Filtration enhancement device for a circulating water culture system and method of making/maintaining same

Abstract

A filter enhancement device applied to a circulating water culture system and the method of making same, the device includes four components: protective shell, protective agents, microbial powder and proliferation promoting agents. The interior of the protective shell contains protective agents, microbial powder and proliferation promoting agents. Microbial powder and proliferation promoting agents are embedded in the modified-release layer.

Claims

1. A filtration enhancement device in a circulating water culture system, comprising the following components: microbial powder and proliferation promoting agents are mixed thoroughly according to a mass ratio of 1:110000, producing a microbial powder mixture; wherein the microbial powder mixture is embedded in timed-release layers of different thicknesses according to an expected release time, wherein the timed-release layers contains one or more layers, and the thickness of each layer is 1 to 1000 m; proliferation promoting agents, wherein the proliferation promoting agents are embedded in timed-release layers of different thicknesses, and the thickness of each timed-release layer is 1 to 1000 m; protective agents; and protective shell, wherein the embedded microbial powder mixture and proliferation promoting agents are within the protective shell containing the protective agents, according to the a mass ratio of 1:1-10.

2. The device of claim 1, wherein the protective agents are enclosed in the interior of the protective shell with the thickness range from 2 to 2000 m.

3. The device of claim 1, wherein the microbial powder is made of aerobic bacteria, facultative anaerobic bacteria, or anaerobic bacteria.

4. The device of claim 1, wherein the protective agents comprise any one or a combination selected from a group of sodium carboxymethyl cellulose, xanthan gum, shellac gum, sodium alginate, maltodextrin, starch, and soy protein.

5. The device of claim 1, wherein the materials of the protective shell comprises filtering papers, fabrics, or mesh filters.

6. The device of claim 1, wherein the proliferation promoting agents comprise any one or a combination selected from a group of skim milk, lactose, yeast extract, peptone, maltose, glucose, xylooligosaccharides, and fructooligosaccharides.

7. The device of claim 1, wherein materials used in the timed-release layers comprise any one or a combination selected from a group of sodium carboxymethylcellulose, xanthan gum, shellac gum, sodium alginate, maltodextrin, and modified starch.

8. The device of claim 1, wherein the device comprises a fixing component having a hole that can be affixed or suspended to a specific location in the circulating water culture system with self-adhesives.

9. A filtration device applied to a circulating water culture system with the device of claim 1 therein.

10. The device of claim 9, wherein a composition of microbial powder of the device is selected according to locations of deployment; aerobic bacteria are a main composition in anterior segments of the device; facultative anaerobic bacteria are a main composition in middle segments of the device; anaerobic bacteria are a main composition in posterior segments of the device.

11. A method for preparing a filtration enhancement device in a circulating water culture system, wherein the device comprises protective shell, protective agents, microbial powder, and proliferation promoting agents, wherein the method comprises: 1) mixing the microbial powder and proliferation promoting agents thoroughly according to a mass ratio of 1:110000, producing microbial powder mixture; 2) embedding the microbial powder mixture in timed-release layers of different thicknesses according to expected release time; wherein the thickness of each layer is 1 to 1000 m; 3) embedding the proliferation promoting agents in the timed-release layers; 4) adding the embedded microbial powder mixture and proliferation promoting agents according to a mass ratio of 1:1-10 to the protective shell containing protective agents, producing the filtration enhancement device circulating water culture system.

12. The method of claim 11, wherein the protective agents are enclosed in an interior of the protective shell with a thickness range from 2 to 2000 m.

13. The method of claim 11, wherein the microbial powder is made of aerobic bacteria, facultative anaerobic bacteria, or anaerobic bacteria.

14. The method of claim 11, wherein the protective agents comprise any one or a combination selected from a group of sodium carboxymethyl cellulose, xanthan gum, shellac gum, sodium alginate, maltodextrin, starch, and soy protein.

15. The method of claim 11, wherein the materials of the protective shell comprises filtering papers, fabrics, or mesh filters.

16. The method of claim 11, wherein the proliferation promoting agents comprise any one or a combination selected from a group of skim milk, lactose, yeast extract, peptone, maltose, glucose, xylooligosaccharides, and fructooligosaccharides.

17. The method of claim 11, wherein materials used in the timed-release layers comprise any one or a combination selected from a group of sodium carboxymethylcellulose, xanthan gum, shellac gum, sodium alginate, maltodextrin, and modified starch.

