Crystal form of pyrrolidinyl urea derivative and application thereof

Abstract

Disclosed in the present invention are a crystal form of a TrkA inhibitor and a preparation method thereof, and an application thereof in preparation of drugs for treating diseases associated with pain, cancer, inflammation, neurodegenerative diseases, and certain infectious diseases. ##STR00001##

Claims

1. A crystal form E of a compound of formula (II), wherein the X-ray powder diffraction pattern thereof comprises characteristic diffraction peaks at the following angles of 2θ: 9.56°±0.20°, 12.08°±0.20°, 19.29°±0.20°, wherein, n is 0 or 2, ##STR00012##

2. A crystal form E of a compound of formula (I), wherein the X-ray powder diffraction pattern thereof comprises characteristic diffraction peaks at the following angles of 2θ: 9.56°±0.20°, 12.08°±0.20° and 19.29°±0.20°, ##STR00013##

3. The crystal form E as claimed in claim 1, wherein the X-ray powder diffraction pattern thereof comprises characteristic diffraction peaks at the following angles of 2θ: 9.56±0.20°, 10.75±0.20°, 12.08±0.20°, 14.78±0.20°, 15.60±0.20°, 19.29±0.20°, 20.55±0.20° and 22.82±0.20°.

4. The crystal form E as claimed in claim 3, wherein the X-ray powder diffraction pattern thereof comprises characteristic diffraction peaks at the following angles of 2θ: 4.91°, 9.56°, 10.75°, 12.08°, 13.70°, 14.78°, 15.60°, 17.62°, 19.29°, 19.78°, 20.55°, 21.58°, 22.82°, 23.85°, 24.29°, 24.74°, 25.86°, 26.59°, 27.70°, 28.56°, 28.94°, 30.67°, 31.50° and 37.80°.

5. The crystal form E as claimed in claim 4, wherein the XRPD pattern thereof is shown in FIG. 13.

6. The crystal form E as claimed in claim 1, wherein the differential scanning calorimetry curve has an endothermic peak at 94.0±3.0° C., 154.0±3.0° C. and 171.7±3.0° C. respectively and an exothermic peak at 123.8±3.0° C.

7. The crystal form E as claimed in claim 6, wherein the DSC pattern thereof is shown in FIG. 14.

8. The crystal form E as claimed in claim 1, wherein the thermogravimetric analysis curve thereof has a weight loss of 5.84% occurred at 130.0±3° C.

9. The crystal form E as claimed in claim 8, wherein a TGA pattern thereof is shown in FIG. 15.

10. A preparation method for a crystal form E of a compound of formula (I), comprising adding any form of the compound of formula (I) into an alcohol solvent, a mixed solvent of the alcohol solvent and water or a mixed solvent of acetonitrile and water, stirring at a certain temperature for a certain time, then centrifuging, and drying a residue to obtain the crystal form E of the compound of formula (I): ##STR00014##

11. The preparation method as claimed in claim 10, wherein the volume ratio of the alcohol solvent to water or acetonitrile to water is selected from 1:1 to 4.

12. The preparation method as claimed in claim 10, wherein the alcohol solvent is selected from methanol and ethanol.

13. The preparation method as claimed in claim 10, wherein the temperature for the stirring is selected from 20° C. to 60° C.

14. The preparation method as claimed in claim 10, wherein the time for the stirring is selected from 48 hours to 96 hours.

15. The preparation method as claimed in claim 10, wherein the weight-to-volume (mg/mL) ratio of the compound of formula (I) to the solvent is selected from 10 to 100:1.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is the XRPD pattern of the crystal form A of the compound of formula (I) measured by Cu-Kα radiation.

(2) FIG. 2 is the DSC pattern of the crystal form A of the compound of formula (I).

(3) FIG. 3 is the TGA pattern of the crystal form A of the compound of formula (I).

(4) FIG. 4 is the XRPD pattern of the crystal form B of the compound of formula (I) measured by Cu-Kα radiation.

(5) FIG. 5 is the DSC pattern of the crystal form B of the compound of formula (I).

(6) FIG. 6 is the TGA pattern of the crystal form B of the compound of formula (I).

(7) FIG. 7 is the XRPD pattern of the crystal form C of the compound of formula (I) measured by Cu-Kα radiation.

(8) FIG. 8 is the DSC pattern of the crystal form C of the compound of formula (I).

(9) FIG. 9 is the TGA pattern of the crystal form C of the compound of formula (I).

(10) FIG. 10 is the XRPD pattern of the crystal form D of the compound of formula (I) measured by Cu-Kα radiation.

(11) FIG. 11 is the DSC pattern of the crystal form D of the compound of formula (I).

(12) FIG. 12 is the TGA pattern of the crystal form D of the compound of formula (I).

(13) FIG. 13 is the XRPD pattern of the crystal form E of the compound of formula (I) measured by Cu-Kα radiation.

(14) FIG. 14 is the DSC pattern of the crystal form E of the compound of formula (I).

(15) FIG. 15 is the TGA pattern of the crystal form E of the compound of formula (I).

(16) FIG. 16 is the XRPD pattern of the crystal form F of the compound of formula (I) measured by Cu-Kα radiation.

(17) FIG. 17 is the DSC pattern of the crystal form F of the compound of formula (I).

(18) FIG. 18 is the TGA pattern of the crystal form F of the compound of formula (I).

(19) FIG. 19 is the DVS pattern of the crystal form E of the compound of formula (I).

(20) FIG. 20 is the XRPD pattern of the crystal of the compound of formula (II) measured by Cu-Kα radiation.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

(21) In order to better understand the contents of the present disclosure, the following embodiments further illustrate the present disclosure, but the present disclosure is not limited thereto.

Embodiment 1: Preparation of the Compound of Formula (I)

(22) ##STR00008## ##STR00009## ##STR00010## ##STR00011##

(23) Step 1: Synthesis of Compound 2

(24) Compound 1 (15 g, 60.00 mmol) and trimethylsilyl ethylene (12.03 g, 120.01 mmol) were dissolved in acetonitrile (150 mL), and an activated copper powder (190.65 mg, 3.00 mmol) was added thereto. The reaction solution was heated to 65° C. and continued to stir for 15 hours. The mixture was cooled, and an organic solvent was removed under reduced pressure, and the obtained crude product was purified by silica gel column chromatography (eluent: 0-5% ethyl acetate/petroleum ether) to obtain compound 2. .sup.1H NMR (400 MHz, CDCl.sub.3): 4.18 (q, J=7.2 Hz, 2H), 2.94-2.90 (m, 1H), 2.52-2.37 (m, 2H), 1.20 (t, J=7.2 Hz, 3H), 0.00 (s, 9H).

