URACYL SPIROOXETANE NUCLEOSIDES

Abstract

The present invention relates to compounds of the formula I:

##STR00001##

including any possible stereoisomers thereof, wherein R.sup.9 has the meaning as defined herein, or a pharmaceutically acceptable salt or solvate thereof

The present invention also relates to processes for preparing said compounds, pharmaceutical compositions containing them and their use, alone or in combination with other HCV inhibitors, in HCV therapy.

Claims

1-5. (canceled)

6. A compound of formula Ib: ##STR00011##

7-9. (canceled)

10. A pharmaceutical composition comprising a compound according to claim 6, and a pharmaceutically acceptable carrier.

11-13. (canceled)

14. A method of preparing a compound of formula (7), ##STR00012## comprising reacting a compound of formula (12) with POCl.sub.3 to provide a compound of formula (6); reacting a compound of formula (6) with a compound of formula (4), wherein R.sup.9 is selected from the group consisting of C.sub.1-C.sub.6alkyl, phenyl, naphtyl, C.sub.3-C.sub.7cycloalkyl, and Y, wherein Y is C.sub.1-C.sub.3alkyl substituted with 1, 2 or 3 substituents each independently selected from the group consisting of phenyl, C.sub.3-C.sub.6cycloalkyl, hydroxy and C.sub.1-C.sub.6alkoxy.

15. A method as claimed in claim 14, wherein said reacting a compound of formula (6) with a compound of formula (4) takes place in the presence of triethylamine.

16. A method as claimed in claim 14, wherein said reacting a compound of formula (6) with a compound of formula (4) takes place in the presence of N-methylimidazole.

17. A method as claimed in claim 14, wherein said reacting a compound of formula (6) with a compound of formula (4) takes place in dichloromethane.

18. A method as claimed in claim 14, wherein said reacting a compound of formula (6) with a compound of formula (4) takes place in the presence of triethylamine and N-methylimidazole in dichloromethane.

19. A method as claimed in claim 14, wherein R.sup.9 is C.sub.1-C.sub.6alkyl.

20. A method as claimed in claim 14, wherein R.sup.9 is C.sub.3-C.sub.7cycloalkyl.

21. A method as claimed in claim 14, wherein R.sup.9 is isopropyl.

22. A method as claimed in claim 14, wherein R.sup.9 is C.sub.2-C.sub.4alkyl.

23. A method as claimed in claim 14, wherein R.sup.9 is C.sub.1-C.sub.2alkyl substituted with phenyl.

Description

SHORT DESCRIPTION OF THE FIGURE

[0039] FIG. 1: In vivo efficacy of compound 8a and CAS-1375074-52-4 as determined in a humanized hepatocyte mouse model.

DEFINITIONS

[0040] As used herein C.sub.1-C.sub.nalkyl as a group or part of a group defines saturated straight or branched chain hydrocarbon radicals having from 1 to n carbon atoms. Accordingly, C.sub.1-C.sub.4alkyl as a group or part of a group defines saturated straight or branched chain hydrocarbon radicals having from 1 to 4 carbon atoms such as for example methyl, ethyl, 1-propyl, 2-propyl, 1-butyl, 2-butyl, 2-methyl-1-propyl, 2-methyl-2-propyl. C.sub.1-C.sub.6alkyl encompasses C.sub.1-C.sub.4alkyl radicals and the higher homologues thereof having 5 or 6 carbon atoms such as, for example, 1-pentyl, 2-pentyl, 3-pentyl, 1-hexyl, 2-hexyl, 2-methyl-1-butyl, 2-methyl-1-pentyl, 2-ethyl-1-butyl, 3-methyl-2-pentyl, and the like. Of interest amongst C.sub.1-C.sub.6alkyl is C.sub.1-C.sub.4alkyl.

[0041] C.sub.1-C.sub.nalkoxy means a radical OC.sub.1-C.sub.nalkyl wherein C.sub.1-C.sub.nalkyl is as defined above. Accordingly, C.sub.1-C.sub.6alkoxy means a radical OC.sub.1-C.sub.6alkyl wherein C.sub.1-C.sub.6alkyl is as defined above. Examples of C.sub.1-C.sub.6alkoxy are methoxy, ethoxy, n-propoxy, or isopropoxy. Of interest is C.sub.1-C.sub.2alkoxy, encompassing methoxy and ethoxy.

