 hour. The mixture was diluted with ethyl acetate (20 mL) and then washed with water (15 mL2) and brine (10 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a residue. The residue was purified by prep-TLC (dichloromethane/MeOH=20/1) to give benzyl ((6.sup.3S,4S)-4-((S)-2-(1-(2-chloroacetyl)-N-methylazetidine-3-carboxamido)-3-methylbutanamido)-1.sup.3-cyano-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-2.sup.5-yl)carbamate (85.0 mg, 67% yield) as an off-white solid. ESI-MS m/z=907.1 [M+H].sup.+

[1190] Step C

[1191] Benzyl ((6.sup.3S,4S)-4-((S)-2-(1-(2-chloroacetyl)-N-methylazetidine-3-carboxamido)-3-methylbutanamido)-1.sup.3-cyano-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-2.sup.5-yl)carbamate (70 mg, 0.077 mmol, 1.0 equiv) was added to a solution of BCl.sub.3 (2 mL, 1 M in dichloromethane) at 0 C. The mixture was then stirred at room temperature for 4 hours. MeOH (2 mL) was added and the reaction solution was diluted with ethyl acetate (20 mL) and washed with water (15 mL2) and brine (10 mL). The organic phase was collected, dried over sodium sulfate, filtered and concentrated to give a crude residue. The residue was purified by prep-TLC (EA/MeOH=8/1) to give N-((2S)-1-(((6.sup.3S,4S)-2.sup.5-amino-1.sup.3-cyano-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-1-(2-chloroacetyl)-N-methylazetidine-3-carboxamide (13.2 mg, 10% yield) as an off-white solid.

Example 9Synthesis of 1-(2-chloroacetyl)-N-((2S)-1-(((6.SUP.3.S,4S)-1.SUP.3.-cyano-2.SUP.5.-methoxy-10,10-dimethyl-6,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide (Compound 231)

[1192] ##STR00401##

[1193] The title compound was synthesized from tert-butyl ((6.sup.3S,4S)-1.sup.3-cyano-2.sup.5-methoxy-10,10-dimethyl-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate using the appropriate intermediates of Example 1 and procedures similar those described in Method A.

Example 101-acryloyl-N-((2S)-1-(((6.SUP.3.S,4S)-2.SUP.5.-hydroxy-10,10-dimethyl-1.SUP.3.-(1-methylpiperidin-4-yl)-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide (Compound 232))

[1194] ##STR00402##

[1195] (6.sup.3S,4S)-4-amino-2.sup.5-hydroxy-10,10-dimethyl-1.sup.3-(1-methylpiperidin-4-yl)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-5,7-dione was synthesized from tert-butyl ((6.sup.3S,4S)-2.sup.5-hydroxy-10,10-dimethyl-1.sup.3-(1-methylpiperidin-4-yl)-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-8(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate using the appropriate intermediates of Example 1 and procedures similar those described in Method A.

[1196] The title compound was synthesized from (6.sup.3S,4S)-4-amino-2.sup.5-hydroxy-10,10-dimethyl-1.sup.3-(1-methylpiperidin-4-yl)-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane- 5,7-dione according to a procedure similar to the one described in Method A.

Example 111-(2-chloroacetyl)-N-((28)-1-(((6.SUP.3.S,4S)-1.SUP.3.-ethynyl-2.SUP.5.-hydroxy-10,10-dimethyl-5,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-4-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide (Compound 233)

[1197] ##STR00403##

[1198] (6.sup.3S,4S)-4-amino-1.sup.3-ethynyl-2.sup.5-hydroxy-10,10-dimethyl-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-5,7-dione was synthesized from tert-butyl ((6.sup.3S,4S)-1.sup.3-ethynyl-2.sup.5-hydroxy-10,10-dimethyl-5,7-dioxo-8.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)carbamate using a procedure similar to the one described in Method A.

[1199] The title compound was synthesized from (6.sup.3S,4S)-4-amino-1.sup.3-ethynyl-2.sup.5-hydroxy-10,10-dimethyl-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-indazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-5,7-dione according to a procedure similar to the one described in Method A.

Example 12Synthesis of 1-(2-chloroacetyl)-N-((2S)-1-(((6.SUP.3.S,4S)-2.SUP.5.-hydroxy-8,7-dioxo-6.SUP.1.,6.SUP.2.,6.SUP.3.,6.SUP.4.,6.SUP.5.,6.SUP.6.-hexahydro-1.SUP.1.H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide (Compound 234)

[1200] ##STR00404##

[1201] Step A

[1202] (6.sup.3S,4S)-4-amino-2.sup.5-hydroxy-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-5,7-dione was synthesized from 3-(6-bromo-1H-benzo[d]imidazol-1-yl)propan-1-ol using the appropriate intermediates of Example 1 and procedures similar those described in Method A.

[1203] To a stirred solution of (6.sup.3S,4S)-4-amino-2.sup.5-hydroxy-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-5,7-dione (45 mg, 0.10 mmol, 1.0 equiv), 1-(((9H-fluoren-9-yl)methoxy)carbonyl)azetidine-3-carboxylic acid (43.7 mg, 0.10 mmol, 1.0 equiv) and DIEA (65 mg, 0.50 mmol, 5 equiv) in DMF (2 mL) was added COMU (64 mg, 0.15 mmol, 1.5 equiv) at 0 C. The resulting mixture was stirred for 2h at 0 C. After aqueous workup the residue was purified by Prep-TLC (petroleum ether/ethyl acetate (1:1)) to afford (9H-fluoren-9-yl)methyl 3-(((2S)-1-(((6.sup.3S,4S)-2.sup.5-hydroxy-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)- pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)(methyl)carbamoyl)azetidine-1-carboxylate (60 mg, 69% yield) as a yellow oil. ESI-MS m/z=868.3 [M+H].sup.+

[1204] Step B

[1205] To a stirred solution of (9H-fluoren-9-yl)methyl 3-(((2S)-1-(((6.sup.3S,4S)-2.sup.5-hydroxy-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)(methyl)carbamoyl)azetidine-1-carboxylate (35 mg, 0.04 mmol, 1 equiv) in MeCN (0.3 mL) was added TEA (0.3 mL) in portions at 0 C. The resulting mixture was stirred for 2 hours at room temperature and then HATU (18.4 mg, 0.05 mmol, 1.20 equiv) and 2-chloroacetic acid (4.6 mg, 0.05 mmol, 1.21 equiv) was added in portions at 0 C. The resulting mixture was stirred for 2 hours at 0 C. The solvent was removed in vacuo and the crude product was purified by Prep-HPLC with the following conditions (5% to 39% MeCN in water with 0.1% FA in 10.5 min)) to afford 1-(2-chloroacetyl)-N-((2S)-1-(((6.sup.3S,4S)-2.sup.5-hydroxy-5,7-dioxo-6.sup.1,6.sup.2,6.sup.3,6.sup.4,6.sup.5,6.sup.6-hexahydro-1.sup.1H-8-oxa-1(6,1)-benzo[d]imidazola-6(1,3)-pyridazina-2(1,3)-benzenacycloundecaphane-4-yl)amino)-3-methyl-1-oxobutan-2-yl)-N-methylazetidine-3-carboxamide (3.8 mg, 13% yield) as a white solid.

Example 13Determination of CYPA-Compound Binary Complex Formation by SPR

[1206] In order to determine the affinity of compounds of the invention for cyclophilin A (CYPA), we employed surface plasmon resonance binding analysis using the following reagents, instruments and protocol as supplied by the instrument manufacturer.

[1207] Reagents and Instrument

[1208] 1. Instrument: Biacore S200 (GE Healthcare Life Sciences)

[1209] 2. Running buffer 1HBS-P+ (1HBS supplemented with 0.05% Tween20), pH7.4, and 2% DMSO.

[1210] 3. Ligand: CypA-Avi

[1211] 4. Analyte: Serial dilution of WDB compounds from 50 M to 0 M, 2-fold dilution, 10 points (10 mM stock concentration).

[1212] 5. Sensor kit: Biotin CAPture Kit (BR28-9202-34, GE Healthcare Life Sciences)

[1213] 6. Regeneration buffer 1 volume of 1 M NaOH and 3 volumes of 8 M guanidine hydrochloride (supplied with CAPture Kit, BR28-9202-34, GE Healthcare Life Sciences)

[1214] Experimental Procedure

[1215] 1. Capture of CAPreagent: 2 l/min for 60 seconds.

[1216] 2. Immobilization of ligand CypA: Use Fc1 as the reference cell; immobilize CypA at 4 g/ml using 5 l/min flowrate on Fc2 (60s), Fc3 (80s) and Fc4 (80s), respectively.

[1217] 3. Binding of analytes: Inject WDB compound dilutions sequentially at 50 l/min for association of 60 seconds. And then let it dissociates in buffer at 50 l/min for 60 seconds.

[1218] 4. Regeneration: 2 injections of regeneration buffer at 30 l/min for 60 seconds to regenerate the chip surface.

[1219] 5. Solvent correction: Run solvent correction at beginning and the end of the run. If run more than 6 compounds, add additional solvent correction every 6 compounds.

[1220] 6. Data fitting with built-in Biacore Evaluation Software: Steady-state fitting.

