Method for feeding Hermetia illucens and used as for preparing composite material of pupariums

Abstract

This invention discloses method for feeding Hermetia illucens and used as for preparing composite material of puparium, which comprises the following steps: S1, dry Hermetia illucens pupariums and grind them into powdery, S2, adding powdery of Hermetia illucens pupariums into sodium hydroxide aqueous solution, stirring, separating and filtering, S3, adding the pupariums into hydrochloric acid solution, stirring, separating and filtering; S4, placing the pupariums into sodium hydroxide aqueous solution, stirring, separating and filtering; S5, drying the pupariums, and screening out the granular pretreated powder of the Hermetia illucens pupariums. The invention also disclose preparation method of composite material and thin film of Hermetia illucens pupariums and antibacterial and antimildew additive. The method can effectively improve the yield of chitosan in Hermetia illucens pupariums, and the prepared pupariums powder can be used for preparing polymer composite fibers and thin films of the Hermetia illucens pupariums, thus, the antibacterial effect is greatly improved. A new antibacterial and antimycotic additive can be obtained by compounding the powder of Hermetia illucens pupariums with the powder of oyster shell.

Claims

1. Feeding method of Hermetia illucens, is characterized in that use starchy kitchen waste food or corn straw (inner) or mixed starchy kitchen waste food, distiller's grains and corn straw (inner) as feeds, and used as for preparation method of Hermetia illucens pupariums powder, is characterized in comprising the following steps: S1, dry Hermetia illucens pupariums and grind them into powdery, S2, adding powdery of Hermetia illucens pupariums into sodium hydroxide aqueous solution, stirring, separating and filtering, S3, adding the pupariums into hydrochloric acid solution, stirring, separating and filtering, S4, placing the pupariums into sodium hydroxide aqueous solution, stirring, separating and filtering, S5, drying the pupariums, and screening out the granular pretreated powder of the Hermetia illucens pupariums.

2. (canceled)

3. Preparation method of composite material of Hermetia illucens pupariums is characterized in that the method comprises the following steps of: mixing the pretreated powder of the Hermetia illucens pupariums with a polymer substrate according to the weight ratio of 0.1-25%, and then heating and melting the mixture.

4-8. (canceled)

9. A novel kind of antibacterial and antimildew additive is characterized in that the Hermetia illucens pupariums pretreatment powder is mixed with oyster shell powder, and the weight content of oyster shell powder is not less than 20%.

Description

4. BRIEF DESCRIPTION OF THE FIGURES

[0026] FIG. 1 is a schematic diagram of a specific embodiment of the black hydra pupa shell composite material after forming fibers.

[0027] FIG. 2 is a schematic diagram of a specific manufacturing process of the black water fly pupa shell powder according to the present invention.

[0028] FIG. 3 is a schematic diagram of the fiber making process of the composite material of black hydra pupa shell according to the present invention.

[0029] Wherein:

[0030] 1—Hermetia illucens pupariums polymer composite fiber

[0031] 2—Polymer substrate

[0032] 20—polymer composite material

[0033] 3—Hermetia illucens pupariums powder.

[0034] 30—Hermetia illucens pupariums

[0035] 301—The pupariums powder of the black Hermetia illucens treated in S2.

[0036] 302—The pupariums powder of the Hermetia illucens treated in S3.

[0037] 303—The pupariums powder of the Hermetia illucens treated in S4.

[0038] 4—Grinding machine.

[0039] 5—Mixer.

[0040] 51—Low concentration sodium hydroxide solution.

[0041] 52—Hydrochloric acid solution.

[0042] 53—High concentration sodium hydroxide solution.

[0043] 6—Vibration screening machine.

[0044] 7—Macromolecular composite material of Hermetia illucens pupariums.

5. DESCRIPTION

[0045] With reference to the accompanying drawings, the specific embodiments of the present invention will be further described in detail below.

Embodiment 1

[0046] Five groups of Hermetia illucens were fed separately with material described as below:

[0047] (1) Starch waste residue, such as starch kitchen waste such as noodles, bread, steamed bread and leftovers;

[0048] (2) Distiller's grains

[0049] (3) Straw

[0050] (4) Meat

[0051] (5) Livestock manure

[0052] The Hermetia illucens pupariums left by the adults of the black hydra in five feeding ways were collected.

