METHOD FOR SEPARATION OF POTATO PROTEINS WITH REDUCED ENZYMATIC ACTIVITY FROM POTATO FRUIT JUICE

Abstract

The present invention concerns a method for separation of (a) potato proteins and optionally insoluble fibers from (b) first salts and phenolic and/or glycoalkaloid compounds in potato fruit juice or a derivative thereof, said method comprising the steps of:

(i) providing a potato fruit juice or a derivative thereof;

(ii) subjecting said potato fruit juice or the derivative thereof to a first cross-flow membrane filtration process resulting in a first permeate and a first retentate;

(iii) adding aqueous diafiltration liquid containing one or more salts to the first retentate and performing a second cross-flow membrane filtration as diafiltration, to create a second permeate being a diafiltrate containing at least a portion of said phenolic and/or glycoalkaloid compounds and salts and a second retentate comprising potato proteins, salts and optionally insoluble fibers;

wherein the pH of the first retentate and the second retentate remains within the range of 4.5 to 8.5 during step (ii) and step (iii), said method further comprising a step of eliminating or reducing enzymatic activity.

Claims

1. A method for separation of (a) potato proteins and optionally insoluble fibers from (b) first salts and phenolic and/or glycoalkaloid compounds in potato fruit juice or a derivative thereof, said method comprising the steps of: (i) providing a potato fruit juice or a derivative thereof, comprising: potato proteins; and one or more first salts; and phenolic and/or glycoalkaloid compounds; and optionally insoluble fibers; (ii) subjecting said potato fruit juice or the derivative thereof to a first cross-flow membrane filtration process wherein water and at least a portion of the first salts and at least a portion of the phenolic and/or glycoalkaloid compounds migrate across the membrane into a first permeate and wherein the potato proteins are retained in a first retentate; (iii) adding aqueous diafiltration liquid containing one or more salts to the first retentate obtained in step (ii) to form a diluted first retentate having a conductivity of between 1 mS/cm and 50 mS/cm and subjecting said diluted first retentate to a second cross-flow membrane filtration as diafiltration, to create a second permeate being a diafiltrate containing at least a portion of said phenolic and/or glycoalkaloid compounds and salts and a second retentate comprising potato proteins, salts and optionally insoluble fibers; wherein the pH of the first retentate and the second retentate remains within the range of 4.5 to 8.5, such as in the range of 5.2 to 7.5, preferably in the range of 4.5 to 6.5, such as in the range of 4.8 to 6.5, or 5.0 to 6.5 during step (ii) and step (iii), said method further comprising a step of eliminating or reducing enzymatic activity by exposing: the first retentate obtained in step (ii) prior to step (iii); or the second retentate obtained in step (iii); or any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii), to pH values between 2.0 and 4.5, such as between 2.0 and 4.0, between 2.0 and 3.75, between 2.0 and 3.5, between 2.0 and 3.2, or between 2.0 and 3.0 in a time and temperature interval sufficient to eliminate or reduce unwanted enzymatic activity of the potato proteins without adversely affecting functionality of the potato proteins.

2. The method according claim 1, wherein the first retentate obtained in step (ii) prior to step (iii), the second retentate obtained in step (iii), or any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii), preferably the second retentate obtained in step (iii), is exposed to: a pH value between 2.0 and 4.5, preferably between 2.0 and 4.0, more preferably between 2.0 and 3.75, even more preferably between 2.0 and 3.5, such as between 2.0 and 3.2, or between 2.0 and 3.0; a temperature in the range of 1-70° C., preferably in the range of 5-60° C., more preferably in the range of 10-55° C., even more preferably in the range of 20-45° C.; and in a time span of up to 240 minutes, preferably between 15 seconds and 120 minutes, more preferably between 5 and 60 minutes, such as about 15, 20, 30 or 45 minutes.

3. The method according to claim 1, wherein the first cross-flow membrane filtration process is an ultrafiltration process.

4. The method according to claim 1, wherein the second cross-flow membrane filtration process is an ultrafiltration process.

5-8. (canceled)

9. The method according to claim 1, further comprising the step of subjecting the second retentate to a fifth membrane filtration process resulting in a fifth retentate, wherein at least a portion of the salts migrate across the membrane into a fifth permeate.