[0042] C.sub.3-C.sub.6cycloalkyl includes cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

[0043] In one embodiment, the term phenyl-C.sub.1-C.sub.6alkyl is benzyl.

[0044] As used herein, the term (O) or oxo forms a carbonyl moiety when attached to a carbon atom. It should be noted that an atom can only be substituted with an oxo group when the valency of that atom so permits.

[0045] The term monophosphate, diphosphate or triphosphate ester refers to groups:

##STR00007##

[0046] Where the position of a radical on a molecular moiety is not specified (for example a substituent on phenyl) or is represented by a floating bond, such radical may be positioned on any atom of such a moiety, as long as the resulting structure is chemically stable. When any variable is present more than once in the molecule, each definition is independent.

[0047] Whenever used herein, the term compounds of formula I, or the present compounds or similar terms, it is meant to include the compounds of Formula I, Ia and Ib, including the possible stereochemically isomeric forms, and their pharmaceutically acceptable salts and solvates.

[0048] The present invention also includes isotope-labeled compounds of formula I or any subgroup of formula I, wherein one or more of the atoms is replaced by an isotope that differs from the one(s) typically found in nature. Examples of such isotopes include isotopes of hydrogen, such as .sup.2H and .sup.3H; carbon, such as .sup.11C, .sup.13C and .sup.14C; nitrogen, such as .sup.13N and .sup.15N; oxygen, such as .sup.15O, .sup.17O and .sup.18O; phosphorus, such as .sup.31P and .sup.32P, sulphur, such as .sup.35S; fluorine, such as .sup.18P; chlorine, such as .sup.36C.sub.1; bromine such as .sup.75Br, .sup.76Br, .sup.77Br and .sup.82Br; and iodine, such as .sup.123I, .sup.124I, .sup.125I and .sup.131I. Isotope-labeled compounds of the invention can be prepared by processes analogous to those described herein by using the appropriate isotope-labeled reagents or starting materials, or by art-known techniques. The choice of the isotope included in an isotope-labeled compound depends on the specific application of that compound. For example, for tissue distribution assays, a radioactive isotope such as .sup.3H or .sup.14C is incorporated. For radio-imaging applications, a positron emitting isotope such as .sup.11C, .sup.18F, .sup.13N or .sup.15O will be useful. The incorporation of deuterium may provide greater metabolic stability, resulting in, e.g. an increased in vivo half life of the compound or reduced dosage requirements.

[0049] General Synthetic Procedures

[0050] The following schemes are just meant to be illustrative and are by no means limiting the scope.

[0051] The starting material 1-[(4R,5R,7R,8R)-8-hydroxy-7-(hydroxymethyl)-1,6-dioxaspiro[3.4]octan-5-yl]pyrimidine-2,4(1H,3H)-dione (1) can be prepared as exemplified in WO2010/130726. Compound (1) is converted into compounds of the present invention via a p-methoxybenzyl protected derivative (4) as exemplified in the following Scheme 1.

##STR00008##

[0052] In Scheme 1, R.sup.9 can be C.sub.1-C.sub.6alkyl, phenyl, naphtyl, C.sub.3-C.sub.7cycloalkyl or C.sub.1-C.sub.3alkyl substituted with 1, 2 or 3 substituents each independently selected from phenyl, C.sub.3-C.sub.6cycloalkyl, hydroxy, or C.sub.1-C.sub.6alkoxy, preferably R.sup.9 is C.sub.1-C.sub.6alkyl or C.sub.1-C.sub.2alkyl substituted with phenyl, C.sub.1-C.sub.2alkoxy or C.sub.3-C.sub.6cycloalkyl, even more preferably R.sup.9 is C.sub.2-C.sub.4alkyl and most preferably R.sup.9 is i-propyl.

[0053] In a further aspect, the present invention concerns a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula I as specified herein, and a pharmaceutically acceptable carrier. Said composition may contain from 1% to 50%, or from 10% to 40% of a compound of formula I and the remainder of the composition is the said carrier. A therapeutically effective amount in this context is an amount sufficient to act in a prophylactic way against HCV infection, to inhibit HCV, to stabilize or to reduce HCV infection, in infected subjects or subjects being at risk of becoming infected. In still a further aspect, this invention relates to a process of preparing a pharmaceutical composition as specified herein, which comprises intimately mixing a pharmaceutically acceptable carrier with a therapeutically effective amount of a compound of formula I, as specified herein.

