IN SITU TWO-PHASE EXTRACTION SYSTEM

Abstract

The present invention is related to a fermentative production of retinoids, including retinol or retinyl acetate, comprising cultivation of a retinoid-producing host cell, such as fungal host cell, particularly oleaginous host such as e.g. Yarrowia, in a two-phase culture system in the presence of vegetable oil as second phase. The retinoids present in the vegetable oil can be used without further isolation or purification steps as active ingredients in the food, feed, pharma or cosmetic industry.

Claims

1. Process for in situ extraction of retinoids in a fermentation process, comprising cultivation of the host cell, particularly fungal host cell, preferably oleaginous yeast, more preferably Yarrowia, in the presence of a second phase lipophilic solvent comprising vegetable oil.

2. Process according to claim 1, wherein the host cell is grown on a carbon source selected from glucose or ethanol.

3. Process according to claim 1, wherein the percentage of retinoids is increased by at least about 10% compared to a process using silicone oil as second phase lipophilic solvent.

4. Process according to claim 1, wherein the solvent is used for collection and extraction of the fermentative produced retinoids.

5. Process according to claim 1, wherein the fermentative produced retinoids comprise a percentage of at least about 0.001 to about 30% retinyl acetate based on total retinoids.

6. Process according to claim 1, wherein the vegetable oil is selected from the group consisting of oleic, palmitic, steric or linoleic acid and glycerol, preferably selected from the group consisting of corn oil, olive oil, cottonseed oil, rapeseed oil, sesame oil, canola oil, safflower oil, sunflower oil, soybean oil, grapeseed oil, and peanut oil, more preferably selected from corn oil.

7. Process according to claim 1, wherein the host cell is selected from oleaginous host cell including Rhodosporidium, Lipomyces or Yarrowia, preferably Yarrowia lipolytica.

8. Process according to claim 1, wherein the host cell comprises one or more genetic modifications, particularly reduction or abolishment of endogenous lipase activity, preferably reduction or abolishment of lipase activity corresponding to Yarrowia lipase activity 2, 3, 4, and/or 8, more preferably Yarrowia lipase activity 8.

9. Process according to claim 1, wherein the host cell is transformed with and is expressing heterologous genes, preferably genes encoding acetylating enzymes catalyzing the conversion of retinol to retinyl acetate, more preferably fungal enzymes, most preferably originated from Lachancea mirantina.

10. Process according to claim 9, wherein the acetylating enzyme comprises one or more amino acid substitution(s) in a sequence with at least about 20%, such as e.g. 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 92, 95, 97, 98, 99% or up to 100% identity to SEQ ID NO:12, wherein the one or more amino acid substitution(s) are located at position(s) corresponding to amino acid residue(s) selected from the group consisting of position 68, 69, 72, 73, 171, 174, 176, 178, 291, 292, 294, 301, 307, 308, 311, 312, 320, 322, 334, 362, 405, 407, 409, 480, 483, 484, 490, 492, 520, 521, 522, 524, 525, 526 and combinations thereof in a polypeptide according to SEQ ID NO:12.

Description

EXAMPLES

Example 1: General Methods and Strains

[0056] All basic molecular biology and DNA manipulation procedures described herein are generally performed according to Sambrook et al. (eds.), Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press: New York (1989) or Ausubel et al. (eds). Current Protocols in Molecular Biology. Wiley: New York (1998). All genetic manipulations exemplified were performed in Yarrowia lipolytica.

[0057] Shake plate assay. Typically, 200 l of 0.075% Yeast extract, 0.25% peptone (0.25YP) is inoculated with 10 l of freshly grown Yarrowia and overlaid with 200 l of Drakeol 5 (Penreco, Karns City, PA, USA) mineral oil, silicone oil, or corn oil with either 2% oleic acid or 2% glucose as a carbon source. Clonal isolates of transformants were grown in 24 well plates (Multitron, 30 C., 800 RPM) in YPD media with one of the overlays indicated earlier for 4 days. The overlay fraction was removed from the shake plate wells and analyzed by HPLC on a normal phase column, with a photo-diode array detector.

[0058] DNA transformation. Strains are transformed by overnight growth on YPD plate media 50 l of cells is scraped from a plate and transformed by incubation in 500 l with 1 g transforming DNA, typically linear DNA for integrative transformation, 40% PEG 3550MW, 100 mM lithium acetate, 50 mM Dithiothreitol, 5 mM Tris-Cl pH 8.0, 0.5 mM EDTA for 60 minutes at 40 C. and plated directly to selective media or in the case of dominant antibiotic marker selection the cells are out grown on YPD liquid media for 4 hours at 30 C. before plating on the selective media. Nourseothricin (Nat) selection was performed on YPD media containing 100 g/mL nourseothricin and hygromycin (Hyg) selection was performed on YPD containing 100 g/mL hygromycin. URA3 marker recycling was performed using 5-fluoroorotic acid (FOA). Episomal hygromycin resistance marker (Hyg) plasmids were cured by passage on non-selective media, with identification of Hyg-sensitive colonies by replica plating colonies from non-selective media to hygromycin containing media (100 g/mL).

[0059] DNA molecular biology. Plasmid MB9523 containing expression systems for DrBCO, LmATF-S480Q_G409A_V407I_H69A_1484L, and FfRDH (SEQ ID NO:10) was synthesized at Genscript (Piscataway, NJ, USA). Plasmid MB9523 contains the URA3 for marker selection in Yarrowia lipolytica transformations. For clean gene insertion by random nonhomologous end joining of the gene and marker a Sfil plasmid fragment of interest from MB9523 was purified by gel electrophoresis and Qiagen gel purification column. Clones were verified by sequencing. Typically, genes are synthesized by a synthetic biology at GenScript (Piscataway, NJ). Plasmid MB8388-LIP8 (SEQ ID NO:11), containing a Cas9, and guide RNA expression systems to target LIP8, was synthesized at Genscript (Piscataway, NJ, USA).

[0060] Plasmid list. Plasmid, strains, nucleotide and amino acid sequences that were used are listed in Table 1, 2 and the sequence listing. In general, all non-modified sequences referred to herein are the same as the accession sequence in the database for reference strain CLIB122 (Dujon B, et al, Nature. 2004 Jul. 1; 430(6995):35-44).

TABLE-US-00001 TABLE 1 list of plasmids used for construction of the strains for overexpression or deletion of the respective genes indicated as insert. LmATF1-mut refers to Lachancea mirantina (LmATF1; SEQ ID NO:13 in WO2019058001) carrying aa substitutions S480Q_G409A_V407I_H69A_I484L. DrBCO refers to BCO originated from Danio rerio (see SEQ ID NO:18 in WO2020141168); FfRDH refers to RDH originated from Fusarium (see SEQ ID NO: 22 in WO2020141168). For more explanation, see text. SEQ Plasmid Insert Marker ID NO: MB7452 Cas9 Nat 9 MB9523 DrBCO; LmATF1-mut; FfRDH URA3 10 MB8388-LIP8 Cas9; LIP8 targeting guide RNAs Hyg 11

TABLE-US-00002 TABLE 2 list of Yarrowia lipolytica strains used. Construction of ML17544 is described in Table 2 of WO2020141168. For more details, see text. Strain Description ML18812 ML17544 transformed with MB9523 ML18812-lip8 ML18812 with MB7542 and MB8388-LIP8, lip8 deletion

[0061] Retinoid quantification. Analysis of retinoids were carried out with a C4 reverse phase retinoid method (see below) and C18 as described elsewhere (WO2020141168). The addition of all added intermediates gives the total amount of retinoids.

[0062] C4 reverse phase chromatography. For exact determination of discrete retinoids the long run reverse phase system was used. We separated analytes at 230 nm and 325 nm through the Agilent 1290 instrument with YMC Pro C4, 1503.0 mm 3 m column (YMC America, Allentown PA) stationary phase, and a 5 l injection loop volume and column and sample tray controlled at 23 C. with gradients described in Table 4B. Analytes were detected at 230 nm and 325 nm and the peaks identity verified with LCMS. The analytes separated as discrete peaks that were assigned according to Table 4A.

TABLE-US-00003 TABLE 4A list of analytes using C4-reverse phase method. The addition of all added intermediates gives the total amount retinoids. RT means retention time. For more details, see text. Intermediates RT [min] max [nm] trans-retinol 20.21 325 cis-retinol 20.32 325 dihydro-retinol 20.75 290 trans-retinal 20.89 380 cis-retinal 21.02 380 trans-retinyl-acetate 22.15 325 cis-retinyl-acetate 22.35 325 dihydro-retinyl acetate 22.60 290 retinyl esters 26-30 325

TABLE-US-00004 TABLE 4B UPLC Method Gradient with solvent A: acetonitrile; solvent B: water; solvent C: water/acetonitrile/ methanesulfonic acid 1000:25:1. For more details, see text. Time % % % Flow [min] A B C [ml/min] 0 5 85 10 0.5 20 98 0 2 0.5 35 98 0 2 0.5 35.1 5 85 10 0.5 40 5 85 10 0.5

[0063] Method Calibration. Method is calibrated using high purity retinyl acetate received from DSM Nutritional Products, Kaiseraugst, CH. Retinols and retinal are quantitated against retinyl acetate. Dilutions described in Table 4C are prepared as follows. 40 mg of retinyl acetate is weighed into a 100 mL volumetric flask, and dissolved in ethanol, yielding a 400 g/mL solution. This solution is sonicated as required to ensure dissolution. 5 mL of this 400 g/mL solution is diluted into 50 mL (1/10 dilution, final concentration 40 g/mL), 5 mL into 100 mL (1/20 dilution, final concentration 20 g/mL), 5 mL of 40 g/mL into 50 mL (1/10 dilution, final concentration 4 g/mL), 5 mL of 20 g/mL into 50 mL (1/10 dilution, 2 g/mL), using 50/50 methanol/methyl tert-butyl ether (MTBE) as the diluent. All dilutions are done in volumetric flasks. Purity of retinyl acetate is determined by further diluting the 400 g/mL stock solution 100-fold (using a 2 mL volumetric pipet and a 200 mL volumetric flask) in ethanol. Absorbance of this solution at 325 nm using ethanol is taken as the blank, with adjustment of the initial concentration using the equation (Abs*dilution (100)*molecular weight (328.5)/51180=concentration in mg/mL). Because of quick out-maximization of UV absorbance of retinyl acetate, lower concentrations are better.

TABLE-US-00005 TABLE 4C preparation of calibration standards. For more explanation, see text. Stock [RA], Final dilution concentration 20 g/mL, 1/10 2 g/ml 40 g/mL, 1/10 4 g/mL 400 g/mL, 1/20 20 g/mL 400 g/ml, 1/10 40 g/mL

[0064] Sample preparation. Top second phase layer samples from each strain were diluted at a 25-fold dilution or higher into tetrahydrofuran (THF). Fermentation whole broth was prepared using a 2 mL Precellys (Bertin Corp, Rockville, MD) tube, add 25 l of well mixed broth and 975 l of THF. Precellys 3157500 rpm for two cycles with a freeze at 80 C. for 10 minutes between cycles. Cell debris was spun down via centrifugation for 1 minute at 13000 rpm. These samples were diluted 10-fold in THF.

[0065] Fermentation conditions. Fermentations used a silicone oil or corn oil overlay and stirred tank that was glucose fed in a bench top reactor with 0.5 L to 5 L total volume (see WO2016172282, Ex. 5 and 6). Generally, the same results were observed with a fed batch stirred tank reactor with an increased productivity demonstrating the utility of the system for the production of retinoids. Preferably, fermentations were batched with 6% glucose and 20% silicone oil or 20% corn oil was added after dissolved oxygen dropped below about 20% and feed was resumed to achieve 20% dissolved oxygen throughout the feeding program. Fermenters were harvested and compared at 138 h.