10-13. (canceled)

14. The method according to claim 1, wherein the aqueous diafiltration liquid containing one or more salts contains one or more second salts that are added to the aqueous diafiltration liquid and that are different from the one or more first salts.

15. (canceled)

16. (canceled)

17. The method according to claim 1, further comprising the step of adjusting the pH of said potato fruit juice or the derivative thereof to a pH in the range of 4.5 to 8.5, such as in the range of 5.2 to 7.5, preferably in the range of 4.5 to 6.5, such as 4.8 to 6.5, or 5.0 to 6.5 prior to said first cross-flow membrane filtration process.

18. The method according to claim 1, further comprising the step of adjusting the conductivity of said potato fruit juice or the derivative thereof to a conductivity in the range of 2-500 mS/cm, such as in the range of 1 to 50 mS/cm, 2 to 40 mS/cm, 3 to 30 mS/cm, 3 to 25 mS/cm, 3 to 20 mS/cm, or 5 to 17 mS/cm prior to said first cross-flow membrane filtration process.

19. The method according to claim 1, wherein the potato proteins are selected from the group consisting of patatin, protease inhibitors, lipoxygenase, polyphenol oxidase, acid and alkaline phosphatase or mixtures thereof, preferably patatin or protease inhibitors, or mixtures thereof.

20. The method according to claim 1, wherein the potato fruit juice or the derivative thereof is pretreated by centrifugation and/or filtration to remove insoluble material of a particle size larger than 10 micron prior to said first cross-flow membrane filtration process.

21. (canceled)

22. The method according to claim 1, further comprising the step of continuing the addition of aqueous diafiltration liquid containing one or more salts to the first retentate while continuing the second membrane filtration process in step (iii) until the second retentate contains less than a target amount of the phenolic and/or total glycoalkaloid compounds, whereby the separation of the potato proteins from phenolic and/or total glycoalkaloid compounds has been achieved, wherein the target amount of remaining phenolic and/or total glycoalkaloid compounds in the second retentate corresponds to less than 5000 mg phenolic and/or total glycoalkaloid compounds per kg potato protein on the basis of dry weight, such as less than 4000 mg/kg, less than 3000 mg/kg, less than 2000 mg/kg, less than 1500 mg/kg, less than 1250 mg/kg, less than 1000 mg/kg, less than 750 mg/kg, less than 500 mg/kg, or less than 200 mg phenolic and/or total glycoalkaloid compounds/kg potato protein on the basis of dry weight.

23. (canceled)

24. The method according to claim 9, wherein the fifth retentate is further treated with a sixth cross-flow membrane filtration process whereby the potato protein is concentrated in a sixth retentate and the salts migrate through the membrane to create a sixth permeate.

25-28. (canceled)

29. The method according to claim 1, wherein the conductivity of the first retentate and the second retentate remains within the range of 0.1 to 50 mS/cm, such as in the range of 1 to 40 mS/cm, 3 to 30 mS/cm, 3 to 25 mS/cm, 3 to 20 mS/cm, 4 to 20 mS/cm, 5 to 17 mS/cm, 6 to 25 mS/cm, 7 to 25 mS/cm, or 10 to 25 mS/cm during step (ii) and step (iii).

30-32. (canceled)

33. The method according to claim 1, wherein the absorbance at 600 nm of the first retentate and the second retentate remains within the range of 0.01 to 100, such as in the range of 0.1 to 100, 0.5 to 100, 1.0 to 100, 2.0 to 80, 3.0 to 70, 4.0 to 60, or 5.0 to 50 during step (ii) and (iii).

34. The method according to claim 1, wherein the true protein concentration of the first retentate is in the range of 8 g/L to 180 g/L, such as in the range of 10 g/L to 160 g/L, 12 g/L to 150 g/L, 15 g/L to 150 g/L, 20 g/L to 150 g/L, 25 g/L to 150 g/L, 30 g/L to 150 g/L, 20 g/L to 140 g/L, 20 g/L to 130 g/L, 20 g/L to 120 g/L, 20 g/L to 100 g/L, 20 g/L to 80 g/L, 20 g/L to 60 g/L, or 30 g/L to 60 g/L.

