CANNABINOID NANOMICELLE PREPARATION AND METHOD FOR PREPARING SAME

Abstract

The invention discloses a cannabinoid nano-micelle preparation and a preparation method thereof. The cannabinoid nano-micelle preparation includes cannabinoid and an amphiphilic polymer, wherein the content of the cannabinoid is 1-40% by weight, the content of the amphiphilic polymer is 1-99%, and the preparation method includes the following steps: (1) preparing a cannabinoid nano-micelle solution from cannabinoid and an amphiphilic polymer; (2) drying the micellar solution obtained in the step (1) to obtain cannabinoid nano-micelle powder; and (3) preparing the cannabinoid nano-micelle powder obtained in the step (2) into the cannabinoid nano-micelle preparation. The cannabinoid nano-micelle preparation is high in effective component wrapping rate and transfer rate, high in drug loading capacity and high in stability, and a novel normal-temperature self-assembly technology is adopted, so that an active component cannabinoid is prevented from being degraded and discolored at high temperature; the bioavailability of the active ingredient is high, and a single dose can be reduced. Especially, a dry powder inhalant is high in in-vitro deposition rate and quick in inhalation effect, and can provide continuous and stable blood concentration.

Claims

1. A cannabinoid nano-micelle preparation, comprising cannabinoid and an amphiphilic polymer; wherein the content of the cannabinoid is 1-40% by weight, and the content of the amphiphilic polymer is 1-99%, and a preparation method of the preparation comprises the following steps: (1) preparing a cannabinoid nano-micelle solution from cannabinoid and an amphiphilic polymer; (2) diluting the micellar solution obtained in the step (1), and drying to obtain cannabinoid nano-micelle powder; and (3) preparing the cannabinoid nano-micelle powder obtained in the step (2) into the cannabinoid nano-micelle preparation.

2. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the cannabinoid is selected from one or a combination of two or more of pure products of cannabidiol (CBD), cannabidivarin (CBDV), cannabigerol (CBG), cannabichromene (CBC), cannabinol (CBN), cannabidibutol (CBDB), cannabielsoin (CBE), cannabicyclol (CBL) and cannabinodiol (CBND); or, the cannabinoid is a cannabis extract, and comprises one or a combination of two or more of CBD, CBDV, CBG, CBC, CBN, CBDB, CBE, CBL and CBND.

3. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the amphiphilic polymer is vitamin E polyethylene glycol succinate.

4. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the amphiphilic polymer is amphiphilic polyurethane; the amphiphilic polyurethane is obtained by alternately copolymerizing a polyethylene glycol chain segment and a raw material comprising diisocyanate.

5. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the amphiphilic polymer is selected from one or a combination of two or more of poloxamer, a polylactic acid-polyethylene glycol-polylactic acid triblock copolymer, a polyethylene glycol-polyacrylic acid block copolymer, a polyethylene glycol-polyaspartic acid block copolymer, a polyethylene glycol-poly(lactic-co-glycolic acid) block copolymer, a polyethylene glycol-polycaprolactone block copolymer, a polyethylene glycol-polylactic acid block copolymer and a polyethylene glycol-polystyrene block copolymer.

6. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the cannabinoid nano-micelle preparation further comprises a pharmaceutically acceptable freeze-drying protective agent, wherein the content of the freeze-drying protective agent is 1-10%, and the freeze-drying protective agent is selected from one or more of lactose, mannitol, sorbitol, cyclodextrin, hydroxypropyl-β-cyclodextrin, EDTA-2Na, trehalose, glucose, xylitol and maltose.

7. The cannabinoid nano-micelle preparation according to claim 6, characterized in that the cannabinoid nano-micelle preparation further comprises a pH regulator, wherein the content of the pH regulator is 0.01-10%, and the pH regulator is selected from one or more of citric acid, sodium citrate, tartaric acid, sodium tartrate, acetic acid, sodium acetate, sodium dihydrogen phosphate, disodium hydrogen phosphate, hydrochloric acid, lactic acid and sodium hydroxide.

8. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the step (1) comprises the following steps: (1-1) adding raw material components of the cannabinoid nano-micelle preparation into a solvent for swelling; (1-2) shearing the mixture obtained in the step (1-1) by using a high-shear dispersing emulsifier to obtain an emulsion; and (1-3) stirring the emulsion obtained in the step (1-2) to obtain a clear transparent nano-micelle solution.

9. The cannabinoid nano-micelle preparation according to claim 8, characterized in that a mass ratio of the solvent to the raw material components in the step (1-1) is (2-10):1; the swelling in the step (1-1) is performed for 0.1-2 h; the shearing in the step (1-2) is performed for 1-30 min; and the stirring in the step (1-3) is performed at 15-35° C.

10. The cannabinoid nano-micelle preparation according to claim 8, characterized in that the cannabinoid nano-micelle solution has a particle size of 1-500 nm.

11. The cannabinoid nano-micelle preparation according to claim 1, characterized in that a dilution ratio in the step (2) is 2-100.

12. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the drying in the step (2) is atomization freeze drying or freeze drying.

13. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the preparation is a solid preparation or a semi-solid preparation.

14. The cannabinoid nano-micelle preparation according to claim 1, characterized in that the preparation is an oral preparation, a preparation for mucosal administration or a preparation for transdermal administration.

15. A preparation method of the cannabinoid nano-micelle preparation according to claim 1, comprising the following steps: (1) preparing a cannabinoid nano-micelle solution from cannabinoid and an amphiphilic polymer; (2) diluting the micellar solution obtained in the step (1), and drying to obtain cannabinoid nano-micelle powder; and (3) preparing the cannabinoid nano-micelle powder obtained in the step (2) into the cannabinoid nano-micelle preparation; wherein the step (1) comprises the following steps: (1-1) adding raw material components of the cannabinoid nano-micelle preparation according to any one of claims 1-9 into a solvent for swelling; (1-2) shearing the mixture obtained in the step (1-1) by using a high-shear dispersing emulsifier to obtain an emulsion; and (1-3) stirring the emulsion obtained in the step (1-2) to obtain a clear transparent nano-micelle solution.

16. The preparation method according to claim 15, characterized in that a mass ratio of the solvent to the raw material components in the step (1-1) is (2-10):1; the swelling in the step (1-1) is performed for 0.1-2 h; the shearing in the step (1-2) is performed for 1-30 min; and the stirring in the step (1-3) is performed at 15-35° C.

17. The preparation method according to claim 15, characterized in that a dilution ratio in the step (2) is 2-100.

18. The preparation method according to claim 15, characterized in that the drying in the step (2) is atomization freeze drying or freeze drying.

19. The preparation method according to claim 18, characterized in that the atomization freeze drying comprises the steps of atomization, freezing and drying, wherein an atomization mode is selected from one or a combination of two or more of pneumatic atomization, pressure atomization, centrifugal atomization and ultrasonic atomization; the freezing is performed at −10° C. to −50° C.; and the drying step is performed under a vacuum degree of 40 Pa or below at 10-35° C.

20. The preparation method according to claim 18, characterized in that the freeze drying comprises the steps of pre-freezing, sublimation drying and desorption drying; wherein the pre-freezing step is performed at −30° C. to −50° C. for 0.5-3 h by controlling a vacuum degree to 1-100 Pa; the sublimation drying is performed at −20° C. to 10° C. for 1-36 h; and the desorption drying is performed at 10-30° C. for 1-24 h.

Description

DETAILED DESCRIPTION

[0081] Unless otherwise defined, all scientific and technical terms used in the invention have the same meaning as those generally understood by those skilled in the technical field to which the present invention relates, for example, the following abbreviations and corresponding substances appearing in the invention are as follows:

TABLE-US-00001 CBDV Cannabidivarin CBD Cannabidiol CBG Cannabigerol CBN Cannabinol CBC Cannabichromene CBDB 4-butyl-5′-metyl-2′-(prop-1-en-2-yl)-1′,2′,3′,4′- tetrahydro-(1,1′-bipheyl)-2,6-diol, Cannabidibutol CBE Cannabielsoin CBL Cannabicyclol CBND Cannabinodiol TPGS vitamin E polyethylene glycol succinate Poloxamer Poloxamer PEG-PAA Polyethylene glycol-polyacrylic acid block copolymer PEG-PASP Polyethylene glycol-polyaspartic acid block copolymer PEG-PLAG Polyethylene glycol-poly(lactic-co-glycolic acid) block copolymer PEG-PCL Polyethylene glycol-polycaprolactone block copolymer PEG-PLA/PTX Polyethylene glycol-polylactic acid block copolymer PEG-b-PS Polyethylene glycol-polystyrene block copolymer SFD Spray freeze drying

