ADIPOSE TISSUE SPLITTING KIT

Abstract

The present invention is a kit to be used for obtaining Adipose tissue-based stem cells, which especially is suitable for obtainment of Adipose tissue-based stem cells, which comprises a reservoir (1) through which the entrance of the adipose tissue, that is taken from a predetermined area of the individual, into the kit; characterized by comprising a first housing (2), which is located on the said reservoir (1) and enables the transmission of the adipose tissue into the kit, a second housing (3), which is located on the said reservoir (1) and which enables the second injector (40) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), a third housing (4) which enables the third injector (50) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), an empty body (6) which is fixed inside the reservoir (1), a first inlet (7) which enables the transmission of the adipose tissue into the body (6) by pushing of the injector, a passage (8) which can rotate its own axis and which enables the circulation of the adipose tissue that is inside the body (6) between a desired inlet inside the body (6) and another inlet which is positioned in a 90 degree angle to this inlet by this rotation movement, at least one cover (10) which enables the said movement of the passage (8) and covers the top of the reservoir (1), a second inlet (12) which comprises a first splitting element (11) that is obtained by alignment of a multiple of cutting elements (10), and a third inlet (14) which comprises at least one second splitting element (13) which enables the adipose tissue split when it enters to and exits from the injector and is obtained by alignment of a multiple of cutting elements (10).

Claims

1. An Adipose tissue splitting kit which comprises at least one reservoir through which the entrance of the adipose tissues used in the obtainment of the Adipose tissue-based stem cells, and which enables mechanical digestion of the said adipose tissue; characterized by comprising: at least one first housing, which is located on the said reservoir, which fits into the inlet of at least one first injector which retains the adipose tissue and which enables the transmission of the adipose tissue inside the said injector; at least one second housing, which is located on the said reservoir in a perpendicular position to the said first housing and which enables the at least one second injector to be positioned outside the reservoir and in a perpendicular position to the reservoir; at least one third housing which is located on the said reservoir in a perpendicular position to the said second housing and which enables the at least one third injector to be positioned outside the reservoir and in a perpendicular position to the reservoir; at least one body which is fixed inside the reservoir by pins that are located inside the said reservoir; at least one first inlet which is located on the said body and which enables the transmission of the adipose tissue to a passage which is located in the body by pushing of the first injector, in which the adipose tissue is located, by being positioned in a manner to be concentric with the first housing that is located in the reservoir; at least one passage which can rotate its own axis and which enables the circulation of the adipose tissue that is taken from the injectors between a desired inlet inside the body and another inlet which is positioned in a 90 degree angle to this inlet by this rotation movement and which lies as L shape inside the body; at least one cover which enables the said movement of the passage by being positioned in a manner to be connected to the passage shape on the body and covers the top of the reservoir; at least one second inlet which comprises at least one first splitting element; and which is located on the said body in a manner to be positioned perpendicular to the said first inlet; which is positioned in a manner to be concentric with the second housing that is located in the reservoir and enables the adipose tissue split when it enters to and exits from the second injector by the virtue of the negative pressure the second injector creates, during the transmission of the adipose tissue from first injector to the second injector; and which is obtained by alignment of a multiple of cutting elements; at least one third inlet which comprises at least one second splitting element; which is located on the said body in a manner to be positioned parallel to the said first inlet, and which is positioned in a manner to be concentric with the third housing which is located in the reservoir; which enables the adipose tissue split when it enters to and exits from the third injector by the virtue of the negative pressure the third injector creates, during the transmission of the adipose tissue from second injector to the third injector; and which has cutting elements located closer to each other in comparison to the cutting elements that are in the said first splitting element.

2. An adipose tissue splitting kit according to claim 1, characterized by comprising at least one forth housing which is located on the said reservoir in a manner to be positioned perpendicular to the said first housing and the third housing and which enables the at least one empty injector be positioned outside the reservoir and in a perpendicular position to the reservoir.

3. An adipose tissue splitting kit according to claim 1, characterized by comprising at least one fourth inlet which is positioned on the body in a manner to be concentric with the said fourth housing.

4. An adipose tissue splitting kit according to claim 1, characterized by comprising at least one third splitting element which is located on the said fourth inlet and which has cutting elements located closer to each other in comparison to the cutting elements that are in the said second splitting element.

5. An adipose tissue splitting kit according to claim 1, characterized by comprising at least one first sealing ring which provides impermeability in order for the adipose tissue not to leak when the said cover gets into the body.

