Detergent composition

Abstract

Peptide aldehydes containing a ureido group are effective as stabilizers for protease in a detergent composition, and as stabilizers for a second enzyme in a liquid composition with a protease.

Claims

1. A liquid automatic dishwashing detergent composition comprising a surfactant, a protease and a peptide aldehyde of the formula P-O-A.sup.1-CO-A.sup.2-A.sup.3-A.sup.4-H wherein P is H, Me, Et or CF.sub.3; A.sup.1 is a residue of Tyr, Leu, Phe, Met, Nle or Nva; A.sup.2 is a residue of Gly, Ala, Val, Arg, Leu or Cpd; A.sup.3 is a residue of Val, Gly, Leu or Ile; A.sup.4 is a residue of Phe, Tyr, Leu, Nva, Nle, Met or Arg, wherein CO is a double N-capping group.

2. The composition of claim 1, wherein the peptide aldehyde has the formula Phe-CO-Arg-Val-Phe-H, Phe-CO-Arg-Val-Tyr-H or Phe-CO-Gly-Gly-Tyr-H.

3. The composition of claim 1, which further comprises a second enzyme selected from the group consisting of lipase, amylase, cellulase, and carbohydrase.

4. The composition of claim 1, wherein the protease is an alkaline protease, an aspartic protease, a serine protease, a cysteine protease, or a subtilisin.

5. A liquid enzyme automatic dishwashing detergent composition, comprising a protease at a concentration of at least 0.1% by weight of enzyme protein and a compound of the formula P-O-A.sup.1-CO-A.sup.2-A.sup.3-A.sup.4-H wherein P is H, Me, Et or CF.sub.3; A.sup.1 is a residue of Tyr, Leu, Phe, Met, Nle or Nva; A.sup.2 is a residue of Gly, Ala, Val, Arg, Leu or Cpd; A.sup.3 is a residue of Val, Gly, Leu or Ile; A.sup.4 is a residue of Phe, Tyr, Leu, Nva, Nle, Met or Arg, wherein CO is a double N-capping group.

6. A liquid enzyme automatic dishwashing detergent composition, comprising a protease, a second enzyme and a compound of the formula P-O-A.sup.1-CO-A.sup.2-A.sup.3-A.sup.4-H wherein P is H, Me, Et or CF.sub.3; A.sup.1 is a residue of Tyr, Leu, Phe, Met, Nle or Nva; A.sup.2 is a residue of Gly, Ala, Val, Arg, Leu or Cpd; A.sup.3 is a residue of Val, Gly, Leu or Ile; A.sup.4 is a residue of Phe, Tyr, Leu, Nva, Nle, Met or Arg, wherein CO is a double N-capping group.

7. The composition of claim 1, wherein molar ratio of peptide aldehyde to protease is 0.1:1.

8. The composition of claim 1, wherein molar ratio of peptide aldehyde to protease is 100:1.

9. The composition of claim 1, wherein molar ratio of peptide aldehyde to protease is 0.5:1 to 50:1.

Description

DETAILED DESCRIPTION OF THE INVENTION

(1) Stabilizer

(2) The peptide aldehyde used as a protease stabilizer has the structure PO-(A.sup.i-X).sub.n-A.sup.n+1-H with a double N-capping group XCO, COCO, CS, CSCS or CSCO. It preferably contains four amino acid residues and preferably a ureido group (NHCONH) between two of the residues. Thus, the structure is PO-A.sup.1-CO-A.sup.2-A.sup.3-A.sup.4-H, PO-A.sup.1-A.sup.2-CO-A.sup.3-A.sup.4-H or PO-A.sup.1-A.sup.2-A.sup.3-CO-A.sup.4-H, where the amino acid residue(s) to the right of the ureido group has/have the structure NHCRCO, and the residue(s) to the left of the ureido group is/are inverted to COCRNH.

