BACTERICIDE COMPOSITION

Abstract

The present invention is a bactericide composition containing: (a) an alkyl or an alkenyl amine oxide having an alkyl group or an alkenyl group with 7 or more and 18 or less carbons; and (b) a mono- or polyoxyalkylene alkyl or alkenyl amine having an alkyl group or an alkenyl group with 8 or more and 24 or less carbons and having an average number of added moles of the oxyethylene group of one or more and 8 or less.

Claims

1. A bactericide composition comprising: (A) an alkyl or an alkenyl amine oxide having an alkyl group or an alkenyl group with 7 or more and 18 or less carbons (hereinafter referred to as a component (a)); and (B) a mono- or polyoxyalkylene alkyl or alkenyl amine having an alkyl group or an alkenyl group with 8 or more and 24 or less carbons and including oxyethylene group as an oxyalkylene group, wherein an average number of added moles of the oxyethylene group is one or more and 8 or less (hereinafter referred to as a component (b)).

2. The bactericide composition according to claim 1, wherein the component (a) is contained in an amount of 0.00005% by mass or more and 5% by mass or less.

3. The bactericide composition according to claim 1, wherein a mass ratio (a)/(b) of the content of the component (a) and the content of the component (b) is 0.001 or more and 10 or less.

4. The bactericide composition according to claim 1, wherein a ratio of the total of the content of the component (a) and the content of the component (b) is 70% by mass or more relative to the total content of surfactants.

5. A method of enhancing a bactericidal effect of a bactericide composition, wherein the bactericidal effect of the bactericide composition is enhanced by adding, to (a) an alkyl or an alkenyl amine oxide having an alkyl group or an alkenyl group with 7 or more and 18 or less carbons, (b) a mono- or polyoxyalkylene alkyl or alkenyl amine having an alkyl group or an alkenyl group with 8 or more and 24 or less carbons and including oxyethylene group as an oxyalkylene group, wherein an average number of added moles of the oxyethylene group is one or more and 8 or less.

6. A sterilization method wherein the bactericide composition according to claim 1 is brought into contact with a hard surface.

7. The sterilization method according to claim 6, wherein the bactericide composition contains the component (a) in an amount of 0.00005% by mass or more and 0.5% by mass or less.

Description

EXAMPLES

[0067] Bactericide compositions shown in Tables 1 to 4 were prepared and used for the following evaluation. All the compositions had a pH at 20 C. of 7. Results are shown in Tables 1 to 4.

(1) 10 mL of LB medium was fed into a tissue culture flask, and bacteria (Staphylococcus aureus or colon bacilli) cultured in a petri dish were picked up with a platinum loop, and fed onto the LB medium.
(2) The flask was cultured at 200 rpm and 37 C. for 16 hours.
(3) The LB medium was moved to a 50 mL-centrifugation tube and centrifuged at 4000 rpm and 15 C. for 10 minutes.
(4) A supernatant of the LB medium was discarded, 10 mL of physiological saline solution was added, and centrifugation was carried out again under the same conditions as in (3).
(5) A supernatant of the physiological saline solution was discarded, physiological saline solution was added, so that an OD 1 bacterial suspension was produced.
(6) The solution produced in (5) was used to produce an OD 0.001 bacterial suspension with 0.5% BSA.
(7) 0.3 mL of the bacterial suspension produced in (6) was added, and uniformly applied and spread out onto a SUS304 plate with 8 cm3 cm0.1 cm.
(8) 0.3 mL of a bactericide composition was added, and uniformly applied and spread out onto the plate prepared in (7), and the plate was allowed to stand for 30 minutes.
(9) The solution remaining on the plate was poured into a swab having 1 mL of LP stop solution sealed therein, and a surface of the plate was swabbed and wiped with the swab.
(10) The solution was taken out from the swab, and serially diluted at intervals of 10 times.
(11) 0.1 mL of the serially-diluted solution was added to a standard agar medium, and applied and spread out with a bacteria spreader.
(12) The medium produced in (11) was cultured at 37 C. for 24 hours, and the colony count thereof was carried out to provide a viable cell count [Log (cfu/mL)].