6. An adipose tissue splitting kit according to claim 1, characterized by comprising at least one second sealing ring which enables the cutting elements position on the body.

7. An adipose tissue splitting kit according to claim 1, characterized by comprising at least one orifice which is positioned on the cover which enables the said cover seat on the said reservoir.

8. An adipose tissue splitting kit according to claim 1, wherein said reservoir has a cubic form.

9. An adipose tissue splitting kit according to claim 1, characterized by comprising four pieces of injectors made of metal, into which the obtained adipose tissue-based stem cells are loaded and which are suitable to be used in centrifuge.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0038] FIG. 1 Is an exploded perspective view of the kit that is developed by the present invention.

[0039] FIG. 2 Is a sectional view of the kit with the injectors, that is developed by the present invention.

[0040] FIG. 3 Is a detailed view from bottom of the cover that is comprised in the kit, that is developed by the present invention.

[0041] FIG. 4 Is a detailed perspective view of the body that is comprised in the kit, that is developed by the present invention

[0042] FIG. 5 Is a detailed view from top of the reservoir that is comprised in the kit, that is developed by the present invention.

[0043] It is not necessary that the figures are scaled and the details that are not required in order to understand the present invention may be ignored. In addition to that, elements which are majorly identical or which have majorly identical functions are shown with the same number.

DESCRIPTION OF THE PART REFERENCES

[0044] 1. Reservoir [0045] 2. First housing [0046] 3. Second housing [0047] 4. Third housing [0048] 5. Pin [0049] 6. Body [0050] 7. First inlet [0051] 8. Passage [0052] 9. Cover [0053] 10. Cutting element [0054] 11. First splitting element [0055] 12. Second inlet [0056] 13. Second splitting element [0057] 14. Third inlet [0058] 15. Forth housing [0059] 16. Forth inlet [0060] 17. Third splitting element [0061] 18. First sealing ring [0062] 19. Second sealing ring [0063] 20. Orifice [0064] 30. First injector [0065] 40. Second injector [0066] 50. Third injector [0067] 60. Forth injector

DETAILED DESCRIPTION OF THE INVENTION

[0068] In this detailed description a kit of the invention that is developed for obtaining the Adipose tissue-based stem cells and its preferred structurings are described in order for the issue to be better understood and this description is not limiting in any manner.

[0069] In an example application of the lit which is realized with the present invention and is used for obtaining the Adipose tissue-based stem cells and comprises a reservoir (1), the first injector (30) which refrains the adipose tissue that is taken from a predetermined area of the individual is attached to a first housing (2) which is located on the said reservoir (1) and which is suitable for the inlet of the first injector (30) which refrains the adipose tissue. The adipose tissue which is retained by the First injector (30) is transmitted without the kit having any contact with air or outer environment. There is a third housing (4) which is positioned opposing with the said first housing (2) on the Reservoir (1) and which is positioned in a manner to be perpendicular to a second housing (3). An empty second injector (40) and the third injector (50) are attached to the said housings (3, 4) in a manner to be out of and perpendicular to the reservoir (1). A body (6) which comprises at least one first inlet (7) which enables the transmission of the adipose tissue into the passage (8) inside the body (6); by pushing the first injector (30), which is positioned inside the reservoir (1), in a manner to be concentric with the first housing (2) that is located in the reservoir (1), by the help of the pins (5) that are positioned inside the said reservoir (1) and which comprises the adipose tissue. There is a second inlet (12) comprised, which is positioned on the said body (6) in a manner to be perpendicular to the said first inlet (7) and to be concentric with the second housing (3) that is located on the reservoir (1); and a third inlet (14) which is positioned on the said body (6) in a manner to be perpendicular to the said first inlet (7) and to be concentric with the third housing (4) that is located on the reservoir (1). Because the said inlets are positioned concentrically with the housings, the second inlet (12) is connected to the second injector (40) while the third inlet (14) is connected to the third injector (50).