Example 14Determination of KRAS-BRAF Complex Disruption Through Competition with CYPA-Compound by TR-FRET

[1221] In this example, TR-FRET was used to measure the compound-facilitated disruption of a KRAS-BRAF complex. A mixture of tagless Cyclophilin A, His6-KRAS G12C-GMP-PNP, and GST-BRAF RAS binding domain was added to a 384-well assay plate containing compounds of the invention and incubated for 3 hours. A mixture of Anti-His Eu-W1024 and anti-GST allophycocyanin was then added and the reaction was incubated for an additional 1.5 hours. TR-FRET signal was read on an EnVision microplate reader (Perkin Elmer, Ex 320 nm, Em 665/615 nm). Compounds that facilitate disruption of a KRAS_BRAF complex were identified as those eliciting a decrease in the TR-FRET ratio relative to DMSO control wells. The results are showing in Table 4, below. To determine the presenter-dependence of the compound-mediated KRAS-BRAF complex disruption, Cyclophilin A was left out of the initial incubation (see Table 5). To determine G12C specificity of the compound-mediated KRAS-BRAF complex disruption, wild-type KRAS was used instead of G12C KRAS (see Table 5).

[1222] Reagents and Instrument [1223] Tagless CYPA; 519 M in PBS buffer, pH 7.4 [1224] GST BRAF; 110 M in PBS buffer, pH 7.4 [1225] His6-KRAS.sub.G12C-GMP-PNP; 50 M in PBS buffer, pH 7.4 [1226] His6-KRAS.sub.WT-GMP-PNP; 40 M in PBS buffer, pH 7.4 [1227] Anti-His Eu-W1024 (LANCE Eu-W1024; Perkin Elmer) [1228] Anti-GST allophycocyanin (Anti-GST IgG conjugated to SureLight-Allophycocyanin; Perkin Elmer; Product Number AD0059G) [1229] Test compounds, 10 mM in 100% DMSO [1230] EnVision (Perkin Elmer) [1231] Combi MultiDrop liquid dispenser with 8-channel small volume cassette [1232] 384-well ProxiPlate (black)

[1233] Experimental Protocol

[1234] 1. Use Mosquito to dispense 100 nL/well of compounds (varying concentration in DMSO-d.sub.6) into 384-well black ProxiPlate to make assay-ready-plate (ARP).

[1235] 2. Make assay buffer containing 25 mM Hepes pH 7.3, 100 mM NaCl, 5 mM MgCl.sub.2, 0.05% BSA, and 0.002% Tween-20.

[1236] 3. Make PRE-MIX A: delivers final concentrations of 50 nM of His6-KRas G12C-GTP (1-169) and 500 nM of tagless CypA (1-165) in assay buffer.

[1237] a. For presenter dependence experiments, omit addition of tagless CypA

[1238] b. For G12C/wt specificity experiments, substitute His6-KRas WT-GTP (1-169) for His6-KRas G12C-GTP (1-169)

[1239] 4. Use MultiDrop Combi to dispense PRE-MIX A into ARP, 7 l/well. Incubate 3 hr at room temperature.

[1240] 5. Make PRE-MIX B: delivers final concentrations of 10 nM of anti-His Eu-W1024 and 50 nM of anti-GST APC.

[1241] 6. Use MultiDrop Combi to dispense PRE-MIX B into ARP, 3 l/well. Shake briefly on Combi and incubate 1.5 hr at room temperature.

[1242] 7. Read on EnVision (Ex: 320 nm; Em1: 615 nm; Em2: 665 nm).

[1243] 8. Data is processed using Dotmatics. Curves are fit using a 4-parameter non-linear fit to determine the EC50 value for formation of the ternary complex.

Example 158Determination of pERK Inhibition in H358 Cells

[1244] H358 cells (5500 cells) derived from a human lung cancer were plated in a 96 well plate in media (100 uL, RPMI with 10% FBS). After 24 hours, cells were treated for 4 hours with compound or DMSO. Cells were washed twice with room temperature TBS (200 uL) and fixed for 20 minutes with 4% paraformaldehyde diluted with TBS (150 uL). Cells were washed four times for five minutes with 0.1% TritonX/TBS (150 uL) to permeabilize the membrane. Cells were incubated with TBS blocking buffer (100 uL) at room temperature for 60 minutes. Primary antibody (Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb #4370, Cell Signaling Technology; 1:200) was added, and the cells were incubated overnight at 4 C. The cells were washed four times for five minutes with 0.1% Tween 20/TBS (150 uL). Secondary antibody (IRDye 800CW Goat-Anti-Rabbit IgG, Li-Cor Biosciences; 1:1000) and DRAQ5 (Invitrogen; 1:2000) was added, and the cells were incubated for 1 hour at room temperature. The cells were washed four times for five minutes with 0.1% Tween 20/TBS (150 uL) and scanned using the LICOR (700 and 800 nm).

[1245] The SPR CypA K.sub.D, biochemical BRAF-KRAS G12C-GTP disruption assay EC.sub.50, and cellular inhibition of pERK IC.sub.50 of compounds described herein are shown in Table 4. For CYPA Binding Affinity: A, K.sub.D5.0 M; B, 5.0 M<K.sub.D15 M; C, K.sub.D>15 M. For BRAF-GTP-KRAS-G12C Disruption: A, EC.sub.500.5 M; B, 0.5 M<EC.sub.505.0 M; C, EC.sub.50>5.0 M. For Cellular pERK Inhibition: A, IC.sub.501.0 M; B, 1.0 M<IC.sub.5010 M; C, IC.sub.50>10.0 M. Blanks in the table represent that the compound was not tested in the indicated assay.

TABLE-US-00030 TABLE 4 CYPA Binding BRAF-GTP-KRAS-G12C Cellular pERK # Affinity Disruption inhibition 1 B A C 2 A C 3 A A C 4 B C 5 A A A 6 A B 7 A A 8 A B 9 C A A 10 B B 11 A A C 12 A C 13 C C C 14 C C C 15 B A A 16 B B C 17 A B C 18 C B C 19 A A B 20 C C C 21 C B C 22 B A C 23 C A C 24 B A A 25 B A C 26 B B C 27 B A B 28 A B C 29 A C C 30 A B B 31 C B C 32 A C C 33 B A A 34 B A B 35 B C C 36 B A B 37 B A B 38 B A B 39 B A C 40 B A B 41 A A B 42 B B B 43 B A B 44 B A B 45 C C C 46 B A A 47 B A B 48 B A B 49 C A B 50 B A B 51 A B 52 B A A 53 C B C 54 C B C 55 C B C 56 C A C 57 C A B 58 C B C 59 C C C 60 B C C 61 B B B 62 B A A 63 B A A 64 B A B 65 C A B 66 C A C 67 C A B 68 A A B 69 A A B 70 A C 71 C A B 72 A A B 73 A A B 74 A A B 75 A A A 76 A A A 77 A A A 78 B A B 79 C A A 80 C A A 81 B A A 82 B A B 83 B A B 84 A C 85 A A A 86 C A A 87 B A B 88 C B 89 B B B 90 B B A 91 B B B 92 A B C 93 A C 94 A B 95 A B 96 A B 97 C A C 98 B A 99 C A A 100 C A A 101 A B 102 B A C 103 C A B 104 C A A 105 B B 106 B A B 107 A B C 108 B A C 109 C A C 110 A A B 111 B A C 112 C C C 113 A A B 114 A A B 115 A A C 116 B A B 117 B A A 118 C A B 119 C A B 120 C A C 121 A A B 122 B A A 123 C A A 124 C A A 125 C A B 126 B A C 127 B A B 128 B A A 129 B A C 130 B A C 131 B B 132 A C 133 A C 134 A B C 135 B C 136 B B 137 A C 138 C 139 B A C 140 C B 141 A B 142 A C 143 B A C 144 B C 145 B B 146 B B 147 A A C 148 C B 149 A B 150 B B 151 B A C 152 B A B 153 B C 154 B B 155 A B C 156 C A C 157 A C C 158 A C C 159 C C C 160 B B C 161 C C C 162 C C C 163 B C C 164 A C 165 A C 166 A A 167 A B 168 A A 169 A B 170 B B 171 B B 172 B B C 173 A C 174 A C 175 B A C 176 B A B 177 B A B 178 B B C 179 A A B 180 C A B 181 A 182 A C 183 B B B 184 A B C 185 A B C 186 B C C 187 B C C 188 A C C 189 A A A 190 A B C 191 B A A 192 B A B 193 B B C 194 A B C 195 B A C 196 C A B 197 A A B 198 A A C 199 B B C 200 C A C 201 C A A 202 A A B 203 C C C 204 B C B 205 C C C 206 A C C 207 C A A 208 B A B 209 B A A 210 C A A 211 B A A 212 C A B 213 C A B 214 C B 215 B A B 216 B A C 217 B A A 218 A A A 219 A A B 220 B A A 221 A A A 222 A A A 223 A A B 224 A A B 225 A A B 226 A A B 227 B C 228 A B 229 B C 230 B B C 231 B C C 232 A B C 233 C A 234 A B 235 C C 236 B A B 237 A B C 238 C A C 239 A A B 240 B A B 241 C A B 242 C A B 243 A B C 244 C B C 245 B B C 246 B A B 247 C A C 248 A B B 249 A B C 250 C A A 251 A B C 252 A B B 253 C A B 254 C C C 255 B B C 256 A A A 257 B A B 258 A C C 259 B B C 260 A C B 261 C B B 262 A A A 263 B B B 264 C A A 265 A A A 266 A A A 267 C A B 268 B A A 269 C A B 270 B A A 271 A A 272 B C C 273 C A C 274 B A A 275 C A B 276 B B C 277 B C C 278 C A B 279 A A B 280 C B C 281 B B B 282 B B C 283 B A A 284 B C C 285 B A B 286 C B C 287 B B B 288 B C C 289 B B C 290 B C C 291 A C C 292 A B C 293 B A B 294 B A A 295 C B B 296 C A B 297 C A C 298 A C C 299 B B B 300 B A A 301 B A A 302 B A B 303 A A C 304 A A B 305 C A B 306 C A B 307 B A A 308 B B B 309 A C C 310 C B 311 C C 312 B C 313 A A 314 A C 315 A C 316 B A 317 B B 318 B C 319 B C 320 A C 321 A C 322 A C 323 C 324 B A A 325 C C C 326 A C C 327 A C C 328 A B C 329 B C B 330 A A A 331 A C C 332 B C C 333 A C C 334 A C C 335 C C 336 B C 337 A B A 338 A C C 339 A C C 340 A B B 341 C C C 342 C C C 343 A B B 344 B B B 345 A A B 346 A B B 347 C C 348 B B 349 A B C 350 A B C 351 A A A 352 A A A 353 B A A 354 B B B 355 A C C 356 B B C 357 B B B 358 B B A 359 B B 360 B B 361 C A A 362 B B B 363 B C C 364 A B A 365 C B C 366 C C C 367 C B B 368 C C C 369 C B B 370 C A B 371 C C 372 B A 373 C B 374 C C 375 C C 376 A A A 377 A B A 378 A B B 379 A B A 380 C C C 381 A A A 382 B B A 383 B C 384 B B B 385 B B 386 B B A 387 B C C 388 B B B 389 B B B 390 C C 391 A C C 392 B A A 393 B B B 394 B B C 395 A B A 396 C B 397 A A A 398 A B A 399 B B B 400 B A B 401 B C 402 A A 403 C A 404 C A B 405 B B A 406 C B A 407 B B B 408 C B B 409 C B B 410 C B C 411 B B B 412 B B C 413 B A B 414 B C 415 A C 416 B B 417 C C 418 C A C