(25) Step 2: Synthesis of Compound 3

(26) Compound 2 (5 g, 14.28 mmol) was dissolved in anhydrous tetrahydrofuran (100 mL) at −30° C., and diisobutyl aluminum hydride (1 M, 28.55 mL) was slowly added dropwise. The reaction solution was slowly heated to 20° C. and continued to stir for 2 hours. 60 mL of 0.5 N aqueous hydrochloric acid solution was added to the reaction solution, and the mixture was extracted with ethyl acetate (100 mL*2). The combined organic phase was washed with 200 mL of brine, dried with anhydrous sodium sulfate, and filtered, and the organic solvent was removed under reduced pressure to obtain a crude product of compound 3, which was directly used for the next reaction without further purification.

(27) Step 3: Synthesis of Compound 5

(28) Under the condition of an ice-water bath, compound 4 (8.7 g, 45.02 mmol) was dissolved in dichloromethane (60 mL), then triethylamine (13.67 g, 135.06 mmol) was added thereto, and methanesulfonyl chloride (11.35 g, 99.05 mmol) was slowly added dropwise. The reaction solution was slowly heated to 25° C. and continued to stir for 3 hours. 80 mL of water was added to the reaction solution, and the mixture was extracted with dichloromethane (80 mL*2). The combined organic phase was washed with 150 mL of brine, dried with anhydrous sodium sulfate, filtered, and the organic solvent was removed under reduced pressure. The obtained crude product was purified by silica gel column chromatography (eluent: 20-50% ethyl acetate/petroleum ether) to obtain compound 5. .sup.1H NMR (400 MHz, CDCl.sub.3): 7.38-7.21 (m, 5H), 5.12 (t, J=4.8 Hz, 2H), 3.70-3.55 (m, 2H), 3.14-3.08 (m, 2H), 3.07 (s, 6H), 2.75 (dd, J=4.0, 10.8 Hz, 2H).

(29) Step 4: Synthesis of Compound 6

(30) Compound 5 (15 g, 42.93 mmol) was dissolved in N,N-dimethylformamide (100 mL), and sodium azide (8.37 g, 128.78 mmol) was added thereto. The reaction solution was heated to 100° C. and continued to stir for 16 hours. The mixture was cooled, and 200 mL of water was added to the reaction solution and the mixture was extracted with ethyl acetate (200 mL*3). The combined organic phase was washed with water (300 mL*2) and brine (300 mL) in turn, dried with anhydrous sodium sulfate, filtered, and the organic solvent was removed under reduced pressure. The obtained crude product was purified by silica gel column chromatography (eluent: 0-2% ethyl acetate/petroleum ether) to obtain compound 6. .sup.1H NMR (400 MHz, CDCl.sub.3): 7.43-7.28 (m, 5H), 3.90 (t, J=4.4 Hz, 2H), 3.75-3.61 (m, 2H), 3.02 (dd, J=6.4, 10.0 Hz, 2H), 2.70-2.58 (m, 2H),

(31) Step 5: Synthesis of Compound 7

(32) Compound 6 (7 g, 28.77 mmol) was dissolved in tetrahydrofuran (60 mL), then water (1.04 g, 57.55 mmol) was added thereto, and triphenylphosphine (6.79 g, 25.90 mmol) was slowly added in batches. The reaction solution was stirred at 25° C. until no gas was released, heated to 80° C. and continued to stir for 1 hour. The mixture was cooled, and the organic solvent was removed under reduced pressure. 80 mL of 4 N aqueous hydrochloric acid solution was added to the obtained crude product, and the mixture was extracted with 80 mL of dichloromethane. The pH of the aqueous phase was adjusted to about 10 with ammonia water, and extracted with dichloromethane (80 mL*2). The combined organic phase was washed with 100 mL of brine, dried with anhydrous sodium sulfate, and filtered, and the organic solvent was removed under reduced pressure to obtain a crude product of compound 7, which was directly used for the next reaction without further purification. .sup.1H NMR (400 MHz, CDCl.sub.3): 7.35-7.24 (m, 5H), 3.64 (q, J=13.2 Hz, 2H), 3.56 (td, J=3.6, 6.8 Hz, 1H), 3.48-3.40 (m, 1H), 3.07-2.90 (m, 2H), 2.64 (dd, J=4.4, 10.4 Hz, 1H), 2.31 (dd, J=5.2, 9.6 Hz, 1H).

(33) Step 6: Synthesis of Compound 8

(34) Compound 7 (6.4 g, 29.46 mmol) was dissolved in dichloromethane (60 mL), and triethylamine (5.96 g, 58.91 mmol) and di-tert-butyl dicarbonate (7.71 g, 35.35 mmol) were added thereto. The reaction solution was continued to stir at 25° C. for 15 hours. The organic solvent was removed under reduced pressure, and the obtained crude product was purified by silica gel column chromatography (eluent: 0-10% ethyl acetate/petroleum ether) to obtain compound 8. .sup.1H NMR (400 MHz, CDCl.sub.3): 7.40-7.25 (m, 5H), 4.87 (s, 1H), 4.07 (s, 1H), 3.81 (s, 1H), 3.70-3.56 (m, 2H), 3.07 (dd, J=6.8, 10.4 Hz, 1H), 2.93-2.77 (m, 1H), 2.56-2.32 (m, 2H), 1.47 (s, 9H).

(35) Step 7: Synthesis of Compound 9

(36) Compound 8 (8.8 g, 27.73 mmol) was dissolved in methanol (100 mL), and palladium carbon (0.5 g, 27.73 mmol, 10% purity) was added thereto. The reaction solution was continued to stir at 20° C. for 3 hours under a hydrogen pressure of 15 psi. The reaction solution was filtered through diatomite, and the organic solvent was removed under reduced pressure to obtain a crude product of compound 9, which was be directly used for the next reaction without further purification. .sup.1H NMR (400 MHz, CDCl.sub.3): 7.33-7.25 (m, 5H), 5.04 (d, J=6.4 Hz, 1H), 3.70 (s, 1H), 3.63-3.54 (m, 2H), 3.34-3.23 (m, 1H), 3.07 (t, J=8.4 Hz, 1H), 2.82 (dd, J=7.2, 9.6 Hz, 1H), 2.51-2.43 (m, 1H), 2.21-2.09 (m, 1H), 1.44 (s, 9H).

(37) Step 8: Synthesis of Compound 10

(38) Compound 9 (2.35 g, 8.06 mmol) was dissolved in acetonitrile (50 mL), and compound 3 (1.98 g, 6.45 mmol) was added thereto. The reaction solution was heated to 50° C. and continued to stir for 15 hours. The mixture was cooled, and the organic solvent was removed under reduced pressure. The obtained crude product was purified by silica gel column chromatography (eluent: 0-30% ethyl acetate/petroleum ether) to obtain compound 10. .sup.1H NMR (400 MHz, CDCl.sub.3): 7.40-7.27 (m, 5H), 6.61 (s, 1H), 6.53 (s, 1H), 5.87 (dd, J=2.0, 2.8 Hz, 1H), 4.82 (s, 1H), 4.29-4.17 (m, 1H), 4.15-4.05 (m, 1H), 3.73-3.58 (m, 2H), 3.16-3.03 (m, 2H), 2.85-2.76 (m, 1H), 2.58-2.43 (m, 1H), 1.43 (s, 9H).