35. The method according to claim 1, wherein the solubility of the protein in the first retentate and the second retentate remains within the range of 50% to 99%, such as in the range of 55% to 98%, 60% to 98%, 65% to 98%, 70% to 98%, 75% to 98%, 80% to 98%, or 85% to 98%, relative to the protein soluble in the potato fruit juice or the derivative thereof.

36. (canceled)

37. The method according to claim 1, wherein the true protein concentration of the second retentate is in the range of 8 g/L to 180 g/L, such as in the range of 10 g/L to 160 g/L, 12 g/L to 150 g/L, 15 g/L to 150 g/L, 20 g/L to 150 g/L, 25 g/L to 150 g/L, 30 g/L to 150 g/L, 20 g/L to 140 g/L, 20 g/L to 130 g/L, 20 g/L to 120 g/L, 20 g/L to 100 g/L, 20 g/L to 80 g/L, 20 g/L to 60 g/L, or 30 g/L to 60 g/L.

38-40. (canceled)

41. The method according to claim 9, wherein the fifth retentate comprises patatin and protease inhibitors, said method comprising a further step of subjecting the fifth retentate to a step of separating patatin from protease inhibitors.

42. The method according to claim 24, wherein the sixth retentate comprises patatin and protease inhibitors, said method comprising a further step of subjecting the sixth retentate to a step of separating patatin from protease inhibitors.

43. The method according to claim 41, wherein the step of separating patatin from protease inhibitors is performed using selective precipitation, membrane separation, adsorption techniques, including expanded bed adsorption, or combinations thereof.

Description

BRIEF DESCRIPTION OF THE FIGURES

[0079] FIG. 1 depicts a flow diagram of a preferred embodiment of the invention wherein potato fruit juice is subjected to a first cross-flow membrane filtration step, resulting in a first permeate and a first retentate comprising potato proteins (step (i) and (ii) of the process according to the invention), and wherein the first permeate is subjected to a third cross-flow membrane filtration step, resulting in a third permeate and a third retentate.

[0080] FIG. 2 depicts a flow diagram of a preferred embodiment of the invention wherein the first retentate obtained in step (ii) is subjected in step (iii) to a second cross-flow membrane filtration step (diafiltration) using a diafiltration liquid and the resulting diafiltrate is subjected to a fourth cross-flow membrane filtration step, resulting in a fourth retentate enriched in phenolic and glycoalkaloid compounds and a fourth permeate comprising salts. The second retentate resulting from the a second cross-flow membrane filtration step (diafiltration) is subjected to a step of inhibiting enzymatic activity using a temporary pH-drop.

[0081] FIG. 3 depicts a flow diagram of a preferred embodiment of the process depicted in FIG. 2 wherein the fourth permeate comprising salts is recycled to the diafiltration liquid.

[0082] FIG. 4 depicts a flow diagram of a preferred embodiment of the process depicted in FIG. 3, wherein the second retentate comprising potato proteins is subjected to a step of inhibiting enzymatic activity using a temporary pH-drop, followed by diafiltration in a fifth cross-flow membrane filtration step resulting in a diafiltrate (fifth permeate) and a fifth retentate comprising potato proteins and by subjecting the fifth retentate to a sixth cross-flow membrane filtration step resulting in a sixth permeate mainly comprising water and a sixth retentate comprising potato proteins.

[0083] FIG. 5 depicts a flow diagram of a preferred embodiment of the process as defined herein wherein the fifth retentate or the sixth retentate comprise patatin and protease inhibitor and wherein the patatin is separated from the protease inhibitor.

[0084] FIG. 6 depicts SDS-PAGE analysis results of potato fruit juice, diafiltration permeates and the retentate after diafiltration obtained using a process according to the invention.

[0085] FIG. 7 depicts esterase activity in potato protein retentate samples that were treated at different pH and temperature using a process according to the invention.

[0086] FIG. 8 depicts SDS-PAGE analysis results of clarified retentate, non-bound potato protein fraction, and bound and subsequently released potato protein fraction obtained using a process according to the invention.

