Chimeric polypeptides

Abstract

Chimeric clostridial neurotoxins in which the activation loop has been replaced by an activation loop from a different subtype within the same serotype. Methods of producing, activating, and using such neurotoxins. Compositions comprising such neurotoxins. Polynucleotides, vectors and cells for expressing such neurotoxins.

Claims

1. A chimeric polypeptide comprising: (a) an LH.sub.N backbone from a BoNT/F.sub.2 subtype; and (b) an activation loop region from a BoNT/F.sub.1 subtype; wherein the LH.sub.N backbone comprises both: (i) a light chain domain of the BoNT/F.sub.7 subtype; and (ii) a H.sub.N domain of the BoNT/F.sub.7 subtype.

2. The polypeptide of claim 1, wherein the activation loop comprises the amino acid sequence of SEQ ID NO: 66.

3. The polypeptide of claim 1, wherein a cleavage site within the activation loop is specifically recognized and capable of being cleaved by an endopeptidase.

4. The polypeptide of claim 3, wherein cleavage thereof by the endopeptidase results in the formation of an active holotoxin.

5. The polypeptide of claim 1, further comprising a targeting moiety capable of binding directly to a cell or to a ligand or ligands that bind to a cell.

6. The polypeptide of claim 5, wherein the targeting moiety is from a clostridial neurotoxin.

7. The polypeptide of claim 6, wherein the targeting moiety is a non-clostridial targeting moiety.

8. A polynucleotide encoding the polypeptide of claim 1.

9. A method of producing the polypeptide of claim 1, comprising expressing a polynucleotide encoding the polypeptide in a cell, and recovering the expressed polypeptide.

10. A method of activating the polypeptide of claim 1, comprising incubating the polypeptide with an endopeptidase.

11. A method of preventing or treating a disease or disorder for which a therapy with a botulinum neurotoxin is indicated, the method comprising administering a composition comprising the polypeptide of claim 1 to a patient in need thereof.

12. A method of preventing or alleviating a cosmetic indication for which the application of a botulinum neurotoxin is indicated, the method comprising administering the composition comprising the polypeptide of claim 1 to a subject in need thereof.

Description

DESCRIPTION OF FIGURES

(1) FIG. 1: Alignment of the activation loop sequences of all BoNT/A subtypes and all BoNT/F subtypes. The activation sequence for each subtype is underlined. The dotted lines within the activation loops correspond to gaps introduced as part of the alignment process to facilitate alignment between the different BoNT/A and BoNT/F subtypes.

(2) FIG. 2: Phylogenetic analysis of BoNT/F protein sequences based on sequence alignment.

(3) FIG. 3: Table showing the percentage sequence identity and percentage sequence divergence between individual BoNT/F sequences.

(4) FIG. 4: Alignment of all non-redundant BoNT/F amino acid sequences identified in a UniParc BLAST. The light chain is shown in bold, the activation loop is double-underlined, the belt sequence is dotted underlined, the H.sub.N domain is italicised, the H.sub.CN domain is single underlined and the H.sub.CC domain is dashed underlined. Dotted lines within individual BoNT/F sequences correspond to gaps introduced as part of the alignment process to facilitate alignment between the different BoNT/F subtypes.

(5) FIG. 5: Schematic of rBoNT/F.sub.1, rBoNTF.sub.7 and rBoNTF.sub.7-1

(6) FIG. 6: Alignment of the full-length sequences of rBoNT/F.sub.1 (UPI00000B66D1), rBoNTF.sub.7 (UPI0001DE3DAC) and rBoNTF.sub.7-1. The light chain is shown in bold, the activation loop is double-underlined, the belt sequence is dotted underlined, the H.sub.N domain is italicised, the H.sub.CN domain is single underlined and the H.sub.CC domain is dashed underlined.

(7) FIG. 7: SDS PAGE Gel of purified chimeric rBoNTF.sub.7-1 SXN102875 Batch DB131202 (lane 1: molecular weight markers; lane 2: oxidised rBoNTF.sub.7-1 sample; lane 3: reduced rBoNTF.sub.7-1 sample), 1 g protein loaded per lane

(8) FIG. 8: N-terminal processing of rBoNTF.sub.7-1. FIG. 8A is a schematic identifying the cleavage site within the activation loop. FIG. 8B is an SDS-PAGE gel confirming the presence of the activated di-chain rBoNTF.sub.7-1. FIG. 8C shows the results of the N-terminal sequencing. FIG. 8D is an alignment of a section comprising the C-terminal end of the light chain, the activation loop and the N-terminal end of the H.sub.N domain of the different BoNT/F subtypes. The cysteine residues forming the neck of the activation loop are bold and underlined. The BoNTF.sub.1 residues found at the N-terminus of the rBoNTF.sub.7-1 H.sub.N domain are bold, italicised, and shaded.

(9) FIG. 9: Cell-free protease activity of purified Chimeric rBoNT/F7-1. A recombinant substrate protein VAMP-2.sub.2-94-GFP was incubated with either recombinant (rBoNT/F.sub.7-1) or native (nBoNT/F) BoNT/F, at 37 C., for 30 min, before the reaction was stopped and analysed by reverse phase chromatography. FIG. 9A is a diagram showing the cleavage reaction in which the substrate (VAMP-2.sub.2-94-GFP) is converted to products (VAMP2.sub.2-58 and VAMP-2.sub.59-94-GFP). Panel FIG. 9B shows an example chromatogram to quantify the uncleaved substrate and the cleaved VAMP-2.sub.59-94-GFP product present in the stopped reaction mix. FIG. 9C shows an example of the proportion of substrate converted to product and the calculated specific activity (nmol substrate converted to product, per hour, per pmol BoNT) for recombinant and native BoNT/F proteins.

(10) FIG. 10: Inhibition of SCN Gly release by chimeric rBoNT/F.sub.7-1. Cultured primary spinal cord neurons (SCN) were exposed to various concentrations of recombinant (rBoNT/F.sub.7-1) or native (Met/F/080116/MET) BoNT/F for 24 hours, then loaded with radiolabelled glycine ([.sup.3H]-Glycine), and depolarised (with high potassium buffer) to stimulate neurotransmitter (glycine) release.

(11) FIG. 11: Inhibition of ex-vivo muscle contraction by chimeric rBoNT/F.sub.7-1. An isolated phrenic nerve/hemi-diaphragm tissue preparation was set up in a tissue bath. The hemi-diaphragm muscle was attached to a force transducer (to measure contraction) and the pherenic nerve was electro-stimulated, at 1 Hz, (to stimulate muscle contraction). Contractile force was measured continuously for a period up to 100 min after addition of 100 pM BoNT/F. The decrease in force (compared to control) was plotted against time and the time needed, from addition of BoNT, until 50% reduction in the contractile force was recorded as the t50.

(12) FIG. 12: mouse Digit Abduction Scoring assay. Mice received injection, into the gastrocnemius-soleus complex muscles of one hind limb, under short general anesthesia; muscle weakening was measured on a 0-4 scale using the digit abduction score (DAS). DAS max values were determined for each dose and plotted against dose and the data were fitted to a 4-parameter logistic equation, ED50 and dose leading to DAS 4 (DAS 4 dose) values were determined (see table).

DETAILED DESCRIPTION OF THE INVENTION

(13) Botulinum Toxins (BoNTs)

(14) Botulinum neurotoxin (BoNT) is produced by C. botulinum in the form of a large protein complex, consisting of BoNT itself complexed to a number of accessory proteins. There are at present eight different classes of botulinum neurotoxin, namely: botulinum neurotoxin serotypes A, B, C.sub.1, D, E, F, G, and H, all of which share similar structures and modes of action. Different BoNT serotypes can be distinguished based on inactivation by specific neutralising anti-sera, with such classification by serotype correlating with percentage sequence identity at the amino acid level. BoNT proteins of a given serotype are further divided into different subtypes on the basis of amino acid percentage sequence identity.

(15) BoNTs are absorbed in the gastrointestinal tract, and, after entering the general circulation, bind to the presynaptic membrane of cholinergic nerve terminals and prevent the release of their neurotransmitter acetylcholine. BoNT/B, BoNT/D, BoNT/F and BoNT/G cleave synaptobrevin/vesicle-associated membrane protein (VAMP); BoNT/C.sub.1, BoNT/A and BoNT/E cleave the synaptosomal-associated protein of 25 kDa (SNAP-25); and BoNT/C.sub.1 cleaves syntaxin.

(16) TeNT is produced in a single serotype by C. tetani. C. butyricum produces BoNT/E, while C. baratii produces BoNT/F.

(17) The present invention provides polypeptides comprising chimeric BoNT LH.sub.N domains, and so also encompasses chimeric clostridial toxins. The term clostridial toxin is also intended to embrace modified clostridial toxins and derivatives thereof, including but not limited to those described below. A modified clostridial toxin or derivative may contain one or more amino acids that has been modified as compared to the native (unmodified) form of the clostridial toxin, or may contain one or more inserted amino acids that are not present in the native (unmodified) form of the clostridial toxin. By way of example, a modified clostridial toxin may have modified amino acid sequences in one or more domains relative to the native (unmodified) clostridial toxin sequence. Such modifications may modify functional aspects of the toxin, for example biological activity or persistence. Thus, in one embodiment, the engineered clostridial toxin of the invention is an engineered modified clostridial toxin, or an engineered modified clostridial toxin derivative, or an engineered clostridial toxin derivative.

(18) A reference herein to a particular BoNT serotype typically refers to all subtypes within that serotype unless explicitly stated to the contrary.

(19) The present inventors have analysed the exposed loop region within the BoNT light and heavy chains, also known as the activation loop or the di-chain loop, for all subtypes of BoNT serotypes for which amino acid sequences are known. In more detail, the inventors have aligned and annotated the activation loop sequences of all full-length BoNT proteins identified in the UniParc database (which removes redundant sequences). The annotated alignments from all BoNT/A and BoNT/F subtypes are shown in FIG. 1. The inventors have surprisingly found that the BoNT/F subtypes have the most divergent activation loops of all subtypes aligned.

(20) Naturally occurring BoNT proteins are produced within C. botulinum bacteria as single chain, precursor proteins. The single chain forms become post-translationally modified, by cleavage (also referred to herein as proteolysis) within the activation loop, to produce fully active, mature, di-chain BoNT proteins. The inventors have previously produced recombinant BoNT proteins, as single chain precursor proteins, in Escherichia coli then performed the post-translational proteolytic step on the purified single chain BoNT. Unfortunately, not all purified recombinant BoNT proteins show good activation, where good activation is defined as specific proteolysis at the same site as the naturally occurring protein and not at other sites within the recombinant BoNT. For example, a naturally occurring BoNT protein, subtype BoNT/F.sub.7, becomes proteolysed at multiple sites, by all proteases so far tested. The inventors have solved the problem (of activating subtype BoNT/F.sub.7) by constructing a chimeric protein in which the region containing the site of desired proteolytic cleavage (called the activation loop) was removed from subtype F.sub.7 and replaced it with the equivalent region from another, highly related, BoNT (called subtype BoNT/F.sub.1). The inventors have previously identified conditions under which subtype BoNT/F.sub.1 does become site-specifically activated. Thus, by substituting the activation loop of BoNT/F.sub.7 with the activation loop of BoNT/F.sub.1, the inventors have demonstrated that it is possible to generate recombinant BoNT with good activation properties by substituting the activation loop of one BoNT subtype with the activation loop from another BoNT subtype from the same serotype. Without being bound by theory, it is believed that this intra-serotype activation loop swapping is particularly relevant to BoNT/F subtypes due to the divergence in sequence between the activation loops of the different BoNT/F subtypes. The present invention also provides (activated) chimeric di-chain polypeptides. These di-chain polypeptides are themselves chimeric, because they contain both an amino acid sequence from the BoNT/F subtype from which the LH.sub.N backbone is derived and an amino acid sequence from the BoNT/F subtype from which the activation loop is derived. Thus, the resulting chimeric di-chain has a unique amino acid sequence found neither in the BoNT/F subtype from which the LH.sub.N backbone is derived nor in the BoNT/F subtype from which the activation loop is derived. The chimeric di-chain polypeptides of the invention are typically produced by cleavage of the chimeric single chain polypeptides of the invention.

(21) Thus, the present invention provides a means of making fully active, purified, recombinant chimeric BoNTs, containing functional domains with desirable properties, even when those domains have evolved within proteins which are not easily expressed, purified and activated in recombinant form.

(22) Accordingly, the invention provides a chimeric single-chain polypeptide comprising an LH.sub.N backbone from a first botulinum neurotoxin serotype F (BoNT/F) subtype and an activation loop region from a different BoNT/F subtype.

(23) In the context of the present invention, the term chimeric polypeptide is one which comprises component parts from two different BoNT/F subtypes. In particular, the chimeric single-chain polypeptide of the invention comprises an LH.sub.N backbone from a first BoNT/F subtype and an activation loop region from a different BoNT/F subtype. A chimeric di-chain polypeptide contains an amino acid sequence from the LH.sub.N backbone of a first BoNT/F subtype and an amino acid sequence from the activation loop of a second BoNT/F subtype, as described herein.

(24) Naturally occurring BoNT/F serotype proteins are classified into seven subtypes (FIG. 2). The light chain of BoNT/F provides a protease function (also known as a non-cytotoxic protease). In particular, the BoNT/F light chain demonstrates a metalloprotease activity and is capable of proteolytically cleaving a vesicle and/or plasma membrane associated protein involved in cellular exocytosis. Typically the substrates for all BoNT/F subtype light chains are vesicle-associated membrane proteins 1, 2 and 3 (VAMP1, VAMP2, and VAMP3). The protease activity of a BoNT light chain or a BoNT/F LH.sub.N backbone may be determined by any appropriate techniques. Standard techniques for determining the cleavage activity of the light chain or fragment or variant thereof are known in the art, for example a mouse hemidiaphragm assay (HDA), or SNARE cleavage assays (see, for example, WO95/33850 and Masuyer G, Beard M, Cadd V A, Chaddock J A, Acharya K R; Structure and activity of a functional derivative of Clostridium botulinum neurotoxin B; J Struct Biol. 2011 April; 174(1):52-7), both of which are herein incorporated by reference).

(25) Designation of different BoNTs into serotypes is determined by serological means, rather than being defined by amino acid sequence. Indeed, classification of BoNTs into serotypes A, B, C, D, E, and F, is based solely on, and defined by, their sensitivity to neutralisation by a set of monovalent antitoxins, distributed by the US government Centers for Disease Control and Prevention (CDC), in a standardised mouse bioassay [Botulism in the United States, 1899-1996, Handbook for epidemiologists, clinicians, and laboratory workers; Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Bacterial and Mycotic Diseases, 1998, herein incorporated by reference in its entirety]. In this regard, see also: Dezfulian and Bartlett J. Clin. Microbiol. 21(2): 231-233, or Lindstrom and Korkeala Clin. Microbiol. Rev. 19(2): 298-314, 2006, which disclose that serological means are used to define and distinguish between BoNT serotypes, both of which are incorporated by reference.

(26) Accordingly, a reference herein to BoNT/F is a reference to a BoNT that is neutralised by a reference anti-F-serotype monovalent antitoxin as described above. Thus, typically the LH.sub.N backbone of the present invention comprises: (a) a light chain polypeptide sequence which is from a BoNT neutralised by a reference anti-F-serotype monovalent antitoxin; and (b) a H.sub.N polypeptide sequence which is from a BoNT neutralised by a reference anti-F-serotype monovalent antitoxin. The chimeric di-chain polypeptide of the present invention may be neutralised by a reference anti-F-serotype monovalent antitoxin as described herein. In one embodiment of the invention, the term BoNT/F embraces BoNT/F hybrid molecules, such as the recently identified BoNT/H, which is believed to be a hybrid of BoNT/F and BoNT/A.

(27) The light chain polypeptide sequence and/or H.sub.N polypeptide sequence of the LH.sub.N backbone of the present invention and/or the di-chain polypeptide of the present invention may also be defined in terms of their sequence identity to known BoNT/F subtypes, as described herein.

(28) According to the present invention, a BoNT/F LH.sub.N backbone may be defined as the region of a BoNT/F neurotoxin which comprises the BoNT/F light chain and the H.sub.N domain. The term LH.sub.N backbone as used herein also embraces LH.sub.N backbones comprising functional fragments or variants of the BoNT/F light chain and/or functional fragments or variants of the BoNT/F H.sub.N domain. Functional fragments or variants of the BoNT/F H.sub.N domain retain the translocation activity of the full-length BoNT/F H.sub.N domain. Functional fragments or variants of the BoNT/F light chain retain the cleavage activity of the full-length light chain as defined herein. In other words, functional BoNT/F light chain fragments or variants can specifically target the core components of the neurotransmitter release apparatus and thus participate in executing the overall cellular mechanism whereby a BoNT/F toxin proteolytically cleaves a substrate. A variant of the BoNT/F light chain as comprised in the BoNT/F LH.sub.N backbone may have at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% sequence identity with the BoNT/F light chain, provided it retains the cleavage activity of the native (unmodified) light chain.

(29) Functional fragments of the BoNT/F light chain include the shortest possible BoNT/F light chain sequence which retains the activity of the full-length light chain, i.e. the minimal BoNT/F light chain fragment, as defined herein. Similarly, functional fragments of the BoNT/F H.sub.N domain include the shortest possible BoNT/F H.sub.N domain sequence which retains the activity of the full-length H.sub.N domain, i.e. the minimal BoNT/F H.sub.N domain fragment, as defined herein.

(30) A BoNT/F LH.sub.N backbone of the invention may have one or more modifications in the amino acid sequence of the BoNT/F light chain sequence, for example modifications in the substrate binding or catalytic domain which may alter or modify the substrate specificity of the modified light chain, and/or which alter or modify the activity of the modified light chain, such as modifying the potency and/or duration of the modified light chain activity. Such modified light chains may have higher or lower activity (potency) and/or longer or shorter duration than a reference light chain. Said reference light chain is typically a native (unmodified) version of the corresponding light chain.

(31) For example, amino acid differences within the light chain sequence of one of the naturally occurring BoNT/F subtypes (subtype F.sub.5), compared to other BoNT/F sequences, underlie a difference in which peptide bond of VAMP2 BoNT/F.sub.5 cleaves (L54-E55 compared to other BoNT/F subtypes (which cleave between Q58-K59) [Kalb S R, Baudys J, Webb R P, Wright P, Smith T J, Smith L A, Fernndez R, Raphael B H, Maslanka S E, Pirkle J L, Barr J R.; FEBS Lett. 2012 Jan. 20; 586(2):109-15; Discovery of a novel enzymatic cleavage site for botulinum neurotoxin F.sub.5, which is herein incorporated by reference]. As another example, the light chain sequence may be PEGylated, which increases the duration of the modified light chain activity (see, for example, WO2007/104567, which is herein incorporated by reference) The light chain sequence may contain a degradation sequence, or be ubiquitinated, which decreases the duration of activity of the modified light chain (see, for example, WO 2011/000929, WO 2013/017522, WO 2013/068476, WO 2014/029497 and R. V. Kukreja, et al., Biochim Biophys Acta. (2007) 1774(2):213-22, each of which is incorporated by reference). The light chain sequence may be modified to increase potency, for example by optimisation of the substrate recognition pockets (see, for example, Guo et al. 2013, Toxicon 74: 158-166, which is herein incorporated by reference).

(32) The light chains of clostridial toxins are approximately 420-460 amino acids in length and comprise an enzymatic domain. Typically a full-length BoNT/F light chain is 430 amino acids in length (such as amino acid positions corresponding to residues 1 to 430 of any one of SEQ ID NOs: 1 to 16, including the full-length BoNT/F consensus sequence SEQ ID NO: 1). Thus, a reference herein to a BoNT/F light chain may relate to an amino acid sequence comprising or consisting of amino acids corresponding to residues 1 to 430 of SEQ ID NO: 1, such as amino acid residues from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 1 to 430 of SEQ ID NO: 1 (as shown in FIG. 4 or Table 1), or a variant thereof.

(33) Research has shown that the entire length of a clostridial toxin light chain is not necessary for the enzymatic activity of the enzymatic domain. Thus, a fragment of the BoNT/F light chain as comprised in LH.sub.N backbone may comprise at least 100, at least 200, at least 250, at least 300, at least 350, at least 400, at least 450, or more continuous or discontinuous amino acids from the BoNT/F light chain, provided it retains the cleavage activity of the native (unmodified) light chain. Typically the fragment of the BoNT/F light chain comprises at least 350, at least 400, at least 450 or more continuous amino acids from the BoNT/F light chain. The light chain portion of the LH.sub.N backbone of the invention may comprise or consist of a minimal BoNT/F light chain fragment, which itself may comprise or consist of amino acid residues corresponding to residues 15 to 412 of any one of SEQ ID NOs: 1 to 16, or amino acids positions from any one of SEQ ID NOs: 2 to 16, preferably SEQ ID NO: 13, which correspond to amino acid residues 15 to 412 of the consensus sequence SEQ ID NO: 1 (for example, see Table 1), or a variant thereof. Suitable light chain fragments according to the invention are shown in bold in FIGS. 4 and 6 herein. A light chain fragment according to the invention may comprise or consist of an amino acid sequence of any one of SEQ ID NOs: 33 to 48, preferably SEQ ID NO: 45 (which define the minimal light chain fragments for non-redundant BoNT/F sequences), or a variant thereof.

(34) A variant of the BoNT/F light chain may have at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with the reference BoNT/F light chain, provided it retains the cleavage activity of the reference BoNT/F light chain. Typically the reference light chain domain or fragment is a native (unmodified) BoNT/F light chain.

(35) The term fragment also relates to the BoNT/F light chain variants described herein. Thus, by way of example, a BoNT/F light chain fragment of the invention may have at least 350, at least 400, at least 450 or more continuous amino acids, wherein the BoNT/F light chain amino acid sequence has at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity over a corresponding peptide sequence from the reference BoNT/F light chain. Thus, a variant BoNT/F light chain fragment may have at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity to any BoNT/F light chain fragment disclosed herein.

(36) In a preferred embodiment, the BoNT/F light chain or light chain fragment, or variant thereof, is from BoNT/F.sub.7 or BoNT/F.sub.5. Thus, a BoNT/F light chain of the invention may comprise or consist of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16 which corresponds to amino acids 1 to 430 of SEQ ID NO: 1. For example, a BoNT/F light chain of the invention may comprise or consist of amino acid residues 1 to 421 of any one of SEQ ID NOs: 13 to 16, preferably SEQ ID NO: 13, or amino acid residues 1 to 428 of SEQ ID NO: 8, or a fragment or variant thereof.

(37) Preferably the LH.sub.N backbone of the invention comprises a light chain fragment which comprises or consists of a minimal light chain fragment from BoNT/F.sub.7 or BoNT/F.sub.5. Thus, a BoNT/F light chain of the invention may comprise or consist of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably SEQ ID NO: 13, which corresponds to amino acids 15 to 412 of SEQ ID NO: 1. For example, a BoNT/F light chain of the invention may comprise or consist of amino acid residues 15 to 403 of any one of SEQ ID NOs: 13 to 16 (preferably from SEQ ID NO: 13), or amino acid residues 15 to 410 of SEQ ID NO: 8, or a fragment or variant thereof. The minimal light chain fragment from BoNT/F.sub.7 or BoNT/F.sub.5 may comprise or consist of the amino acid sequence of any one of SEQ ID NOs: 40 or 45 to 48, preferably SEQ ID NO: 45, or a variant thereof.

(38) The LH.sub.N backbone of the invention typically comprises a (BoNT/F) light chain polypeptide sequence having at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with at least one of SEQ ID NOs: 33 to 48, preferably at least one of SEQ ID NOs: 40 or 45 to 48, more preferably with SEQ ID NO: 45.

(39) There is evidence that BoNT/F subtypes 1, 2, 6 and 7 all cleave VAMP2 at the same peptide bond, namely between Q58-K59 in the human protein (see Kalb et al. (2011) App. Enviro. Microbiol. 77(4):1301-8, which is herein incorporated by reference) but that BoNT/F.sub.5 cleaves a different peptide bond, between L54-E55 (see Kalb et al. (2012) FEBS Lett. 586(2): 109-115, which is herein incorporated by reference).

(40) The LH.sub.N backbone of the invention may have at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of amino acid residues corresponding to residues 1 to 873 of any one of SEQ ID NOs: 1 to 16, or amino acids positions from any one of SEQ ID NOs: 2 to 16, preferably SEQ ID NO: 13, which correspond to amino acid residues 1 to 873 of the consensus sequence of SEQ ID NO: 1 (for example, see Table 1), or a variant thereof, wherein the activation loop sequence within the sequence of any one of SEQ ID NOs: 1 to 16 is replaced by the activation loop sequence from a different BoNT/F subtype (exemplary activation loop sequences are disclosed herein).

(41) The LH.sub.N backbone of the invention may be a minimal LH.sub.N backbone, comprising minimal light chain and H.sub.N domain fragments as disclosed herein. Thus, a minimal LH.sub.N backbone of the invention may have at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of amino acid residues corresponding to residues 15 to 850 of any one of SEQ ID NOs: 1 to 16, or amino acids positions from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 15 to 850 of the consensus sequence of SEQ ID NO: 1 (as shown in FIG. 4), or a variant thereof, wherein the activation loop sequence within the sequence of any one of SEQ ID NOs: 1 to 16 is replaced by the activation loop sequence from a different BoNT/F subtype.

(42) For example, the LH.sub.N backbone of the invention may have at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity or more sequence identity with any one of SEQ ID NOs: 17 to 32 (where SEQ ID NO: 17 is the BoNT/F LH.sub.N domain consensus sequence), preferably SEQ ID NO: 29.

(43) Preferably the LH.sub.N backbone of the invention comprises a light chain fragment and an H.sub.N domain from BoNT/F.sub.7 or BoNT/F.sub.5. Thus, a LH.sub.N backbone of the invention may comprise or consist of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13, which corresponds to amino acids 1 to 873, more preferably amino acids 15 to 850, of SEQ ID NO: 1. For example, a BoNT/F LH.sub.N backbone of the invention may comprise or consist of amino acid residues 1 to 862, more preferably amino acid residues 15 to 839, of any one of SEQ ID NOs: 13 to 16 (preferably SEQ ID NO: 13), or amino acid residues 1 to 868, more preferably 15 to 845 of SEQ ID NO: 8, or a fragment or variant thereof, provided the activation loop sequence within the sequence of any one of SEQ ID NOs: 8 or 13 to 16 (preferably SEQ ID NO: 13) is replaced by the activation loop sequence from a different BoNT/F subtype as disclosed herein.

(44) In a preferred embodiment, the LH.sub.N backbone of the invention has at least 60% or more sequence identity with at least one of SEQ ID NOs: 24 or 29 to 32, preferably SEQ ID NO: 29, provided the activation loop sequence within the sequence of any one of SEQ ID NOs: 1 to 16 is replaced by the activation loop sequence from a different BoNT/F subtype.

(45) Typically, a variant LH.sub.N backbone of the invention has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with of the LH.sub.N backbone sequences defined herein. As a non-limiting example, a variant LH.sub.N backbone of the invention may have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with at least one of SEQ ID NOs: 24 or 29 to 32, preferably SEQ ID NO: 29.

(46) The LH.sub.N backbone of the invention typically comprises a BoNT/F light chain fragment and an H.sub.N domain as disclosed herein, preferably a minimal BoNT/F light chain polypeptide sequence and a minimal H.sub.N domain fragment as disclosed herein.