(39) Step 9: Synthesis of Compound 11

(40) Compound 10 (840 mg, 2.34 mmol) was dissolved in toluene (30 mL), and diisopropylethylamine (422.86 mg, 3.27 mmol) was added thereto. Under the condition of an ice-water bath, 1-chloroethyl chloroformate (434.35 mg, 3.04 mmol) was slowly added dropwise. The reaction solution was heated to 90° C. and continued to stir for 1 hour. The reaction solution was cooled, and the organic solvent was removed under reduced pressure. Methanol (30 mL) was added thereto, and the mixture was stirred at 20° C. for 17 hours. The organic solvent was removed under reduced pressure to obtain a crude product of compound 11, which was directly used in the next reaction without further purification. MS m/z: 270.1 [M+1].sup.+.

(41) Step 10: Synthesis of Compound 12

(42) Compound 11 (630 mg, 2.34 mmol) was dissolved in N,N-dimethylformamide (10 mL), and diisopropylethylamine (906.98 mg, 7.02 mmol) and 2-bromoethyl methyl ether (536.92 mg, 3.51 mmol) were added thereto. The reaction solution was continued to stir at 20° C. for 64 hours. The reaction solution was diluted with 100 mL of ethyl acetate, washed with 60 mL of water and 60 mL of brine in turn, dried with anhydrous sodium sulfate, and filtered. The organic solvent was removed under reduced pressure. The obtained crude product was purified by silica gel column chromatography (eluent: 25%-60% ethyl acetate/petroleum ether) to obtain compound 12. .sup.1H NMR (400 MHz, CDCl.sub.3): 6.60 (s, 1H), 6.54 (s, 1H), 5.88 (dd, J=2.0, 2.8 Hz, 1H), 4.91 (s, 1H), 4.24 (s, 1H), 4.12-4.05 (m, 1H), 3.51 (t, J=5.6 Hz, 2H), 3.37 (s, 3H), 3.19 (s, 1H), 3.08 (d, J=8.0 Hz, 1H), 2.84-2.64 (m, 3H), 1.43 (s, 9H).

(43) Step 11: Synthesis of Compound 13

(44) Compound 12 (100 mg, 305.44 μmol) was dissolved in dichloromethane (2 mL), and trifluoroacetic acid (2 mL) was added thereto. The reaction solution was continued to stir at 20° C. for 0.5 hours. The organic solvent was removed under reduced pressure to obtain a crude product of compound 13, which was directly used in the next reaction without further purification. MS m/z: 228.1 [M+1].sup.+.

(45) Step 12: Synthesis of Compound 15

(46) Compound 14 (4.0 g, 14.04 mmol) was dissolved in anhydrous tetrahydrofuran (40 mL), cooled to −78° C. Oxetanone (1.2 g, 16.85 mmol) was added thereto, and n-butyl lithium (2.5 M, 8.4 mL) solution was slowly added thereto dropwise. The reaction solution was continued to stir at this temperature for 20 minutes. Saturated aqueous ammonium chloride solution (20 mL) was slowly added to the reaction solution, and the mixture was extracted with ethyl acetate (50 mL*3). The combined organic phases were washed with saturated sodium chloride solution (50 mL), dried with anhydrous sodium sulfate, filtered, and the organic solvent was removed under reduced pressure. The obtained crude product was purified by silica gel column chromatography (eluent: 0-20% ethyl acetate/petroleum ether) to obtain compound 15. .sup.1H NMR: (400 MHz, CDCl.sub.3): 8.89 (s, 2H), 5.06-4.95 (m, 4H).

(47) Step 13: Synthesis of Compound 16

(48) Compound 15 (1.8 g, 7.75 mmol) was dissolved in dichloromethane (13 mL) under an ice water bath, and a dichloromethane (4 mL) solution of diethylaminosulfur trifluoride (2.5 g, 15.50 mmol) was added thereto. The reaction solution was continued to stir at this temperature for 20 minutes. The reaction solution was added with water (20 mL), and extracted with ethyl acetate (50 mL*3). The combined organic phases were washed with saturated sodium chloride solution (50 mL), dried with anhydrous sodium sulfate, filtered, and the organic solvent was removed under reduced pressure. The obtained crude product was purified by silica gel column chromatography (eluent: 0-10% ethyl acetate/petroleum ether) to obtain compound 16. .sup.1H NMR (400 MHz, CDCl.sub.3): 8.91 (s, 2H), 5.20-5.05 (m, 4H).

(49) Step 14: Synthesis of Compound 17

(50) Compound 16 (300 mg, 1.29 mmol) was dissolved in 1,4-dioxane (8.0 mL), and then bis(pinacolato)diboron (392 mg, 1.54 mmol) and potassium acetate (379 mg, 3.86 mmol) were added thereto in turn. The reaction system was replaced with nitrogen for three times, and then 1,1-bis(diphenylphosphino)ferrocene palladium dichloride (94 mg, 128.73 μmol) was added thereto. The reaction solution was heated to 100° C. and continued to stir for 11 hours. The mixture was cooled, and the organic solvent was removed under reduced pressure. The obtained crude product was purified by silica gel column chromatography (eluent: 0-50% ethyl acetate/petroleum ether) to obtain compound 17. .sup.1H NMR (400 MHz, CDCl.sub.3): 9.11 (s, 2H), 5.24-5.05 (m, 4H), 1.37 (s, 12H).

(51) Step 15: Synthesis of Compound 19

(52) Compound 18 (20.00 g, 184.95 mmol) and ethyl 2-cyanopropionate (23.51 g, 184.95 mmol) were dissolved in 1,4-dioxane (40 mL), and the reaction solution was heated to 110° C. and continued to stir for 72 hours. The mixture was cooled, and the reaction solution was concentrated to about 20 mL to precipitate a solid, filtered. The filter cake was washed with ethyl acetate (30 mL), and the filter cake was collected to obtain compound 19. .sup.1H NMR (400 MHz, CD.sub.3OD): 7.53-7.46 (m, 2H), 7.42-7.35 (m, 3H), 1.77 (s, 3H).

(53) Step 16: Synthesis of Compound 20

(54) Compound 19 (10.00 g, 52.85 mmol) was dissolved in N,N-dimethylformamide (150 mL), and N,N-diisopropylethylamine (20.49 g, 158.55 mmol) and N-phenylbis(trifluoromethanesulfonyl)imide (19.82 g, 55.49 mmol) were added thereto in turn. The reaction solution was continued to stir at 25° C. for 16 hours. The reaction solution was poured into 500 mL of water, then extracted with ethyl acetate (150 mL*3), and the organic phases were combined. The organic phase was washed with brine (300 mL), dried with anhydrous sodium sulfate, filtered, and the filtrate was concentrated to dryness to obtain a crude product of compound 20. .sup.1H NMR (400 MHz, CDCl.sub.3): 7.54-7.44 (m, 4H), 7.40-7.34 (m, 1H), 3.76 (s, 2H), 1.95 (s, 3H).

