Fluid handling systems and method for ultracentrifuges

Abstract

A fluid handling system is provided that includes a controller, a plurality of valves, a pump, and an interface in communication with the controller. The valves can be operatively connected with a conduit to along a flow path. Each of the valves is controlled by the controller for selective movement between a first position and a second position. The first position closes the flow path, while the second position opens the flow path. The pump can be operatively connected with the conduit and the is controlled by the controller for selective pumping of fluid through the flow path. The interface allows selective definition of the flow path through the valves.

Claims

1. A fluid handling system, comprising: a controller; a plurality of valves configured for operative connection with a conduit along a flow path, each of the plurality of valves being controlled by the controller for selective movement between a first position and a second position, the first position closing the flow path, the second position opening the flow path; a pump configured for operative connection with the conduit, the pump being controlled by the controller for selective pumping of fluid through the flow path; and an interface in communication with the controller, the interface being configured to selectively define the flow path from among a plurality of flow paths through the plurality of valves, wherein the plurality of valves each comprises a light, and wherein the controller is configured to provide, by controlling the light, a visual indication of whether the conduit is to be installed in a particular valve to define the fluid path from among the plurality of flow paths wherein the plurality of valves each comprises a light, the light being controlled by the controller to provide a visual indication of whether the conduit is to be installed in a particular valve to define the fluid path from among the plurality of flow paths.

2. The fluid handling system of claim 1, wherein the system is a self-contained workstation.

3. The fluid handling system of claim 1, wherein the conduit comprises flexible conduit, wherein the plurality of valves comprises a plurality of pinch valves and the pump comprises a peristaltic pump, the plurality of pinch valves and the peristaltic pump being configured for operative connection to the flexible conduit.

4. The fluid handling system of claim 1, further comprising a sensor selected from the group consisting of a flow sensor, a pressure sensor, and a temperature sensor, a refractometer, and any combinations thereof, wherein the interface is configured to selectively define the flow path through the sensor.

5. The fluid handling system of claim 1, further comprising a refractometer controlled by the controller, the refractometer comprises an inline device that measures the state of the fluid through a transparent portion of the conduit, wherein the interface is configured to selectively define the flow path through the refractometer.

6. The fluid handling system of claim 5, wherein the state of the fluid measured by the refractometer comprises an index of refraction and/or a temperature.

7. The fluid handling system of claim 1, further comprising at least one additional device controlled by the controller, the at least one additional device being selected from the group consisting of a pressure sensor, a flow transducer, a scale, and any combinations thereof, wherein the interface is configured to selectively define the flow path through the at least one additional device.

8. The fluid handling system of claim 1, wherein the controller comprises a programmable logic controller (PLC) and is configured to allow visual programming of the PLC.

9. A fluid handling system, comprising: a controller; a plurality of pinch valves each configured for operative connection with a conduit, each of the plurality of pinch valves being controlled by the controller for selective movement between an open position and a closed position; a peristaltic pump configured for operative connection with the conduit, the peristaltic pump being controlled by the controller for selective pumping of fluid through the conduit; an interface in communication with the controller, the interface being configured to allow selective definition of a selected flow path through the plurality of valves and the pump from a plurality of possible flow paths; and a light on each of the plurality of valves, wherein the controller is configured to provide, by controlling the light, a visual indication of which valves of the plurality of valves the conduit is to be installed to define the selected flow path during a conduit installation process.

10. The fluid handling system of claim 9, wherein the light is further controlled by the controller to indicate whether the valve is in the open position or the closed position during use.

11. The fluid handling system of claim 9, wherein the light is further controlled by the controller to illuminate proportionally with respect to a state of the valve.

12. The fluid handling system of claim 9, further comprising a sensor controlled by the controller, wherein the sensor is selected from the group consisting of a flow sensor, a pressure sensor, and a temperature sensor, a refractometer, and any combinations thereof, wherein the interface is configured to selectively define the selected flow path through the sensor.

13. The fluid handling system of claim 9, further comprising a refractometer controlled by the controller, the refractometer comprising an inline device that measures a state of fluid through a transparent portion of the conduit, wherein the interface is configured to selectively define the selected flow path through the refractometer.

14. The fluid handling system of claim 9, wherein the state of the fluid comprises an index of refraction and/or a temperature.

