Medical device for the observation of a partly fluorescent object, using a filter system with a transmission window

Abstract

The invention relates to a medical device (1) for the observation of a partly fluorescent object (2) such as tissue (3) comprising at least one fluorophore (4). The fluorophore (4) absorbs light in at least one spectral excitation waveband (46) and emits fluorescent light in at least one spectral emission waveband (54). In order to be able to observe also non-fluorescent regions in the tissue (3) without complicated filter arrangement, the medical device (1) according to the invention comprises at least one filter system (16, 38) which comprises, in a filter plane (18), comprises a filter area (20) and a transmission window (22). The filter area (20) comprises a band pass filter (24) having at least one passband (44) comprising the at least one excitation waveband. The transmission window has a passband (48) which is wider than the passband (44) of the filter area (20). In particular, a filter layer (64) of the filter area (20) may be missing in the transmission window (20).

Claims

1. A medical device for the observation of a partly fluorescent object such as tissue comprising at least one fluorophore, the fluorophore absorbing light in at least one spectral excitation waveband and emitting fluorescent light in at least one spectral emission waveband, the medical device comprising: at least one filter system, the filter system being arranged in a filter plane, wherein, in the filter plane, the filter system comprises: a filter area comprising a band pass filter having at least one passband, the passband comprising at least one of the excitation waveband and the emission waveband, and a transmission window having a passband which is wider than the passband of the filter area, wherein the at least one filter system is configured to simultaneously receive light through the filter area and the transmission window, and wherein, in the filter plane, a light flux through the transmission window is less than 1% of a light flux through the filter system.

2. The medical device according to claim 1, wherein at least one of the filter area and the transmission window is comprised of disconnected regions.

3. The medical device according to claim 1, wherein the filter area comprises at least one filter layer which is not present in the transmission window.

4. The medical device according to claim 1, wherein the filter system comprises a light-transmissive substrate on which the band pass filter is arranged as at least one filter layer.

5. The medical device according to claim 1, wherein the transmission window is symmetric about at least one radial direction passing through an optical axis of the medical device at the filter plane.

6. The medical device according to claim 1, wherein the filter system comprises a substrate, the substrate having two opposing surfaces, wherein the filter area is arranged on each of the two opposing surfaces and the transmission area is arranged on each of the two opposing surfaces.

7. The medical device according to claim 6, wherein the filter area on one of the two opposing surfaces is located opposite the transmission window on the other one of the two opposing surfaces.

8. The medical device according to claim 1, wherein the filter system comprises at least one moveable filter element, which is supported moveably with respect to an optical axis, the moveable filter element comprising at least one of the filter area and the transmission window.

9. The medical device according to claim 8, wherein the moveable filter element is a cover mask which is relatively moveable from a first position to a second position and wherein the cover mask in the first position covers a larger part of the transmission window than in the second position.

10. The medical device according to claim 8, wherein the moveable filter element is adapted to be moved from a first position to a second position and wherein, in the first position, the transmission window is smaller than in the second position.

11. The medical device according claim 8, further comprising an actuator system, the actuator system being operatively coupled to the at least one moveable filter element and being adapted to move the at least one moveable filter element from a first position to a second position relative to the optical axis.

12. The medical device according to claim 1, wherein the filter system comprises at least one moveable filter element and at least one stationary filter element, the movable filter element being configured to be moved away from the at least one stationary filter element.

13. The medical device according to claim 1, the medical device further comprising a light source, an observation zone and a viewing system for viewing fluorescent tissue arranged in the observation zone, the light source being configured to emit light in the at least one fluorescence excitation waveband in the direction of the observation zone, wherein the filter system is arranged between at least one of the light source and the observation zone, and the observation zone and the viewing system.

14. The medical device according to claim 1, wherein the at least one passband of the band pass filter comprises a spectral passband which is restricted to one of the fluorescence emission wavebands and the fluorescence excitation wavebands.

15. The medical device according to claim 1, wherein a resulting spectrum of light passed through the at least one filter system includes side bands of the passband of the filter area.

16. A filter system for arrangement in a filter plane of a medical device, the filter system comprising: a filter area comprising a band pass filter having at least one passband, the passband comprising at least one of an excitation waveband and an emission waveband, and a transmission window having a passband which is wider than the passband of the filter area, wherein the filter system is configured to simultaneously receive light through the filter area and the transmission window, and wherein, in the filter plane, a light flux through the transmission window is less than 1% of a light flux through the filter system.