18. The method of claim 11, wherein the device comprises a fixing component having a hole that can be affixed or suspended to a specific location in the recirculating water culture system with self-adhesives.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIGS. 1 to 4 are various product diagrams of the filtration enhancement device in a circulating water culture system of the present disclosure. 1 is a fixing component, 2 is an extension cord and 3 is a protective shell;

(2) FIG. 5 is a comparison diagram of the filtration enhancement device in a circulating water culture system of the present disclosure before and after use;

(3) FIG. 6 is a diagram of each component in the filtration enhancement device in a circulating water culture system of the present disclosure. 3 is a protective shell, 4 (small black dot) is protective agents, 5 (triangle) is proliferation promoting agents, 6 (square) is microbial powder, and 7 (grey) is timed-release layer.

(4) FIG. 7 is the concentrations of ammonia nitrogen change during experiment;

(5) FIG. 8 is the concentrations of nitrite change during experiment;

(6) FIG. 9 is the concentrations of nitrate change during experiment.

DESCRIPTION OF EMBODIMENTS

(7) The following embodiments made from the above-stated materials, unless otherwise specified, are produced with conventional techniques and methods in the related fields. According to the described manufacturing techniques of the disclosure, further expansion of the production scale is feasible if combined with the basic knowledge of the related fields.

Embodiment 1

(8) The filtration enhancement device applied to a recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(9) Specifically, the microbial powder and proliferation promoting agents are mixed thoroughly according to mass ratio of 1:110000, producing microbial powder mixture; wherein the microbial powder mixture is embedded in timed-release layers of different thickness according to expected release time, wherein the timed-release layer contains one or more layers, and the thickness of each layer is 1 to 1000 m;

(10) Specifically, the proliferation promoting agents are embedded in timed-release layers of different thickness, the timed-release layer contains one or more layers, and the thickness of each layer is 1 to 1000 m;

(11) Specifically, the embedded microbial powder mixture and proliferation promoting agents are added to the protective shell containing protective agents, according to the mass ratio of 1:1-10, producing the filtration enhancement device in a circulating water culture system.

(12) The protective shell comprises a shell.

(13) The filtration enhancement device may further comprises an extension cord attached to the protective shell, and a fixing component attached to the extension cord.

(14) The protective shell is made of filtering papers.

(15) The fixing component contains a hole and self-adhesives.

(16) The protective agents are sodium carboxymethyl cellulose and xanthan gum.

(17) The microbial powder includes the Bacillus, the Actinomyces, nitrifying bacteria, nitrosated bacteria, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria.

(18) The proliferation promoting agents are skim milk and lactose.

(19) The timed-release layer is made of shellac gum and sodium alginate.

(20) The method for preparing the above-stated filtration enhancement device in a circulating water culture system comprises the below steps:

(21) 1) Mix thoroughly the microbial powder of the Bacillus, the Actinomycetes, nitrifying bacteria, nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of photosynthetic bacteria and denitrifying bacteria, producing anaerobic composite microbial powder bacteria.

(22) 2) Mix the proliferation promoting agents (skim milk and lactose in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(23) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into three portions respectively, and sequentially embed in shellac gum and sodium alginate. There are three embedding timed-release layers, the thicknesses of which are 3 m, 30 m and 300 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property.

(24) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (shellac gum and sodium alginate in a mass ration of 1:1), producing the filtration enhancement device in a circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(25) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in circulating water culture system.

Embodiment 2

(26) The filtration enhancement device applied to a recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(27) The protective shell comprises a shell, an extension cord is attached to the protective shell, and a fixing component is attached to the extension cord.

(28) The protective shell is made of fabrics.

(29) The fixing component contains a hole and self-adhesives.

(30) The protective agents are shellac gum and sodium alginate.

(31) The microbial powder includes the Actinomyces, yeast, nitrifying bacteria, nitrosated bacteria, lactic acid bacteria and photosynthetic bacteria.

(32) The proliferation promoting agents are yeast extract and peptone.

(33) The timed-release layer is made of maltodextrin and modified starch.

(34) The method for preparing the above-stated filtration enhancement device in a circulating water culture system comprises the below steps:

(35) 1) Mix thoroughly the microbial powder of the Bacillus, the Actinomycetes, yeasts, nitrifying bacteria, nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of photosynthetic bacteria, producing anaerobic composite microbial powder bacteria.