(3) A.sup.i and A.sup.n+1, most preferably A.sup.1 and/or A.sup.2 and/or A.sup.3 and/or A.sup.4 may be a residue of an amino acid, e.g. an L-amino acid, particularly a naturally occurring amino acid, L-norleucine (Nle), L-norvaline (Nva) or capreomycidine (Cpd). In particular, A.sup.1 may be Tyr, Leu, Phe, Met, Nle or Nva; A.sup.2 may be Gly, Ala, Val, Arg, Leu or Cpd; A.sup.3 may be Ala, Val, Gly, Leu, or Ile; and A.sup.4 may be Phe, Tyr, Leu, Nva, Nle, Met or Arg.

(4) P may be H or the C-terminal protection group Me-, Et- or CF.sub.3

(5) The stabilizer may have the formula HO-A.sup.1-CO-A.sup.2-A.sup.3-A.sup.4-H, e.g. the following compounds (where each amino acid is in the L-form unless indicated):

(6) TABLE-US-00001 Short name A.sup.1 A.sup.2 A.sup.3 A.sup.4 -MAPI Phe Arg Val Phe -MAPI Phe Arg Val D-Phe FCO-RVY-H Phe Arg Val Tyr FCO-GGY-H Phe Gly Gly Tyr FCO-GAF-H Phe Gly Ala Phe FCO-GAY-H Phe Gly Ala Tyr FCO-GAL-H Phe Gly Ala Leu FCO-GA-Nva-H Phe Gly Ala Nva FCO-GA-Nle-H Phe Gly Ala Nle YCO-RVY-H Tyr Arg Val Tyr YCO-GAY-H Tyr Gly Ala Tyr FCS-RVF-H Phe Arg Val Phe FCS-RVY-H Phe Arg Val Tyr FCS-GAY-H Phe Gly Ala Tyr Antipain Phe Arg Val Arg GE20372A Tyr Arg Val Phe GE20372B Tyr Arg Val D-Phe Chymostatin A Phe Cpd Leu Phe Chymostatin B Phe Cpd Val Phe Chymostatin C Phe Cpd Ile Phe

(7) The stabilizer may be prepared by methods known in the art, e.g. as described by R. J. Broadbridge et al., Chem. Commun., 1998, 1449-1450; by P. Page et al., J. Org. Chem., 1999, 64, 794-799; or by I. J. Galpin et al., Int. J. Peptide Protein Res., 23, 1984, 477-486.

(8) The molar ratio of the inhibitor to the protease may be 0.1:1 to 100:1, e.g. 0.5:1-50:1, 1:1-25:1 or 2:1-10:1.

(9) Optional Second Stabilizer

(10) Optionally, the enzyme or detergent composition may comprise one or more other protease stabilizers. Examples are boronic acid derivatives, organic acids, protein and peptide type inhibitors and peptide aldehydes, e.g. as described in WO 1995/029223, WO 1996/041859, WO 2006/045310, WO 2006/045310, WO 2007/113142, WO 2008/116915, WO 2009/095425 (patent application EP 08150977.0), WO 2009/118375 (patent application EP 08153299.6), WO 2007/145963, or WO 2007/141736.

(11) Protease

(12) The protease may be a serine, cysteine or aspartic protease. It may be an alkaline microbial protease, a trypsin-like protease or a subtilisin, e.g. a subtilisin derived from Bacillus. The protease may be subtilisin Novo, subtilisin Carlsberg, subtilisin BPN, subtilisin 309, subtilisin 147 and subtilisin 168 (described in WO 89/06279), trypsin (e.g. of porcine or bovine origin), the Fusarium protease described in WO 89/06270, a protease described in WO 1998/020115, WO 01/44452, WO 01/58275, WO 01/58276, WO 2003/006602, or WO 2004/099401, or it may have an amino acid sequence which is at least 90%, at least 95%, at least 98% or at least 99% identical to one of these.

(13) Examples of commercially available proteases (peptidases) include Kannase Everlase, Esperase, Alcalase, Neutrase, Durazym, Savinase, Ovozyme, Liquanase, Polarzyme, Pyrase, Pancreatic Trypsin NOVO (PTN), Bio-Feed Pro and Clear-Lens Pro (all available from Novozymes A/S, Bagsvaerd, Denmark). Other commercially available proteases include Ronozyme Pro, Maxatase, Maxacal, Maxapem, Opticlean, Properase, Purafect, Purafect Ox and Purafact Prime (available from Danisco Genencor, Gist-Brocades, BASF, or DSM Nutritional Products).

