Method for preparation of reducible degradable hyperbranched polymeric micelles

Abstract

Provided are a reducible degradable hyperbranched-polymer nanomicelle and a method for preparation thereof and an application thereof. Cystamine and polyethylene glycol diglycidyl ether are polymerized by means of a nucleophilic addition mechanism; in one step, a hyperbranched polymer alternatingly arising from cystamine and polyethylene glycol structural units is synthesized and obtained; then, a hyperbranched nanomicelle is formed by means of self-assembly during the process of dialysis. The hyperbranched-polymer chain segments contain both tertiary aminos and disulfide bond structural units and have pH- and reduction responsiveness, and the hyperbranched three-dimensional cavity structure imparts a drug-carrying ability to the nanomicelle.

Claims

1. A method of preparing reducible degradable hyperbranched polymeric micelles comprising: (1) obtaining cystamine by neutralization reaction of cystamine dihydrochloride with a sodium hydroxide solution, which results in a mixture comprising the cystamine, and then extraction of the cystamine from the mixture comprising the cystamine; (2) conducting a nucleophilic substitution reaction of the cystamine and polyethylene glycol diglycidyl ether to obtain reducible degradable hyperbranched polymers; and (3) dialyzing the reducible degradable hyperbranched polymers to produce the reducible degradable hyperbranched polymeric micelles.

2. The method of claim 1, wherein in step (1): 66.7 mL of 40wt % sodium hydroxide solution and 12.15 g of cystamine dihydrochloride are used, and the reaction is carried out in an ice bath, and the extraction is conducted with a mixture of 50 mL ether and 18 mL tetrahydrofuran.

3. The method of claim 1, wherein in step (2): the polyethylene glycol diglycidyl ether has a molecular weight of 352.

4. The method of claim 1, wherein in step (2): the nucleophilic substitution reaction is conducted in an oil bath at 60 C. for 24 hours.

5. The method of claim 1, wherein in step (2): the nucleophilic substitution reaction is carried out in a solvent of 16 mL deionized water.

6. The method of claim 1, wherein in step (3): the dialyzing is conducted for no less than 48 hours in a dialysis bag with a cutoff molecular weight of 3500.

7. The method of claim 1, wherein in step (2): molar ratio of cystamine to polyethylene glycol diglycidyl ether is set as 3:1, 2:1, 2:2, or 2:3.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is a schematic diagram of the reaction between cystamine and polyethylene glycol diglycidyl ether in the invention.

(2) FIG. 2 are SEM images of the reducible degradable hyperbranched polymeric micelles in the invention; a, b, c and d represent the molar ratio of cystamine to polyethylene glycol diglycidyl ether is 3:1, 2:1, 2:2 or 2:3 respectively in the preparations.

(3) FIG. 3 shows the critical micelle concentration (mg/mL) of the reducible degradable hyperbranched polymeric micelles with a molar ratio of cystamine to polyethylene glycol diglycidyl ether at 2:1 in pH 7.4. Abscissa C is the concentration of polymer micelles.

(4) FIG. 4 shows the particle size changes of the reducible degradable hyperbranched polymeric micelles in 10 mM glutathione solution at different time. The molar ratio of cystamine to polyethylene glycol diglycidyl ether is 2:1 in the sample.

(5) FIG. 5 shows acid-base titration curves for the reducible degradable hyperbranched polymeric micelles in the present invention.

(6) FIG. 6 shows the diameter changes of nano micelles with pH in the solutions. The molar ratio of cystamine to polyethylene glycol diglycidyl ether is 2:1 in the sample.

(7) FIG. 7 shows the cytotoxic column result of the reducible degradable hyperbranched polymeric micelles: The molar ratio of cystamine to polyethylene glycol diglycidyl ether is 2:1 in the sample.

(8) FIG. 8 shows a standard curve of absorbance against methotrexate concentration.

DESCRIPTION OF PREFERRED EMBODIMENTS

(9) The detailed implementation of the invention is further described as follows. The following embodiments are used to illustrate the invention, but not to limit the scope of the invention.

EXAMPLE 1

(10) (1) Preparation of Cystamine

(11) Accurately weighed 12.15 g powdered cystamine dihydrochloride is dissolved in 16 mL deionized water, then 60 mL ether and 24 mL tetrahydrofuran are added under stirring. In the ice bath, 40% NaOH solution (66.7 mL) is added dropwise into the mixture above under magnetic stirring for one hour; the upper organic phase is separated. The lower aqueous phase is extracted with a mixture of 50 mL ether and 18 mL tetrahydrofuran. The organic phase is combined and dried with 4 g NaOH for two hours, after filtration the volatile ether and tetrahydrofuran are removed through evaporation, and finally 6.2 g cystamine is obtained with a yield of 75.5%.

