Crystalline pharmaceutical co-crystals of glycopyrronium bromide with lactose
10662152 ยท 2020-05-26
Assignee
Inventors
Cpc classification
A61P29/00
HUMAN NECESSITIES
International classification
Abstract
The present invention provides co-crystals of glycopyrronium bromide with lactose. The glycopyrronium bromide and lactose in the novel co-crystals are present in a stoichiometric ratio of from about 1:2 to 2:1. These are characterized by XRD and DSC. Processes for preparing the novel co-crystals are also provided. The co-crystals are also disclosed for use as a medicament, in particular, for treatment of respiratory complaints, such as chronic pulmonary obstructive disease (COPD), bronchitis and asthma. Pharmaceutical compositions comprising the co-crystals as active ingredient are also presented.
Claims
1. A co-crystal of glycopyrronium bromide comprising glycopyrronium bromide and lactose, wherein the glycopyrronium bromide and lactose are present in a stoichiometric ratio of from about 1:2 to 2:1.
2. The co-crystal according to claim 1, wherein the lactose is selected from monohydrate lactose, anhydrous lactose or amorphous lactose.
3. The co-crystal according to claim 1, wherein the lactose is a crystalline lactose monohydrate.
4. The co-crystal according to claim 1, wherein the stoichiometric ratio of glycopyrronium bromide and lactose in the co-crystal is 1:1.
5. The co-crystal according to claim 1, wherein the stoichiometric ratio of glycopyrronium bromide and lactose in the co-crystal is 1:2.
6. The co-crystal according to claim 1, wherein the stoichiometric ratio of glycopyrronium bromide and lactose in the co-crystal is 2:1.
7. The co-crystal according to claim 1, characterized by X-Ray spectrum with characteristic 2theta values at: 5.50; 9.12; 9.78; 10.84; 12.48; 1.3.7; 14.28; 14.38; 15.80; 1.6.18; 16.90; 18.16; 18.62; 19.36; 19.40; 19.54; 20.06; 21.06; 21.56; 21.92; 22.72; 23.38; 24.34; 24.64; 25.14; 25.76; 2620; 27.02; 27.34; 28.52; 29.50; 29.98; 30.76; 32.52; 33.28; 34.64; 36.10; 37.06; 38.08; 39.18; 42.66 43.26; and 45.38; 0.2 2.
8. The co-crystal according to claim 1, characterized by X-Ray spectrum with characteristic 2theta values at: 5.58; 10.92; 12.1; 12.76; 1.4.44; 14.48; 15.92; 16.28; 15.92; 16.62; 17.32; 18.22; 18.68; 19.4; 1.9.82; 20.22; 21.1; 21.62; 21.64; 22.08; 23.02; 23.42; 23.96; 24.44; 24.72; 25.32; 25.86; 26.34; 27.08; 27.46; 27.64; 28.62; 30.08; 30.38; 30.86; 31.28; 31.68; 31.92; 32.58; 32.90; 3430; 34.74; 3514; 35.50; 35.86; 36.44; 37.16; 37.80; 38.42; 38.74; 0.2 2.
9. The co crystal according to claim 1, characterized by X-Ray spectrum with characteristic 2theta values at: 5.44; 10.76; 12.48; 14.34; 15.76; 16.10; 16.48; 16.84; 18.56; 19.28; 19.98; 21.48; 21.88; 22.66; 23.36; 24.16; 24.28; 24.60; 25.08; 25.14; 25.72; 26.18; 26.94; 27.36; 28.48; 29.46; 29.94; 30.24; 30.72; 31.70; 32.12; 32.76; 33.24; 33.54; 34.12; 34.60; 35.36; 36.18; 37.06; 38.00; 38.24; 39.12; 40.24; 40.76; 41.36; 42.00; 42.58; 43.24; 43.94; 44.82; 45.36; 46.24; 46.70; 47.76; 0.2 2.
10. The co-crystal according to claim 1, characterized by a singe endothermic event at about 172 C. determined by DSC.
11. The co-crystal according to claim 1, wherein the co-crystal is in a micronized form.
12. The co-crystal according to claim 11, wherein the micronized co-crystal has a particle size in the range of about 2 microns to about 5 microns.