(47) The H.sub.N domain of clostridial toxins are approximately 300-400 amino acids in length and comprise a translocation domain. Typically a full-length BoNT/F H.sub.N domain is approximately 350 amino acids in length (for example, amino acid positions corresponding to residues 520 to 873 of any one of SEQ ID NOs: 1 to 16, including the full-length BoNT/F consensus sequence SEQ ID NO: 1). Thus, a reference herein to a BoNT/F H.sub.N domain may relate to an amino acid sequence comprising or consisting of amino acids corresponding to residues 520 to 873 of SEQ ID NO: 1, such as amino acid residues from any one of SEQ ID NOs: 2 to 16 which correspond to amino acid residues 520 to 873 of SEQ ID NO: 1 (as shown in FIG. 4 and Table 1), or a variant thereof.

(48) According to the present invention, the BoNT/F LH.sub.N backbone comprises a BoNT/F H.sub.N domain. The term LH.sub.N backbone as used herein also embraces LH.sub.N backbones comprising functional fragments or variants of the BoNT/F H.sub.N domain. Functional fragments or variants of the BoNT/F H.sub.N domain retain the translocation activity of the full-length H.sub.N domain as defined herein. In other words, functional BoNT/F H.sub.N domain fragments or variants are capable of forming ion-permeable pores in lipid membranes (e.g. endosomal membranes) under conditions of low pH, enabling the BoNT/F light chain to escape the endosome.

(49) Thus, a fragment of the BoNT/F H.sub.N domain as comprised in LH.sub.N backbone may comprise at least 100, at least 200, at least 250, at least 300, at least 350, at least 400 or more continuous or discontinuous amino acids from the BoNT/F H.sub.N domain, provided it retains the translocation activity of a reference H.sub.N domain. The reference H.sub.N domain may be a native (unmodified) H.sub.N domain. Typically the fragment of the BoNT/F H.sub.N domain comprises at least 300, at least 350, at least 400 or more continuous amino acids from the BoNT/F H.sub.N domain.

(50) The H.sub.N domain portion of the LH.sub.N backbone of the invention may comprise or consist of a minimal BoNT/F H.sub.N domain fragment, which itself may comprise or consist of amino acid residues corresponding to residues 520 to 873 of any one of SEQ ID NOs: 1 to 16, or amino acids positions from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13 which correspond to amino acid residues 520 to 873 of the consensus sequence SEQ ID NO: 1 (for example, see Table 1), or a variant thereof. Suitable H.sub.N domain fragments according to the invention are italicised in FIGS. 4 and 6 herein.

(51) An H.sub.N domain fragment according to the invention may comprise or consist of an amino acid sequence of any one of SEQ ID NOs: 49 to 64, preferably SEQ ID NO: 61 (which define the minimal H.sub.N domain fragments for non-redundant BoNT/F sequences), or a variant thereof. A variant of the BoNT/F H.sub.N domain may have at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with the reference BoNT/F H.sub.N domain, provided it retains the translocation activity of the reference BoNT/F H.sub.N domain. Typically the reference H.sub.N domain domain or fragment is a native (unmodified) BoNT/F H.sub.N domain as disclosed herein.

(52) The term fragment also relates to the BoNT/F H.sub.N domain variants described herein. Thus, by way of example, a BoNT/F H.sub.N domain fragment of the invention may have at least 300, at least 350, at least 400 or more continuous amino acids, wherein the BoNT/F H.sub.N domain amino acid sequence has at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity over a corresponding peptide sequence from the reference BoNT/F H.sub.N domain. Thus, a variant BoNT/F H.sub.N domain fragment may have at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity to any BoNT/F H.sub.N domain fragment disclosed herein.

(53) In a preferred embodiment, a variant of the BoNT/F H.sub.N domain, or fragment or variant thereof may have at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with the reference BoNT/F H.sub.N domain, provided it retains the translocation activity of the reference BoNT/F H.sub.N domain. Typically the reference H.sub.N domain is a native (unmodified) BoNT/H.sub.N domain.

(54) In a preferred embodiment, the BoNT/F H.sub.N domain or H.sub.N domain fragment, or variant thereof, is from BoNT/F.sub.7 or BoNT/F.sub.5. Thus, a BoNT/F H.sub.N domain of the invention may comprise or consist of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13, which corresponds to amino acids 520 to 873 of SEQ ID NO: 1 (for example, see Table 1). For example, a BoNT/F H.sub.N domain of the invention may comprise or consist of amino acid residues 509 to 862 of any one of SEQ ID NOs: 13 to 16, preferably from SEQ ID NO: 13, or amino acid residues 516 to 868 of SEQ ID NO: 8, or a fragment or variant thereof.

(55) Preferably the LH.sub.N backbone of the invention comprises a H.sub.N domain fragment which comprises or consists of a minimal H.sub.N domain fragment from BoNT/F.sub.7 or BoNT/F.sub.5. Thus, a BoNT/F H.sub.N domain of the invention may comprise or consist of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13, which corresponds to amino acids 538 to 850 of SEQ ID NO: 1 (for example, see Table 1). For example, a BoNT/F H.sub.N domain of the invention may comprise or consist of amino acid residues 527 to 839 of any one of SEQ ID NOs: 13 to 16, preferably SEQ ID NO: 13, or amino acid residues 534 to 845 of SEQ ID NO: 8, or a fragment or variant thereof. The minimal H.sub.N domain fragment from BoNT/F.sub.7 or BoNT/F.sub.5 may comprise or consist of the amino acid sequence of any one of SEQ ID NOs: 56 or 61 to 64, preferably SEQ ID NO: 61, or a variant thereof.

(56) The LH.sub.N backbone of the invention typically comprises a (BoNT/F) H.sub.N domain polypeptide sequence having at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with at least one of SEQ ID NOs: 49 to 64, preferably at least one of SEQ ID NOs: 56 or 61 to 64, more preferably SEQ ID NO: 61. In some embodiments, the H.sub.N domain of the invention (or fragment or variant thereof) may additionally comprise one or more amino acids from a belt region polypeptide sequence. For example, the H.sub.N domain of the invention (or fragment or variant thereof) may additionally comprise a full-length belt region polypeptide sequence or a fragment or variant thereof. A BoNT belt region is the polypeptide sequence that is located between the activation loop and the start of the H.sub.N domain; it is so named because that region of the heavy chain sequence wraps around the BoNT light chain sub-unit, in the three dimensional structure of the protein, in a way that is reminiscent of a belt wrapping around a person's waist. The position of the belt region in known BoNT/F sequences is shown in Table 1 below (the recited amino acid positions correspond to the positions in the full length BoNT sequence). Thus, a reference herein to a H.sub.N domain of the invention may refer to a H.sub.N domain polypeptide sequence including or excluding one or more amino acids from a belt region polypeptide.

(57) In some embodiments, the present invention relates to a BoNT/F polypeptide sequence which comprises a light chain (or fragment or variant thereof as defined herein), a H.sub.N domain (or fragment or variant thereof as defined herein) and a belt region polypeptide (or fragment or variant thereof) from one BoNT/F subtype, preferably BoNT/F.sub.7 or BoNT/F.sub.5, and an activation loop from a different BoNT/F subtype, such as BoNT/F.sub.1, BoNT/F.sub.2, BoNT/F.sub.3, BoNT/F.sub.4, or BoNT/F.sub.6. The BoNT/F light chain fragment and/or H.sub.N domain fragment may be minimal fragments as disclosed herein, or variants thereof. Alternatively, chimeric BoNT/F of the invention may be derived from or essentially identical to a native full-length BoNT/F polypeptide (including a light chain, H.sub.N domain and belt region) from one BoNT/F subtype, preferably BoNT/F.sub.7 or BoNT/F.sub.5, except that the activation loop from the native BoNT/F has been replaced with the activation loop from a different BoNT/F subtype, such as BoNT/F.sub.1, BoNT/F.sub.2, BoNT/F.sub.3, BoNT/F.sub.4, or BoNT/F.sub.6.

(58) Typically, the LH.sub.N backbone of the invention comprises: (a) a (BoNT/F) polypeptide sequence having at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of amino acids corresponding to residues 1 to 430 of SEQ ID NO: 1 (such as amino acid residues from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 1 to 430 of SEQ ID NO: 1); and/or amino acid residues corresponding to residues 15 to 412 of any one of SEQ ID NOs: 1 to 16, or an amino acid sequence comprising or consisting of amino acids positions from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 15 to 412 of SEQ ID NO: 1; and/or an amino acid sequence which comprises or consists of any one of SEQ ID NOs: 33 to 48, preferably SEQ ID NO: 45; and (b) a (BoNT/F) polypeptide sequence having at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of amino acids corresponding to residues 520 to 873 of any one of SEQ ID NOs: 1 (such as amino acid residues from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 520 to 873 of SEQ ID NO: 1); and/or an amino acid sequence comprising or consisting of amino acid positions from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 520 to 873 of SEQ ID NO: 1; and/or an amino acid sequence which comprises or consists of any one of SEQ ID NOs: 49 to 64, preferably SEQ ID NO: 61.

(59) In a preferred embodiment, the LH.sub.N backbone of the invention comprises a BoNT/F.sub.7 or BoNT/F.sub.5 light chain or light chain fragment and a BoNT/F.sub.7 or BoNT/.sub.5 H.sub.N domain or H.sub.N domain fragment. Thus, in a preferred embodiment, the LH.sub.N backbone of the invention comprises: (a) a (BoNT/F) polypeptide sequence having at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13 which corresponds to amino acids 1 to 430 of SEQ ID NO: 1, such as amino acid residues 1 to 421 of any one of SEQ ID NOs: 13 to 16, preferably from SEQ ID NO: 13, or amino acid residues 1 to 428 of SEQ ID NO: 8; and/or an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13, which corresponds to amino acids 15 to 412 of SEQ ID NO: 1, such as amino acid residues 15 to 403 of any one of SEQ ID NOs: 13 to 16, preferably from SEQ ID NO: 13, or amino acid residues 15 to 410 of SEQ ID NO: 8; and/or any one of SEQ ID NOs: 40 or 45 to 48, preferably SEQ ID NO: 45; and (b) at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16 which corresponds to amino acids 520 to 873 of SEQ ID NO: 1, such as amino acid residues 509 to 862 of any one of SEQ ID NOs: 13 to 16, preferably from SEQ ID NO: 13, or amino acid residues 516 to 868 of SEQ ID NO: 8; and/or an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably SEQ ID NO: 13 which corresponds to amino acids 538 to 850 of SEQ ID NO: 1, such as amino acid residues 527 to 839 of any one of SEQ ID NOs: 13 to 16, preferably SEQ ID NO: 13, or amino acid residues 534 to 845 of SEQ ID NO: 8, and/or any one of SEQ ID NOs: 56 or 61 to 64, preferably SEQ ID NO: 61.

(60) The polypeptide of the invention comprises a site for cleavage by a proteolytic enzyme, which cleavage site is an activation loop from a different botulinum type F (BoNT/F) subtype than the BoNT/F LH.sub.N backbone. The BoNT/F activation loop enables the cleavage of the single-chain polypeptide into a di-chain form. Cleavage into the di-chain form typically activates the polypeptide, which is necessary to free the light chain or fragment or variant thereof to carry out its proteolytic cleavage activity in the cell cytoplasm.

(61) As used herein, the term BoNT/F activation loop embraces fragments and variants thereof, provided said fragments or variants retain the ability to be cleaved, producing the di-chain form of the polypeptide, activating said polypeptide and freeing the light chain or fragment or variant thereof to carry out its proteolytic cleavage activity. The BoNT/F activation loop may be cleaved by any appropriate protease (Lys-C). The ability of any given protease to cleave at the activation loop may be determined by any appropriate technique known in the art, see for example, WO 1995/033850, Dekleva et al., Biochem. Biophys. Res. Commun. (1989) 162(2):767-72, Giminez, Biochimie. (1990) 72(4):213-7 and Giminez, J. Protein Chem. (1993) 12(3):351-63, each of which is herein incorporated by reference.

(62) The activation loop of the polypeptide of the invention typically comprises an amino acid sequence fitting the consensus sequence KSIIPRKGTKAPPRL (SEQ ID NO: 65), or an amino acid sequence in which no more than five, no more than four, no more than three, no more than two or no more than one amino acids are added to, deleted from, or substituted in the consensus sequence.

(63) The activation loop may comprise an amino acid sequence selected from (a) KSVIPRKGTKAPPRL (SEQ ID NO: 66); (b) KSIIPRKGTKQSPSL (SEQ ID NO: 67); or (c) KSIIPRKGTKAPPRL (SEQ ID NO: 65), or an amino acid sequence in which no more than five, no more than four, no more than three, no more than two or no more than one amino acids are added to, deleted from, or substituted in the sequence. Preferably the activation loop comprises the amino acid sequence KSVIPRKGTKAPPRL (SEQ ID NO: 66, or an amino acid sequence in which no more than five, no more than four, no more than three, no more than two or no more than one amino acids are added to, deleted from, or substituted in the sequence.

(64) Table 1 (below) shows the precursor, the native di-chain neurotoxin of BoNT/F subtypes and identifies the light chain, the exposed loop (activation loop), the belt region, the H.sub.N domain and the H.sub.C domain (including the H.sub.CN and H.sub.CC domains).

(65) The reference sequence should be considered a guide as slight variations may occur according to subtypes.

(66) Referring to Table 1, the amino acid positions (residues) indicated for the light chains, H.sub.N, H.sub.CN and H.sub.CC domains correspond to the amino acid positions for said regions in a full length BoNT/F protein sequence. The UniParc Accession Numbers given in Table 1 are examples of the different BoNT/F sub-serotypes. Thus, in one embodiment, the amino acid positions of the light chains, H.sub.N, H.sub.CN and H.sub.CC domains indicated in Table 1 correspond to the amino acid positions in the full length BoNT/F protein sequences identified by Accession Number. The Accession Numbers given in Table 1 above reference the corresponding amino acid sequences as entered in the UniParc database as of the [INSERT FILING DATE OF APPLICATION].

(67) TABLE-US-00001 TABLE 1 UniParc Database no. Heavy Chain of the protein Total Position of Light Position of H.sub.C BoNT sequence length Chain Activation Loop Belt region H.sub.N H.sub.CN H.sub.CC BoNT/F consensus sequence 1283 1-430 431-445 446-519 520-873 874-1090 1091-1282 BoNT/F.sub.1 UPI00000B66D1 1278 1-429 430-444 445-518 519-871 872-1085 1086-1278 BoNT/F.sub.2 UPI00016BB5D7 1280 1-429 430-444 445-518 519-871 872-1088 1089-1280 BoNT/F.sub.2 UPI00000B50F1 1280 1-429 430-444 445-518 519-871 872-1088 1089-1280 BoNT/F.sub.3 UPI0001C0B12F 1279 1-429 430-444 445-518 519-871 872-1088 1089-1279 BoNT/F.sub.4 UPI0001A92658 1278 1-429 430-444 445-518 519-871 872-1085 1086-1278 BoNT/F.sub.4 UPI0001C0B12D 1277 1-429 430-444 445-518 519-871 872-1085 1086-1277 BoNT/F.sub.5 UPI0001C0B12E 1277 1-428 429-441 442-515 516-868 869-1085 1086-1277 BoNT/F.sub.6 UPI0001C0B1D1 1275 1-429 430-444 445-518 519-871 872-1088 1089-1275 BoNT/F.sub.6 UPI0000126B8A 1274 1-429 430-444 445-518 519-870 871-1087 1088-1274 BoNT/F.sub.6 UPI0001C0B1D0 1275 1-429 430-444 445-518 519-871 872-1088 1089-1275 BoNT/F.sub.6 UPI0004E7C8CE 1275 1-429 430-444 445-518 519-871 872-1088 1089-1275 BoNT/F.sub.7 UPI0001DE3DAC 1268 1-421 422-434 435-508 509-862 863-1076 1077-1268 BoNT/F.sub.7 UPI0001C0B220 1268 1-421 422-434 435-508 509-862 863-1076 1077-1268 BoNT/F.sub.7 UPI00000BAF8C 1268 1-421 422-434 435-508 509-862 863-1076 1077-1268 BoNT/F.sub.7 UPI0001C0B221 1268 1-421 422-434 435-508 509-862 863-1076 1077-1268

(68) A polypeptide of the invention may comprise an LH.sub.N backbone comprising a sequence from a light chain of any one of the BoNT/F subtypes identified in Table 1, or a fragment (such as a minimal light chain fragment as disclosed herein) or variant thereof. The domain structure of BoNTs is well known in the art and so it is sufficient to refer to the light chain of any particular BoNT, or a fragment or variant thereof. Alternatively, the BoNT/F light chain or fragment or variant thereof may be defined in terms of its sequence or in terms of the amino acid positions corresponding to the amino acid positions in the full-length sequence any one of the BoNT/F subtypes in Table 1 (as described herein). For example, the LH.sub.N backbone may comprise the light chain of the BoNT/F.sub.7 of UPI0001DE3DAC. This light chain may further be defined in terms of its sequence (e.g. the minimal light chain fragment of UPI0001DE3DAC has the amino acid sequence of SEQ ID NO: 45), or in terms of the amino acid corresponding to the amino acid positions in the full-length amino acid sequence of the BoNT/F.sub.7 of UPI0001DE3DAC (e.g. amino acids corresponding to amino acid positions 1 to 421 (for the full-length light chain sequence) or amino acid positions 15 to 403 (for the minimal light chain sequence) of UPI0001DE3DAC (full length UPI0001DE3DAC has the amino acid sequence of SEQ ID NO: 13). Such definitions (in terms of sequence and/or amino acid position) apply equally to any other BoNT/F LH.sub.N backbones or light chains, including those listed in Table 1.

(69) An LH.sub.N backbone of the invention may comprise a H.sub.N domain of any one of the BoNT/F subtypes identified in Table 1, or a fragment (such as a minimal H.sub.N domain fragment as disclosed herein) or variant thereof. The domain structure of BoNTs is well known in the art and so it is sufficient to refer to the H.sub.N domain of any particular BoNT, or a fragment or variant thereof. Alternatively, the BoNT/F H.sub.N domain or fragment or variant thereof may be defined in terms of its sequence or in terms of the amino acid positions corresponding to the amino acid positions in the full-length sequence any one of the BoNT/F subtypes in Table 1 (as described herein). For example, the LH.sub.N backbone may comprise the H.sub.N domain of the BoNT/F.sub.7 of UPI0001DE3DAC. This H.sub.N domain may further be defined in terms of its sequence (e.g. the minimal H.sub.N domain fragment of UPI0001DE3DAC has the amino acid sequence of SEQ ID NO: 61), or in terms of the amino acid corresponding to the amino acid positions in the full-length amino acid sequence of the BoNT/F.sub.7 of UPI0001DE3DAC (e.g. amino acids corresponding to amino acid positions 509 to 862 (for the full-length H.sub.N domain sequence) or amino acid positions 527 to 839 (for the minimal H.sub.N domain sequence) of UPI0001DE3DAC (full length UPI0001DE3DAC has the amino acid sequence of SEQ ID NO: 13). Such definitions (in terms of sequence and/or amino acid position) apply equally to any other BoNT/F LH.sub.N backbones or H.sub.N domains, including those listed in Table 1.

(70) A polypeptide of the invention may comprise an activation loop of any one of the BoNT/F subtypes identified in Table 1, or a fragment or variant thereof. The domain structure of BoNTs is well known in the art and so it is sufficient to refer to the activation loop of any particular BoNT, or a fragment or variant thereof. Alternatively, the BoNT/F activation loop or fragment or variant thereof may be defined in terms of its sequence or in terms of the amino acid positions corresponding to the amino acid positions in the full-length sequence any one of the BoNT/F subtypes in Table 1 (as described herein). For example, the polypeptide may comprise the activation loop of the BoNT/F.sub.1 of UPI00000B66D1. This activation loop may further be defined in terms of its sequence (SEQ ID NO: 66), or in terms of the amino acid corresponding to the amino acid positions in the full-length amino acid sequence of the BoNT/F.sub.1 of UPI00000B66D1 (amino acids corresponding to amino acid positions 430 to 444 of UPI00000B66D1). Such definitions (in terms of sequence and/or amino acid position) apply equally to any other BoNT/F activation loops, including those listed in Table 1.

(71) According to the present invention, the polypeptide comprises an activation loop that is from a different BoNT/F subtype to the BoNT/F LH.sub.N backbone.

(72) Thus, the polypeptide of the invention may comprise a BoNT/F LH.sub.N backbone from any one of BoNT/F.sub.1, BoNT/F.sub.2, BoNT/F.sub.3, BoNT/F.sub.4, BoNT/F.sub.5, BoNT/F.sub.6, or BoNT/F.sub.7. Typically the BoNT/F LH.sub.N backbone is from a BoNT/F.sub.5, or BoNT/F.sub.7 subtype. In a preferred embodiment, the BoNT/F LH.sub.N backbone is from a BoNT/F.sub.7 subtype.

(73) The polypeptide of the invention may comprise a BoNT/F activation loop from any one of BoNT/F.sub.1, BoNT/F.sub.2, BoNT/F.sub.3, BoNT/F.sub.4, BoNT/F.sub.5, BoNT/F.sub.6, or BoNT/F.sub.7, provided that the activation loop is from a different BoNT/F subtype than the BoNT/F LH.sub.N backbone. Typically the BoNT/F activation loop is from any one of BoNT/F.sub.1, BoNT/F.sub.2, BoNT/F.sub.3, BoNT/F.sub.4, or BoNT/F.sub.6. In a preferred embodiment, the BoNT/F activation loop is from BoNT/F.sub.1 or BoNT/F.sub.6.

(74) The polypeptide of the invention typically comprises a BoNT/F LH.sub.N backbone from a BoNT/F.sub.5 or BONT/F.sub.7 subtype and a BoNT/F activation loop from a BoNT/F.sub.1, BoNT/F.sub.2, BoNT/F.sub.3, BoNT/F.sub.4 or BoNT/F.sub.6 subtype. In a preferred embodiment the polypeptide of the invention typically comprises a BoNT/F LH.sub.N backbone from a BoNT/F.sub.5 or BONT/F.sub.7 subtype and a BoNT/F activation loop from a BoNT/F.sub.1 or BoNT/F.sub.6 subtype.

(75) Chimeric LH.sub.N backbones, in which the light chain (or fragment or variant thereof) and H.sub.N domain (or fragment or variant thereof) are derived from different BoNT/F subtypes are encompassed by the present invention. For example, a chimeric LH.sub.N backbone according to the present invention may comprise a light chain (or fragment or variant thereof) from a BONT/F.sub.7 subtype and an H.sub.N domain (or fragment or variant thereof) from a BoNT/F.sub.5 subtype or vice versa. When the polypeptide of the invention comprises such a chimeric LH.sub.N backbone, typically the activation loop is derived from a different BoNT/F subtype than either the light chain (or fragment or variant thereof) or the H.sub.N domain (or fragment or variant thereof). As described herein, preferably the BoNT/F activation loop is from a BoNT/F.sub.1 or BoNT/F.sub.6 subtype. Thus, a chimeric single-chain polypeptide of the present invention may comprise a light chain (or fragment or variant thereof) from a first BoNT/F subtype (for example BoNT/F.sub.7), an activation loop from a second BoNT/F subtype (for example BoNT/F.sub.1) and an H.sub.N domain (or fragment or variant thereof) from a third BoNT/F subtype (for example BoNT/F.sub.5).

(76) The polypeptides of the present invention, especially the light chain component thereof, may be PEGylatedthis may help to increase stability, for example duration of action of the protease component. PEGylation preferably includes the addition of PEG to the N-terminus of the protease component. By way of example, the N-terminus of a light chain may be extended with one or more amino acid (e.g. cysteine) residues, which may be the same or different. One or more of said amino acid residues may have its own PEG molecule attached (e.g. covalently attached) thereto. An example of this technology is described in WO2007/104567, herein incorporated by reference.

(77) A polypeptide of the invention may comprise one or more modifications that increases or decreases the biological activity and/or the biological persistence of the polypeptide. For example, a polypeptide may comprise a leucine- or tyrosine-based motif, wherein said motif increases or decreases the biological activity and/or the biological persistence of the polypeptide. Suitable leucine-based motifs include xDxxxLL, xExxxLL, xExxxIL, and xExxxLM (wherein x is any amino acid). Suitable tyrosine-based motifs include Y-x-x-Hy (wherein Hy is a hydrophobic amino acid). Examples of modified clostridial toxins comprising leucine- and tyrosine-based motifs are described in WO 2002/08268, which is hereby incorporated by reference in its entirety.

(78) A polypeptide of the invention may comprise one or more cationic modifications. Such polypeptides have properties of increased tissue retention at the site of administration, and which accordingly exhibit a reduction in diffusion away from the administration site. Examples of such cationic clostridial toxins are described in PCT/GB2014/052097, which is hereby incorporated by reference in its entirety.

(79) As described herein, the various elements of the polypeptide of the invention (LH.sub.N backbone, activation loop and/or targeting moiety) may be modified, in that each element may independently comprise at least one amino acid modification, selected from: an amino acid substitution, an amino acid insertion, and an amino acid deletion.

(80) In an amino acid substitution, an amino acid residue that forms part of the clostridial toxin amino acid sequence is replaced with a different amino acid residue. The replacement amino acid residue may be one of the 20 standard amino acids in the Table below.

(81) TABLE-US-00002 AMINO ACID SIDE CHAIN Aspartic acid Asp D Charged (acidic) Glutamic acid Glu E Charged (acidic) Arginine Arg R Charged (basic) Lysine Lys K Charged (basic) Histidine His H Uncharged (polar) Asparagine Asn N Uncharged (polar) Glutamine Gln Q Uncharged (polar) Serine Ser S Uncharged (polar) Threonine Thr T Uncharged (polar) Tyrosine Tyr Y Uncharged (polar) Methionine Met M Uncharged (polar) Tryptophan Trp W Uncharged (polar) Cysteine Cys C Uncharged (polar) Alanine Ala A Uncharged (hydrophobic) Glycine Gly G Uncharged (hydrophobic) Valine Val V Uncharged (hydrophobic) Leucine Leu L Uncharged (hydrophobic) Isoleucine Ile I Uncharged (hydrophobic) Proline Pro P Uncharged (hydrophobic) Phenylalanine Phe F Uncharged (hydrophobic)

(82) The following amino acids are considered charged amino acids: aspartic acid (negative), glutamic acid (negative), arginine (positive), and lysine (positive).

(83) The following amino acids are considered uncharged, polar (meaning they can participate in hydrogen bonding) amino acids: asparagine, glutamine, histidine, serine, threonine, tyrosine, cysteine, methionine, tryptophan.

(84) The following amino acids are considered uncharged, hydrophobic amino acids: alanine, valine, leucine, isoleucine, phenylalanine, proline, and glycine.

(85) Alternatively, the replacement amino acid in an amino acid substitution may be a non-standard amino acid (an amino acid that is not part of the standard set of 20 described above). By way of example, the replacement amino acid may be a basic non-standard amino acid, e.g. L-Ornithine, L-2-amino-3-guanidinopropionic acid, or D-isomers of Lysine, Arginine and Ornithine). Methods for introducing non-standard amino acids into proteins are known in the art, and include recombinant protein synthesis using E. coli auxotrophic expression hosts.