Example 2: Deletion of LIP8 in Yarrowia lipolytica to Enable Use of Corn Oil as a Second Phase

[0066] Lipase gene LIP8 was disabled in strain ML18812 using modern CRISPR Cas9 methods, to generate strain ML18812-LIP8. Briefly, strain ML18812 was transformed with MB7452 (SEQ ID NO:9), which contains an expression module for Cas9 and the nourseothricin selection marker. Nourseothricin resistant transformants (selected on YPD with 200 g/mL nourseothricin) were subsequently transformed with plasmid MB8388-LIP8 (SEQ ID NO:11), which contains expression sequences for Cas9, and guide RNAs with seed sequences targeting LIP8, and the hygromycin resistance marker. Transformants (selected on YPD with 200 g/mL hygromycin) were screened for mutation by Sanger sequencing with primers flanking the targeted region. Strain ML18812-LIP8 was found to have inactivating mutations in LIP8.

[0067] Strains ML18812, which is wild-type strain, i.e. wherein the endogenous lipase genes are still active, and strain ML18812-LIP8, which carries the LIP8 deletion, were growing in a fermentor as described in Example 1, with glucose as carbon source and either corn oil or silicone as second phase. Second phase was measured for the presence of retinoids, the results are shown in Table 5.

TABLE-US-00006 TABLE 5 retinoid production in strain ML18812-LIP8 (deletion of LIP8) compared to wild-type strain ML18812 (LIP+) as control together with silicone oil or corn oil as second phase. The number for ML18812 with silicone oil is set to 100%, the other numbers are calculated in relation thereto. For more explanation, see text. Relevant Total retinoids lipase (% of silicone oil) Strain genotype Silicone oil Corn oil ML18812 LIP+ 100 6.3 ML18812-LIP8 deletion lip8 95 110

[0068] While both strains produced comparable levels of retinoids in silicone oil, strains with LIP8 deletion/inactivation had significantly higher retinoids in corn oil second phase than strains with wild-type LIP8.

[0069] In addition, the volume of second phases from each of the four conditions was measured. Results are shown in Table 6 below.

TABLE-US-00007 TABLE 6 impact of LIP8 deletion on different second-phase solvents. For more explanation, see Table 5 or text. % second phase remaining Relevant at end of fermentation lipase compared to beginning Strain genotype Silicone oil Corn oil ML18812 LIP+ >95% <5% ML18812-LIP8 deletion lip8 >95% >95%

[0070] While the second phase amounts were unchanged between WT and LIP8-deletion strains for silicone oil, strains with wild-type LIP8 had a marked reduction in the amount of corn oil second phase remaining in the fermentor. Strains in which LIP8 was inactivated did not have any significant reduction in the amount of corn oil second phase over the course of the fermentation.