[0053] The Hermetia illucens pupariums powder was prepared in the following manner:

[0054] S1, drying Hermetia illucens pupariums, and grinding into pow by grinding machine,

[0055] S2, adding Hermetia illucens pupariums powder into 3.5 wt % low-concentration sodium hydroxide solution 51, stirring by a mixer 5, and then separating and filtering. The main function of this step is to remove the protein on the surface of pupariums.

[0056] S3, adding the Hermetia illucens pupariums powder obtained in step S2 into 12 equivalent concentration of hydrochloric acid solution 52, stirring, and separating and filtering.

[0057] The main function of this step is to remove the mineral salt components in the pupariums.

[0058] S4, putting the Hermetia illucens pupariums obtained in step S3 into 30 wt % high-concentration sodium hydroxide solution 53, stirring, separating and filtering.

[0059] S5, drying the Hermetia illucens pupariums obtained in the step S4, crushing and grinding the dried black hydra pupa shell into particles with required particle size or Hermetia illucens pupariums pretreatment powder.

[0060] By adopting the above method, the preparation of pupariums powder can be quickly and simply completed, and high-purity chitosan can be extracted, and a relatively high yield can be obtained compared with the traditional method.

[0061] Table 1 shows the yield of chitosan in the Hermetia illucens pupariums powder prepared by the above method and the traditional method:

TABLE-US-00001 TABLE 1 The method of Traditional Food types of Hermetia illucens this invention(%) method(%) Starchy kitchen waste such as 38.8 30.4 noodles, bread, steamed bread and leftovers (a) Distiller's grains (b) 28.3 21.5 Corn Straw (c) (skin and stalk 23.9 17.3 attached Meat kitchen waste such as pork. 16.6 11.7 Beef, chicken (f) Livestock manure (g) 8.9 4.2

[0062] From Table 1, it can be seen that different food sources will affect the yield of chitosan extracted from the pupa shell of the Hermetia illucens, among which the food source of starch waste residue is the best, corn straw (inner) (d) Inner stalk of straw is the second, distiller's grains is the third, and the yield of livestock manure is the lowest. Compared with the traditional preparation method, the yield of chitosan obtained by treating the pupa shells of the hydra in the present invention shows that the yield of the new process of the present invention is increased by about 5-8% compared with that of the traditional process under the same food source. Compared with the traditional process, the new process greatly reduces the process flow, the process is not complicated, and it is more suitable for industrialization.

Embodiment 2

[0063] Melt-blend the Hermetia illucens pupariums powder prepared in embodiment 1 (a) with different polymer substrates, such as polypropylene, polyamine, polylactic acid, polyethylene terephthalate, etc., to prepare different Hermetia illucens pupariums polymer composite materials, so that the Hermetia illucens pupariums 3 can be uniformly distributed in the polymer composite material 20. The specific method is to mix the polymer substrate powder with the black fly pupa shell powder uniformly.

[0064] Then, through spinning by thermoplastic equipment, etc., the polymer composite fiber 1 of Hermetia illucens pupariums as shown in FIG. 1 can be produced, and the obtained composite fiber can be further made into a non-toxic, safe, antibacterial, breathable macromolecular composite material of Hermetia illucens puparium 7 with good mechanical properties and elongation, as shown in FIG. 3.

[0065] When the polymer substrate does not contain the Hermetia illucens pupariums powder, it has no antibacterial effect. When the mass ratio of Hermetia illucens pupariums powder in the mixture reaches 0.1%, the composite material begins to show antibacterial effect. When the mass ratio of Hermetia illucens pupariums powder in the mixture reaches 20%, the antibacterial rate of all the composite materials except polypropylene-based composite material is 99.6%. The relative cell proliferation rate, which reflects the cell activity, is also greatly improved with the increase of the content of the Hermetia illucens pupariums powder. The specific test parameters are shown in Table 2.