[1246] The BRAF-KRAS disruption assay results of exemplary compounds in the absence of cyclophilin A or using wild-type (WT) KRAS instead of KRAS-G12C are shown in Table 5. These results demonstrate that the tested compounds: (1) require the presence of CYPA to cause disruption of the BRAF-GTP-KRAS-G12C complex; and (2) are incapable of disrupting a BRAF-GTP-KRAS complex when wild-type KRAS is used.

TABLE-US-00031 TABLE 5 BRAF-GTP-KRAS-G12C BRAF-GTP-KRASWT Compound complex disruption complex disruption Number without CYPA (IC.sub.50, M) with CYPA (IC.sub.50, M) 110 >30 >30 169 >30 >30 192 >30 >30 221 >30 >30 226 >30 >30

Example 16Determination of Percentage of Crosslinking to KRAS-G12C in the Presence of BME Materials and Reagents

[1247] 1. 10 Incubation buffer 125 mM HEPES pH 7.4, 750 mM NaCl, 10 mM MgCl.sub.2

[1248] 2. 50 M Protein A stock (CypA) in incubation buffer

[1249] 3. 5 M Protein B (G12C-GMPNP) stock in incubation buffer

[1250] 4. 20 M compound stock in 1 Incubation buffer with 10% DMSO

[1251] 5. 25 mM BME stock solution (prepared by diluting the BME stock in Milli Q water)

[1252] Procedure:

[1253] CypA 1-165 (final concentration 5 M), test compound (final concentration 2 M) and G12C-GMPNP (final concentration 0.5 M) were incubated in the incubation buffer (125 mM HEPES pH 7.4, 750 mM NaCl, 10 mM MgCl.sub.2) for the requisite amount of time at room temperature. The samples were quenched with formic acid, final concentration 0.5%, 10 L aliquots were injected to TOF-MS.

[1254] Sequence of Addition for a Final Incubation Volume of 50 L

[1255] 1. 5 L 10 incubation buffer

[1256] 2. 28 ul H.sub.2O

[1257] 3. 2 L from 25 mM BME stock

[1258] 4. 5 L CypA at 50 uM

[1259] 5. 5 L from 5 M G12C-GMPNP

[1260] 6. 5 L compound from 20 M compound stock

[1261] TOF-MS Analysis:

[1262] LC-MS is performed on an Agilent 6230 TOF-LC mass spectrometer equipped with an electrospray probe operated in positive ionization mode, 10 L samples are injected on a Sepax Bio-C4, 300 , 2.1100 mm column. The mobile phase is 0.1% (vol/vol) formic acid and 0.1% 1 mM ammonium formate in 95% water, 4.8% acetonitrile (A) and 0.1% (vol/vol) formic acid and 0.1% 1 mM ammonium formate in 95% acetonitrile and 4.8% water (B). The separation is performed by a 9 min total gradient consisting of 5 min linear gradient from 25% to 50% B, and a wash at 100% B for 1.25 min, all at a flow rate of 0.6 mL/min (see attached timetable below). Mass spectrometer source conditions were capillary voltage, 4,000 V; cone voltage, 120 V; source temperature, 275 C.; scan range, 100-2,000 a.m.u. with a cycle time of 1 s.

[1263] Calculations:

[1264] The observed mass is generated by averaging the major peak in the total ion current (TIC). The charge-state series of the species are deconvoluted using Agilent MassHunter Bioconfirm using maximum entropy setting (range is set to 17000-23000 Da). Integration of deconvoluted protein peaks (bound and unbound species) enables % bound calculation using equation: % bound to Protein B=peak height of bound species/[peak height of bound species+peak height of unbound]100.

[1265] The biochemical crosslinking assay results of compounds described herein are shown in Table 6.

TABLE-US-00032 TABLE 6 % Crosslinking with KRAS-G12C-GTP Compound Number in presence of CYPA, 5 min incubation 110 83 169 .sup.39.sup. 192 62 221 28 226 27 .sup.60 minute incubation

[1266] These results show that the exemplary compounds are capable of cross-linking to KRAS-G12C in the presence of CYPA and therefore should form a covalent bond to the cysteine at amino acid 12 in KRAS-G12C in vivo.

OTHER EMBODIMENTS

[1267] While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure come within known or customary practice within the art to which the invention pertains and may be applied to the essential features hereinbefore set forth.

[1268] All publications, patents and patent applications are herein incorporated by reference in their entirety to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety.

[1269] The invention is also described by the enumerated items below.

[1270] 1. A compound of formula I:

##STR00405##

a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein:

[1271] Q is a bicyclic arylene, a bicyclic heteroarylene, or a bicyclic heterocyclylene, wherein a first ring in Q is bonded to X, and a second ring in Q is bonded to Z, and wherein Q is optionally substituted;

[1272] X is a bond; a straight chain C.sub.1-C.sub.3 alkylene optionally substituted with 1 to 3 substituents independently selected from fluoro, CN, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; O; S(O).sub.0-2; *CH.sub.2O; *CH.sub.2S(O).sub.0-1; *OCH.sub.2; or *CH.sub.2S(O).sub.0-2, wherein * represents a portion of X bound to C(R.sup.4)(R.sup.5);

[1273] Y is O, NH or N(C.sub.1-C.sub.3 alkyl)-;

[1274] ring Z is phenyl or a 6-membered heteroaryl;

[1275] R.sup.1 is optionally substituted C.sub.1-C.sub.6 alkyl, (CH.sub.2).sub.0-1(C.sub.3-C.sub.6 optionally substituted cycloalkyl), (CH.sub.2).sub.0-1-(optionally substituted aryl), or optionally substituted heterocyclyl;

[1276] R.sup.2 is:

##STR00406##

[1277] wherein: [1278] ring A is a 4-8 membered cycloalkyl or a 4-8 membered heterocyclyl; [1279] W is N(R.sup.12), O, or C(R.sup.12a)(R.sup.12b); [1280] each R.sup.A is each independently fluoro; chloro; CN; OH; NH.sub.2; C.sub.1-C.sub.3 alkyl optionally substituted with CN, OH, NH.sub.2 or OC.sub.1-C.sub.3 alkyl; OC.sub.1-C.sub.3 alkyl; or NHC.sub.1-C.sub.3 alkyl; [1281] R.sup.9, if present, is N(C.sub.0-C.sub.5 alkylene-H), N(C(O)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C.sub.0-C.sub.5 alkylene-H), or C(C.sub.0-C.sub.3 alkylene-H)(C(O)C.sub.0-C.sub.5 alkylene-H), wherein each alkylene portion of R.sup.9 is optionally substituted with one or more substituent, wherein each substituent is, independently, selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; [1282] R.sup.10, if present, is C.sub.1-C.sub.4 alkylene optionally substituted with one or more substituent, wherein each substituent is, independently, selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; [1283] R.sup.11 is N(C.sub.0-C.sub.5 alkylene-H), N(C(O)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C(O)C.sub.0-C.sub.5 alkylene-H), or a saturated, nitrogen-containing heterocyclyl, where each alkylene portion of R.sup.11 is optionally substituted with one or more substituent, wherein each substituent is, independently, selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; [1284] R.sup.12 is hydrogen, or C.sub.1-C.sub.3 alkyl, or [1285] R.sup.12 is taken together with one R.sup.A, the atoms to which they are respectively attached and any intervening atoms to form an optionally substituted, 5-8 membered heterocyclyl that is fused or spiro-fused to ring A, or [1286] R.sup.12 is taken together with any methylene unit in R.sup.10, or any methylene unit in R.sup.11, the atoms to which they are respectively attached and any intervening atoms to form an optionally substituted, 5-8 membered heterocyclyl; [1287] each of R.sup.12 and R.sup.12b are independently hydrogen, or C.sub.1-C.sub.3 alkyl, or R.sup.12a and R.sup.12b are taken together with the carbon atom to which they are bound to form a 3-6 membered cycloalkyl ring; [1288] R.sup.13 is O, S, NCN, or NOC.sub.1-C.sub.3 alkyl; and [1289] WH is

##STR00407## [1290] each R.sup.14 is independently hydrogen, CN, or C.sub.1-C.sub.3 alkyl optionally substituted with one or more substituents independently selected from OH, OC.sub.1-C.sub.3 alkyl, NH.sub.2, NH(C.sub.1-C.sub.3 alkyl), N(C.sub.1-C.sub.3 alkyl).sub.2, or an optionally substituted 4-7 membered saturated heterocyclyl; [1291] R.sup.15 is C.sub.1-C.sub.3 alkyl optionally substituted with one or more substituents independently selected from OH, OC.sub.1-C.sub.3 alkyl, NH.sub.2, NH(C.sub.1-C.sub.3 alkyl), N(C.sub.1-C.sub.3 alkyl).sub.2, or an optionally substituted 4-7 membered saturated heterocyclyl; [1292] R.sup.16 is hydrogen, C.sub.1-C.sub.3 alkyl optionally substituted with one or more substituents independently selected from OH, OC.sub.1-C.sub.3 alkyl, NH.sub.2, NH(C.sub.1-C.sub.3 alkyl), N(C.sub.1-C.sub.3 alkyl).sub.2, or an optionally substituted 4-7 membered saturated heterocyclyl; or [1293] R.sup.14 is taken together with either of R.sup.9 or R.sup.11, the atoms to which they are attached and any intervening atoms to form an optionally substituted 5-8 membered ring system; or [1294] R.sup.16 is taken together with either of R.sup.9 or R.sup.11, the atoms to which they are attached and any intervening atoms to form an optionally substituted 5-8 membered ring system;

[1295] R.sup.3 is hydrogen, halogen, C.sub.1-C.sub.3 alkyl, or C.sub.1-C.sub.3 hydroxyalkyl;

[1296] R.sup.4 is hydrogen, halogen, or optionally substituted C.sub.1-C.sub.3 alkyl;

[1297] R.sup.5 is hydrogen, halogen, OH, CN, O-(optionally substituted C.sub.1-C.sub.3 alkyl), optionally substituted C.sub.1-C.sub.3 alkyl, optionally substituted C.sub.2-C.sub.6 alkenyl, optionally substituted C.sub.2-C.sub.8 alkynyl, (CH.sub.2).sub.0-1-aryl, (CH.sub.2).sub.0-1-heteroaryl, (CH.sub.2).sub.0-1-cycloalkyl, or (CH.sub.2).sub.0-1-heterocyclyl; or

[1298] R.sup.4 and R.sup.5 are taken together to form CH.sub.2, an optionally substituted C.sub.3-C.sub.6 cycloalkyl, or a 3-7 membered saturated heterocyclyl; or

[1299] R.sup.5 is taken together with a ring atom in Q, the carbon atom to which R.sup.4 is bound and X to form a 4-9 membered saturated or unsaturated heterocyclyl that is fused to Q;

[1300] R.sup.6 is hydrogen or CH.sub.3;

[1301] each R.sup.7 is independently halo, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 hydroxyalkyl, OH, OC.sub.1-C.sub.3 alkyl, OC.sub.1-C.sub.3 haloalkyl, NR.sup.n1R.sup.n2, NR.sup.n1OR.sup.n2, ONR.sup.n1R.sup.n2, or NR.sup.n1NR.sup.n2R.sup.n3;

[1302] R.sup.n1 is H, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 heteroalkyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 aminoalkyl, wherein one methylene unit of R.sup.n1 is optionally substituted with

##STR00408##

[1303] R.sup.n2 is H, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 heteroalkyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 aminoalkyl, wherein one methylene unit of R.sup.n2 is optionally substituted with

##STR00409##

[1304] R.sup.n3 is H, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 heteroalkyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 aminoalkyl, wherein one methylene unit of R.sup.n3 is optionally substituted with

##STR00410##

[1305] each R.sup.6 is independently halo, C.sub.1-C.sub.3 alkyl, or C.sub.1-C.sub.3 haloalkyl;

[1306] n is 0, 1, 2, 3, 4, 5, or 6;

[1307] p is 0, 1, 2, or 3; and

[1308] r is 0, 1, 2, 3, or 4.

[1309] 2. The compound of item 1, a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Y is O.

[1310] 3. The compound of item 1, a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Y is NH.

[1311] 4. The compound of item 1, a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Y is N(C.sub.1-C.sub.3 alkyl)-.

[1312] 5. The compound of any one of items 1-4, a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein WH is:

##STR00411##

[1313] 6. The compound of any one of items 1-4, a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein WH is:

##STR00412##

[1314] 7. The compound of any one of items 1-4, a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein WH is:

##STR00413##

[1315] 8. The compound of any one of items 1-4, a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein WH is:

##STR00414##

[1316] 9. The compound of any one of items 1-4, a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein WH is:

##STR00415##

[1317] 10. The compound of any one of items 1-9, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Z is phenyl.

[1318] 11. The compound of item 10, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Z is 3-hydroxyphen-1,5-diyl.

[1319] 12. The compound of any one of items 1-9, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Z is a 6-membered heteroaryl.

[1320] 13. The compound of item 12, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Z is pyridyl.

[1321] 14. The compound of any one of items 1-13, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein n is 0.

[1322] 15. The compound of any one of items 1-13, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein n is 1.

[1323] 16. The compound of any one of items 1-13, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein n is 2.

[1324] 17. The compound of any one of items 1-13, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein n is 3.

[1325] 18. The compound of any one of items 1-13, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein n is 4.

[1326] 19. The compound of any one of items 1-13, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein n is 5.

[1327] 20. The compound of any one of items 1-13, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein n is 6.

[1328] 21. The compound of any one of items 1-20, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein p is 0.

[1329] 22. The compound of any one of items 1-20, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein p is 1.

[1330] 23. The compound of any one of items 1-20, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein p is 2.

[1331] 24. The compound of any one of items 1-20, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein p is 3.

[1332] 25. The compound of any one of items 1-24, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein r is 0.

[1333] 26. The compound of any one of items 1-24, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein r is 1.

[1334] 27. The compound of any one of items 1-24, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein r is 2.

[1335] 28. The compound of any one of items 1-24, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein r is 3.

[1336] 29. The compound of any one of items 1-24, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein r is 4.

[1337] 30. The compound of any one of items 1-29, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.3 is H.

[1338] 31. The compound of any one of items 1-29, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.3 is halogen.

[1339] 32. The compound of any one of items 1-29, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.3 is C.sub.1-C.sub.3 alkyl.

[1340] 33. The compound of any one of items 1-29, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.3 is C.sub.1-C.sub.3 hydroxyalkyl.

[1341] 34. The compound of any one of items 1-33, wherein X is CH.sub.2.

[1342] 35. The compound of any one of items 1-33, wherein X is a bond.