DETAILED DESCRIPTION

[0087] Method for Separation of Potato Proteins from Salts, Phenolic Compounds and/or Glycoalkaloid Compounds

[0088] In a first aspect, the present invention concerns a method for separation of (a) potato proteins and optionally insoluble fibers from (b) first salts and phenolic and/or glycoalkaloid compounds in potato fruit juice or a derivative thereof, said method comprising the steps of: [0089] (i) providing a potato fruit juice or a derivative thereof, comprising: [0090] potato proteins; and [0091] one or more first salts; and [0092] phenolic and/or glycoalkaloid compounds; and [0093] optionally insoluble fibers; [0094] (ii) subjecting said potato fruit juice or the derivative thereof to a first cross-flow membrane filtration process wherein water and at least a portion of the first salts and at least a portion of the phenolic and/or glycoalkaloid compounds migrate across the membrane into a first permeate and wherein the potato proteins are retained in a first retentate; [0095] (iii) adding aqueous diafiltration liquid containing one or more salts to the first retentate obtained in step (ii) to form a diluted first retentate having a conductivity of between 1 mS/cm and 50 mS/cm and subjecting said diluted first retentate to a second cross-flow membrane filtration as diafiltration, to create a second permeate being a diafiltrate containing at least a portion of said phenolic and/or glycoalkaloid compounds and salts and a second retentate comprising potato proteins, salts and optionally insoluble fibers;
wherein the pH of the first retentate and the second retentate remains within the range of 4.5 to 8.5, such as in the range of 5.2 to 7.5, preferably in the range of 4.5 to 6.5, such as in the range of 4.8 to 6.5, or 5.0 to 6.5 during step (ii) and step (iii), said method further comprising a step of eliminating or reducing enzymatic activity by exposing: [0096] the first retentate obtained in step (ii) prior to step (iii); or [0097] the second retentate obtained in step (iii); or [0098] any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii),
to pH values between 2.0 and 4.5, such as between 2.0 and 4.0, between 2.0 and 3.75, between 2.0 and 3.5, between 2.0 and 3.2, or between 2.0 and 3.0 in a time and temperature interval sufficient to eliminate or reduce unwanted enzymatic activity of the potato proteins without adversely affecting functionality of the potato proteins.

[0099] In a preferred embodiment, the process as defined herein is performed at a temperature in the range of 1 to 60° C., such as 5 to 55° C., 10 to 50° C., 15 to 48° C., 12 to 45° C., 18 to 30° C., or 22 to 28° C.

[0100] In another preferred embodiment, the temperature of the first retentate and the second retentate is in the range of 1 to 60° C., such as 5 to 55° C., 10 to 50° C., 15 to 48° C., 12 to 45° C., 18 to 30° C., or 22 to 28° C. during step (ii) and step (iii).

[0101] In a very preferred embodiment, at least part of, more preferably all of the potato proteins remain in solution during the process steps as defined herein.

[0102] In a preferred embodiment, the first permeate from said first cross-flow membrane filtration process is subjected to a third cross-flow membrane filtration process, whereby at least a portion of the salts present in the first permeate migrate across the membrane into a third permeate and wherein the phenolic and/or glycoalkaloid compounds are retained in a third retentate

[0103] In this preferred embodiment, the phenolic and/or glycoalkaloid compounds are separated from the one or more first salts present in the first permeate such that the phenolic and/or glycoalkaloid compounds may be isolated in a purified state or a product enriched in the phenolic and glycoalkaloid compounds is obtained as a product of higher value.

[0104] In another preferred embodiment, the second permeate being a diafiltrate from said second cross-flow membrane filtration process is subjected to a fourth cross-flow membrane filtration process, whereby at least a portion of the salts present in said second permeate being a diafiltrate migrate across the membrane into a fourth permeate and wherein the phenolic and/or glycoalkaloid compounds are retained in a fourth retentate.

[0105] As a further highly important cost-saving step, the salts thus separated from the phenolic and/or glycoalkaloid compounds may then be recycled for use in the diafiltration step (iii). By doing so, there is not only saved a significant amount of water and salts for the process, but there will also be cost savings related to disposure of waste.

[0106] Accordingly, in a very preferred embodiment, the fourth permeate is used, at least in part, as the source of said aqueous diafiltration liquid containing one or more salts in claim 1, step (iii). In another preferred embodiment, the fourth permeate and the third permeate are used, at least in part, as the source of said aqueous diafiltration liquid containing one or more salts in step (iii).