13. A method comprising administering a medicament comprising the co-crystal according to claim 1 to a subject in need thereof for the treatment of chronic pulmonary obstructive disease (COPD), bronchitis, asthma or hyperhidrosis.
14. A process for producing a co-crystal of glycopyrronium bromide comprising glycopyrronium bromide and lactose present in a stoichiometric ratio of from about 1:2 to 2:1, the process comprising the steps of: (a) mixing glycopyrronium bromide and lactose in a solvent to form a reaction mixture; (b) heating the reaction mixture to obtain a solution; (c) adding an anti-solvent to the reaction mixture; (d) cooling the reaction mixture, under stirring; (e) filtering or spray drying the product obtained after cooling; and (f) drying the product if required to obtain co-crystals of glycopyrronium bromide and lactose.
15. The process according to claim 14 wherein in step a) the glycopyrronium bromide and lactose are dissolved in a relative molar ratio of from 0.5 to 2.2.
16. The process according to claim 14, wherein in step f) the product is dried under vacuum at a temperature of from about 35 C. to about 40 C.
17. The process according to claim 14, wherein the product in step e) is washed using a solvent such as ethyl acetate or methyl ethyl ketone (MEK) or mixture thereof.
18. The process according to claim 14, wherein the lactose is selected from monohydrate lactose, anhydrous lactose or amorphous lactose.
19. The process according to claim 14, wherein the solvent is selected from dimethylsulfoxide (DMSO), dimethylformamide (DMF), dimethylacetamide (DMA) or mixtures of one or more thereof.
20. The process according to claim 14, wherein the anti-solvent is selected from aprotic solvent, ketones, esters, ethers, acetonitrile, acetone ethyl acetate or methyl ethyl ketone (MEK) or mixtures of one or more thereof.
21. The process according to claim 14, wherein the co-crystals of glycopyrronium bromide and lactose from step f) is micronized.
22. The process according to claim 21, wherein the micronized co-crystals of glycopyrronium bromide and lactose have a particle size of about 2 microns to about 5 microns.
23. The process according to claim 14, wherein in step h) the reaction mixture is heated to a temperature of from 50 C. to 55 C. to form a solution.
24. The process according to claim 14, wherein in step d) the reaction mixture is cooled to a temperature of from about 2.0 C. to about 25 C., and the reaction mixture is kept at this temperature under stirring for about 3 hours to obtain a precipitate.
25. A pharmaceutical formulation comprising co-crystals of glycopyrronium bromide and lactose according to claim 1 and one or more pharmaceutically acceptable excipients.
26. A method comprising administering a pharmaceutical composition comprising the co-crystals glycopyrronium bromide and lactose according to claim 1 to a subject in need thereof for the treatment of chronic pulmonary obstructive disease (COPD), bronchitis or asthma.
27. The process according to claim 14, wherein the lactose is a crystalline lactose monohydrate.
28. The pharmaceutical formulation according to claim 25, wherein the pharmaceutically acceptable excipient comprises one or more surfactants.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
(1)
(2)
DETAILED DESCRIPTION OF THE INVENTION
(3) The present invention describes the production of a crystal modification of glycopyrronium bromide that can be obtained in the form of a co-crystal with lactose.
(4) The novel co-crystal of glycopyrronium bromide and lactose of the present invention has several advantages including, but not limited to, increase in dissolution rate without compromising the thermodynamic stability, and improved properties in relation to physical stability and bioavailability.
(5) The present invention also describes a pharmaceutical formulation comprising the novel co-crystal of glycopyrronium bromide and lactose.
(6) Co-crystals are being studied intensively primarily due to the potential for improved pharmaceutical properties, including bioavailability, dissolution rate, hygroscopicity, physical stability, purification processability, compressibility, flowability and shelf-life.
(7) In the case of designing co-crystals of marketed drugs, clinical trials program will be significantly shorter and less risky than those of New Chemical Entities (NCEs) since co-crystals do not involve structural modification of the parent molecules.
(8) Co-crystals are solids that are crystalline materials composed of two or more molecules in the same crystal lattice where each component is defined as either an atom, ion, or molecule and do not involve structural modification of the parent molecule. In contrast to salts, the intermolecular interactions are not ionic, but weak forces like hydrogen bonding, stacking and Van der Waals forces.