(86) In an amino acid insertion, an additional amino acid residue (one that is not normally present) is incorporated into the polypeptide amino acid sequence, thus increasing the total number of amino acid residues in said sequence. In an amino acid deletion, an amino acid residue is removed from the polypeptide amino acid sequence, thus reducing the total number of amino acid residues in said sequence.

(87) Methods for modifying proteins by substitution, insertion or deletion of amino acid residues are known in the art. By way of example, amino acid modifications may be introduced by modification of a DNA sequence encoding the polypeptide. This can be achieved using standard molecular cloning techniques, for example by site-directed mutagenesis where short strands of DNA (oligonucleotides) coding for the desired amino acid(s) are used to replace the original coding sequence using a polymerase enzyme, or by inserting/deleting parts of the gene with various enzymes (e.g., ligases and restriction endonucleases). Alternatively a modified gene sequence can be chemically synthesised.

(88) The at least one amino acid modification may be selected from: substitution of an acidic amino acid residue with a basic amino acid residue; substitution of an acidic amino acid residue with an uncharged amino acid residue; substitution of an uncharged amino acid residue with a basic amino acid residue; insertion of a basic amino acid residue; and deletion of an acidic amino acid residue.

(89) In a preferred embodiment, the at least one amino acid modification is a substitution, which advantageously maintains the same number of amino acid residues in the polypeptide.

(90) A polypeptide of the invention may comprise more than one amino acid modification. Thus, in one embodiment, the polypeptide (as described above) comprises between 1 and 80 amino acid modifications (for example, between 1 and 70, between 1 and 60, between 1 and 50, between 1 and 40, between 1 and 30, between 1 and 20, between 1 and 10, or 9, 8, 7, 6, 5, 4, 3 or 2 amino acid modifications.

(91) Preferred target amino acids for modification may possess certain qualities. By way of example, a preferred target amino acid may be: (i) a surface exposed amino acid; (ii) located outside of the polypeptide secondary structure; (iii) located in region of the polypeptide that is non-essential for protein function; (iv) an amino acid whose identity is not conserved between clostridial toxin types, subtypes, or serotypes; (iv) an amino acid whose modification does not create a predicted ubiquitination site; or (v) any combination of the foregoing.

(92) The polypeptides of the present invention may be free from the complexing proteins that are present in a naturally occurring clostridial toxin complex.

(93) A polypeptide of the present invention may also comprise a limited number of non-standard amino acids. Thus, in addition to the 20 standard amino acids, non-standard amino acids (such as 4-hydroxyproline, 6-N-methyl lysine, 2-aminoisobutyric acid, isovaline and -methyl serine) may be substituted for amino acid residues of the polypeptides of the present invention. A limited number of non-conservative amino acids, amino acids that are not encoded by the genetic code, and unnatural amino acids may be substituted for clostridial polypeptide amino acid residues. The polypeptides of the present invention can also comprise non-naturally occurring amino acid residues. Non-naturally occurring amino acids include, without limitation, trans-3-methylproline, 2,4-methano-proline, cis-4-hydroxyproline, trans-4-hydroxy-proline, N-methylglycine, allo-threonine, methyl-threonine, hydroxy-ethylcysteine, hydroxyethylhomo-cysteine, nitro-glutamine, homoglutamine, pipecolic acid, tert-leucine, norvaline, 2-azaphenylalanine, 3-azaphenyl-alanine, 4-azaphenyl-alanine, and 4-fluorophenylalanine. Several methods are known in the art for incorporating non-naturally occurring amino acid residues into proteins. For example, an in vitro system can be employed wherein nonsense mutations are suppressed using chemically aminoacylated suppressor tRNAs. Methods for synthesizing amino acids and aminoacylating tRNA are known in the art. Transcription and translation of plasmids containing nonsense mutations is carried out in a cell free system comprising an E. coli S30 extract and commercially available enzymes and other reagents. Proteins are purified by chromatography. See, for example, Robertson et al., J. Am. Chem. Soc. 113:2722, 1991; Ellman et al., Methods Enzymol. 202:301, 1991; Chung et al., Science 259:806-9, 1993; and Chung et al., Proc. Natl. Acad. Sci. USA 90:10145-9, 1993). In a second method, translation is carried out in Xenopus oocytes by microinjection of mutated mRNA and chemically aminoacylated suppressor tRNAs (Turcatti et al., J. Biol. Chem. 271:19991-8, 1996). Within a third method, E. coli cells are cultured in the absence of a natural amino acid that is to be replaced (e.g., phenylalanine) and in the presence of the desired non-naturally occurring amino acid(s) (e.g., 2-azaphenylalanine, 3-azaphenylalanine, 4-azaphenylalanine, or 4-fluorophenylalanine). The non-naturally occurring amino acid is incorporated into the polypeptide in place of its natural counterpart. See, Koide et al., Biochem. 33:7470-6, 1994.

(94) The polypeptides of the present invention can be produced using recombinant nucleic acid technologies. Thus, in one embodiment, a polypeptide (as described above) is a recombinant polypeptide.

(95) Cleavage of BoNTs into the Active Di-Chain Form

(96) The Clostridium bacteria release the neurotoxins as single-chain polypeptides each having 1251 to 1315 amino acids. Thereafter naturally occurring proteases cleave each of these proteins at a defined location into 2 chains each (nicking), the two chains however remaining interlinked by a disulphide-bridge. These di-chain (dual-chain) proteins are referred to as holotoxins (see Shone et al. (1985), Eur J Biochem 151, 75-82). The two chains have different functions, as described herein. According to the invention, Lys-C is used to cleave the single-chain polypeptide into the di-chain form. Cleavage can be determined by any appropriate technique, examples of which are known in the art. See, for example WO 1995/033850, which is herein incorporated by reference. For example, SDS-PAGE gels, stained for total protein, run under reducing and non-reducing conditions may be used to identify the presence of the single- and/or di-chain forms of the polypeptide of the invention.

(97) Targeting Moieties

(98) A polypeptide of the invention may comprise a targeting moiety (TM) which is adapted for binding of the polypeptide to a cell by binding of the TM directly to a target cell or by binding of the TM to a ligand or ligands that bind to a target cell. Typically a TM that binds directly to a cell does so by functionally interacting with a binding site on the cell surface to cause a physical association between the polypeptide of the invention and the surface of the target cell.

(99) Thereafter, the translocation component (H.sub.N domain or fragment or variant thereof) effects transport of the protease component (light chain or fragment or variant thereof) into the cytosol of the target cell. Finally, once inside, the light chain inhibits the exocytic fusion process of the target cell by cleaving SNARE protein present in the cytosol of the target cell. Thus, by inactivating the exocytic fusion apparatus of the target cell, the polypeptide of the invention inhibits secretion by the target cell (for example, inhibits secretion of neurotransmitters such as acetylcholine from a target cholinergic motor neuron).

(100) Targeting Moiety (TM) means any chemical structure that functionally interacts with a binding site to cause a physical association between the polypeptide of the invention and the surface of a target cell. The term TM embraces any molecule (i.e. a naturally occurring molecule, or a chemically/physically modified variant thereof) that is capable of binding to a binding site on the target cell, which binding site is capable of internalisation (e.g. endosome formation)also referred to as receptor-mediated endocytosis. The TM may possess an endosomal membrane translocation function, in which case separate TM and H.sub.N domain components need not be present in an agent of the present invention. Any appropriate TM may be used in a polypeptide of the present invention.

(101) In a preferred embodiment the TM is selected from the TM of a BoNT, i.e. the TM comprises or consists of a BoNT H.sub.CC domain. The term BoNT H.sub.CC domain as used herein embraces functional fragments and variants of BoNT H.sub.CC domains, i.e. H.sub.CC domain fragments and variants which retain the targeting activity of the full-length H.sub.CC domain. Alternatively, the TM may comprise or consist of a H.sub.CC domain from a non-botulinum clostridial toxin. All relevant disclosure herein in relation to BoNT H.sub.CC domain or H.sub.C domain TMs applies equally to non-botulinum H.sub.CC domain or H.sub.C domain TMs, including TeNT H.sub.CC domain or H.sub.C domain TMs.

(102) As the BoNT H.sub.CC domain is comprised within the larger BoNT H.sub.C domain, the TM of the polypeptide of the invention may comprise or consist of a BoNT H.sub.C domain. All description herein of a BoNT H.sub.CC domain therefore applies equally to the larger BoNT H.sub.C domain.

(103) The TM of the polypeptide of the invention may comprise or consist of a H.sub.CC domain or H.sub.C domain from any one of BoNT/A, BoNT/B, BoNT/C.sub.1, BoNT/D, BoNT/E, BoNT/F or BoNT/G. The H.sub.CC and H.sub.C domains of different BoNT serotypes and subtypes (sub-serotypes) are known in the art. See, for example, Table 1 of WO 2006/027207, or WO 2006/099590 herein incorporated by reference.

(104) Examples of clostridial toxin H.sub.CC domain reference sequences include:

(105) BoNT/Aamino acid residues 1092-1296, for example R1092 to L1296

(106) BoNT/Bamino acid residues 1079-1291, for example S1079 to E1291

(107) BoNT/C.sub.1amino acid residues 1093-1291, for example Q1093 to E1291

(108) BoNT/Damino acid residues 1080-1276, for example I1080 to E1276

(109) BoNT/Eamino acid residues 1067-1252, for example P1067-K1252

(110) BoNT/Famino acid residues 1087-1274, for example P1087-E1274

(111) BoNT/Gamino acid residues 1087-1297, for example S1087-E1297

(112) TeNTamino acid residues 1109-1315, for example L1109-D1315

(113) The above sequence positions may vary slightly according to serotype/subtype.

(114) Examples of clostridial toxin H.sub.C domain reference sequences include:

(115) BoNT/Aamino acid residues 872-1296, for example N872-L1296

(116) BoNT/Bamino acid residues 859-1291, for example E859-E1291

(117) BoNT/C.sub.1amino acid residues 867-1291, for example N867-E1291

(118) BoNT/Damino acid residues 863-1276, for example S863-E1276

(119) BoNT/Eamino acid residues 846-1252, for example R846-K1252

(120) BoNT/Famino acid residues 865-1274, for example K865-E1274

(121) BoNT/Gamino acid residues 864-1297, for example N864-E1297

(122) TeNTamino acid residues 880-1315, for example I880-D1315

(123) The above sequence positions may vary slightly according to serotype/subtype.

(124) In one embodiment, the polypeptide of the present invention comprising a TM that comprises or consists of a H.sub.CC domain or H.sub.C domain from a BoNT/F subtype. The BoNT/F H.sub.CC domain or H.sub.C domain may be from BoNT/F.sub.1, BoNT/F.sub.2, BoNT/F.sub.3, BoNT/F.sub.4, BoNT/F.sub.5, BoNT/F.sub.6, or BoNT/F.sub.7. For example, the H.sub.CC domain or H.sub.C domain may be from BoNT/F.sub.5, or BoNT/F.sub.7.

(125) The H.sub.CC domain or H.sub.C domain may be derived from the same BoNT/F subtype as the BoNT/F LH.sub.N backbone of the polypeptide of the invention. For example, if the BoNT/F LH.sub.N backbone is from a BoNT/F.sub.7 subtype, the TM may be a BoNT/F.sub.7 H.sub.CC domain or H.sub.C domain. Thus, in one embodiment, the polypeptide of the invention is identical to a single chain naturally occurring BoNT/F, except that the activation loop has been replaced by the activation loop from a different BoNT/F subtype (and hence, for the avoidance of doubt, the polypeptide of the invention is not a naturally-occurring BoNT).

(126) The BoNT/F H.sub.CC domain or H.sub.C domain TM of the invention may be a BoNT/F H.sub.CC domain or H.sub.C domain as identified in Table 1 herein. For example, as indicated above, in a preferred embodiment the H.sub.CC domain or H.sub.C domain is from BoNT/F.sub.7. In this instance, the H.sub.CC domain or H.sub.C domain may comprise or consist of the H.sub.CC domain or H.sub.C domain of a BoNT/F.sub.7 subtype, such as the BoNT/F.sub.7 of UPI0001DE3DAC or a fragment or variant thereof. As described herein, it is sufficient to refer to the H.sub.CC domain or H.sub.C domain of a BoNT/F.sub.7 such as UPI0001DE3DAC, or a fragment or variant thereof. Alternatively, the H.sub.CC domain or H.sub.C domain or fragment or variant thereof of BoNT/F.sub.7 (e.g. UPI0001DE3DAC) may be defined in terms of its sequence, or in terms of the amino acid corresponding to the amino acid positions in the full-length amino acid sequence of the BoNT/F.sub.7 (as described above). Using the non-limiting example of UPI0001DE3DAC, the full-length H.sub.CC domain may be referred to as amino acids corresponding to amino acid positions 1077 to 1268 (for the full-length H.sub.CC domain) or amino acid positions 1095 to 1260 (for the minimal H.sub.CC domain) of UPI0001DE3DAC (full-length UPI0001DE3DAC has the amino acid sequence of SEQ ID NO: 13)). Such definitions (in terms of sequence and/or amino acid position) apply equally to any other BoNT/F H.sub.CC domains or H.sub.C domains, including those listed in Table 1.

(127) Thus, in a preferred embodiment, the polypeptide of the invention comprises an amino acid sequence derived from a full-length BoNT/F.sub.5 or BoNT/F.sub.7 amino acid sequence, except that the BoNT/F.sub.5 or BoNT/F.sub.7 activation loop sequence has been replaced by an activation loop sequence from a BoNT/F subtype other than BoNT/F.sub.5 or BoNT/F.sub.7. In a particularly preferred embodiment, the BoNT/F.sub.5 or BoNT/F.sub.7 activation loop region is replaced by a BoNT/F.sub.1 activation loop region. For example, the polypeptide of the invention may comprise or consist of the amino acid sequence of SEQ ID NO: 75, or a variant thereof (as defined herein).

(128) The TM of the invention may be a modified clostridial H.sub.CC or H.sub.C domain, see, for example, WO 2006/027207, WO 2006/114308 and WO 2013/180799, each of which is incorporated by reference in its entirety.

(129) The term binding as used herein may mean that one molecule (in this instance the H.sub.CC or H.sub.C domain) is directly or indirectly contacting another molecule via at least one intermolecular or intramolecular force, including, without limitation, a covalent bond, an ionic bond, a metallic bond, a hydrogen bond, a hydrophobic interaction, a van der Waals interaction, and the like, or any combination thereof. Thus, binding affinity means the strength of a molecule (in this instance the H.sub.CC or H.sub.C domain) for a particular receptor.

(130) Alternatively, the polypeptide of the invention may be a re-targeted clostridial toxin, i.e. the polypeptide may comprise a BoNT/F LH.sub.N backbone and an BoNT/F activation loop as disclosed herein, but may comprise a non-clostridial TM.

(131) Examples of non-clostridial TMs are described, for example, in EP-B-0689459; WO 1994/021300; EP-B-0939818; U.S. Pat. Nos. 6,461,617; 7,192,596; WO 1998/007864; EP-B-0826051; U.S. Pat. Nos. 5,989,545; 6,395,513; 6,962,703; WO 1996/033273; EP-B-0996468; U.S. Pat. No. 7,052,702; WO 1999/017806; EP-B-1107794; U.S. Pat. No. 6,632,440; WO 2000/010598; WO 2001/21213; WO 2006/059093; WO 2000/62814; WO 2000/04926; WO 1993/15766; WO 2000/61192; WO 2005/023309; WO 2006/059113; WO 2006/059105; WO 2007/138339; WO 2009/150469; WO 2010/055358; WO 2009/150470; WO 2010/020811; WO 2010/094905; WO 2014/128497 and WO 1999/58571; all of which are hereby incorporated by reference in their entirety. References herein to TSIs and/or to non-clostridial TMs include structural equivalents such as those described in WO 2011/018665, which is herein incorporated by reference.

(132) Polynucleotides of the Invention

(133) The present invention also provides a polynucleotide that encodes the polypeptide of the invention. The term polynucleotide encompasses both DNA and RNA sequences, although typically the polynucleotide comprises a DNA sequence.

(134) The invention further provides an expression vector comprising a polynucleotide of the invention. Typically in the expression vector the polynucleotide of the invention is operably linked to a suitable promoter. The polynucleotide may also be linked to a suitable terminator sequence. Suitable promoter and terminator sequences are well known in the art.

(135) The choice of promoter depends in this case on the expression systems used for expression. In general, constitutive promoters are preferred, but inducible promoters may likewise be used. The construct produced in this manner includes at least one part of a vector, in particular regulatory elements, the vector, for example, being selected from A-derivates, adenoviruses, baculoviruses, vaccinia viruses, SV40-viruses and retroviruses. The vector is preferably capable of expressing the nucleic acid in a given host cell.

(136) The expression vector may a promoter selected from:

(137) TABLE-US-00003 Promoter Induction Agent Typical Induction Condition Tac (hybrid) IPTG 0.2 mM (0.05-2.0 mM) AraBAD L-arabinose 0.2% (0.002-0.4%) T7-lac operator IPTG 0.2 mM (0.05-2.0 mM)

(138) In another preferred embodiment, the vector has a promoter selected from:

(139) TABLE-US-00004 Promoter Induction Agent Typical Induction Condition Tac (hybrid) IPTG 0.2 mM (0.05-2.0 mM) AraBAD L-arabinose 0.2% (0.002-0.4%) T7-lac operator IPTG 0.2 mM (0.05-2.0 mM) T5-lac operator IPTG 0.2 mM (0.05-2.0 mM)

(140) The nucleic acid molecules of the invention may be made using any suitable process known in the art. Thus, the nucleic acid molecules may be made using chemical synthesis techniques. Alternatively, the nucleic acid molecules of the invention may be made using molecular biology techniques.

(141) The expression vector of the present invention is preferably designed in silico, and then synthesised by conventional polynucleotide synthesis techniques.

(142) The polynucleotide sequence information is optionally modified for codon-biasing according to the ultimate host cell (e.g. E. coli) expression system that is to be employed.

(143) The polynucleotide backbone is preferably screened for any inherent nucleic acid sequence, which when transcribed and translated would produce an amino acid sequence corresponding to the protease cleavage site encoded by the light-chain-coding sequence. This screening may be performed manually or with the assistance of computer software (e.g. the MapDraw program by DNASTAR, Inc.).

(144) The present invention also provides polypeptides encoded by polynucleotide sequences as described above.

(145) The percent sequence identity between two or more nucleic acid or amino acid sequences is a function of the number of identical positions shared by the sequences. Thus, % identity may be calculated as the number of identical nucleotides/amino acids divided by the total number of nucleotides/amino acids, multiplied by 100. Calculations of % sequence identity may also take into account the number of gaps, and the length of each gap that needs to be introduced to optimize alignment of two or more sequences. Sequence comparisons and the determination of percent identity between two or more sequences can be carried out using specific mathematical algorithms, such as BLAST, which will be familiar to a skilled person.

(146) Methods of Production

(147) The invention further provides a method of producing a polypeptide of the invention, said method comprising the step of expressing a polynucleotide of the invention in a cell and recovering the expressed polypeptide. The method typically further comprises a step of introducing the polypeptide of the invention into the cell. For example, the polynucleotide of the invention may be introduced into the cell in the form of an expression vector as described herein.

(148) For example, said method for producing a polypeptide of the invention may the comprise expressing a polynucleotide (as described above) in a suitable host cell, lysing the host cell to provide a host cell homogenate containing the polypeptide, and isolating the polypeptide.

(149) The polynucleotide provided/introduced to the cell may be DNA, RNA or mixtures thereof. The polynucleotide may furthermore be modified with regard to its nuclease resistance, such as e.g. by inserting phosphorthioate bonds. The nucleic acid may be produced from a starting nucleic acid, the latter being accessible e.g. by cloning from genomic or cDNA-databases. Moreover, the nucleic acid may be produced directly by solid phase synthesis. Suitable methods are known to the person skilled in the art. If one assumes a starting nucleic acid, a specific modification, e.g. by locality-specific mutagenesis, may be brought about, resulting in at least one addition, insertion, deletion and/or substitution on the amino acid level. The nucleic acid is then linked operatively to a suitable promoter as described herein.

(150) Typically the polypeptide of the invention from the cell is purified and/or concentrated after recovery from the cell. Any suitable method(s) may be used for the recovery, purification and/or concentration of the polypeptide of the invention. Standard techniques for recovery, purification and/or concentration are known in the art, for example chromatography methods and/or electrophoresis.

(151) The polypeptide of the invention may comprise one or more N-terminal and/or C-terminal located purification tags to assist in the purification of the polypeptide. Whilst any purification tag may be employed, the following are preferred: His-tag (e.g. 6 histidine) (SEQ ID NO: 126), preferably as a C-terminal and/or N-terminal tag; MBP-tag (maltose binding protein), preferably as an N-terminal tag; GST-tag (glutathione-S-transferase), preferably as an N-terminal tag; His-MBP-tag, preferably as an N-terminal tag; GST-MBP-tag, preferably as an N-terminal tag; Thioredoxin-tag, preferably as an N-terminal tag; and/or CBD-tag (Chitin Binding Domain), preferably as an N-terminal tag.

(152) One or more peptide spacer/linker molecules may be included in the polypeptide. For example, a peptide spacer may be employed between a purification tag and the rest of the polypeptide molecule.

(153) Activation Methods

(154) The polypeptides of the invention are prepared in single-chain form (see, for example, WO98/07864), and a proteolytic activation step is typically required for full activity. Therefore, the invention provides a method of activating a polypeptide of the invention by converting the polypeptide from the single-chain form to the di-chain form of the polypeptide. This technique is based on the in vivo bacterial mechanism by which native clostridial neurotoxin (i.e. holotoxin) is produced, and results in a polypeptide having the following simplified structural arrangement:

(155) NH2-[light chain]-[cleavage site]-[H.sub.N domain]-COOH

(156) Thus, typically the polypeptide is activated by treatment with a protease, which cleaves at the cleavage site (activation loop) between the light chain and the H.sub.N domain of the LH.sub.N backbone. A di-chain protein is thus produced, comprising the light chain as a single polypeptide chain covalently attached (via a disulphide bridge) to another single polypeptide chain containing the H.sub.N domain.

(157) Alternative structures of polypeptides of the invention are described, for example, in WO 2006/059113, WO06/059093, WO98/07864 and WO2012/156743, each of which is herein incorporated by reference in its entirety.

(158) Accordingly, the invention provides a method of activating a polypeptide of the invention, comprising incubating said polypeptide with a BoNT/F activation loop protease, wherein cleavage of the polypeptide at the BoNT/F cleavage site converts the polypeptide from a single-chain polypeptide into a di-chain polypeptide. Typically this conversion from a single-chain polypeptide into a di-chain polypeptide activates the polypeptide.

(159) The BoNT/F cleavage site (activation loop) may be cleaved by any appropriate enzyme (such as Lys-C). Lys-C, which is commercially available and may be isolated from Lysobacter enzymogenes), is preferred.

(160) Activated Di-Chain Polypeptides

(161) As described herein, activated BoNT are di-chain polypeptides, produced by enzymatic cleavage within the activation loop. The present inventors have conducted N-terminal sequencing on the activated di-chain form of the chimeric BoNT/F.sub.7-1 described herein (see the Examples) and have found that the chain comprising the H.sub.N domain has a unique N-terminal amino acid sequence not found in either the wild-type (unmodified) BoNT/F.sub.1 or BoNT/F.sub.7 subtypes.

(162) In particular, the inventors have found that the chain comprising the H.sub.N domain comprises the N-terminal amino acid sequence APPR (SEQ ID NO: 127), more specifically APPRLCIKV (SEQ ID NO: 74). Thus, the activated di-chain form of chimeric BoNT/F.sub.7-1 comprising a chain comprising the H.sub.N domain which additionally includes some amino acids from the BoNT/F.sub.1 activation loop as well as amino acids from the H.sub.N domain of BoNT/F.sub.7 (see FIG. 8).

(163) Accordingly, the present invention provides a chimeric di-chain BoNT/F polypeptide comprising: (a) a light chain polypeptide sequence as disclosed herein and (b) a H.sub.N polypeptide sequence as disclosed herein; wherein the N-terminus of the H.sub.N polypeptide comprises amino acid residues from a different BoNT/F subtype than the remainder of the H.sub.N polypeptide. Typically the N-terminal amino acid residue of the H.sub.N polypeptide is A or Q. For example, the N-terminus of the H.sub.N polypeptide may begin with an amino acid motif selected from APPR (SEQ ID NO: 127) or QSPS (SEQ ID NO: 128). In a preferred embodiment, the N-terminus of the H.sub.N polypeptide begins with an amino acid motif selected from APPRLCIK (SEQ ID NO: 70), APPRLCIT (SEQ ID NO: 71), QSPSLCIK (SEQ ID NO: 72) and QSPSLCIT (SEQ ID NO: 73).

(164) In a preferred embodiment, the present invention provides a chimeric di-chain BoNT/F polypeptide comprising: (a) a (BoNT/F) light chain polypeptide sequence having at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of amino acids corresponding to residues 1 to 430 of SEQ ID NO: 1 (such as amino acid residues from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 1 to 430 of SEQ ID NO: 1); and/or amino acid residues corresponding to residues 15 to 412 of any one of SEQ ID NOs: 1 to 16, or an amino acid sequence comprising or consisting of amino acids positions from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 15 to 412 of SEQ ID NO: 1; and/or an amino acid sequence which comprises or consists of any one of SEQ ID NOs: 33 to 48, preferably SEQ ID NO: 45; and (b) a (BoNT/F) H.sub.N domain polypeptide sequence having at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of amino acids corresponding to residues 520 to 873 of any one of SEQ ID NOs: 1 (such as amino acid residues from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 520 to 873 of SEQ ID NO: 1); and/or an amino acid sequence comprising or consisting of amino acid positions from any one of SEQ ID NOs: 2 to 16, preferably from SEQ ID NO: 13, which correspond to amino acid residues 520 to 873 of SEQ ID NO: 1; and/or an amino acid sequence which comprises or consists of any one of SEQ ID NOs: 49 to 64, preferably SEQ ID NO: 61; wherein the N-terminus of the H.sub.N polypeptide comprises amino acid residues from a different BoNT/F subtype than the remainder of the H.sub.N polypeptide.

(165) In a preferred embodiment, the chimeric di-chain BoNT/F polypeptide of the invention comprises a BoNT/F.sub.7 or BoNT/F.sub.5 light chain or light chain fragment and a BoNT/F.sub.7 or BoNT/.sub.5 H.sub.N domain or H.sub.N domain fragment. Thus, in a preferred embodiment, the chimeric di-chain BoNT/F polypeptide of the invention comprises: (a) a (BoNT/F) polypeptide sequence having at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13, which corresponds to amino acids 1 to 430 of SEQ ID NO: 1, such as amino acid residues 1 to 421 of any one of SEQ ID NOs: 13 to 16, preferably SEQ ID NO: 13, or amino acid residues 1 to 428 of SEQ ID NO: 8; and/or an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13, which corresponds to amino acids 15 to 412 of SEQ ID NO: 1, such as amino acid residues 15 to 403 of any one of SEQ ID NOs: 13 to 16, preferably SEQ ID NO: 13, or amino acid residues 15 to 410 of SEQ ID NO: 8; and/or any one of SEQ ID NOs: 40 or 45 to 48, preferably SEQ ID NO: 45; and (b) at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or more, up to 100% amino acid sequence identity with an amino acid sequence comprising or consisting of an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13, which corresponds to amino acids 520 to 873 of SEQ ID NO: 1, such as amino acid residues 509 to 862 of any one of SEQ ID NOs: 13 to 16, preferably SEQ ID NO: 13, or amino acid residues 516 to 868 of SEQ ID NO: 8; and/or an amino acid sequence from any one of SEQ ID NOs: 8 or 13 to 16, preferably from SEQ ID NO: 13, which corresponds to amino acids 538 to 850 of SEQ ID NO: 1, such as amino acid residues 527 to 839 of any one of SEQ ID NOs: 13 to 16, preferably SEQ ID NO: 13, or amino acid residues 534 to 845 of SEQ ID NO: 8, and/or any one of SEQ ID NOs: 56 or 61 to 64, preferably SEQ ID NO: 61; wherein the N-terminus of the H.sub.N polypeptide comprises amino acid residues from a different BoNT/F subtype than the remainder of the H.sub.N polypeptide.