TABLE-US-00008 TABLE7 sequences.SEQIDNOs:2,4,6,8referto thepolynucleotidesexpressingLIP2,LIP3, LIP4,LIP8,respectively,accordingto SEQIDNOs:1,3,5,7.formore explanation,seetext. SEQ ID NO: sequence5to3 1 MKLSTILFTACATLAAALPSPITPSEAAVLQKRVYTSTETSHIDQESYNE FEKYARLANIGYCVGPGTKIFKPFNCGLQCAHFPNVELIEEFHDPRLIED VSGYLAVDHASKQIYLVIRGTHSLEDVITDIRIMQAPLTNVTGHSLGGAA ATCDDCLVHNGFIQSYNNTYNQIGPKLDSVIEQYPDYQIAFDLAANISST ALLFGINLKVNGHDPLVVTLGQPIVGNAGFANWVDKLFFGQENPDVSKVS KDRKLYRITHRGDIVPQVPFWDGYQHCSGEVFIDWPLIHPPLSNVVMCQG QSNKQCSAGNTLLQQVNVIGNHLQYFVTEGVCGI 2 atgaagctttccaccatcctcttcacagcctgcgctaccctggctgccgc cctcccttcccccatcactccttctgaggccgcagttctccagaagcgag tgtacacctctaccgagacctctcacattgaccaggagtcctacaacttc tttgagaagtacgcccgactcgcaaacattggatattgtgttggtcccgg cactaagatcttcaagcccttcaactgtggcctgcaatgtgcccacttcc ccaacgttgagctcatcgaggagttccacgacccccgtctcatctttgat gtttctggttacctcgctgttgatcatgcctccaagcagatctaccttgt tattcgaggaacccactctctggaggacgtcataaccgacatccgaatca tgcaggctcctctgacgaactttgatcttgctgctaacatctcttctact gctacttgtgatgactgtcttgtccacaatggcttcatccagtcctacaa caacacctacaatcagatcggccccaagctcgactctgtgattgagcagt atcccgactaccagattgctgtcaccggtcactctctcggaggagctgca gcccttctgttcggaatcaacctcaaggttaacggccacgatcccctcgt tgttactcttggtcagcccattgtcggtaacgctggctttgctaactggg tcgataaactcttctttggccaggagaaccccgatgtctccaaggtgtcc aaagaccgaaagctctaccgaatcacccaccgaggagatatcgtccctca agtgcccttctgggacggttaccagcactgctctggtgaggtctttattg actggcccctgatccaccctcctctctccaacgttgtcatgtgccagggc cagagcaataaacagtgctctgccggtaacactctgctccagcaggtcaa tgtgattggaaaccatctgcagtacttcgtcaccgagggtgtctgtggta tctaa 3 MPLELPSLNASIVGNTVQNGAVEQFLNIRYADIPGKFEKPVLKNDWNGAE IDATKVGPVCPQPRTPFNFFSVPDDLWEKVNVDTYQDGLLCDNLIVTRPK GVSANARLPTVVWIHGGSNIEGSIYNLIYEPQFLVAESVRVGKPIVHVCI EYRLGLAGFLTKNGKGNWGTWDQYTGCQWVNRHIQDFGGDPLNVTLTGES AGSVAVHNMLIKDSMNGRKLFRNAVMMSGTLETITPQPPKWHARLEEKVA KVTGKEVADLASLSDKELLDAQIKLNVAVCMTCDDGDFFEPGWKQHLTPD WLDKLIISDCKDEGMLYFLPVNAQDDEELLAKVAKSPVGKEISELYGIKE GGDIKSACLDLKTDATFNYFNHLLEKKMEEARNNGSTSRVYRLAVDEPNP HNPDQRAHHAVDVLYMENSTKFNEHGDKLSRLFQSHELRLAYGLEPWDHR NFGVYRNGGYQQLPLSELNKVRPVERYEALSKMDFGQVGRLSNALSRL 4 atgcctctcgaactcccctcgctcaacgcctcgattgtcggcaacaccgt tcagaacggcgctgttgagcagtttctcaacatccgatacgccgacattc ctggcaagtttgagaagcccgtgctcaagaacgattggaacggcgcggag atcgacgccaccaaggtcggtcccgtgtgcccccaaccccgcaccccatt caacttcttctccgtgccagacgacctctgggagaaagtcaatgtggaca cgtaccaggacggtctgctgtgcgacaacctgattgtgacgcgaccgaag ggcgtgtctgccaacgcccggctgcccactgttgtgtggatccacggcgg ctccaatattgagggcagtatctacaacctcatctatgagccccagttcc tggtggcagagtcggtgcgagtaggcaagccgattgtgcacgtgtgtatc gagtaccgactgggtctcgcgggcttcctcaccaagaacggcaagggcaa ctggggcacgtgggatcagtacacgggctgccagtgggtcaaccgccaca ttcaggactttggaggcgatcctttgaacgtgacattgaccggtgagtct gccggctctgtagcagtccataacatgctcatcaaggactccatgaacgg ccgaaagttgttccgaaatgccgtcatgatgagtggcactctcgagacca tcactcctcagcctcccaagtggcatgctcgtttggaggagaaggtggcc aaggtcactggcaaggaagtggccgaccttgcttctctgtccgataagga gctgctcgacgcccagatcaagctcaatgtggctgtgtgcatgacttgcg acgacggcgactttttcgagcccggatggaagcagcatctgactcctgac tggctcgacaagctcatcatctccgattgcaaggacgagggcatgctgta tttcctgccagtcaacgcgcaggacgacgaggagctgttggcaaaggtgg ccaagtcgcccgtgggtaaggagatttccgagctttacggcatcaaggag ggtggcgatatcaagtctgcgtgtctcgatctcaagactgacgccacctt caattactttaaccatctgctgttcaagaagatggaggaggcccgaaaca acggctccacttctcgagtttaccgtctggccgtcgatgagcccaacccc cacaaccccgaccagcgggcccaccacgccgtcgacgtgctgtacatgtt caactcgaccaagttcaacgagcacggcgacaagctgtctcggctgttcc agagccactttttgcggctggcgtatggcctggagccctgggaccatcga aactttggagtgtacagaaacggcggctaccagcagctgccgctgagtga gttgaacaaggtccgacccgtcgagcggtacgaggcgctgtccaagatgg actttggccaggttgggcgtttgtccaatgcgctttcgcgcctatga 5 MVSLSARIKDFFSVLLLGAATITPSTQTAGVSQGFYDFARDFAHLSNIAY CVNAPITPLNPDFTCGNSCKHFPEIELVKTFGGNFFKTSITGYLAVDHVK KEKYVVERGTFSLADAITDMQFQLSPELVDVPALNTFSANDTTAEAQTHC EGCKIHDGESKAFTETWGNIGEDLQKHLDANPDYQLYVTGHSLGAAMALL GATSIKLKGYDPILINYGQPRVGNKPFAEFINKLWFGEGNGLEITPERKL YRMTHWNDIFVGLPNWEGYTHSNGEVYINNRFINPPLKDVISCAGGENSK CYRSSESLLSQINLLQNHLAYIDYIGYCALNIGRRELADQEHYTGPYYYG HRSEEDFKKLGLELSTPQVEN 6 atggtatccctctctgctcgaatcaaagactttttttcggtcctcctcct cggagctgcaaccatcactccctccacacagaccgcaggcgtgtctcaag ggttctatgattttgctcgggactttgcccatctgtccaacattgcctac tgtgtcaatgctcccatcactccactgaacccggacttcacctgtggcaa ctcgtgcaagcactttccggaaattgagcttgtgaagacatttggaggca acttcttcaagacctccattacgggctacctggctgtcgatcatgtcaag aaggagaagtacgttgtcttccgaggaaccttttcgctggcagacgcgat cacggacatgcagttccagctgtctcctttcctggtcgatgtgcctgccc tgaacactttctcagctaatgacaccaccgcagaggcccagacgcactgt gagggctgcaaaattcacgacggcttctccaaggcctttaccgagacctg gggtaacattggtgaggatctgcagaaacacctggacgctaacccggact accagctgtacgtgactggccattctctgggagctgctatggcccttctt ggagctacttccatcaagctcaagggctacgatcccattctcatcaacta cggacagccccgagtcggaaacaagcccttcgctgagttcattaacaagt tgtggtttggagaaggcaacggtctggaaatcacccccgagagaaagctg taccgaatgacccactggaacgacatctttgttggcctgcccaactggga gggatacacccactctaacggtgaagtatacatcaacaaccggttcatca accctcctctcaaggatgtcatctcttgtgctggaggcgaaaactcgaag tgctaccgatcctcgttcagcctgctgtcccagatcaatctgctccaaaa ccacctggcttacattgattacattggatactgcgctctgaacattggtc gacgagagcttgccgatcaggaacattacactggtccttattactatggt catcgatctgaggaggactttaagaagttgggcttggagctatccacccc acaagttgagaactga 7 MAGFNFTFGQVISYLASMLYGQVDATSSSTRIQATQDLYDFTAKFSRLSN IAYCINAPFTPLRTDFTCGESCRYFPDLQLDSVFGGNFSSASTTGYIAYD HKKKEKYIVFRGTFSIPDIITDIQFQTAPWLTSLPTHLIPTKEDFEHKQA ILKHYAAENKGLSNLEERQDVVHEDPSLVPKKMDKCENCQIHDGFAKGFN ETIEHAGPQIEKFLGNNTDYKMFVVGHSLGAAQAQLFATQFKLLGFDPYM INFGQPRIGNPEFAAYINQLWFNDTGLVVNDARRFYRVTHWNDIVVGVPD WLNYTHSIGEVFIDEESVYPKLDKVVVCEGGENPLCHRGTFNLWSRINEL QNHLAYIFYIGLCAFNIGRRDVLNMPQYQGNFSYQHNIDPNYNYDTKVPT RISKSN 8 atggcagggttcaacttcactttcgggcaggtgatttcgtacctggcttc catgctctatggacaggtggatgccacttcatcctccactcgaatccagg ccacccaggatctgtacgacttcacagccaagttttcgcgactctcaaac atcgcgtactgcatcaatgcccccttcacgcctctcagaacggacttcac ctgcggagaaagctgtcggtacttccccgacctccagctggactcagtgt ttggtggtaacttctcctcagcctccactaccggctacattgcatacgac cacaagaagaaggaaaagtacattgtgtttcgaggaactttcagtatccc tgatatcatcacagacattcaatttcagactgccccttggttgacctctc tgcccacgcatctgatccctaccaaggaggactttgaacacaagcaggcc atcctgaagcactacgctgccgaaaacaagggtctcagcaacctggaaga gcgacaggatgttgtgcatgaagaccctagcctggttcccaagaaaatgg acaagtgcgagaactgccagatccatgacggattcgccaagggcttcaac gagactatcgagcatgccggaccccagattgaaaagttcctgggcaataa caccgactacaagatgtttgttgttggccactctctaggagctgctcagg cccagctgtttgctacacagttcaaactgctgggatttgacccttacatg atcaactttggacagcctcgacttggaaaccctgagttcgccgcctacat caaccagctgtggttcaacgatactggtctggttgtcaatgatgcccgac gattctaccgagtgactcactggaacgatatcgtcgtgggagtgcccgac tggctcaattacacccactctatcggagaggtgttcatagacgaggagag cgtttaccccaagctggacaaggtggtggtgtgcgagggaggagagaacc ccctgtgccaccgaggaactttcaacctgtggtcacgaatcaacttcctg cagaaccatttggcttatatcttctacattggtctgtgtgctttcaacat tggccgaagagacgtgctcaacatgccacaataccagggcaacttctcgt accagcacaacatcgaccccaactacaattacgataccaaggttcccacc cggatcagtaaatcaaactaa 9 cgcgtggatcgccggtgcgttgacgttggtgacctccagccagaggtgcc cggcgccccgctcgccggcgaactccgtcgcgagccccatcaacgcgcgc ccgaccccgtgcccccggtgctccggggcgacctcgatgtcctcgacggt cagccggcggttccacgccgagtacgagatgaccacgaagcccgccaggt cgccgtcgtccccgtacgcgacgaacgtccgggagtccgggtcgccgtcc tccccgtcgtccgattcgtcgtccgattcgtcgtcggggaacaccttggt caggggcgggtccaccggcacctcccgcagggtgaagccgtccccggtgg cggtgacgcggaagacggtgtcggtggtgaaggacccatccagtgcctcg atggcctcggcgtcccccgggacactggtgcggtaccggtaagccgtgtc gtcaagagtggtcatttttgtgtctaggtgtttgtgtttggactgcgatc agtgaagaaaagaagaggaaaaattgtgcaagaaattttgctttcaagac ttggctgatgcagcagggtaactctgggacacagacctatgtttgtggtt aaactcaatgcacgtggtacgtgcgtggagcgcttacccatccaagggtg tggacatggaaccgacggtccgtggagttgtgtaatgtcattttggcgac tcttgaagcaaggctataaaaaaattgtgtggcttgagtcttatcgagct cggtcactacaagagttaatcttcctgtctcaggcagacaggtcaggcag ggttacttttgggtgtgctgtaactcactgtatggccgttagtgcgcata