<i>Cannabis </i>plant named ‘UNIQUE FLOWER ORIGINAL HAZE’

Abstract

The present invention provides a new and distinct Cannabis sativa cultivar designated as ‘UNIQUE FLOWER ORIGINAL HAZE’. This new cultivar is characterized by a persistent green phenotype, extreme resin production, flowering at 14-16 weeks, and significant resistance to pests and fungal growth. ‘UNIQUE FLOWER ORIGINAL HAZE’ is characterized by high levels of Myrcene and Ocimene 2, moderate levels of cis-β Farnesene and β Caryophyllene, and lower levels of additional terpenes including pinenes, α-humulene, linalool, and limonine. ‘UNIQUE FLOWER ORIGINAL HAZE’ has on average 17.38% Total THC and 0.1% Total CBD by dry weight.

Claims

1. A new and distinct Cannabis sativa plant named ‘UNIQUE FLOWER ORIGINAL HAZE’, substantially as described and illustrated herein.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) The accompanying color photographs depict characteristics of ‘UNIQUE FLOWER ORIGINAL HAZE’. The colors shown are as true as can be reasonably obtained by digital photographic procedures and have not been enhanced or adjusted in any way.

(2) FIG. 1 shows the growth form of ‘UNIQUE FLOWER ORIGINAL HAZE’ after transplantation from the greenhouse to a field plot at facilities in Rainier, Oreg.

(3) FIG. 2 shows stem and leaf structure of ‘UNIQUE FLOWER ORIGINAL HAZE’.

(4) FIG. 3 shows a close-up of the leaves of ‘UNIQUE FLOWER ORIGINAL HAZE’.

(5) FIG. 4 shows whole plant clones of ‘UNIQUE FLOWER ORIGINAL HAZE’, including clone #776 from mother plant #7 (‘UNIQUE FLOWER ORIGINAL HAZE’) at 2 weeks after cutting.

(6) FIG. 5 shows whole plant clones of ‘UNIQUE FLOWER ORIGINAL HAZE’ a few weeks after cutting.

(7) FIG. 6 shows a close-up view of a relatively young inflorescence of a clone of ‘UNIQUE FLOWER ORIGINAL HAZE’ at 15 weeks after flower initiation.

(8) FIG. 7 shows a close-up view of a relatively mature inflorescence of a clone of ‘UNIQUE FLOWER ORIGINAL HAZE’ at 15 weeks after flower initiation.

(9) FIG. 8 shows a close-up view of another relatively mature inflorescence of a clone of ‘UNIQUE FLOWER ORIGINAL HAZE’ at 15 weeks after flower initiation.

(10) FIG. 9 shows a more close-up view of an inflorescence of a clone of ‘UNIQUE FLOWER ORIGINAL HAZE’ at 15 weeks after flower initiation, showing dense trichomes.

(11) FIG. 10 shows a more close-up view of another inflorescence of a clone of ‘UNIQUE FLOWER ORIGINAL HAZE’ at 15 weeks after flower initiation, showing dense trichomes.

CHEMICAL CONSTITUENTS

(12) Tables 2 and 3(a), shown below, provide detailed information on the concentrations of cannabinoids and terpenes, respectively, present in the inflorescences of asexual clones of ‘UNIQUE FLOWER ORIGINAL HAZE’ grown and harvested in Rainier, Oreg.

(13) Measurements of cannabinoid analytes for ‘UNIQUE FLOWER ORIGINAL HAZE’ are listed in Table 2, below, based on random sampling from 111.2 g of material from four clones. Sampling and testing was performed and certified by Confident Cannabis, 5691 SE International Way, Portland, Oreg. (www.confidentcannabis.com) using CH SOP 4400 methods. Total THC (Calculated Decarboxylated Potential), determined as (THCa*0.877+d9 THC), was 17.38%. Total CBD (Calculated Decarboxylated Potential), determined as (CBDa*0.877+CDB), was 0.10%. For each analyte in Table 2, the limit of quantification (LOQ) was 0.5 mg/g, and an entry “<LOQ” indicates a measurement below this limit of quantification.