(166) In a particularly preferred embodiment, the invention provides a chimeric di-chain BoNT/F polypeptide comprising: (a) a light chain polypeptide comprising an amino acid sequence having at least 40% sequence identity at least one of SEQ ID NOs: 33 to 48, preferably SEQ ID NO: 45; and (b) an H.sub.N polypeptide comprising an amino acid sequence having at least 70% sequence identity at least one of SEQ ID NOs: 49 to 64, preferably SEQ ID NO: 61; wherein the N-terminus of the H.sub.N polypeptide comprises amino acid residues from a different BoNT/F subtype than the remainder of the H.sub.N polypeptide.

(167) Typically the N-terminal amino acid residue of the H.sub.N polypeptide is A or Q. For example, the N-terminus of the H.sub.N polypeptide may begin with an amino acid motif selected from APPR (SEQ ID NO: 127) or QSPS (SEQ ID NO: 128). In a preferred embodiment, the N-terminus of the H.sub.N polypeptide begins with an amino acid motif selected from APPRLCIK (SEQ ID NO: 70), APPRLCIT (SEQ ID NO: 71), QSPSLCIK (SEQ ID NO: 72) and QSPSLCIT (SEQ ID NO: 73).

(168) A chimeric di-chain of the present invention may comprise a light chain polypeptide sequence from any BoNT/F subtype, preferably from BoNT/F.sub.5 or BoNT/F.sub.7. A chimeric di-chain of the present invention may comprise a H.sub.N polypeptide sequence from any BoNT/F subtype, preferably from BoNT/F.sub.5 or BoNT/F.sub.7. The light chain polypeptide sequence and the H.sub.N polypeptide sequence may be from the same BoNT/F subtype. For example, both the light chain polypeptide sequence and the H.sub.N polypeptide sequence may be from BoNT/F.sub.5 or BoNT/F.sub.7. The light chain polypeptide sequence and the H.sub.N polypeptide sequence may be from closely related subtypes. For example, the light chain polypeptide sequence may be from BoNT/F.sub.5 and the H.sub.N polypeptide sequence may be from BoNT/F.sub.7 or vice versa. In a preferred embodiment, the light chain polypeptide sequence and/or the H.sub.N polypeptide sequence is from a BoNT/F.sub.5 subtype or a BoNT/F.sub.7 subtype. In an even more preferred embodiment, both the light chain polypeptide sequence and the H.sub.N polypeptide sequence are from a BoNT/F.sub.5 subtype or a BoNT/F.sub.7 subtype, most preferably from a BoNT/F.sub.7 subtype. The light chain polypeptide sequence and the H.sub.N polypeptide sequence may be from different BoNT/F subtypes. Typically the light chain polypeptide sequence and the H.sub.N polypeptide sequence are from the same or closely related BoNT/F subtypes.

(169) A chimeric di-chain of the present invention comprises one or more amino acid residues from the activation loop of a different BoNT/F subtype than the BoNT/F subtype of the light chain polypeptide sequence and/or the H.sub.N polypeptide sequence. The one or more amino acids from a different BoNT/F subtype may be retained at the C-terminus of the light chain polypeptide sequence and/or the N-terminus of the H.sub.N polypeptide sequence. Typically the H.sub.N polypeptide sequence of the chimeric di-chain polypeptide of the invention is a BoNT/F.sub.5 or BoNT/F.sub.7 sequence and its N-terminus comprises one or more amino acids from a BoNT/F.sub.1, BoNT/F.sub.2, BoNT/F.sub.3, BoNT/F.sub.4 or BoNT/F.sub.6 activation loop, as described herein. In a preferred embodiment, the one or more amino acids are from a BoNT/F.sub.1 activation loop, as described herein.

(170) The chimeric di-chain polypeptide of the invention may comprise all the amino acids of the corresponding chimeric single-chain polypeptide, i.e. the sequence of the di-chain polypeptide may be identical to that of the single-chain polypeptide, except that it is split across two polypeptide chains, rather than being in a single chain. Alternatively, the chimeric di-chain polypeptide of the invention may be missing one or more amino acids compared with the corresponding chimeric single-chain polypeptide. Typically this arises due to cleavage at a second (or subsequent) site within the activation loop, resulting in loss of a short fragment of the activation loop sequence from the activated chimeric di-chain polypeptide. Thus, the chimeric di-chain polypeptide may be missing at least one, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten, at least eleven or more amino acids compared with the corresponding chimeric single-chain polypeptide.

(171) Any of the disclosure herein regarding the definition of the light chain polypeptide sequences and H.sub.N polypeptide sequences in relation to the single-chain chimeric polypeptide of the invention applies equally to these sequences within the chimeric di-chain polypeptide of the invention. For example, the light chain polypeptide sequence and/or the H.sub.N polypeptide sequence in the di-chain polypeptide of the invention is preferably from a BoNT/F.sub.5 subtype or a BoNT/F.sub.7 subtype. Any disclosure herein relating to TMs of the invention applies equally to the di-chain polypeptide as to the single-chain chimeric polypeptide.

(172) The di-chain polypeptide of the invention is typically obtainable from the single-chain chimeric polypeptide of the invention as described herein.

(173) Cells

(174) The polypeptide of the present invention may be produced by a suitable cell (also known as host cells), such as e.g. Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris or Bacillus megaterium, which multiplies a recombinant expression vector, the vector coding for a polypeptide of the invention.

(175) Accordingly, the invention further provides cells, which express a polypeptide of the invention. Typically the cells of the invention contain a polynucleotide or expression vector of the invention and are suitable for expressing the polynucleotide or vector. Numerous prokaryotic and eukaryotic expression systems are known in the state of the art, the cells being selected, for example, from prokaryotic cells such as E. coli or B. megaterium, from eukaryotic cells such as S. cerevisiae and P. pastoris. Although higher eukaryotic cells, such as insect cells or mammal cells, may be used as well, cells are nevertheless preferred, which, like C. botulinum, do not possess a glycosylation apparatus.

(176) Compositions and Therapeutic/Cosmetic Indications

(177) The invention also provides a pharmaceutical composition comprising a polypeptide or di-chain polypeptide of the invention. The pharmaceutical composition may optionally comprise a pharmaceutically acceptable excipient, diluent, carrier, propellant, salt and/or additive. Typically the pharmaceutical composition of the invention is suitable for treating or preventing diseases or disorders in which a therapy with a botulinum neurotoxin is indicated. The pharmaceutical composition is particularly suitable for subcutaneous, intramuscular, implant and/or topical administration. Intramuscular administration is preferred. Compositions suitable for injection may be in the form of solutions, suspensions or emulsions, or dry powders which are dissolved or suspended in a suitable vehicle prior to use.

(178) The invention also provides a cosmetic composition comprising a polypeptide or di-chain polypeptide of the invention. The cosmetic composition may optionally comprise a cosmetically acceptable excipient, diluent, carrier, propellant, salt and/or additive. Typically the cosmetic composition of the invention is suitable for alleviating a cosmetic indication for which the application of a botulinum neurotoxin is indicated.

(179) The cosmetic composition is particularly suitable for subcutaneous, intramuscular, implant and/or topical administration. Intramuscular or subcutaneous administration are preferred. Compositions suitable for injection may be in the form of solutions, suspensions or emulsions, or dry powders which are dissolved or suspended in a suitable vehicle prior to use.

(180) In the case of polypeptide, di-chain polypeptide or composition that is to be delivered locally, the engineered clostridial toxin may be formulated as a cream (e.g. for topical application), or for sub-dermal injection. Topical delivery means may include transdermal delivery (e.g. via an adhesive patch).

(181) Local delivery of either pharmaceutical or cosmetic compositions of the invention is also envisaged. The term local delivery typically indicates that the site of action is at (or near to the site of application). As a non-limiting example, a BoNT injected into a muscle to be weakened would be local delivery to that muscle by intramuscular injection.

(182) Systemic delivery of either pharmaceutical or cosmetic compositions of the invention is also envisaged. The term systemic delivery typically indicates that the site of action is not at (or near to) the site of action, but rather the BoNT is transported (usually in the blood) from where it is applied to where it acts. As a non-limiting example, BoNT injected into the general circulation with the intention of weakening a particular muscle, for which it has specificity, would be systemic delivery.

(183) Polypeptides, di-chain polypeptides and/or compositions of the invention may be administered to a patient by intrathecal or epidural injection in the spinal column at the level of the spinal segment involved in the innervation of an affected organ.

(184) A preferred route of administration is via laparoscopic and/or localised, particularly intramuscular, injection.

(185) The dosage ranges for administration of the polypeptides, di-chain polypeptides or compositions of the present invention are those to produce the desired therapeutic or cosmetic effect. It will be appreciated that the dosage range required depends on the precise nature of the polypeptide, di-chain polypeptide or composition, the route of administration, the nature of the formulation, the age of the patient, the nature, extent or severity of the patient's condition, contraindications, if any, and the judgement of the attending physician. Variations in these dosage levels can be adjusted using standard empirical routines for optimisation.

(186) Suitable daily dosages (per kg weight of patient) are in the range 0.001-10 ng/kg, preferably 0.001-5 ng/kg, more preferably 0.02-5 ng/kg, and particularly preferably 0.04-5 ng/kg. The unit dosage can vary from less than 10 picogram to 300 ng, but typically will be in the region of 0.1 to 10 ng per dose, which may be administered daily or preferably less frequently, such as weekly or six monthly.

(187) A particularly preferred dosing regimen is based on 0.5 ng of polypeptide/di-chain polypeptide as the 1 dose. In this regard, preferred dosages are in the range 1-100 (i.e. 0.5-50 ng).

(188) Fluid dosage forms are typically prepared utilising the polypeptide/di-chain polypeptide and a pyrogen-free sterile vehicle. The polypeptide or di-chain polypeptide, depending on the vehicle and concentration used, can be either dissolved or suspended in the vehicle. In preparing solutions the polypeptide or di-chain polypeptide can be dissolved in the vehicle, the solution being made isotonic if necessary by addition of sodium chloride and sterilised by filtration through a sterile filter using aseptic techniques before filling into suitable sterile vials or ampoules and sealing. Alternatively, if solution stability is adequate, the solution in its sealed containers may be sterilised by autoclaving. Advantageously additives such as buffering, solubilising, stabilising, preservative or bactericidal, suspending or emulsifying agents and or local anaesthetic agents may be dissolved in the vehicle.

(189) Dry powders, which are dissolved or suspended in a suitable vehicle prior to use, may be prepared by filling pre-sterilised ingredients into a sterile container using aseptic technique in a sterile area. Alternatively the ingredients may be dissolved into suitable containers using aseptic technique in a sterile area. The product is then freeze dried and the containers are sealed aseptically.

(190) Parenteral suspensions, suitable for intramuscular, subcutaneous or intradermal injection, are prepared in substantially the same manner, except that the sterile components are suspended in the sterile vehicle, instead of being dissolved and sterilisation cannot be accomplished by filtration. The components may be isolated in a sterile state or alternatively it may be sterilised after isolation, e.g. by gamma irradiation.

(191) A suspending agent, for example, polyvinylpyrrolidone may be included in the composition(s) to facilitate uniform distribution of the components.

(192) Administration in accordance with the present invention may take advantage of a variety of delivery technologies including microparticle encapsulation, viral delivery systems or high-pressure aerosol impingement.

(193) The invention further provides a polypeptide, di-chain polypeptide and/or pharmaceutical composition as described herein for use in a method of preventing or treating a disease or disorder for which a therapy with a botulinum neurotoxin is indicated. The invention further provides the use of a polypeptide, di-chain polypeptide and/or pharmaceutical composition as described herein in the manufacture of a medicament for preventing or treating a disease or disorder for which a therapy with a botulinum neurotoxin is indicated. The invention further provides a method of treating a disease or disorder for which a therapy with a botulinum neurotoxin is indicated comprising administering a pharmaceutically effective dose of a polypeptide, di-chain polypeptide and/or pharmaceutical composition as described herein to an individual in need thereof. The disease or disorder may be any disease or disorder as described herein.

(194) The invention further provides a BoNT/F.sub.7-1 polypeptide and/or pharmaceutical composition comprising a BoNT/F.sub.7-1 polypeptide for use in a method of preventing or treating a disease or disorder for which a therapy with a botulinum neurotoxin is indicated. The invention further provides the use of a BoNT/F.sub.7-1 polypeptide and/or pharmaceutical composition comprising a BoNT/F.sub.7-1 polypeptide in the manufacture of a medicament for preventing or treating a disease or disorder for which a therapy with a botulinum neurotoxin is indicated. The invention further provides a method of treating a disease or disorder for which a therapy with a botulinum neurotoxin is indicated comprising administering a pharmaceutically effective dose of a BoNT/F.sub.7-1 polypeptide and/or pharmaceutical composition comprising a BoNT/F.sub.7-1 polypeptide to an individual in need thereof. The disease or disorder may be any disease or disorder as described herein. The BoNT/F.sub.7-1 polypeptide is preferable a di-chain (activated) BoNT/F.sub.7-1 polypeptide.

(195) The invention further provides the use of a polypeptide, di-chain polypeptide and/or cosmetic composition as described herein for preventing or alleviating a cosmetic indication for which the application of a botulinum neurotoxin is indicated. The cosmetic indication may be any cosmetic indication as described herein.

(196) The invention further provides the use of a BoNT/F.sub.7-1 polypeptide and/or cosmetic composition comprising a BoNT/F.sub.7-1 polypeptide for preventing or alleviating a cosmetic indication for which the application of a botulinum neurotoxin is indicated. The cosmetic indication may be any cosmetic indication as described herein. The BoNT/F.sub.7-1 polypeptide is preferable a di-chain (activated) BoNT/F.sub.7-1 polypeptide.

(197) Any reference herein to therapeutic and/or cosmetic indications, or to pharmaceutical and/or cosmetic compositions applies equally to the single-chain chimeric polypeptides of the inventions and the di-chain polypeptides described herein.

EXAMPLES

(198) The following Examples serve to illustrate particular embodiments of the invention, and do not limit the scope of the invention defined in the claims in any way.

Example 1Alignment of the Activation Loop Amino Acid Sequences Across all BoNT Serotypes and Subtypes

(199) The UniParc database was searched, by a basic local alignment search tool (BLAST) search for full-length BoNT proteins. The amino acid sequences of all full-length BoNTs identified in the BLAST search were analysed and the activation loop sequences identified. The activation loop sequences of all BoNT/A and BoNT/F subtypes were aligned (FIG. 1) and analysed for any significant similarities and differences.

(200) It was surprisingly found that, although there was a high degree of intraserotype conservation of the activation loops sequences between the BoNT/A sequences, the level of conservation between the BoNT/F subtypes was much lower. In particular, it was found that the activation loops from BoNT/F.sub.5 and BoNT/F.sub.7 subtypes were particularly divergent compared with other BoNT/F subtypes (FIGS. 1, 3 and 4).

Example 2Preparation of an Expression Plasmid Encoding Chimeric BoNT/F.SUB.7-1

(201) Phylogenetic analysis of the different BoNT/F subtypes was conducted (FIG. 2) to identify a BoNT/F.sub.1 subtype and a BoNT/F.sub.7 subtype for the generation of a chimeric protein BoNT/F.sub.7-1.

(202) The naturally occurring protein, UniParc number UPI00000B66D1, is subtype F.sub.1 (BoNT/F.sub.1). This BoNT/F.sub.1 was prepared as a recombinant protein and named SXN102691, recombinant BoNT/F1 (rBoNT/F.sub.1).

(203) The naturally occurring protein, UniParc number UPI0001DE3DAC, aligns together with three other, highly similar proteins, into subtype F.sub.7 (BoNT/F.sub.7). This BoNT/F.sub.7 was prepared as a recombinant protein and named SXN102697, recombinant BoNT/F.sub.7 (rBoNT/F.sub.7).

(204) rBoNT/F.sub.1 and rBoNT/F.sub.7 were purified as single chain precursor proteins. Conditions were identified for post-translational modification of purified single chain rBoNT/F.sub.1 by selective proteolysis by the protease Lys-C. This created the mature di-chain form of the rBoNT/F.sub.1 protein. The post-translational proteolysis step is called activation because the mature (activated) forms of BoNT proteins show higher specific activity in functional assays compared to the single chain forms.

(205) In contrast to rBoNT/F.sub.1, no conditions to selectively activate rBoNT/F.sub.7 could be identified. Instead, under all conditions tested rBoNT/F.sub.7 proteolysis was not selective, but rather occurred at several additional sites in the protein. Proteolysis at unwanted sites is called degradation.

(206) The overall structure of BoNT proteins, including the location of the selectively proteolysed site at which they become activated, is well conserved. Conservation between BoNTs is especially apparent at the levels of secondary and tertiary structures. Therefore it was reasoned that substituting the activation loop from rBoNT/F.sub.1 into the structure of rBoNT/F.sub.7 would create a chimeric protein that both retained the functional characteristic of rBoNT/F.sub.7 and was also amenable to post-translational activation like rBoNT/F.sub.1. We called this chimeric protein rBoNT/F.sub.71 (FIG. 5).

(207) The chimeric protein rBoNT/F.sub.1-7 is composed of amino acids 1-421 from UPI0001DE3DAC (BoNT/F.sub.7), 430-444 from UPI00000B66D1 (BoNT/F.sub.1), and 435-1268 from UPI0001DE3DAC (BoNT/F.sub.7) (see FIG. 6 for full amino acid sequences). The chimeric protein rBoNT/F.sub.7-1 is named SXN102875. The percentage identity and divergence of rBoNT/F.sub.1, rBoNT/F.sub.7 and rBoNT/F.sub.7-1 is shown in Table 2 below.

(208) TABLE-US-00005 TABLE 2 Percent Identity rBoNT/F.sub.7-1 rBoNT/F.sub.7 rBoNT/F.sub.1 Divergence rBoNT/F.sub.7-1 99.5 74.9 rBoNT/F.sub.7 0.5 74.4 rBoNT/F.sub.1 30.6 31.3

(209) An expression plasmid, encoding chimeric BoNT/F.sub.7-1, was created by two rounds of PCR based site-directed mutagenesis (SDM), to modify the codons for the activation loop region of BoNT/F.sub.7 (in our existing expression plasmid for SXN102697) to encode the amino acid sequence of the activation loop region from BoNT/F.sub.1. The existing expression plasmid for SXN102697 was made using chemical synthesis techniques and contains an open reading frame which encodes BoNT/F.sub.7 (SEQ ID NO: 13BoNT/F.sub.7UniParc Accession No. UPI0001DE3DAC), and is codon-optimised for efficient protein expression in E. coli bacteria cells.

(210) TABLE-US-00006 TheaminoacidsequenceoftheBoNT/F.sub.1activation loopis: (SEQIDNO:66) KSVIPRKGTKAPPRL TheaminoacidsequenceoftheBoNT/F.sub.7activation loopis: (SEQIDNO:69) KSIVSKKGTKN--SL
Site directed mutagenesis (SDM) primers were:

(211) TABLE-US-00007 TABLE3 Primer name Sequence %GC F7_1_For 5-CcaagAAGGGTACGAAAGCACCGCC 53.2 TCGCCTGTGCATTAAAGTCAAC-3 F7_1_Rev 5-gtTgactTtaAtgcaCagGcgaGgc 53.2 ggTgCtTtCgtaccCttcttgg-3 F7_2_For 5-CtTcGtgggcctgtgcAaaagcgTT 54.0 attCcgcgtaagggtAcgaaAgCaC-3 F7_2_Rev 5-gtgcTTtcgtaCCcTtacgcGgAat 54.0 AacgcTTTtgcacaGgCCcacgAag-3
First Round of Site Directed Mutagenesis (SDM)

(212) The template plasmid for the first round of SDM was A002697 (and expression plasmid encoding BoNT/F.sub.7). Four independent reactions were set up for the first round of SDM:

(213) TABLE-US-00008 Reaction 1 Reaction 2 Template 1 l Template 1 l 10 pFU turbo reaction 5 l 10 pFU turbo reaction 5 l buffer buffer F7_1_For primer (10 uM) 2.5 l F7_1_For primer (10 uM) 2.5 l F7_1_Rev primer (10 uM) 2.5 l F7_1_Rev primer (10 uM) 2.5 l dNTP mix (10 mM each) 5 l dNTP mix (10 mM each) 5 l dH.sub.2O 33 l dH.sub.2O 30 l pFU polymerase 1 l MgCl.sub.2 (50 mM) 2.5 l pFU polymerase 1 l Reaction 3 Reaction 4 Template 0.5 l Template 0.5 l 10 pFU turbo reaction 5 l 10 pFU turbo reaction 5 l buffer buffer F7_1_For primer (10 M) 2.5 l F7_1_For primer (10 M) 2.5 l F7_1_Rev primer (10 M) 2.5 l F7_1_Rev primer (10 M) 2.5 l dNTP mix (10 mM each) 5 l dNTP mix (10 mM each) 5 l dH.sub.2O 33 l dH.sub.2O 31 l pFU polymerase 1 l MgCl.sub.2 (50 mM) 2.5 l pFU polymerase 1 l
PCR cycling conditions for all reactions were as follows:

(214) TABLE-US-00009 95 C. 30 sec 95 C. 30 sec 55 C. 1 min {close oversize brace} 18 cycles 72 C. 5 min 72 C. 10 min 4 C. hold

(215) The PCR reaction products were digested with DpnI, for 2 hours at 37 C., and then transformed into Top10 cells (Life Technologies, one shot cells Lot 1419720). The DpnI digestion reaction mix was:

(216) TABLE-US-00010 NEB Buffer 4 (10) 5 l PCR reaction mix 44 l Dpnl 1 l

(217) The transformations into Top10 cells were performed according to the manufacturer's instructions. Briefly, cells were defrosted on ice then 5 l digestion reaction was added to 50 l competent cells and incubated on ice for 30 min. Then the cells were heat shocked for 30 sec at 42 C. and returned to ice for a further 2 min. Then 200 l SOC media (supplied with the competent cells) was added to the transformation reaction and the cells were incubated for 1 hour at 37 C. in a shaking incubator. Transformed cells were plated onto agar-kanamycin plates and incubated overnight at 37 C.

(218) Reaction 1 resulted in 34 colonies

(219) Reaction 2 resulted in 122 colonies

(220) Reaction 3 resulted in 59 colonies

(221) Reaction 4 resulted in 42 colonies

(222) A pUC positive control transformation, performed alongside, resulted in 92 colonies.

(223) A single colony was picked from each of the four reaction conditions, grown overnight (10 ml L-Broth, kanamycin), and the plasmid DNA was harvested by Wizard Plus Miniprep (Promega), according to the manufacturer's instructions.

(224) Plasmid DNA from each of the 4 reaction conditions was sent for DNA sequencing, which confirmed that the expected point mutations had been inserted in all four cases.

(225) Second Round of Site Directed Mutagenesis (SDM)

(226) The template plasmid for the second round of SDM was plasmid DNA from the first round (reaction 2). Five different reactions were set up:

(227) TABLE-US-00011 Reaction 1 Reaction 2 Template 0.5 l Template 0.5 l 10 pFU turbo reaction 5 l 10 pFU turbo reaction 5 l buffer buffer F7_1_For primer (10 M) 2.5 l F7_1_For primer (10 M) 2.5 l F7_1_Rev primer (10 M) 2.5 l F7_1_Rev primer (10 M) 2.5 l dNTP mix (10 mM each) 5 l dNTP mix (10 mM each) 5 l dH.sub.2O 33 l dH.sub.2O 31 l pFU polymerase 1 l MgCl.sub.2 (50 mM) 2.5 l pFU polymerase 1 l Reaction 3 Reaction 4 Template 1 l Template 1 l 10 pFU turbo reaction 5 l 10 pFU turbo reaction 5 l buffer buffer F7_1_For primer (10 M) 2.5 l F7_1_For primer (10 M) 2.5 l F7_1_Rev primer (10 M) 2.5 l F7_1_Rev primer (10 M) 2.5 l dNTP mix (10 mM each) 5 l dNTP mix (10 mM each) 5 l dH.sub.2O 33 l dH.sub.2O 30 l pFU polymerase 1 l MgCl.sub.2 (50 mM) 2.5 l pFU polymerase 1 l Reaction 5 (negative control) Template 0.5 l 10 pFU turbo reaction buffer 5 l F7_2_For primer (10 M) 2.5 l F7_2_Rev primer (10 M) 2.5 l dNTP mix (10 mM each) 5 l dH.sub.2O 35 l pFU polymerase none
PCR Cycling Conditions for all Reactions

(228) TABLE-US-00012 95 C. 30 sec 95 C. 30 sec 55 C. 1 min {close oversize brace} 18 cycles 72 C. 5 min 72 C. 10 min 4 C. hold

(229) The PCR reaction products were digested with DpnI then transformed into Top10 cells as described for round 1

(230) Reaction 1 resulted in 13 colonies

(231) Reaction 2 resulted in 16 colonies

(232) Reaction 3 resulted in 15 colonies

(233) Reaction 4 resulted in 0 colonies

(234) Reaction 5 resulted in 0 colonies

(235) One colony each from reactions 1 and 2, and 2 from reaction 3, were grown overnight; DNA was extracted and sent for sequencing as described above.

(236) After conformation that the desired sequence encoding protein BoNT/F.sub.7-1 was present the sequence-verified DNA was transformed into competent BL21 (DE3) cells (Invitrogen), according to the manufacturer's instructions, and cell banks were laid down.

Example 3Purification of rBoNT/F.SUB.7-1

(237) Lysis by Cell Disruptor/Homogeniser

(238) E. coli cells, expressing rBoNT/F.sub.7-1, were harvested by centrifugation and then resuspended in 3 ml/g of lysis buffer (50 mM Tris pH 8.0, 200 mM NaCl), supplemented with 16 ul Benzonase (90% purity, 25 U/l). The resuspended cells were held on ice for 5 minutes before lysis.

(239) The cells were lysed by sonication (Misonix 3000 sonicator, 30 sec bursts with 45 sec rests, 8 cycles). The sonicated samples were transferred to a disposable 500 ml centrifuge pots (Corning, #431123), securely capped and the lysate clarified by centrifugation at 4300 g for 1 hour at 4 C.