TABLE-US-00002 TABLE 2 Content of Hermetia illucens Relative cell Types of polymer pupariums Antibacterial proliferation substrates powder(%) ratio(%) rate(%) Polypropylene 0 0 103.1 Polypropylene 0.1 7.3 107.1 Polypropylene 0.5 88.8 111.3 Polypropylene 20 99.6 178.9 Polypropylene 0 0 116.2 Polypropylene 0.1 14.4 118.1 Polypropylene 0.5 95.1 122.3 Polypropylene 20 100 209.1 Polylactic acid 0 0 258.9 Polylactic acid 0.1 19.2 263.6 Polylactic acid 0.5 91.7 289.5 Polylactic acid 20 100 333.3 Polyethylene glycol 0 0 210.2 terephthalate Polyethylene glycol 0.1 11.8 211.4 terephthalate Polyethylene glycol 0.5 88.3 224.9 terephthalate Polyethylene glycol 20 100 299.3 terephthalate

Embodiment 3

[0066] Dissolving 14.925 g and 12 g of carboxymethyl cellulose in 100 ml aqueous solution respectively, and 0.075 g and 3 g of Hermetia illucens pupariums powder prepared in embodiment 1 (a) were added to the aqueous solution of carboxymethyl cellulose respectively. The powder was dispersed in carboxymethyl cellulose by stirring at a high speed of 1000 rpm, and several films were scraped with a scraper with a thickness of 600 um, and dried in an oven for 8 hours to prepare composite film of Hermetia illucens pupariums with content of Hermetia illucens pupariums reaches 0.5% and 20% respectively. The method for preparing the film can also be tape casting, in which the powder is melted and plasticized from an extruder, extruded through a slit die, so that the melt is tightly attached to a cooling roller, and the sheet is made by stretching, trimming, coiling and other processes.

[0067] The specific test performance is shown in Table 3. The carboxymethyl cellulose film without adding the powder of the black fly pupa shell has no antibacterial effect, and the antibacterial rate increases with the increase of the powder content of the Hermetia illucens pupariums. As the Hermetia illucens pupariums is a natural biological material, its cell activity also increases with the increase of the content of the Hermetia illucens pupariums.

TABLE-US-00003 TABLE 3 Content of Hermetia illucens Relative cell pupariums Antibacterial proliferation powder(%) ratio(%) rate(%) Carboxymethylcellulose 0 0 233.1 Carboxymethylcellulose 0.1 13.3 237.1 Carboxymethylcellulose 0.5 93.9 265.5 Carboxymethylcellulose 20 100 302.4

Embodiment 4

[0068] Polyol polybutylene glycol adipic acid, polyether glycol and internal emulsifier dimethylolpropionic acid are added into a four-mouth separated reaction tank equipped with a fixed-speed stirrer, a temperature controller and the like, and a mixture is obtained after reacting at 105° C. for 20 minutes, and water is removed by separation; Then, when the temperature is lowered to 80° C., the pupa shell of black hydra is added, and hydrogenated phenyl methane diisocyanate, 2,6-pyridine dimethanol and several drops of dibutyltin dilaurate catalyst diluted to 5% with DMAC (dimethylacetamide) are added at the rotating speed of 300 rpm.

[0069] Reacting in a reactor for 2.5 hours to obtain a prepolymer with NCO (isocyanate) groups at the terminal, then dispersing into aqueous PU dispersion by acetone processing method; When the temperature is reduced to 55° C., triethylamine is added for neutralization reaction for 20 minutes, then, increasing the rotating speed to 1000 rpm, adding deionized water to a concentration of 65 wt %, and stirring until the polymer is uniformly dispersed in water. Finally, the chain extender ethylenediamine is added for chain extension reaction for 30 minutes. The obtained product is pyridine-containing waterborne polyurethane with solid content of 35 wt %.

[0070] According to the weight of 0.1% and 20% of the solid content of the waterborne polyurethane, the powder of the Hermetia illucens pupariums prepared in embodiment 1 (a) was added into the waterborne polyurethane containing pyridine for high-speed stirring at 1000 rpm, and then several films were scraped by a scraper with a thickness of 600 um, and dried in an oven for 8 hours to prepare the black fly pupa shell composite film. The antibacterial rate and the relative cell proliferation rate of the product are tested as shown in Table 4.