[0082] In the invention, the term ‘cannabinoid’ refers to a class of secondary metabolites containing a molecular structure of alkyl and a monoterpene group unique to cannabis plants, for example, CBD, CBDV, CBG, CBC, CBN, CBDB, CBE, CBL, CBND and the like (as described in “Xuan Chen, Ming Yang, Hongyan Guo, Research Advances in Cannabinoids of Cannabis sativa, Botanical Newton, 2011, 46 (2): 197-205”).

[0083] In the invention, pure products of CBD, CBDV, CBG, CBC, CBN, CBDB, CBE, CBL and CBND refer to pure products of the compounds, especially corresponding commercially available products, and the purity of the compounds is at least 99.5% or above, especially 99.9% or above (the balance is impurities).

[0084] The technical solution of the invention is clearly and completely described in combination with the embodiments of the invention, and obviously, the described embodiments are only a part of the embodiments of the invention instead of all of the embodiments of the invention. Based on the embodiments of the present invention, other embodiments obtained by those of ordinary skill in the art without creative work all belong to the scope of protection of the present invention.

Embodiment 1: Preparation of Cannabinoid Nano-micelle Solution

[0085] 1. A specific formula of the cannabinoid nano-micelle solution is shown in the following table.

TABLE-US-00002 TABLE 1 Formula of cannabinoid nano-micelle solution TPGS Poloxamer Polyurethane PEG 2000- PEG 2000- PEG 1000- Formula CBD 1000 188 4000 PAA 2000 PASP 1000 PLAG 3000 1 10% 10% 50% 0 0 0 10% 2 20% 0 0 10% 0 0 0 3 30% 0 0 0 10% 10% 0 4 40% 0 0 0 0 0 10% 5 50% 0 0 0 0 10% 0 6 60% 0 10% 10% 0 0 0 PEG 1000- PEG 3400- PEG 5000- Citric Tartaric Formula PCL 2000 PLA/PTX 4000 b-PS 5000 Mannitol Sorbitol acid acid 1 0 0 0 5% 5% 1% 0 2 50% 0 0 5% 5% 0 1% 3 0 30% 0 5% 5% 1% 0 4 10% 0 20% 5% 5% 0 1% 5 0 10% 10% 5% 5% 1% 0 6 0 0 0 5% 5% 0% 1% Hydroxypropyl- EDTA-2 Formula Cyclodextrin β-cyclodextrin Na Trehalose Leucine Lactose 1 0 5% 0.1% 1% 0.5% 2.4% 2 5% 0 0 1% 0 .sup. 3% 3 0 5% 0.1% 1% 0.5% 2.4% 4 5% 0 0 1% 0 .sup. 3% 5 0 5% 0.1% 1% 0.5% 2.4% 6 5% 0 0 1% 0 .sup. 3%

[0086] 2. Preparation process

[0087] (1) A first set of preparation processes (process 1)

[0088] The cannabinoid nano-micelle solution is prepared by feeding according to the proportion in formulae 1-6, specifically as follows:

[0089] a. swelling: 100g of raw materials are taken according to the formula in Table 1, and added into 800 g of water for swelling for 2 h;

[0090] b. emulsifying: the obtained mixture after swelling is sheared for 30 min by using a high-shear dispersing emulsifier at 50 Hz; and

[0091] c. stirring: the temperature of the emulsification system is controlled at 30° C., and stirring is performed for 48 h to obtain a clear transparent nano-micelle solution with a particle size of less than 500 nm.

[0092] The quality comparison of the cannabinoid nano-micelle solution obtained by the above preparation process is shown in the following table.