[0054] The compounds of formula I or of any subgroup thereof may be formulated into various pharmaceutical forms for administration purposes. As appropriate compositions there may be cited all compositions usually employed for systemically administering drugs. To prepare the pharmaceutical compositions of this invention, an effective amount of the particular compound, optionally in addition salt form or metal complex, as the active ingredient is combined in intimate admixture with a pharmaceutically acceptable carrier, which carrier may take a wide variety of forms depending on the form of preparation desired for administration. These pharmaceutical compositions are desirable in unitary dosage form suitable, particularly, for administration orally, rectally, percutaneously, or by parenteral injection. For example, in preparing the compositions in oral dosage form, any of the usual pharmaceutical media may be employed such as, for example, water, glycols, oils, alcohols and the like in the case of oral liquid preparations such as suspensions, syrups, elixirs, emulsions and solutions; or solid carriers such as starches, sugars, kaolin, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules, and tablets. Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage unit forms, in which case solid pharmaceutical carriers are obviously employed. For parenteral compositions, the carrier will usually comprise sterile water, at least in large part, though other ingredients, for example, to aid solubility, may be included. Injectable solutions, for example, may be prepared in which the carrier comprises saline solution, glucose solution or a mixture of saline and glucose solution. Injectable suspensions may also be prepared in which case appropriate liquid carriers, suspending agents and the like may be employed. Also included are solid form preparations intended to be converted, shortly before use, to liquid form preparations. In the compositions suitable for percutaneous administration, the carrier optionally comprises a penetration enhancing agent and/or a suitable wetting agent, optionally combined with suitable additives of any nature in minor proportions, which additives do not introduce a significant deleterious effect on the skin. The compounds of the present invention may also be administered via oral inhalation or insufflation in the form of a solution, a suspension or a dry powder using any art-known delivery system.

[0055] It is especially advantageous to formulate the aforementioned pharmaceutical compositions in unit dosage form for ease of administration and uniformity of dosage. Unit dosage form as used herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Examples of such unit dosage forms are tablets (including scored or coated tablets), capsules, pills, suppositories, powder packets, wafers, injectable solutions or suspensions and the like, and segregated multiples thereof.

[0056] The compounds of formula I show activity against HCV and can be used in the treatment and/or prophylaxis of HCV infection or diseases associated with HCV. The latter include progressive liver fibrosis, inflammation and necrosis leading to cirrhosis, end-stage liver disease, and HCC. The compounds of this invention moreover are believed to be active against mutated strains of HCV and show a favorable pharmacokinetic profile and have attractive properties in terms of bioavailability, including an acceptable half-life, AUC (area under the curve) and peak values and lacking unfavorable phenomena such as insufficient quick onset and tissue retention.

[0057] The in vitro antiviral activity against HCV of the compounds of formula I can be tested in a cellular HCV replicon system based on Lohmann et al. (1999) Science 285:110-113, with the further modifications described by Krieger et al. (2001) Journal of Virology 75: 4614-4624 (incorporated herein by reference), which is further exemplified in the examples section. This model, while not a complete infection model for HCV, is widely accepted as the most robust and efficient model of autonomous HCV RNA replication currently available. It will be appreciated that it is important to distinguish between compounds that specifically interfere with HCV functions from those that exert cytotoxic or cytostatic effects in the HCV replicon model, and as a consequence cause a decrease in HCV RNA or linked reporter enzyme concentration. Assays are known in the field for the evaluation of cellular cytotoxicity based for example on the activity of mitochondrial enzymes using fluorogenic redox dyes such as resazurin. Furthermore, cellular counter screens exist for the evaluation of non-selective inhibition of linked reporter gene activity, such as firefly luciferase. Appropriate cell types can be equipped by stable transfection with a luciferase reporter gene whose expression is dependent on a constitutively active gene promoter, and such cells can be used as a counter-screen to eliminate non-selective inhibitors.