TABLE-US-00001 TABLE 1 Comparative Example Example 1-1 1-2 1-3 1-4 1-5 1-6 1-7 1-1 1-2 Bactericide Formulation (a) a-1 0.01 0.02 0.01 0.01 0.01 composition (mass %) (b) b-1 0.01 0.01 0.01 0.01 b-2 b-3 0.01 (b) b-1 0.01 Water Balance Balance Balance Balance Balance Balance Balance Balance Balance Total 100 100 100 100 100 100 100 100 100 (a)/(b)(mass ratio) 1 1 Viable cell count (Log(cfu/mL)) 4.96 4.93 4.43 4.43 4.69 4.86 4.87 <1 3.34 (Staphylococcus aureus) Example 1-3 1-4 1-5 1-6 1-7 1-8 Bactericide Formulation (a) a-1 0.01 0.0075 0.00625 0.005 0.00375 0.0025 composition (mass %) (b) b-1 0.0025 0.00375 0.005 0.00625 0.0075 b-2 b-3 0.01 (b) b-1 Water Balance Balance Balance Balance Balance Balance Total 100 100 100 100 100 100 (a)/(b)(mass ratio) 1 3 1.7 1 0.6 0.33 Viable cell count (Log(cfu/mL)) 3.43 3.78 3.13 2.67 2.59 2.53 (Staphylococcus aureus)

TABLE-US-00002 TABLE 2 Comparative Example Example 2-1 2-2 2-3 2-4 2-1 2-2 2-3 Bactericide Formulation (a) a-2 0.01 0.02 0.01 0.01 0.01 0.01 composition (mass %) (b) b-1 0.01 b-2 0.01 b-3 0.01 (b) b-1 0.01 Water Balance Balance Balance Balance Balance Balance Balance Total 100 100 100 100 100 100 100 (a)/(b) (mass ratio) 1 1 1 Viable cell count (Log(cfu/mL)) 4.96 3.29 2.86 3.23 2.08 2.18 1.30 (Staphylococcus aureus)

TABLE-US-00003 TABLE 3 Comparative Example Example 3-1 3-1 3-2 3-3 3-4 Bactericide Formulation (a) a-2 0.06 0.03 0.0225 0.015 0.0066 composition (mass %) (b) b-3 0.03 0.0375 0.045 0.0534 Water Balance Balance Balance Balance Balance Total 100 100 100 100 100 (a)/(b) (mass ratio) 1 0.6 0.33 0.12 Viable cell count (Log(cfu/mL)) 2.81 1.48 <1 1.30 1.65 (colon bacilli)

TABLE-US-00004 TABLE 4 Comparative Example Example 4-1 4-1 Bactericide Formulation (a) a-1 0.06 0.0525 composition (mass %) (b) b-1 0.0075 Water Balance Balance Total 100 100 (a)/(b) (mass ratio) 7 Viable cell count(Log(cfu/mL)) (colon bacilli) 2.37 1.23

[0068] In the tables, the expression <1.0 indicates that the result was a detection limit or less.

[0069] In addition, the components in the tables are as follows. [0070] a-1: lauryl dimethylamine oxide [a compound represented by the general formula (a1), wherein R.sup.1a is a lauryl group, R.sup.2a and R.sup.1a are each a methyl group, and q and p are each 0] [0071] a-2: myristyl dimethylamine oxide [a compound represented by the general formula (a1), wherein R.sup.1a is a myristyl group, R.sup.2a and R.sup.1a are each a methyl group, and q and p are each 0] [0072] b-1: polyoxyethylene hydrogenated tallow amine (average number of added moles: 2.1) [a compound represented by the general formula (b1), wherein R.sup.1b is a hydrogenated tallow alkyl group and m+n is 2.1] [0073] b-2: polyoxyethylene hydrogenated tallow amine (average number of added moles: 8) [a compound represented by the general formula (b1), wherein R.sup.1b is a hydrogenated tallow alkyl group and m+n is 8] [0074] b-3: polyoxyethylene cocoamine (average number of added moles: 2.1) [a compound represented by the general formula (b1), wherein R.sup.1b is a coco alkyl group and m+n is 2.1] [0075] b-1: polyoxyethylene hydrogenated tallow amine (average number of added moles: 20) [a compound represented by the general formula (b1), wherein R.sup.1b is a hydrogenated tallow alkyl group and m+n is 20]