[0070] There is at least one forth housing (15) which is located on the said reservoir (1) in a manner to be perpendicular to the said first housing (2) and the third housing (4) and to be placed against the said second housing (3) and which enables at least one forth injector (60) to be positioned out of the reservoir (1) in a manner to be perpendicular to the reservoir (1). There is at least one forth inlet (16) which is located in the body (6) in a manner to be concentric with the said forth housing (15). There is a at least one third splitting element (17) comprised, which is located in the said forth inlet (16) and which has cutting elements (10) closer to each other in comparison to the cutting elements in the said second splitting element (13). By this way, the splitting process occurs more efficiently. The forth injector (60) which is located in the said forth housing (15), enables the Adipose tissue split during its entrance to and exit from the forth injector (60), due to the negative pressure it applies, during the transmission of the adipose tissue from third injector (50) to the forth injector (60). It is preferred that the number of the said splitting elements in the invention is at least two.

[0071] There is at least one passage (8) comprised, which lies in L shape inside the body (6), which can rotate on its own axis and which enables by this rotation movement the circulation of the adipose tissue inside it between a desired inlet (7, 12, 14, 16) inside the body (6) and another inlet (7, 12, 14, 16) which is positioned in a 90 degree angle to the said inlet. In the kit that is developed by the present invention, there is a cover (9) comprised which covers the top of the reservoir (1), which is connected to the passage (8) and which enables the passage (8) to rotate around its own axis in a manner to have a 90-degree-angle. Therefore, the said passage (8) can rotate in a manner to adjust the distance between itself and the inlets inside the body (6) as 90 degrees and rotates a full circle inside the body (6) and around its own axis. The injector is drawn by attaching the second injector (40) to the second housing (3) which is positioned on the reservoir (1) which corresponds to the second inlet (12) which is positioned in a 90 degree-angle with the said first inlet (7). Drawing of the said second injector (40) results in the creation of a negative pressure in the passage (8) and the adipose tissue passes from the said first inlet (7) to the second inlet (12). Adipose tissue passes through a first splitting element (11) which is located on the second inlet (12). By drawing and releasing of the said first injector (30) and the second injector (40), it is enabled that the adipose tissue passes through a first splitting element (11) which is located on the second inlet (12) multiple times. Therefore, the adipose tissue passing through the first splitting element (11) starts to split. By the rotation of the cover (9) that is located on the kit, the passage (8) performs the rotation movement of 90 degrees around its own axis in clockwise or counterclockwise direction. Therefore, it is enabled that a passing occurs between the said second inlet (12) and a third inlet (14) that is perpendicular to it. By the virtue of at least one second splitting element (13) which is located on the said third inlet (14) and which has cutting elements (10) located closer to each other in comparison to the cutting elements (10) that are in the said first splitting element (11); by the help of the negative pressure created by the third injector (50), the adipose tissue is transmitted from the second injector (40) to the third injector (50). Therefore, the adipose tissue passes through the said second splitting element (13) and splits. By the developed kit, the adipose tissue is not being pushed inside the kit, but it is drawn by creation of a negative pressure. Therefore, the adipose molecules split by the vacuum effect and by the splitting elements; and the regenerative stem cells are obtained in a pure condition.

[0072] The said adipose cells are loaded into an injector by the rotation of the passage (8) and the cover (9) which are located on the kit. The process is completed by drawing the adipose cells into the first injector (30). Therefore, the adipose tissue, which is loaded into the reservoir (1) without any contact with air, is split by passing through all the said splitting elements (11, 13) by the rotation movement of the cover (9) and the freed stem cells are taken without any contact with air after isolation and are sent to centrifuge in order to be transplanted to the patient

[0073] A preferred implementation of the invention comprises at least one first sealing ring (18) which provides impermeability in order to prevent adipose tissue to leak from the space that occurs when the said cover (9) enters into the body (6).

[0074] Another preferred implementation of the invention comprises at least one second sealing ring (19) which enables the cutting blades seat onto the body (6).

[0075] A preferred implementation of the invention comprises at least one orifice (20) that is located in the cover (9). By the said orifice (20), body (6) seats onto the reservoir (1) well and it is enabled that the passage (8) inside the body (6) easily rotates with the desired 90 degree angle.

[0076] By virtue of the kit that is developed by the present invention, adipose tissue-based stem cells can be obtained without using any enzymes. The said adipose cells are loaded into an injector which is made of a metal suitable to be used in centrifuge, by the rotation of the passage (8) and the cover (9) which are located on the kit. The process is completed by drawing the adipose tissue-based stem cells into the said injector. Therefore, the adipose tissue, which is loaded into the reservoir (1) without any contact with air, is split by passing through all the said splitting elements (11, 13, 17) by the rotation movement of the cover (9) and the obtained stem cells are taken without any contact with air and are sent to centrifuge in order to be transplanted to the patient.