15. The fluid handling system of claim 9, wherein the interface is configured to allow selective definition of the selected flow path by selection from the plurality of flow paths that are pre-programmed on the controller.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is a schematic view of a fluid handling system according to the present disclosure in use with an ultracentrifuge;

(2) FIG. 2 is a schematic depiction of a system architecture of the fluid handling system of FIG. 1;

(3) FIG. 3 is a perspective view of the fluid handling system of FIG. 1;

(4) FIG. 4 is a magnified view of the fluid handling system of FIG. 1;

(5) FIG. 5 is an exploded view of a pinch valve according to the present disclosure;

(6) FIG. 6 is a schematic depiction of program interface illustrating a pre-programmed mode of operation;

(7) FIG. 7 is a flow diagram of a method programming interface process;

(8) FIG. 8 is a schematic depiction of program interface illustrating the method programming mode of operation;

(9) FIG. 9 is a schematic depiction of program interface illustrating the method programming mode of operation; and

(10) FIG. 10 is a schematic depiction of a state editing mode of operation.

DETAILED DESCRIPTION OF THE INVENTION

(11) Referring to the drawings and in particular to FIGS. 1 and 2, a fluid handling system according to the present disclosure is shown and is generally referred to by reference numeral 10.

(12) Advantageously and as described in more detail herein, system 10 supports a plurality of pinch valves for routing of fluid, pumps for flow and direction, an inline refractometer for fractionation/monitoring and disposable flow, pressure and temperature transducers for process monitoring. System 10 includes software that includes pre-programmed operations and allows for the creation of customized methods for automation of various flow sequences such as sanitization, rinsing, filling, fractionation and other custom methods. Moreover, system 10 is configured to integrate with the customer's network and can utilize upstream and downstream inputs and outputs via OPC.

(13) System 10 is illustrated in use with an ultracentrifuge 12, which includes a control cabinet 14 and a rotor/tank system 16. System 10 is self-contained workstation 18 that supports automated filling and fractionation of fluids to and from ultracentrifuge 12 by, for example, communicating with control cabinet 14 of the ultracentrifuge 12. It should be recognized that system 10 is illustrated by way of example only in use with ultracentrifuge 12. Of course, it is contemplated by the present disclosure for system 10 to find use with any vessels, containers, or processing devices.

(14) Workstation 18 is compact and, preferably, configured to be mobile and/or configured for use in an industrial cleanroom environment. For example, workstation 18 can be made of stainless steel 303/304 and can be configured to meet or exceed standards such as, but not limited to, NEMA 4 an IP65. Workstation 18 can, in some embodiments, allow the position of the front panel to be adjusted with respect to the support legs. Thus, system 10 via the configuration of workstation 18 provides an ergonomic and compact design to fit even in a small clean room and to be able to pass through a commonly used egress doorway as small as 32 inches, is configured to easy cleaning by, for example, hand wiping, and provides all controls sufficient for activation via personnel wearing personal protective equipment (e.g., two Latex/Nitrile gloves).

(15) Thus, system 10 includes a computer 20 for communicating with ultracentrifuge 12 and/or any other customer network 22 in a wired and/or wireless manner.

(16) Systems 10 includes, resident on workstation 18, computer 20 in communication with a programmable logic controller (PLC) 24. Computer 20 includes an OPC communication software 26 resident thereon for wired and/or wireless communication with customer network 22, PLC 24, and one or more human-machine-interface (HMI) 28. HMI 28 can be any interface such as, but not limited to, a keyboard, mouse, touchscreen, printer, display screen, speaker, notification device (e.g., lights, alarm, etc.), tablet, laptop, buttons, or any other interface.

(17) Additionally, computer 20 includes a fluid handling control software interface 30 resident thereon, which advantageously allows the operator to control PLC 24 to operate one or more devices 32 in a pre-programmed mode of operation, program system 10 using method programming process, operate system 10 using a state editing mode of operation, and any combinations thereof, which will be described in more detail below. Devices 32 can include sensors, pumps, valves, lights, data collection, and others.

(18) System 10 is illustrated in FIG. 2 by way of example having specific communication protocols (e.g., Ethernet, USB, etc.) among computer 20, customer network 22, PLC 24, HMI 28, and devices 32. Of course, it is contemplated by the present disclosure for system 10 to communicate among the various components in any desired manner.

(19) System 10, by separating the control of devices 32 via PLC 24 from the communication with HMI 28 via computer 20 allows for redundancy within the system. For example, failure of HMI 28 during use will not cause failure or ceasing of operations by computer 20 or workstation 14. Rather, a user can simply access computer 20 and PLC 24 via connection of another HMI.

(20) Referring now to FIGS. 3 and 4, system 10 will be described in more detail.

(21) System 10 is configured to fluidly communicate with ultracentrifuge 12 via conduit 40. Conduit 40 can be disposable, reusable, and combinations thereof. System 10 includes, as one or more of devices 32, an array of pinch valves 42 controlled by PLC 24 to selectively open and close conduit 40. Here, conduit 40 at least in the portions acted upon by valves 42 is configured as resilient tubing. In the illustrated embodiment, system 10 includes thirteen normally closed pinch valves 42, but of course it is contemplated by the present disclosure for system 10 to support any desired number or configuration of valves.