[0058] Due to their anti-HCV properties, the compounds of formula I, including any possible stereoisomers, the pharmaceutically acceptable addition salts or solvates thereof, are useful in the treatment of warm-blooded animals, in particular humans, infected with HCV, and in the prophylaxis of HCV infections. The compounds of the present invention may therefore be used as a medicine, in particular as an anti-HCV or a HCV-inhibiting medicine. The present invention also relates to the use of the present compounds in the manufacture of a medicament for the treatment or the prevention of HCV infection. In a further aspect, the present invention relates to a method of treating a warm-blooded animal, in particular human, infected by HCV, or being at risk of becoming infected by HCV, said method comprising the administration of an anti-HCV effective amount of a compound of formula I, as specified herein. Said use as a medicine or method of treatment comprises the systemic administration to HCV-infected subjects or to subjects susceptible to HCV infection of an amount effective to combat the conditions associated with HCV infection.

[0059] In general it is contemplated that an antiviral effective daily amount would be from about 1 to about 30 mg/kg, or about 2 to about 25 mg/kg, or about 5 to about 15 mg/kg, or about 8 to about 12 mg/kg body weight. Average daily doses can be obtained by multiplying these daily amounts by about 70. It may be appropriate to administer the required dose as two, three, four or more sub-doses at appropriate intervals throughout the day. Said sub-doses may be formulated as unit dosage forms, for example, containing about 1 to about 2000 mg, or about 50 to about 1500 mg, or about 100 to about 1000 mg, or about 150 to about 600 mg, or about 100 to about 400 mg of active ingredient per unit dosage form.

[0060] As used herein the term about has the meaning known to the person skilled in the art. In certain embodiments the term about may be left out and the exact amount is meant. In other embodiments the term about means that the numerical following the term about is in the range of 15%, or of 10%, or of 5%, or of 1%, of said numerical value.

Examples

[0061] ##STR00009##

[0062] Synthesis of Compound (2)

[0063] Compound (2) can be prepared by dissolving compound (1) in pyridine and adding 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane. The reaction is stirred at room temperature until complete. The solvent is removed and the product redissolved in CH.sub.2Cl.sub.2 and washed with saturated NaHCO.sub.3 solution. Drying on MgSO.sub.4 and removal of the solvent gives compound (2).

[0064] Synthesis of Compound (3)

[0065] Compound (3) is prepared by reacting compound (2) with p-methoxybenzylchloride in the presence of DBU as the base in CH.sub.3CN.

[0066] Synthesis of Compound (4)

[0067] Compound (4) is prepared by cleavage of the bis-silyl protecting group in compound (3) using TBAF as the fluoride source.

[0068] Synthesis of Compound (6a)

[0069] A solution of isopropyl alcohol (3.86 mL, 0.05 mol) and triethylamine (6.983 mL, 0.05 mol) in dichloromethane (50 mL) was added to a stirred solution of POCl.sub.3 (5) (5.0 mL, 0.0551 mol) in DCM (50 mL) dropwise over a period of 25 min at 5 C. After the mixture stirred for 1h, the solvent was evaporated, and the residue was suspended in ether (100 mL). The triethylamine hydrochloride salt was filtered and washed with ether (20 mL). The filtrate was concentrated, and the residue was distilled to give the (6) as a colorless liquid (6.1 g, 69% yield).

[0070] Synthesis of Compound (7a)

[0071] To a stirred suspension of (4) (2.0 g, 5.13 mmol) in dichloromethane (50 mL) was added triethylamine (2.07 g, 20.46 mmol) at room temperature. The reaction mixture was cooled to 20 C., and then (6a) (1.2 g, 6.78 mmol) was added dropwise over a period of 10 min. The mixture was stirred at this temperature for 15 min and then NMI was added (0.84 g, 10.23 mmol), dropwise over a period of 15 min. The mixture was stirred at 15 C. for 1 h and then slowly warmed to room temperature in 20 h. The solvent was evaporated, the mixture was concentrated and purified by column chromatography using petroleum ether/EtOAc (10:1 to 5:1 as a gradient) to give (7a) as white solid (0.8 g, 32% yield).