(55) Step 17: Synthesis of Compound 21

(56) Compound 20 (320 mg, 1.14 mmol) was dissolved in a mixed solution of dioxane (2.5 mL) and water (0.5 mL), and compound 17 (293 mg, 912.00 μmol) and 1,1-bis(diphenylphosphino)ferrocene palladium dichloride (83 mg, 114.00 μmol) were added thereto, and finally sodium carbonate (242 mg, 2.28 mmol) was added thereto. The reaction system was replaced with nitrogen three times. The reaction solution was heated to 100° C. and stirred for 14 hours. The reaction solution was dried with anhydrous sodium sulfate, filtered and concentrated. The crude product was purified by column chromatography (eluent: 0-25% ethyl acetate/petroleum ether) to obtain compound 21. .sup.1H NMR (400 MHz, CDCl.sub.3): 9.11 (s, 2H), 7.64-7.27 (m, 5H), 5.32-4.88 (m, 4H), 3.67 (s, 2H), 2.10 (s, 3H).

(57) Step 18: Synthesis of the Compound of Formula (I)

(58) Compound 21 (60 mg, 184.42 μmol) was dissolved in dichloromethane (5 mL), and triphosgene (43.78 mg, 147.54 μmol) and N,N-diisopropylethylamine (71.50 mg, 553.27 μmol, 96.37 μL) were added thereto. The reaction solution was stirred at 20° C. for 20 minutes. Compound 13 (139.72 mg, 184.42 μmol) and N,N-diisopropylethylamine (71.50 mg, 553.27 μmol, 96.37 μL) were added thereto, and the reaction solution was continued to stir at 20° C. for 15 hours. The organic solvent was removed under reduced pressure, and the obtained crude product was purified by high performance liquid chromatography (chromatographic column: Xtimate C18 150*25 mm*5 μm; mobile phase: [water (10 mM ammonium bicarbonate)-acetonitrile]; acetonitrile %: 37%-58%, 10.5 min) to obtain the compound of formula (I). .sup.1H NMR (400 MHz, CD.sub.3OD): 9.28 (s, 2H), 7.67-7.40 (m, 5H), 6.66-6.49 (m, 2H), 5.84 (s, 1H), 5.33-5.20 (m, 2H), 5.14-5.00 (m, 2H), 4.34-4.16 (m, 2H), 3.53 (t, J=5.2 Hz, 2H), 3.37 (s, 3H), 3.17-3.05 (m, 2H), 2.87-2.78 (m, 1H), 2.79-2.63 (m, 2H), 2.58-2.47 (m, 1H), 2.23 (s, 3H); MS m/z=579.4 [M+1].sup.+.

Embodiment 2: Preparation of the Crystal Form A of the Compound of Formula (I)

(59) 50 mg of the compound of formula (I) was added into a 4.0 mL glass vial, and 1 mL of water was added thereto to make the mixture into a suspension. After a magneton was added, a sample of the above suspension was placed on a magnetic heating stirrer (50° C.) for testing, stirred at 50° C. for 48 hours, and then centrifuged. The residue was placed in a vacuum drying oven (60° C.) to dry overnight to obtain the crystal form A of the compound of formula (I).

(60) 50 mg of the compound of formula (I) was added into a 4.0 mL glass vial, and 1 mL of a mixed solvent of acetone and water (1:4) was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (50° C.) for testing, stirred at 20° C. for 96 hours, and then centrifuged. The residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form A of the compound of formula (I).

Embodiment 3: Preparation of the Crystal Form B of the Compound of Formula (I)

(61) 50 mg of the compound of formula (I) was weighted and added into a 4.0 mL glass vial, and 1 mL of ethanol was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (50° C.) for testing. After stirring at 50° C. for 48 hours, the solution was clarified. After the solvent was volatilized, the residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form B of the compound of formula (I).

(62) 50 mg of the compound of formula (I) was weighted and added into a 4.0 mL glass vial, and 1 mL of acetonitrile was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (20° C.) for testing. After stirring at 20° C. overnight, the solution was clarified. After the solvent was volatilized, the residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form B of the compound of formula (I).

Embodiment 4: Preparation of the Crystal Form C of the Compound of Formula (I)

(63) 50 mg of the compound of formula (I) was weighted and added into a 4.0 mL glass vial, and 1 mL of ethyl acetate was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (50° C.) for testing. After stirring at 50° C. for 48 hours, the solution was clarified. Half of the solution was taken and added with 20 mg of the compound of formula (I), stirred at 20° C. for 48 hours, and then centrifuged. The residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form C of the compound of formula (I).

Embodiment 5: Preparation of the Crystal Form D of the Compound of Formula (I)

(64) 50 mg of the compound of formula (I) was weighted and added into a 4.0 mL glass vial, and 1 mL of ethyl acetate was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (50° C.) for testing. After stirring at 50° C. for 48 hours, the solution was clarified. After the solvent was volatilized, the residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form D of the compound of formula (I).

Embodiment 6: Preparation of the Crystal Form E of the Compound of Formula (I)

(65) 50 mg of the compound of formula (I) was added into a 4.0 mL glass vial, and 1 mL of a mixed solvent of methanol and water (1:1) was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (50° C.) for testing, stirred at 50° C. for 48 hours, and then centrifuged. The residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form E of the compound of formula (I).

(66) 50 mg of the compound of formula (I) was added into a 4.0 mL glass vial, and 1 mL of methanol was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (20° C.) for testing, stirred at 20° C. for 96 hours, and then centrifuged. The residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form E of the compound of formula (I).

(67) 50 mg of the compound of formula (I) was added into a 4.0 mL glass vial, and 1 mL of a mixed solvent of acetonitrile and water (1:1) was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (20° C.) for testing, stirred at 20° C. for 96 hours, and then centrifuged. The residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form E of the compound of formula (I).

(68) 50 mg of the compound of formula (I) was added into a 4.0 mL glass vial, and 1 mL of ethanol was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (50° C.) for testing. After stirring at 50° C. for 48 hours, the solution was clarified. Half of the solution was taken and added with 20 mg of the compound of formula (I), stirred at 20° C. for 48 hours, and then centrifuged. The residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form E of the compound of formula (I).

(69) 100 mg of the compound of formula (I) was added into a 4.0 mL glass vial, and 1 mL of a mixed solvent of ethanol and water (1:4) was added thereto to make the mixture into a suspension. After the magneton was added, a sample of the above suspension was placed on the magnetic heating stirrer (40° C.) for testing, stirred at 40° C. for 60 hours, and then centrifuged. The residue was placed in the vacuum drying oven (60° C.) to dry overnight to obtain the crystal form E of the compound of formula (I).