gacgttgtacatactggaccgaattgtagcgtgctcaatagggccaataa agctattgtagggatccgaattttcagaacctaatttatctgttacccgg cctgtggctcgcacagcttaaaaatggtcaaactttccccttcttgtctt tttttcctcacattcatcaggttcttgtcttgatctttcaagtgagtatt aattaccgaccttggttcttcattgggagagcattggaagccgtggtgca gcaaccacaaaacggttcttccccttcgataccttcttgcctgcctttca atacaagtcggctcgattagcggtggtcgcccccgccagcggagaacatg gaactaacccagaatgagagctaagtggagaaagaagagagtcagacgac tcaagcgaaagcgccgcaaggtccgagctcgatccaaataagcggttttt aacggagatttaacactaaatcgaagaacttttcccgtttcatttgcgaa tgagctcgttaacaaaatcccccagtttttttatccagctgtaaggattg acattagtaatgaattattgtttggtatatttaaatctgtagttcctttc tgtccgtgtcggcaactgtcgtactcgtgatttacttgtattgacgaata cttactgtagcgcactctgctgctactggtcgtaaggatgtgctatttcg gtgtatggtgggttttttgggggtcggaaccgaagactgttacacgggca cggctcgttgtgtacacgcacagagctcttgcgagtcatgttgtagctag ctcgtcgtgttcaggaactgttcgatggttcggagagagtcgccgcccag aacatacgcgcaccgatgtcagcagacagccttattacaagtatattcaa gcaagtatatccgtagggtgcgggtgatttggatctaaggttcgtactca acactcacgagcagcttgcctatgttacatccttttatcagacataacat aattggagtttacttacacacggggtgtacctgtatgagcaccacctaca attgtagcactggtacttgtacaaagaatttattcgtacgaatcacaggg acggccgccctcaccgaaccagcgaatacctcagcggtcccctgcagtga ctcaacaaagcgatatgaacatcttgcgatggtatcctgctgatagtttt tactgtacaaacacctgtgtagctccttctagcatttttaagttattcac acctcaaggggagggataaattaaataaattccaaaagcgaagatcgaga aactaaattaaaattccaaaaacgaagttggaacacaaccccccgaaaaa aaacaacaaacaaaaaacccaacaaaataaacaaaaacaaaataaatata taactaccagtatctgactaaaagttcaaatactcgtacttacaacaaat agaaatgagccggccaaaattctgcagaaaaaaatttcaaacaagtactg gtataattaaattaaaaaacacatcaaagtatcataacgttagttatttt attttatttaataaaagaaaacaacaagatgggctcaaaactttcaactt atacgatacataccaaataacaatttagtatttatctaagtgcttttcgt agataatggaatacaaatggatatccagagtatacacatggatagtatac actgacacgacaattctgtatctctttatgttaactactgtgaggcatta aatagagcttgatatataaaatgttacatttcacagtctgaacttttgca gattacctaatttggtaagatattaattatgaactgaaagttgatggcat ccctaaatttgatgaaagatgaaattgtaaatgaggtggtaaaagagcta cagtcgttttgttttgagataccatcatctctaacgaaatatctattaaa aatctcagtgtgatcatgagtcattgccatcctggaaaatgtcatcatgg ctgatatttctaactgtttacttgagataaatatatatttacaagaactt cccttgaaattaatttagatataaaatgtttgcgggcaagttactacgag gaataaattatatctagaggttccgcttcctcgctcactgactcgctgcg ctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaa tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagca aaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtt tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaa gtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgg atacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagct cacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggc tgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaa ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcag cagccactggtaacaggattagcagagcgaggtatgtaggcggtgctaca gagttcttgaagtggtggcctaactacggctacactagaaggacagtatt tggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggta gctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtt tgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatccttt gatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag ggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttg gtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatct gtctatttcgttcatccatagttgcctgactccccgtcgtgtagataact acgatacgggagggcttaccatctggccccagtgctgcaatgataccgcg agacccacgctcaccggctccagatttatcagcaataaaccagccagccg gaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccag tctattaattgttgccgggaagctagagtaagtagttcgccagttaatag tttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgt cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtt acatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctcc gatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatgg cagcactgcataattctcttactgtcatgccatccgtaagatgcttttct gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcg accgagttgctcttgcccggcgtcaacacgggataataccgcgccacata gcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaa ctctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcg tgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggt gagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgaca cggaaatgttgaatactcatactcttcctttttcaatattattgaagcat ttatcagggttattgtctcatgagcggatacatatttgaatgtatttaga aaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacct gacgtctaagaaaccattattatcatgacattaacctataaaaataggcg tatcacgaggccctttcgtctggcctaggaagcgacttccaatcgctttg catatccagtaccacacccacaggcgtttgtgctactctactgatagcaa tagatgcgtcataattggttggcccgctgagcctccacaggatactattg cacataccctggtcatgtgcagatcagctcatttgtggagactctggagt aacttagacgacgcctggttcaattgccgcaatgtgcgcccacgcagata atgtattgaggggtggagcgcctcttggggacttgctgtacttgtacggg atattaaacgcactcagcaagaccatgacgtaaaacacacctactgtacg atacgtactgtaggtattgtactcgtacccggtactacaaatagtacgat actatacggagtgtatttgtaccttgatatacgactggcggagtgaagag aaggagttgaacaagaccagatggggatatcagccccagtgctttgtatt acaagtacgagtacttaatagatactgtaaggctattgatacggatggca gtaagtcattgagtaagcaattgtggcccagcatctcccctacgtacttg taccataccccatggagacaccaatggtctttcacgcacactgtcgtgtg ctgtatcgcagaatcgggtgtccaaccaaatgccgttacccccacgtcac agccgatagacagatacaccatcaataccagcaggttgtatcatgcggtt ggctgaaggtaagctgattggtctaaaaactgtagctgtcctaattcaac gagcgctatttggggccaaccacctcggccaagcggcctttaatctgcgt gccccagaggcgtctaatgaggctctggccgccactgtaggagtgtttct ctgtgcgcacacgcagttttgagtttgggcgactttccctttttcccaat tgcgtacacacacagctccgagctaagcgctgtccttgaaccttctccct cttttccctctttttctcttccccttcccctcctccacattaaggccaaa tcctgaattgcaccaactagtacaacgacaacaatggacaagaagtactc catcggtttggacattggtactaactctgtcggctgggccgtcatcaccg acgagtacaaggttccctccaagaagttcaaggtccttggcaacaccgac cgacactctatcaagaagaacctgatcggtgctctgctgttcgactctgg cgagactgccgaggccacccgactgaagcgaaccgctcgacgccgataca cccgacgaaagaaccgaatctgttacctccaggagatcttcagcaacgag atggctaaggtcgacgactccttcttccaccgactcgaggagtctttcct ggtcgaagaggataagaagcacgagcgacaccccatcttcggcaacattg ttgatgaggttgcctaccatgagaagtaccccaccatctaccacctccga aagaagctcgtcgactccactgacaaggctgacctccgactcatctacct tgctctcgcccacatgatcaagttccgaggtcacttcctcattgagggtg atctcaaccccgacaactccgacgttgacaagctgttcatccagctcgtc cagacctacaaccagctctttgaggagaaccctatcaacgcttctggtgt tgacgccaaggccattctctccgcccgactctctaagtcccgacgactcg agaacctcattgcccagctgcccggcgagaagaagaacggcctcttcggt aacctgattgctctctctcttggtctgacccccaacttcaagtccaactt tgacctcgccgaggacgccaagctccagctgtccaaggacacctacgatg acgatctggacaacctcctggcccagatcggtgaccagtacgccgatctc ttccttgccgccaagaacctctccgacgccatcctgctctccgacatcct ccgagtcaacaccgagattaccaaggctcctctgtctgcctctatgatca agcgatacgacgagcaccaccaggatctcactcttctcaaggctctcgtc cgacagcagctccccgagaagtacaaggagattttctttgaccagtccaa gaacggttacgctggctacattgacggtggtgcttcccaggaagagtttt acaagttcatcaagcctattctggagaagatggacggtaccgaggagctg ctcgtcaagctcaaccgagaggacctccttcgaaagcagcgaaccttcga taacggctccatcccccaccagatccacctgggtgagctccacgccattc tccgaagacaagaggacttctaccccttcctaaaggataaccgagagaag atcgagaagattctcaccttccgaatcccctactacgtcggtcccctcgc tcgaggtaactcccgatttgcttggatgacccgaaagtccgaggagacta tcaccccctggaactttgaagaggtagtcgacaagggtgcctccgcccag tctttcattgagcggatgaccaacttcgataagaacctccccaacgagaa ggtccttcccaagcactctctcctctacgagtacttcaccgtctacaacg agctgaccaaggtcaagtacgttaccgagggcatgcgaaagcccgctttc ctctctggtgagcagaagaaggccattgtcgacctcctgttcaagactaa ccgaaaagtcaccgtcaagcagctcaaggaagactacttcaagaagattg agtgcttcgactccgtcgagatttccggtgtcgaggaccgattcaacgcc tccctcggcacctaccacgatcttctgaagatcatcaaggacaaggactt tcttgataacgaggagaacgaggacattctcgaggacatcgtcctcaccc tcacccttttcgaggatcgagagatgatcgaggagcgactcaagacctac gcccatctcttcgacgacaaggtcatgaagcaactcaagcgacgacgata cactggctggggccgactttcccgaaagctcatcaacggcatccgagaca agcagtctggcaagaccatcctggacttcctgaagtccgacggtttcgcc aaccgaaacttcatgcagctcatccacgacgactctcttaccttcaaaga ggatatccagaaggcccaggtttctggccagggcgactccctccacgagc acattgccaacctcgccggatcccccgccatcaaaaagggtatcctccag