(12) (2) Preparation of Hyperbranched Polymers:

(13) Cystamine 304 mg is dissolved into 8 mL ultrapure water under magnetic stirring till completely dissolved, and polyethylene glycol diglycidyl ether 350 mg with 8 mL of ultrapure water is added into the above solution, the reaction is carried out at 60 C. for 24 hours, the hyperbranched polymer solution CP21 is obtained. The synthesis route of cystamine and polyethylene glycol diglycidyl ether is shown in FIG. 1.

(14) Four kinds of hyperbranched polymers CP31, CP21, CP22 and CP23 with different molar ratios of cystamine to polyethylene glycol diglycidyl ether are provided in Table 1.

(15) TABLE-US-00001 TABLE 1 A list of synthetic formulas for hyperbranched polymers polyethylene glycol diglycidyl Cystamine (Number ether (Number average molecular average molecular Sample ID weight 152) weight 350) CP31 3 (mmol) 1 (mmol) 456 (mg) 350 (mg) CP21 2 (mmol) 1 (mmol) 304 (mg) 350 (mg) CP22 2 (mmol) 2 (mmol) 304 (mg) 700 (mg) CP23 2 (mmol) 3 (mmol) 304 (mg) 1050 (mg)

(16) (3) Preparation of Reducible Degradable Hyperbranched Polymeric Nano Micelles:

(17) The polymer solution obtained in step 2) is poured into a dialysis bag (with a cutoff molecular weight 3500), dialysing 3 days in ultrapure water, and the dialysate is changed every 4 hours, and finally the hyperbranched polymeric nano micelles are obtained. The cutoff molecular weight of dialysis bags can be selected according to the specific use process, usually not less than 3500. The scanning electron microscopy (SEM) photographs of the reducible degradable hyperbranched polymeric micelles are showed in FIG. 2, from a to d, respectively, corresponding to the polymers of CP31, CP21, CP22 and CP23 in Table 1. Obviously, the micelle morphologies are basically spherical, and the particle size distributions are relatively uniform.

(18) The reducible and degradable hyperbranched polymer dry powder can be prepared through freeze drying of the nano micelles solution.

(19) According to the application requirements for the nano particle sizes, nano micelles with different particle sizes can be obtained by controlling the mole ratio of cystamine and polyethylene glycol two glycidyl ether. The nano micelle sample with the molar ratio of cystamine to polyethylene glycol diglycidyl ether at 2:1 has regular sphericity and suitable for applications, and the chemical properties of nano micelles with different particle sizes are same, therefore, it is taken as a representative for the subsequent implementation case unless otherwise stated.

(20) Measurement of Critical Micelle Concentration of the Reducible Degradable Hyperbranched Polymeric Micelles

(21) Solution of reducible degradable hyperbranched polymeric micelles CP21 with a particular concentration are prepared. Then, 30 L of pyrene acetone solution with concentration of 1.62210.sup.5 g/mL is added to 4 mL of the hyperbranched polymeric micelles solution. The solution is oscillated several times till to uniform. After evaporation of acetone the emission spectrum is determined by the fluorescence spectrophotometer, the excitation wavelength is set to 330 nm, the width of the excitation and emission of the slit is 5 nm, and the scanning range is 350500 nm. A curve is drawn by taking the logarithm micelle concentration as X axis and I.sub.1/I.sub.3 as Y axis. As can be seen from FIG. 3, the critical micelle concentration of the micelle CP21 is very low, only 3.98 mg/L, so it has strong anti-dilution ability.

(22) The Reduction Sensitivity of the Reducible Degradable Hyperbranched Polymeric Micelles

(23) The reducible degradable hyperbranched polymeric micelles CP21 prepared in Example 1 are placed in a glutathione solution with a concentration of 10 mmol/L, in the micelles cysteamine: polyethylene glycol diglycidyl ether is 2:1, the particle size change of the micelles at different time is recorded by laser light scattering for observing reduction sensitivity of the micelles.

(24) The results are showed in FIG. 4. The particle size becomes smaller after 6 hours contacting with 10 mmol/L glutathione (GSH) solution, indicating that most of the disulfur bonds break and the micelle structure is destroyed.