[0107] In another embodiment, the fourth permeate is subjected to a reverse osmosis membrane filtration step or evaporation step and the resulting water is used, at least in part, as the source of the water in the aqueous diafiltration liquid in step (iii).

[0108] As will be appreciated by those skilled in the art, the second cross-flow membrane diafiltration process with this recycle stream can be performed in many ways. When starting the second cross-flow membrane diafiltration process, no fourth permeate is available yet. In a first embodiment, the diafiltration step (iii) starts with aqueous diafiltration liquid without added one or more salts and as soon as the fourth permeate is available from the fourth cross-flow membrane process step, this fourth permeate is recycled and used as aqueous diafiltration liquid in step (iii). In this first embodiment, the one or more salts in the aqueous diafiltration liquid thus solely originate from the one or more first salts. Alternatively, diafiltration step (iii) can be started by using the third permeate as aqueous diafiltration liquid and as soon as the fourth permeate is available from the fourth cross-flow membrane process step, this fourth permeate is recycled and used as aqueous diafiltration liquid. In a second embodiment, the diafiltration step (iii) starts with an aqueous diafiltration liquid containing one or more salts that are one or more second salts that are added to the aqueous diafiltration liquid and that are different from the one or more first salts.

[0109] Continuously adding fresh aqueous diafiltration liquid in step (iii) and recycling fourth and/or third permeate to the aqueous diafiltration liquid may require purging part of the fourth and/or third permeate to avoid buildup of the diafiltration flow. Continuously adding fresh aqueous diafiltration liquid in step (iii) and recycling fourth and/or third permeate to the diafiltration liquid combined with purging part of the fourth and/or third permeate may be advantageous to avoid buildup of too high concentrations of salts in the aqueous diafiltration liquid.

[0110] In a preferred embodiment, the method as defined herein is performed as a continuous process. As will be appreciated by the skilled person, if the method as defined herein is performed as a continuous process, the second cross-flow membrane device is positioned downstream of the first cross-flow membrane device, meaning that steps (ii) and (iii) are necessarily performed in different devices. Accordingly, in this embodiment, the first cross-flow membrane and the second cross-flow membrane can be different. In a preferred embodiment, the first and the second cross-flow membrane filtration processes are ultrafiltration processes, wherein the second cross-flow membrane filtration process is performed as diafiltration.

[0111] In another embodiment, the method as defined herein is performed as a (semi-)batch process wherein steps (ii) and (iii) are subsequently performed using the same cross-flow membrane. In this embodiment, the first and the second cross-flow membrane filtration processes are preferably ultrafiltration processes, wherein the second cross-flow membrane filtration process is performed as diafiltration.

[0112] In yet another embodiment, the method as defined herein is performed as a (semi-)batch process wherein steps (ii) and (iii) are subsequently performed using different cross-flow membranes, meaning that steps (ii) and (iii) are necessarily performed in different devices.

[0113] Accordingly, in this embodiment, the first cross-flow membrane and the second cross-flow membrane can be different. In this embodiment, the first and the second cross-flow membrane filtration processes are preferably ultrafiltration processes, wherein the second cross-flow membrane filtration process is performed as diafiltration.

[0114] In an embodiment, the method as defined herein further comprises the step of subjecting the second retentate to a fifth membrane filtration process resulting in a fifth retentate, wherein at least a portion of the salts migrate across the membrane into a fifth permeate. This embodiment can also be performed as a diafiltration step. Accordingly, in an embodiment, aqueous diafiltration liquid is added to the second retentate to form a diluted second retentate before the fifth membrane filtration process, and the fifth membrane filtration process is performed on the diluted second retentate as a fifth cross-flow membrane filtration as diafiltration, resulting in a fifth permeate being a diafiltrate and the fifth retentate.

[0115] In an embodiment, the membrane in the fifth membrane filtration process is an ultrafiltration membrane. In another embodiment, the membrane in the fifth membrane filtration process is a microfiltration membrane and at least a portion of the salts and the proteins soluble in the second retentate migrate across the microfiltration membrane.

[0116] In an embodiment, the fifth retentate is further treated with a sixth cross-flow membrane filtration process whereby the protein is concentrated in a sixth retentate and the salts migrate through the membrane to create a sixth permeate. The resulting sixth permeate comprising salts can be advantageously used as the aqueous diafiltration liquid in the fifth membrane filtration process, if performed as cross-flow diafiltration process. By doing so, there is not only saved a significant amount of water (and salts) for the process, but there will also be cost savings related to disposure of waste.