[1343] 36. The compound of any one of items 1-11, 14-20, 30, 34 and 35, wherein said compound has the structure of formula (Ia):

##STR00416##

or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof,

[1344] wherein:

[1345] X is a bond, O, CH.sub.2, CH(CH.sub.3), *CH.sub.2O, or CH.sub.2CH.sub.2, wherein * represents a portion of X bound to C(R.sup.4)(R.sup.5);

[1346] Y is O or NH;

[1347] R.sup.1 is C.sub.1-C.sub.4 alkyl, (CH.sub.2).sub.0-1(C.sub.3-C.sub.6 cycloalkyl), or C.sub.4-C.sub.8 cycloalkyl;

[1348] R.sup.2 is:

##STR00417##

[1349] wherein: [1350] ring A is a 4-8 membered cycloalkyl or a 4-8 membered saturated heterocyclyl; [1351] each R.sup.A is each independently fluoro; chloro; CN; OH; NH.sub.2; C.sub.1-C.sub.3 alkyl optionally substituted with CN, OH, NH.sub.2 or OC.sub.1-C.sub.3 alkyl; OC.sub.1-C.sub.3 alkyl; or NHC.sub.1-C.sub.3 alkyl; [1352] n is 0, 1, 2, 3, 4, 5, or 6; [1353] R.sup.9, if present, is N(C.sub.0-C.sub.5 alkylene-H), N(C(O)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C.sub.0-C.sub.5 alkylene-H), or C(C.sub.0-C.sub.3 alkylene-H)(C(O)C.sub.0-C.sub.5 alkylene-H), wherein each alkylene portion of R.sup.9 is optionally substituted with one or more substituent independently selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; [1354] R.sup.10, if present, is C.sub.1-C.sub.4 alkylene optionally substituted with one or more substituent independently selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; [1355] R.sup.11 is N(C.sub.0-C.sub.5 alkylene-H), N(C(O)(C.sub.0-C.sub.5 alkylene-H), C(C.sub.0-C.sub.3 alkylene-H)(C.sub.0-C.sub.5 alkylene-H), or C(C.sub.0-C.sub.3 alkylene-H)(C(O)C.sub.0-C.sub.5 alkylene-H), wherein each alkylene portion of R.sup.11 is optionally substituted with one or more substituent independently selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl; [1356] R.sup.12 is hydrogen, or C.sub.1-C.sub.3 alkyl, or [1357] R.sup.12 is taken together with one R.sup.A, the atoms to which they are respectively attached and any intervening atoms to form an optionally substituted, 5-8 membered heterocyclyl that is fused to ring A, or [1358] R.sup.12 is taken together with any methylene unit in R.sup.10, or any methylene unit in R.sup.11, the atoms to which they are respectively attached and any intervening atoms to form an optionally substituted, 5-8 membered heterocyclyl; [1359] WH is

##STR00418## [1360] each R.sup.14 is independently hydrogen, CN, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 hydroxyalkyl, OC.sub.1-C.sub.3 alkyl; [1361] R.sup.15 is C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 alkylene-OC.sub.1-C.sub.3 alkyl; [1362] R.sup.16 is hydrogen, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 hydroxyalkyl, or C.sub.1-C.sub.3 alkylene-OC.sub.1-C.sub.3 alkyl; or [1363] R.sup.14 is taken together with either of R.sup.9 or R.sup.11, the atoms to which they are attached and any intervening atoms to form an optionally substituted 5-8 membered ring system, or [1364] R.sup.16 is taken together with either of R.sup.9 or R.sup.11, the atoms to which they are attached and any intervening atoms to form an optionally substituted 5-8 membered ring system;

[1365] R.sup.4 is hydrogen, halo, or C.sub.1-C.sub.3 alkyl;

[1366] R.sup.5 is hydrogen, halo, OH, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 hydroxyalkyl, C.sub.1-C.sub.3 alkylene-OC.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 haloalkyl, (CH.sub.2).sub.0-1C.sub.3-C.sub.6 cycloalkyl, C.sub.1-C.sub.3 cyanoalkyl, or (CH.sub.2).sub.0-1-aryl (benzyl), or

[1367] R.sup.4 and R.sup.5 are taken together to form CH.sub.2, or a C.sub.3-C.sub.6 cycloalkyl, or

[1368] R.sup.5 is taken together with a ring atom of Q, the carbon atom to which it is bound and X to form a 5-7 membered saturated heterocyclyl;

[1369] R.sup.7 is OH, NH.sub.2, or C.sub.1-C.sub.3 haloalkyl;

[1370] Q is a bicyclic arylene, a bicyclic heteroarylene, or a bicyclic heterocyclylene, wherein:

[1371] a first ring in Q is bonded to X, and a second ring in Q is bonded Z; and

[1372] Q is optionally substituted with one or more independently selected substituents selected from O; CN; C.sub.1-C.sub.5 alkyl optionally substituted with one or more independently selected halo, CN, OH, O(C.sub.1-C.sub.3 alkyl), C(O)(C.sub.1-C.sub.3 alkyl), O(C.sub.2-C.sub.3 alkynyl), (C.sub.3-C.sub.6 cycloalkyl), or a 4-7 membered saturated heterocyclyl; O(C.sub.1-C.sub.3 alkyl) optionally substituted with one or more independently selected halo; C.sub.2-C.sub.5 alkenyl optionally substituted with one or more independently selected CN, or OH; C.sub.2-C.sub.3 alkynyl; S(O).sub.2C.sub.1-C.sub.3 alkyl; (CH.sub.2).sub.0-1C.sub.3-C.sub.6 cycloalkyl optionally substituted with one or more independently selected halo, O, CN, C.sub.1-C.sub.3 alkyl optionally substituted with CN or OC.sub.1-C.sub.3 alkyl, C(O)-saturated heterocyclyl, O-saturated heterocyclyl, O-cycloalkyl, or O-aryl; (CH.sub.2).sub.0-1-heteroaryl optionally substituted with one or more independently selected halo, CN, C.sub.1-C.sub.3 alkyl optionally substituted with CN or OC.sub.1-C.sub.3 alkyl, C(O)-saturated heterocyclyl, O-saturated heterocyclyl, O-cycloalkyl, or O-aryl; (CH.sub.2).sub.0-1-heterocyclyl optionally substituted with one or more independently selected halo, O, CN, C.sub.1-C.sub.3 alkyl optionally substituted with CN or OC.sub.1-C.sub.3 alkyl, C(O)-saturated heterocyclyl, O-saturated heterocyclyl, O-cycloalkyl, or O-aryl; (CH.sub.2).sub.0-1-aryl optionally substituted with one or more independently selected halo, CN, C.sub.1-C.sub.3 alkyl optionally substituted with CN or OC.sub.1-C.sub.3 alkyl, C(O)-saturated heterocyclyl, O-saturated heterocyclyl, O-cycloalkyl, or O-aryl; C(O)NH(C.sub.1-C.sub.3 alkyl); C(O)N(C.sub.1-C.sub.3 alkyl).sub.2; C.sub.2-C.sub.3 alkenylene NO(C.sub.1-C.sub.3 alkyl) optionally substituted with C.sub.3-C.sub.6 cycloalkyl; or

[1373] two substituents on the same or adjacent ring atoms of Q are taken together to form a 5-7 membered monocyclic ring or a 6-12 membered bicyclic ring optionally substituted with one or more independently selected halo, O, CN, C.sub.1-C.sub.3 alkyl, or OC.sub.1-C.sub.3 alkyl; and fused to Q.

[1374] 37. The compound of item 36, wherein said compound has the structure of formula (Ib):

##STR00419##

or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

[1375] 38. The compound of item 36, wherein said compound has the structure of formula (Ic):

##STR00420##

or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

[1376] 39. The compound of any one of items 1-38, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is a 5,6 bicyclic heteroarylene, a 5,6 bicyclic heterocyclylene, a 6,6 bicyclic heteroarylene, or a 6,6 bicyclic heterocyclylene; and wherein Q is optionally substituted.

[1377] 40. The compound of any one of items 1-38, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is a 5,6 bicyclic heteroarylene, wherein Q is optionally substituted.

[1378] 41. The compound of any one of items 1-38, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is a 5,6 bicyclic heterocyclylene, wherein Q is optionally substituted.

[1379] 42. The compound of any one of items 1-38, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is a 6,6 bicyclic heteroarylene, wherein Q is optionally substituted.

[1380] 43. The compound of any one of items 1-38, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is a 6,6 bicyclic heterocyclylene, wherein Q is optionally substituted.

[1381] 44. The compound of item 43, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of:

##STR00421##

[1382] wherein:

[1383] each of V.sub.1, V.sub.2, V.sub.3 and V.sub.4 is independently C, CH, or N;

[1384] R.sup.Q1 is S(O).sub.2R.sup.Q11, C(O)R.sup.Q11, S(O).sub.2N(R.sup.Q11)R.sup.Q12, C(O)N(R.sup.Q11)R.sup.Q12, C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl are optionally substituted; or

[1385] R.sup.Q1 is taken together with the nitrogen atom to which it is attached and an adjacent ring atom to form an optionally substituted 4-8 membered ring, which is optionally further fused to a 5-6 membered ring;

[1386] each of R.sup.Q11 and R.sup.Q12 is independently C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein each of R.sup.Q11 and R.sup.Q12 is optionally substituted; or

[1387] R.sup.Q11 and R.sup.Q12 are taken together with the nitrogen atom to which they are both attached to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking R.sup.Q11 and R.sup.Q12 together is optionally fused to another 5-6 membered ring.