Embodiment 7: Preparation of the Crystal Form F of the Compound of Formula (I)

(70) 17 g of the compound of formula (I) was added into a 1 L glass bottle, and 200 mL of a mixed solvent of ethanol and water (1:4) was added thereto to make the mixture into a suspension. A sample of the above suspension was placed on the magnetic heating stirrer (50° C.) for testing, stirred at 50° C. for 24 hours, and then filtered. The filter cake was collected, and the obtained sample was placed in the vacuum drying oven (60° C.) to dry overnight after repeating the above operation for 5 times to obtain the crystal form F of the compound of formula (I).

Embodiment 8: Solid Stability Test of the Crystal Form E of the Compound of Formula (I)

(71) According to the “Guidelines for the Stability Test of APIs and Preparations” (Chinese Pharmacopoeia 2015 Edition Part IV general rules 9001), the stability of the crystal form E of the compound of formula (I) was investigated at high temperature (60° C., opened), high humidity (room temperature/relative humidity 92.5%, opened) and strong illumination (5000 1×, closed).

(72) 10 parts of the crystal form E of the compound of formula (I) were weighed in parallel, each of which was about 15 mg, and placed at the bottom of a glass sample bottle to spread into a thin layer. The 10 parts of the crystal form E were placed under conditions of high temperature (60° C.), high humidity (92.5% humidity, room temperature), high temperature and high humidity (60° C./75% humidity, 60° C./75% humidity) and stable illumination, respectively. Samples placed under high temperature and high humidity conditions were sealed with aluminum foil paper and small holes were punched in the aluminum foil paper to ensure that the sample could fully contact with ambient air; samples placed under strong illumination conditions were sealed with threaded caps. Samples placed under high temperature (60° C.) and high humidity (92.5% humidity, room temperature) conditions were sampled and tested (XRPD and purity) on the 5th and 10th day. Samples placed under the high temperature and high humidity (60° C./75% humidity, 60° C./75% humidity) were sampled and tested (XRPD and purity) on the 30th and 60th days. Samples placed under the condition of illumination were sampled and tested when the total illuminance reached 1.2×10.sup.6 Lux.Math.hr. The test results were compared with the initial test results of 0 day, and the test results were shown in Table 7 below:

(73) TABLE-US-00010 TABLE 7 Solid stability test results of the crystal form E of the compound of formula (I) Time Purity Test condition point Crystal form (%) — 0 day Crystal form E 98.75% High temperature (60° C.,  5 days Crystal form E 98.74% opened) 10 days Crystal form E 98.74% High humidity (room  5 days Crystal form E 98.62% temperature/relative humidity 10 days Crystal form E 98.72% 92.5%, opened) Illumination (total illuminance — Crystal form E 98.83% 1.2 × 10.sup.6 Lux .Math. hr/near ultraviolet 200 w .Math. hr/m.sup.2, opened) 40° C., relative humidity 75%, 30 days Crystal form E 98.14% opened 60 days Crystal form E 98.06% 60° C., relative humidity 75%, 30 days Crystal form E 98.17% opened 60 days Crystal form E 98.22%

(74) Conclusion: The crystal form E of the compound of formula (I) has good stability under the conditions of high temperature, high humidity, strong illumination and accelerated conditions.

Embodiment 9: Study on the Hygroscopicity of the Crystal Form E of the Compound of Formula (I)

(75) Experimental Materials:

(76) Intrinsic dynamic vapor sorption instrument

(77) Experimental Method:

(78) 10 to 15 mg of the crystal form E of the compound of formula (I) was placed in a DVS sample tray for testing.

(79) Experimental Results:

(80) The DVS pattern of the crystal form E of the compound of formula (II) was shown in the FIG. 19, where ΔW=6.228%.

(81) Experimental Conclusion:

(82) The hygroscopic weight gain of the crystal form E of the compound of formula (I) at 25° C. and 80% RH was 6.228%, which was hygroscopic.

Experimental Embodiment 1: TrkA Enzyme Activity Test

(83) Experimental Materials

(84) TrkA Invitrogen-PV4114

(85) TK detection kit Cisbio-62TK0PEJ

(86) Test plate PerkinElmer-6007299

(87) Envision PerkinElmer-2104

(88) Kinase Reaction Buffer

(89) 50 mM Hepes (pH 7.5), 5 mM MgCl.sub.2 (magnesium chloride), 0.01 mM orthovanadate (sodium vanadate), 1% BSA (bovine serum albumin), 1 mM (dithiothreitol)

(90) Experimental Method:

(91) This experiment used Cisbio's homogeneous time-resolved fluorescence resonance energy transfer (HTRF® method) for activity test. In the test plate, the enzyme, biotin-labeled peptide substrate, ATP, and test compound were mixed, and incubated for reaction. After the reaction, ethylenediaminetetraacetic acid was added to terminate the reaction; at the same time, Eu-labeled antibody and streptavidin-labeled XL665 were added for reaction and detection. The data were expressed by the readings of the fluorescence signal at 665 nm and 620 nm, where a high ratio of 665 nm/620 nm indicated higher activity, and a low ratio of 665 nm/620 nm indicated inhibition of activity.

(92) Experimental Procedures

(93) 1. Dilution of compound: the test compound was 3-fold diluted to prepare a total of 11 concentrations, the concentration was from 10 μM to 0.17 nM in the final system;

(94) 2. A 10 μL reaction system with a buffer of 50 mM Hepes (pH 7.5), 5 mM MgCl.sub.2, 0.01 mM sodium vanadate, 1% BSA, and 1 mM DTT, and containing 0.5 nM TrkA kinase, 0.3 μM biotin-TK peptide (biotin-labeled tyrosine kinase substrate peptide), 90 μM ATP was incubated at 23° C. for 90 minutes. 10 μL of stop solution containing 20 mM EDTA, 1.34 nM phosphorylated substrate antibody, and 100 nM streptavidin-labeled fluorescent molecule XL-665 was then added, incubated at 23° C. for 60 minutes; the signal was read with Envision;

(95) 3. The inhibition rate of the compound was calculated from the data read by the instrument, and the IC.sub.50 value was calculated using the mode 205 in XLFIT5 of IDBS.

(96) Experimental Results

(97) The results are shown in Table 8.

(98) TABLE-US-00011 TABLE 8 IC.sub.50 value of the compound of formula (I) inhibiting TrkA enzyme Number of the compound TrkA IC.sub.50 (nM) Compound of formula (I) 0.56

(99) The results show that the compound of formula (I) has a significant inhibitory effect on TrkA enzyme.

Experimental Embodiment 2: Plasma Protein Binding Rate (PPB) Test

(100) Experimental Objective

(101) The protein binding rates of the test compound in the plasma of humans and SD rats were determined.