(22) System 10 further includes, as one or more of devices 32, a pump 44 controlled by PLC 24 to pump fluid through conduit 40. In some embodiments, pump 44 can be a peristaltic pump that acts on conduit 40 in a known manner. In this embodiment, conduit 40 at least in the portions acted upon by pump 44 is configured as resilient tubing.

(23) System 10 further includes, as one or more of devices 32, a refractometer 46 controlled by PLC 24 to detect a state of fluid in conduit 40. In some embodiments, refractometer 46 can be an inline device that senses the state of fluid in conduit 40. Conduit 40 can be configured, at least in the region of refractometer 46, to have transparency sufficient for measurement of the fluid. The state of fluid measured by refractometer 46 can include, but is not limited to, index of refraction and temperature.

(24) System 10 further includes, as one or more of devices 32, one or more of a pressure sensor 48, a flow transducer 50, and a scale 52 controlled by PLC 24 to detect a state of fluid in conduit 40.

(25) Of course, it should be recognized that devices 32 are described above as by way of example. However, it is contemplated by the present disclosure for system 10 to have any desired number or configuration of devices 32 controlled by PLC 24 as necessary for the particular use of the system.

(26) Accordingly, system 10 is configured for control of flow when loading rotor 16 with, for example, water for injection (WFI), gradient materials, analyte materials, process materials, sampling and/or unloading of the rotor materials during fraction collection, and others. In this manner, system 10 can be easily configured to allow the user to fill collection devices 54 in communication with conduit 40 such as, but not limited to, reservoirs, jars, bottles, bags, and the like.

(27) System 10 can load collection devices 54 according to variables such as, but not limited to, refractive index, volume, mass, time, a particular event or detected state of control cabinet 14, rotor 16, system 10, fluid in conduit 40, and others.

(28) In this manner, system 10, having computer 20 resident thereon, operates independent from, but in communicating with ultracentrifuge 12 or a plurality of centrifuges.

(29) However, it is also contemplated by the present disclosure for system 10 to operate dependently with ultracentrifuge 12. For example and referring again to FIG. 2, it is contemplated by the present disclosure for computer 20 and OPC communication software 26 resident thereon to be present in control cabinet 14 of ultracentrifuge 12 instead of workstation 18. In this embodiment, computer 20 can be in wired and/or wireless communication with PLC 24 of workstation 18.

(30) The construction and operation of each of the valves 42 is described in more detail with respect to FIG. 5.

(31) Valve 42 includes a solenoid 60 controlled by PLC 24. Solenoid 60 can be an electro-mechanical solenoid, a pneumatic solenoid, or others. Solenoid 60 is housed partially within workstation 18 and extends through one or more openings 62 in the workstation for operative coupling with conduit 40.

(32) Valve 42 further includes a pinch valve head 64 positioned outside of workstation 18 and operatively connected to solenoid 62 so that movement of the solenoid by PLC 24 causes the head to pinch conduit 40 to close the fluid path in a first position or to release the conduit to open the fluid path in a second position. Openings 62 in workstation 18 are sealed or otherwise closed by head 64 and, in some embodiments, first seal 66.

(33) In embodiments where conduit 40 is disposable, the conduit is preferred to be made entirely of a soft resilient tubing. However, it is contemplated by the present disclosure for conduit 40, even when disposable, to have one or more sections formed of rigid tubingwith the portions of the conduit at valves 42 and/or pump 44 having sufficient flexibility and resiliency to function in the intended manner. Moreover, it is contemplated by the present disclosure for conduit 40 to be rigid tubing with in communication with valves 42, which can be any non-pinch valve design configured for operation by solenoid 60 such as, but not limited to ball valves, needle valves, cup valves, and the like.

(34) Valve 42 can further include one or more lights 68 (only one shown) in communication with PLC 24. Light 68 can be a light emitting diode (LED) or any other light device. Light 68 can include a cover or protective lens 70 secured to head 64 by a second seal 72. Light 68 can be controlled to provide a visual indication as to when the valve is open or closed.

(35) For example, light 68 can be controlled to be on (i.e., illuminate) when valve 42 is in the first position and can be controlled to be off (i.e., not illuminated) when the valve is in the second position. Of course, this operation can be reversed in some embodiments.

(36) In other embodiments, light 68 can illuminate in two different colors representative of the first and second positions, respectively. In still other embodiments, light 68 can include two different lighting elements that illuminate in different colors representative of the first and second positions, respectively.

(37) It should be recognized that valve 42 is described as using light 68 to indicate only first and second positions of the valve, namely where the on and off state of the light corresponds to the open and closed states of the valve. Of course, it is contemplated by the present disclosure for valve 42 to be configured so that light 68 illuminates proportionally with respect to the state of the valve, namely with an intensity or number of lights corresponding the proportion of openness of the valve.