accgtcaaggttgtcgacgaactcgtgaaggtcatgggccgacacaagcc cgagaacatcgttatcgagatggcccgagagaaccagaccacccagaagg gtcagaagaactcccgagagcgaatgaagcgaatcgaagagggtatcaag gagctcggttcccagattctcaaggagcaccccgtcgagaacacccagct ccagaacgagaaactctacctgtactacctccagaatggccgagacatgt acgttgaccaggagctcgacatcaaccgactctccgactacgacgtcgac cacattgttcctcagtccttcctcaaggacgactccatcgacaacaaggt tctgacccgatctgacaagaaccgaggtaagtccgacaacgttccctccg aagaggtcgttaagaagatgaagaactactggcgacagcttctcaacgcc aaactgatcacccagcgaaagtttgacaacctcaccaaggccgagcgagg tggtctgtccgagctggacaaggccggcttcattaagcgacagctggtcg agactcgacagatcaccaagcacgtcgcccagatcctcgactcccgaatg aacaccaagtacgacgagaacgacaagctcatccgggaggtcaaggtcat caccctgaagtctaagcttgtctccgacttccgaaaggacttccagttct acaaggtccgagagatcaacaactaccaccacgcccacgacgcctacctc aacgccgttgttggtaccgccctcatcaagaagtatcccaagctcgagtc cgagttcgtttacggcgactacaaggtttacgatgtccgaaagatgattg ccaagtccgagcaggagatcggtaaggccaccgccaagtactttttctac tccaacatcatgaatttcttcaagaccgagatcactctcgccaacggtga gattcgaaagcgacccctgattgagactaatggtgagactggtgagatcg tctgggataagggccgagacttcgccaccgtccgaaaggtcctgtccatg ccccaggtcaacattgtcaagaagaccgaggtccagaccggtggcttctc caaggagtccattctccccaagcgaaactccgacaaactcatcgcccgta agaaggactgggatccgaagaagtacggtggtttcgattctcccaccgtt gcctactccgtcctcgttgttgctaaagtcgagaagggtaagtctaagaa actcaagtccgtgaaggagctactcggtatcaccatcatggagcgatctt cttttgagaagaaccccattgacttcctcgaggccaagggttacaaagag gtcaagaaggacctgattatcaagctgcccaagtactccctctttgagct cgagaacggccgaaagcgaatgctggcttccgctggtgagctgcagaagg gcaacgagctcgctctgccctccaagtacgtcaacttcctctacctggcc tcccactacgagaagctcaagggctcccccgaggacaacgagcagaagca gctgttcgttgagcagcacaagcactacctcgacgagatcatcgagcaga tctccgagttctccaagcgagtcatcctcgctgacgccaaccttgataag gttctctctgcttacaacaagcaccgggacaagcccatccgagagcaggc cgagaatatcatccacctcttcactctcaccaacctcggcgctcctgctg ccttcaagtacttcgacaccaccattgaccgaaagaggtacacctccacc aaggaagtcctcgacgccaccctgatccaccagtccatcaccggcctcta cgaaacccgaatcgacctctcccagctcggcggtgactctcgagccgacc ccaagaagaagcgaaaagtctaaatatccgaagatcaagagcgaagcaag ttgtaagtccaggacatgtttcccgcccacgcgagtgatttataacacct ctcttttttgacacccgctcgccttgaaattcatgtcacataaattatag tcaacgacgtttgaataacttgtcttgtagttcgatgatgatcatatgat tacattaatagtaattactgtatttgatatatatactaattacaatagta catattagaacatacaatagttagtgccgtgaagtggcttaaaataccgc gagtcgattacgtaatattattacctcttgcccatcgaacgtacaagtac tcctctgttctctccttcctttgctttgtgcacgaagaactgcggtcagg tgacacaactttttccatctcagggtgtgtcgcgtgtgcttcatccaaac tttagttggggttcgggttcgcgcgagatgatcacgtgccctgatttggt gtcgtcccccgtcgcgctgcgcacgtgatttatttatttccggtggctgc tgtctacgcggggccttctctgcccttctgtttcaaccttcgggcggttc tcgtaaccagcagtagcaatccatttcgaaactcaaagagctaaaaacgt taaacctcagcagtcgctcgacgaatgggctgcggttgggaagcccacga ggcctatagccagagcctcgagttgacaggagcccagacgccttttccaa cggcaacttttatataaaatggcaatgtattcatgcaattgcggccgtgt caggttggagacactggaccacactctccattgcttcctgaggagatgga tcattgctagtgcatctacgcgcagcaatcccgcaagctcgacaaccgta gatgggctttggtgggccaatcaattacgcaacccgcacgttaaattgta tgaggaaggaaggccacggtacaaagtgggtggtcttcacccagtggttg ttggtggcgtcatgcagaccatggccgccagtgtgctggaattgaatatt taccgttcgtataatgtatgctatacgaagttataccggtctcgtagtgt tcacgttcagttcacggtgagcttaaaactatcttcaagaagagatttga gacctgatttatacttgcagcaatgtttacttcttatcgcgatacacgaa tgtgatacggatcaaagtaagcaggactacgataagataacgaatgcggt gcagtccatgtcgattaggtatagatacatttattttgtgttatgttaca ttttggggggatactgtcctacttgtagtacctacttgtagtggcgcgtt aggggcagggcatgctcatgtagagcgcctgccgctcgccgtccgaggcg gtgccgtcgtacagggcggtgtccaggccgcagagggtgaaccccatccg ccggta 10 GCGGAGCCTAGGCCGGCCAGCGTGGCGCGTGGCCATATTGGCCAGCAGCTTACTACAGCTCAGCC AAAGTGGATCATGATGGACGGAAATATCGGACTGGAGGCCAAAAAGGAGGTGCTCAAATACGCAC GGGACAAGTCTGCACAGGTGGCATTCGAACCCACGTCTGTCCCCAAAGCCGCTGCTCTTTCCGAG CTCAATCTGCCCGTTTACCCCAACAACTCCATTGCCTTAGCCACCCCCAACACTGCAGAGCTCGA GGCCATGTTCGAGGCATTCCACGAGAAGGGCCGATTTGACGTTGACGACTGGTTTCCAGTCATAG ACGGTCTTGCTCTCGGAGCTGACTTTAGAAACGGAGCCACCATGTTGTCGCACCAACATCGTGGA CTCAAAGCTATTCTCGAACAAGGAACTCTGGCCCAGGCAATCCACATGCTACCATACATCCCAAC TTTGATCATCAAGGGCGGAGCCAACGGCGTCGTCGTCTTCCAACTCATTGATGATATCGAGTCTG CCATTCATTCACAGCGTTCTGCTTCCAATAAAACGCCTGGCCTGTTCCAGAAGGGCAACGCTGCG AGTGGAAACACCAAGGTCGGCGTCTACATGCAGTACTTTGAGCCCGAAGAAGTGGGCAGTCAGTC GATTGTAAGCGTGACTGGAGCTGGAGACACTCTGTTTGGAACCCTGGCCATGGAGATTGTCAAGG ACGAGTCCTGGTTGAACGATATGGGAGACAAGAAGAGTGCAGTTGTTTCTCGAGCCATGAACAAT GCTGTGAAGACTATTCAGAGTAAGGACGCTGTCTGCAAGAGCATCCTTTAAGTGATTTGCCATGC TTTCTCTTCTTCCACGATGTAAATACTTATTTTACACACTACTGTGCAGTAGCAAATACAGAACA AGAGTTGTCGCCTATTGACAGTACAGTACGAGTAGTGTATGTACAGTAGTTATACAATATCTATG TGAAATTCGTCGGCAGCTTTCGGCTGATGAACTACGAGTTCTTCGTAATAAATCATCAACGTAAT AAGCTTGGTACCAGAGACGGGTTGGCGGCGTATTTGTGTCCCAAAAAACAGCCCCAATTGCCCCA ATTGACCCCAAATTGACCCAGTAGCGGGCCCAACCCCGGCGAGAGCCCCCTTCACCCCACATATC AAACCTCCCCCGGTTCCCACACTTGCCGTTAAGGGCGTAGGGTACTGCAGTCTGGAATCTACGCT TGTTCAGACTTTGTACTAGTTTCTTTGTCTGGCCATCCGGGTAACCCATGCCGGACGCAAAATAG ACTACTGAAAATTTTTTTGCTTTGTGGTTGGGACTTTAGCCAAGGGTATAAAAGACCACCGTCCC CGAATTACCTTTCCTCTTCTTTTCTCTCTCTCCTTGTCAACTCACACCCGAAATCGTTAAGCATT TCCTTCTGAGTATAAGAATCATTCGCTAGCCACAAAAATGGGTGATCTCGATGCCCGAGGAACCT CTGCTCACCCCGAGCTCTCTGAGCGACCTTCTATTATGCCTTCTATGTCTGATATTCAGGACCCT TCTGGTGATGACAAGGCTACTCCCCGAGGATCTGCTGCTGGTCTGCCCCAGCTTGAGCTTGCTGG ACACGCCCGACGACTTGGCCACCTTGAGAACTTCTTTGCTGTCCAGGCTCGACAGCAGATTTACT CTTCTTTTGCTGTTTTTTGTGAGTTTGACACTGCTTGTTCTCTCGCTCAGCTTGCTTCTGCTGTG CGAAACGTTTGTCTTTCTAACCCCCTTCTCCTTCACACTGTTGAGCCTAAGCACCCTGACATCGC TGGATTCTACCACTCTGACGAGTACCTTTCCCGACCTTGGCCTCAGCACGATTACATGCGAGTTC TTCGAGAGGTTCACGTCGCTGACGTTGTTATGAACGGACAGAAGGAGCACGCTCACGTTGTTCGA GATGCTGTTGACGTTTTTCAGGCTCACGGAAACCAGGTTACTTCTGAGCTCCTTGAGCTTATGAC TCAGATTGAGATTCCTCACGCTTCTCAGACTCGACCCTCTTGGCGACTTCTCTGTTTTCCCCACG GAGAGGCTAACCGATGGCGAACCTTTGCTTTTGTTTCTAACCACTGTTCTTCTGATGGTCTTTCT GGTCTTAACTTCTTTCGAGATCTCCAGAAGGAGCTTGCTCACGGCCCCACCTCTGGTGCTCCTGG TGCCCCCGGAGCTTCCGGAGTTATTTTCGATTACGCTCAGGACGCTGCTACCCTGCCCAAGCTGC CCCCTCCCATTGATCAGAAGCTCGATTACCGACCTTCTAAGAAGGCTCTTCTCGGCCTTCTCGCT GGCAAGTTCGTTCGAGAGAAGCTCGGTTACGTTTCTGCTGCTCCTCCCACTACCCCTACCTCTGA CCTTGCTCACCCTGAGGGTCACCAGTACTACTGTTACCTTGTTAACGTTCCCACTTCTTCTGTTG CCCACATTAAGACTCAGGTGCGAGAGAACGTTCCTCACAAGTGTACTCTCACTCCCTTTCTCCAG GCTTGTTGGCTTGTTTCTCTGTTCAAGTACGGTCGAGTTTTTTCTGGTTCTTGGCTTGAGCGATA CACCGATGTTCTTGTTGCTATGAACACTCGACAGCTTCTCCCCGAGGACCTTGAGCTTCAGCGAC AGTACCGATACGGTTCTAACATTGGAGCTGTTCGATACAACTACCCTATTGCTCCCCTTGACGTT CGAGATAACGATCAGAAGTTCTGGTCCCTTGTTGAGTCTTACCGACTTGCCCTTTCTGATGCCCG AGATAAGAACGATTACCTTTACGCTCTTGGTGCTCTTATGCTCCCTGAGATTTACGAGAAGAAGA ACGTTGATGCTGTTGTTAACGATACCATTCTTAACCAGCGACGACAGGGAACCCTTCTTTCTAAC GTTGGTTACGTTCGAGATGAGCAGCCCACTGCTTTTGCTATTAAGAACCACGTTTTTTCTCAGGG AGTTGGAGCTAACCGAAACGCTTTTGTTCTTAACATTTGTGCTACCGATCAGGGTGGTCTTAACA TCGCTATTTCTATTGCTAAGGGAACCCTTGCTTCTCGACAGGAGGGACAGGAGCTTTGTGATATT TTTAAGTCTACTCTCCTTCGATTTTAAACGCGTCTATCCGAAGATCAAGAGCGAAGCAAGTTGTA AGTCCAGGACATGTTTCCCGCCCACGCGAGTGATTTATAACACCTCTCTTTTTTGACACCCGCTC GCCTTGAAATTCATGTCACATAAATTATAGTCAACGACGTTTGAATAACTTGTCTTGTAGTTCGA TGATGATCATATGATTACATTAATAGTAATTACTGTATCTGTACCTGCTGTGGACCACGCACGGC GGAACGTACCGTACAAATATTTTCTTGCTCACATGACTCTCTCTCGGCCGCGCACGCCGGTGGCA AATTGCTCTTGCATTGGCTCTGTCTCTAGACGTCCAAACCGTCCAAAGTGGCAGGGTGACGTGAT GCGACGCACGAAGGAGATGGCCCGGTGGCGAGGAACCGGACACGGCGAGCCGGCGGGAAAAAAGG CGGAAAACGAAAAGCGAAGGGCACAATCTGACGGTGCGGCTGCCACCAACCCAAGGAGGCTATTT TGGGTCGCTTTCCATTTCACATTCGCCCTCAATGGCCACTTTGCGGTGGTGAACATGGTTTCTGA AACAACCCCCCAGAATTAGAGTATATTGATGTGTTTAAGATTGGGTTGCTATTTGGCCATTGTGG GGGAGGGTAGCGACGTGGAGGACATTCCAGGGCGAATTGAGCCTAGAAAGTGGTACCATTCCAAC CGTCTCAGTCGTCCGAATTGATCGCTATAACTATCACCTCTCTCACATGTCTACTTCCCCAACCA ACATCCCCAACCTCCCCCACACTAAAGTTCACGCCAATAATGTAGGCACTCTTTCTGGGTGTGGG ACAGCAGAGCAATACGGAGGGGAGATTACACAACGAGCCACAATTGGGGAGATGGTAGCCATCTC ACTCGACCCGTCGACTTTTGGCAACGCTCAATTACCCACCAAATTTGGGCTGGAGTTGAGGGGAC CGTGTTCCAGCGCTGTAGGACCAGCAACACACACGGTATCAACAGCAACCAACGCCCCCGCTAAT GCACCCAGTACTGCGCAGGTGTGGGCCAGGTGCGTTCCAGATGCGAGTTGGCGAACCCTAAGCCG