(9) Due to the higher complexity of their crystal structure, co-crystals are also less prone to suffer polymorphic transformations. API in co-crystals will not form solvates, hydrates during crystallization or storage.
(10) The European Medicines Agency defines co-crystals as: co-crystals are crystalline structures made up of two or more components in a definitive stoichiometric ratio where the arrangement in the crystal lattice is not based on ionic bonds.
(11) Co-crystals of glycopyrronium bromide and lactose of the present invention can be prepared by various methods including, but not limited to, anti-solvent addition, microfluidization technology, high pressure homogenization (HPH), and spray congealing.
(12) Co-crystals consist of multiple components in given stoichiometric ratio, where different molecular species interact by hydrogen bonding and by non-hydrogen bonding.
(13) Co-crystals of glycopyrronium bromide and lactose of the present invention, have various ratios of glycopyrronium bromide and lactose. Preferably, the co-crystals of the present invention have a stoichiometric ratio in the range of from 1:2 to 2:1. The stoichiometry ratio refers to the ratio of molecules in the unit cell of the co-crystals. The stoichiometry ratio can be determined by known analytical technique. In the present invention, the stoichiometric ratio is calculated based on the amounts of actives used in the preparation of the co-crystals and analysis of the final product indicates that no free lactose is detected.
(14) Method 1Anti-Solvent Addition
(15) Co-crystals of glycopyrronium bromide and lactose of the present invention may be obtained by:
(16) (a) Mixing glycopyrronium bromide and lactose, preferably in a relative molar ratio, in the range of from 0.5 to 2.2, preferably in the range of from 1.0 to 2.2 or from 0.5 to 1.8, in a solvent to form a reaction mixture. The solvent is preferably selected from dimethylsulfoxide (DMSO), dimethylformamide (DMF), dimethylacetamide (DMA) or mixtures of one or more thereof.
(b) Heating the reaction mixture until a solution is obtained. Preferably, the reaction mixture is heated to a temperature of from about 40 C. to about 55 C., more preferably about 50 C. to about 55 C. to form a solution.
(c) Adding slowly to the reaction mixture, under stirring, an anti-solvent preferably an aprotic solvent. Suitable anti-solvents also includes, but not limited to, ketones, esters, ethers acetonitrile, ethyl acetate, acetone, methyl ethyl ketone (MEK) or mixtures of one or more and mixtures thereof.
(d) Cooling the reaction mixture under stirring.
(e) Filtering the product or spray drying the product; and
(f) Suitably drying under vacuum after filtering the product to obtain co-crystals of glycopyrronium bromide and lactose. If the product is spray dried then it may not be necessary to further dry the product.
(17) In step (c) above, the anti-solvent may be added dropwise to the reaction mixture and the reaction mixture is kept at a temperature of from 50 C. to 55 C. to obtain a suspension.
(18) In step (d) above, the reaction mixture may be cooled to a temperature of from about 20 C. to about 25 C. and the reaction mixture is kept at the temperature range of from about 20 C. to about 25 C. under stirring for 3 hours. After filtering, the product obtained in step (e) may be washed using a solvent preferably ethyl acetate or methyl ethyl ketone (MEK). In step d) above, the product may be dried under vacuum at a temperature of from about 35 C. to about 40 C. The lactose used in the process according to the present invention can be crystalline monohydrate lactose, anhydrous lactose or amorphous lactose, preferably crystalline lactose monohydrate.
(19) The co-crystals obtained by the process of the present invention were analyzed by X-Ray powder diffraction (XRPD), and differential scanning calorimetry (DSC). The analysis showed that a new crystalline form, a co-crystal of glycopyrronium bromide and lactose is formed.
(20) For XRPD, data collection was carried out at room temperature using monochromatic CuK radiation in the 20 region between 1.5 and 41.5, which is the most distinctive part of the XRPD pattern. The diffraction pattern of each well was collected in two 20 ranges (1.5221.5 for the first frame, and 19.50241.5 for the second) with an exposure time of 45 s for each frame. No background subtraction or curve smoothing was applied to the XRPD patterns.