(38) In some embodiments, light 68 can be controlled directly by the position of solenoid 60, while in other embodiments the light can be controlled by PLC 24.

(39) In this manner, system 10 is configured to provide the user with a visual indicationviewable from a distance such as from outside the clean room of the flow path through the system during use.

(40) Additionally, and in some embodiments where it is necessary to install disposable conduit 40, system 10 can be controlled to illuminate the valves 42 into which the conduit is to be installed. Furthermore, PLC 24 can be controlled so that the view of the flow path on HMI 28 mirrors that of lights 68.

(41) In some embodiments, head 64 includes a conduit receiving opening 74 into which conduit 40 can be removably received.

(42) System 10 can be configured to operate in a pre-programmed or main mode 80, which is illustrated with reference to FIG. 6. Here, system 10 can include one or more pre-programmed control schemes resident on computer 20 and/or PLC 24. In the illustrated example, a user can selectvia HMI 28the use of pre-programmed mode 80 and once selected can select a particular program 82 shown as a load buffer/gradient program from among a plurality of different the pre-programmed control schemes.

(43) In response, to selection of program 82, HMI 28 can illustrate to the user the operation of system 10 and PLC 24 can control devices 32 according to the program. When lights 68 are present, PLC 24 can also provide the visual indication of the state of valves 42.

(44) In this way, the normal operation of system 10 can be selected by the user during the pre-programmed mode 80. Here, the user can, for example, enter via HMI 28 unique batch identifying information, which system 10 can compare to information present on customer network 22 related to the batch, and can either load program 82 based on information from the customer network or allow the operator to continue upon verification of proper batch information. Once the desired program 82 has been selected and commenced, system 10 canwhere installation of conduit 40 is requiredwill open valves 42 and/or light lights 68 to prompt the operator to install the conduit. After confirmation of proper setup, system 10 as controlled by PLC 24 will control devices 32 according to program 82.

(45) System 10 can also be configured to operate in a method programming mode 84, which is illustrated with reference to FIGS. 7-9. Without wishing to be bound by any particular theory, it has been determined by the present disclosure that the language necessary for PLC 24 to operate devices 32 can be difficult to understand and/or program. Thus, method programming mode 84 advantageously provides a way for the operator to program PLC 24 using software 26 resident on computer 20 and, not, via the PLC language. Mode 84 provides a visual method of programming tasks into system 10.

(46) Here, the method editor of programming mode 84 allows the user to list sequential operations or steps of a particular desired program 86. This is a dynamic method editor the user can change, and is not hard coded so that the operator can define the control steps of devices 32. Programming mode 84 allows the operator to create, edit and disable programs 86. While working on a method, system 10 allows the operator to add, insert, delete or reorder the steps 88 of program 86 while providing a visual indication via HMI 28 of the various device 32 and their states of operation. Upon completion of programming mode 84, computer 20 will store the program 86 and control PLC 24 to execute the program to control devices 32 as desired.

(47) System 10 can also be configured to operate in a state editing mode 90, which is illustrated with reference to FIG. 10. Here, the operator can select the state editing mode 90 from the main sequence editor (FIG. 6). Here, any programs 82 that were selected or programs 86 that were designed can be manually overridden by the operator. For example, FIG. 10 illustrates manual overrides via state editing mode 90 of pump 44. Although illustrated with respect to pump 44, it is contemplated by the present disclosure for editing mode 90 to find use with the control of any device 32 within system 10.

(48) It should also be noted that the terms first, second, third, upper, lower, and the like may be used herein to modify various elements. These modifiers do not imply a spatial, sequential, or hierarchical order to the modified elements unless specifically stated.

(49) While the present disclosure has been described with reference to one or more exemplary embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the present disclosure. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the disclosure without departing from the scope thereof. Therefore, it is intended that the present disclosure not be limited to the particular embodiment(s) disclosed as the best mode contemplated, but that the disclosure will include all embodiments falling within the scope of the present invention.

(50) TABLE-US-00001 PARTS LIST system 10 ultracentrifuge 12 control cabinet 14 rotor/tank system 16 self-contained workstation 18 computer 20 customer network 22 programmable logic controller (PLC) 24 communication software 26 human-machine-interface (HMI) 28 fluid handling control software interface 30 devices 32 conduit 40 pinch valves 42 pump 44 refractometer 46 pressure sensor 48 flow transducer 50 scale 52 collection devices 54 solenoid 60 openings 62 pinch valve head 64 first seal 66 light 68 cover or protective lens 70 second seal 72 conduit opening 74 pre-programmed or main mode 80 particular program 82 method programming mode 84 particular desired program 86 steps 88 state editing mode 90