[1388] 45. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00422##

[1389] 46. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00423##

[1390] 47. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00424##

[1391] 48. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00425##

[1392] 49. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00426##

[1393] 50. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00427##

[1394] 51. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00428##

[1395] 52. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00429##

[1396] 53. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00430##

[1397] 54. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00431##

[1398] 55. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00432##

[1399] 56. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00433##

[1400] 57. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00434##

[1401] 58. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00435##

[1402] 59. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00436##

[1403] 60. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00437##

[1404] 61. The compound of item 44, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00438##

[1405] 62. The compound of item 43, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of:

##STR00439##

[1406] wherein:

[1407] each of V.sub.1, V.sub.2, V.sub.3 and V.sub.4 is independently C, CH, N, C(F), C(CH.sub.3), C(OH), C(OCH.sub.3), or C(CN);

[1408] each of V.sub.5, V.sub.6, and V.sub.7 is independently, C(R.sup.17a)(R.sup.17b), or C(O), wherein each of R.sup.17a and R.sup.17b is independently selected from hydrogen, halo, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 haloalkyl, OC.sub.1-C.sub.3 alkyl, OC.sub.1-C.sub.3 haloalkyl, and no more than two of V.sub.5, V.sub.6, and V.sub.7 is C(O);

[1409] R.sup.NQ1 is hydrogen, optionally

substituted S(O).sub.2R.sup.Q11, C(O)R.sup.Q11, S(O).sub.2N(R.sup.Q11)R.sup.Q12, C(O)N(R.sup.Q11)R.sup.Q12, C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl are optionally substituted;

[1410] each R.sup.Q2 is independently hydrogen, CN, optionally

substituted S(O).sub.2R.sup.Q11, C(O)R.sup.Q11, S(O).sub.2N(R.sup.Q11)R.sup.Q12, C(O)N(R.sup.Q11)R.sup.Q12, C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl are optionally substituted; or

[1411] R.sup.NQ1 and one R.sup.Q2 are taken together with the atoms to which they are bound to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking R.sup.NQ1 and one R.sup.Q2 together is optionally further fused to a 5-6 membered ring;

[1412] each R.sup.Q3 is independently hydrogen, CN, optionally

substituted S(O).sub.2R.sup.Q11, C(O)R.sup.Q11, S(O).sub.2N(R.sup.Q11)R.sup.Q12, C(O)N(R.sup.Q11)R.sup.Q12, C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl are optionally substituted, or

[1413] two R.sup.Q3 bound to the same atom are taken together to form CH, O, S, or NR.sup.V4; or

[1414] two R.sup.Q3 bound to the same atom are taken together with the atom to which they are bound to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking each R.sup.Q3 together is optionally further fused to a 5-8 membered ring; or

[1415] R.sup.NQ1 and one R.sup.Q3 are taken together with the atoms to which they are bound to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking R.sup.NQ1 and R.sup.Q3 together is optionally further fused to a 5-6 membered ring;

[1416] each of R.sup.Q11 and R.sup.Q12 is independently C.sub.1-C.sub.10 alkyl, C.sub.3-C.sub.10 cycloalkyl, a 4-14 membered heterocyclyl, aryl, or heteroaryl, wherein each of R.sup.Q11 and R.sup.Q12 is optionally substituted; or

[1417] R.sup.Q11 and R.sup.Q12 are taken together with the atoms to which they are attached to form an optionally substituted 4-8 membered ring, wherein the ring formed by taking R.sup.Q11 and R.sup.Q12 together is optionally fused to another 5-6 membered ring; and

[1418] * represents a portion of Q that is bound to ring Z.

[1419] 63. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00440##

[1420] 64. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00441##

[1421] 65. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00442##

[1422] 66. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00443##

[1423] 67. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00444##

[1424] 68. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00445##

[1425] 69. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00446##

[1426] 70. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00447##

[1427] 71. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00448##

[1428] 72. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00449##

[1429] 73. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00450##

[1430] 74. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00451##

[1431] 75. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00452##

[1432] 76. The compound of item 62, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00453##

[1433] 77. The compound of item 43, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of:

##STR00454##

[1434] 78. The compound of item 77, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00455##

[1435] 79. The compound of item 77, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00456##

[1436] 80. The compound of item 77, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00457##

[1437] 81. The compound of item 77, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00458##

[1438] 82. The compound of item 77, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00459##

[1439] 83. The compound of item 77, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00460##

[1440] 84. The compound of item 77, wherein said compound has the structure of formula (Id):

##STR00461##

or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

[1441] 85. The compound of item 84, wherein said compound has the structure of formula (Ie):

##STR00462##

or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

[1442] 86. The compound of item 85, wherein said compound has the structure of formula (Ig):

##STR00463##

or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q.sup.a is a 4-9 membered saturated heterocyclyl.

[1443] 87. The compound of item 77, wherein said compound has the structure of formula (Ij):

##STR00464##

a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

[1444] 88. The compound of item 87, wherein said compound has the structure of formula (Ik):

##STR00465##

a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

[1445] 89. The compound of item 87, wherein said compound has the structure of formula (Ik):

##STR00466##

a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.

[1446] 90. The compound of any one of items 1-89, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein:

[1447] R.sup.9 is absent and ring A is a 4-8 membered heterocyclyl; or

[1448] R.sup.11 is N(C.sub.0-C.sub.5 alkylene-H), or N(C(O)(C.sub.0-C.sub.5 alkylene-H), wherein each alkylene portion of R.sup.11 is optionally substituted with one or more substituents independently selected from halo, CN, OH, C.sub.1-C.sub.3 alkyl, and OC.sub.1-C.sub.3 alkyl.

[1449] 91. The compound of any one of items 1-90, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein W is N(R.sup.12); and R.sup.13 is O.

[1450] 92. The compound of any one of items 1-35 and 39-83, wherein said compound has the structure of formula (IL):

##STR00467##

or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.18 is Br or Cl.

[1451] 93. The compound of any one of items 1-35 and 39-83, wherein said compound has the structure of formula (Im):

##STR00468##

or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.14 is H.

[1452] 94. The compound of any one of items 1-39, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of:

##STR00469## ##STR00470##

[1453] wherein:

[1454] 1 indicates a portion of Q bound to X; and

[1455] Q is further optionally substituted.

[1456] 95. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00471##

[1457] 96. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00472##

[1458] 97. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00473##

[1459] 98. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00474##

[1460] 99. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00475##

[1461] 100. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00476##

[1462] 101. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00477##

[1463] 102. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00478##

[1464] 103. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00479##

[1465] 104. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00480##

[1466] 105. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00481##

[1467] 106. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00482##

[1468] 107. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00483##

[1469] 108. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00484##

[1470] 109. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00485##

[1471] 110. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00486##

[1472] 111. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00487##

[1473] 112. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00488##

[1474] 113. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00489##

[1475] 114. The compound of item 94, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00490##

[1476] 115. The compound of any one of items 1-39, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is selected from the group consisting of:

##STR00491##

[1477] wherein:

[1478] R is CH.sub.2CH.sub.3,

CH.sub.2CH.sub.2OCH.sub.3, CH.sub.2CHF.sub.2, CH.sub.2CN, C(CH.sub.3).sub.2CN, C(CH.sub.3).sub.2CH.sub.2CN, CH.sub.2CH.sub.2CN, cyclohexyl, cyclobutyl, cyclopropyl, pyridin-4-yl, tetrahydropyran-4-yl, tetrahydropyran-4-ylmethyl, oxetan-3-ylmethyl, 2-cyano-5-methoxyphenyl, 2-cyano-5-methoxymethylphenyl, 2-cyano-6-(methoxymethyl)phenyl, 2-cyano-6-bromophenyl, 2-methoxyethan-1-yl, 2-cyanopropan-2-yl, 2-tetrahydropyran-4-ylethan-1-yl, 3-cyanopentan-3-yl, or 2-cyano-4-methoxybutan-2-yl, or

[1479] R is

##STR00492##

[1480] R.sup.23 is hydrogen or fluoro:

[1481] R.sup.24 is hydrogen,

chloro, CN, CH.sub.3, CH.sub.2CH.sub.3, CHF.sub.2, CF.sub.3, CH.sub.2CN, CH(CN)CH.sub.3, C(CH.sub.3).sub.2CN, C(CH.sub.2CH.sub.3).sub.2CN, CH.sub.2CH.sub.2CN, C(CH.sub.3)NOCH(CH.sub.3).sub.2, C(CH.sub.3)NOCH.sub.3, C(O)N(CH.sub.3).sub.2, C(O)NHCH.sub.3, OCH.sub.3, CH.sub.2OCH.sub.3, CCH, CCCH.sub.3, S(O).sub.2CH.sub.3, 1-(cyclopentyl)-1-cyanoethan-1-yl, 1-(tetrahydropyran-4-yl)-1-cyanoethan-1-yl, 1-(tetrahydrofuran-3-yl)-1-cyanoethan-1-yl, 1,3-dimethoxy-2-cyanopropan-2-yl, 1,4-dimethylpyrazol-5-yl, 1-cyanocyclobutyl, 1-cyanocyclopropyl, 1-cyanocylopentyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, 1-methylpyrazol-3-yl, 1-methylpyrazol-4-ylcyanomethyl, 1-methylpiperidin-4-yl, 1-methylpyrazol-5-yl, 1-oxoindolin-5-yl, 1-oxoisoindolin-4-yl, 1-oxoisoindolin-6-yl, 2-(2-methoxyethan-1-yl)phenyl, 2-(methoxymethyl)phenyl, 2-(tetrahydropyran-4-yloxy)phenyl, 2,2-difluoro-benzo[d][1,3]dioxol-4-yl, 2,3-dicyanopropan-2-yl, 2-chlorophenyl, 2-cyano-3-(tetrahydropyran-4-yl)propan-2-yl, 2-cyano-3-chlorophenyl, 2-cyano-3-fluorophenyl, 2-cyano-3-methoxyphenyl, 2-cyano-4-fluorophenyl, 2-cyano-4-chlorophenyl, 2-cyano-5-chlorophenyl, 2-cyano-5-fluorophenyl, 2-cyano-5-methoxyphenyl, 2-cyano-6-chlorophenyl, 2-cyano-6-fluorophenyl, 2-cyano-6-(tetrahydropyran-4-yloxy)phenyl, 2-cyanomethylphenyl, 2-cyanophenyl, 2-cyanopropan-2-yl, 2-cyclopentylphenyl, 2-difluoromethoxyphenyl, 2-fluorophenyl, 2-methoxy-6-cyanophenyl, 2-methoxyphenyl, 2-methoxycarbonylphenyl, 2-nitrophenyl, 2-oxopyrrolidin-1-yl, 2-phenoxyphenyl, 3-(1,1-dioxothiomorpholin-4-ylmethyl)phenyl, 3-(2-methoxyethan-1-yl)phenyl, 3,5-difluoro-4-(pyrrolidin-1-ylcarbonyl)phenyl, 3-cyano-2-methylpropan-2-yl, 3-cyanomethylphenyl, 3-cyanopentan-3-yl, 3-cyanophenyl, 3-hydroxy-2-methylbutan-2-yl, 3-hydroxy-3-methyl-but-1-yne-1-yl, 3-methoxy-2-methylbutan-2-yl, 3-methoxymethyl-5-methylisoxazol-4-yl, 3-methoxyphenyl, 3-methoxycarbonylphenyl, 3-oxo-2-methylbutan-2-yl, 4-cyanophenyl, 4-cyanotetrahydropyran-4-yl, 4-methoxyphenyl, benzo[d][1,3]dioxol-4-yl, benzo[d]oxazol-7-yl, benzo[d]thiazol-2-yl, benzo[d]thiazol-4-yl, benzo[d]thiazol-5-yl, benzo[d]thiazol-6-yl, benzo[d]thiazol-7-yl, cyclobutyl, cyclopropyl, cyclopropylcyanomethyl, N-methoxycyclopropanecarbimidoyl, phenyl, pyridin-2-ylmethyl, pyridin-3-yl, pyridin-3-ylmethyl, pyridin-4-ylmethyl, tetrahydrofuran-3-ylmethyl, tetrahydrofuran-3-ylcyanomethyl, tetrahydropyran-4-yl, or tetrahydropyran-4-ylcyanomethyl;

[1482] R.sup.27 is hydrogen, CH.sub.3, CHF.sub.2, CH.sub.2CH.sub.3, CH.sub.2OCH.sub.3,

CH.sub.2CN, CN, CH.sub.2O-CH.sub.2CN, C(O)N(CH.sub.3).sub.2, C(O)NHCH.sub.3, CH.sub.2OCH.sub.2CCH, 2-methoxyphenyl, 3-methoxyphenyl, 2,2-difluorobenzo[d][1,3]dioxol-4-yl, 2-cyanophenyl, 3-cyanophenyl, phenyl, 2-benzyl methyl ether, 2-(2-methoxyethyl) benzene, 2-(2-difluoromethoxymethyl)benzene, 2-(2-dimethylmethoxyethyl)benzene, pyridin-3-yl, pyridin-2-yl, pyridin-3-ylmethyl, or tetrahydropyridin-4-yl, or

[1483] R.sup.24 and R.sup.27 are taken together to form 4-cyanobenzene-1,2-diyl, 3-cyanobenzene-1,2-diyl, 5-methyl-5-cyanotetrahydropyran-3,4-diyl, 3-cyanocyclohexan-1,2-diyl, 3-methoxybenzene-1,2-diyl, benzene-1,2-diyl, 3-oxocyclohexyl-1,2-diyl, 3-cyanocyclopentan-1,2-diyl, or pyridin-3,4-diyl;

[1484] R.sup.28 is hydrogen, CH.sub.3, or CHOCH.sub.3; and

[1485] R.sup.11 is hydrogen, acetyl,

CN, CH.sub.2CN, CH.sub.2CH.sub.2CN, CH.sub.2OCH.sub.3, CHCHCN, CH.sub.2OC(O)N(CH.sub.3).sub.2, morpholin-4-ylmethyl, pyrazol-1-ylmethyl, pyridin-3-yl, pyridin-3-ylethynyl, pyridin-2-yloxymethyl, or 2-cyanopropan-2-yl, or

[1486] R.sup.28 and R.sup.29 are taken together to form 2,3-dihydrobenzofuran-3,3-diyl, 2,3-dihydrofuro[2,3-b]pyridin-3,3-diyl, tetrahydropyran-3,3-diyl, 6,7-dihydro-5H-cyclopenta[c]pyridin-6-yl, tetrahydropyran-4,4-diyl, or 4-methoxycyclohexane.

[1487] 116. The compound of item 115, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00493##

[1488] 117. The compound of item 115, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00494##

[1489] 118. The compound of item 115, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00495##

[1490] 119. The compound of item 115, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00496##

[1491] 120. The compound of item 115, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00497##

[1492] 121. The compound of item 115, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00498##

[1493] 122. The compound of item 115, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00499##

[1494] 123. The compound of item 115, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein Q is:

##STR00500##

[1495] 124. The compound of any one of items 1-123, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.1

is CH.sub.3, CH.sub.2CH.sub.3, (CH.sub.2).sub.2CH.sub.3, CH(CH.sub.3).sub.2, CH(CH.sub.3)CH.sub.2CH.sub.3, cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl, cyclohexylmethyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, phenyl, 4-methoxybenzyl, or tetrahydropyran-4-yl.

[1496] 125. The compound of any one of items 1-89, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.9 is absent and ring A is a saturated, nitrogen-containing heterocyclyl,

[1497] 126. The compound of any one of items 1-89 and 94-124, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 represented by:

##STR00501##

[1498] is selected from the group consisting of:

##STR00502## ##STR00503##

wherein each ring system in R.sup.2 is optionally substituted with up to 4 substituents independently selected from fluoro;
chloro; CN; OH; NH.sub.2; C.sub.1-C.sub.3 alkyl optionally substituted with CN, OH, NH.sub.2 or OC.sub.1-C.sub.3 alkyl; OC.sub.1-C.sub.3 alkyl; and NHC.sub.1-C.sub.3 alkyl,

[1499] 127. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is represented by:

##STR00504##

[1500] 128. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is represented by:

##STR00505##

[1501] 129. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00506##

[1502] 130. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00507##

[1503] 131. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00508##

[1504] 132. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00509##

[1505] 133. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00510##

[1506] 134. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00511##

[1507] 135. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00512##

[1508] 136. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00513##

[1509] 137. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00514##

[1510] 138. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00515##

[1511] 139. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00516##

[1512] 140. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00517##

[1513] 141. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00518##

[1514] 142. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00519##

[1515] 143. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00520##

[1516] 144. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00521##

[1517] 145. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00522##

[1518] 146. The compound of item 126, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein the portion of R.sup.2 is:

##STR00523##

[1519] 147. The compound of any one of items 1-89 and 94-124, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein: [1520] the portion of R.sup.2 represented by WH is C(O)CCCH.sub.3, C(O)CHCH.sub.2,
S(O).sub.2CHCH.sub.2, C(O)CH.sub.2Cl, C(O)CH(CH.sub.3)Cl, or C(O)CH(Cl)CH.sub.2OCH.sub.3, or

[1521] the portion of R.sup.2 represented by R.sup.11WH, when R.sup.11 is taken together with one R.sup.14 is