TABLE-US-00003 TABLE 2 Quality comparison of cannabinoid nano-micelle solution Quality of corresponding solution Effective Effective Solution component ingredient Solution particle Solution wrapping transfer Formula clarity size color rate % rate % 1 Clear and 0-500 nm Colourless 100% 100% transparent 2 Clear and 0-500 nm Colourless 100% 100% transparent 3 Milky white 0-500 nm White  90% 100% liquid 4 Clear and 0-500 nm Colourless 100% 100% transparent 5 Clear and 0-500 nm Colourless 100% 100% transparent 6 Milky white 0-500 nm White  90% 100% liquid

[0093] (2) A second set of preparation processes (process 2)

[0094] The cannabinoid nano-micelle solution is prepared by feeding according to the proportion in formulae 1-6, specifically as follows:

[0095] a. melting: TPGS 1000, Poloxamer 188, polyurethane 4000, PEG 2000-PAA 2000, PEG 2000-PASP 1000, PEG 1000-PLAG 3000, PEG 1000-PCL 2000, PEG 3400-PLA/PTX 4000 and PEG 5000-b-PS 5000 are added according to the formulae 1-6 in Table 1, and melted at 70° C., cannabinoid and a monomer thereof are added after melting, and uniform dispersing is performed to prepare a liquid 1;

[0096] b. dissolving: according to the formulas 1-6 in Table 1, mannitol, sorbitol, citric acid, tartaric acid, cyclodextrin, hydroxypropyl-β-cyclodextrin, EDTA-2Na, trehalose, leucine and lactose are dissolved with 8 times of water to prepare a liquid 2; and

[0097] c. mixing and dissolving: the liquid “1” and the liquid “2” are mixed and dissolved at 40° C., and slow stirring is performed for 12 h until a particle size of the solution is less than 500 nm.

[0098] The quality comparison of the cannabinoid nano-micelle solution obtained by the above preparation process is shown in the following table.

TABLE-US-00004 TABLE 3 Quality comparison of cannabinoid nano-micelle solution Quality of corresponding solution Effective Effective Solution component ingredient Solution particle Solution wrapping transfer Formula clarity size color rate % rate % 1 Clear and 0-500 nm Colourless 100% 97% transparent 2 Clear and 0-500 nm Colourless 100% 97% transparent 3 Milky white 0-500 nm White  90% 96% liquid 4 Clear and 0-500 nm Colourless 100% 97% transparent 5 Clear and 0-500 nm Colourless 100% 97% transparent 6 Milky white 0-500 nm White  90% 96% liquid

[0099] It can be seen from the results in Table 2 and Table 3 that under the same formula, the transfer rate of the cannabinoid nano-micelle prepared by the first set of processes is higher than that of the cannabinoid nano-micelle prepared by the second set of processes, which may be due to the melting step adopted in the second set of processes, which makes cannabinoid degrade.

Embodiment 2: Preparation of Cannabinoid Nano-micelle Powder

[0100] A drying mode for preparing micelle powder includes vacuum drying, atomization freeze drying, nano spray drying, supercritical fluid technology and freeze drying, the formula 1 prepared by the first set of processes in Embodiment 1 is prepared into cannabinoid nano-micelle powder by the above drying techniques, respectively, and a quality comparison study is carried out, and a specific drying mode comparison is shown in Table 4.

[0101] Atomization freeze drying: the cannabinoid nano-micelle solution is sprayed into an atomization freeze dryer to produce the cannabinoid micelle micropowder, and a specific process is as follows:

[0102] a. atomization:

[0103] the nano-micelle solution prepared by the process 1 in Embodiment 1 is diluted to 100 times by using purified water, and subjected to atomization freeze drying by using an atomization freeze dryer with an atomization device adopting ultrasonic atomization.

[0104] b. freezing:

[0105] the solid content is 40% when pneumatic atomization, pressure atomization and centrifugal atomization are adopted, and the solid content is 10% when ultrasonic atomization is adopted;

[0106] Feeding flow rate: 10 ml/min;

[0107] Freezing temperature: −45° C.;

[0108] c. drying:

[0109] Vacuum degree: 40 Pa or below;

[0110] Drying temperature: 30° C.