(36) 2) Mix the proliferation promoting agents (yeast extract and peptone in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(37) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into three portions respectively, and sequentially embed in maltodextrin and modified starch. There are three embedding layers, the thicknesses of which are 2 m, 30 m and 250 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property

(38) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (shellac gum and sodium alginate in a mass ration of 1:1), producing the filtration enhancement device in circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(39) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in circulating water culture system.

Embodiment 3

(40) The filtration enhancement device applied to recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(41) The protective shell comprises a shell, an extension cord is attached to the protective shell, and a fixing component is attached to the extension cord.

(42) The protective shell is made of mesh filters.

(43) The fixing component contains a hole.

(44) The protective agents are maltodextrin and starch.

(45) The microbial powder includes the Bacillus, yeasts, nitrifying bacteria, nitrification bacteria, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria.

(46) The proliferation promoting agents are maltose and glucose.

(47) The timed-release layer is made of magnesium stearate and sodium carboxymethyl cellulose.

(48) The method for preparing the above-stated filtration enhancement device in circulating water culture system comprises the below steps:

(49) 1) Mix thoroughly the microbial powder of the Bacillus, nitrifying bacteria and nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of photosynthetic bacteria and denitrifying bacteria, producing anaerobic composite microbial powder bacteria.

(50) 2) Mix the proliferation promoting agents (maltose and glucose in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(51) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into three portions respectively, and sequentially embed in magnesium stearate and sodium carboxymethyl cellulose. There are three embedding layers, the thicknesses of which are 2 m, 40 m and 200 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property

(52) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (maltodextrin and starch in a mass ration of 1:1), producing the filtration enhancement device in circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(53) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in circulating water culture system.

Embodiment 4

(54) The filtration enhancement device applied to recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(55) The protective shell comprises a shell, an extension cord is attached to the protective shell, and a fixing component is attached to the extension cord.

(56) The protective shell is made of filtering papers.

(57) The fixing component contains a hole and self-adhesives.

(58) The protective agents are soy protein and sodium alginate.

(59) The microbial powder includes the Bacillus, nitrifying bacteria, nitrosated bacteria, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria.

(60) The proliferation promoting agents are xylooligosaccharides and fructooligosaccharides.

(61) The timed-release layer is made of xanthan gum and shellac gum.

(62) The method for preparing the above-stated filtration enhancement device in circulating water culture system comprises the below steps:

(63) 1) Mix thoroughly the microbial powder of the Bacillus, nitrifying bacteria and nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of photosynthetic bacteria and denitrifying bacteria, producing anaerobic composite microbial powder bacteria.

(64) 2) Mix the proliferation promoting agents (xylooligosaccharides and fructooligosaccharides in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(65) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into three portions respectively, and sequentially embed in xanthan gum and shellac gum. There are three embedding layers, the thicknesses of which are 1 m, 30 m and 300 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property

(66) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (soy protein and sodium alginate in a mass ration of 1:1), producing the filtration enhancement device in a circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(67) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in circulating water culture system.

Embodiment 5

(68) The filtration enhancement device applied to recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(69) The protective shell comprises a shell, an extension cord is attached to the protective shell, and a fixing component is attached to the extension cord.

(70) The protective shell is made of fabrics

(71) The fixing component contains a hole and self-adhesives.

(72) The protective agents are sodium carboxymethyl cellulose and shellac gum.

(73) The microbial powder includes the Actinomyces, nitrifying bacteria, nitrosated bacteria, lactic acid bacteria and denitrifying bacteria.

(74) The proliferation promoting agents are skim milk and yeast extract.

(75) The timed-release layer is made of sodium alginate, maltodextrin and modified starch.

(76) The method for preparing the above-stated filtration enhancement device in circulating water culture system comprises the below steps:

(77) 1) Mix thoroughly the microbial powder of the Actinomycetes, nitrifying bacteria and nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of denitrifying bacteria, producing anaerobic composite microbial powder bacteria.