17. The filter system according to claim 16, wherein a resulting spectrum of light passed through the filter system includes side bands of the passband of the filter area.

18. A filter system for arrangement in a filter plane of a medical device, the filter system comprising: a filter area comprising a band pass filter having at least one passband, the passband comprising at least one of an excitation waveband and an emission waveband, and a transmission window having a passband which is wider than the passband of the filter area, wherein the filter system is configured to simultaneously receive light through the filter area and the transmission window, and wherein the filter system further comprises a substrate, the substrate having two opposing surfaces, wherein the filter area is arranged on each of the two opposing surfaces and the transmission area is arranged on each of the two opposing surfaces.

Description

BRIEF DESCRIPTION OF THE DRAWING VIEWS

(1) In the figures:

(2) FIG. 1 shows a schematic representation of a medical device according to the invention;

(3) FIG. 2 shows a schematic representation of the spectral filtering properties of a filter system of the medical system of FIG. 1;

(4) FIG. 3 shows a schematic representation of the spectral filtering properties of a filter system of the medical system of FIG. 1;

(5) FIG. 4 shows a schematic representation of the spectral filtering properties of a filter system of the medical system of FIG. 1;

(6) FIG. 5 shows a schematic representation of a filter system of the medical device of FIG. 4;

(7) FIG. 6 shows a schematic cross-section along the line VI-VI in FIG. 5;

(8) FIG. 7 shows a schematic cut view along the line VI-VI of FIG. 5 together with incident light;

(9) FIG. 8 shows a cut view along the line VI-VI of FIG. 5 of another embodiment;

(10) FIG. 9-11 show schematic representations of various filter systems of the medical device shown in FIG. 1;

(11) FIG. 12 shows a schematic representation of another embodiment of a filter system of the medical device of FIG. 1 in a first position;

(12) FIG. 13 shows the filter system of FIG. 12 in a second position;

(13) FIG. 14 shows a schematic representation of another embodiment of a filter system of the medical of FIG. 1 in a first position;

(14) FIG. 15 shows the filter system of FIG. 14 in a second position;

(15) FIG. 16 shows a schematic representation of a filter system of the medical device of FIG. 1 in a first position;

(16) FIG. 17 shows the filter system of FIG. 16 in a second position;

(17) FIG. 18 shows a schematic representation of another embodiment of a filter system of the medical device of FIG. 1;

(18) FIG. 19-21 show different transmissions along the circumferential and/or radial direction of the filter system in FIG. 18.

DETAILED DESCRIPTION OF THE INVENTION

(19) First, the structure of a medical device 1 is explained with reference to FIG. 1. The medical device can be an endoscope as shown or, alternatively, a microscope. The medical device 1 is configured to observe a partly fluorescent object 2 such as live tissue 3 which may comprise at least one fluorophore 4. The fluorophore 4 may have been added to the tissue 3 or be a natural constituent of the tissue 3. The fluorophore 4 absorbs light in an excitation waveband and emits light in an emission waveband, which preferably does not overlap the excitation waveband.

(20) The medical device 1 may comprise a light source 6 which emits a spectrum which comprises at least the excitation wavebands but is preferably wide band and also includes a greater part if not all of the visible light range. In particular, the light source 6 may emit white light in addition to any desired excitation wavebands, which may be in the infrared.

(21) The light 8 from the light source 6 is directed to the object 2 or the tissue 3, respectively. A diffractive element 10 such as a collimator or a lens may be arranged in an optical path 12 of the light 8. The optical path 12 extends around an optical axis 14.

(22) The medical device 1 further comprises a filter system 16 which is arranged in a filter plane 18. As shown, the filter plane 18 may extend perpendicular to the optical axis 14.

(23) In the filter plane 18, the filter system 16 comprises a filter area 20 and a transmission window 22. The filter area 20 comprises a band pass filter 24 having at least one passband, which comprises or, preferably, corresponds to the excitation wavebands of the at least one fluorophore 4.

(24) The transmission window 22 covers an area in the filter plane 18 which is smaller than the area occupied by the filter area 20. In the transmission window 22, the band pass filter 24 may be absent or present in an attenuated version, e.g. by being thinner.

(25) The band pass filter 24 may be a layer on a substrate 26. The substrate 26 is preferably a material which is transmissive at least in the excitation wavebands, preferably also in the visible light range. The substrate 26 has preferably low dispersion in the range of wavebands where it is transmissive and may be in particular a low dispersion optical glass.