ACAGTGTACTTTTTGGGACGGGCAGTAGCAATCGTGGGCGTAGACCCCGGTGTATATAAAGGGGT GGAGAGGACGGATTATTAGCACCAACACACACACTTATACTACATGCTAGCCACAAAAATGCTCT CTTTCTTCTGGCGAAACGGTATCGAGACTCCCGAGCCCCTCAAGGCTGACGTTTCCGGCTCTATC CCTCCCTGGCTTCAGGGAACCCTTCTCCGAAACGGTCCTGGTCTGTTCTCCGTTGGCAACACTTC CTACAAGCACTGGTTCGATGGTATGGCTCTCATTCACTCCTTCACCTTTAAGGATGGTGAGGTTT TTTACCGATCTAAGTACCTGAAGTCTGAGACTTACAAGAAGAACATCGCTGCCGACCGAATCGTT GTGTCTGAGTTCGGAACCATGGTGTACCCCGATCCCTGCAAGAACATTTTCTCCCGAGCCTTCTC TTACATGATGAACGCCATTCCTGACTTTACCGATAACAACCTCATTAACATCATTAAGTACGGTG AGGATTACTACGCCTCCTCTGAGGTCAACTACATCAACCAGATTGACCCCCTGACCCTTGAGACT CTCGGACGAACTAACTACCGAAACCACATTGCCATCAACCTTGCCACTGCTCACCCTCACTACGA CGAGGAGGGTAACACTTACAACATGGGCACTGCTATTATGAACCTCGGTCGACCCAAGTACGTGA TTTTCAAGGTGCCCGCCAACACCTCTGATAAGGAGAACAAGAAGCCTGCCCTCTCTGAGGTGGAG CAGGTTTGCTCCATTCCCATCCGACCCTCCCTTTACCCTTCTTACTTCCACTCTTTTGGCATGAC TGAGAACTACATCATCTTCGTTGAGCAGGCCTTCAAGCTGGACATCGTCAAGCTGGCTACTGCTT ACTTCCGAGATATTAACTGGGGATCTTGCCTTAAGTTCGACCAGGATGACATTAACGTGTTCCAC CTGGTCAACAAGAAGACTGGTAAGGCTGTGTCCGTGAAGTACTACACTGACCCCTTTGTTACCTT CCACCACATCAACGCTTACGAGGACGATGGCCACGTCGTCTTCGATCTCATTACTTACAAGGACT CTAAGCTGTACGATATGTTCTACATTCAGAACATGAAGCAGGACGTCAAGCGATTTATTGAGACT AACAAGGACTTCGCTCAGCCCGTGTGCCAGCGATTTGTCCTTCCCGTCAACGTTGATAAGGAGAC TCCTCAGGACATCAACCTTGTCAAGCTGCAGGACACCACTGCCACTGCTGTCCTGAAGGAGGACG GCTCTGTCTACTGCACCCCTGACATCATTTTTAAGGGTCTTGAGCTCCCTGCTATCAACTACAAG TTTAACTCTAAGAAGAACCGATACTTCTACGGCACCCGAGTGGAGTGGTCCCCTTACCCTAACAA GGTCGCTAAGGTGGACGTTGTTACTCGAACCCACAAGATTTGGACTGAGGAGGAGTGTTACCCTT CTGAGCCTGTCTTTATTGCCTCCCCTGACGCCGTTGATGAGGATGACGGTGTGATTCTTTCTTCT GTGGTTTCTTTCAACCCCCAGCGACCCCCTTTCCTGGTTGTCCTCGATGCTAAGTCCTTCAAGGA GATTGCTCGAGCTACCATCGATGCCTCTATTCACATGGACCTTCACGGCCTTTTCATCCACGACA AGTCTACCTAAGTTTTTTGATCAATGATCCAATGGCTTTCACATACCCCCCCACGCCTATAATTA AAACACAGAGAAATATAATCTAACTTAATAAATATTACGGAGAATCTTTCGAGTGTTCAGCAGAA ATATAGCCATTGTAACAAAAGCCGGCTATCGACCGCTTTATCGAAGAATATTTCCCGCCCCCCAG TGGCCAAACGATATCGGTCAGAAGGGGCAGCTCTAAACGAAGAACTGCGGTCAGGTGACACAACT TTTTCCATCTCAGGGTGTGTCGCGTGTGCTTCATCCAAACTTTAGTTGGGGTTCGGGTTCGCGCG AGATGATCACGTGCCCTGATTTGGTGTCGTCCCCCGTCGCGCTGCGCACGTGATTTATTTATTTC CGGTGGCTGCTGTCTACGCGGGGCCTTCTCTGCCCTTCTGTTTCAACCTTCGGGCGGTTCTCGTA ACCAGCAGTAGCAATCCATTTCGAAACTCAAAGAGCTAAAAACGTTAAACCTCAGCAGTCGCTCG ACGAATGGGCTGCGGTTGGGAAGCCCACGAGGCCTATAGCCAGAGCCTCGAGTTGACAGGAGCCC AGACGCCTTTTCCAACGGCAACTTTTATATAAAATGGCAATGTATTCATGCAATTGCGGCCGTGT CAGGTTGGAGACACTGGACCACACTCTCCATTGCTTCCTGAGGAGATGGATCATTGCTAGTGCAT CTACGCGCAGCAATCCCGCAAGCTCGACAACCGTAGATGGGCTTTGGTGGGCCAATCAATTACGC AACCCGCACGTTAAATTGTATGAGGAAGGAAGGCCACGGTACAAAGTGGGTGGTCTTCACCCAGT GGTTGTTGGTGGCGTCATGCAGACCATGCATTGGGGATAGCACAGGGTTGGGGTGTCTTGTGGAC TCAATGGGTGAAAGGAGATGGAAAAGGGCGGTGAAAAGTGGTAGAATCGAAATCCCTGACGTCAA TTTATAAAGTAAAATGCGTTTCTGCCATTTTGCTCCCCTCCTTCTTTCGCAATCGCCTCCCCAAA AGTTGTCGTGGCAGTACACATGCTTGCATACAATGAAGCTAATCCGGCTTGCTCAGTAGTTGCTA TATCCAGGCATGGTGTGAAACCCCTCAAAGTATATATAGGAGCGGTGAGCCCCAGTCTGGGGTCT TTTCTCTCCATCTCAAAACTACTTTCTCACATGCTAGCCACAAAAATGACCACTAAGTACACTTC CGTTCACGAGTCTCCCAACGGCCCTGGTGACGCTCGACCCACCGCTTCCCAGATTATCGACGATT ACAACCTTGAGGGAGAGCTTTCTGGCAAGACTGTTCTCGTCACCGGCTGTTCCTCTGGTATTGGT GTTGAGACTGCCCGAGCTATTTACCGAACTGGTGCCACCCTTTACCTCACTGCCCGAGATGTCGA TAAGGCCAAGACCGTTCTTCCCGACCTTGTTGACACTTCCCGAGTCCACTTTCTCCACCTTGACC TTAACTCTCTGGAGTCTGTTCGAGGTTTTGCTGAGAACTTCAAGTCTAAGTCCACTCAGCTTCAC ATTCTCATCGAGAACGCTGGCGTGATGGCCTGTCCCGAGGGCCGAACCGTCGATGGTTTTGAGAC TCAGTTTGGTATCAACCACCTTGCTCACTTTCTCCTCTTTTACCTCCTCAAGGATACCCTTCTCA ACTCTTCTACCCCCGCTTTCAACTCCCGAGTTGTCATCCTCTCTTCTTGTGCTCACCAGGCTGGT TCCGTTCACCTTAACAACCTGTCTCTTGAGGGTGGATACGAGCCTTGGAAGTCTTACGGCCAGTC CAAGACTGCCAACCTTTGGACTGCCCGAGAGATCGAGAAGCGATTTGGTGCTTCCGGTATCCACT CTTGGGCTGTTCACCCCGGTTCCATCGCTACTGAGCTTCAGCGACACGTTTCCGACGAGCTTAAG CAGAAGTGGGCTGACGATAAGGAGGGTGCCAAGCTGTGGAAGTCCACCGAGCAGGGTGCCGCCAC CACTGTCCTTGCTGCTGTTTCCCCTGAGCTTGAGGGTAAGGGCGGTCTTTACCTTGAGGATACCC AGGTTGCCAAGCCCCCTGCCCGAGGAATGTTTGGTGTTGCTGACTGGGCTTACGATGAGGATGGC CCCTCTAAGCTCTGGGCCAAGTCTCTTGAGCTCCTTAAGCTCCAGTAAAGGTTAGACTATGGATA TGTAATTTAACTGTGTATATAGAGAGCGTGCAAGTATGGAGCGCTTGTTCAGCTTGTATGATGGT CAGACGACCTGTCTGATCGAGTATGTATGATACTGCACAACCTGTGTATCCGCATGATCTGTCCA ATGGGGCATGTTGTTGTGTTTCTCGATACGGAGATGCTGGGTACAAGTAGCTAATACGATTGAAC TACTTATACTTATATGAGGCTTGAAGAAAGCTGACTTGTGTATGACTTATTCTCAACTACATCCC CAGTCACAATACCACCACTGCACTACCACTACACACTAGTGGTGTGTTCTGTGGAGCATTCTCAC TTTTGGTAAACGACATTGCTTCAAGTGCAGCGGAATCAAAAAGTATAAAGTGGGCAGCGAGTATA CCTGTACAGACTGTAGGCGATAACTCAATCCAATTACCCCCCACAACATGACTGGCCAAACTGAT CTCAAGACTTTATTGAAATCAGCAACACCGATTCTCAATGAAGGCACATACTTCTTCTGCAACAT TCACTTGACGCCTAAAGTTGGTGAGAAATGGACCGACAAGACATATTCTGCTATCCACGGACTGT TGCCTGTGTCGGTGGCTACAATACGTGAGTCAGAAGGGCTGACGGTGGTGGTTCCCAAGGAAAAG GTCGACGAGTATCTGTCTGACTCGTCATTGCCGCCTTTGGAGTACGACTCCAACTATGAGTGTGC TTGGATCACTTTGACGATACATTCTTCGTTGGAGGCTGTGGGTCTGACAGCTGCGTTTTCGGCGC GGTTGGCCGACAACAATATCAGCTGCAACGTCATTGCTGGCTTTCATCATGATCACATTTTTGTC GGCAAAGGCGACGCCCAGAGAGCCATTGACGTTCTTTCTAATTTGGACCGATAGCCGTATAGTCC AGTCTATCTATAAGTTCAACTAACTCGTAACTATTACCATAACATATACTTCACTGCCCCAGATA AGGTTCCGATAAAAAGTTCTGCAGACTAAATTTATTTCAGTCTCCTCTTCACCACCAAAATGCCC TCCTACGAAGCTCGAGCTAACGTCCACAAGTCCGCCTTTGCCGCTCGAGTGCTCAAGCTCGTGGC AGCCAAGAAAACCAACCTGTGTGCTTCTCTGGATGTTACCACCACCAAGGAGCTCATTGAGCTTG CCGATAAGGTCGGACCTTATGTGTGCATGATCAAGACCCATATCGACATCATTGACGACTTCACC TACGCCGGCACTGTGCTCCCCCTCAAGGAACTTGCTCTTAAGCACGGTTTCTTCCTGTTCGAGGA CAGAAAGTTCGCAGATATTGGCAACACTGTCAAGCACCAGTACAAGAACGGTGTCTACCGAATCG CCGAGTGGTCCGATATCACCAACGCCCACGGTGTACCCGGAACCGGAATCATTGCTGGCCTGCGA GCTGGTGCCGAGGAAACTGTCTCTGAACAGAAGAAGGAGGACGTCTCTGACTACGAGAACTCCCA GTACAAGGAGTTCCTGGTCCCCTCTCCCAACGAGAAGCTGGCCAGAGGTCTGCTCATGCTGGCCG AGCTGTCTTGCAAGGGCTCTCTGGCCACTGGCGAGTACTCCAAGCAGACCATTGAGCTTGCCCGA TCCGACCCCGAGTTTGTGGTTGGCTTCATTGCCCAGAACCGACCTAAGGGCGACTCTGAGGACTG GCTTATTCTGACCCCCGGGGTGGGTCTTGACGACAAGGGAGACGCTCTCGGACAGCAGTACCGAA CTGTTGAGGATGTCATGTCTACCGGAACGGATATCATAATTGTCGGCCGAGGTCTGTACGGCCAG AACCGAGATCCTATTGAGGAGGCCAAGCGATACCAGAAGGCTGGCTGGGAGGCTTACCAGAAGAT TAACTGTTAGAGGTTAGACTATGGATATGTAATTTAACTGTGTATATAGAGAGCGTGCAAGTATG GAGCGCTTGTTCAGCTTGTATGATGGTCAGACGACCTGTCTGATCGAGTATGTATGATACTGCAC AACCTGTGTATCCGCATGATCTGTCCAATGGGGCATGTTGTTGTGTTTCTCGATACGGAGATGCT GGGTACAAGTAGCTAATACGATTGAACTACTTATACTTATATGAGGCTTGAAGAAAGCTGACTTG TGTATGACTTATTCTCAACTACATCCCCAGTCACAATACCACCACTGCACTACCACTACACCTCG AGCATGCACACTATTATCACATTACTACATCCAAACCCCTACAGGGGGAGGAGCTCTCCAATCAA ATATGTACATTAACTATCTCTCGTAAATCATTGTTATAAGACCGTCCGTGACTGTCTAAATCGGT TCATTCGTTGTAACAAATCAGTGTAACAACTCGTGGTACGCCCGGTTTGCTTCGCTCACGCTCCC CTAGATTTTCCGTCTAGGACACAACAAGCCCTGGTGGATGACGTTCAACGCCTTCAGCGCGTCTT TTTCCTCAATAACACACGAGATGTTAATTTCGTTGGCTCCCTGAGAAATCATCTCAATGTTAATA CCTGCCTGGGCCAGAGTAGAGAAGAACATGCCTGCACATCCGACCATGGCCTTCATTCTCGTTCC GATCAGCGACACAATGGTCATGCCTCGCTTCACATCTACTGTGCCGTACTTTCGCAGCTCCTCCA CAGCCTGCTTGAGGTTGGAGTCGGGAGCATGGAAGGCCATCGACACATGGACCTCAGAAGTGGAG ATGAGATCGACGACAAGTTTCTGCTGGTCGAGAGTAGCAAAGATCTTGTTCAAGAAGCCATGACT CTTGGTTCGCTTATTCGAATGAACGTTGAGAACGGTGATATTCGATTTGGTAGTGACAGCTGTAG GCTTCTTCTCGTCATCTACAGGAGCAGACACCTGGGGAGTGAATGAGCCAGAGGACAGAGAAGTT AGCGAGTCGGTGGAAGAAGCCAGATCCTGGACATTGTTTTTCGTTTTCAGGTTGCCCGAGCCGTC AGCAGATGGGTAGATGATAGTTCCTCCTCCCAGGGGGTTTTCCACGTTTTTGATTCGGATAGGAA TATGGGCCTTAATGACCTGCTCCATGGTGAAGGGGTGGATGACTTCAGATCCGTAGTAGGTCAAC TCAGCGGCCTCCTCGGGGGTAATGATGGGCAGCAGACGGGCAGTGGACACCTTTCTGGGATCGGC AGTGAAGACACCATCGACTTCTTTCCAGATCTGAAGCTCCTTGGCGTCCAGACCCACAGCCACCA GAGCAGCACACAGATCGGTGTATCCTCGTCCGATCTGGCTTAGGAGGCCTCCCTTGACGGGGCCA AAGAAACCGGTCAACACGGGCACCATGTTTCCCTTGACAGGAGCAGAGTCCGTGTTAGGAGAGCC GGGAAGAGTGATGATCTCGCCCAGAACACGTCCGAGGTCCGAATAGAACTTGGGATCTTCGGCAT TGGTGGTGGTAACTGCGTGAGACAGGTCAAAGTACCGTGCGTTAACACCAGCGTCCCGCATAACT GCAGTCATGTACATGCAACTGAGCTTCTCCCCAATGGCCATGATGGAGTCGAGTGTTCGGGGGGA GATCTCAGAAATGATTTCAGCAGCAGCCAGGATTCGCAACAGCTGGTCGCACTCGCCGTTGATGT