(14) Second Enzyme

(15) In addition to the protease, the liquid composition may optionally comprise one or more other enzymes, e.g. selected among amylases, lipolytic enzymes (particularly lipases), cellulases, mannanases and oxidoreductases.

(16) The amylase may be an alpha-amylase of bacterial or fungal origin, e.g. an alpha-amylase from B. licheniformis, described in GB 1,296,839. Commercially available amylases are Duramyl, Termamyl, Stainzyme, Stainzyme Plus, Termamyl Ultra, Fungamyl and BAN (available from Novozymes A/S) and Rapidase, Maxamyl P, Purastar and Purastar OxAm (available from Gist-Brocades and Danisco Genencor).

(17) The cellulase may be of bacterial or fungal origin. It may be a fungal cellulase from Humicola insolens (U.S. Pat. No. 4,435,307) or from Trichoderma, e.g. T. reesei or T. viride. Examples of cellulases are described in EP 0 495 257. Commercially available cellulases include Carezyme, Celluzyme, Endolase, Celluclean (available from Novozymes), Puradax, Puradax HA, and Puradax EG (available from Danisco Genencor).

(18) The oxidoreductase may be a peroxidase or an oxidase such as a laccase. The peroxidase may be of plant, bacterial or fungal origin. Examples are peroxidases derived from a strain of Coprinus, e.g., C. cinerius or C. macrorhizus, or from a strain of Bacillus, e.g., B. pumilus, particularly peroxidase according to WO 91/05858. Suitable laccases herein include those of bacterial or fungal origin. Examples are laccases from Trametes, e.g., T. villosa or T. versicolor, or from a strain of Coprinus, e.g., C. cinereus, or from a strain of Myceliophthora, e.g., M. thermophila.

(19) The lipolytic enzyme may be a lipase or cutinase of bacterial or fungal origin. Examples include a lipase from Thermomyces lanuginosus (Humicola lanuginosa) described in EP 258 068 and EP 305 216, a Rhizomucor miehei lipase, e.g., as described in EP 238 023, a Candida lipase, such as a C. antarctica lipase, e.g., the C. antarctica lipase A or B described in EP 214 761, a Fusarium oxysporum lipase (WO 98/26057), a Pseudomonas lipase such as a P. pseudoalcaligenes and P. alcaligenes lipase, e.g., as described in EP 218 272, a P. cepacia lipase, e.g., as described in EP 331 376, a P. stutzeri lipase, e.g., as disclosed in BP 1,372,034, a P. fluorescens lipase, a Bacillus lipase, e.g., a B. subtilis lipase (Dartois et al., (1993), Biochemica et Biophysica acta 1131, 253-260), a B. stearothermophilus lipase (JP 64/744992), B. pumilus lipase (WO 91/16422), Penicillium camenbertii lipase (Yamaguchi et al., (1991), Gene 103, 61-67), the Geotrichum candidum lipase (Shimada, Y. et al., (1989), J. Biochem. 106, 383-388), and various Rhizopus lipases such as a R. delemar lipase (Hass, M. J et al., (1991), Gene 109, 117-113), a R. niveus lipase (Kugimiya et al., (1992), Biosci. Biotech. Biochem. 56, 716-719) and a R. oryzae lipase. Additional examples are cutinase from Pseudomonas mendocina (WO 88/09367), cutinase from Fusarium solani pisi (WO 90/09446) and cutinase from Humicola insolens (WO 2001/092502). The lipolytic enzyme may be a lipase variant, e.g. described in WO 2000/060063.

(20) Examples of commercially available lipases include Lipex, Lipoprime, Lipopan, Lipopan F, Lipopan Xtra, Lipolase, Lipolase Ultra, Lipozyme, Palatase, Resinase, Novozym 435 and Lecitase (all available from Novozymes A/S). Other commercially available lipases include Lumafast (Pseudomonas mendocina lipase from Danisco Genencor); Lipomax (Ps. pseudoalcaligenes lipase from Gist-Brocades or Danisco Genencor) and Bacillus sp. lipase from Solvay enzymes. Further lipases are available from other suppliers such as Lipase P Amano (Amano Pharmaceutical Co. Ltd.).