[0111] A freeze-drying process: the cannabinoid nano-micelle solution is put into a freeze-drying oven for freezing at −40° C. for 3 h by controlling a vacuum degree to be less than 10 Pa, wherein primary drying is performed at −10° C. for 24 h; and secondary drying is performed at 25° C. for 12 h; and the dried material is sieved and subjected to straightening granulating through No.1-No.5 sieves.

[0112] The quality comparison of the powder prepared by the above drying processes is shown in the following table.

TABLE-US-00005 TABLE 4 Quality comparison of powder obtained by different drying processes In-vitro Particle size deposition determination Drying Process Powder Surface distribution Emptying Deposition mode controllability hygroscopicity % Solubility morphology d (0.9) μm rate % rate % Vacuum Simple and 2.0% the solution Needle-like 50.1 μm 75%  5% drying controllable is clear Atomization Simple and 1.0% the solution Spherical 5 μm 95% 45% freeze drying controllable is clear Nano spray Simple and 1.3% the solution Flaky 20 μm 90% 30% drying controllable is clear Supercritical Complicated and 2.0% the solution Flaky 15 μm 89% 29% fluid uncontrollable is clear Freeze drying Simple and 1.5% the solution Flaky 48 μm 70%  4% controllable is clear A detection method of the above parameters is as follows: 1. Hygroscopicity: a certain amount of dry powder is weighed, and placed in a test tube (with an outer diameter of 50 mm and a height of 15 mm), the test tube which is opened is placed for 24 h in an environment with a temperature of 25° C. and a relative humidity of 70%, the test tube is weighed and a moisture absorption weight gain is calculated. 2. Solubility: 0.2 g of dry drug powder is taken, and put into a test tube, 5 mL of pure water is added, oscillating is performed, and the dissolution condition of the dry drug powder is observed. If the dry drug powder can be dissolved to obtain a clear solution, the drug has good solubility and can be used as a pulmonary inhalation preparation, and if the dry drug powder cannot be dissolved, the drug has poor water solubility and is not suitable for being used as a pulmonary inhalation preparation. 3. Surface morphology: the contact points among spherical particles are minimum, the dispersibility is best, and more plane contact points exist among particles in the shapes of flakes, needles and the like, so that the flowability is relatively poor. 4. Particle size distribution: represented by d (0.9) μm, and the particle size of 90% of particles is less than this particle size. The particles of 1-5 μm can enter the lung through the respiratory airflow, and a good lung deposition rate is achieved. 5. In-vitro deposition rate determination: the in-vitro deposition rate determination is carried out by using NGI at 60 L/min, including an emptying rate % and an effective deposition rate %, the emptying rate of a solid powder inhalation preparation is not lower than 90%, and the deposition rate is greater than 30%.

[0113] Result analysis: through the comparative analysis of several drying modes from the aspects of process controllability, powder hygroscopicity, solubility, surface morphology, particle size distribution, in-vitro deposition rate determination and other indicators, it is determined that atomization freeze drying has more advantages in inhalation preparation than other powders, and freeze-dried powder is more suitable for the preparation of other solid preparations.

Embodiment 3: Cannabinoid Nano-micelle Inhalation Preparation

[0114] The powder obtained by atomization freeze drying in Embodiment 2 has a particle size of 1-5 μm, the surface morphology is spherical, the fluidity is better, the emptying rate is 95%, the deposition rate is 45%, the powder is suitable for being used as inhalation powder, and the powder is filled into No.2-No.00 capsules, vesicles and reservoirs or can be directly used for administration by inhalation.

[0115] The bioavailability comparison between the cannabinoid nano-micelle inhalation powder and the micellar solution prepared according to the method described in the embodiments of the patent application WO2019008178A1 is shown in the following table.

TABLE-US-00006 TABLE 5 Bioavailability comparison Bioavailability Item AUC Inhalation powder prepared by this solution 60% Micellar solution prepared according to the patent 18% WO2019008178A1

Embodiment 4: Preparation of Cannabinoid Nano-micelle Effervescent Tablets

[0116] 1. Formula: cannabinoid micelle powder (powder obtained by a freeze drying process in Embodiment 2), DL-tartaric acid, citric acid, sodium bicarbonate, mannitol, lactose, aspartame, xylitol and polyethylene glycol. A specific formula is shown in the following table.