(102) Experimental Operation

(103) 796 μL of blank plasma from humans and SD rats (plasma purchased from BioreclamationIVT) were taken, 4 μL of working solution of the test compound (400 μM) or warfarin working solution (400 μM) was added and the final concentration of the test compound or warfarin was 2 μM. The sample was mixed thoroughly. The final concentration of organic phase DMSO was 0.5%; 50 μL of the plasma sample treated with the test compound or warfarin was pipetted into the sample receiving plate, and a corresponding volume of blank plasma or buffer was added immediately so that the final volume in each sample well was 100 μL, the volume ratio of plasma: dialysis buffer was 1:1; 400 μL of stop solution was added to these samples, and these samples were used as To samples for recovery and stability determination. To samples were stored at 2° C. to 8° C. for subsequent processing together with other dialyzed samples; 150 μL of the plasma sample treated with the test compound or warfarin was added into the dosing end of each dialysis hole, and 150 μL of blank dialysis buffer was added to the receiving end corresponding to the dialysis hole. Then the dialysis plate was sealed with gas-permeable membrane and placed in a humidified 5% CO.sub.2 incubator, and incubated at 37° C. with 100 rpm shaking for 4 hours. After the dialysis, 50 μL of the dialysis buffer sample and the dialysis plasma sample were transferred to a new sample receiving plate. A corresponding volume of blank plasma or buffer was added to the sample, so that the final volume was 100 μL in each sample well, and the volume ratio of plasma:dialysis buffer was 1:1. All samples were analyzed by LC/MS/MS after protein precipitation, and the plasma protein unbound rate, binding rate and recovery rate was calculated according to the following formula: % unbound rate=100*free compound concentration at the membrane buffer side/total compound concentration at the membrane plasma side, % protein binding rate=100−% unbound rate, % recovery rate=100*(free compound concentration at the membrane buffer side+total compound concentration at the membrane plasma side)/total compound concentration before dialysis.

(104) Experimental Results

(105) The results are shown in Table 9.

(106) TABLE-US-00012 TABLE 9 Human and rat plasma protein unbound rates of the compound of formula (I) Plasma protein unbound rate Number of the compound Human plasma SD rat plasma Compound of formula (I) 13.2% 7.1%

(107) The results show that the compound of formula (I) has a high plasma protein unbound rate.

Experimental Embodiment 3: Cytochrome P450 Isozyme Inhibitory Activity Test

(108) Experimental Objective

(109) The inhibitory activities of the test compound on different subtypes of human cytochrome P450 isozymes were determined.

(110) Experimental Operation

(111) The test compound, standard inhibitor (100× final concentration), and a mixed substrate working solution were prepared; the microsome frozen in −80° C. refrigerator was thawed. 2 μL of the test compound and standard inhibitor solution were added to the corresponding wells, and at the same time 2 μL of the corresponding solvent was added to the non-inhibitor control wells (NIC) and blank control wells (Blank) wells; then 20 μL of mixed substrate solution was added to the corresponding wells, except for the Blank wells (20 μL of PB was added to the Blank wells); a human liver microsome solution was prepared (put back into the refrigerator immediately after using and marking the date), and then 158 μL of the human liver microsome solution was added to all wells; the above sample plate was placed in a 37° C. water bath for pre-incubation, then the coenzyme factor (NADPH) solution was prepared; 10 minutes later, 20 μL of the NADPH solution was added to all wells, and the sample plate was shaken well, placed in a 37° C. water bath and incubated for 10 minutes; at the corresponding time point, 400 μL of cold acetonitrile solution (internal standard: 200 ng/mL tolbutamide and labetalol) was added to terminate the reaction; the sample plate was evenly mixed, centrifuged at 4000 rpm for 20 minutes to precipitate proteins; 200 μL of the supernatant was added to 100 μL of water, shook well, and tested by LC/MS/MS.

(112) Experimental Results

(113) The results are shown in Table 10.

(114) TABLE-US-00013 TABLE 10 IC.sub.50 value of the compound of formula (I) for P450 isozyme inhibition Cytochrome P450 isozyme IC.sub.50 (nM) Number of the compound CYP1A2 CYP2C9 CYP2C19 CYP2D6 CYP3A4 Compound of formula (I) >50 39.1 >50 40.6 >50

(115) The results show that the compound of formula (I) has a low risk of drug-drug interaction.

Experimental Embodiment 4: Metabolic Stability (MMS) Study in Liver Microsomes

(116) Experimental Objective

(117) The metabolic stability of the test compound in human and rat liver microsomes was tested.

(118) Experimental Materials

(119) Test compound (10 mM), Testosterone (reference compound, 10 mM), Diclofenac (reference compound, 10 mM) and Propafenone (reference compound, 10 mM).

(120) Buffer System

(121) 1. 100 mM potassium phosphate buffer (pH 7.4).

(122) 2. 10 mM MgCl.sub.2.

(123) Dilution of Compound

(124) 1. Intermediate solution: 45 μL of DMSO (with 450 μL of 1:1 methanol/water) was used to dilute 5 μL of the sample for test compound or reference compound.

(125) 2. Working solution: 450 μL of 100 mM potassium phosphate buffer was used to dilute the intermediate solution.

(126) NADPH Regeneration System

(127) 1. β-Phosphoramidate adenine dinucleotide, from Sigma, Cat. No. N0505.

(128) 2. Isocitric acid, from Sigma, Cat. No. 11252.

(129) 3. Isocitrate dehydrogenase, from Sigma, Cat. No. 12002.

(130) TABLE-US-00014 Preparation of a liver microsome solution (final concentration: 0.5 mg protein/mL) Microsomes Product information Source Human liver microsomes Cat No. 452117 BD Biosciences Lot No. 38291 Rat liver microsomes Cat No. R1000 Xenotech Lot No. 1310030 Canine liver microsomes Cat No. D1000 Xenotech Lot No. 1310086

(131) Stop Solution

(132) Cold acetonitrile containing 100 ng/mL tolbutamide and 100 ng/mL labetalol was used as the internal standard.

(133) Experimental Method

(134) 10 μL of the working solution of the test compound or reference compound was added to all plates (T.sub.0, T.sub.5, T.sub.10, T.sub.20, T.sub.30, T.sub.60, and NCF.sub.60).

(135) 680 μL of liver microsome solution was added to each well of 96-well plates, then 80 μL was added to each well of the plates, and the plates were placed at 37° C. for pre-incubation for approximately 10 minutes.

(136) 10 μL of 100 mM potassium phosphate buffer was added to each well of the NCF.sub.60 plate.

(137) After the pre-incubation, 90 μL NADPH regeneration system working solution was added to each well of the 96-well plates, and then 10 μL was added to each well of the plates to initiate the reaction.

(138) Incubation for an appropriate time (such as 5, 10, 20, 30, and 60 minutes).

(139) 300 μL of the stop solution (refrigerated at 4° C., containing 100 ng/mL tolbutamide and 100 ng/mL labetalol) was added into each sample well.

(140) The sample plate was shaken for about 10 minutes and centrifuged at 4000 rpm for 20 minutes at 4° C.