(240) Butyl Sepharose High Performance Capture Step

(241) The cleared lysate was adjusted to 50 mM Tris pH 8.0, 100 mM NaCl, 1.35 M (NH.sub.4).sub.2SO.sub.4 by the addition of solid (powder) (NH.sub.4).sub.2SO.sub.4. The (NH.sub.4).sub.2SO.sub.4 was added in small increments over 10 minutes with discontinuous mixing. The sample was then decanted into a 500 ml corning tube, balanced and cleared by centrifugation at 4300 g for 1 hour at 20 C. The cleared lysate was then captured on 2 Butyl sepharose high performance HiTrap columns (10 ml resin) equilibrated in equilibration/wash buffer (50 mM Tris pH 8.0, 1.35 M (NH.sub.4).sub.2SO.sub.4). The column was loaded with sample (200 ml), washed for 10 column volumes with equilibration/wash buffer, then eluted over 25 column volumes from 50 mM Tris pH 8.0, 1.35 M (NH.sub.4).sub.2SO.sub.4 to 50 mM Tris pH 8.0. 5 ml fractions were collected throughout the gradient; 25 l samples from each fraction were added to 25 l of 4LDS buffer, made up to 100 l with dH.sub.2O then run on SDS-PAGE. Fractions containing the target protein were pooled.

(242) Desalting rBoNT/F.sub.7-1 into Low Salt Q Binding Buffer

(243) The pooled material from the Butyl HP capture step was applied to a pre-equilibrated HiPrep 26/10 desalting column (53 ml column, equilibrated in 50 mM Tris pH 8.5). The sample was then loaded at a flow rate of 8 ml/min in batches of 12 ml before the column was re-equilibrated with buffer. Protein eluting at lower conductivity <2 mSv/cm was collected as one pool.

(244) Intermediate Purification Step HiScreen Q High Performance

(245) The pooled material from the desalting step was loaded onto a pre-equilibrated Q HiScreen HP column (4.7 ml) at a flow rate of 1.2 ml/min. The column was equilibrated in 50 mM Tris pH 8.5. The column was washed with 3 column volumes of 10 mM Tris pH 8.5 at a flow rate of 1.2 ml/min. The protein was then eluted across a linear gradient: 20 column volumes from 50 mM Tris pH 8.5 to 50 mM Tris pH 8.5, 300 mM NaCl; followed by a 5 column volume high salt wash of 10 mM Tris pH 8.5, 1 M NaCl. The elution was completed at a flow rate of 1.2 ml/min and 2.5 ml fractions were collected throughout the elution. 25 l samples of the load, flow through, fractions were taken for analysis by SDS-PAGE.

(246) SDS-PAGE samples were prepared as follows 25 l fraction sample, 25 l LDS-buffer and 50 l dH.sub.2O. The fractions were mixed, spun down, boiled for 5 minutes, mixed and spun down again before loading onto a gel, confirming that purified rBoNT/F.sub.7-1 had been obtained (FIG. 7).

(247) Protein Concentration and Storage

(248) The protein was concentrated using a 6 ml vivaspin ultrafiltration column (50 kda MWCO). This was achieved by centrifugation at 4300 g until the retentate volume had decreased to give a suitable concentration. The concentration was then determined on a nanodrop lite and final values were derived from this.

Example 4Activation of rBoNT/F.SUB.7-1

(249) 7 ml of purified, single-chain rBoNT/F.sub.7-1 (0.201 mg/ml) was removed from the 80 C. freezer and thawed in an MSC1 cabinet. 2.8 l of Lys-C was added (0.1 mg/ml) to give a final concentration of 0.04 g/ml (1:5025 ratio). The Lys-C was initially reconstituted in dH.sub.2O to give a 0.1 mg/ml stock.

(250) The sample was incubated at 4 C. overnight (16 hours) alongside a control with no protease added. Samples were taken for SDS-PAGE analysis to make both reduced and none-reduced samples. SDS sample buffer (Invitrogen # LC2376) was used for oxidising conditions. LDS sample buffer (Invitrogen P0007) and DTT were used for reducing conditions.

Example 5Phenyl HP Polishing Step of rBoNT/F.SUB.7-1

(251) rBoNT/F.sub.7-1 was activated for 16 hours overnight after the salt concentration was adjusted to 3 M NaCl by the addition of 1.6 volumes of 50 mM Tris pH 8.0, 5 M NaCl. The sample was then loaded onto a 1 ml HiTrap Phenyl HP column which was pre-equilibrated in 50 mM Tris pH 8.0, 3 M NaCl.

(252) The column was then washed for 10 column volumes with equilibration buffer and the protein was then eluted over a 25 cv linear gradient from 3 M NaCl to 0 M salt. 1 ml fractions were taken across the gradient.

(253) 210 l samples were taken for SDS-PAGE analysis. The samples were prepared in either SDS sample buffer (none reduced) or LDS buffer+100 mM DTT (reduced) and run out on SDS-PAGE.

Example 6N-Terminal Sequencing of Activated rBoNT/F.SUB.7-1

(254) Activated rBoNT/F.sub.7-1 was subjected to N-terminal sequencing. rBoNT/F.sub.7-1 was purified, as an activated di-chain protein, as described above. Samples were prepared by adding 200 l final sample (0.1 mg/ml), to 100 l SDS loading buffer, 40 l DTT (1M), and 60 l H.sub.2O. The samples were heated for 5 min at 95 C. then loaded 1 g per lane (20 l load volume) onto gels and processed for SDS-PAGE (FIG. 8).

(255) The BoNT/F.sub.7-1 heavy chain (100 kDa band) was excised from each of 9-lanes (of a 10 lane gel) and sent to the contract research organization Alta Bioscience for N-terminal sequencing by Edman degradation. The Alta Bioscience service is accredited to ISO 17025:2005 for N-Terminal Protein Sequencing.

(256) The returned N-terminal sequence for the heavy chain of activated BoNT/F.sub.7-1 chain was:

(257) TABLE-US-00013 TABLE 5 Residue position Amino acid 1 A? 2 P 3 P 4 5 L? 6 7 I? Comments: Very weak signal just above background ? = most probable assignment = nothing detected at this position X = unknown component

(258) These results show that activated rBoNT/F.sub.7-1 is a different protein (at the level of primary sequence) compared to activated rBoNT/F.sub.7. Activated rBoNT/F.sub.7-1 contains amino acids APPR, at the extreme N-terminus of the heavy chain moiety. This amino acid sequence does not occur in native sequence rBoNT/F.sub.7.

(259) This revealed that the H.sub.N domain containing chain of the activated di-chain polypeptide comprises the N-terminal amino acid sequence APPRLCIKV (FIG. 8). Thus, this N-terminal sequence comprises the amino acid motif APPR from the BoNT/F.sub.1 activation loop, the motif LCI (which is common to both the BoNT/F.sub.1 and BoNT/F.sub.7 subtypes), and the amino acids KV from the N-terminus of the BoNT/F.sub.7 H.sub.N domain. The APPR motif (and the extended APPRLCIKV motif) are not found in native BoNT/F.sub.7 (as indicated by the circle highlighting the corresponding BoNT/F.sub.7 sequence in FIG. 8). Thus, the activated di-chain form of rBoNT/F.sub.7-1 contains a unique amino acid sequence, which is not found in either the native (unmodified) form of the BoNT/F.sub.1 or BoNT/F.sub.7 subtypes.

Example 7Activity of rBoNT/F.SUB.7-1

(260) The activity of rBoNT/F.sub.7-1 was determined using a number of standard assays, including a cell-free protease activity assay (FIG. 9), a spinal cord neuron (SCN) glycine (Gly) release inhibition assay (FIG. 10), an inhibition of ex-vivo muscle contraction assay (FIG. 11) and an in vivo DAS assay (FIG. 12).

(261) Cell-Free Protease Activity Assay

(262) The method for cell-free protease assay of rBoNT/F.sub.7-1 was as follows:

(263) A BEH300 C4 reverse phase analytical HPLC column was equilibrated in 0.1% TFA in water (buffer A) for 30 minutes at 0.7 mL/min (starting from 0.1 mL/min and gradually increasing the flow over the First few minutes). The reference and test samples were diluted to 1 nM in 100 l total volume with 0.2 mg/mL BSA, 2.0 mM DTT, 50 mM Hepes and 20 M ZnCl2 (assay buffer). The test samples and 100 l of assay buffer (for negative control) were pre-incubated for 30 minutes at 37 C. The substrate was prepared by diluting a concentrated VAMP2-GFP stock solution to 20 M with assay buffer. 50 l of each test sample (or buffer into the negative control) was added to the 50 l of substrate and mixed by pipetting gently, and then incubated at 37 C. for 30 minutes. The reactions were then stopped by adding 25 l 0.3M EDTA per tube, mixed by pipetting gently. The samples were diluted by adding 375 l of buffer A to each reaction mix. Each sample was filtered using luer lock syringe and 0.2 m PTFE filter. To run the samples, the column temperature was set to 40 C., the detection wavelength to 280 nm, the same injection to 100 l and the gradient as presented in Table 6 below:

(264) TABLE-US-00014 TABLE 6 Time (min) % Buffer A % Buffer B Event 0-2 100 0 Flow through 2.1 61 39 Gradient 8 60.2 39.8 8.1 20 80 Stripping 11 20 80 11.1 100 0 Re-equilibration 15 100 0

(265) Duplicate injections of samples were analysed over an elution period of 15 minutes at 0.7 mL/min.

(266) As shown in FIG. 9B, the rBoNT/F.sub.7-1 was able to cleave the VAMP2 substrate according to the process identified in the schematic of FIG. 9A. rBoNT/F.sub.7-1 was shown to yield a higher % of cleaved product compared with the native BoNT/F control (65% versus 46%, as well as greater specific activity (26 nmol/h/pmol vs 18 nmol/h/pmol), as shown in FIG. 9C.

(267) SCN Gly Release Inhibition Assay

(268) The method for SCN gly release inhibition assay of rBoNT/F.sub.7-1 was as follows:

(269) Primary cultures of rat spinal cord neurons (SCN) were prepared and grown, for 3 weeks, in 96 well tissue culture plates (as described in: Masuyer et al., 2011, J. Struct. Biol. Structure and activity of a functional derivative of Clostridium botulinum neurotoxin B; and in: Chaddock et al., 2002, Protein Expr. Purif. Expression and purification of catalytically active, non-toxic endopeptidase derivatives of Clostridium botulinum toxin type A). Serial dilutions of BoNT were prepared in SCN feeding medium. The growth medium from the wells to be treated was collected and filtered (0.2 m filter). 125 l of the filtered medium was added back to each test well. 125 l of diluted toxin was then added to the plate (triplicate wells). The treated cells were incubated at 37 C., 10% CO.sub.2, for 241 hours).

(270) TABLE-US-00015 TABLE 7 Example plate layout for two dose-response curves 1 2 3 4 5 6 7 8 9 10 11 12 NOTES A B Basal 0.0001 0.0003 0.001 0.003 0.01 0.03 0.1 0.3 1 Native BoNT/F C standard (nM) D E Basal 0.0001 0.0003 0.001 0.003 0.01 0.03 0.1 0.3 1 Test sample (nM) F G H

(271) This example shows example concentration ranges used for native BoNT/F supplied by List Biological laboratories (Cambell, Calif., USA), compared to a recombinant test protein. The concentrations shown are examples of concentrations that might be expected to yield a, well defined, dose-response curve for these two proteins, with data points covering both the upper and lower asymptote regions. In other cases, the actual concentrations tested, which were most optimal for any particular experiment, were first determined empirically.

(272) As shown in FIG. 10, the % glycine release of rBoNT/F.sub.7-1 was greater than that of the native BoNT/F control at the same concentration.

(273) [.sup.3H] Glycine Release Assay

(274) BoNT Treated SCN Loaded with Radio-Labelled Glycine

(275) BoNT treated SCN were removed from the incubator (241 hours after exposure to BoNT) then the medium removed. 280 l warmed HEPES-buffered MEM (12.5 ml HEPES in 500 ml MEM, 25 mM final concentration) was added to each well (first wash). The plate was then returned to the incubator for 5 min. The 280 l warmed HEPES-buffered MEM (added in the step above) was removed and replaced with a second 280 l HEPES-buffered MEM (second wash) and incubate at 35 C. for 5 minutes. The second wash solution was removed and 180 l [.sup.3H]glycine (2 Ci/ml (Perkin Elmer 1 mCi/ml NET00400), in HEPES-buffered MEM) was added to each well. The plate was then incubated for 30 minutes at 35 C. 320 l of the [.sup.3H]glycine working stock was retained for counting. After the 30 minute incubation, the [.sup.3H]glycine was removed from cells. The cells were washed once for five minutes with HEPES-buffered MEM (250 l/well), then three times with HEPES-buffered (10 mM) HBSS: 200, 210, 230 l/well in successive washes (5 minutes per wash).

(276) Collect Basal and Stimulated Secretion Samples

(277) For basal reading, 100 l/well of HBSS was applied gently to all wells. The clock was started at the point of addition to the first well. After 5 min, the contents of the wells were transferred to the pre-chilled tubes, and these samples held at 4 C. until further processing. For stimulated reading, 100 l/well of HBSS containing 56 mM KCl was gently applied to all wells. The clock was started at the point of addition to the first well. After 5 min, the contents of the wells were transferred to the pre-chilled tubes, and these samples held at 4 C. until further processing. The cells were lysed in the plate with 100 l of 2 M acetic acid/0.1% trifluoroacetic acid (lysis solution) for 10 min, pipetted up and down three times then transfer to final rack of collection tubes. The basal and stimulated samples were centrifuged in a microfuge at 13000 rpm (16.100g) for 5 minutes. 50 l of each supernatant was transferred to a 6 ml scintillation vial and 4 ml Optima Gold MV scintillation fluid added. Counting was carried out using a Perkin Elmer TriCarb 2100 liquid scintillation analyser (LSA) (or equivalent) using a programme written for tritium, using 3 min count times and giving a DPM output.

(278) Data Analysis

(279) The recorded depolarisations per minute (DPM) values were multiplied by two (since only 50 l of 100 l collected was counted in the vial). The basal DPM was calculated as % of the (basal+stimulated+lysate) DPM. The stimulated DPM was calculated as % of the (stimulated+lysate) DPM. The Net % release was calculated by subtracting the basal DPM % from the stimulated DPM %. The Net % release for each BoNT treatment was calculated as a % of the average triplicate basal control wells Net % release associated with that treatment (ie, the basal controls will equal 100%, when averaged). The triplicate Net % release values for each BoNT concentration was averaged and the triplicate Net % release as % of average control % release values for each BoNT concentration was averaged.

(280) Table 8 below provides the EC.sub.50 values determined for rBoNT/F.sub.7-1 and a control (F/080166/Metv3) in the SCN Gly inhibition assay. The control (F/080166/Metv3) is commercially available, native (not-recombinant) BoNT/F. These results show that recombinant BoNT/F.sub.7-1 is more potent than purified, naturally occurring BoNT/F, in this assay.

(281) TABLE-US-00016 TABLE 8 Protein EC.sub.50 (Log M) SXN102785 10.10 0.09 F/080116/METv3 9.36 0.11
Inhibition of Ex-Vivo Muscle Contraction Assay

(282) The method to measure the activity of BoNT/F.sub.7-1 in the inhibition of ex-vivo muscle contraction assay was as follows:

(283) Krebs-Henseleit buffer was prepared on the day of the assay: to approximately 1400 ml of dH.sub.2O, the following were added: 47 ml*5 M NaCl (Sigma S6546), 2.4 ml*1 M MgSO.sub.4 (Sigma M3409), 8.9 ml*45% .sub.D-Glucose (Sigma G8769), 100 ml*20 KCl, 100 ml*20 KH.sub.2PO.sub.4, 20 ml*250 mM CaCl.sub.2, 50 ml*1 M NaHCO.sub.3, and the pH adjusted to 7.5 as necessary The final volume was then adjusted to 2 litres, with dH.sub.2O

(284) An EmkaBath4 tissue bath system (or other suitable organ bath equipment) was set up according to the manufacturer's instructions. A left side phrenic nerve/hemi-diaphragm tissue preparation was prepared as described previously (Rasetti-Escarguil et al., 2009, Toxicon, Measurement of botulinum types A, B, and E neurotoxicity using the pherenic nerve-hemidiaphragm: improved precision with in-bred mice; and in Rasetti-Escarguil et al., 2011, Toxicon, Pherenic nerve-hemidiaphragm as a highly sensitive replacement assay for determination of functional botulinum toxin antibodies). The phrenic nerve/hemi-diaphragm was attached to the tissue holder/electrode assembly in the tissue bath. The transducer was then moved up/down to set the tension to approximately 1 g. The tension in the tissue was monitored and small adjustments made as necessary to keep it close to 1 g during an initial baseline stabilisation phase. A tubocurarine control was performed by adding 100 l of 100 tubocurarine directly to the bath. When full paralysis of the tissue (caused by the tubocurarine) registered on the trace, the tubocurarine was washed out and the baseline contraction allowed to become re-established. The BoNT to be tested was diluted to 10 the desired final concentration, in buffer containing 0.05% gelatine. 1 ml buffer was removed from the bath and 1 ml of 10 BoNT added directly to the bath, which was then mixed by pipetting up/down 5 times. The tension in the tissue was monitored and the post-toxin force calculated as a percentage of the mean pre-toxin force and plotted against time.

(285) The data was then fitted to a modification of the Hill equation (Equation 1)

(286) $\begin{array}{cc}y=\mathrm{bottom}+\frac{\left(\mathrm{top}-\mathrm{bottom}\right)}{1+{10}^{{\left(t_{50}-x\right)}^{n_H}}}& \mathrm{Equation}\left(1\right)\end{array}$

(287) Where x is the time (min), y is the force and t.sub.50 is the time to reduce the contraction to 50% of the pre-toxin level. Data from this assay are rejected if: the pre-treatment developed force is less than 0.5 g; the response to 3 M D-tubocurarine is <94% inhibition; and/or a short burst of direct muscle stimulation at the end of the experiment does not result in muscle contraction.

(288) As shown in FIG. 11 (left-hand graph), rBoNT/F.sub.7-1 was able to completely inhibit muscle contraction by 80 minutes post-treatment. FIG. 11 (right-hand graph) shows the t.sub.50 values for rBoNT/F.sub.7-1, rBoNT/F.sub.1 and a control (F/080166/Metv3), and demonstrates that rBoNT/F.sub.7-1 has a shorter t.sub.50 than either rBoNT/F.sub.1 or the control. Table 9 below provides the t.sub.50 values for rBoNT/F.sub.7-1, rBoNT/F.sub.1 and a control (F/080166/Metv3) in the ex vivo muscle contraction assay shown in FIG. 11. The control (F/080166/Metv3) is commercially available, native (not-recombinant) BoNT/F. These results show that recombinant BoNT/F.sub.7-1 is more potent than (lower t.sub.50 value=more potent) purified, naturally occurring BoNT/F and recombinant BoNT/F.sub.1, in this assay.

(289) TABLE-US-00017 TABLE 9 Protein Concentration (pM) t.sub.50 (min) SXN102785 (rBoNT/F.sub.7-1) 100 44 1 SXN102691 (rBoNT/F.sub.1) 100 55 1 F/080116/METv3 100 59 2
Digit Abduction Scoring (DAS) Assay

(290) The method to measure the activity of BoNT/F7-1 in the DAS assay was as follows:

(291) The method is based on the startled response toe spreading reflex of mice, when suspended briefly by the tail. This reflex is scored as Digit Abduction Score (DAS) and is inhibited after administration of BoNT into the gastrocnemius-soleus muscles of the hind paw. Mice are suspended briefly by the tail to elicit a characteristic startled response in which the animal extends its hind limb and abducts its hind digits. (Aoki et al. 1999, Eur. J. Neurol.; 6 (suppl. 4) S3-S10)

(292) On the day of injection, mice are anaesthetized in an induction chamber receiving isoflurane 3% in oxygen. Each mouse receives an intramuscular injection of BoNT or vehicle (phosphate buffer containing 0.2% gelatine) in the gastrocnemius-soleus muscles of the right hind paw.

(293) Following neurotoxin injection, the varying degrees of digit abduction are scored on a scale from zero to four:

(294) Where 0=normal and 4=maximal reduction in digit abduction and leg extension. ED50 is determined by nonlinear adjustment analysis using average of maximal effect at each dose. The mathematical model used is the 4 parameters logistic model.

(295) DAS is performed every 2 hours during the first day after dosing; thereafter it is performed 3 times a day for 4 days. FIG. 12 shows the fitted curves for nBoNT/F.sub.1 (used as reference), rBoNT/F.sub.1 and rBoNT/F.sub.7-1. While nBoNT/F.sub.1 and rBoNT/F.sub.1 show very similar dose response, the rBoNT/F.sub.7-1 curve is shifted to the left, meaning lower doses of rBoNT/F.sub.7-1 achieved a similar DAS response compared to nBoNT/F.sub.1 and rBoNT/F.sub.1 and therefore showing that rBoNT/F.sub.7-1 is more potent than the others in the mouse DAS assay; see also the table below (table 10) that provides the values for the calculated ED50 and the dose leading to DAS 4 (highest score) for each BoNT: to achieve a similar DAS response of 2 or 4, the dose needed is much lower for BoNT/F.sub.7-1 than rBoNT/F.sub.1 and nBoNT/F.sub.1.

(296) TABLE-US-00018 TABLE 10 ED50 DAS 4 dose protein (pg/mouse) (pg/mouse) F/053112-01/MET 12.1 [9.75-14.44] 90 rBoNT/F1 (SXN1029691) 11.7 [9.68-13.69] 90 rBoNT/F7-1 (SXN102931) 1.6 [1.41-1.83] 22.5
Sequence Information