[0072] Synthesis of Compound (8a)

[0073] To a solution of (7a) in CH.sub.3CN (30 mL) and H.sub.2O (7 mL) was add CAN portion wise below 20 C. The mixture was stirred at 15-20 C. for 5h under N.sub.2. Na.sub.2SO.sub.3 (370 mL) was added dropwise into the reaction mixture below 15 C., and then Na.sub.2CO.sub.3 (370 mL) was added. The mixture was filtered and the filtrate was extracted with CH.sub.2Cl.sub.2 (100 mL*3). The organic layer was dried and concentrated to give the residue. The residue was purified by column chromatography to give the target compound (8a) as white solid. (Yield: 55%)

[0074] .sup.1H NMR (400 MHz, CHLOROFORM-d) ppm 1.45 (dd, J=7.53, 6.27 Hz, 6H), 2.65-2.84 (m, 2H), 3.98 (td, J=10.29, 4.77 Hz, 1H), 4.27 (t, J=9.66 Hz, 1H), 4.43 (ddd, J=8.91, 5.77, 5.65 Hz, 1H), 4.49-4.61 (m, 1H), 4.65 (td, J=7.78, 5.77 Hz, 1H), 4.73 (d, J=7.78 Hz, 1H), 4.87 (dq, J=12.74, 6.30 Hz, 1H), 5.55 (br. s., 1H), 5.82 (d, J=8.03 Hz, 1H), 7.20 (d, J=8.03 Hz, 1H), 8.78 (br. s., 1H); .sup.31P NMR (CHLOROFORM-d) ppm 7.13; LC-MS: 375 (M+1)+

##STR00010##

[0075] Step 1: Synthesis of Compound (9)

[0076] Compound (1), CAS 1255860-33-3 (1200 mg, 4.33 mmol) and 1,8-bis(dimethyl-amino)naphthalene (3707 mg, 17.3 mmol) were dissolved in 24.3 mL of trimethylphosphate. The solution was cooled to 0 C. Compound (5) (1.21 mL, 12.98 mmol) was added, and the mixture was stirred well maintaining the temperature at 0 C. for 5 hours. The reaction was quenched by addition of 120 mL of tetraethyl-ammonium bromide solution (1M) and extracted with CH.sub.2Cl.sub.2 (280 mL). Purification was done by preparative HPLC (Stationary phase: RP XBridge Prep C18 OBD-10 m, 30150 mm, mobile phase: 0.25% NH.sub.4HCO.sub.3 solution in water, CH.sub.3CN), yielding two fractions. The purest fraction was dissolved in water (15 mL) and passed through a manually packed Dowex (H.sup.+) column by elution with water. The end of the elution was determined by checking UV absorbance of eluting fractions. Combined fractions were frozen at 78 C. and lyophilized. Compound (9) was obtained as a white fluffy solid (303 mg, (0.86 mmol, 20% yield), which was used immediately in the following reaction.

[0077] Step 2: Preparation of Compound (VI)

[0078] Compound (9) (303 mg, 0.86 mmol) was dissolved in 8 mL water and to this solution was added N,N-Dicyclohexyl-4-morpholine carboxamidine (253.8 mg, 0.86 mmol) dissolved in pyridine (8.4 mL). The mixture was kept for 5 minutes and then evaporated to dryness, dried overnight in vacuo overnight at 37 C. The residue was dissolved in pyridine (80 mL). This solution was added dropwise to vigorously stirred DCC (892.6 mg, 4.326 mmol) in pyridine (80 mL) at reflux temperature. The solution was kept refluxing for 1.5h during which some turbidity was observed in the solution. The reaction mixture was cooled and evaporated to dryness. Diethylether (50 mL) and water (50 mL) were added to the solid residue. NN-dicyclohexylurea was filtered off, and the aqueous fraction was purified by preparative HPLC (Stationary phase: RP XBridge Prep C18 OBD-10 m, 30150 mm, mobile phase: 0.25% NH.sub.4HCO.sub.3 solution in water, CH.sub.3CN), yielding a white solid which was dried overnight in vacuo at 38 C. (185 mg, 0.56 mmol, 65% yield). LC-MS: (M+H).sup.+: 333.

[0079] .sup.1H NMR (400 MHz, DMSO-d.sub.6) ppm 2.44-2.59 (m, 2H) signal falls under DMSO signal, 3.51 (td, J=9.90, 5.50 Hz, 1H), 3.95-4.11 (m, 2H), 4.16 (d, J=10.34 Hz, 1H), 4.25-4.40 (m, 2H), 5.65 (d, J=8.14 Hz, 1H), 5.93 (br. s., 1H), 7.46 (d, J=7.92 Hz, 1H), 2H's not observed

Biological Examples

[0080] Replicon Assays

[0081] The compounds of formula I were examined for activity in the inhibition of HCV-RNA replication in a cellular assay. The assay was used to demonstrate that the compounds of formula I inhibited a HCV functional cellular replicating cell line, also known as HCV replicons. The cellular assay was based on a bicistronic expression construct, as described by Lohmann et al. (1999) Science vol. 285 pp. 110-113 with modifications described by Krieger et al. (2001) Journal of Virology 75: 4614-4624, in a multi-target screening strategy.