(21) For the present invention, melting properties were obtained from DSC thermograms, recorded with a heat flux DSC822e instructment (Mettler-Toledo GmbH, Switzerland). The DSC822e was calibrated for temperature and enthalpy with a small piece of indium (m.p.=156.6 C.; Hf=28.45 J/g). Samples (circa 2 mg) were sealed in standard 40 l aluminium pans, pin-holed and heated in the DSC from 25 C. to 300 C., at a heating rate of 10 C./min. Dry N.sub.2 gas, at a flow rate of 50 mL/min was used to purge the DSC equipment during measurement.
(22) For the present invention, mass loss due to solvent or water loss from the crystals was determined by TGA/SDTA. Monitoring the sample weight, during heating in a TGA/SDTA851e instrument (Mettler-Toledo GmbH, Switzerland), resulted in a weight vs. temperature curve. The TGA/SDTA851e was calibrated for temperature with indium and aluminium. Samples were weighed into 100 l aluminium crucibles (Mettler Toledo) and sealed. The lids were pin-holed right before the measurement and the crucibles heated in the TGA from 25 to 300 C. at a heating rate of 10 C. min.sup.1. Dry N.sub.2 gas was used for purging.
(23) The analysis indicated that co-crystals of glycopyrronium bromide and lactose with a stoichiometric ratio of 1:1 has a single endothermic event at about 172 C. determined by DSC and a characteristic diffraction peaks presented below:
(24) TABLE-US-00001 2theta I/I.sub.0 5.50 64 9.12 3 9.78 3 10.84 66 12.48 5 13.7 4 14.28 10 14.38 10 15.80 7 16.18 15 16.90 4 18.16 11 18.62 13 19.36 18 19.40 18 19.54 17 20.06 10 21.06 10 21.56 100 21.92 8 22.72 5 23.38 8 24.34 9 24.64 11 25.14 7 25.76 6 26.20 7 27.02 20 27.34 10 28.52 11 29.50 10 29.98 6 30.76 9 32.52 7 33.28 5 34.64 9 36.10 6 37.06 5 38.08 8 39.18 5 42.66 5 43.26 6 45.38 6
(25) The analysis also indicated that characteristic diffraction peaks of co-crystals of glycopyrronium bromide and lactose with a stoichiometric ratio of 1:2 may comprise one or more peaks presented below, and has a single endothermic event at about 172 C. determined by DSC:
(26) TABLE-US-00002 2theta I/I.sub.0 5.58 44 10.92 53 12.1 11 12.76 32 14.44 19 14.48 19 15.92 16 16.28 18 15.92 16 16.62 25 17.32 10 18.22 14 18.68 20 19.4 48 19.82 40 20.22 76 21.1 22 21.62 100 21.64 99 22.08 16 23.02 16 23.42 13 23.96 26 24.44 22 24.72 20 25.32 16 25.86 21 26.34 15 27.08 26 27.46 16 27.64 16 28.62 21 30.08 13 30.38 11 30.86 21 31.28 12 31.68 11 31.92 11 32.58 10 32.90 11 34.30 14 34.74 20 35.14 14 35.50 10 35.86 11 36.44 19 37.16 19 37.80 16 38.42 20 38.74 13
(27) The analysis also indicated that characteristic diffraction peaks of co-crystals of glycopyrronium bromide and lactose with a stoichiometric ratio of 2:1 may comprise one or more peaks presented below, and has a single endothermic event at about 172 C. determined by DSC:
(28) TABLE-US-00003 2theta I/I.sub.0 5.44 50 10.76 55 12.48 9 14.34 35 15.76 27 16.10 18 16.48 9 16.84 9 18.56 34 19.28 48 19.98 36 21.48 100 21.88 21 22.66 11 23.36 14 24.16 23 24.28 34 24.60 28 25.08 20 25.14 21 25.72 17 26.18 15 26.94 25 27.36 18 28.48 24 29.46 23 29.94 16 30.24 13 30.72 31 31.70 10 32.12 12 32.76 13 33.24 12 33.54 10 34.12 12 34.60 18 35.36 9 36.18 14 37.06 11 38.00 13 38.24 13 39.12 11 40.24 9 40.76 11 41.36 9 42.00 10 42.58 10 43.24 13 43.94 10 44.82 9 45.36 13 46.24 10 46.70 9 47.76 10
Method 2Co-Crystallization Using Co-Precipitation by Micro-Fluidization or Micro-Reaction
(29) Co-crystals of glycopyrronium bromide and lactose of the present invention may be obtained by using microreactor or microstructured reactor or microchannel reactor. A wide range of microreactors and microfluidizer are known in the art. Any known microreactor or microstructured reactor or microchannel reactor may be used in this method.
