Metribuzin tolerance alleles in soybean

Abstract

Methods for conveying metribuzin resistance and/or tolerance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing metribuzin resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with metribuzin resistance and/or tolerance. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance or tolerance to metribuzin. Soybean plants and seeds produced by any of the disclosed methods are provided.

Claims

1. A method of producing a soybean plant with increased tolerance to metribuzin, the method comprising the steps of: a. isolating a nucleic acid from a soybean plant or plant part; b. detecting in the nucleic acid of a) a molecular marker within a soy chromosomal interval corresponding to soybean chromosome 3 and corresponding to an A allele at position 473 of SEQ ID NO: 1 or a G allele at position 650 of SEQ ID NO: 2, wherein said molecular marker is associated with increased metribuzin tolerance; c. selecting a first soybean plant on the basis of the presence of said molecular marker of b); d. crossing the first soybean plant of c) with a second soybean plant not comprising the molecular marker detected in b); and e. thereby, producing a soybean plant having increased metribuzin tolerance.

2. The method of claim 1, wherein said chromosomal interval has both alleles corresponding to the A allele at position 473 of SEQ ID NO. 1 and the G allele at position 650 of SEQ ID NO. 2.

3. The method of claim 1, wherein the chromosome interval comprises an interval defined by and including physical map positions 41,147,377 and 43,504,700.

4. The method of claim 1, wherein detecting is carried out by way of PCR or through molecular probes.

5. A method of producing a soybean plant having increased tolerance to metribuzin, the method comprising the steps of: a. isolating a nucleic acid from a soybean plant part; b. detecting in the nucleic acid of a) a molecular marker within a soy chromosomal interval corresponding to soybean chromosome 3 between and including physical map positions 41,147,377 and 43,504,700, wherein said molecular marker is associated with increased tolerance to metribuzin, and wherein said molecular marker corresponds to at least one of an A allele at position 473 of SEQ ID NO: 1 or a G allele at position 650 of SEQ ID NO: 2; c. selecting/identifying a soybean plant on the basis of the presence of said molecular marker of b); d. crossing the soybean plant of c) with a second soybean plant not comprising the molecular marker of a) e. producing progeny plants from the cross of d) thereby, producing a soybean plant having increased tolerance to metribuzin.

6. A method of producing a soybean plant (Glycine max) having increased tolerance to metribuzin, the method comprising the steps of: a. using a genome editing process to edit an allele on soybean chromosome 3 to an allele that is associated with increased tolerance to metribuzin wherein said allele is any one of the following an A allele at position 473 of SEQ ID NO. 1 and a G allele at position 650 of SEQ ID NO. 2; and b. thereby producing a soybean plant having increased tolerance to metribuzin.

7. The method of claim 6, wherein the genome editing process includes at least one of CRISPR, TALEN or Mega nuclease.

Description

EXAMPLES

(1) The following Examples provide illustrative embodiments. In light of the present disclosure and the general level of skill in the art, those of skill will appreciate that the following Examples are intended to be exemplary only and that numerous changes, modifications, and alterations can be employed without departing from the scope of the presently disclosed subject matter.

Example 1 Metribuzin Phytoxicity Screen

(2) Soybean seeds were planted two seeds per pot in two inch pots with clay loam soil (1.36 o.m.). Six replications were arranged in a Randomized Complete Block Design in a greenhouse. Metribuzin was applied at 560 grams active ingredient per hectare after planting. Sixteen days later plants were scored for visual phytotoxicity scored as percent injury.

(3) TABLE-US-00002 TABLE 2 Phytotoxocity Scores for Soybean Lines Injury (%) @ 560 g ENTRY LINE ai/ha 1 GO1110272 54 2 AR1111926 20 3 AR1211948 44 4 OW1011073 11 5 OW1113014 34 6 BK1011572 23 7 OW1011829 50 8 WN1118256 47 9 CE1211211 14 10 CE1210923 0 11 WN1116259 0 12 CE1211033 37 13 NE0912640 0 14 SJ1210844 0 15 CC1217018 0 16 CC1211481 12 17 SJ1214029 . 18 G01010146 0 19 AR1211262 0 20 WN1118297 0 21 SJ1114205 0 22 CC1211818 0 23 CC1216972 . 24 BY1212223 0 25 BY1212083 0 26 BY1212201 3 27 A1036478 0 28 AR1112922 0 29 OW1012353 17 30 AR1210886 0 31 AR1210100 15 32 AR1210856 9 33 AR1210104 45 34 GO1110602 53 35 BK1211980 17 36 OW1213207 28 37 OW1213282 23 38 OW1213195 28 39 OW1210742 45 40 OW1213323 42 41 NE1215099 0 42 NE1214412 5 43 NE1212391 0 44 OW1212626 25 45 CE1211264 28 46 CE1213779 38 47 CE1214014 47 48 CE1211403 0 49 CE1211109 0 50 NE1214737 0 51 CE1211693 0 52 CE1211339 0 53 CE1210997 0 54 SJ1210678 0 55 CE1211377 0 56 CE1214602 0 57 CE1212024 0 58 SJ1210829 0 59 SJ1210526 0 60 CE1214443 0 61 CE1214610 47 62 SJ1210862 0 63 SJ1210867 25 64 SJ1210512 11 65 SJ1213014 0 66 SJ1211190 55 67 SJ1210059 0 68 CC1215412 0 69 SJ1213512 0 70 CC1215903 0 71 CC1210319 0 72 SJ1212283 0 73 CC1212005 35 74 CC1215927 45 75 CC1211845 . 76 CC1211717 . 77 CC1211979 . 78 BY1213080 0 79 BY1211100 49 80 BY1210428 54 81 SJ1213805 48 82 CC1217019 0 83 SJ1212410 . 84 CC1211754 . 85 CC1211486 . 86 S77-T7 23 87 S78-G6 10 88 LL511 92 89 LL513N 2 90 LL563N 5 91 LL595N 96

Example 2. QTL Analysis

(4) Genotypes and phytotoxicity phenotypes were analyzed by JMP V12 from SAS, single marker ANOVA and haplotype ANOVA. A QTL for metribuzin tolerance was identified on Linkage Group N.

(5) TABLE-US-00003 TABLE 3 QTL Analysis Results Unfavor- Pheno- Linkage Genetic Marker Favorable able typic QTL Group Position Name Allele Allele Variation QTL N 193.1 SY0670A GG AA 28% # 1 N 225.2 SY0903A AA GG 35%

(6) The above examples clearly illustrate the advantages of the invention. Although the present invention has been described with reference to specific details of certain embodiments thereof, it is not intended that such details should be regarded as limitations upon the scope of the invention except as and to the extent that they are included in the accompanying claims.

(7) Throughout this application, various patents, patent publications and non-patent publications are referenced. The disclosures of these patents, patent publications and non-patent publications in their entireties are incorporated by reference herein into this application in order to more fully describe the state of the art to which this invention pertains.