(26) The medical device 1 may further comprise an optical system 28. The optical system may include fiber optics in the case of an endoscope, or a zoom magnifying lens in the case of a microscope. The optical system 28 may comprise further diffractive elements 30, such as at least one lense for directing light onto the object 2 or the tissue 3, respectively. A beam splitter system 32 or, in the case where the optical system 28 includes fiber optics, a bundle of wave guides collecting light from the object 2 or the tissue 3, may direct light towards a viewing system 34. The viewing system 34 allows a user of the medical device 1 to inspect an observation zone 36 from which light is directed to the viewing system 34. The viewing system 34 may be an ocular, such as a monocular or binocular, or a camera, such as an infrared camera, a NIR-camera, a thermographic camera, a multispectral camera, a hyperspectral camera or a colour camera, which all may also have three dimensional capabilities.

(27) Between the tissue 3 and the viewing system 34, another filter system 38 may be arranged, which may have the same or a different physical structure as the filter system 16. The filter system 38 in this instance may be an illumination filter system, restricting light to the excitation wavebands and to whatever additional wavebands the transmission window 22 allows to pass. The filter system 38 may be an observation filter system, which allows the emission wavebands and whatever the transmission window 22 allows to pass, to read the viewing system 34.

(28) Next, the function of the filter system 16 and 38 is explained with reference to FIGS. 2 to 4 which show various graphs where intensity I is shown over wavelength λ of electromagnetic radiation.

(29) In FIG. 2, the illumination spectrum 42 of the light source 6 is indicated. Further, the passband 44 of the band pass filter 24 is shown. The passband 44 corresponds to the excitation wavebands 46 of the at least one fluorophore 4.

(30) Illuminating the band pass filter 24 with light 8 having the spectrum 42 would result in a very narrow band illumination of the tissue 3. However, by providing the transmission window 22 in which either the band pass filter 24 is missing or attenuated, or in which a band pass filter having a wider passband is located, which wider passband 44 preferably includes the illumination spectrum 42, light 8 is allowed to pass also at frequencies, which are not included in the passband 44 of the filter area. Thus, the resulting spectrum 50 of the light 8 in the optical path 12 behind the filter system 16 is indicated by the broken line 15. The intensity in the side bands 52 adjacent to the passband 44 of the filter area depends on the transmission properties of the transmission window 22 and/or its relative size with respect to the filter area 20.

(31) FIG. 3 shows the combined effects of the filter systems 16 and 38 in medical devices for the observation of partly fluorescent tissue 3. The excitation spectrum 46 preferably does not overlap with the emission spectrum 54. The passband 44 of the filter area 20 comprises or, preferably, corresponds to the fluorescence emission spectrum 54 of the at least one fluorophore 4.

(32) The filter system 38 in a conventional medical device 1 filters out all frequencies except those resulting from fluorescence, as shown in FIG. 3. This leaves areas of the tissue 3 where there is no fluorophore 4 and thus no fluorescence dark and hard to inspect.

(33) By providing a transmission window 22, as shown in FIG. 4, both in the illumination filter system 16 and in the observation filter system 38, the side bands 52 are allowed to pass both the illumination filter system 16 and observation filter system 38, resulting in an observation spectrum 58 which includes the side bands 52 from the illumination spectrum 42 and the excitation wavebands 46.

(34) FIG. 5 shows a view onto a filter system 16, 38 along the optical axis 14. The filter system 16, 38 may be supported in a frame structure 60, which may form an aperture 62 in the filter plane 18. The transmission window 22 is seen to have at least a one-axial symmetry about the optical axis 14 by forming a rectangular area.

(35) In FIG. 6, the cross sectional view along the line VI-VI in FIG. 5 is shown. The transmission window 22 in this instance is characterized by an absence of at least one filter material or filter layer 64 which forms the band pass filter 24. Thus, the incident light can pass through the substrate 26 without any spectral attenuation. Alternatively to the situation shown in FIG. 6, the band pass filter 24 may still be present in the transmission window 22 but have smaller attenuation in the passband 44 than in the filter area 20. Alternatively or cumulatively, the transmission window 22 may be provided with a grey filter.

(36) The effect of providing both the transmission window 22 and the filter area 20 in the filter system 16, 38 is shown in FIG. 7. The incident light 8 passes the transmission window 22 basically unfiltered, at least with significantly less attenuation than the incident light in the filter area. This is shown by the thicker arrow 65 of light being transmitted through the transmission window 22 and the comparatively thinner arrow 66 representing light from the light source 6 which has passed through the filter area 20 and is thus restricted to the passband 44.