##STR00524##

[1522] 148. The compound of any one of items 1-147, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.2 is selected from the group consisting of: 1-(2-chloro-3-methoxypropanoyl)azetidin-3-yl-N-methylcarboxamido, 1-(2-chloroacetyl)azetidin-3-ylcarboxamido, 1-(2-chloroacetyl)azetidin-3-yl-N-ethylcarboxamido, 1-(2-chloroacetyl)azetidin-3-yl-N-methylcarboxamido, 1-(2-chloroacetyl) piperidin-3-yl-N-methylcarboxamido, 1-(2-chloroacetyl)piperidin-4-yl-N-methylcarboxamido, 1-(2-chloroacetyl)pyrrolidin-3-yl-N-methylcarboxamido. 1-(2-chloropropanoyl)-piperidin-4-yl-N-methylcarboxamido, 1-(2-chloropropanoyl)-3-fluoroazetidin-3-yl-N-methylcarboxamido, 1-(2-chloropropanoyl)azetidin-3-yl-N-methylcarboxamido, 1-(2-chloropropanoyl)pyrrolidin-3-yl-N-methylcarboxamido, 1-(but-2-ynoyl)-4-fluoropiperidin-4-ylcarbonylmethylamino, 1-(but-2-ynoyl)azetidin-2-yl-N-methylcarboxamido, 1-(but-2-ynoyl)azetidin-3-yl-N-methylcarboxamido, 1-(but-2-ynoyl)-piperidin-3-ylcarbonylmethylamino, 1-(but-2-ynoyl)-piperidin-4-ylcarbonylmethylamino, 1-(but-2-ynoyl)pyrrolidin-2-ylcarbonyl-N-methylamino, 1-(but-2-ynoyl)pyrrolidin-3-ylcarbonyl-N-methylamino, 1-acryloyl-2-oxo-imidazolidin-3-yl, 1-acryloyl-3-fluoroazetidin-3-yl-N-methylcarboxamido, 1-acryloyl-3-fluoropyrrolidin-3-yl-N-methylcarboxamido, 1-acryloyl-4-fluoropiperidin-4-ylcarbonylmethylamino, 1-acryloylazetidin-2-yl-N-methylcarboxamido, 1-acryloylazetidin-3-yl-N-methylcarboxamido, 1-acryloyl-piperidin-3-ylcarbonylmethylamino, 1-acryloyl-piperidin-4-ylcarbonylmethylamino, 1-acryloylpyrrolidin-2-yl-N-methylcarboxamido, 1-acryloylpyrrolidin-3-yl-N-methylcarboxamido, 1-oxo-7-(2-chloroacetyl)-2,7-diazaspiro[4.3]octan-2-yl, 1-oxo-7-(2-chloroacetyl)-2,7-diazaspiro[4.4]nonan-2-yl, 1-oxo-2-(2-chloroacetyl)-2,7-diazaspiro[4.5]decan-7-yl, 1-oxo-7-(2-chloroacetyl)-2,7-diazaspiro[4.5]decan-2-yl, 1-oxo-7-(2-chloropropanoyl)-2,7-diazaspiro[4.3]octan-2-yl, 1-oxo-7-(but-2-ynoyl)-2,7-diazaspiro[4.4]nonan-2-yl, 1-oxo-7-acryloyl-2,7-diazaspiro[4.3]octan-2-yl, 1-oxo-7-acryloyl-2,7-diazaspiro[4.4]nonan-2-yl, 1-oxo-7-acryloyl-2,7-diazaspiro[4.5]decan-2-yl, 1-oxo-8-(2-chloroacetyl)-2,8-diazaspiro[4.5]decan-2-yl, 1-oxo-8-(but-2-ynoyl)-2,8-diazaspiro[4.5]decan-2-yl, 1-oxo-8-acryloyl-2,8-diazaspiro[4.5]decan-2-yl, 1-vinylsulfonyl-2-oxoimidazolidin-3-yl, 1-vinylsulfonylazetidin-3-yl-N-methylcarboxamido, 2-(1-acryloylpiperidin-4-yl)-N-methylacetamido, 2-(but-2-ynoyl)-5-oxo-2,6-diazaspiro[3.4]octan-6-yl, 2,5-dioxo-3,4-dimethyl-2,5-dihydropyrrol-1-yl-N-methylacetamido, 2-acryloyl-2-azabicyclo[2.1.1]hexan-4-yl-N-methylcarboxamido, 2-chloroacetamidomethyl-N-methylcarboxamido, 2-oxo-2,5-dihydro-1H-pyrrol-1-yl-N-methylacetamido, 2-oxo-3-(2-chloroacetamido)pyrrolidin-1-yl, 2-oxo-3-(N-methyl-2-chloroacetamido)pyrrolidin-1-yl, 2-oxo-3-(N-methylacrylamido)pyrrolidin-1-yl, 2-oxo-3-acrylamidopyrrolidin-1-yl, 2-oxo-4-(2-chloroacetyl)piperazin-1-yl, 2-oxo-4-acryloylpiperazin-1-yl, 2-oxo-4-vinylsulfonylpiperazin-1-yl, 2-oxocyclopent-3-en-1-yl-N-methylacetamido, 3-(4-(dimethylamino)but-2-enamido)phenyl-N-methylcarboxamido, 4-(but-2-ynoyl)-piperazin-1-yl-N-methylcarboxamido, 4-acryloylpiperazin-1-yl-N-methylcarboxamido, 6-oxo-2-(2-chloroacetyl)-2,7-diazaspiro[4.5]decan-7-yl, and 6-oxo-2-acryloyl-2,7-diazaspiro[4.5]decan-7-yl.

[1523] 149. The compound of any one of items 1-85, 87, 88 and 90-148, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein:

[1524] R.sup.4 is hydrogen, fluoro, or CH.sub.3; and

[1525] R.sup.5 is hydrogen, fluoro,

chloro, OH, CH.sub.3, CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, CH.sub.2OH, CH.sub.2OCH.sub.3, CH.sub.2F, CHF.sub.2, CH.sub.2CN, CH.sub.2-cyclopropyl, cyclopropyl, pyridyl, phenyl, or CH.sub.2-phenyl, wherein any phenyl portion of R.sup.5 is optionally substituted with up to 4 substituents independently selected from halo, CN, and OC.sub.1-C.sub.3 alkyl; or

[1526] R.sup.4 and R.sup.5 are taken together to form CH.sub.2 or cyclopropyl, or cyclobutyl, or cyclopentyl, or cyclohexyl; or

[1527] R.sup.5 is taken together with the carbon atom to which it is bound, a ring atom of Q, and X to form oxazepane.

[1528] 150. The compound of any one of items 1-37 and 39-149, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, wherein R.sup.7 is OH, NH.sub.2, or CHF.sub.2.

[1529] 151. The compound of item 150, wherein R.sup.7 is OH.

[1530] 152. A compound, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, selected from FIG. 1.

[1531] 153. A pharmaceutical composition comprising a compound of any one of items 1-152, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, and a pharmaceutically acceptable carrier.

[1532] 154. A complex comprising a presenter protein, a RAS protein, and a compound of any one of items 1-152, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition of item 123.

[1533] 155. The complex of item 154, wherein the RAS protein is KRAS.

[1534] 156. The complex of item 154 or 155, wherein the RAS protein is KRAS G12C.

[1535] 157. The complex of any one of items 154-156, wherein the presenter protein is a cyclophilin.

[1536] 158. The complex of any one of items 154-157, wherein the presenter protein is CYPA, CYPB, CYPC, CYP40, CYPE, CYPD, NKTR, SRCyp, CYPH, CWC27, CYPL1, CYP60, CYPJ, PPIL4, PPIL6, RANBP2, or PPWD1.

[1537] 159. The complex of any one of items 154-158, wherein the presenter protein is CYPA.

[1538] 160. A method of producing a complex, the method comprising contacting a presenter protein and a KRAS G12C protein with a compound of any one of items 1-152, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, under conditions suitable to permit complex formation.

[1539] 161. The method of item 160, wherein the presenter protein is a cyclophilin protein.

[1540] 162. The method of item 160 or 161, wherein the presenter protein is PP1A, CYPA, CYPB, CYPC, CYP40, CYPE, CYPD, NKTR, SRCyp, CYPH, CWC27, CYPL1, CYP60, CYPJ, PPIL4, PPIL6, RANBP2, or PPWD1.

[1541] 163. The method of any one of items 160-162, wherein the presenter protein is CYPA.

[1542] 164. A method of treating cancer in a subject in need thereof, the method comprising administering to the subject an effective amount of a compound of any one of items 1-152, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, or a pharmaceutical composition of item 153.

[1543] 165. A method of inhibiting a KRAS G12C protein in a cell, the method comprising contacting the cell with an effective amount of a compound of any one of items 1-152, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, or a pharmaceutical composition of item 153.

[1544] 166. A method of treating a KRAS G12C protein-related disorder in a subject in need thereof, the method comprising administering to the subject an effective amount of a compound of any one of items 1-152, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, a pharmaceutical composition of item 153.

[1545] 167. A method of inhibiting RAF-RAS binding in a cell, the method comprising contacting the cell with an effective amount of a compound of any one of items 1-152, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, or a pharmaceutical composition of item 153.

[1546] 168. The method of item 165 or 167, wherein the cell is a cancer cell.

[1547] 169. The method of item 168, wherein the cancer cell is a colorectal cancer cell, a pancreatic cancer cell, or a non-small cell lung cancer cell.

[1548] 170. Use of a compound of any one of items 1-152, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, for treating cancer in a subject in need thereof.

[1549] 171. The method or use of item 164 or 170, wherein the cancer is pancreatic cancer, colorectal cancer, or non-small cell lung cancer.

[1550] 172. Use of a compound of any one of items 1-152, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof, for treating a KRAS G12C protein-related disorder in a subject in need thereof.

[1551] 173. The method or use of any one of items 164-172, wherein the method or use further comprises administering an additional therapeutic agent.

[1552] 174. The method of item 173, wherein the additional therapeutic agent is a HER2 inhibitor, an EGFR inhibitor, a second Ras inhibitor, a SHP2 inhibitor, a SOS1 inhibitor, a Raf inhibitor, a MEK inhibitor, an ERK inhibitor, a PI3K inhibitor, a PTEN inhibitor, an AKT inhibitor, an mTORC1 inhibitor, a BRAF inhibitor, a PD-L1 inhibitor, a PD-1 inhibitor, a CDK 4/6 inhibitor, or a combination thereof.

[1553] 175. The method of item 174, wherein the additional therapeutic agent is a SHP2 inhibitor.

[1554] 176. The method of item 175, wherein the SHP2 inhibitor is TNO155, JAB-3068, or RMC-4630.