[0082] Replicon Assay (A)

[0083] In essence, the method was as follows. The assay utilized the stably transfected cell line Huh-7 luc/neo (hereafter referred to as Huh-Luc). This cell line harbors an RNA encoding a bicistronic expression construct comprising the wild type NS3-NS5B regions of HCV type 1b translated from an internal ribosome entry site (IRES) from encephalomyocarditis virus (EMCV), preceded by a reporter portion (FfL-luciferase), and a selectable marker portion (neo.sup.R, neomycine phosphotransferase). The construct is bordered by 5 and 3 NTRs (non-translated regions) from HCV genotype 1b.

[0084] Continued culture of the replicon cells in the presence of G418 (neo.sup.R) is dependent on the replication of the HCV-RNA. The stably transfected replicon cells that express HCV-RNA, which replicates autonomously and to high levels, encoding inter alia luciferase, were used for screening the antiviral compounds.

[0085] The replicon cells were plated in 384-well plates in the presence of the test and control compounds which were added in various concentrations. Following an incubation of three days, HCV replication was measured by assaying luciferase activity (using standard luciferase assay substrates and reagents and a Perkin Elmer ViewLux ultraHTS microplate imager). Replicon cells in the control cultures have high luciferase expression in the absence of any inhibitor. The inhibitory activity of the compound on luciferase activity was monitored on the Huh-Luc cells, enabling a dose-response curve for each test compound. EC.sub.50 values were then calculated, which value represents the amount of the compound required to decrease the level of detected luciferase activity by 50%, or more specifically, the ability of the genetically linked HCV replicon RNA to replicate.

[0086] Results (A)

[0087] Table 1 shows the replicon results (EC.sub.50, replicon) and cytotoxicity results (CC.sub.50 (M) (Huh-7)) obtained for the compound of the examples given above.

TABLE-US-00001 TABLE 1 Compound CC.sub.50 (M) number EC.sub.50 (M) (HCV) (Huh-7) 8a 0.13 (n = 4) >100

[0088] Replicon Assays (B)

[0089] Further replicon assays were performed with compound 8a of which the protocols and results are disclosed below.

[0090] Assay 1

[0091] The anti-HCV activity of compound 8a was tested in cell culture with replicon cells generated using reagents from the Bartenschlager laboratory (the HCV 1b bicistronic subgenomic luciferase reporter replicon clone ET). The protocol included a 3-day incubation of 2500 replicon cells in a 384-well format in a nine-point 1:4 dilution series of the compound. Dose response curves were generated based on the firefly luciferase read-out. In a variation of this assay, a 3 day incubation of 3000 cells in a 96-well format in a nine-point dilution series was followed by qRT-PCR Taqman detection of HCV genome, and normalized to the cellular transcript, RPL13 (of the ribosomal subunit RPL13 gene) as a control for compound inhibition of cellular transcription.

[0092] Assay 2

[0093] The anti-HCV activity of compound 8a was tested in cell culture with replicon cells generated using reagents from the Bartenschlager laboratory (the HCV 1b bicistronic subgenomic luciferase reporter replicon clone ET or Huh-Luc-Neo). The protocol included a 3-day incubation of 210.sup.4 replicon cells in a 96-well format in a six-point 1:5 dilution series of the compound. Dose response curves were generated based on the luciferase read-out.

[0094] Assay 3

[0095] The anti-HCV activity of compound 8a was tested in cell culture with replicon cells generated using reagents from the Bartenschlager laboratory (the HCV 1b bicistronic subgenomic luciferase reporter replicon clone ET or Huh-Luc-Neo). The protocol included either a 3-day incubation of 810.sup.3 cells or 210.sup.4 cells in a 96-well format in an eight-point 1:5 dilution series of the compound. Dose response curves were generated based on the luciferase read-out.

[0096] Results

[0097] Table 2 shows the average replicon results (EC.sub.50, replicon) obtained for compound 8a following assays as given above.