Potato Fruit Juice or a Derivative Thereof

[0117] As explained hereinbefore, the term ‘potato fruit juice or derivatives thereof’ means the liquid stream after the (industrial scale) separation of potato starch from potatoes (Solanum tuberosum L.) and any aqueous streams derived from processing of such liquid stream by for example flocculation of contaminants and centrifugation, provided that the ‘derivatives thereof’ still comprise: [0118] protein; and [0119] salts; and [0120] phenolic and/or glycoalkaloid compounds; and [0121] optionally insoluble fibers.

[0122] The potato fruit juice or the derivative thereof that is used as a raw material in the process according to the present invention may be obtained by disintegration, e.g. by grinding, shredding and/or pressing, of the raw potatoes, whereby an aqueous solution comprising protein, salts, insoluble fibers, phenolic compounds and glycoalkaloid compounds is released as a juice, and/or the components may be extracted by addition of water or an aqueous extractant solution in combination with physical disruption of the plant tissue and/or cells. Antioxidants, such as sodium sulfite, sodium metabisulfite and ascorbic acid, may preferably be added prior to or during the procedures performed to produce the potato fruit juice or the derivative thereof. Likewise, it may be preferable to add salts to control conductivity and pH controlling substances such as acids or bases to adjust pH to a preferred value.

[0123] The potato fruit juice may be subjected to various pretreatment procedures creating a derivative of the potato fruit juice, such as deaeration, defoaming, solid-liquid separation (such as filtration, centrifugation, decantation, passing through hydroclyclones), heating or cooling.

[0124] The potato fruit juice or the derivative thereof so obtained may be pretreated in various ways prior to separation according to the invention. Preferably, the potato fruit juice or the derivative thereof is pretreated by centrifugation and/or filtration to remove insoluble material of a particle size larger than 10 micron prior to said first cross-flow membrane filtration process.

[0125] In a preferred embodiment the conductivity of the potato fruit juice or the derivative thereof provided in step (i) is in the range of 2-500 mS/cm, such as in the range of 1 to 50 mS/cm, 2 to 40 mS/cm, 3 to 30 mS/cm, 3 to 25 mS/cm, 3 to 20 mS/cm, or 5 to 17 mS/cm.

[0126] In another preferred embodiment, the method as defined herein further comprises the step of adjusting the conductivity of said potato fruit juice or the derivative thereof to a conductivity in the range of 2-500 mS/cm, such as in the range of 1 to 50 mS/cm, 2 to 40 mS/cm, 3 to 30 mS/cm, 3 to 25 mS/cm, 3 to 20 mS/cm, or 5 to 17 mS/cm prior to said first cross-flow membrane filtration process of step (ii).

[0127] In a preferred embodiment the pH of the potato fruit juice or the derivative thereof provided in step (i) is in the range of 4.5 to 8.5, such as in the range of 5.2 to 7.5. For certain applications, the pH of the potato fruit juice or the derivative thereof provided in step (i) is preferably in the range of 4.5 to 6.5, such as 4.8 to 6.5, or 5.0 to 6.5.

[0128] In another preferred embodiment, the method as defined herein further comprises the step of adjusting the pH of said potato fruit juice or the derivative thereof to a pH in the range of 4.5 to 8.5, such as in the range of 5.2 to 7.5 prior to said first cross-flow membrane filtration process of step (ii). For certain applications, the pH of the potato fruit juice or the derivative thereof is preferably adjusted to a pH in the range of 4.5 to 6.5, such as 4.8 to 6.5, or 5.0 to 6.5 prior to said first cross-flow membrane filtration process of step (ii).

Potato Proteins

[0129] In an embodiment, the potato proteins to be separated are selected from the group consisting of patatin, protease inhibitors, lipoxygenase, polyphenol oxidase, acid and alkaline phosphatase, or mixtures thereof, preferably patatin or protease inhibitors, or mixtures thereof, most preferably patatin and protease inhibitors.

[0130] In an embodiment, the potato protein to be separated is patatin. In another embodiment, the potato protein to be separated is protease inhibitors.