(21) Examples of mannanases include Mannaway (product of Novozymes) and MannaStar (product of Danisco Genencor).

(22) Sequence Identity

(23) The degree of identity between two amino acid sequences is calculated as the number of exact matches in an alignment of the two sequences, divided by the length of the shorter of the two sequences. The result is expressed in percent identity. An exact match occurs when the two sequences have identical amino acid residues in the same positions of the overlap. The length of a sequence is the number of amino acid residues in the sequence.

(24) The alignment of the two amino acid sequences may be determined by using the Needle program from the EMBOSS package (http://emboss.org) version 2.8.0. The Needle program implements the global alignment algorithm described in Needleman, S. B. and Wunsch, C. D. (1970) J. Mol. Biol. 48, 443-453. The substitution matrix used is BLOSUM62, gap opening penalty is 10, and gap extension penalty is 0.5.

(25) Alternatively, the alignment may be done by using the MegAlign program (version 7) developed by DNASTAR Inc., part of the Lasergene suite, based on Hein, J. J. (1990). Unified approach to alignment and phylogenies. In Methods in Enzymology, Vol. 183: pp. 626-645. Using the Jotun Hein Method and the settings GAP PENALTY=11, GAP LENGTH PENALTY=3 for multiple alignments and KTUPLE=2 for pairwise alignments a series of percentage identity values can be calculated.

(26) Detergent Composition

(27) The detergent composition is a liquid composition. In addition to the protease, the composition may further comprise a second enzyme, particularly a lipase, an amylase, a cellulase or a carbohydrase.

(28) The invention is particularly applicable to the formulation of liquid detergents where enzyme stability problems are pronounced. The liquid detergent may be aqueous, typically containing 5-95% water, e.g. 5-15% or 20-70% water and 0-20% organic solvent (hereinafter, percentages by weight).

(29) The detergent comprises a surfactant which may be anionic, non-ionic, cationic, amphoteric or a mixture of these types. The detergent will usually contain 5-30% anionic surfactant such as linear alkyl benzene sulphonate (LAS), alpha-olefin sulphonate (AOS), alcohol ethoxy sulphate (AES) or soap. It may also contain 3-20% anionic surfactant such as nonyl phenol ethoxylate or alcohol ethoxylate.

(30) The pH (measured in aqueous detergent solution) will usually be neutral or alkaline, e.g. 7-10. The detergent may contain 1-40% of a detergent builder such as zeolite, phosphate, phosphonate, citrate, NTA, EDTA or DTPA, or it may be unbuilt (i.e. essentially free of a detergent builder). It may also contain other conventional detergent ingredients, e.g. fabric conditioners, foam boosters, bactericides, optical brighteners and perfumes.

(31) The detergent composition may be a fabric cleaning compositions, hard surface cleansing compositions, light duty cleaning compositions including dish cleansing compositions and automatic dishwasher detergent compositions.

(32) The liquid detergent composition may comprise from about 0.0001% to about 10%, more particularly from about 0.00015% to about 1%, and most particularly from about 0.001% to about 0.1% of the inhibitor

(33) Thus, a stabilized liquid enzyme formulation typically contains 0.5-20% by weight, particularly 1-10% by weight, of enzyme protein (total of protease and optional second enzyme) and 0.01%-10% of the inhibitor, more particularly 0.05-5% by weight and most particularly 0.1%-2% by weight of the inhibitor.

(34) A liquid detergent formulation will typically contain 0.04-400 micromolar enzyme (or 1-10000 mg EP/L), more particularly 0.16-160 micromolar enzyme (4-4400 mg EP/L) and most particularly 0.8-80 (20-2200 mg EP/L) and about 1-20 times more of the inhibitor, most particularly about 1-10 times more of the inhibitor.