(141) During centrifugation, 300 μL of HPLC water was added to each well, and 100 μL of the supernatant was collected for LC-MS/MS analysis.

(142) Data Analysis

(143) The half-life T.sub.1/2 and the intrinsic clearance rate by liver microsome C.sub.lint(mic) were calculated by the following formulas:
C.sub.t=C.sub.ij.Math.e.sup.−k.sup.s.sup..Math.i when

(144) $C_t=\frac{1}{2}{C}_0,$$T_{1/2}=\frac{\mathrm{Ln}2}{k_e}=\frac{0.693}{k_e}$${\mathrm{CL}}_{\mathrm{int}\left(\mathrm{mic}\right)}=\frac{0.693}{T_{1/2}}.Math.\frac{1}{\begin{array}{c}\mathrm{Concentration}\mathrm{of}\mathrm{microsomal}\\ \mathrm{protein}\mathrm{during}\mathrm{incubation}\left(\mathrm{mg}/\mathrm{mL}\right)\end{array}}$${\mathrm{CL}}_{\mathrm{int}\left(\mathrm{liver}\right)}={\mathrm{CL}}_{\mathrm{int}\left(\mathrm{mic}\right)}.Math.\frac{\mathrm{Microsomal}\mathrm{protein}\left(\mathrm{mg}\right)}{\mathrm{Liver}\mathrm{weight}\left(g\right)}.Math.\frac{\mathrm{Liver}\mathrm{weight}\left(g\right)}{\mathrm{Body}\mathrm{weight}\left(\mathrm{kg}\right)}$
C.sub.t=C.sub.ij.Math.e.sup.−k.sup.s.sup..Math.i when

(145) $C_t=\frac{1}{2}{C}_0,$$T_{1/2}=\frac{\mathrm{Ln}2}{k_e}=\frac{0.693}{k_e}$${\mathrm{CL}}_{\mathrm{int}\left(\mathrm{mic}\right)}=\frac{0.693}{T_{1/2}}.Math.\frac{1}{\begin{array}{c}\mathrm{Concentration}\mathrm{of}\mathrm{microsomal}\\ \mathrm{protein}\mathrm{during}\mathrm{incubation}\left(\mathrm{mg}/\mathrm{mL}\right)\end{array}}$${\mathrm{CL}}_{\mathrm{int}\left(\mathrm{liver}\right)}={\mathrm{CL}}_{\mathrm{int}\left(\mathrm{mic}\right)}.Math.\frac{\mathrm{Microsomal}\mathrm{protein}\left(\mathrm{mg}\right)}{\mathrm{Liver}\mathrm{weight}\left(g\right)}.Math.\frac{\mathrm{Liver}\mathrm{weight}\left(g\right)}{\mathrm{Body}\mathrm{weight}\left(\mathrm{kg}\right)}$

(146) Each gram of liver contained 45 mg of microsomal protein, and the liver weights of mice, rats, dogs, monkeys and humans were 88 g/kg, 40 g/kg, 32 g/kg, 30 g/kg and 20 g/kg, respectively.

(147) C.sub.t is the concentration at time t, t is the incubation time, C.sub.0 is the concentration at 0, k.sub.e is the elimination rate constant, Cl.sub.int(mic) is the intrinsic clearance rate by liver microsome, and Cl.sub.int(liver) is the intrinsic clearance rate by liver.

(148) Experimental Results

(149) The results are shown in Table 11.

(150) TABLE-US-00015 TABLE 11 Clearance rate of the compound of formula (I) in human and rat liver microsomes Intrinsic clearance rate of liver microsomes (mL/min/Kg) Number of the compound Human Rat Compound of formula (I) 51.7 24.9

(151) The results show that the compound of formula (I) has good metabolic stability of liver microsomes in two species of human and rat.

Experimental Embodiment 5: In Vivo Pharmacokinetic Study of the Crystal Form E of the Compound of Formula (I) in Rats After Single Administration

(152) Experimental Objective

(153) Male SD rats were used as test animals, and the concentration of the compound in blood was determined after single administration to evaluate the pharmacokinetic behavior.

(154) Experimental Materials:

(155) Sprague Dawley rats (male, 200-300 g, 7-9 weeks, Shanghai Charles River Laboratory Animal Co., Ltd.)

(156) Experimental Operation:

(157) The pharmacokinetic characteristics of the test compound in rodents after intravenous injection and oral administration were tested by standard protocols, in the experiment, the test compound was prepared into a clear solution or homogeneous suspension, and administered to the rats by single intravenous injection and oral administration. In the intravenous injection group, the menstruum was a certain proportion of ethanol and physiological saline solution or a certain proportion of dimethyl sulfoxide in HP-β cyclodextrin solution (the pH was adjusted to 3-4), the mixture was vortexed and stirred to prepare 1 mg/mL clear solution, then filtered with microporous membrane for later use; in the oral administration group, the menstruum was a certain proportion of sodium carboxymethyl cellulose solution or a certain proportion of dimethyl sulfoxide in HP-β cyclodextrin solution (the pH was adjusted to about 4), after the test compound was mixed with the solvent, the mixture was vortexed and stirred to prepare 30 mg/mL homogeneous suspension for later use. After 2 mg/kg intravenous administration or 300 mg/kg oral administration to rats, a certain amount of whole blood samples were collected, centrifuged at 3000 g for 15 minutes, the supernatant was separated to obtain the plasma samples; then an acetonitrile solution containing internal standard with a volume 3 times that of the plasma sample was added to precipitate proteins, and then centrifuged; the supernatant was separated, and water with a volume twice that of the supernatant was added to the supernatant, which was then centrifuged again; the supernatant was collected for analysis; LC-MS/MS analysis method was used to quantitatively analyze the blood drug concentration, and Phoenix WinNonlin software (Pharsight, USA) was used to calculate the pharmacokinetic parameters, such as peak concentration, time to peak concentration, clearance rate, half-life, area under the concentration-time curve, bioavailability, etc.

(158) Experimental Results:

(159) TABLE-US-00016 TABLE 12 Oral pharmacokinetic properties of the crystal form E of the compound of formula (I) in rats Test Compound 2 mpk C.sub.0 (nM) 5385 compound of formula intravenous T.sub.1/2 (hr) 0.55 (I) injection Vd.sub.ss (L/kg) 0.81 Cl 22.4 (mL/min/kg) AUC.sub.0-inf 2611 (nM .Math. hr) Crystal form 300 mpk oral C.sub.max (nM) 48500 E of the administration T.sub.max (hr) 0.75 compound T.sub.1/2 (hr) 3.25 of formula AUC.sub.0-inf 276267 (I) (nM .Math. hr) Bioavailability 70.5%

(160) Among them, C.sub.0 is the initial concentration, T.sub.1/2 is the elimination half-life, V.sub.dss is the steady-state apparent volume of distribution, Cl is the total clearance rate, AUC.sub.0-inf is the area under the plasma drug concentration-time curve from time 0 to infinity, C.sub.max is the peak concentration, T.sub.max is the time to peak concentration.