(37) The direct transmission of light 8 through the transmission window 22 as shown in FIG. 7 may lead to glare in some instances. To avoid this, a transmission window 22 and a filter area 20 may not only be provided on a first side 68 of the substrate 26, as in FIGS. 6 and 7, but also on a second side 70 of the substrate 26 where the first side 68 faces the incident light 8 and the second side 70 faces away from the incident light 8.

(38) The filter area 20, which in this case may not necessarily be the same band pass filter 24 as on this first side, but may also be a light-blocking filter such as a grey filter or an opaque mask, is preferably congruent to the transmission window 22 and/or covers the transmission window completely. Thus, light which is incident perpendicular to the filter plane 18 cannot pass directly though the filter system 16, 38.

(39) As already stated above, the actual shape and size of the transmission window 22 and the filter area 20 can vary. However, in order to obtain a homogeneous distribution of light in the observation zone 36 or the viewing system 34, a symmetric shape with respect to at least one radial direction 71 through the optical axis 14 is preferred.

(40) The light flux through the transmission window 22 should be small in order to maintain high contrast in the fluorescence emission wavebands. In particular, the total light flux 65 through the transmission window 22 is at most 1% of the total light flux 66 through the transmission window. In another embodiment, the surface area of the transmission window 22 may comprise no more than 1% of the area of the aperture 62. To be able to observe non-fluorescent regions, the total light flux 65 may not be less than 0.1‰ of the total light flux through the filter area 20. It may be further preferred that the area of the transmission window 22 is at least 0.1‰ of the area of the aperture 62.

(41) In FIG. 9, the transmission window has four axes of symmetry 72 by being cross-shaped. Of course, other shapes such as circular may also be used.

(42) The transmission window 22 may be comprised of separate transmission regions 74, which are preferably arranged in an array which also exhibits an at least one-axial symmetry about the optical axis 14.

(43) For example, as shown in FIG. 10, a symmetric array of quadratic transmission regions 74 may together form the transmission window 22. The discrete transmission regions 74 are separated from one another by the filter area 20. Of course, the arrangement shown in FIG. 10 can also be reversed. Thus, the filter area 20 may be divided in separate filter regions between which the transmission window 22 is arranged. In such an embodiment, the relatively smaller area of the transmission window 22 compared to the filter area 20 should be still maintained.

(44) In FIG. 11, it is shown that the transmission window 22 and the filter area 20 may be concentric rings arranged alternatingly.

(45) In the following embodiments, the filter system 16, 38 may comprise at least one moveable filter element 80 which is operatively connected to an actuator 82 such as an electric, mechanic or magnetic drive, or a manually operated drive.

(46) The moveable element 80 is configured to be moved from a first position 84 to a second position 86 relative to the optical axis. In the first position 84, the area in the optical path of the transmission window 20 is smaller than in the first position. In the first position, there may even be no transmission window 22 present as exposed to the incident light. Thus, by moving the moveable filter element from the first position 84 to the second position 86, the intensity of the light 8 in the side bands 52 can be increased.

(47) A first embodiment having a moveable filter element 80 is shown in FIGS. 12 and 13. In this embodiment, the moveable filter element 80 is a moveable cover mask 88, which may be translatably and/or as shown in FIGS. 12 and 13 rotatably supported in the medical device 1. In the first position 84, a larger area or all of the transmission window 22 is covered by the cover mask 88. In the second position 86, shown in FIG. 13, a smaller part of the transmission window 22 is covered by the cover mask 88. The cover mask 88 may be an opaque filter or be at least partly transmissive and have band pass characteristics. For example, the cover mask 88 may have the same band pass properties as the filter area 20.

(48) The cover mask 88 may be held in a region 90 beyond the aperture of the filter system 16, 38 for actuation by the actuator 82.

(49) The moveable filter element 80 may, in another embodiment, comprise a filter area 20 which is moved at least partly out of the optical path 12 to create or enlarge the transmission window 22. This is shown in two embodiments in FIGS. 14 and 15, and in FIGS. 16 and 17.

(50) According to the embodiment of FIGS. 14 and 15, the filter system 16, 38 may be split into at least one stationary part 92 comprising part of the filter area 20 and at least one moveable element 80 comprising the remainder of the filter area 20. By moving the moveable filter element 80 away from the stationary filter element 92, a transmission window 22 is created or enlarged in the second position 86 as shown in FIG. 15. At least one of the moveable filter element 80 and the stationary filter element 92 may already be provided with a transmission window to always allow a minimum of light 8 in the side bands 52 to pass the filter system 16, 38. In an alternative embodiment, at least two movable filter elements may be provided which are configured to be moved away from each other to open the transmission window 22.