(30) In the present invention, a microfluidizer processor (Microfluidics, Model MRT CR5) comprising a chamber with 75 m diameter reaction channels followed by an auxiliary processing module with 200 m diameter reaction channels was used.
(31) The method comprises the following steps:
(32) 1. Dissolving the API (glycopyrronium bromide) and lactose in an appropriate solvent and placing the solution in a first inlet reservoir. The solvent is preferably selected from dimethylsulfoxide (DMSO), dimethylformamide (DMF), dimethylacetamide (DMA) or mixtures of one or more thereof.
2. Placing an anti-solvent in a second inlet reservoir. Preferably, the anti-solvent is selected from aprotic solvent, ketones, esters, ethers, acetone, acetonitrile, ethyl acetate or methyl ethyl ketone (MEK) and mixtures thereof.
3. Both the solution from step (1) and the anti-solvent from step (2) are pressurized in a combined stream via one or more intensifier pumps to the micro-reactor. The intensifier pump is preferably set to impose a pressure of 20 kPsi. A ratio of 1:2 of solvent and anti-solvent is maintained preferably by using a peristaltic pump which is set to impose the desired ratio.
4. Both solutions interact within the micro-reactor at a nano-scale level to form a suspension of particles by co-precipitation to form a co-crystal of glycopyrronium bromide and lactose.
Method 3Co-Crystallization Method Via Particle Size Reduction Through Wet Milling
(33) Co-crystals of glycopyrronium bromide and lactose of the present invention may be obtained by using microreactor or microstructured reactor or microchannel reactor. Any known microreactor or microstructured reactor or microchannel reactor may be used in this method.
(34) In the present invention, a microfluidizer processor (Microfluidics, Model MRT CR5) comprising a chamber with 75 m diameter reaction channels followed by an auxiliary processing module with 200 m diameter reaction channels was used.
(35) Co-crystals of glycopyrronium bromide and lactose of the present invention may be obtained by:
(36) 1. Suspending the API (glycopyrronium bromide) and lactose in an appropriate anti-solvent and placing the suspension in an inlet reservoir. Preferably, the anti-solvent is selected from aprotic solvent, ketones, esters, ethers acetone, acetonitrile ethyl acetate, methyl ethyl ketone (MEK), and mixtures thereof
2. The suspension is pressurized via one or more intensifier pumps to the microchannel(s)
3. The suspension returns to the inlet reservoir being re-pressurized via one or more intensifier pumps to the microchannel(s). The intensifier pump is preferably set to impose a pressure of 20 kPsi. A ratio of 1:2 of solvent and anti-solvent is maintained preferably by using a peristaltic pump which is set to impose the desired ratio.
4. The number of cycles through the microchannel(s) is defined according to the final target co-crystal purity, which may be determined by the skilled person.
Method 4Spray Congealing (SC)
(37) Co-crystals of glycopyrronium bromide and lactose of the present invention may be obtained by the Spray Congealing process described in WO2015036799, which is incorporated by reference in its entirety, comprising the following steps:
(38) a) feeding a molten mixture of glycopyrronium bromide (a first substance) and lactose (a second substance) which are able to form co-crystals to an atomizer;
(39) b) atomizing the molten mixture to droplets;
(40) c) solidifying the droplets to particles; and
(41) d) collecting the particles of co-crystals of glycopyrronium bromide and lactose.
(42) Preferably, the co-crystals of glycopyrronium bromide and lactose obtained by the processes of the present invention are micronized.
(43) The co-crystal according to the invention may be obtained in finely divided form using methods known in the prior art. Known methods of micronizing active substances, such as the air jet mill or the conical screen mill techniques may be used to micronize the co-crystals of glycopyrronium bromide and lactose of the present invention for use in a pharmaceutical composition.