(25) The pH Sensitivity of the Reducible Degradable Hyperbranched Polymeric Micelles

(26) (1). The 50 mg dry powder of the reducible degradable hyperbranched polymeric micelles CP21 prepared in Example 1 is dissolved in 5 mL of 150 mmol/L NaCl solution, the pH of the solution is adjusted to pH 2 by 1.0 mol/L HCl solution, and is titrated with 0.1 mol/L NaOH solution. In the titration study 5 mL of 150 mmol/L NaCl solution is used as a control group. As shown in FIG. 5, the NaCl solution has no buffering platform, so it has almost no pH buffering capacity. However, the titration curves for the hyperbranched polymeric micelles solutions decline slowly in the pH range of 7.45, and with the increase of cystamine content, the curve slope is more gentle, the buffer capacity is obviously improved. Therefore, the reducible degradable hyperbranched polymeric micelles have good pH sensitivity.

(27) (2). Taking the reducible degradation of hyperbranched polymer CP21 prepared in Example 1 as an example. The pH value of the aqueous solution is controlled in the range of 212 by adding 0.1 mol/L HCl(aq) or 0.1 mol/L NaOH(aq) solution. The particle sizes and the distributions are determined by DLS. FIG. 6 shows that the particle size changed little under the conditions of extremely alkaline and extremely acidic; but the particle size of the micelles increases from 119 nm to 260.7 nm when the pH changes from neutral pH=7.4 to the lysosomal environment pH=5.0, because the tertiary amino, secondary amino and primary amino groups in the polymer skeleton structure will adsorb protons in large quantities, the hyperbranched polymer is highly positively charged, the internal electrostatic repulsion causes the volume expansion of the particles.

(28) Biocompatibility of the Reducible Degradable Hyperbranched Polymeric Micelles

(29) Taking the reducible degradation of hyperbranched polymer CP21 prepared in Example 1 as an example. The micelle sample CP21 is taken as an example. The RPMI-1640 medium containing 10% fetal bovine serum is employed for incubation. 3T3 and Hela cells are planted on the 96 pore plate (110.sup.4 cells/mL). After incubation at 37 C. for 24 h, the culture solution was abandoned. The micelle solution 100 L with different concentration is added in to the hole, each group contains 6 holes. After incubation at 37 C. for 24 h, the culture solution was abandoned. Then, 20 L MTT solution is added to the hole for additional 4 hours incubation, and the culture solution was abandoned. 150 L DMSO is added to each hole under shaking, the absorbance of the solution is determined by enzyme meter at 570 nm, and the cell viability (%) is calculated.

(30) As shown in FIG. 7, cell viability (%) of 3T3 and Hela cells in different concentrations of the micelle solutions are in the range of 92%110%. The cell viabilities of the two kinds of cells are relatively close under the same conditions.

(31) The cell viability slightly decreased with the increase of micelle concentration, but on the whole the cell viability is more than 90%, which is consistent with biocompatibility standard.

(32) Drug Loading Properties of the Reducible Degradable Hyperbranched Polymeric Micelles

(33) Taking the reducible degradation of hyperbranched polymer CP21 prepared in Example 1 as an example. The micelle sample CP21 is taken as an example.

(34) (1) Preparation of Drug Loaded Micelles:

(35) Taking 20 mL of the hyperbranched polymeric micelle solution with concentration of 0.5 mg/mL, and 10 mL of methotrexate solution with concentration of 0.1 mg/mL is added under magnetic stirring for 24 hours at the room temperature, then the solution is transferred to a dialysis bag (cutoff molecular weight 3500) for dialyzing 24 hours, the dialysate is changed every 3 hours, and the drug loaded micelle is filtered by 0.45 m microporous filter membrane. The product obtained is yellowish.

(36) (2) Drug Loading Rate of Hyperbranched Polymer Micelles:

(37) After freeze drying the drug loaded polymer micelles 5.6 mg are dissolved in DMSO with the aid of ultrasound for one hour, then the solution is fixed in a capacity bottle to 10 mL. The absorbance was measured by ultraviolet photometer monitored at 388 nm, and the drug concentration in DMSO is calculated through the standard curve of methotrexate (FIG. 8). The drug loading rate of the polymer micelles is calculated by the following formula:

(38) $\mathrm{LR}\left(\%\right)=\frac{W_D}{W_S}100\%$
Where W.sub.D is the drug weight loaded in the micelles, mg;
W.sub.S is the weight of micelles before drug loading, mg;

(39) Based on the calculation the drug loading rate to methotrexate is 10.32wt %. It can be seen that the reducible degradable hyperbranched polymeric micelles shows high drug loading rate due to the three-dimensional cavity structure.

(40) The above description is only a preferred method of implementation of the invention, and is not used to limit the invention. It should be noted that, for ordinary technical personnel in the field of technology, some improvements and variations can be made under the technical principles of the invention. These improvements and variations should also be considered as the scope of protection of the invention.