(14) TABLE-US-00002 TABLE 2 Cannabinoids Analyte Mass CY Mass (mgt) THCa 17.77 177.7 Δ.sub.9-THC 1.79 17.9 THCV <LOQ <LOQ CBDa 0.12 1.2 CBD <LOQ <LOQ CBDV <LOQ <LOQ CBN <LOQ <LOQ CBGa 1.20 12.0 CBG 0.06 0.6 CBC 0.08 0.8 Total 21.01 210.1

(15) Measurements of terpene analytes for ‘UNIQUE FLOWER ORIGINAL HAZE’ are listed in Tables 3(a) and 3(b), below, based on random sampling from 111.2 g of material from four clones. Sampling and testing was performed and certified by Confident Cannabis, 5691 SE International Way, Portland, Oreg. (www.confidentcannabis.com) using GC-FID CH SOP 401 methods. For each analyte in Tables 3(a) and 3(b), the limit of quantification (LOQ) was 0.02%.

(16) TABLE-US-00003 TABLE 3(a) Terpenes Detected Analyte Mass (%) Mass (mg/g) β-Myrcene 0.84 8.4 Ocimene 2 0.51 5.1 cis-B-Farnesene 0.26 2.6 β-Caryophyllene 0.20 2.0 α-Pinene 0.12 1.2 α-Humulene 0.09 .9 Linalool 0.06 .6 trans-Phytol 0.05 .5 Limonene 0.04 .4 Terpinolene 0.03 .3 (−)-β-Pinene 0.03 .3

(17) As indicated in Table 3(a), ‘UNIQUE FLOWER ORIGINAL HAZE’ has 0.84% β-Myrcene. Myrcene has been shown to have anti-neuropathic pain effects, and to cause intracellular calcium influxes by acting on nociceptive Transient Receptor Potential V1 receptors (Jansen, C., et al., Channels, 13:1, 344-366 (2019); www.tandfonline.com/doi/full/10.1080/19336950.2019.1654347). In this way, Myrcene may help facilitate the interaction of Anandamide (AEA)—known as the “Bliss molecule” because it helps to modulate brain reward circuitry—with TRPV1 (Scherma, M., et al., Acta Pharmacologica Sinica, 40:309-323 (2019); www.ncbi.nlm.nih.gov/pmc/articles/PMC6460372/). Myrcene also reduces inflammation by inhibiting IL-1β induced responses such as iNOS production (Rufino, A. T., et al., European J. Pharm., 750:141-150 (2015), rihuc.huc.min-saude.pt/bitstream/10400.4/1789/1/alexandrina%202015.pdf).

(18) As indicated in Table 3(a), ‘UNIQUE FLOWER ORIGINAL HAZE’ has 0.51% Ocimene 2, also known as β-Ocimene, and 0.26% cis-β-Farnesene. β-Ocimene is produced by both flowers and vegetative plant tissues, and may attract enemies of phytophagous insects, thereby reducing herbivory (Farré-Armengol, G., et al., Molecules 22:1148 (2017); www.ncbi.nlm.nih.gov/pmc/articles/PMC6152128/). β-Ocimene may also induce pest resistance, for example, resistance to aphids (Cascone, P., et al., J. Plant Physiology, 173:28-32 (2015); www.sciencedirect.com/science/article/abs/pii/S0176161714002296?via%3Dihub). In addition, both β-Ocimene and cis-β-Farnesene have acetylcholinesterase (AChE) inhibitory activity (Wojtunik-Kulesza, K. A., et al., Open Chem, 15:332-343 (2017); www.degruyter.com/document/doi/10.1515/chem-2017-0040/html). AChE is an enzyme that breaks down acetylcholine, a neurotransmitter, so these terpenes may support nerve function.