TABLE-US-00007 TABLE 6 Formula of cannabinoid nano-micelle effervescent tablets Cannabinoid DL- micelle tartaric Citric Sodium Polyethylene Formula powder acid % acid % bicarbonate % Mannitol % Lactose % Aspartame % Xylitol % glycol % 1 10% 20% 5% 25% 5% 20% 1% 5% 9% 2 20% 20% 5% 25% 0 15% 1% 5% 9% 3 30% 20% 5% 25% 0 10% 1% 4% 5%

[0117] Specification: the tablet weight is 2 g.

[0118] 2. Preparation process

[0119] According to the formula in Table 6, the cannabinoid micelle powder, DL-tartaric acid, citric acid, mannitol and polyethylene glycol are prepared into particles 1 by using a fluidized bed or by wet granulation; sodium bicarbonate, aspartame, lactose, xylitol and polyethylene glycol are prepared into particles 2 by using a fluidized bed or by wet granulation; and the particles 1, the particles 2 and the cannabinoid micelle powder are uniformly mixed, and pressed into effervescent tablets, wherein the tablet weight is controlled to be 2 g.

[0120] The quality comparison of the effervescent tablets of different formulas is shown in the following table.

TABLE-US-00008 TABLE 7 Quality comparison of cannabinoid nano-micelle effervescent tablets Solution state Disintegration Dispersion after effer- Content Formula time/min uniformity vescence stability 1 2 Qualified Clear and The content is transparent stable after tablets are subjected to accelerated test for 6 months 2 3 Qualified Clear and The content is transparent stable after tablets are subjected to accelerated test for 6 months 3 5 Qualified Clear and The content is transparent stable after tablets are subjected to accelerated test for 6 months

[0121] Result analysis: the effervescent tablets all meet the requirements of the effervescent tablet quality standard, the effervescent tablets are quickly dissolved in water and convenient to use and carry, the dissolving speed of cannabinoid powder can be increased, and the effervescent solution is a cannabinoid nano-micelle solution which can be directly taken.

Embodiment 5: Preparation of Cannabinoid Nano-micelle Sustained Release Tablets

[0122] 1. Formula: cannabinoid micelle powder (powder obtained by a freeze drying process in Embodiment 2), hydroxypropyl methylcellulose, polyvinylpyrrolidone, ethyl cellulose, lactose, superfine silica powder and magnesium stearate. A specific formula is shown in the following table.

TABLE-US-00009 TABLE 8 Formula of cannabinoid nano-micelle sustained release tablets Cannabinoid Superfine micelle Hydroxypropyl Polyvinyl- Ethyl silica Magnesium Formula powder methylcellulose pyrrolidone cellulose Lactose powder stearate 1 10% 50%  9% 9% 20% 1% 1% 2 30% 20% 10% 10%  28% 1% 1% 3 50% 20% 10% 6% 12% 1% 1%

[0123] 2. Preparation process: according to the formula in Table 8, hydroxypropyl methylcellulose, polyvinylpyrrolidone, ethyl cellulose, lactose and tartaric acid are added, and granulated with a certain amount of absolute ethyl alcohol, cannabinoid micelle powder, superfine silica powder and magnesium stearate are added, uniform mixing is performed, and the obtained mixture is pressed into 1 g of sustained release tablets.

[0124] The quality comparison of the sustained-release tablets of different formulas is shown in the following table.

TABLE-US-00010 TABLE 9 Quality comparison of cannabinoid nano- micelle sustained release tablets Tablet weight Hardness In vitro Formula Properties variation kg/cm.sup.2 dissolution Stability 1 White to ±2.5% 9.0 Linear Stable after brown release being tablets with a subjected to cumulative accelerated release rate test for 6 of 92% months 2 White to ±2.0% 9.2 Linear Stable after brown release being tablets with a subjected to cumulative accelerated release test for 6 degree months of 90% 3 White to ±2.6% 9.1 Linear Stable after brown release being tablets with a subjected to cumulative accelerated release rate test for 6 of 91% months In vitro dissolution: 6 cannabinoid nano-micelle sustained release tablets in a same batch are taken and put into a rotating basket, a rotating speed of the rotating basket is set to be 120 rpm, the temperature is set to be 37.0 ± 0.5° C., and the cumulative release rate of linear release of a sample within 12 h is 90% or more by taking 1000 mL of fresh degassed distilled water as a dissolution medium.