[0131] In a preferred embodiment, said potato proteins, said one or more first salts and said phenolic and/or glycoalkaloid compounds are in solution in said potato fruit juice provided in step (i).

Phenolic and/or Glycoalkaloid Compounds

[0132] As explained hereinbefore, the potato fruit juice or the derivative thereof contains phenolic and/or glycoalkaloid compounds, such as (a) phenolic acids, flavonoids, tannins, stilbenes, lignans, gallic acid and/or catechin and (b) α-solanine and/or α-chaconine.

[0133] In an embodiment, the phenolic compounds to be separated are selected from the group consisting of phenolic acids and flavonoids including flavonols, flavanols, and anthocyanins. In another embodiment, the phenolic compounds to be separated are selected from the group consisting of phenolic acids and flavonoids including flavonols, flavanols, and anthocyanins, and combinations thereof.

[0134] In an embodiment, the phenolic compounds to be separated comprise chlorogenic acids. In another embodiment, the phenolic compounds to be separated comprise catechin.

[0135] In an embodiment, the glycoalkaloid compounds to be separated comprise α-solanine and/or α-chaconine.

[0136] In an embodiment, the phenolic and glycoalkaloid compounds to be separated are selected from the group consisting of (a) phenolic acids and flavonoids, including flavonols, flavanols, and anthocyanins, and (b) α-solanine and α-chaconine, and combinations thereof.

Fibers

[0137] The potato fruit juice or the derivative thereof provided in step (i) optionally contains insoluble fibers as defined hereinbefore.

[0138] In a preferred embodiment, the potato fruit juice or the derivative thereof provided in step (i) contains insoluble fibers at a concentration in the range of 0.1 to 30 mg/ml, based on the dry weight of the insoluble fiber, such as 0.2 to 20 mg/ml, 0.3 to 15 mg/ml, 0.5 to 10 mg/ml, or 1.0 to 5 mg/ml, based on the dry weight of the insoluble fiber.

[0139] The presence of insoluble fibers in the potato fruit juice can be related to its corresponding optical density or absorbance at a wavelength of 600 nm, which is a property that can be easily determined on a sample using spectroscopy.

[0140] In a preferred embodiment, the potato fruit juice or the derivative thereof provided in step (i) contains insoluble fibers and the absorbance at 600 nm is in the range of 0.1 to 50, such as in the range of 0.2 to 45, 0.3 to 40, 0.4 to 35, 0.5 to 30, 0.7 to 25, 0.8 to 20, 0.9 to 15, 1.0 to 15, 1.5 to 15, or 2.0 to 15.

[0141] In an embodiment, more than 80% of said insoluble fibers in the potato fruit juice or the derivative thereof are small enough to pass a 50 micron filter, such as a 40 micron filter, a 30 micro filter, a 20 micron filter, a 10 micro filter, a 5 micron filter, or a 2 micron filter.

[0142] In an embodiment, said insoluble fibers are present in the potato fruit juice in a concentration that results in a pellet when the fruit juice is centrifuged in a tabletop centrifuge at 4000 G for 30 min, wherein said pellet constitutes in the range of 0.1 to 10 vol/vol %, such as 0.1 to 5 vol/vol %, 0.1 to 3 vol/vol %, 0.1 to 2 vol/vol %, 0.1 to 1 vol/vol %, 0.2 to 5 vol/vol %, 0.2 to 3 vol/vol %, 0.3 to 5 vol/vol %, 0.3 to 3 vol/vol %, 0.4 to 5 vol/vol %, 0.4 to 4 vol/vol %, 0.5 to 5 vol/vol %, 0.5 to 4 vol/vol %, or 0.5 to 3 vol/vol %, when the potato fruit juice at a pH of 6 to 7 has been diluted (or concentrated) to a true protein concentration of 5 mg/ml.

First Cross-Flow Membrane Process

[0143] In a preferred embodiment, the first cross-flow membrane filtration process to provide the first permeate and the first retentate according to step (ii) of the process as defined hereinbefore is an ultrafiltration process wherein high molecular weight compounds, such as proteins and optionally insoluble fibers, are retained in the first retentate and low molecular weight substances, such as salts and phenolic and/or glycoalkaloid compounds migrate across the membrane as a first permeate.