CGGCATTGAGGTTTTCGAGCAGTTCGGGGTTCTTGACGTCACGCTTAGCAGCTGCAAGATGGTCT TCTCGGATAGACTCAATGATGGGGTTGTAGGCGTCTGATCCGAGAAGAGCAGAGTCAGCAGCAGC AATAAGACGGGTGGTGGTTCCCTCGGCCTTGGTGGCCTCTAGATGGCCTCCTTGGCCCCATTCCA GCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTT CCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCA GCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATAC CAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGC AGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT GGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTT ACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTT TTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTT CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCA AAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATA TGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTC TATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTA CCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAGCCACGCTCACCGGCTCCAGATTTATCAGC AATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCC AGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTT GTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGG TTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCG GTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTG CATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAA GTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATA CCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTC TCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTC AGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAA AGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGC ATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAAT AGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGA CATTAACCTATAAAAATAGGCGTATCACGAG 11 GGTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGC TCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTC CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACT ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGT AGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCA GCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCT GAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCT TTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCAT GAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCT AAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCA GCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACG GGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAG ATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCC GCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT GCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCAT TCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTT AGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTAT GGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGT ACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACA CGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGG GCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCA ACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAAT GCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATA TTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAA ATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATT ATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTGGCCTAGGAAGC GACTTCCAATCGCTTTGCATATCCAGTACCACACCCACAGGCGTTTGTGCTACTCTACTGATAGC AATAGATGCGTCATAATTGGTTGGCCCGCTGAGCCTCCACAGGATACTATTGCACATACCCTGGT CATGTGCAGATCAGCTCATTTGTGGAGACTCTGGAGTAACTTAGACGACGCCTGGTTCAATTGCC GCAATGTGCGCCCACGCAGATAATGTATTGAGGGGTGGAGCGCCTCTTGGGGACTTGCTGTACTT GTACGGGATATTAAACGCACTCAGCAAGACCATGACGTAAAACACACCTACTGTACGATACGTAC TGTAGGTATTGTACTCGTACCCGGTACTACAAATAGTACGATACTATACGGAGTGTATTTGTACC TTGATATACGACTGGCGGAGTGAAGAGAAGGAGTTGAACAAGACCAGATGGGGATATCAGCCCCA GTGCTTTGTATTACAAGTACGAGTACTTAATAGATACTGTAAGGCTATTGATACGGATGGCAGTA AGTCATTGAGTAAGCAATTGTGGCCCAGCATCTCCCCTACGTACTTGTACCATACCCCATGGAGA CACCAATGGTCTTTCACGCACACTGTCGTGTGCTGTATCGCAGAATCGGGTGTCCAACCAAATGC CGTTACCCCCACGTCACAGCCGATAGACAGATACACCATCAATACCAGCAGGTTGTATCATGCGG TTGGCTGAAGGTAAGCTGATTGGTCTAAAAACTGTAGCTGTCCTAATTCAACGAGCGCTATTTGG GGCCAACCACCTCGGCCAAGCGGCCTTTAATCTGCGTGCCCCAGAGGCGTCTAATGAGGCTCTGG CCGCCACTGTAGGAGTGTTTCTCTGTGCGCACACGCAGTTTTGAGTTTGGGCGACTTTCCCTTTT TCCCAATTGCGTACACACACAGCTCCGAGCTAAGCGCTGTCCTTGAACCTTCTCCCTCTTTTCCC TCTTTTTCTCTTCCCCTTCCCCTCCTCCACATTAAGGCCAAATCCTGAATTGCACCAACTAGTAC AACGACAACAATGGACAAGAAGTACTCCATCGGTTTGGACATTGGTACTAACTCTGTCGGCTGGG CCGTCATCACCGACGAGTACAAGGTTCCCTCCAAGAAGTTCAAGGTCCTTGGCAACACCGACCGA CACTCTATCAAGAAGAACCTGATCGGTGCTCTGCTGTTCGACTCTGGCGAGACTGCCGAGGCCAC CCGACTGAAGCGAACCGCTCGACGCCGATACACCCGACGAAAGAACCGAATCTGTTACCTCCAGG AGATCTTCAGCAACGAGATGGCTAAGGTCGACGACTCCTTCTTCCACCGACTCGAGGAGTCTTTC CTGGTCGAAGAGGATAAGAAGCACGAGCGACACCCCATCTTCGGCAACATTGTTGATGAGGTTGC CTACCATGAGAAGTACCCCACCATCTACCACCTCCGAAAGAAGCTCGTCGACTCCACTGACAAGG CTGACCTCCGACTCATCTACCTTGCTCTCGCCCACATGATCAAGTTCCGAGGTCACTTCCTCATT GAGGGTGATCTCAACCCCGACAACTCCGACGTTGACAAGCTGTTCATCCAGCTCGTCCAGACCTA CAACCAGCTCTTTGAGGAGAACCCTATCAACGCTTCTGGTGTTGACGCCAAGGCCATTCTCTCCG CCCGACTCTCTAAGTCCCGACGACTCGAGAACCTCATTGCCCAGCTGCCCGGCGAGAAGAAGAAC GGCCTCTTCGGTAACCTGATTGCTCTCTCTCTTGGTCTGACCCCCAACTTCAAGTCCAACTTTGA CCTCGCCGAGGACGCCAAGCTCCAGCTGTCCAAGGACACCTACGATGACGATCTGGACAACCTCC TGGCCCAGATCGGTGACCAGTACGCCGATCTCTTCCTTGCCGCCAAGAACCTCTCCGACGCCATC CTGCTCTCCGACATCCTCCGAGTCAACACCGAGATTACCAAGGCTCCTCTGTCTGCCTCTATGAT CAAGCGATACGACGAGCACCACCAGGATCTCACTCTTCTCAAGGCTCTCGTCCGACAGCAGCTCC CCGAGAAGTACAAGGAGATTTTCTTTGACCAGTCCAAGAACGGTTACGCTGGCTACATTGACGGT GGTGCTTCCCAGGAAGAGTTTTACAAGTTCATCAAGCCTATTCTGGAGAAGATGGACGGTACCGA GGAGCTGCTCGTCAAGCTCAACCGAGAGGACCTCCTTCGAAAGCAGCGAACCTTCGATAACGGCT CCATCCCCCACCAGATCCACCTGGGTGAGCTCCACGCCATTCTCCGAAGACAAGAGGACTTCTAC CCCTTCCTAAAGGATAACCGAGAGAAGATCGAGAAGATTCTCACCTTCCGAATCCCCTACTACGT CGGTCCCCTCGCTCGAGGTAACTCCCGATTTGCTTGGATGACCCGAAAGTCCGAGGAGACTATCA CCCCCTGGAACTTTGAAGAGGTAGTCGACAAGGGTGCCTCCGCCCAGTCTTTCATTGAGCGGATG ACCAACTTCGATAAGAACCTCCCCAACGAGAAGGTCCTTCCCAAGCACTCTCTCCTCTACGAGTA CTTCACCGTCTACAACGAGCTGACCAAGGTCAAGTACGTTACCGAGGGCATGCGAAAGCCCGCTT TCCTCTCTGGTGAGCAGAAGAAGGCCATTGTCGACCTCCTGTTCAAGACTAACCGAAAAGTCACC GTCAAGCAGCTCAAGGAAGACTACTTCAAGAAGATTGAGTGCTTCGACTCCGTCGAGATTTCCGG TGTCGAGGACCGATTCAACGCCTCCCTCGGCACCTACCACGATCTTCTGAAGATCATCAAGGACA AGGACTTTCTTGATAACGAGGAGAACGAGGACATTCTCGAGGACATCGTCCTCACCCTCACCCTT TTCGAGGATCGAGAGATGATCGAGGAGCGACTCAAGACCTACGCCCATCTCTTCGACGACAAGGT CATGAAGCAACTCAAGCGACGACGATACACTGGCTGGGGCCGACTTTCCCGAAAGCTCATCAACG GCATCCGAGACAAGCAGTCTGGCAAGACCATCCTGGACTTCCTGAAGTCCGACGGTTTCGCCAAC CGAAACTTCATGCAGCTCATCCACGACGACTCTCTTACCTTCAAAGAGGATATCCAGAAGGCCCA GGTTTCTGGCCAGGGCGACTCCCTCCACGAGCACATTGCCAACCTCGCCGGATCCCCCGCCATCA AAAAGGGTATCCTCCAGACCGTCAAGGTTGTCGACGAACTCGTGAAGGTCATGGGCCGACACAAG CCCGAGAACATCGTTATCGAGATGGCCCGAGAGAACCAGACCACCCAGAAGGGTCAGAAGAACTC CCGAGAGCGAATGAAGCGAATCGAAGAGGGTATCAAGGAGCTCGGTTCCCAGATTCTCAAGGAGC ACCCCGTCGAGAACACCCAGCTCCAGAACGAGAAACTCTACCTGTACTACCTCCAGAATGGCCGA GACATGTACGTTGACCAGGAGCTCGACATCAACCGACTCTCCGACTACGACGTCGACCACATTGT TCCTCAGTCCTTCCTCAAGGACGACTCCATCGACAACAAGGTTCTGACCCGATCTGACAAGAACC GAGGTAAGTCCGACAACGTTCCCTCCGAAGAGGTCGTTAAGAAGATGAAGAACTACTGGCGACAG CTTCTCAACGCCAAACTGATCACCCAGCGAAAGTTTGACAACCTCACCAAGGCCGAGCGAGGTGG TCTGTCCGAGCTGGACAAGGCCGGCTTCATTAAGCGACAGCTGGTCGAGACTCGACAGATCACCA AGCACGTCGCCCAGATCCTCGACTCCCGAATGAACACCAAGTACGACGAGAACGACAAGCTCATC CGGGAGGTCAAGGTCATCACCCTGAAGTCTAAGCTTGTCTCCGACTTCCGAAAGGACTTCCAGTT CTACAAGGTCCGAGAGATCAACAACTACCACCACGCCCACGACGCCTACCTCAACGCCGTTGTTG GTACCGCCCTCATCAAGAAGTATCCCAAGCTCGAGTCCGAGTTCGTTTACGGCGACTACAAGGTT TACGATGTCCGAAAGATGATTGCCAAGTCCGAGCAGGAGATCGGTAAGGCCACCGCCAAGTACTT TTTCTACTCCAACATCATGAATTTCTTCAAGACCGAGATCACTCTCGCCAACGGTGAGATTCGAA AGCGACCCCTGATTGAGACTAATGGTGAGACTGGTGAGATCGTCTGGGATAAGGGCCGAGACTTC