TABLE-US-00004 TABLE 4 Content of Hermetia illucens Relative cell pupariums Antibacterial proliferation powder(%) ratio(%) rate(%) Polyurethane 0 0 174.5 Polyurethane 0.1 9.1 174.9 Polyurethane 0.5 90.2 192.3 Polyurethane 20 100 235.7

Embodiment 5

[0071] Dissolving 29.85 g and 24 g of polyvinyl alcohol in 200 ml aqueous solution at 85° C., 0.15 g and 6 g of Hermetia illucens pupariums powder prepared in embodiment 1 (a) were added to the aqueous solution of polyvinyl alcohol, and the powder was dispersed in carboxymethyl cellulose by stirring at 500 rpm. Several films were scraped with a scraper with a thickness of 600 um, and dried in an oven at 80° C. for 10 hours, the composite films of Hermetia illucens pupariums with the Hermetia illucens pupariums content of 0.1% and 20% respectively were prepared.

[0072] The specific test performance is shown in Table 3. The polyvinyl alcohol film without adding Hermetia illucens pupariums powder prepared in embodiment 1 (a) has no antibacterial effect, and the antibacterial rate increases with the increase of the powder content of the Hermetia illucens pupariums. As Hermetia illucens pupariums is a natural biological material, its cell activity also increases with the increase of the content of the Hermetia illucens pupariums powder.

TABLE-US-00005 TABLE 5 Content of Hermetia illucens Relative cell pupariums Antibacterial proliferation powder(%) ratio(%) rate(%) Polyvinyl alcohol 0 0 238.7 Polyvinyl alcohol 0.1 7.3 241.2 Polyvinyl alcohol 0.5 94.3 256.8 Polyvinyl alcohol 20 100 311.5

[0073] The foregoing are all the preferred embodiments of the present invention. If the preferred embodiments in each preferred embodiment are not obviously contradictory or based on a certain preferred embodiment, each preferred embodiment can be used in any superposition and combination. The embodiments and the specific parameters in the embodiments are only for clearly describing the inventor's invention verification process, and are not used to limit the patent protection scope of the present invention. The patent protection scope of the present invention shall still be subject to its claims. Any equivalent structural changes made by using the contents of the specification and drawings of the present invention shall be equally applicable.

Embodiment 6

[0074] The Hermetia illucens pupariums gel was prepared by dissolving the Hermetia illucens pupariums powder prepared in embodiment 1 (a) in 1.8% acetic acid aqueous solution. The calcined oyster shell powder was added into the Hermetia illucens pupariums with the ratio of 20%, 40%, 60% and 80%, respectively. First, it was vibrated and dispersed for 10 minutes with high-sound cell breaker to avoid agglomeration of oyster shell powder. According to the antibacterial and anti-mildew tests of oyster shell powder with different proportions, Table 6 shows that although the Hermetia illucens pupariums has good antibacterial performance, it has no good anti-mildew effect. With the increase of oyster shell powder content, its anti-mildew effect will be better. This novel composite bio-based powder can be used as an excellent antibacterial and antimycotic additive.

TABLE-US-00006 TABLE 6 Relative cell Content of oyster Antibacterial proliferation shell powder (%) ratio(%) rate(%) Hermetia illucens 0 99.9 16.8 pupariums Hermetia illucens 20 99.9 63.5 pupariums Hermetia illucens 40 99.9 97.3 pupariums Hermetia illucens 60 99.9 99.9 pupariums Hermetia illucens 80 99.9 99.9 pupariums

[0075] The above are various embodiments of the present invention, and their descriptions are more specific and detailed, but they cannot be understood as limiting the patent scope of the present invention. It should be pointed out that for those of ordinary skill in the field, without departing from the concept of the present invention, several modifications and improvements can be made, which all belong to the protection scope of the present invention. Therefore, the scope of protection of the present invention shall be subject to the appended claims.