TABLE-US-00002 TABLE 2 Assay Average EC.sub.50 value (8a): 1 57 M (n = 8) 2 17.5 M (n = 4) 3 >100 M (n = 1)

[0098] Primary Human Hepatocyte In Vitro Assay

[0099] The anti-HCV activity of compound 8a was determined in an in vitro primary human hepatocyte assay. Protocols and results are disclosed below.

[0100] Protocol

[0101] Hepatocyte Isolation and Culture

[0102] Primary human hepatocytes (PHH) were prepared from patients undergoing partial hepatectomy for metastases or benign tumors. Fresh human hepatocytes were isolated from encapsulated liver fragments using a modification of the two-step collagenase digestion method. Briefly, encapsulated liver tissue was placed in a custom-made perfusion apparatus and hepatic vessels were cannulated with tubing attached multichannel manifold. The liver fragment was initially perfused for 20 min with a prewarmed (37 C.) calcium-free buffer supplemented with ethylene glycol tetraacetic acid (EGTA) followed by perfusion with a prewarmed (37 C.) buffer containing calcium (CaCl.sub.2), H.sub.2O.sub.2) and collagenase 0.05% for 10 min. Then, liver fragment was gently shaken to free liver cells in Hepatocyte Wash Medium. Cellular suspension was filtered through a gauze-lined funnel. Cells were centrifuged at low speed centrifugation. The supernatant, containing damaged or dead hepatocytes, non parenchymal cells and debris was removed and pelleted hepatocytes were re-suspended in Hepatocyte Wash Medium. Viability and cell concentration were determined by trypan blue exclusion test.

[0103] Cells were resuspended in complete hepatocyte medium consisting of William's medium (Invitrogen) supplemented with 100 IU/L insulin (Novo Nordisk, France), and 10% heat inactivated fetal calf serum (Biowest, France), and seeded at a density 1.8106 viable cells onto 6 well plates that had been precoated with a type I collagen from calf skin (Sigma-Aldrich, France) The medium was replaced 16-20 hours later with fresh complete hepatocyte medium supplemented with hydrocortisone hemisuccinate (SERB, Paris, France), and cells were left in this medium until HCV inoculation. The cultures were maintained at 37 C. in a humidified 5% CO.sub.2 atmosphere.

[0104] The PHHs were inoculated 3 days after seeding. JFH1-HCVcc stocks were used to inoculate PHHs for 12 hours, at a multiplicity of infection (MOI) of 0.1 ffu per cell. After a 12-hours incubation at 37 C., the inoculum was removed, and monolayers were washed 3 times with phosphate-buffered saline and incubated in complete hepatocyte medium containing 0.1% dimethylsufoxide as carrier control, 100 IU/ml of IFNalpha as negative control or else increasing concentrations of compound 8a. The cultures then were maintained during 3 days.

[0105] Quantitation of HCV RNA

[0106] Total RNA was prepared from cultured cells or from filtered culture supernatants using the RNeasy or Qiamp viral RNA minikit respectively (Qiagen SA, Courtaboeuf, France) according to the manufacturer's recommendations. HCV RNA was quantified in cells and culture supernatants using a strand-specific reverse real-time PCR technique described previously (Carrire M and al 2007):

[0107] Reverse transcription was performed using primers described previously located in the 50 NCR region of HCV genome, tag-RC1 (5-GGCCGTCATGGTGGCGAATAAGTCTAGCCATGGCGTTAGTA-3) and RC21 (5-CTCCCGGGGCACTCGCAAGC-3) for the negative and positive strands, respectively. After a denaturation step performed at 70 C. for 8 min, the RNA template was incubated at 4 C. for 5 min in the presence of 200 ng of tag-RC1 primer and 1.25 mM of each deoxynucleoside triphosphate (dNTP) (Promega, Charbonnieres, France) in a total volume of 12 l.

[0108] Reverse transcription was carried out for 60 min at 60 C. in the presence of 20 U RNaseOut (Invitrogen, Cergy Pontoise, France) and 7.5 U Thermoscript reverse transcriptase (Invitrogen), in the buffer recommended by the manufacturer. An additional treatment was applied by adding 1 l (2U) RNaseH (Invitrogen) for 20 min at 37 C.