[0125] The properties, tablet weight variation, hardness, in vitro dissolution and stability of the sustained-release tablets in the formulae all meet the requirements, cannabinoid can be slowly released in the intestinal tract, cannabinoid nano-micelles can slowly release cannabinoid after entering the blood, the dual sustained-release effect is achieved, the cannabinoid can maintain stable blood concentration in vivo for a long time, and the sustained-release tablets take effect on chronic pains for a long time.

Embodiment 6: Preparation of Cannabinoid Nano-micelle Orally Disintegrating Tablets

[0126] 1. Formula: cannabinoid micelle powder (powder obtained by a freeze drying process in Embodiment 2), L-HPC, lactose, microcrystalline cellulose, pregelatinized starch, superfine silica powder and magnesium stearate. A specific formula is shown in the following table.

TABLE-US-00011 TABLE 10 Formula of cannabinoid nano-micelle orally disintegrating tablets Cannabinoid Superfine micelle Microcrystalline Pregelatinized silica Magnesium Formula powder L-HPC Lactose cellulose starch powder stearate 1 10% 30% 30% 19% 10%  0.5% 0.5% 2 30% 30% 20% 10% 9% 0.5% 0.5% 3 50% 25% 15%  5% 4% 0.5% 0.5%

[0127] 2. Preparation process

[0128] The materials are prepared into granules through a wet method or one-step granulation, and the granules are pressed into 0.5 g of rapidly disintegrating tablets.

[0129] The quality comparison of the quick-release tablets of different formulas is shown in the following table.

TABLE-US-00012 TABLE 11 Mass comparison of cannabinoid nano-micelle quick-release tablets Tablet Disintegration weight Formula Properties time S variation Stability 1 White to 40S ±2.5% Stable after brown being subjected tablets to accelerated test for 6 months 2 White to 45S ±2.3% Stable after brown being subjected tablets to accelerated test for 6 months 3 White to 48S ±2.2% Stable after brown being subjected tablets to accelerated test for 6 months

[0130] The properties, tablet weight variation, disintegration time and stability of the orally disintegrating tablets of the three formulas all meet requirements, the orally disintegrating tablets can be rapidly disintegrated in the mouth and absorbed into blood under the tongue after being taken in the mouth, the first-pass effect is avoided, and the orally disintegrating tablets are convenient for patients with dysphagia to take.

Embodiment 7: Preparation of Cannabinoid Nano-micelle Gel

[0131] 1. Formula: cannabinoid micelle powder (powder obtained by a freeze drying process in Embodiment 2), carbomer, glycerol, propylene glycol, triethanolamine, EDTA-2Na and ethylparaben. A specific formula is shown in the following table.

TABLE-US-00013 TABLE 12 Formula of cannabinoid nano-micelle gel Cannabinoid micelle Propylene Formula powder Carbomer Glycerol glycol Triethanolamine EDTA-2Na Ethylparaben 1 10% 10% 47% 28.9% 3% 0.1% 1% 2 30% 12% 30% 23.9% 3% 0.1% 1% 3 50% 13% 20% 11.9% 4% 0.1% 1%

[0132] 2. Preparation process: according to the formula in Table 12, 100 times of water is added into carbomer for swelling, triethanolamine is added to form a gel matrix, then the cannabinoid micelle powder, glycerol, propylene glycol, EDTA-2Na and ethylparaben are added, and uniform stirring is performed to obtain the cannabinoid nano-micelle gel.

[0133] The mass comparison of gel of different formulas is shown in the following table.

TABLE-US-00014 TABLE 13 Quality comparison of cannabinoid nano-micelle gel Formula Properties Uniformity Stability 1 Colorless to Evenly Stable after being brown paste dispersed and subjected to solid delicate accelerated test for 6 months 2 Colorless to Evenly Stable after being brown paste dispersed and subjected to solid delicate accelerated test for 6 months 3 Colorless to Evenly Stable after being brown paste dispersed and subjected to solid delicate accelerated test for 6 months

[0134] The properties, uniformity and stability of the gel in the three formulas meet the requirements, and the gel can be directly smeared on the affected part to achieve the effects of treating dermatitis and joint chronic pain, removing acnes and freckles and the like.