(78) 2) Mix the proliferation promoting agents (skim milk and yeast extract in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(79) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into two portions respectively, and sequentially embed in shellac gum and sodium alginate. There are two embedding layers, the thicknesses of which are 10 m and 100 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property

(80) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (sodium carboxymethyl cellulose and shellac gum in a mass ration of 1:1), producing the filtration enhancement device in a circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(81) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in circulating water culture system.

Embodiment 6

(82) The filtration enhancement device applied to a recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(83) The protective shell comprises a shell, an extension cord is attached to the protective shell, and a fixing component is attached to the extension cord.

(84) The protective shell is made of mesh filters.

(85) The fixing component contains a hole.

(86) The protective agents are xanthan gum and sodium alginate.

(87) The microbial powder includes yeast, nitrifying bacteria, nitrosated bacteria, lactic acid bacteria and denitrifying bacteria.

(88) The proliferation promoting agents are maltose and xylooligosaccharides.

(89) The timed-release layer is made of magnesium stearate, sodium carboxymethyl cellulose and shellac gum.

(90) The method for preparing the above-stated filtration enhancement device in circulating water culture system comprises the below steps:

(91) 1) Mix thoroughly the microbial powder of yeast, nitrifying bacteria and nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of denitrifying bacteria, producing anaerobic composite microbial powder bacteria.

(92) 2) Mix the proliferation promoting agents (maltose and xylooligosaccharides in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(93) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into four portions respectively, and sequentially embed in magnesium stearate, sodium carboxymethylcellulose and shellac gum. There are four embedding layers, the thicknesses of which are 2 m, 30 m, 100 m and 200 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property

(94) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (xanthan gum and sodium alginate in a mass ration of 1:1), producing the filtration enhancement device in a circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(95) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in circulating water culture system.

Embodiment 7

(96) The filtration enhancement device applied to a recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(97) The protective shell comprises a shell, an extension cord is attached to the protective shell, and a fixing component is attached to the extension cord.

(98) The protective shell is made of filtering papers.

(99) The fixing component contains a hole.

(100) The protective agents are maltodextrin and soy protein.

(101) The microbial powder includes the Bacillus, the Actinomyces, nitrifying bacteria, nitrosated bacteria, lactic acid bacteria and denitrifying bacteria.

(102) The proliferation promoting agents are lactose and peptone.

(103) The timed-release layer is made of xanthan gum and sodium alginate.

(104) The method for preparing the above-stated filtration enhancement device in circulating water culture system comprises the below steps:

(105) 1) Mix thoroughly the microbial powder of the Bacillus, the Actinomycetes, nitrifying bacteria, nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of denitrifying bacteria, producing anaerobic composite microbial powder bacteria.

(106) 2) Mix the proliferation promoting agents (lactose and peptone in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(107) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into two portions respectively, and sequentially embed in xanthan gum and sodium alginate. There are two embedding layers, the thicknesses of which are 10 m and 200 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property

(108) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (maltodextrin and soy protein in a mass ration of 1:1), producing the filtration enhancement device in a circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(109) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in circulating water culture system.

Embodiment 8

(110) The filtration enhancement device applied to a recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(111) The protective shell comprises a shell, an extension cord is attached to the protective shell, and a fixing component is attached to the extension cord.

(112) The protective shell is made of fabrics.

(113) The fixing component contains a hole and self-adhesives.

(114) The protective agents are starch and sodium alginate.

(115) The microbial powder includes the Bacillus, the Actinomyces, yeast, nitrifying bacteria, nitrosated bacteria, lactic acid bacteria and photosynthetic bacteria.

(116) The proliferation promoting agents are glucose and fructooligosaccharides.

(117) The timed-release layer is made of shellac gum and maltodextrin.

(118) The method for preparing the above-stated filtration enhancement device in circulating water culture system comprises the below steps:

(119) 1) Mix thoroughly the microbial powder of the Bacillus, the Actinomycetes, yeasts, nitrifying bacteria, nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of photosynthetic bacteria, producing anaerobic composite microbial powder bacteria.

(120) 2) Mix the proliferation promoting agents (glucose and fructooligosaccharides in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(121) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into three portions respectively, and sequentially embed in shellac gum and maltodextrin. There are three embedding layers, the thicknesses of which are 2 m, 50 m and 200 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property

(122) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (starch and sodium alginate in a mass ration of 1:1), producing the filtration enhancement device in a circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(123) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in circulating water culture system.