(19) As indicated in Table 3(a), ‘UNIQUE FLOWER ORIGINAL HAZE’ has 0.20% betaCaryophyllene (BCP) and 0.06% linalool. β-Caryophyllene (BCP) binds directly to CB2 receptors and is a known anti-inflammatory agent with potential for treatment of neurogenic pain. (Johnson, S. A., et al., Hindawi, Article ID 8035301 (2020); www.hindawi.com/journals/ecam/2020/8035301/). Linalool is known to have anxiolytic and antidepressant-like effects. (S. Koyama, et al., Int. J. Mol. Sci., 21:1558 (2020); pubmed.ncbi.nlm.nih.gov/32106479/; Guzmán-Gutiérrez, S. L., et al., Life Sciences, 128:24-29 (2015); www.sciencedirect.com/science/article/abs/pii/S0024320515001381). Linalool is the primary constituent of lavender essential oil, which also contains BCP, and lavender oil has been shown to inhibit fatty acid amide hydrolase (FAAH). (Johnson, 2020.) FAAH is known to hydrolyze AEA, and such inactivation of FAAH may modulate the endocannabinoid system fostering analgesic, anti-inflammatory, anxiolytic and antidepressant effects (Ahn, K., et al., Expert Opin. Drug. Discov. 4(7):763-784 (2009); pubmed.ncbi.nlm.nih.gov/20544003/; Griebel, G., et al., Scientific Reports, 8:2416 (2018); pubmed.ncbi.nlm.nih.gov/29403000/).

(20) As indicated in Table 3(a), ‘UNIQUE FLOWER ORIGINAL HAZE’ has 0.12% α-Pinene and 0.03% (−)-β-Pinene. It is known that α-pinene protects against inflammation and neuronal damage. (Salehi, B, et al., Biomolecules, 9:738 (2019); www.ncbi.nlm.nih.gov/pmc/articles/PMC6920849/.) Furthermore, α-pinene has been shown to increase the response of gamma-aminobutyric-acid (GABA) receptors, allowing nerve cells to better send and receive chemical messages (Yang, H., et al., Mol Pharmacol, 90:530-539 (2016); pubmed.ncbi.nlm.nih.gov/27573669/). Beta-Pinene has been shown to have antidepressant effects and interacts with dopamine receptors in the brain (Guzmán-Gutiérrez 2015).

(21) As indicated in Table 3(a), ‘UNIQUE FLOWER ORIGINAL HAZE’ has 0.09% α-humulene and 0.04% limonene. Alpha-humulene has marked anti-inflammatory properties on the airway's allergic inflammation and is known to reduce eosinophil influx into lung tissue (Rogerio, A. P., et al., Btritish J. Pharmacology, 158:1074-87 (2009); www.ncbi.nlm.nih.gov/pmc/articles/PMC2785529/). Limonene has been shown to prevent bronchial obstruction and reduce peribronchial inflammatory cell infiltration when inhaled (Hirota, R., et al., Inhalation Toxicology, 24(6):373-381 (2012); www.si.mahidol.ac.th/th/department//admin/news_files/30_17_1.pdf).

(22) It is also notable that numerous other terpenes were not present in ‘UNIQUE FLOWER ORIGINAL HAZE’. For each analyte identified in Table 2(b), any measurement of the analyte in ‘UNIQUE FLOWER ORIGINAL HAZE’ was below the limit of quantification.

(23) TABLE-US-00004 TABLE 3(b) Terpenes Not Detected at LOQ = 0.02% Analyte Analyte Analyte Cis-Phytol α-Terpineol Nerol Valencene Cedrol Borneol Sabinene Citral Hexahydro Thymol Ocimene 1 (−)-Guaiol Squalene Geraniol Neryl Acetate δ-3-Carene Neral γ-Terpinene Eucalyptol α-Terpinene Anisole Eugenol Caryophyllene Oxide Fenchone (−)-Isopulegol Camphor Isoborneol Pulegone (−)-α-Bisabolol Camphene Cis-Nerolidol α-Cedrene α-Phellandrene β-Farnesene Endo-Fenchyl Alcohol γ-Terpineol cis-β-Farnesene p-isopropyltoluene Geranyl Acetate α-Famesene Azulene Sabinene Hydrate