(297) TABLE-US-00019 BoNT/Fconsensussequence SEQIDNO:1 MPVVINSFNYNDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERN 50 TIGTKPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINS 100 NPAGKVLLXEISXAKPYLGNDHTPINEFXPVNRTTSVNIKLSTNVESSML 150 LNLLVLGAGPDIFEXSCYPVRKXPNSDXVYDPSNXGFGSINIVTFSPEYE 200 YTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGAXGVTYKETIEV 250 KQXPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATR 300 LSEVNSAPPEYDINEYKDYFQWKYGLDKNADGSYTVNENKFNEIYKKLYS 350 FTEIDLANKFKVKCRNTYFIK-YGFLKVPNLLDDDIYTVSEGFNIGNLAV 400 NNRGQNINLNPKIIDSIPDKGLVEKIVKFCKSIIPRKGTKAPPRLCIRVN 450 NRELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETI 500 P0ISNRTLNTLVQDDSYVPRYDSNGTSEIEEYDVVDLNVFFYLHAQKVPE 550 GETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVI 600 RDFTTEATQKSTVDKIADISLIVPYVGLALNIGNEAEKGNFKEAFELLGA 650 GILLEFVPELXIPVILVFTIKSYIDSYENKNKIIKAINNSLIEREAKWKE 700 IYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDE 750 KNRLESEYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAKVG 800 KLKEYDKHVKSXLLNYILXHRSILGEQ-XQELNDLVTSTLNSSIPFELSS 850 YTNDKILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVY 900 IYSTNRNQFGIYSSRLSEVNIAQNNDIIYNSRYQNFSISFWVRIPKHYNP 950 MNLNREYTIINCMGNNNSGWKISLRXNXDCEIIWTLQDTSGNKEKLIFRY 1000 XQLISISDYINKWIFVTITNNRLGNSRIYINGNLIVEKSISNLGDIHVSD 1050 NILFKIVGCDDTXYVGIRYFKVFNTELDKTEIETLYSNEPDPSILKDYWG 1100 NYLLYNKKYYLLNLLRKDKYITXNSGILNINQQRGVTEX-XIFSNYKLYE 1150 GVEVIIRKNGPIDISNTDNFVRKNDLAYINVVDRGVEYRLYADTSIXKPE 1200 KTIKLIRTSNSNDSLGQIIVMDSIGNNCTMNFQNNNGSNIGLLGFHSNNL 1250 VASSWYYNNIRRNTSSNGCFWSFISKEHGWQE- 1283 BoNT/F.sub.1-UniParcAccessionNo.UPI00000B66D1 SEQIDNO:2 MPVVINSFNYNDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERN 50 TIGTDPSDFDPPASLENGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINS 100 NPAGEVLLQEISYAKPYLGNEHTPINEFHPVTRTTSVNIKSSTNVKSSII 150 LNLLVLGAGPDIFENSSYPVRKLMDSGGVYDPSNDGFGSINIVTFSPEYE 200 YTFNDISGGYNSSTESFIADPAISLAHELIHALHGLYGARGVTYKETIKV 250 KQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATR 300 LSRVNSAPPEYDINEYKDYFQWKYGLDKNADGSYTVNENKFNEIYKKLYS 350 FTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNIGNLAVN 400 NRGQNIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNN 450 RELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETIP 500 QISNQTLNTLVQDDSYVPRYDSNGTSEIEEHNVVDLNVFFYLHAQKVPEG 550 ETNISLTSSIDTALSEESQVYTFFSSEFINTINKPVHAALFISWINQVIR 600 DFTTEATQKSTFDKIADISLVVPYVGLALNIGNEVQKENFKEAFELLGAG 650 ILLEFVPELLIPTILVFTIKSFIGSSENKNKIIKAINNSLMERETKWKEI 700 YSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNNYTSDER 750 NRLESEYNINNIREELNKKVSLAMENIERFITESSIFYLMKLINEAKVSK 800 LREYDEGVKEYLLDYISEHRSILGNSVQELNDLVTSTLNNSIPFELSSYT 850 NDKILILYFNKLYKKIKDNSILDMRYENNKFIDISGYGSNISINGDVYIY 900 STNRNQFGIYSSKPSEVNIAQNNDIIYNGRYQNFSISFWVRIPKYFNKVN 950 LNNEYTIIDCIRNNNSGWKISLNYNKIIWTLQDTAGNNQKLVFNYTQMIS 1000 ISDYINKWIFVTITNNRLGNSRIYINGNLIDEKSISNLGDIHVSDNILFK 1050 IVGCNDTRYVGIRYFKVFDTELGKTEIETLYSDEPDPSILKDFWGNYLLY 1100 NKRYYLLNLLRTDKSITQNSNFLNINQQRGVYQKPNIFSNTRLYTGVEVI 1150 IRKNGSTDISNTDNFVRKNDLAYINVVDRDVEYRLYADISIAKPEKIIKL 1200 IRTSNSNNSLGQIIVMDSIGNNCTMNFQNNNGGNIGLLGFHSNNLVASSW 1250 YYNNIRKNTSSNGCFWSFISKEHGWQEN 1278 BoNT/F.sub.2-UniParcAccessionNo.UPI00016BB5D7 SEQIDNO:3 MPVVINSFNYNDPVNDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERD 50 TIGTKPDEFQVPDSLKNGSSAYYDPNYLTTDAEKDRYLKTMIKLFNRINS 100 NPTGKVLLEEVSNARPYLGDDDTLINEFFPVNVTTSVNIKFSTDVESSII 150 SNLLVLGAGPDIFKAYCTPLVRFNKSDKLIEPSNHGFGSINILTFSPEYE 200 HIFNDISGGNHNSTESFIADPAISLAHELIHALHGLYGAKAVTHKESLVA 250 ERGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATR 300 LREVNTAPPGYDINEYKDYFQWKYGLDRNADGSYTVNRNKFNEIYKKLYS 350 FTEIDLANKFKVKCRNTYFIKYGFVKVPDLLDDDIYTVSEGFNIGNLAVN 400 NRGQNINLNPKIIDSIPDKGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNN 450 RELFFVASESSYNESDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETIP 500 QISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQKVPEG 550 ETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIR 600 DFTTEATQKSTVDKIADISLIVPYVGLALNIVIEAEKGNFEEAFELLGAG 650 ILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWKEI 700 YSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEK 750 NRLESKYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAEVGK 800 LKEYDKHVKSDLLDYILYHKLILGEQTKELIDLVTSTLNSSIPFELSSYT 850 NDKILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIY 900 STNRNQFGIYSGRLSEVNIAQNNDIIYNSRYONFSISFWVRIPKHYRPMN 950 RNREYTIINCMGNNNSGWKISLRTIRDCEIIWTLQDTSGNKEKLIFRYEE 1000 LASISDYINKWIFVTITNNRLGNSRIYINGNLIVEKSISNLGDIHVSDNI 1050 LFKIVGCDDETYVGIRYFKVFNTELDKTEIETLYSNEPDPSILKDYWGNY 1100 LLYNKKYYLFNLLRKDKYITRNSGILNINQQRGVTGGISVFLNYKLYEGV 1150 EVIIRKNAPIDISNTDNFVRKNDLAYINVVDHGVEYRLYADISITKSEKI 1200 IKLIRTSNPNDSLGQIIVMDSIGNNCTMNFQNNDGSNIGLLGFHSDDLVA 1250 SSWYYNHIRRNTSSNGCFWSFISKEHGWKE 1280 BoNT/F.sub.2-UniParcAccessionNo.UPI00000B50F1 SEQIDNO:4 MPVVINSFNYNDPVNDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERD 50 TIGTKPDEFQVPDSLKNGSSAYYDPNYLTTDAEKDRYLKTMIKLFNRINS 100 NPTGKVLLEEVSNARPYLGDDDTLINEFLPVNVTTSVNIKFSTDVESSII 150 SNLLVLGAGPDIFKAYCTPLVRFNKSDKLIEPSNHGFGSINILTFSPEYE 200 HIFNDISGGNHNSTESFIADPAISLAHELIHALHGLYGAKAVTHKESLVA 250 ERGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATR 300 LREVNTAPPGYDINEYKDYFQWKYGLDRNADGSYTVNRNKFNEIYKKLYS 350 FTEIDLANKFKVKCRNTYFIKYGFVKVPNLLDDDIYTVSEGFNIGNLAVN 400 NRGQNINLNPKIIDSIPDKGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNN 450 RELFFVASESSYNESDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETIP 500 QISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQKVPEG 550 ETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIR 600 DFTTEATQKSTVDKIADISLIVPYVGLALNIVIEAEKGNFEEAFELLGAG 650 ILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWKEI 700 YSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEK 750 NRLESKYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAEVGK 800 LKEYDKHVKSDLLDYILYHKLILGEQTKELIDLVTSTLNSSIPFELSSYT 850 NDKILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIY 900 STNRNQFGIYSGRLSEVNIAQNNDIIYNSRYQNFSISFWVTIPKHYRPMN 950 RNREYTIINCMGNNNSGWKISLRTIRDCEIIWTLQDTSGNKEKLIFRYEE 1000 LASISDYINKWIFVTITNNRLGNSRIYINGNLIVEKSISNLGDIHVSDNI 1050 LFKIVGCDDETYVGIRYFKVFNTELDKTEIETLYSNEPDPSILKDYWGNY 1100 LLYNKKYYLFNLLRKDKYITRNSGILNINQQRGVTGGISVFLNYKLYEGV 1150 EVIIRKNAPIDISNTDNFVRKNDLAYINVVDHGVEYRLYADISITKSEKI 1200 IKLIRTSNPNDSLGQIIVMDSIGNNCTMNFQNNDGSNIGLLGFHSDDLVA 1250 SSWYYNHIRRNTSSNGCFWSFISKEHGWKE 1280 BoNT/F.sub.3-UniParcAccessionNo.UPI0001C0B12F SEQIDNO:5 MPVVINSFNYNDPVNDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERD 50 TIGTKPDDFQVPDSLKNGSSAYYDPNYLTTDAEKDRYLKTMIKLFNRINS 100 NPTGKVLLEEVSNARPYLGDDDTLINEFFPVNVTTSVNIKFSTDVESSII 150 SNLLVLGAGPDIFKAYCTPLVRFNKSDKLIEPSNHGFGSINILTFSPEYE 200 HIFNDISGGDHNSTESFIADPAISLAHELIHALHGLYGAKAVTHKETIEV 250 KRGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATR 300 LREVNTAPPEYDINEYKDYFQWKYGLDRNADGSYTVNRNKFNGIYKKLYS 350 FTEIDLANKFKVKCRNTYFIKYGFVKVPDLLDDDIYTVSEGFNIGNLAVN 400 NRGQNINLNPKIIDSIPDKGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNN 450 RELFFVASESSYNESDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETIP 500 QISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQKVPEG 550 ETNISLTSSIDTALLEKSKVYTFFSSEFIDTINESVNAALFIDWINKVIR 600 DFTTEATQKSTVDKIADISLIVPYVGLALNIVIDAEKGNFQEAFELLGAG 650 ILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNALIEREAKWKEI 700 YSWIVSNWLTKINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEK 750 NRLESEYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAEVGK 800 LKKYDRHVKSDLLDYILYHKLILGDQTKELIDLVTSTLNSSIPFELSSYT 850 NDKILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIY 900 STNRNQFGIYSDRLSEVNIAQNNDIIYNSRYQNFSISFWVRIPKHYGPMN 950 RNREYTIINCMGNNNSGWKISLRNIRDCEIIWTLQDTSGNKEKLIFRYEE 1000 LANISDYINKWIFVTITNNRLGNSRIYINGNLIVEKSISNLGDIHVSDNI 1050 LFKIVGCDDKTYVGIRYFKVFNTELDKTEIETLYSNEPDPSILKDYWGNY 1100 LLYNKKYYLFNLLRKDKYITRNSGILNINQQRGVTEGSVFLNYKLYEGVE 1150 VIIRKNGPIDISNTDNFVRKNDLAYINVVYHDVEYRLYADISITKPEKII 1200 KLIRTSNPNDSLGQIIVMDSIGNNCTMNFQNNNGGNIGLLGFHSDNLVAS 1250 SWYYNNIRRNTSSNGCFWSFISKEHGWQE 1279 BoNT/F.sub.4-UniParcAccessionNo.UPI0001A92658 SEQIDNO:6 MPVVINSFNYDDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVWIMPERN 50 TIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINS 100 NPAGEVLLQEISYAKPYLGNDHTPINEFHPVTRTTSVNIKSSTNVESSII 150 LNLLVLGAGPNIFENSSYPVRKLMNSGEVYDPSNDGFGSINIVTFSPEYE 200 YTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGVTYKETIKV 250 KQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNDLLANYEKIATR 300 LSEVNSAPPEYDINEYKNYFQWKYGLDKNADGSYTVNENKFNEIYKKLYS 350 FTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNIGNLAVN 400 NRGQNINLNPKIIDSIPDKGLVEKIVKLCKSIIPRKGTKAPPRLCIRVNN 450 RELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETIP 500 QISSQTLNTLVQDDSYVPRYDSNGTSEIEEHNVVDLNAFFYLHAQKVPEG 550 ETNISLTSSIDTALSEESKVYTFFSSEFINNINKPVHAALFIGWISQVIR 600 DFTTESTQKSTVDKIADISLIVPYVGLALNIGNDARKGNFKEAFELLGAA 650 ILLEVVPELLIPVILVFTIKSFIDSSKNEDKIIKAINNSLIEREAKWKEV 700 YSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNSYTSDEK 750 NRLESEYNINNIEEELNKKVSLAMKNIERFIAESSISYLMKLINEAKVSE 800 LREYDEGVKEYLLDYILKNGSILGDHVQELNDLVTSTLNSSIPFELSSYT 850 NDKILIIYFNKLYKKIKDNCILDMRYENNKFIDISGYGSNISINGELYIY 900 TTNRNQFTIYSGKLSEVNIAQNNDIIYNSRYQNFSISFWVRIPRYSNIVN 950 LNNEYTIINCMGNNNSGWKISLNYNKIIWTLQDTAGNNEKLVFNYTQMIS 1000 ISDYINKWIFVTITNNRLGNSRIYINGNLIDQKSISNLGDIHVSDNILFK 1050 IVGCNDTRYVGIRYFKVFDTELDKTEIETLYSDEPDPSILKDFWGNYLLY 1100 NKRYYLLNLLRKDKSITQSSTFLSISRPRGVDRKANIFSNKRLYKGVEVI 1150 IRKNEPIDISNTDNFVRKGDLAYINVVDRDVEYRLYADTSNAQPEKTIKL 1200 IRTSNPDDSLGQIIVMDSIGNNCTMNFQNNNGGNIGLLGFHSNTLVASSW 1250 YYNNIRRNTSSNGCFWSFISKEHGWQEN 1278 BoNT/F.sub.4-UniParcAccessionNo.UPI0001C0812D SEQIDNO:7 MPVVINSFNYDDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVWIMPERN 50 TIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINS 100 NPAGEVLLQEISYAKPYLGNDHTPINEFHPVTRTTSVNIKSSTNVESSII 150 LNLLVLGAGPNIFENSSYPVRKLMNSGEVYDPSNDGFGSINIVTFSPEYE 200 YTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGVTYKETIKV 250 KQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNDLLANYEKIATR 300 LSEVNSAPPEYDINEYKNYFQWKYGLDKNADGSYTVNENKFNEIYKKLYS 350 FTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNIGNLAVN 400 NRGQNINLNPKIIDSIPDKGLVEKIVKLCKSIIPRKGTKAPPRLCIRVNN 450 RELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETIP 500 QISSQTLNTLVQDDSYVPRYDSNGTSEIEEHNVVDLNAFFYLHAQKVPEG 550 ETNISLTSSIDTALSEESKVYTFFSSEFINNINKPVHAALFIGWISQVIR 600 DFTTESTQKSTVDKIADISLIVPYVGLALNIGNDARKGNFKEAFELLGAA 650 ILLEVVPELLIPVILVFTIKSFIDSSKNEDKIIKAINNSLIEREAKWKEV 700 YSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNSYTSDEK 750 NRLESEYNINNIEEELNKKVSLAMKNIERFIAESSISYLMKLINEAKVSE 800 LREYDEGVKEYLLDYILKNGSILGDHVQELNDLVTSTLNSSIPFELSSYT 850 NDKILIIYFNKLYKKIKDNCILDMRYENNKFIDISGYGSNISINGELYIY 900 TTNRNQFTIYSGKLSEVNIAQNNDIIYNSRYQNFSISFWVRIPRYSNIVN 950 LNNEYTIINCMGNNNSGWKISLNYNKIIWTLQDTAGNNEKLVFNYTQMIS 1000 ISDYINKWIFVTITNNRLGNSRIYINGNLIDQKSISNLGDIHVSDNILFK 1050 IVGCNDTRYVGIRYFKVFDTELDKTEIETLYSDEPDPSILKDFWGNYLLY 1100 NKRYYLLNLLRKDNAITQSSTFLSISRARGVDRKANIFSNKRLYKGVEVI 1150 IRKNEPIDISNTDNFVRKGDLAYINVVDRDVEYRLYANTSNAQPEKTIKL 1200 IRTSNSNDSLDQIIVMDSIGNNCTMNFQNNNGGNIGLLGFHSNTLVASSW 1250 YYNNIRRNTSSNGCFWSFISKEHGWQE 1277 BoNT/F.sub.5-UniParcAccessionNo.UPI0001C0B12E SEQIDNO:8 MPVEINSFNYDDLVNDNTILYIRPPYYERSNTYFKAFNIMENVWIIPERY 50 RLGIEASKFDPPDSLKAGSDGYFDPNYLSTNTEKNRYLQIMIKLFKRINS 100 MEAGKILLMQIKDAIPYLGNSYTAEDQFTTNNRTISFMVRLANGTIEQEM 150 ANLIIWGPGPDLTTNRTGGTTYTPAQSLEAIPYKEGFGSIMTIEFSPEYA 200 TAFNDISLTSHAPSLFIKDPALILMHELIHVLHGLYGTYTTGFKIKPNIT 250 EPYMEVTKPITSGEFLTFGGNDVNKIPQLIQSQLRSKVLDDYEKIASRLN 300 KVNRATAEINIDKFKYSYQLKYQFVKDSNGVYSVDLDKFNKLYDKIYSFT 350 EFNLAHEFKIKTRNSYLAKNFGPFYLPNLLDNSIYNEADGFNIGDLSVNY 400 KGQVIGSDIDSIKKLEGQGVVSRVVRLCLNSSFKKNTKKPLCITVNNGDL 450 FFIASEDSYGEDTINTPKEIDDTTTLVPSFKNILDKVILDFNKQVTPQIP 500 NRRIRTDIQEDNYIPEYDSNGTSEIEEYNVVDLNAFFYLHAQKVPEGETN 550 ISLTSSIDTALSEESKVYTFFSSEFIDTINEPVNAALFIDWISKVIRDFT 600 TEATQKSTVDKIADISLIVPYVGLALNIVNETEKGNFKEAFELLGAGILL 650 EFVPELAIPVILVFTIKSYIDSYENKNKIIKAINNSLIEREAKWKEIYSW 700 IVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRL 750 ESEYNINNIEEELNKKVSLAMKNIERFITESSISYLMKLINEAEVGKLKE 800 YDKRVKRHLLEYIFDYRLILGEQGGELIDLVTSTLNTSIPFELSSYTNDK 850 ILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIYSTN 900 RNQFGIYDDRLSEVNIAQNNDIIYNSRYQNFSISFWVRIPKHYRPMNHNR 950 EYTIINCMGNNNSGWKISLRTTGDCEIIWTLQDTSGNKKKLIFRYSQLGG 1000 ISDYINKWIFVTITNNRLGNSRIYINGNLIVEKSISNLGDIHVSDNILFK 1050 IVGCDDKMYVGIRYFKVFNTELDKTEIEILYSNEPDPSILKDYWGNYLLY 1100 NKKYYLLNLLRNDKYITRNSDILNISHQRGVTKDLFIFSNYKLYEGVEVI 1150 IRKNGPIDISNTDNFVRKNDLAYINVVDHGVEYRLYADISITKPEKIIKL 1200 IRRSNPDDSLGQIIVMDSIGNNCTMNFQNNNGGNIGLLGFHSDNLVASSW 1250 YYNNIRRNTSSNGCFWSFISKEHGWQE 1277 BoNT/F.sub.6-UniParcAccessionNo.UPI0001C0B1D1 SEQIDNO:9 MPVAINSFNYNDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERN 50 TIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINS 100 NPAGKVLLQEISYAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSML 150 LNLLVLGAGPDIFESCCYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYE 200 YTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGVTYEETIEV 250 KQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATR 300 LSEVNSAPPEYDINEYKDYFQWKYGLDKNADGSYTVNENKFNEIYKKLYS 350 FTESDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNIGNLAVN 400 NRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNN 450 SELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSQTIP 500 QISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQKVPEG 550 ETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIR 600 DFTTEATQKSTVDKIADISLIVPYVGLALNIIIEAEKGNFEEAFELLGVG 650 ILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWKEI 700 YSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEK 750 NRLESEYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAKVGK 800 LKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSSYT 850 NDKILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIY 900 STNRNQFGIYNSRLSEVNIAQNNDIIYNSRYNFFSISFWVRIPKHYKPMN 950 HNREYTIIMCMGNNNSGWKISLRTVRDCEIIWTLQDTSGNKENLIFRYEE 1000 LNRISNYINKWIFVTITNNRLGNSRIYINGNLIVEKSISNLGDIHVSDNI 1050 LFKIVGCDDETYVGIRYFKVFNTELDKTEIETLYSNEPDPSILKDYWGNY 1100 LLYNKKYYLFNLLRKDKYITLNSGILNINQQRGVTEGSVFLNYKLYEGVE 1150 VIIRKNGPIDISNTDNFVRKNDLAYINVVDRGVEYRLYADTKSEKEKIIR 1200 TSNLNDSLGQIIVMDSIGNNCTMNFQNNNGSNIGLLGFHSNNLVASSWYY 1250 NNIRRNTSSNGCFWSFISKENGWKE 1275 BoNT/F.sub.6-UniParcAccessionNo.UPI0000126B8A SEQIDNO:10 MPVAINSFNYNDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERN 50 TIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINS 100 NPAGKVLLQEISYAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSML 150 LNLLVLGAGPDIFESCCYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYE 200 YTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGVTYEETIEV 250 KQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATR 300 LSEVNSAPPEYDINEYKDYFQWKYGLDKNADGSYTVNENKFNEIYKKLYS 350 FTESDLANKFKVKCRNTYFIKYEFLKVPNLLDDDIYTVSEGFNIGNLAVN 400 NRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNN 450 SELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSQTIP 500 QISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQKVPEG 550 ETNISLTSSIDTALLEESKDIFFSSEFIDTINKPVNAALFIDWISKVIRD 600 FTTEATQKSTVDKIADISLIVPYVGLALNIIIEAEKGNFEEAFELLGVGI 650 LLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWKEIY 700 SWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKN 750 RLESEYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAKVGKL 800 KKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSSYTN 850 DKILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIYS 900 TNRNQFGIYNSRLSEVNIAQNNDIIYNSRYQNFSISFWVRIPKHYKPMNH 950 NREYTIINCMGNNNSGWKISLRTVRDCEIIWTLQDTSGNKENLIFRYEEL 1000 NRISNYINKWIFVTITNNRLGNSRIYINGNLIVEKSISNLGDIHVSDNIL 1050 FKIVGCDDETYVGIRYFKVFNTELDKTEIETLYSNEPDPSILKNYWGNYL 1100 LYNKKYYLFNLLRKDKYITLNSGILNINQQRGVTEGSVFLNYKLYEGVEV 1150 IIRKNGPIDISNTDNFVRKNDLAYINVVDRGVEYRLYADTKSEKEKIIRT 1200 SNLNDSLGQIIVMDSIGNNCTMNFQNNNGSNIGLLGFHSNNLVASSWYYN 1250 NIRRNTSSNGCFWSSISKENGWKE 1274 BoNT/F.sub.6-UniParcAccessionNo.UPI0001C0B1D0 SEQIDNO:11 MPVAINSFNYNDPVNDDTILYMQIPYKEKSKKYYKAFEIMRNVWIIPERN 50 TIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINS 100 NPAGKVLLQEISYAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSML 150 LNLLVLGAGPDIFESCCYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYE 200 YTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGVTYEETIEV 250 KQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATR 300 LSEVNSAPPEYDINEYKDYFQWKYGLDKNADGSYTVNENKFNEIYKKLYS 350 FTESDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNIGNLAVN 400 NRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNN 450 SELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSQTIP 500 QISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQKVPEG 550 ETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIR 600 DFTTEATQKSTVDKIADISLIVPYVGLALNIIIEAEKGNFEEAFELLGVG 650 ILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWKEI 700 YSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEK 750 NRLESEYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAKVGK 800 LKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSSYT 850 NDKILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIY 900 STNRNQFGIYNSRLSEVNIAQNNDIIYNSRYQNFSISFWVRIPKHYKPMN 950 HNREYTIINCMGNNNSGWKISLRTVRDCEIIWTLQDTSGNKENLIFRYEE 1000 LNRISNYINKWIFVTITNNRLGNSRIYINGNLIVEKSISNLGDIHVSDNI 1050 LFKIVGCDDETYVGIRYFKVFNTELDKTEIETLYSNEPDPSILKDYWGNY 1100 LLYNKKYYLFNLLRKDKYITLNSGILNINQQRGVTEGSVFLNYKLYEGVE 1150 VIIRKNGPIDISNTDNFVRKNDLAYINVVDRGVEYRLYADTKSEKEKIIR 1200 TSNLNDSLGQIIVMDSIGNNCTMNFQNNNGSNIGLLGFHSNNLVASSWYY 1250 NNIRRNTSSNGCFWSFISKENGWKE 1275 BoNT/F.sub.6-UniParcAccessionNo.UPI0004E7C8CE SEQIDNO:12 MPVAINSENYNDPVNDDTILYMQIPYKEKSKKYYKAFEIMRNVWIIPERN 50 TIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINS 100 NPAGKVLLQEISYAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSML 150 LNLLVLGAGPDIFESCCYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYE 200 YTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGVTYEETIEV 250 KQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATR 300 LSEVNSAPPEYDINEYKDYFQWKYGLDKNADGSYTVNENKFNEIYKKLYS 350 FTESDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNIGNLAVN 400 NRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNN 450 SELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSQTIP 500 QISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQKVPEG 550 ETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIR 600 DFTTEATQKSTVDKIADISLIVPYVGLALNIIIEAEKGNFEEAFELLGVG 650 ILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWKEI 700 YSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEK 750 NRLESEYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAKVGK 800 LKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSSYT 850 NDKILIIYFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIY 900 STNRNQFGIYNSRLSEVNIAQNNDIIYNSRYQNFSISFWVRIPKHYKPMN 950 HNREYTIINCMGNNNSGWKISLRTVRDCEIIWTLQDTSGNKENLIFRYEE 1000 LNRISNYINKWIFVTITNNRLGNSKIYINGNLIVEKSISNLGDIHVSDNI 1050 LFKIVGCDDETYVGIRYFKVFNTELDKTEIETLYSNEPDPSILKDYWGNY 1100 LLYNKKYYLFNLLRKDKYITLNSGILNINQQRGVTEGSVFLNYKLYEGVE 1150 VIIRKNGPIDISNTDNFVRKNDLAYINVVDRGVEYRLYADTKSEKEKIIR 1200 TSNLNDSLGQIIVMDSIGNNCTMNFQNNNGSNIGLLGFHSNNLVASSWYY 1250 NNIRRNTSSNGCFWSFISKENGWKE 1275 BoNT/F.sub.7-UniParcAccessionNo.UPI0001DE3DAC SEQIDNO:13 MPVNINNFNYNDPINNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERN 50 IIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKTVIKLFNRINS 100 NPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESKGTTDSML 150 LNLVILGPGPNILECSTFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEY 200 AFNDNTDLFIADPAISLAHELIHVLHGLYGAKGVTNKKVIEVDQGALMAA 250 EKDIKIEEFITFGGQDLNIITNSTNQKIYDNLLSNYTAIASRLSQVNINN 300 SALNTTYYKNFFQWKYGLDQDSNGNYTVNISKFNAIYKKLFSFTECDLAQ 350 KFQVKNRSNYLFHFKPFRLLDLLDDNIYSISEGFNIGSLRVNNNGQNINL 400 NSRIVGPIPDNGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASES 450 SYNENGINSPKEIDDTTITNNNYKKNLDEVILDYNSDAIPNLSSRLLNTT 500 AQNDSYVPKYDSNGTSEIKEYTVDKLNVFFYLYAQKAPEGESAISLTSSV 550 NTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKS 600 TIDKIADISLVVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELL 650 IPTILVFTIKSFINSDDSKNKIIKAINNALRERELKWKEVYSWIVSNWLT 700 RINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIY 750 SIKEELNKKVSLAMQNIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQ 800 YLLNYILENSSTLGTSSVQELNNLVSNTLNNSIPFELSEYTNDKILISYF 850 NRFYKRIIDSSILNMKYENNRFIDSSGYGSNISINGDIYIYSTNRNQFGI 900 YSSRLSEVNITQNNTIIYNSRYQNFSVSFWVRIPKYNNLKNLNNEYTIIN 950 CMRNNNSGWKISLNYNNIIWTLQDTTGNNQKLVFNYTQMIDISDYINKWT 1000 FVTITNNRLGHSKLYINGNLTDQKSILNLGNIHVDDNILFKIVGCNDTRY 1050 VGIRYFKIFNMELDKTEIETLYHSEPDSTILKDFWGNYLLYNKKYYLLNL 1100 LKPNMSVTKNSDILNINRQRGIYSKTNIFSNARLYTGVEVIIRKVGSTDT 1150 SNTDNFVRKNDTVYINVVDGNSEYQLYADVSTSAVEKTIKLRRISNSNYN 1200 SNQMIIMDSIGDNCTMNFKTNNGNDIGLLGFHLNNLVASSWYYKNIRNNT 1250 RNNGCFWSFISKEHGWQE 1268 BoNT/F.sub.7-UniParcAccessionNo.UPI0001C0E220 SEQIDNO:14 MPVNINNFNYNDPINNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERN 50 IIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKTVIKLFNRINS 100 NPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESNGTTDSML 150 LNLVILGPGPNILECSTFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEY 200 AFNDNTDLFIADPAISLAHELIHVLHGLYGAKGVTNKKVIEVDQGALMAA 250 EKDIKIEEFITFGGQDLNIITNSTNQKIYDNLLSNYTAIASRLSQVNINN 300 SALNTTYYKNFFQWKYGLDQDSNGNYTVNISKFNAIYKKLFSFTECDLAQ 350 KFQVKNRSNYLFHFKPFRLLDLLDDNIYSISEGFNIGSLRVNNNGQNINL 400 NSRIVGPIPDNGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASES 450 SYNENGINSPKEIDDTTITNNNYKKNLDEVILDYNSDAIPNLSSRLLNTT 500 AQNDSYVPKYDSNGTSEIKEYTVDKLNVFFYLYAQKAPEGESAISLTSSV 550 NTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKS 600 TIDKIADISLVVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELL 650 IPTILVFTIKSFINSDDSKNKIIKAINNALRERELKWKEVYSWIVSNWLT 700 RINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIY 750 SIKEELNKKVSLAMQNIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQ 800 YLLNYILENSSTLGTSSVQELNNLVSNTLNNSIPFELSEYTNDKILISYF 850 NRFYKRIIDSSILNMKYENNRFIDSSGYGSNISINGDIYIYSTNRNQFGI 900 YSSRLSEVNITQNNTIIYNSRYQNFSVSFWVRIPKYNNLKNLNNEYTIIN 950 CMRNNNSGWKISLNYNNIIWTLQDTTGNNQKLVFNYTQMIDISDYINKWT 1000 FVTITNNRLGHSKLYINGNLTDQKSILNLGNIHVDDNILFKIVGCNDTRY 1050 VGIRYFKIFNMELDKTEIETLYHSEPDSTILKDFWGNYLLYNKKYYLLNL 1100 LKPNMSVTKNSDILNINRQRGIYSKTNIFSNARLYTGVEVIIRKVGSTDT 1150 SNTDNFVRKNDTVYINVVDGNSEYQLYADVSTSAVEKTIKLRRISNSNYN 1200 SNQMIIMDSIGDNCTMNFKTNNGNDIGLLGFHLNNLVASSWYYKNIRNNT 1250 RNNGCFWSFISKEHGWQE 1268 BoNT/F.sub.7-UniParcAccessionNo.UPI00000BAF8C SEQIDNO:15 MPVNINNFNYNDPINNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERN 50 IIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKTVIKLFNRINS 100 NPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESNGTTDSML 150 LNLVILGPGPNILECSTFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEY 200 AFNDNTDLFIADPAISLAHELIHVLHGLYGAKGVTNKKVIEVDQGALMAA 250 EKDIKIEEFITFGGQDLNIITNSTNQKIYVILLSNYTAIASRLSQVNRNN 300 SALNTTYYKNFFQWKYGLDQDSNGNYTVNISKFNAIYKKLFSFTECDLAQ 350 KFQVKNRSNYLFHFKPFRLLDLLDDNIYSISEGFNIGSLRVNNNGQNINL 400 NSRIVGPIPDNGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASES 450 SYNENGINSPKEIDDTTITNNNYKKNLDEVILDYNSDAIPNLSSRLLNTT 500 AQNDSYVPKYDSNGTSEIKEYTVDKLNVFFYLYAQKAPEGESAISLTSSV 550 NTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKS 600 TIDKIADISLIVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELL 650 IPTILVFTIKSFINSDDSKNKIIKAINNALRERELKWKEVYSWIVSNWLT 700 RINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLRAEYNIY 750 SIKEELNKKVSLAMQNIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQ 800 YLLNYILENSSTLGTSSVPELNNLVSNTLNNSIPFELSEYTNDKILIHIL 850 IRFYKRIIDSSILNMKYENNRFIDSSGYGSNISINGDIYIYSTNRNQFGI 900 YSSRLSEVNITQNNTIIYNSRYQNFSVSFWVRIPKYNNLKNLNNEYTIIN 950 CMRNNNSGWKISLNYNNIIWTLQDTTGNNQKLVFNYTQMIDISDYINKWT 1000 FVTITNNRLGHSKLYINGNLTDQKSILNLGNIHVDDNILFKIVGCNDTRY 1050 VGIRYFKIFNMELDKTEIETLYHSEPDSTILKDFWGNYLLYNKKYYLLNL 1100 LKPNMSVTKNSDILNINRQRGIYSKTNIFSNARLYTGVEVIIRKVGSTDT 1150 SNTDNFVRKNDTVYINVVDGNSEYQLYADVSTSAVEKTIKLRRISNSNYN 1200 SNQMIIMDSIGDNCTMNFKTNNGNDIGLLGFHLNNLVASSWYYKNIRNNT 1250 RNNGCFWSFISKEHGWQE 1268 BoNT/F.sub.7-UniParcAccessionNo.UPI0001C0B221 SEQIDNO:16 MPVNINNFNYNDPINNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERN 50 IIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKTVIKLFNRINS 100 NPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESNGTTDSML 150 LNLVILGPGPNILECSTFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEY 200 AFNDNTDLFIADPAISLAHELIHVLHGLYGAKGVTNKKVIEVDQGALMAA 250 EKDIKIEEFITFGGQDLNIVTNSTNQKIYDNLLSNYTAIASRLSQVNINN 300 SALNTTYYKNFFQWKYGLDQDSNGNYTVNISKFNAIYKKLFSFTECDLAQ 350 KFQVKNRSNYLFHFKPFKLLDLLDDNIYSISEGFNIGSLRVNNNGQNINL 400 NSRIVGPIPDNGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASES 450 SYNENGINSPKEIDDTTITNNNYKKNLDEVILDYNSDAIPNLSSRLLNAT 500 AQNDSYVPKYDSNGTSEINEYTVDKLNVFFYLYAQKAPEGESAISLTSSV 550 DTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKS 600 TIDKIADISLVVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELL 650 IPTILVFTIKSFINSDDSKNKIIKAINNALRERELKWKEVYSWIVSNWLT 700 RINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIY 750 NIKEELNKKVSLAMQNIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQ 800 YLLNYILENSSTLGTSSVQELNNLVSNTLNNSIPFELSEYTNDKILISYF 850 NRFYKRIIDSSILNMKYENNRFIDSSGYGSNISINGDIYIYSTNRNQFGI 900 YSSRLSEVNITQNNTIIYNSRYQNFSVSFWVRIPKYNNLKNLNNEYTIIN 950 CMRNNNSGWKISLNYNNIIWTLQDTTGNNQKLVFNYTQMIDISDYINKWT 1000 FVTITNNRLGHSKLYINGNLTDQKSISNLGNIHVDDNILFKIVGCNDTRY 1050 VGIRYFKIFNMELDKTEIETLYHSEPDSTILKDFWGNYLLYNKKYYLLNL 1100 LKPNMSVTKNSDILNINRQRGIYSKTNIFSNARLYTGVEVIIRKFGSTDT 1150 SNTDNFVRKNDTVYISVVDGNSEYQLYADVSTSAVEKTIKLRRISNSNYN 1200 SNQMIIMDSIGDNCTMNFKTNNGNDIGLLGFHLNNLVASSWYYKNIRNNT 1250 RNNGCFWSFISKEHGWQE 1268 BoNT/FconsensussequenceminimalLH.sub.Nbackbone(includingactivationloop) SEQIDNO:17 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTKPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGKVLLXEISXAKPYLGNDHTPINEFXPVNRTTSVNIKLSTNVESSMLLNLLVLGAGPDIFEXS CYPVRKXPNSDXVYDPSNXGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGAXGV TYKETIEVKQXPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIK-YGFLKVPNLLDDDIYTVSEGFN IGNLAVNNRGQNINLNPKIIDSIPDKGLVEKIVKFCKSIIPRKGTKAPPRLCIRVNNRELFFVASESSYNENDINT PKEIDDTTNLNNNYRNNLDEVILDYNSETIPQISNRTLNTLVQDDSYVPRYDSNGTSEIEEYDVVDLNVFFYLHAQ KVPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLI VPYVGLALNIGNEAEKGNFKEAFELLGAGILLEFVPELXIPVILVFTIKSYIDSYENKNKIIKAINNSLIEREAKW KEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEEELNKKVSLA MKNIERFMTESSISYLMKLINEAKVGKLKEYDKHVKSXLLNYILXHRSILGEQ-XQELNDLVTSTLNSSIPFELSS BoNT/F.sub.1-UniParcAccessionNo.UPI00000B66D1minimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:18 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTDPSDFDPPASLENGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGEVLLQEISTAKPYLGNEHTPINEFHPVTRTTSVNIKSSTNVKSSIILNLLVLGAGPDIFENS SYPVRKLMDSGGVYDPSNDGFGSINIVTFSPEYEYTFNDISGGYNSSTESFIADPAISLAHELIHALHGLYGARGV TYKETIKVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSRVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQNIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNRELFFVASESSYNENDINTP KEIDDTTNLNNNYRNNLDEVILDYNSETIPQISNQTLNTLVQDDSYVPRYDSNGTSEIEEHNVVDLNVFFYLHAQK VPEGETNISLTSSIDTALSEESQVYTFFSSEFINTINKPVHAALFISWINQVIRDFTTEATQKSTFDKIADISLVV PYVGLALNIGNEVQKENFKEAFELLGAGILLEFVPELLIPTILVFTIKSFIGSSENKNKIIKAINNSLMERETKWK EIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNNYTSDERNRLESEYNINNIREELNKKVSLAM ENIERFITESSIFYLMKLINEAKVSKLREYDEGVKEYLLDYISEHRSILGNSVQELNDLVTSTLNNSIPFELSS BoNT/F.sub.2-UniParcAccessionNo.UPI00016BB5D7minimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:19 NDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERDTIGTKPDEFQVPDSLKNGSSAYYDPNYLTTDAEKDRYLKT MIKLFNRINSNPTGKVLLEEVSNARPYLGDDDTLINEFFPVNVTTSVNIKFSTDVESSIISNLLVLGAGPDIFKAY CTPLVRFNKSDKLIEPSNHGFGSINILTFSPEYEHIFNDISGGNHNSTESFIADPAISLAHELIHALHGLYGAKAV THKESLVAERGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATRLREVNTAPPGYDINEYKD YFQWKYGLDRNADGSYTVNRNKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFVKVPDLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPKIIDSIPDKGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNNRELFFVASESSYNESDINTP KEIDDTTNLNNNYRNNLDEVILDYNSETIPQISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQK VPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLIV PYVGLALNIVIEAEKGNFEEAFELLGAGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWK EIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESKYNINNIEEELNKKVSLAM KNIERFMTESSISYLMKLINEAEVGKLKEYDKHVKSDLLOYILYHKLILGEQTKELIDLVTSTLMSSIPFELSS BoNT/F.sub.2-UniParcAccessionNo.UPI00000B50F1minimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:20 NDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERDTIGTKPDEFQVPDSLKNGSSAYYDPNYLTTDAEKDRYLKT MIKLFNRINSNPTGKVLLEEVSNARPYLGDDDTLINEFLPVNVTTSVNIKFSTDVESSIISNLLVLGAGPDIFKAY CTPLVRFNKSDKLIEPSNHGFGSINILTFSPEYEHIFNDISGGNHNSTESFIADPAISLAHELIHALHGLYGAKAV THKESLVAERGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATRLREVNTAPPGYDINEYKD YFQWKYGLDRNADGSYTVNRNKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFVKVPNLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPKIIDSIPDKGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNNRELFFVASESSYNESDINTP KEIDDTTNLNNNYRNNLDEVILDYNSETIPQISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQK VPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLIV PYVGLALNIVIEAEKGNFEEAFELLGAGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWK EIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESKYNINNIEEELNKKVSLAM KNIERFMTESSISYLMKLINEAEVGKLKEYDKHVKSDLLDYILYHKLILGEQTKELIDLVTSTLNSSIPFELSS BoNT/F.sub.3-UniParcAccessionNo.UPI0001C0B12FminimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:21 NDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERDTIGTKPDDFQVPDSLKNGSSAYYDPNYLTTDAEKDRYLKT MIKLFNRINSNPTGKVLLEEVSNARPYLGDDDTLINEFFPVNVTTSVNIKFSTDVESSIISNLLVLGAGPDIFKAY CTPLVRFNKSDKLIEPSNHGFGSINILTFSPEYEHIFNDISGGDHNSTESFIADPAISLAHELIHALHGLYGAKAV THKETIEVKRGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATRLREVNTAPPEYDINEYKD YFQWKYGLDRNADGSYTVNRNKFNGIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFVKVPDLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPKIIDSIPDKGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNNRELFFVASESSYNESDINTP KEIDDTTNLNNNYRNNLDEVILDYNSETIPQISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQK VPEGETNISLTSSIDTALLEKSKVYTFFSSEFIDTINESVNAALFIDWINKVIRDFTTEATQKSTVDKIADISLIV PYVGLALNIVIDAEKGNFQEAFELLGAGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNALIEREAKWK EIYSWIVSNWLTKINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEEELNKKVSLAM KNIERFMTESSISYLMKLINEAEVGKLKKYDRHVKSDLLDYILYHKLILGDQTKELIDLVTSTLNSSIPFELSS BoNT/F.sub.4-UniParcAccessionNo.UPI0001A92658minimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:22 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIMPERNTIGINPSDFDPPASLKNGSSAYYDPNYLITDAEKDRYLKT TIKLFKRINSNPAGEVLLQEISTAKPYLGNDHIPINEFHPVIRTTSVNIKSSTNVESSIILNLLVLGAGPNIFENS SYPVRKLMNSGEVYDPSNOGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYKETIKVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNDLLANYEKIATRLSEVNSAPPEYDINEYKN YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPKIIDSIPDKGLVEKIVKLCKSIIPRKGTKAPPRLCIRVNNRELFFVASESSYNENDINTP KEIDDTTNLNNNYRNNLDEVILDYNSETIPQISSQTLNTLVQDDSYVPRYDSNGTSEIEEHNVVDLNAFFYLHAQK VPEGETNISLTSSIDTALSEESKVYTFFSSEFINNINKPVHAALFIGWISQVIRDFTTESTQKSTVDKIADISLIV PYVGLALNIGNDARKGNFKEAFELLGAAILLEVVPELLIPVILVFTIKSFIDSSKNEDKIIKAINNSLIEREAKWK EVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNSYTSDEKNRLESEYNINNIEEELNKKVSLAM KNIERFIAESSISYLMKLINEAKVSELREYDEGVKEYLLDYILKNGSILGDHVQELNDLVTSTLNSSIPFELSS BoNT/F.sub.4-UniParcAccessionNo.UPI0001C0B12DminimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:23 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIMPERNTIGINPSDFDPPASLKNGSSAYYDPNYLITDAEKDRYLKT TIKLFKRINSNPAGEVLLQEISTAKPYLGNDHIPINEFHPVIRTTSVNIKSSTNVESSIILNLLVLGAGPNIFENS SYPVRKLMNSGEVYDPSNDGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYKETIKVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNDLLANYEKIATRLSEVNSAPPEYDINEYKN YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPKIIDSIPDKGLVEKIVKLCKSIIPRKGTKAPPRLCIRVNNRELFFVASESSYNENDINTP KEIDDTTNLNNNYRNNLDEVILDYNSETIPQISSQTLNTLVQDDSYVPRYDSNGTSEIEEHNVVDLNAFFYLHAQK VPEGETNISLTSSIDTALSEESKVYTFFSSEFINNINKPVHAALFIGWISQVIRDFTTESTQKSTVDKIADISLIV PYVGLALNIGNDARKGNFKEAFELLGAAILLEVVPELLIPVILVFTIKSFIDSSKNEDKIIKAINNSLIEREAKWK EVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNSYTSDEKNRLESEYNINNIEEELNKKVSLAM KNIERFIAESSISYLMKLINEAKVSELREYDEGVKEYLLDYILKNGSILGOHVQELNDLVTSTLMSSIPFELSS BoNT/F.sub.5-UniParcAccessionNo.UPI0001C0B12EminimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:24 NDNTILYIRPPYYERSNTYFKAFNIMENVWIIPERYRLGIEASKFDPPDSLKAGSDGYFDPNYLSTNTEKNRYLQI MIKLFKRINSNEAGKILLNQIKDAIPYLGNSYTAEDQFTTNNRTISFNVRLANGTIEQEMANLIIWGPGPDLITNR TGGTTYTPAQSLEAIPYKEGFGSIMTIEFSPEYATAFNDISLTSHAPSLFIKDPALILMHELIHVLHGLYGTYTTG FKIKPNITEPYMEVTKPITSGEFLTFGGNDVNKIPQLIQSQLRSKVLDDYEKIASRLNKVNRATAEINIDKFKYSY QLKYQFVKDSNGVYSVDLDKFNKLYDKIYSFTEFNLAHEFKIKTRNSYLAKNFGPFYLPNLLDNSIYNEADGFNIG DLSVNYKGQVIGSDIDSIKKLEGQGVVSRVVRLCLNSSFKKNTKKPLCITVNNGDLFFIASEDSYGEDTINTPKEI DDTTTLVPSFKNILDKVILDFNKQVIPQIPNRRIRTDIQEDNYIPEYDSNGTSEIEEYNVVDLNAFFYLHAQKVPE GETNISLTSSIDTALSEESKVYTFFSSEFIDTINEPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLIVPYV GLALNIVNETEKGNFKEAFELLGAGILLEFVPELAIPVILVFTIKSYIDSYENKNKIIKAINNSLIEREAKWKEIY SWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEEELNKKVSLAMKNI ERFITESSISYLMKLINEAEVGKLKEYDKRVKRHLLEYIFDYRLILGEQGGELIDLVISTLNISIPFELSS BoNT/F.sub.6-UniParcAccessionNo.UPI0001C0B1D1minimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:25 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGKVLLQEISTAKPYLGNDHIPIDEFSPVIRTTSVNIKLSTNVESSMLLNLLVLGAGPDIFESC CYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYEETIEVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTESDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNSELFFVASESSYNENDINTP KEIDDTTNLNNNYRNNLDEVILDYNSQTIPQISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQK VPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLIV PYVGLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWK EIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEEELNKKVSLAM KNIERFMTESSISYLMKLINEAKVGKLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSS BoNT/F.sub.6-UniParcAccessionNo.UPI0000126B8AminimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:26 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGKVLLQEISTAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSMLLNLLVLGAGPDIFESC CYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYEETIEVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTESDLANKFKVKCRNTYFIKYEFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNSELFFVASESSYNENDINTP KEIDDTTNLNNNYRNNLDEVILDYNSQTIPQISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQK VPEGETNISLTSSIDTALLEESKDIFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLIVP YVGLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWKE IYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEEELNKKVSLAMK NIERFMTESSISYLMKLINEAKVGKLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSS BoNT/F.sub.6-UniParcAccessionNo.UPI0001C0B1D0minimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:27 NDDTILYMQIPYKEKSKKYYKAFEIMRNVWIIPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGKVLLQEISYAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSMLLNLLVLGAGPDIFESC CYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYEETIEVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTESDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNSELFFVASESSYNENDINTP KEIDDTTNLNNNYRNNLDEVILDYNSQTIPQISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQK VPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLIV PYVGLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWK EIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEEELNKKVSLAM KNIERFMTESSISYLMKLINEAKVGKLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSS BoNT/F.sub.6-UniParcAccessionNo.UPI0004E7C8CEminimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:28 NDDTILYMQIPYKEKSKKYYKAFEIMRNVWIIPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGKVLLQEISTAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSMLLNLLVLGAGPDIFESC CYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYEETIEVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTESDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNSELFFVASESSYNENDINTP KEIDDTTNLNNNYRNNLDEVILDYNSQTIPQISNRTLNTLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQK VPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLIV PYVGLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNSLIEREAKWK EIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEEELNKKVSLAM KNIERFMTESSISYLMKLINEAKVGKLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSS BoNT/F.sub.7-UniParcAccessionNo.UPI0001DE3DACminimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:29 NNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERNIIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKT VIKLFNRINSNPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESKGTTDSMLLNLVILGPGPNILECS TFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEYAFNDNTDLFIADPAISLAHELIHVLHGLYGAKGVTNKKVIEV DQGALMAAEKDIKIEEFITFGGQDLNIITNSTNQKIYDNLLSNYTAIASRLSQVNINNSALNTTYYKNFFQWKYGL DQDSNGNYTVNISKFNAIYKKLFSFTECDLAQKFQVKNRSNYLFHFKPFRLLDLLDDNIYSISEGFNIGSLRVNNN GQNINLNSRIVGPIPDNGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASESSYNENGINSPKEIDDTTITN NNYKKNLDEVILDYNSDAIPNLSSRLLNTTAQNDSYVPKYDSNGTSEIKEYTVDKLNVFFYLYAQKAPEGESAISL TSSVNTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTIDKIADISLVVPYVGLALNIG NEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINNALRERELKWKEVYSWIVSNW LTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIYSIKEELNKKVSLAMQNIDRFLTES SISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVQELNNLVSNTLNNSIPFELSE BoNT/F.sub.7-UniParcAccessionNo.UPI0001C0B220minimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:30 NNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERNIIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKT VIKLFNRINSNPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESNGTTDSMLLNLVILGPGPNILECS TFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEYAFNDNTDLFIADPAISLAHELIHVLHGLYGAKGVTNKKVIEV DQGALMAAEKDIKIEEFITFGGQDLNIITNSTNQKIYDNLLSNYTAIASRLSQVNINNSALNTTYYKNFFQWKYGL DQDSNGNYTVNISKFNAIYKKLFSFTECDLAQKFQVKNRSNYLFHFKPFRLLDLLDDNIYSISEGFNIGSLRVNNN GQNINLNSRIVGPIPDNGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASESSYNENGINSPKEIDDTTITN NNYKKNLDEVILDYNSDAIPNLSSRLLNTTAQNDSYVPKYDSNGTSEIKEYTVDKLNVFFYLYAQKAPEGESAISL TSSVNTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTIDKIADISLVVPYVGLALNIG NEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINNALRERELKWKEVYSWIVSNW LTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIYSIKEELNKKVSLAMQNIDRFLTES SISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVQELNNLVSNTLNNSIPFELSE BoNT/F.sub.7-UniParcAccessionNo.UPI00000BAF8CminimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:31 NNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERNIIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKT VIKLFNRINSNPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESNGTIDSMLLNLVILGPGPNILECS TFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEYAFNDNIDLFIADPAISLAHELIHVLHGLYGAKGVTNKKVIEV DQGALMAAEKDIKIEEFITFGGQDLNIITNSTMQKIYVILLSNYTAIASRLSQVNRMNSALNTTYYKNFFQWKYGL DQDSNGNYTVNISKFNAIYKKLFSFTECDLAQKFQVKNRSNYLFHFKPFRLLDLLDDNIYSISEGFNIGSLRVNNN GQNINLNSRIVGPIPDNGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASESSYNENGINSPKEIDDTTITN NNYKKNLDEVILDYNSDAIPNLSSRLLNTTAQNDSYVPKYDSNGTSEIKEYTVDKLNVFFYLYAQKAPEGESAISL TSSVNTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTIDKIADISLIVPYVGLALNIG NEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINNALRERELKWKEVYSWIVSNW LTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLRAEYNIYSIKEELNKKVSLAMQNIDRFLTES SISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVPELNNLVSNTLNNSIPFELSE BoNT/F.sub.7-UniParcAccessionNo.UPI0001C0B221minimalLH.sub.Nbackbone (includingactivationloop) SEQIDNO:32 NNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERNIIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKT VIKLETRINSNPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESNGTIDSMLLNLVILGPGPNILECS TFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEYAFNDNIDLFIADPAISLAHELIHVLHGLYGAKGVTNKKVIEV DQGALMAAEKDIKIEEFITFGGQDLNIVTNSTNQKIYDNLLSNYTAIASRLSQVNINNSALNITYYKNEFQWKYGL DQDSNGNYTVNISKFNAIYKKLFSFTECDLAQKFQVKNRSNYLFHFKPFKLLDLLDDNIYSISEGFNIGSLRVNNN GQNINLNSRIVGPIPDNGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASESSYNENGINSPKEIDDTTITN NNYKKNLDEVILDYNSDAIPNLSSRLLNATAQNDSYVPKYDSNGTSEINEYTVDKLNVFFYLYAQKAPEGESAISL TSSVDTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTIDKIADISLVVPYVGLALNIG NEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINNALRERELKWKEVYSWIVSNW LTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIYNIKEELNKKVSLAMQNIDRFLTES SISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVQELNNLVSNTLNNSIPFELSE BoNT/FconsensussequenceminimalLchainfragment SEQIDNO:33 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTKPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGKVLLXEISXAKPYLGNDHTPINEFXPVNRTTSVNIKLSTNVESSMLLNLLVLGAGPDIFEXS CYPVRKXPNSDXVYDPSNXGFGSINIVTFSPEYEYTENDISGGHNSSTESFIADPAISLAHELIHALHGLYGAXGV TYKETIEVKQXPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWKYGIAKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIK-YGFLKVPNLLDDDIYTVSEGFN IGNLAVNNRGQNINLNPK BoNT/F.sub.1-UniParcAccessionNo.UPI00000B66D1minimalLchainfragment SEQIDNO:34 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTDPSDFDPPASLENGSSAYYDPNYLITDAEKDRYLKT TIKLFKRINSNPAGEVLLQEISYAKPYLGNEHTPINEFHPVIRTTSVNIKSSTNVKSSIILNLLVLGAGPDIFENS SYPVRKLMDSGGVYDPSNDGEGSINIVTESPEYEYTENDISGGYNSSTESFIADPAISLAHELIHALHGLYGARGV TYKETIKVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSRVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQNIKLNPK BoNT/F.sub.2-UniParcAccessionNo.UPI00016BB5D7minimalLchainfragment SEQIDNO:35 NDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERDTIGTKPDEFQVPDSLKNGSSAYYDPNYLTIDAEKDRYLKT MIKLENRINSNPTGKVLLEEVSNARPYLGDDDTLINEFFPVNVTTSVNIKFSTDVESSIISNLLVLGAGPDIFKAY CIPLVRFNKSDKLIEPSNHGEGSINILTESPEYEHIENDISGGNHNSTESFIADPAISLAHELIHALHGLYGAKAV THKESLVAERGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATRLREVNTAPPGYDINEYKD YFQWKYGLDRNADGSYTVNRNKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFVKVPDLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPK BoNT/F.sub.2-UniParcAccessionNo.UPI00000B50F1minimalLchainfragment SEQIDNO:36 NDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERDTIGTKPDEFQVPDSLKNGSSAYYDPNYLTTDAEKDRYLKT MIKLFNRINSNPTGKVLLEEVSNARPYLGDDDTLINEFLPVNVTTSVNIKFSTDVESSIISNLLVLGAGPDIFKAY CTPLVRFNKSDKLIEPSNHGFGSINILTFSPEYEHIFNDISGGNHNSTESFIADPAISLAHELIHALHGLYGAKAV THKESLVAERGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATRLREVNTAPPGYDINEYKD YFQWKYGLDRNADGSYTVNRNKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFVKVPNLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPK BoNT/F.sub.3-UniParcAccessionNo.UPI0001C0B12FminimalLchainfragment SEQIDNO:37 MDETILYMQKPYEERSRKYYKAFEIMPNVWIMPERDTIGTKPODFQVPDSLKNGSSAYYDPNYLTTDAEKDRYLKT