[0109] The first round of nested PCR was performed with 2 l of the cDNA obtained in a total volume of 50 l, containing 3 U Taq polymerase (Promega), 0.5 mM dNTP, and 0.5 M RC1 (5-GTCTAGCCATGGCGTTAGTA-3) and RC21 primers for positive-strand amplification, or Tag (5-GGCCGTCATGGTGGCGAATAA-3) and RC21 primers for negative strand amplification. The PCR protocol consisted of 18 cycles of denaturation (94 C. for 1 min), annealing (55 C. for 45 sec), and extension (72 C. for 2 min). The cDNA obtained was purified using the kit from Qiagen, according to the manufacturer's instructions.

[0110] The purified product was then subjected to real-time PCR. The reaction was carried out using the LightCycler 480 SYBR Green I Master (2 con) Kit (Roche, Grenoble, France), with LC480 instruments and technology (Roche Diagnostics). PCR amplifications were performed in a total volume of 10 l, containing 5 l of Sybrgreen I Master Mix (2), and 25 ng of the 197R (5-CTTTCGCGACCCAACACTAC-3) and 104 (5-AGAGCCATAGTGGTCTGCGG-3) primers. The PCR protocol consisted of one step of initial denaturation for 10 min at 94 C., followed by 40 cycles of denaturation (95 C. for 15 sec), annealing (57 C. for 5 sec), and extension (72 C. for 8 sec).

[0111] The quantitation of 28Sr RNA by specific RT-PCR was used as an internal standard to express the results of HCV positive or negative strands per g of total hepatocyte RNA.

[0112] Specific primers for 28 S rRNA were designed using the Oligo6 software 5-TTGAAAATCCGGGGGAGAG-3(nt2717-2735) and 50-ACATTGTTCCAACATGCCAG-30 (nt 2816-2797). Reverse transcription was performed using AMV reverse transcriptase (Promega), and the PCR protocol consisted of one step of initial denaturation for 8 min at 95 C., followed by 40 cycles of denaturation (95 C. for 15 sec), annealing (54 C. for 5 sec), and extension (72 C. for 5 sec).

[0113] Results

[0114] Table 3 shows the anti-HCV activity of compound 8a as determined in the in vitro primary human hepatocyte assay described above. The numbers are expressed as 10.sup.6 HCV RNA copies/g of total RNA. Results of two independent experiments (Exp 1 and Exp 2) are given. The data per experiment is the average of two measurements.

[0115] Table 3: Effect of compound 8a on positive strand HCV-RNA levels in primary human hepatocytes (expressed as 10.sup.6 HCV RNA copies/g of total RNA).

TABLE-US-00003 TABLE 3 Exp. 1 Exp. 2 No HCV 0 0 HCV control 3.56 5.53 IFN (100 IU/mL) 1.48 1.59 8a (0.195 M) 2.18 1.12 8a (0.78 M) 2.25 1.3 8a (3.12 M) 1.09 0.94 8a (12.5 M) 2.17 1.3 8a(50 M) 0.94 1.33

[0116] In Vivo Efficacy Assay

[0117] The in vivo efficacy of compound 8a and CAS-1375074-52-4 was determined in a humanized hepatocyte mouse model (PBX-mouse) as previously described in Inoue et. al (Hepatology. 2007 April; 45(4):921-8) and Tenato et. al. (Am J Pathol 2004; 165-901-912) with the following specification: Test animals: HCV G1a-infected PXB-mice, male or female, >70% replacement index of human hepatocytes. Dosing was performed p.o for 7 days at doses indicated below wherein QD represents a single dose per day, BID represents two doses per day.

[0118] Efficacy of compound 8a was compared to CAS-1375074-52-4. Results are indicated in FIG. 1. The FIGURE shows the log drop HCV viral RNA after dosing for a period of 7 days.

[0119] FIG. 1 clearly shows that a dosing of 100 mg/kg QD for CAS 1375074-52-4 (indicated as *, n=4) does not result in a significant log drop in HCV viral RNA. This in strong contrast to each of the indicated dose regimens for compound 8a, were a clear log drop is observed for 100 mg/kg QD (indicated as .diamond-solid., n=3), 200 mg/kg QD (indicated as .circle-solid., n=4), 50 mg/kg BID (indicated as .square-solid., n=4). The most pronounced log drop effect in viral RNA is observed after a 7 day dosing of compound 8a at 100 mg/kg BID (indicated as .box-tangle-solidup., n=4).