(161) The results show that the crystal form E of the compound of formula (I) has good pharmacokinetic properties and oral bioavailability in rats.

Experimental Embodiment 6: In Vivo Pharmacokinetic Study in Mice After Single Administration

(162) Experimental Objective

(163) Male CD-1 mice were used as test animals, the concentration of the compound in blood was determined after single administration to evaluate the pharmacokinetic behavior.

(164) Experimental Materials:

(165) CD-1 mice (male, 20-40 g, 6-9 weeks, Shanghai Sippr-Bk Laboratory Animal Co., Ltd.)

(166) Experimental Operation:

(167) The pharmacokinetic characteristics of the test compound in rodents after intravenous injection and oral administration were tested by standard protocols, in the experiment, the test compound was prepared into a clear solution or homogeneous suspension, and administered to the mice by single intravenous injection and oral administration. In the intravenous injection group, the menstruum was a certain proportion of ethanol, Cremophor EL and physiological saline solution, the mixture was vortexed to prepare 1 mg/mL clear solution, then filtered with microporous membrane for later use; in the oral administration group, the menstruum was a certain proportion of methyl cellulose solution or certain proportion of methyl cellulose and Tween 80 aqueous solution, the test compound was mixed with the menstruum, and the mixture was vortexed to prepare 10 mg/mL clear or homogeneous suspension for later use. After 2 mg/kg intravenous administration or 100 mg/kg oral administration to the mice, a certain amount of whole blood samples were collected, centrifuged at 3200 g for 10 minutes, the supernatant was separated to obtain the plasma samples, and the samples were diluted multiple times with blank plasma according to actual needs. The plasma sample was added to acetonitrile solution containing the internal standard with a volume 20 times that of the plasma sample to precipitate proteins, and then centrifuged; the supernatant was separated, and water with a volume twice that of the supernatant was added to the supernatant, which was then centrifuged again; the supernatant was collected for analysis; LC-MS/MS analysis method was used to quantitatively analyze the blood drug concentration, and Phoenix WinNonlin software (Pharsight, USA) was used to calculate the pharmacokinetic parameters, such as peak concentration, time to peak concentration, clearance rate, half-life, area under the concentration-time curve, bioavailability, etc.

(168) Experimental Results:

(169) TABLE-US-00017 TABLE 13 Pharmacokinetic properties of the compound of formula (I) in mice Number of the compound Compound of formula (I) 2 mpk intravenous C.sub.0 (nM) 2720 injection T.sub.1/2 (hr) 0.57 Vd.sub.ss (L/kg) 1.54 Cl (mL/min/kg) 41.7 AUC.sub.0-inf (nM .Math. hr) 1438 100 mpk oral C.sub.max (nM) 25550 administration T.sub.max (hr) 0.38 T.sub.1/2 (hr) 0.92 AUC.sub.0-inf (nM .Math. hr) 37567 Bioavailability 52.2%

(170) Among them, C.sub.0 is the initial concentration, T.sub.1/2 is the elimination half-life, V.sub.dss is the steady-state apparent volume of distribution, Cl is the total clearance rate, AUC.sub.0-inf is the area under the plasma drug concentration-time curve from time 0 to infinity, C.sub.max is the peak concentration, T.sub.max is the time to peak concentration.

(171) The results show that the compound of formula (I) of the present disclosure has good pharmacokinetic properties and oral bioavailability in mice.

Experimental Embodiment 7: In Vivo Pharmacokinetic Study of the Crystal Form E of the Compound of Formula (I) in Beagle Dogs After Single Administration

(172) Experimental Objective

(173) Male beagle dogs were used as test animals, the concentration of the compound in blood after single administration was determined to evaluate the pharmacokinetic behavior.

(174) Experimental Materials:

(175) Beagle dogs (Male, 6-12 kg, more than 6 months, Beijing Marshall Biotechnology Co., Ltd.).

(176) Experimental Operation:

(177) The objective of the test was to study the pharmacokinetic characteristics of the test compound in non-rodent after intravenous injection and oral administration. In the experiment, the test compound was prepared into a clear solution or homogeneous suspension and administered to the beagle dogs by single intravenous injection or oral administration. In the intravenous injection group, the menstruum was a certain proportion of dimethyl sulfoxide in HP-β-cyclodextrin solution or a certain proportion of ethanol, polyethylene glycol 400 and physiological saline solution, the mixture was vortexed and subjected to ultrasonic treatment to prepare 2 mg/mL clear solution, then filtered with microporous membrane for later use; in the oral administration group, the menstruum was a certain proportion of dimethyl sulfoxide in HP-β-cyclodextrin solution or a certain proportion of sodium carboxymethyl cellulose solution, the test compound was mixed with the menstruum, vortexed and subjected to ultrasonic treatment to prepare 2 mg/mL homogeneous suspension for later use. After 2 mg/kg intravenous administration or 10 mg/kg oral administration to the Beagle dogs, a certain amount of whole blood samples were collected, centrifuged at 3000 g for 10 minutes, and the supernatant was separated to obtain plasma samples; then an acetonitrile solution containing internal standard with a volume 10 times that of the plasma sample was added to precipitate proteins, and then centrifuged; the supernatant was separated for analysis; LC-MS/MS analysis method was used to quantitatively analyze the blood drug concentration, and Phoenix WinNonlin software (Pharsight, USA) was used to calculate the pharmacokinetic parameters, such as peak concentration, time to peak concentration, clearance rate, half-life, area under the concentration-time curve, bioavailability, etc.

(178) Experimental Results:

(179) TABLE-US-00018 TABLE 14 Pharmacokinetic properties of crystal form E of compounds of formula (I) in dogs Test Compound 2 mpk C.sub.0 (nM) 8463 compound of formula intravenous T.sub.1/2 (hr) 0.44 (I) injection Vd.sub.ss (L/kg) 0.49 Cl 12.4 (mL/min/kg) AUC.sub.0-inf 4664 (nM .Math. hr) Crystal form 10 mpk oral Cmax (nM) 10445 E of the administration T.sub.max (hr) 0.75 compound T.sub.1/2 (hr) 0.95 of formula AUC.sub.0-inf 26362 (I) (nM .Math. hr) Bioavailability 113%

(180) Among them, C.sub.0 is the initial concentration, T.sub.1/2 is the elimination half-life, V.sub.dss is the steady-state apparent volume of distribution, Cl is the total clearance rate, AUC.sub.0-inf is the area under the plasma drug concentration-time curve from time 0 to infinity, C.sub.max is the peak concentration, T.sub.max is the time to peak concentration.

(181) The results show that: the crystal form E of the compound of formula (I) shows a super-linear increase in exposure under low-dose (10 mpk) oral administration, indicating that the compound has good oral pharmacokinetic properties and bioavailability in beagle dogs.