Embodiment 9

(124) The filtration enhancement device applied to a recirculating aquaculture system includes four components: protective shell, protective agents, microbial powder, and proliferation promoting agents.

(125) The protective shell comprises a shell, an extension cord is attached to the protective shell, and a fixing component is attached to the extension cord.

(126) The protective shell is made of mesh filters.

(127) The fixing component contains a hole and self-adhesives.

(128) The protective agents are sodium carboxymethyl cellulose and starch.

(129) The microbial powder includes the Actinomyces, yeast, nitrifying bacteria, nitrosated bacteria, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria.

(130) The proliferation promoting agents are skim milk and peptone.

(131) The timed-release layer is made of sodium alginate and modified starch.

(132) The method for preparing the above-stated filtration enhancement device in circulating water culture system comprises the below steps:

(133) 1) Mix thoroughly the microbial powder of the Actinomycetes, yeast, nitrifying bacteria, nitrosated bacteria, producing aerobic composite microbial powder; mix thoroughly the microbial powder of lactic acid bacteria, producing facultative anaerobic composite microbial powder; mix thoroughly the microbial powder of photosynthetic bacteria and denitrifying bacteria, producing anaerobic composite microbial powder bacteria.

(134) 2) Mix the proliferation promoting agents (skim milk and peptone in a mass ration of 1:1) thoroughly and respectively with the composite microbial powder characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(135) 3) Divide the mixture from step 2) and the proliferation promoting agents alone into three portions respectively, and sequentially embed in sodium alginate and modified starch. There are three embedding layers, the thicknesses of which are 1 m, 60 m and 180 m respectively, producing respectively aerobic composite microbial agents, facultative anaerobic composite microbial agents, anaerobic composite microbial agents and proliferation promoting agents with modified-release property

(136) 4) Add the composite microbial agents and proliferation promoting agents from step 3) to the protective shell containing protective agents (sodium carboxymethyl cellulose and starch in a mass ration of 1:1), producing the filtration enhancement device in circulating water culture system characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria.

(137) 5) Fix the filtration enhancement device characterizing in aerobic bacteria, facultative anaerobic bacteria or anaerobic bacteria to their corresponding locations according to the internal circumstance of the filter in a circulating water culture system.

(138) In order to test the effect of the filtration enhancement device of the present disclosure on the filtration system in a circulating water culture system, the following experiment was conducted.

(139) Five groups of aquarium with filters are selected. Each aquarium keeps three healthy koi with the body length ranged from 20 to 25 centimeters, which are provided with the same amount of feeds daily. The five experiment groups are set as the following treatments respectively:

(140) Experiment Group 1: no treatment was applied;

(141) Experiment Group 2: the filtration enhancement device of Embodiment 1 was applied;

(142) Experiment Group 3: the commercial powdered microbial agents for water purification was applied.

(143) Experiment Group 4: the commercial liquid microbial agents for water purification was applied.

(144) Experiment Group 5: the commercial microbial agents for water purification with modified-release property was applied;

(145) The experiment results demonstrated that in Experimental Group 1, the concentrations of ammonia nitrogen, and nitrate continued to increase in water during the experiment. In Experimental Group 2, 3, 4 and 5, the concentrations of ammonia nitrogen, nitrite and nitrate dropped relative to Experiment Group 1. However, as time passed by and water conditions diluted the microbial agents, the concentrations of the pollutants began to rise slowly, indicating that the lifespans of such products are shorter and need to be repeatedly applied, which not only causes inconvenience but also increased maintenance costs. Though the product in Experimental Group 5 featured some modified-release properties, which made its purification capacity better than those of Experimental Group 3 and 4, the concentrations of ammonia nitrogen, nitrite and nitrate still rose after 30 days due to the dilution effect of the water and reapplication was required. In Experimental group 2, the water purification capacity remained at a high level throughout the experiment and the concentrations of ammonia nitrogen, nitrite and nitrate did not increase in any meaningful way nor to dangerous levels. Therefore, no further action, on the part of the user, is required over an extended period of time, which provides a great convenience, reduced expense and less waste or recyclable material.

REFERENCES CITED

U.S. Patent Documents

(146) U.S. Pat. No. 5,011,679 A April 1991 Spanier et al.

Foreign Patents or Applications

(147) 101264985B July 2011 China Guo et al.

(148) 206232700U June 2017 China Lv et al.