GCCACCGTCCGAAAGGTCCTGTCCATGCCCCAGGTCAACATTGTCAAGAAGACCGAGGTCCAGAC CGGTGGCTTCTCCAAGGAGTCCATTCTCCCCAAGCGAAACTCCGACAAACTCATCGCCCGTAAGA AGGACTGGGATCCGAAGAAGTACGGTGGTTTCGATTCTCCCACCGTTGCCTACTCCGTCCTCGTT GTTGCTAAAGTCGAGAAGGGTAAGTCTAAGAAACTCAAGTCCGTGAAGGAGCTACTCGGTATCAC CATCATGGAGCGATCTTCTTTTGAGAAGAACCCCATTGACTTCCTCGAGGCCAAGGGTTACAAAG AGGTCAAGAAGGACCTGATTATCAAGCTGCCCAAGTACTCCCTCTTTGAGCTCGAGAACGGCCGA AAGCGAATGCTGGCTTCCGCTGGTGAGCTGCAGAAGGGCAACGAGCTCGCTCTGCCCTCCAAGTA CGTCAACTTCCTCTACCTGGCCTCCCACTACGAGAAGCTCAAGGGCTCCCCCGAGGACAACGAGC AGAAGCAGCTGTTCGTTGAGCAGCACAAGCACTACCTCGACGAGATCATCGAGCAGATCTCCGAG TTCTCCAAGCGAGTCATCCTCGCTGACGCCAACCTTGATAAGGTTCTCTCTGCTTACAACAAGCA CCGGGACAAGCCCATCCGAGAGCAGGCCGAGAATATCATCCACCTCTTCACTCTCACCAACCTCG GCGCTCCTGCTGCCTTCAAGTACTTCGACACCACCATTGACCGAAAGAGGTACACCTCCACCAAG GAAGTCCTCGACGCCACCCTGATCCACCAGTCCATCACCGGCCTCTACGAAACCCGAATCGACCT CTCCCAGCTCGGCGGTGACTCTCGAGCCGACCCCAAGAAGAAGCGAAAAGTCTAAATATCCGAAG ATCAAGAGCGAAGCAAGTTGTAAGTCCAGGACATGTTTCCCGCCCACGCGAGTGATTTATAACAC CTCTCTTTTTTGACACCCGCTCGCCTTGAAATTCATGTCACATAAATTATAGTCAACGACGTTTG AATAACTTGTCTTGTAGTTCGATGATGATCATATGATTACATTAATAGTAATTACTGTATTTGAT ATATATACTAATTACAATAGTACATATTAGAACATACAATAGTTAGTGCCGTGAAGTGGCTTAAA ATACCGCGAGTCGATTACGTAATATTATTACCTCTTGCCCATCGAACGTACAAGTACTCCTCTGT TCTCTCCTTCCTTTGCTTTGTGCACGAAGAACTGCGGTCAGGTGACACAACTTTTTCCATCTCAG GGTGTGTCGCGTGTGCTTCATCCAAACTTTAGTTGGGGTTCGGGTTCGCGCGAGATGATCACGTG CCCTGATTTGGTGTCGTCCCCCGTCGCGCTGCGCACGTGATTTATTTATTTCCGGTGGCTGCTGT CTACGCGGGGCCTTCTCTGCCCTTCTGTTTCAACCTTCGGGCGGTTCTCGTAACCAGCAGTAGCA ATCCATTTCGAAACTCAAAGAGCTAAAAACGTTAAACCTCAGCAGTCGCTCGACGAATGGGCTGC GGTTGGGAAGCCCACGAGGCCTATAGCCAGAGCCTCGAGTTGACAGGAGCCCAGACGCCTTTTCC AACGGCAACTTTTATATAAAATGGCAATGTATTCATGCAATTGCGGCCGTGTCAGGTTGGAGACA CTGGACCACACTCTCCATTGCTTCCTGAGGAGATGGATCATTGCTAGTGCATCTACGCGCAGCAA TCCCGCAAGCTCGACAACCGTAGATGGGCTTTGGTGGGCCAATCAATTACGCAACCCGCACGTTA AATTGTATGAGGAAGGAAGGCCACGGTACAAAGTGGGTGGTCTTCACCCAGTGGTTGTTGGTGGC GTCATGCAGACCATGCATTGGGGATAGCACAGGGTTGGGGTGTCTTGTGGACTCAATGGGTGAAA GGAGATGGAAAAGGGCGGTGAAAAGTGGTAGAATCGAAATCCCTGACGTCAATTTATAAAGTAAA ATGCGTTTCTGCCATTTTGCTCCCCTCCTTCTTTCGCAATCGCCTCCCCAAAAGTTGTCGTGGCA GTACACATGCTTGCATACAATGAAGCTAATCCGGCTTGCTCAGTAGTTGCTATATCCAGGCATGG TGTGAAACCCCTCAAAGTATATATAGGAGCGGTGAGCCCCAGTCTGGGGTCTTTTCTCTCCATCT CAAAACTACTTTCTCACAATGACGAGACTGATGAGTCCGTGAGGACGAAACGAGTAAGCTCGTCG AGGGCTGCAAAATTCACGAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTAT CAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTGGCCGGCATGGTCCCAGCCTCCTCGCTGGCG CCGGCTGGGCAACATGCTTCGGCATGGCGAATGGGACTAAACTTCGAGCTAATCCAGTAGCTTAC GTTACCCAGGGGCAGGTCAACTGGCTAGCCACGAGTCTGTCCCAGGTCGCAATTTAGTGTAATAA ACAATATATATATTGAGTCTAAAGGGAATTGTAGCTATTGTGATTGTGTGATTTTCGTCTTGCTG GTTCTTATTGTGTCCCATTCGTTTCATCCTGATGAGGACCCCTGGAACCGGTGTTTTCTTAGTCT CTGCAATCGCTAGTCTTGTTGCTATGACAGTTGCGTCGACACTATTCAGGTCATCTATCGGTTAT TCTGATATTATAATACCTCCGGATCGATGTACCTGATTTATACTTGCAGCAATGTTTACTTCTTA TCGTTGGACCCCGTCTTCAATTACACTTCCCAACTGGGAACACCCCTCTTTATCGACCCATTTTA GGTAATTTACCCTAGCCCATTGTCTCCATAAGGAATATTACCCTAACCCACAGTCCAGGGTGCCC AGGTCCTTCTTTGGCCAAATTTTAACTTCGGTCCTATGGCACAGCGGTAGCGCGTGAGATTGCAA ATCTTAAGGTCCCGAGTTCGAATCTCGGTGGGACCTAGTTGAAAAATACCTCTAATGCGCCGATG GTTTAGTGGTAAAATCCATCGTTGCCATCGATGGGCCCCCGGTTCGATTCCGGGTCGGCGCAGGT TGACGTACAGCAGGCTGAACGAGGATGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGT CCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTTTTCGCGATACACGAATGTGA TACGGATCAAAGTAAGCAGGACTACGATAAGATAACGAATGCGGTGCAGTCCATGTCGATTAGGT ATAGATACATTTATTTTGTGTTATGTTACATTTTGGGGGGATACTGTCCTACTTGTAGTACCTAC TTGTAGTGGCGCGTCTATTCCTTTGCCCTCGGACGAGTGCTGGGGCGTCGGTTTCCACTATCGGC GAGTACTTCTACACAGCCATCGGTCCAGACGGCCGCGCTTCTGCGGGCGATTTGTGTACGCCCGA CAGTCCCGGCTCCGGATCGGACGATTGCGTCGCATCGACCCTGCGCCCAAGCTGCATCATCGAAA TTGCCGTCAACCAAGCTCTGATAGAGTTGGTCAAGACCAATGCGGAGCATATACGCCCGGAGCCG CGGCGATCCTGCAAGCTCCGGATGCCTCCGCTCGAAGTAGCGCGTCTGCTGCTCCATACAAGCCA ACCACGGCCTCCAGAAGAAGATGTTGGCGACCTCGTATTGGGAATCCCCGAACATCGCCTCGCTC CAGTCAATGACCGCTGTTATGCGGCCATTGTCCGTCAGGACATTGTTGGAGCCGAAATCCGCGTG CACGAGGTGCCGGACTTCGGGGCAGTCCTCGGCCCAAAGCATCAGCTCATCGAGAGCCTGCGCGA CGGACGCACTGACGGTGTCGTCCATCACAGTTTGCCAGTGATACACATGGGGATCAGCAATCGCG CATATGAAATCACGCCATGTAGTGTATTGACCGATTCCTTGCGGTCCGAATGGGCCGAACCCGCT CGTCTGGCTAAGATCGGCCGCAGCGATCGCATCCATGGCCTCCGCGACCGGCTGCAGAACAGCGG GCAGTTCGGTTTCAGGCAGGTCTTGCAACGTGACACCCTGTGCACGGCGGGAGATGCAATAGGTC AGGCTCTCGCTGAATTCCCCAATGTCAAGCACTTCCGGAATCGGGAGCGCGGCCGATGCAAAGTG CCGATAAACATAACGATCTTTGTAGAAACCATCGGCGCAGCTATTTACCCGCAGGACATATCCAC GCCCTCCTACATCGAAGCTGAAAGCACGAGATTCTTCGCCCTCCGAGAGCTGCATCAGGTCGGAG ACGCTGTCGAACTTTTCGATCAGAAACTTCTCGACAGACGTCGCGGTGAGTTCAGGCTTTTTCAT ATGGGTACCTGAGAACATTTTTGTGTCTAGGTGTTTGTGTTTGGACTGCGATCAGTGAAGAAAAG AAGAGGAAAAATTGTGCAAGAAATTTTGCTTTCAAGACTTGGCTGATGCAGCAGGGTAACTCTGG GACACAGACCTATGTTTGTGGTTAAACTCAATGCACGTGGTACGTGCGTGGAGCGCTTACCCATC CAAGGGTGTGGACATGGAACCGACGGTCCGTGGAGTTGTGTAATGTCATTTTGGCGACTCTTGAA GCAAGGCTATAAAAAAATTGTGTGGCTTGAGTCTTATCGAGCTCGGTCACTACAAGAGTTAATCT TCCTGTCTCAGGCAGACAGGTCAGGCAGGGTTACTTTTGGGTGTGCTGTAACTCACTGTATGGCC GTTAGTGCGCATAGACGTTGTACATACTGGACCGAATTGTAGCGTGCTCAATAGGGCCAATAAAG CTATTGTAGGGATCCGAATTTTCAGAACCTAATTTATCTGTTACCCGGCCTGTGGCTCGCACAGC TTAAAAATGGTCAAACTTTCCCCTTCTTGTCTTTTTTTCCTCACATTCATCAGGTTCTTGTCTTG ATCTTTCAAGTGAGTATTAATTACCGACCTTGGTTCTTCATTGGGAGAGCATTGGAAGCCGTGGT GCAGCAACCACAAAACGGTTCTTCCCCTTCGATACCTTCTTGCCTGCCTTTCAATACAAGTCGGC TCGATTAGCGGTGGTCGCCCCCGCCAGCGGAGAACATGGAACTAACCCAGAATGAGAGCTAAGTG GAGAAAGAAGAGAGTCAGACGACTCAAGCGAAAGCGCCGCAAGGTCCGAGCTCGATCCAAATAAG CGGTTTTTAACGGAGATTTAACACTAAATCGAAGAACTTTTCCCGTTTCATTTGCGAATGAGCTC GTTAACAAAATCCCCCAGTTTTTTTATCCAGCTGTAAGGATTGACATTAGTAATGAATTATTGTT TGGTATATTTAAATCTGTAGTTCCTTTCTGTCCGTGTCGGCAACTGTCGTACTCGTGATTTACTT GTATTGACGAATACTTACTGTAGCGCACTCTGCTGCTACTGGTCGTAAGGATGTGCTATTTCGGT GTATGGTGGGTTTTTTGGGGGTCGGAACCGAAGACTGTTACACGGGCACGGCTCGTTGTGTACAC GCACAGAGCTCTTGCGAGTCATGTTGTAGCTAGCTCGTCGTGTTCAGGAACTGTTCGATGGTTCG GAGAGAGTCGCCGCCCAGAACATACGCGCACCGATGTCAGCAGACAGCCTTATTACAAGTATATT CAAGCAAGTATATCCGTAGGGTGCGGGTGATTTGGATCTAAGGTTCGTACTCAACACTCACGAGC AGCTTGCCTATGTTACATCCTTTTATCAGACATAACATAATTGGAGTTTACTTACACACGGGGTG TACCTGTATGAGCACCACCTACAATTGTAGCACTGGTACTTGTACAAAGAATTTATTCGTACGAA TCACAGGGACGGCCGCCCTCACCGAACCAGCGAATACCTCAGCGGTCCCCTGCAGTGACTCAACA AAGCGATATGAACATCTTGCGATGGTATCCTGCTGATAGTTTTTACTGTACAAACACCTGTGTAG CTCCTTCTAGCATTTTTAAGTTATTCACACCTCAAGGGGAGGGATAAATTAAATAAATTCCAAAA GCGAAGATCGAGAAACTAAATTAAAATTCCAAAAACGAAGTTGGAACACAACCCCCCGAAAAAAA ACAACAAACAAAAAACCCAACAAAATAAACAAAAACAAAATAAATATATAACTACCAGTATCTGA CTAAAAGTTCAAATACTCGTACTTACAACAAATAGAAATGAGCCGGCCAAAATTCTGCAGAAAAA AATTTCAAACAAGTACTGGTATAATTAAATTAAAAAACACATCAAAGTATCATAACGTTAGTTAT TTTATTTTATTTAATAAAAGAAAACAACAAGATGGGCTCAAAACTTTCAACTTATACGATACATA CCAAATAACAATTTAGTATTTATCTAAGTGCTTTTCGTAGATAATGGAATACAAATGGATATCCA GAGTATACACATGGATAGTATACACTGACACGACAATTCTGTATCTCTTTATGTTAACTACTGTG AGGCATTAAATAGAGCTTGATATATAAAATGTTACATTTCACAGTCTGAACTTTTGCAGATTACC TAATTTGGTAAGATATTAATTATGAACTGAAAGTTGATGGCATCCCTAAATTTGATGAAAGATGA AATTGTAAATGAGGTGGTAAAAGAGCTACAGTCGTTTTGTTTTGAGATACCATCATCTCTAACGA AATATCTATTAAAAATCTCAGTGTGATCATGAGTCATTGCCATCCTGGAAAATGTCATCATGGCT GATATTTCTAACTGTTTACTTGAGATAAATATATATTTACAAGAACTTCCCTTGAAATTAATTTA GATATAAAATGTTTGCGGGCAAGTTACTACGAGGAATAAATTATATCTAGA 12 MGDLDARGTSAHPELSERPSIMPSMSDIQDPSGDDKATPRGSAAGLPQLELAGHARRLG HLENFFAVQHRQQIYSSFAVFCEFDTACSLAQLASAVRNVCLSNPLLLHTVEPKHPDIA GFYHSDEYLSRPWPQHDYMRVLREVHVADVVMNGQKEHAHVVRDAVDVFQAHGNQVTSE LLELMTQIEIPHASQTRPSWRLLCFPHGEANRWRTFAFVSNHCSSDGLSGLNFERDLQK ELAHGPTSGAPGAPGASGVIFDYAQDAATLPKLPPPIDQKLDYRPSKKALLGLLAGKFV REKLGYVSAAPPTTPTSDLAHPEGHQYYCYLVNVPTSSVAHIKTQVRENVPHKCTLTPF LQACWLVSLFKYGRVESGSWLERYTDVLVAMNTRQLLPEDLELQRQYRYGSNVGGVRYN YPIAPLDVRDNDQKFWSLVESYRLALSDARDKNDYLYALGALMLPEIYEKKNVDAVVND TILNQRRSGTLISNVGYVRDEQPTAFAIKNHVFSQGVGANRNAFVLNICATDQGGLNIA ISIAKGTLASRQEGQELCDIFKSTLLRF