(44) For example, for delivery of pharmaceutical co-crystal of glycopyrronium bromide and lactose by inhalation the particle size may be in the range of from about 2 microns to 5 microns.
(45) The lactose used above mentioned processes can be crystalline monohydrate, anhydrous or amorphous, preferably crystalline lactose monohydrate.
(46) The co-crystals of glycopyrronium bromide and lactose of the present invention can be formulated in a pharmaceutical composition comprising the co-crystals and optionally one or more pharmaceutically acceptable excipients and/or surfactants. Suitable pharmaceutically acceptable excipients include carriers, diluents, wetting agents, emulsifying agents, binders, coatings, fillers, glidants, lubricants, disintegrants, preservatives, surfactants, pH buffering substances and the like known in the art may be used in the formulation.
(47) The pharmaceutical compositions comprising the co-crystals as active ingredient can be used as a medicament, in particular, for treating respiratory complaints, such as chronic pulmonary obstructive disease (COPD), bronchitis and asthma.
(48) The present invention further provides a method for the treatment in a mammal, such as a human, for treating respiratory, inflammatory or obstructive airway disease such as COPD and asthma, which method comprises administration of a therapeutically effective amount of a pharmaceutical composition comprising co-crystal of glycopyrronium bromide and lactose according to the present invention. The method of treatment may be characterized in that co-crystal of glycopyrronium bromide and lactose is administered in therapeutically effective amounts to the patient.
(49) The pharmaceutical compositions may be prepared by admixing co-crystal of glycopyrronium bromide and lactose of the present invention and one or more pharmaceutically acceptable excipients.
(50) The dosage and mode of administration can be decided by the expert of the art, based on the common general knowledge.
(51) The pharmaceutical compositions may be formulated to be delivered by any suitable route, including oral, intravenous, parenteral, inhalation, intranasal, topical, subcutaneous, or intramuscular. Suitable dosage forms include, not limited to tablets, capsules, powders, sustained release formulations, ointments, gels, creams, suppositories, eye drops, transdermal patches, syrups, solutions, suspensions, aerosols, solutions for nebulizers, nasal sprays, etc.
(52) The pharmaceutical compositions of the present invention may be administered by any suitable method used for delivery of drugs to the respiratory tract. The composition of the present invention may thus be administered using metered dose inhalers (MDI), dry powder inhalers (DPI), nebulisers, nasal sprays, nasal drops, insufflation powders, sprays and spray patches.
(53) In a preferred embodiment, the pharmaceutical composition of the present invention is suitable for administration by inhalation or intranasal routes, such as an aerosol solution or suspension, as a dry powder for inhalation, or in a nasal spray. The composition for inhalation comprises co-crystal of glycopyrronium bromide and lactose having particle size. Preferably, the particle size of co-crystal glycopyrronium bromide and lactose can be in the range of from about 2 microns to about 5 microns.
(54) The present invention further provides a method for the treatment in a mammal, such as a human, for treating hyperhidrosis.
EXAMPLES
(55) The following examples are intended to illustrate the invention, without limiting it in any way.
Example 1Preparation of Co-Crystals Glycopyrronium Bromide and Lactose
(56) Glycopyrronium bromide (2.0 g; 5.0 mmol) and lactose monohydrate (1.98 g; 5.49 mmol) were dissolved in dimethylsulfoxide (DMSO) (10 ml) at a temperature of from 50 C. to 55 C. Then ethyl acetate (50 ml) was added dropwise maintaining the reaction mixture at a temperature of from 50 C. to 55 C.
(57) A suspension is obtained and the reaction mixture is cooled down at a temperature of from 20 C. to 25 C. and kept at this temperature under stirring for 3 hours.
(58) The product was filtered and washed with ethyl acetate and then dried under vacuum at a temperature of from 35 C. to 40 C., to yield 3.7 g of co-crystals of glycopyrronium bromide and lactose.
(59) The product was analyzed using Differential Scanning Calorimetry (DSC). The XRPD result indicates that a new crystalline form, a co-crystal of glycopyrronium bromide with lactose is formed.