Embodiment 8: Preparation of Cannabinoid Nano-micelle Dropping Pills

[0135] 1. Formula: cannabinoid micelle powder (powder obtained by a freeze drying process in Embodiment 2), polyethylene glycol 4000 and polyethylene glycol 6000. A specific formula is shown in the following table.

TABLE-US-00015 TABLE 14 Formula of cannabinoid nano-micelle dropping pills Cannabinoid Polyethylene Polyethylene micelle glycol glycol Water Formula powder 4000% 6000% % 1 10% 40% 45% 5% 2 30% 25% 30% 5% 3 50% 20% 25% 5%

[0136] 2. Preparation process: according to the formula in Table 14, the cannabinoid micelle powder, polyethylene glycol 4000, polyethylene glycol 6000 and purified water are heated at 70° C. to be melted to prepare slurry 1, the condensation temperature of paraffin oil is controlled to be 10° C., the slurry 1 is put into a pill dropping machine, dropping is performed to obtain dropping pills of 50 mg/pill, and the paraffin oil on the surfaces of the dropping pills is removed by using oil absorption cotton to obtain the cannabinoid nano-micelle dropping pills.

[0137] The quality comparison of the dropping pills of different formulas is shown in the following table.

TABLE-US-00016 TABLE 15 Quality comparison of cannabinoid nano-micelle dropping pills Pill weight Disintegration Formula Properties difference time/min Stability 1 White to ±8% 10 min Stable after being yellowish- subjected to brown accelerated test pills for 6 months 2 White to ±7% 9 min Stable after being yellowish- subjected to brown accelerated test pills for 6 months 3 White to ±8% 11 min Stable after being yellowish- subjected to brown accelerated test pills for 6 months

[0138] The properties, pill weight difference, disintegration time and stability of the dropping pills in the three formulas all meet the requirements, and the dropping pills can be taken orally or sublingually, and is quick in effect and lasting in blood concentration.

Embodiment 9: Preparation of Cannabinoid Nano-micelle Suppository

[0139] 1. Formula: cannabinoid micelle powder (powder obtained by a freeze drying process in Embodiment 2), cocoa butter, mixed fatty acid glyceride, polyethylene glycol, and ethylparaben. A specific formula is shown in the following table.

TABLE-US-00017 TABLE 16 Formula of cannabinoid nano-micelle suppository Cannabinoid Mixed fatty Poly- micelle Cocoa acid ethylene Ethyl- Formula powder butter glyceride glycol paraben 1 10% 50% 30% 9.5% 0.5% 2 30% 40% 20% 9.5% 0.5% 3 50% 25% 20% 4.5% 0.5%

[0140] 2. Preparation process: according to the formula in the above table, the cannabinoid micelle powder, cocoa butter, mixed fatty acid glyceride, polyethylene glycol and ethylparaben are melted at 40-60° C., and the melted material is put into a suppository preparation machine to prepare suppositories such as spherical suppositories, bullet suppositories, capsule suppositories and the like.

[0141] The quality comparison of the suppositories of different formulas is shown in the following table.

TABLE-US-00018 TABLE 17 Quality comparison of cannabinoid nano-micelle suppositories Weight Melting Formula Properties difference % time/min Stability 1 White to 4.1% 25 min Stable after being brown subjected to suppository accelerated test for 6 months 2 White to 4.0% 26 min Stable after being brown subjected to suppository accelerated test for 6 months 3 White to 4.5% 27 min Stable after being brown subjected to suppository accelerated test for 6 months

[0142] The properties, the weight difference, the melting time and the stability of the suppositories in the three formulas all meet the requirements, and the suppositories can directly reach focuses to achieve the anti-inflammatory effect or be used for systemic administration to treat chronic pains, so that the application range of cannabinoid is expanded.

[0143] The above is only better embodiments of the invention, and is not intended to limit the invention, and any modification, equivalent replacement and the like made within the spirit and principle of the invention should be included in the protection scope of the invention.