DETAILED BOTANICAL DESCRIPTION

(24) The following is a detailed botanical description of the new cultivar described herein, designated ‘UNIQUE FLOWER ORIGINAL HAZE’. The following description is for plants that were approximately 15 weeks post-initiation of flowering at the time of the measurements, and that were grown in an indoor facility and in a field plot at Rainier, Oreg. ‘UNIQUE FLOWER ORIGINAL HAZE’ has not been observed under all possible environmental conditions, and phenotypic differences may be observed with variations in environment without any variance in genotype. The color indications provided herein are based on Munsell book of color, available at munsell.com/. The plant: Type (life form and habit).—A vigorous herbaceous, dioecious, tap-rooted annual plant. Classification.—Cannabis sativa, ssp. sativa. Origin.—Progeny of crosses between Highland Columbian Gold, Punto Rojo, and Santa Marta Columbian Gold. Propagation.—The strain is asexually propagated by stem cutting and cloning. Mature habit.—Extensive fibrous root system with upright and much branched aerial portion of plant having Christmas-tree-like (cone) shape. The growth form of all cloned individuals (n>200) was highly manipulated by systematic removal of terminal buds, inducing a greater branching habit. Overall size in this form varies from 1.6-1.9 m tall and 0.3-0.4 m across at their widest point. Foliage: Leaf arrangement.—Alternate. Form.—Palmately compound/linear-lanceolate leaflets, 5-11 cm. During peak vegetative growth plant will express up to 11 palmately compound leaflets. During flowering leaflet number decreases to singular linear-lanceolate leaflets. Leaf size (with petiole).—8-20 cm long; middle (largest) leaflet 12-14 cm long, 1 cm wide. Leaf margin.—Dentate, coarsely serrated, and the teeth point towards the tip. Leaf color.—Upper side — 7.5GY 6/10; Lower side — 7.5GY 9/12. Veins.—Obliquely continuous throughout leaflet; 7.5Y 9/10. Petiole.—2.5-3 cm long; 5GY 8/8. Stipules.—Acute-bulbous. 1 cm long, 10GY 6/10. Aroma.—Very strong; smells of fresh/sharp vegetable and herbs, pepper, grains of paradise. Stem: Shape.—Hollow, punctuate, minutely glandular, ribbed with ribs running parallel to stem. Size.—3.5-6 cm diameter at base. Color.—5GY 8/8 striped with 7.5R 4/14 segments in late flower. Internode length.—3-12 cm. Breaking action.—Extremely flexible, resistant to breakage. Inflorescence: Blooming/flowering habit.—Cymes from 0.5-2.2 m in length. Elongated compound cymes or panicles, forming spikes from 0.3-0.5 m in length, loosely packed with individual small pistillate flowers subtended by small leaves, these with densely packed capitate glandular trichomes. Number of flowers.—50-150 per cyme. Flowers: Shape.—Calcarate-urceolate; a small green bract enclosing the ovary with two long, slender stigmas projecting well above the bract. Open floral structure with stacked bracts along stems that form flowers in fox tail formations. Flower (individual pistillate) length.—7 mm. Bract.—Urceolate, 0.3-0.5 cm, 10GY 6/10. Stigma.—Linear-lanceolate, 5-7 mm, 5GY 9/6 while immature, 10R 5/12 when mature. Trichome.— Capitate sessile trichomes (color: clear when immature at day 40 in flowering) are present on the leaves of plants, as well as being noticed in the flowers; During later flowering (day 98 to day 112 in flowering), capitate stalked trichomes (color: N9 at peek maturity) are present. Bulbous and non-glandular trichomes (color: N9) are also present and most noticeable on the petioles. Cola (terminal bud).—Elliptical, 10GY 6/10. Pedicel.—Absent. Pollen.—Absent. Petal.—Apetalous; this part is fused and appressed to the base of the ovary with the calyx and the perianth in the Cannabis flowers. Petal arrangement.—Free. General characteristics: Time to first flowering.—4-6 weeks. Flowering/blooming period.—14-16 weeks. Flowering stimulus.—Decreasing light to less than 12 hours per day. Hardiness.—Hardy to 35° F. ambient temperature. Rooting rate after cutting/cloning.—99% vigorous. Pest susceptibility.—Resistant to pests including spider mites, hemp russet mites, aphids, and white flies. Disease susceptibility.—Resistant to diseases such as bud rot or grey mold (Botrytis cinerea), Fusarium wilt (Fusarium oxysporum). Storage life.—Long: 3-8 months with minor changes in physical appearance and/or smell/taste; potentially years if stored in airtight container in a dark and cool location. Productivity of flower.—Approximately 0.23-0.9 kg can be produced per plant, depending on finished plant size (1.0-4.0 m); approximately 400-800 grams can be produced per plant at an average size of 1.3 m×0.7 m. Market use.—Medicinal and recreational.