(60) The XRPD and DSC characterization of the co-crystals is depicted as GBr1 in
Example 2Preparation of Co-Crystals Glycopyrronium Bromide and Lactose
(61) Glycopyrronium bromide (2.0 g; 5.0 mmol) and lactose monohydrate (1.98 g; 5.49 mmol) were dissolved in dimethylformamide (DMF) (10 ml) at a temperature of from 50 C. to 55 C. Then ethyl acetate (50 ml) was added dropwise maintaining the reaction mixture at a temperature of from 50 C. to 55 C.
(62) A suspension is obtained and the reaction mixture is cooled down at a temperature of from 20-25 C. and kept at this temperature under stirring for 3 hours.
(63) The product is filtered and washed with ethyl acetate and then dried under vacuum at a temperature of from 35-40 C. to yield 3.83 g of co-crystals of glycopyrronium bromide and lactose.
(64) The product was analyzed using Differential Scanning Calorimetry (DSC). The XRPD result indicates that a new crystalline form, a co-crystal of glycopyrronium bromide with lactose is formed.
(65) The XRPD and DSC characterization of the co-crystals is depicted as GBr2 in
Example 3Preparation of Co-Crystals Glycopyrronium Bromide and Lactose
(66) Glycopyrronium bromide (2.0 g; 5.0 mmol) and lactose monohydrate (1.98 g; 5.49 mmol) were dissolved in dimethylformamide (DMF) (40 ml) at a temperature of from 50 C. to 55 C. Then methyl ethyl ketone (MEK) (80 ml) was added dropwise maintaining the reaction mixture at a temperature of from 50 C. to 55 C.
(67) A suspension is obtained and the reaction mixture is cooled down at 20-25 C. and kept at this temperature under stirring for 3 hours.
(68) The product is filtered, washed with methyl ethyl ketone (MEK) and then dried under vacuum at a temperature of from 35 C.-40 C. to yield 3.06 g of co-crystals of glycopyrronium bromide and lactose.
(69) The product was analysed using Differential scanning calorimetry (DSC). The XRPD result indicates that a new crystalline form, a co-crystal of glycopyrronium bromide with lactose is formed.
Example 4Preparation of Co-Crystals Glycopyrronium Bromide and Lactose
(70) Glycopyrronium bromide (1.0 g; 2.5 mmol) and lactose monohydrate (1.8 g; 5.0 mmol) were dissolved in dimethylsulphoxide (DMSO) (15 ml) at a temperature of from 50 C. to 55 C. Then ethyl acetate (60 ml) was added dropwise maintaining the reaction mixture at a temperature of from 50 C. to 55 C.
(71) A suspension is obtained and the reaction mixture is cooled down at temperature of from 20 C. to 25 C. and kept at this temperature under stirring for 3 hours.
(72) The product is filtered, washed with ethyl acetate and then dried under vacuum at a temperature of from 25 C.-30 C. to yield 2.5 g of co-crystals of glycopyrronium bromide and lactose.
(73) The product was analyzed using Differential Scanning Calorimetry (DSC). The XRPD result indicates that a new crystalline form, a co-crystal of glycopyrronium bromide with lactose is formed.
Example 5Preparation of Co-Crystals Glycopyrronium Bromide and Lactose
(74) Glycopyrronium bromide (1.0 g; 2.5 mmol) and lactose monohydrate (0.5 g; 1.38 mmol) were dissolved in dimethylsulphoxide (DMSO) (10 ml) at a temperature of from 50 C. to 55 C. Then ethyl acetate (40 ml) was added dropwise maintaining the reaction mixture at a temperature of from 50 C. to 55 C.
(75) A suspension is obtained and the reaction mixture is cooled down at a temperature of from 20 C. to 25 C. and kept at this temperature under stirring for 3 hours.
(76) The product is filtered, washed with ethyl acetate and then dried under vacuum at a temperature of from 25 to 30 C. to yield 0.88 g of co-crystals of glycopyrronium bromide and lactose.
(77) The product was analyzed using Differential scanning calorimetry (DSC). The XRPD result indicates that a new crystalline form, a co-crystal of glycopyrronium bromide with lactose is formed.