MIKLFNRINSNPTGKVLLEEVSNARPYLGDDDTLINEFFPVNVTTSVNIKFSTDVESSIISNLLVLGAGPDIFKAY CTPLVRFNKSDKLIEPSNHGFGSINILTFSPEYEHIFNDISGGDHNSTESFIADPAISLAHELIHALHGLYGAKAV THKETIEVKRGPLMIAEKPIRLEEFLTFGGEDLNIIPSAMKEKIYNDLLANYEKIATRLREVNTAPPEYDINEYKD YFQWKYGLDRNADGSYTVNRNKFNGIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFVKVPDLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPK BoNT/F.sub.4-UniParcAccessionNo.UPI0001A92658minimalLchainfragment SEQIDNO:38 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIMPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGEVLLQEISYAKPYLGNDHTPINEFHPVTRTTSVNIKSSTNVESSIILNLLVLGAGPNIFENS SYPVRKLMNSGEVYDPSNDGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYKETIKVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNDLLANYEKIATRLSEVNSAPPEYDINEYKN YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPK BoNT/F.sub.4-UniParcAccessionNo.UPI0001C0B12DminimalLchainfragment SEQIDNO:39 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIMPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGEVLLQEISYAKPYLGNDHTPINEFHPVTRTTSVNIKSSTNVESSIILNLLVLGAGPNIFENS SYPVRKLMNSGEVYDPSNDGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYKETIKVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNDLLANYEKIATRLSEVNSAPPEYDINEYKN YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQNINLNPK BoNT/F.sub.5-UniParcAccessionNo.UPI0001C0B12EminimalLchainfragment SEQIDNO:40 NDNTILYIRPPYYERSNTYFKAFNIMENVWIIPERYRLGIEASKFDPPDSLKAGSDGYFDPNYLSTNTEKNRYLQI MIKLFKRINSNEAGKILLNQIKDAIPYLGNSYTAEDQFTTNNRTISFNVRLANGTIEQEMANLIIWGPGPDLTTNR TGGTTYTPAQSLEAIPYKEGFGSIMTIEFSPEYATAFNDISLTSHAPSLFIKDPALILMHELIHVLHGLYGTYTTG FKIKPNITEPYMEVTKPITSGEFLTFGGNDVNKIPQLIQSQLRSKVLDDYEKIASRLNKVNRATAEINIDKFKYSY QLKYQFVKDSNGVYSVDLDKFNKLYDKIYSFTEFNLAHEFKIKTRNSYLAKNFGPFYLPNLLDNSIYNEADGFNIG DLSVNYKGQVIGSDID BoNT/F.sub.6-UniParcAccessionNo.UPI0001C0B1D1minimalLchainfragment SEQIDNO:41 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGKVLLQEISYAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSMLLNLLVLGAGPDIFESC CYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYEETIEVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTESDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQSIKLNPK BoNT/F.sub.6-UniParcAccessionNo.UPI0000126B8AminimalLchainfragment SEQIDNO:42 NDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKT TIKLFKRINSNPAGKVLLQEISYAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSMLLNLLVLGAGPDIFESC CYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYEYTFNDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYEETIEVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTESDLANKFKVKCRNTYFIKYEFLKVPNLLDDDIYTVSEGFNI GNLAVNNRGQSIKLNPK BoNT/F.sub.6-UniParcAccessionNo.UPI0001C0B1D0minimalLchainfragment SEQIDNO:43 NEDTILYMQIPYKEKSKEYYKAFEIMPNVWIIPERNTIGINPSDFDPPASLENGSSAYYDPNYLTTDAEKDRYLKT TIELFKRINSNPAGEVLLQEISYAKPYLGNEHTPIDEFSPVTPTTSVNIKLSTNVESSMLLNLLVLGAGPDIFESC CYPVRKLIDPDVVYDPSNYGEGSINIVTESPEYEYTENDISGGENSSTESFIADPAISLAHELIHALEGLYGARGV TYEETIEVKQAPLMIAEKPIRLEEFLTEGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWEYGLDKNADGSYTVNENKFNEIYKKLYSFTESDLANKFEVECRNTYFIKYGFLKVPNLLDEDIYTVSEGFNI GNLAVNNRGQSIKLNPK BoNT/F.sub.6-UniParcAccessionNo.UPI0004E7C8CEminimalLchainfragment SEQIDNO:44 NEDTILYMQIPYKEKSKEYYKAFEIMPNVWIIPERNTIGINPSDFDPPASLENGSSAYYDPNYLTTDAEKDRYLKT TIELFKRINSNPAGEVLLQEISYAKPYLGNEHTPIDEFSPVTRTTSVNIKLETNVESSMLLNLLVLGAGPDIFESC CYPVRKLIDPDVVYDPSNYGEGSINIVTESPEYEYTENDISGGHNSSTESFIADPAISLAHELIHALHGLYGARGV TYEETIEVKQAPLMIAEKPIRLEEFLTEGGQDLNIITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKD YFQWEYGLDKNADGSYTVNENKFNEIYKKLYSFTESDLANKFEVECRNTYFIKYGFLKVPNLLEDDITIVSEGFNI GNLAVNNRGQSIKLNPK BoNT/F.sub.7-UniParcAccessionNo.UPI0001DE3DACminimalLchainfragment SEQIDNO:45 NNTTILYMKMPYYEDSNKYYKAFEIMENVWIIPERNIIGKEPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLET VIKLENRINSNPAGQVLLEEIENGKPYLGNEHTAVNEFCANNRSTSVEIKESEGTTESMLLNLVILGPGPNILECS TFPVRIFPNNIAYDPSEKGEGSIQLMSFSTEYEYAFNENTELFIADPAISLAHELIHVLHGLYGAKGVINKKVIEV DQGALMAAEKDIKIEEFITEGGQDLNIITNSTNQKIYDNLLSNYTAIASRLSQVNINNSALNITYYKNEFQWEYGL DQDSNGNYTVNISKFNAIYKKLFSFTECELAQKFQVENRSNYLFHFKPFRLLDLLEDNIYSISEGFNIGSLRVNNN GQNINLNSR BoNT/F.sub.7-UniParcAccessionNo.UPI0001C0B220minimalLchainfragment SEQIDNO:46 NNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERNIIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLKT VIKLENRINSNPAGQVLLEEIENGEPYLGNEHTAVNEFCANNRSTSVEIKESNGTTESMLLNLVILGPGPNILECS TFPVRIFPNNIAYDPSEKGEGSIQLMSFSTEYEYAFNENTELFIADPAISLAHELIHVLEGLYGAKGVINKKVIEV DQGALMAAEKDIKIEEFITEGGQDLNIITNSTNQKIYDNLLSNYTAIASRLSQVNINNSALNITYYKNEFQWEYGL DQDSNGNYTVNISKFNAIYKKLFSFTECELAQKFQVENRSNYLFHFKPFRLLDLLEDNIYSISEGFNIGSLRVNNN GQNINLNSR BoNT/F.sub.7-UniParcAccessionNo.UPI00000BAF8CminimalLchainfragment SEQIDNO:47 NNTTILYMEMPYYEDSNKYYKAFEIMENVWIIPERNIIGKEPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLET VIKLENRINSNPAGQVLLEEIENGEPYLGNEHTAVNEFCANNRSTSVEIKESNGTTESMLLNLVILGPGPNILECS TFPVRIFPNNIAYDPSEKGEGSIQLMSFSTEYEYAFNENTELFIADPAISLAHELIHVLHGLYGAKGVINKKVIEV DQGALMAAEKDIKIEEFITEGGQDLNIITNSTNQKIYVILLSNYTAIASRLSQVNRNNSALNITYYKNEFQWEYGL DQESNGNYTVNISKFNAIYKKLFSFTECELAQKFQVKNRSNYLFHFKPFRLLDLLEDNIYSISEGFNIGSLRVNNN GQNINLNSR BoNT/F.sub.7-UniParcAccessionNo.UPI0001C0B221minimalLchainfragment SEQIDNO:48 NNTTILYMKMPYYEDSNKYYKAFEIMENVWIIPERNIIGKKPSDFYPPISLDSGSSAYYDPNYLTTDAEKDRFLET VIKLENRINSNPAGQVLLEEIENGKPYLGNEHTAVNEFCANNRSTSVEIKESNGTTESMLLNLVILGPGPNILECS TFPVRIFPNNIAYDPSEKGEGSIQLMSFSTEYEYAFNENTELFIADPAISLAHELIHVLHGLYGAKGVINKKVIEV DQGALMAAEKDIKIEEFITFGGQDLNIVTNSTMQKIYDNLLSNYTAIASRLSQVNINNSALNTTYYKNFFQWKYGL DQESNGNYTVNISKFNAIYKKLFSFTECELAQKFQVKNRSNYLFHFKPFKLLDLLEDNIYSISEGFNIGSLRVNNN GQNINLNSR BoNT/FconsensussequenceminimalH.sub.Nfragment SEQIDNO:49 NVFFYLHAQKVPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIREFTTEATQKSTV DKIADISLIVPYVGLALNIGNEAEKGNFKEAFELLGAGILLEFVPELXIPVILVFTIKSYIDSYENKNKIIKAINN SLIEREAKWKEIYSWIVSNWLTRINTQFNERKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEE ELNKKVSLAMKNIERFMTESSISYLMKLINEAKVGKLKEYEKHVESXLLNYILXHRSILGEQ-XQELNDLVISTLN SSIPFELSS BoNT/F.sub.1-UniParcAccessionNo.UPI00000B66D1minimalH.sub.Nfragment SEQIDNO:50 NVFFYLHAQKVPEGETNISLTSSIDTALSEESQVYTFFSSEFINTINKPVHAALFISWINQVIRDFTTEATQKSTF DKIADISLVVPYVGLALNIGNEVQKENFKEAFELLGAGILLEFVPELLIPTILVFTIKSFIGSSENKNKIIKAINN SLMERETKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNNYTSDERNRLESEYNINNIRE ELNKKVSLAMENIERFITESSIFYLMKLINEAKVSKLREYDEGVKEYLLDYISEHRSILGNSVQELNDLVTSTLNN SIPFELSS BoNT/F.sub.2-UniParcAccessionNo.UPI00016BB5D7minimalH.sub.Nfragment SEQIDNO:51 NVFFYLHAQKVPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTV DKIADISLIVPYVGLALNIVIEAEKGNFEEAFELLGAGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINN SLIEREAKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESKYNINNIEE ELNKKVSLAMKNIERFMTESSISYLMKLINEAEVGKLKEYDKHVKSDLLDYILYHKLILGEQTKELIDLVTSTLNS SIPFELSS BoNT/F.sub.2-UniParcAccessionNo.UPI00000B50F1minimalH.sub.Nfragment SEQIDNO:52 NVFFYLHAQKVPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTV DKIADISLIVPYVGLALNIVIEAEKGNFEEAFELLGAGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINN SLIEREAKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESKYNINNIEE ELNKKVSLAMKNIERFMTESSISYLMKLINEAEVGKLKEYDKHVKSDLLDYILYHKLILGEQTKELIDLVTSTLNS SIPFELSS BoNT/F.sub.3-UniParcAccessionNo.UPI0001C0B12FminimalH.sub.Nfragment SEQIDNO:53 NVFFYLHAQKVPEGETNISLTSSIDTALLEKSKVYTFFSSEFIDTINESVNAALFIDWINKVIRDFTTEATQKSTV DKIADISLIVPYVGLALNIVIDAEKGNFQEAFELLGAGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINN ALIEREAKWKEIYSWIVSNWLTKINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEE ELNKKVSLAMKNIERFMTESSISYLMKLINEAEVGKLKKYDRHVKSDLLDYILYHKLILGDQTKELIDLVTSTLNS SIPFELSS BoNT/F.sub.4-UniParcAccessionNo.UPI0001A92658minimalH.sub.Nfragment SEQIDNO:54 NAFFYLHAQKVPEGETNISLTSSIDTALSEESKVYTFFSSEFINNINKPVHAALFIGWISQVIRDFTTESTQKSTV DKIADISLIVPYVGLALNIGNDARKGNFKEAFELLGAAILLEVVPELLIPVILVFTIKSFIDSSKNEDKIIKAINN SLIEREAKWKEVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNSYTSDEKNRLESEYNINNIEE ELNKKVSLAMKNIERFIAESSISYLMKLINEAKVSELREYDEGVKEYLLDYILKNGSILGDHVQELNDLVTSTLNS SIPFELSS BoNT/F.sub.4-UniParcAccessionNo.UPI0001C0B12DminimalH.sub.Nfragment SEQIDNO:55 NAFFYLHAQKVPEGETNISLTSSIDTALSEESKVYTFFSSEFINNINKPVHAALFIGWISQVIRDFTTESTQKSTV DKIADISLIVPYVGLALNIGNDARKGNFKEAFELLGAAILLEVVPELLIPVILVFTIKSFIDSSKNEDKIIKAINN SLIEREAKWKEVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNSYTSDEKNRLESEYNINNIEE ELNKKVSLAMKNIERFIAESSISYLMKLINEAKVSELREYDEGVKEYLLDYILKNGSILGDHVQELNDLVTSTLNS SIPFELSS BoNT/F.sub.5-UniParcAccessionNo.UPI0001C0B12EminimalH.sub.Nfragment SEQIDNO:56 NAFFYLHAQKVPEGETNISLTSSIDTALSEESKVYTFFSSEFIDTINEPVNAALFIDWISKVIRDFTTEATQKSTV DKIADISLIVPYVGLALNIVNETEKGNFKEAFELLGAGILLEFVPELAIPVILVFTIKSYIDSYENKNKIIKAINN SLIEREAKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEE ELNKKVSLAMKNIERFITESSISYLMKLINEAEVGKLKEYDKRVKRHLLEYIFDYRLILGEQGGELIDLVTSTLNT SIPFELSS BoNT/F.sub.6-UniParcAccessionNo.UPI0001C0B1D1minimalH.sub.Nfragment SEQIDNO:57 NVFFYLHAQKVPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTV DKIADISLIVPYVGLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINN SLIEREAKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEE ELNKKVSLAMKNIERFMTESSISYLMKLINEAKVGKLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNS SIPFELSS BoNT/F.sub.6-UniParcAccessionNo.UPI0000126B8AminimalH.sub.Nfragment SEQIDNO:58 NVFFYLHAQKVPEGETNISLTSSIDTALLEESKDIFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVD KIADISLIVPYVGLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINNS LIEREAKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEEE LNKKVSLAMKNIERFMTESSISYLMKLINEAKVGKLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSS IPFELSS BoNT/F.sub.6-UniParcAccessionNo.UPI0001C0B1D0minimalH.sub.Nfragment SEQIDNO:59 NVFFYLHAQKVPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTV DKIADISLIVPYVGLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINN SLIEREAKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEE ELNKKVSLAMKNIERFMTESSISYLMKLINEAKVGKLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNS SIPFELSS BoNT/F.sub.6-UniParcAccessionNo.UPI0004E7C8CEminimalH.sub.Nfragment SEQIDNO:60 NVFFYLHAQKVPEGETNISLTSSIDTALLEESKVYTFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTV DKIADISLIVPYVGLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAIKAINN SLIEREAKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYKYNNYTSDEKNRLESEYNINNIEE ELNKKVSLAMKNIERFMTESSISYLMKLINEAKVGKLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNS SIPFELSS BoNT/F.sub.7-UniParcAccessionNo.UPI0001DE3DACminimalH.sub.Nfragment SEQIDNO:61 NVFFYLYAQKAPEGESAISLTSSVNTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTI DKIADISLVVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINN ALRERELKWKEVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIYSIKE ELNKKVSLAMUIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVIDELNNLVSNTLN NSIPFELSE BoNT/F.sub.7-UniParcAccessionNo.UPI0001C0B220minimalH.sub.Nfragment SEQIDNO:62 NVFFYLYAQKAPEGESAISLTSSVNTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTI DKIADISLVVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINN ALRERELKWKEVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIYSIKE ELNKKVSLAMQNIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVQELNNLVSNTLN NSIPFELSE BoNT/F.sub.7-UniParcAccessionNo.UPI00000BAF8CminimalH.sub.Nfragment SEQIDNO:63 NVFFYLYAQKAPEGESAISLTSSVNTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTI DKIADISLIVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINN ALRERELKWKEVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLRAEYNIYSIKE ELNKKVSLAMQNIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVPELNNLVSNTLN NSIPFELSE BoNT/F.sub.7-UniParcAccessionNo.UPI0001C0B221minimalH.sub.Nfragment SEQIDNO:64 NVFFYLYAQKAPEGESAISLTSSVDTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTI DKIADISLVVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINN ALRERELKWKEVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIYNIKE ELNKKVSLAMQNIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVQELNNLVSNTLN NSIPFELSE BoNT/Fconsensussequenceminimalactivationloop(alsoactivation loopsequenceforUPI0001A92658(BoNT/F.sub.4)andUPI0001C0B12D(BoNT/F.sub.4)) SEQIDNO:65 KSIIPRKGTKAPPRL BoNT/F.sub.1-UniParcAccessionNo.UPI00000B66D1minimal activationloop(alsoactivationloopsequenceforUPI0001C0B1D1(BoNT/F.sub.6), UPI0000126B8A(BoNT/F.sub.6),UPI0001C0B1D0(BoNT/F.sub.6)andUPI0004E7C8CE(BoNT/F.sub.6)) SEQIDNO:66 KSVIPRKGTKAPPRL BoNT/F.sub.2-UniParcAccessionNo.UPI00016BB5D7minimal activationloop(alsoactivationloopsequenceforUPI00000B50F1(BoNT/F.sub.2) andUPI0001C0B12F(BoNT/F.sub.3)) SEQIDNO:67 KSIIPRKGTKQSPSL BoNT/F.sub.5-UniParcAccessionNo.UPI0001C0B12Eminimalactivationloop SEQIDNO:68 LNSSFKKNTKKPL BoNT/F.sub.7-UniParcAccessionNo.UPI0001DE3DACminimal activationloop(alsoactivationloopsequenceforUPI0001C0B220(BoNT/F.sub.7), UPI00000BAF8C(BoNT/F.sub.7)andUPI0001C0B221(BoNT/F.sub.7)) SEQIDNO:69 KSIVSKKGTKNSL F1/4/6activationloopsequence(aftercleavagesite)+ startofF7heavy chainsequence SEQIDNO:70 APPRLCIK F1/4/6activationloopsequence(aftercleavagesite)+ startofF5heavy chainsequence SEQIDNO:71 APPRLCIT F2/3activationloopsequence(aftercleavagesite)+ startofF7heavy chainsequence SEQIDNO:72 QSPSLCIK F2/3activationloopsequence(aftercleavagesite)+ startofF5heavy chainsequence SEQIDNO:73 QSPSLCIT F1/4/6activationloopsequence(aftercleavagesite)+ startofF7heavy chainsequence SEQIDNO:74 APPRLCIKV full-lengthrecombinantBoNT/F7-1(SXN102875_rBoNT-F7-1) SEQIDNO:75 MPVNINNFNYNDPINNTTILYMKMPYYEDSNKYYKAFEIMDNVWIIPERNIIGKKPSDFYPPISLDSGSSAYYDPN YLTTDAEKDRFLKTVIKLFNRINSNPAGQVLLEEIKNGKPYLGNDHTAVNEFCANNRSTSVEIKESKGTTDSMLLN LVILGPGPMILECSTFPVRIFPNNIAYDPSEKGFGSIQLMSFSTEYEYAFNDNTDLFIADPAISLAHELIHVLHGL YGAKGVTNKKVIEVDQGALMAAEKDIKIEEFITFGGQDLNIITNSTNQKIYDNLLSNYTAIASRLSQVNINNSALN TTYYKNFFQWKYGLDQDSNGNYTVNISKFNAIYKKLFSFTECDLAQKFQVKNRSNYLFHFKPFRLLDLLDDNIYSI SEGFNIGSLRVNNNGQNINLNSRIVGPIPDNGLVERFVGLCKSVIPRKGTKAPPRLCIKVNNRDLFFVASESSYNE NGINSPKEIDDTTITNNNYKKNLDEVILDYNSDAIPNLSSRLLNTTAQNDSYVPKYDSNGTSEIKEYTVDKLNVFF YLYAQKAPEGESAISLTSSVNTALLDASKVYTFFSSDFINTVNKPVQAALFISWIQQVINDFTTEATQKSTIDKIA DISLVVPYVGLALNIGNEVQKGNFKEAIELLGAGILLEFVPELLIPTILVFTIKSFINSDDSKNKIIKAINNALRE RELKWKEVYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDGIKKIIEYKYNNYTLDEKNRLKAEYNIYSIKEELNK KVSLAMQNIDRFLTESSISYLMKLINEAKINKLSEYDKRVNQYLLNYILENSSTLGTSSVQELNNLVSNTLNNSIP FELSEYTNDKILISYFNRFYKRIIDSSILNMKYENNRFIDSSGYGSNISINGDIYIYSTNRNQFGIYSSRLSEVNI TQNNTIIYNSRYQNFSVSFWVRIPKYNNLKNLNNEYTIINCMRNNNSGWKISLNYNNIIWTLQDTTGNNQKLVFNY TQMIDISDYINKWTFVTITNNRLGHSKLYINGNLTDQKSILNLGNIHVDDNILFKIVGCNDTRYVGIRYFKIFNME LDKTEIETLYHSEPDSTILKDFWGNYLLYNKKYYLLNLLKPNMSVTKNSDILNINRQRGIYSKTNIFSNARLYTGV EVIIRKVGSTDTSNTDNFVRKNDTVYINVVDGNSEYQLYADVSTSAVEKTIKLRRISNSNYNSNQMIIMDSIGDNC TMNFKTNNGNDIGLLGFHLNNLVASSWYYKNIRNNTRNNGCFWSFISKEHGWQE UPI0000001386(BoNT/A.sub.1)sequencespanningactivationloop SEQIDNO:76 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0000ED909E(BoNT/A.sub.1)sequencespanningactivationloop SEQIDNO:77 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0000EF85BD(BoNT/A.sub.1)sequencespanningactivationloop SEQIDNO:78 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI00016529B7(BoNT/A.sub.1)sequencespanningactivationloop SEQIDNO:79 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI00027EE164(BoNT/A.sub.1)sequencespanningactivationloop SEQIDNO:80 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI000003409D(BoNT/A.sub.1)sequencespanningactivationloop SEQIDNO:81 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0002AA7E5E(BoNT/A.sub.1)sequencespanningactivationloop SEQIDNO:82 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0001A954C8(BoNT/A.sub.1)sequencespanningactivationloop SEQIDNO:83 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI000016EA88(BoNT/A.sub.2)sequencespanningactivationloop SEQIDNO:84 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0000EF84BD(BoNT/A.sub.2)sequencespanningactivationloop SEQIDNO:85 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI000067C53E(BoNT/A.sub.2)sequencespanningactivationloop SEQIDNO:86 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI000290BDD5(BoNT/A.sub.2)sequencespanningactivationloop SEQIDNO:87 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0001C0B376(BoNT/A.sub.2)sequencespanningactivationloop SEQIDNO:88 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI000290BEB1(BoNT/A.sub.2)sequencespanningactivationloop SEQIDNO:89 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0001F3B30D(BoNT/A.sub.2)sequencespanningactivationloop SEQIDNO:90 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0001C32E84(BoNT/A.sub.2V2)sequencespanningactivationloop SEQIDNO:91 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UP10003C9D2A1(BoNT/A.sub.2or3)sequencespanningactivationloop SEQIDNO:92 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI000290B995(BoNT/A.sub.5,3or2)sequencespanningactivationloop SEQIDNO:93 TGLFEFYKLLCVRGIITSKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI00005B712C(BoNT/A.sub.3)sequencespanningactivationloop SEQIDNO:94 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNYLCIKVNNWDLFFSPSEDNFTNDL UPI00016DBC11(BoNT/A.sub.3)sequencespanningactivationloop SEQIDNO:95 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UP1000290C3D0(BoNT/A.sub.3)sequencespanningactivationloop SEQIDNO:96 TGLFEFYKLLCVRGIIPFKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI00005B712D(BoNT/A.sub.4)sequencespanningactivationloop SEQIDNO:97 TGLFEFYKLLCVRGIITSKTKSLDEGYNKALNELCIKVNNWDLFFSPSEDNFTNDL UPI00019DB885(BoNT/A.sub.4)sequencespanningactivationloop SEQIDNO:98 TGLFEFYKLLCVRGIITSKTKSLDEGYNKALNELCIKVNNWDLFFSPSEDNFTNDL UPI0001AE7D6A(BoNT/A.sub.5)sequencespanningactivationloop SEQIDNO:99 TGLFEFYKLLCVRGIITSKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI000198BDAE(BoNT/A.sub.5)sequencespanningactivationloop SEQIDNO:100 TGLFEFYKLLCVRGIITSKTKSLDEGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI0001B7D251(BoNT/A.sub.6)sequencespanningactivationloop SEQIDNO:101 TGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDL UPI00000B66D1(BoNT/F.sub.1)sequencespanningactivationloop SEQIDNO:102 KGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNRELFFVASESSYNEND UPI00016BB5D7(BoNT/F.sub.2)sequencespanningactivationloop SEQIDNO:103 KGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNNRELFFVASESSYNESD UPI00000B50F1(BoNT/F.sub.2)sequencespanningactivationloop SEQIDNO:104 KGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNNRELFFVASESSYNESD UPI0001C0B12F(BoNT/F.sub.3)sequencespanningactivationloop SEQIDNO:105 KGLVEKIIKFCKSIIPRKGTKQSPSLCIRVNNRELFFVASESSYNESD UPI0001A92658(BoNT/F.sub.4)sequencespanningactivationloop SEQIDNO:106 KGLVEKIVKLCKSIIPRKGTKAPPRLCIRVNNRELFFVASESSYNEND UPI0001C0B12D(BoNT/F.sub.4)sequencespanningactivationloop SEQIDNO:107 KGLVEKIVKLCKSIIPRKGTKAPPRLCIRVNNRELFFVASESSYNEND UPI0001C0B12E(BoNT/F.sub.5)sequencespanningactivationloop SEQIDNO:108 QGVVSRVVRLCLNSSFKKNTKKPLCITVNNGDLFFIASEDSYGEDT UPI0001C0B1D1(BoNT/F.sub.6)sequencespanningactivationloop SEQIDNO:109 KGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNSELFFVASESSYNEND UPI0000126B8A(BoNT/F.sub.6)sequencespanningactivationloop SEQIDNO:110 KGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNSELFFVASESSYNEND UPI0001C0B1D0(BoNT/F.sub.6)sequencespanningactivationloop SEQIDNO:111 KGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNSELFFVASESSYNEND UPI0001DE3DAC(BoNT/F.sub.7)sequencespanningactivationloop SEQIDNO:112 NGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASESSYNENG UPI00000BAF8C(BoNT/F.sub.7)sequencespanningactivationloop SEQIDNO:113 NGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASESSYNENG UPI0001C0B221(BoNT/F.sub.7)sequencespanningactivationloop SEQIDNO:114 NGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASESSYNENG UPI0001C0B220(BoNT/F.sub.7)sequencespanningactivationloop SEQIDNO:115 NGLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASESSYNENG BoNT/F.sub.7/.sub.1chimericnucleicacidsequencespanningactivationloop SEQIDNO:116 GGCCTGTGCAAAAGCGTTATTCCGCGTAAGGGTACGAAAGCACCGCCTCGCCTGTGCATTAAAGT BoNT/F.sub.7/.sub.1chimericaminoacidsequencespanningactivationloop SEQIDNO:117 GLCKSVIPRKGTKAPPRLCIKV BoNT/Fconsensusaminoacidsequencearoundactivationloop SEQIDNO:118 GLVEKIVKFCKSIIPRKGTKAPPRLCIRVNNRELFFVASES SXN102691F.sub.1aminoacidsequencearoundactivationloop SEQIDNO:119 GLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNRELFFVASES SXN102692F.sub.2aminoacidsequencearoundactivationloop SEQIDNO:120 GLVEKIIKFCKSIIPRKGTKQSPSLCIRVNNRELFFVASES SXN102693F.sub.3aminoacidsequencearoundactivationloop SEQIDNO:121 GLVEKIIKFCKSIIPRKGTKQSPSLCIRVNNRELFEVASES SXN102694F.sub.4aminoacidsequencearoundactivationloop SEQIDNO:122 GLVEKIVKLCKSIIPRKGTKAPPRLCIRVNNRELFFVASES SXN102695F.sub.5aminoacidsequencearoundactivationloop SEQIDNO:123 GVVSRVVRLCLNSSFKKNTKKPLCITVNNGDLFFIASED SXN102696F.sub.6aminoacidsequencearoundactivationloop SEQIDNO:124 GLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNSELFFVASES SXN102697F.sub.7aminoacidsequencearoundactivationloop SEQIDNO:125 GLVERFVGLCKSIVSKKGTKNSLCIKVNNRDLFFVASES Histag SEQIDNO:126 HHHHHH F.sub.1/.sub.4/.sub.5activationloopsequence(aftercleavagesite)+ startofF.sub.7heavy chainsequence SEQIDNO:127 APPR F.sub.2/.sub.3activationloopsequence(aftercleavagesite)+ startofF.sub.5heavy chainsequence SEQIDNO:128 QSPS F.sub.71forwardprimer SEQIDNO:129 CcaagAAGGGTACGAAAGCACCGCCTCGCCTGTGCATTAAAGTCAAC F.sub.71reverseprimer SEQIDNO:130 gtTgactTtaAtgcaCagGcgaGgcggTgCtTtCgtaccCttcttgg F.sub.72forwardprimer SEQIDNO:131 CtTcGtgggcctgtgcAaaagcgTTattCcgcgtaagggtAcgaaAgCaC F.sub.72reverseprimer SEQIDNO:132 gtgcTTtcgtaCCcTtacgcGgAatAacgcTTTtgcacaGgCCcacgAag