(51) To allow a gapless closing between the at least one moveable filter element 80 and the at least one stationary filter element 92, a stationary cover 94 may be provided which covers the junction between the two filter elements 80, 92.

(52) In the embodiment of FIGS. 16 and 17, a unitary moveable filter element 80 extends across the whole aperture 62 of the filter system 16, 38. The moveable filter element 80 may, in the first position 84 form a continuous filter area 20 across the filter plane 18. In the second position 86, the moveable element 80 is moved, preferably within the filter plane 18 at least partly out of the aperture so that a transmission window 22 opens.

(53) In the embodiment of FIG. 18, the transmission of the filter area 20 and/or the transmission window 22 varies in a circumferential direction 95 around the optical axis 14. The transmission may, in particular, vary between 0% and 100%. For example, the transmission in the passband of the filter area 20 may be constant across the aperture 62 whereas the transmission in the passband of the transmission window 22 may vary. The variation may be continuous in the circumferential direction 95 or stepwise, e.g. having sectors of preferably equal size, each sector having a different transmission than the neighboring sectors.

(54) Instead of or in addition to the circumferential variation of transmission, the transmission may also vary in a radial direction 96 from the optical axis 14 outwards.

(55) In the embodiment of FIG. 18, the filter area 20 and the transmission window 22 are not discrete areas but overlap each other in that the respective layers which cause the filtering properties of the filter area 20 and the transmission window 22 are put onto one another.

(56) In FIG. 19-21, the transmission of the filter area 20 or the transmission window 22 is shown unfolded in the circumferential 95 and/or radial 96 direction. Dark areas are areas in which there is no light passing through the respective passband.

(57) In FIG. 19, it is shown that in the circumferential 95 and/or radial 96 direction, the transmission of the filter area 20 may be constant. For example, the filter area may be transmissive in the emission and/or excitation waveband or wavebands of a fluorophore.

(58) According to FIG. 20, the varying transmission is created by a dithering with varying densities. The dithering is done by applying a cover mask 88 to small regions of the aperture. This can be done by applying as a cover mask 88, a layer which is opaque in the passband of the transmission window 22 but translucent in the passband of the band pass filter. Between the individual cover masks 88, transmission regions 74 are situated through which light in the passband may pass. The density of the individual cover masks 88, i.e. the percentage of area covered by the transmission regions 74 between the individual cover masks 88, determines the amount of light in the passband passed through the transmission window 22.

(59) In FIG. 21, a filter system 16, 38 is shown in which the transmission varies continuously in the circumferential 95 and/or radial 96 direction. This can, e.g. be done by varying the thickness of a filter layer which blocks light in the passband of the transmission window.

(60) The type of variation in the circumferential 95 and/or radial 96 direction may depend on the application and be linear and/or non linear.

(61) The filter system 16, 38 which has been explained with reference to FIGS. 18-21, may be varied in that the transmission in the transmission window 22 is constant across the aperture 62, whereas the transmission of the band pass filter varies in the circumferential 95 and/or radial 96 direction. Also, a variation of both the transmission of the band pass filter and the transmission window in the circumferential 95 and/or radial 96 direction is possible.

REFERENCE NUMERALS

(62) 1. Medical device 2. Object 3. Tissue 4. Fluorophore 6. Light source 8. Light from light source 10. Diffractive element 12. Optical path 14. Optical axis 16. Illumination filter system 18. Filter plane 20. Filter area 22. Transmission window 24. Band pass filter 26. Substrate 28. Optical system 30. Diffractive element 32. Beam splitter system 34. Viewing System 36. Observation zone 38. Observation filter system 42. Illumination spectrum of light source 44. Passband of filter area of illumination filter system 46. Fluorescence excitation waveband 48. Passband of transmission window 50. Resulting spectrum 52. Side band 54. Fluorescence emission spectrum 56. Passband of filter area of observation filter system 58. Observation spectrum 60. Frame structure 62. Aperture 64. Filter layer 65 Thick arrow 66. Thin arrow 68. First side of substrate 70. Second side of substrate 71 Radial direction 72. Axis of symmetry 74. Transmission region 80. Moveable filter element 82. Actuator 84. First position 86. Second position 88. Cover mask 90. Region outside aperture 92. Stationary part of filter system 94. Stationary cover 95 Circumferential direction 96 Radial direction I Intensity λ Wavelength