(35) The liquid detergent composition may contain water and other solvents as carriers. Low molecular weight primary or secondary alcohols exemplified by methanol, ethanol, propanol, and iso-propanol are suitable. Monohydric alcohols are preferred for solubilizing surfactants, but polyols such as those containing from about 2 to about 6 carbon atoms and from about 2 to about 6 hydroxy groups (e.g., 1,3-propanediol, ethylene glycol, glycerine, and 1,2-propanediol) can also be used. The compositions may contain from about 5% to about 90%, typically from about 10% to about 50% of such carriers.

(36) The detergent compositions herein will preferably be formulated such that during use in aqueous cleaning operations, the wash water will have a pH between about 6.8 and about 11. Finished products are typically formulated at this range. Techniques for controlling pH at recommended usage levels include the use of, for example, buffers, alkalis, and acids. Such techniques are well known to those skilled in the art.

(37) When formulating the hard surface cleaning compositions and fabric cleaning compositions of the present invention, the formulator may wish to employ various builders at levels from about 5% to about 50% by weight. Typical builders include the 1-10 micron zeolites, polycarboxylates such as citrate and oxydisuccinates, layered silicates, phosphates, and the like. Other conventional builders are listed in standard formularies.

EXAMPLES

Example 1

(38) Various peptide aldehydes of the invention were produced by a custom peptide synthesis company, all with a purity >80%. The peptide aldehydes were dissolved in DMSO to a concentration of 10 mg/ml before use.

(39) A model liquid detergent A was prepared for testing the various stabilizers:

(40) TABLE-US-00002 Component % w/w Sodium alkylethoxy sulphate (C9-15, 2EO) 6.0 Sodium dodecyl benzene sulphonate 3.0 Sodium toluene sulphonate 3.0 Oleic acid 2.0 Primary alcohol ethoxylate (C12-15, 7EO) 3.0 Primary alcohol ethoxylate (C12-15, 3EO) 2.5 Ethanol 0.5 Monopropylene glycol 2.0 Tri-sodium citrate 2H.sub.2O 4.0 Triethanolamine 0.4 Protease (Savinase 16 LEX) 0.5 Lipase (Lipex 100 L) 0.5 De-ionized water Ad 100% pH adjusted to 8.5 with NaOH

(41) Further, the following detergents were prepared by adding stabilizers to detergent A:

(42) TABLE-US-00003 Detergent Detergent Stabilizer (from a Molar ratio of inhibitor ID A 10 mg/ml solution) relative to protease B 100 g 1.5 mg FCO-RVF-H 3 C 100 g 2.5 mg FCO-RVF-H 5 D 100 g 5.0 mg FCO-RVF-H 10 E 100 g 1.5 mg FCO-RVY-H 3 F 100 g 2.6 mg FCO-RVY-H 5 G 100 g 5.1 mg FCO-RVY-H 10 H 100 g 1.2 mg FCO-GGY-H 3 J 100 g 2.0 mg FCO-GGY-H 5 K 100 g 3.9 mg FCO-GGY-H 10

(43) The detergents were placed in closed glasses at 35 C. and 40 C. Residual activity of lipase and protease was measured (by comparison to a reference stored at 18 C.) at different times, using standard enzyme analytical methods available at Novozymes (protease measured by hydrolysis of N,N-dimethylcasein at 40 C., pH 8.3 and lipase measured by hydrolysis of p-nitrophenyl valerate at 40 C., pH 7.7).

(44) TABLE-US-00004 Residual % residual activity Residual protease activity lipase activity Detergent 4 weeks 35 C. 1 week 40 C. 1 week 35 C. A (reference) 20 11 3 B (FCO-RVF-H, 3x) 63 22 C (FCO-RVF-H, 5x) 83 51 D (FCO-RVF-H, 10x) 78 69 E (FCO-RVY-H, 3x) 62 55 17 F (FCO-RVY-H, 5x) 81 75 53 G (FCO-RVY-H, 10x) 86 81 69 H (FCO-GGY-H, 3x) 26 17 3 J (FCO-GGY-H, 5x) 31 20 4 K (FCO-GGY-H, 10x) 38 28 5

(45) From this example it is clear that the peptide aldehydes of the invention are very efficient as protease stabilizers and as stabilizers for a second enzyme in the presence of a protease.