[0144] Preferably the pore size of the ultrafiltration membrane is in the range of 5 to 500 kDa, such as 10 to 500 kDa, 10 to 200 kDa, 10 to 100 kDa, 10 to 50 kDa, 30 to 500 kDa, or 50 to 500 kDa.

[0145] More preferably, the pore size of the ultrafiltration membrane is in the range of 10 to 50 kDa, such as about 30 kDa.

[0146] The membrane material may be any kind of membrane suitable for cross-flow filtration, examples of which are polyamide, polysulfone, poyethersulfone, polyvinyldifluoride, polyacrylonitrile, cellulose acetate, permanently hydrophilized polysulfone, permanently hydrophilized polyethersulfone and ceramic membranes. Preferably, the membrane material is regenerated cellulose, polypropylene or polyethylene. Most preferably, the membrane material is selected from the group consisting of polysulfone, poyethersulfone, permanently hydrophilized polysulfone and permanently hydrophilized polyethersulfone.

[0147] In an embodiment, the membrane is a flat sheet or a spiral wound flat sheet membrane.

[0148] In an embodiment, the membrane is a hollow fiber or tubular membrane. In a preferred embodiment, the hollow fiber membrane has an internal diameter in the range of 0.5 to 3.0 mm, such as in the range of 0.6 to 2.5 mm, 0.7 to 2.2 mm, 0.8 to 2.0 mm, 0.9 to 1.8 mm, 1.0 to 1.7 mm, or 1.1 to 1.6 mm.

[0149] In a preferred embodiment, the hollow fiber membrane has a length in the range of 30 to 150 cm, such as in the range of 40 to 140 cm, 50 to 130 cm, 60 to 120 cm, 70 to 115 cm, 80 to 110 cm, or 90 to 110 cm.

[0150] In a preferred embodiment, the hollow fiber membrane material comprises polysulfone, cellulose acetate, ceramic material, polyamide, polyethersulfone or permanently hydrophilized polysulfone or polyethersulfone.

[0151] In a preferred embodiment, the hollow fiber membrane has a pore size in the range of 5 kDa to 500 kDa, such as in the range of 10 kDa to 200 kDa, in the range of 20 kDa to 100 kDa, or in the range of 30 kDa to 75 kDa.

[0152] In a preferred embodiment, the hollow fiber membrane is an inside-out membrane wherein the transmembrane transport of liquid is performed from the inner lumen of the membrane to the outside surface of the membrane.

[0153] Preferably, the conductivity of the first retentate remains within the range of 0.1 to 50 mS/cm, such as in the range of 1 to 40 mS/cm, 3 to 30 mS/cm, 3 to 25 mS/cm, 3 to 20 mS/cm, 4 to 20 mS/cm, 5 to 17 mS/cm, 6 to 25 mS/cm, 7 to 25 mS/cm, or 10 to 25 mS/cm during step (ii) as defined herein. More preferably, the electrical conductivity of the first retentate during the step (ii) remains within the range of 3 to 20 mS/cm.

[0154] Preferably, the temperature of the first retentate is in the range of 1 to 60° C., such as 5 to 55° C., 10 to 50° C., 15 to 48° C., 12 to 45° C., 18 to 30° C., or 22 to 28° C. during step (ii) as defined herein.

[0155] In a preferred embodiment, the absorbance at 600 nm of the first retentate remains within the range of 0.01 to 100, such as in the range of 0.1 to 100, 0.5 to 100, 1.0 to 100, 2.0 to 80, 3.0 to 70, 4.0 to 60, or 5.0 to 50 during step (ii) as defined herein. Since the first retentate is concentrated as compared to the potato fruit juice or the derivative thereof provided in step (i), the absorbance at 600 nm of the first retentate is typically higher than that of the potato fruit juice or the derivative thereof.

[0156] In a preferred embodiment, the true protein concentration of the first retentate is in the range of 8 g/L to 180 g/L, such as in the range of 10 g/L to 160 g/L, 12 g/L to 150 g/L, 15 g/L to 150 g/L, 20 g/L to 150 g/L, 25 g/L to 150 g/L, 30 g/L to 150 g/L, 20 g/L to 140 g/L, 20 g/L to 130 g/L, 20 g/L to 120 g/L, 20 g/L to 100 g/L, 20 g/L to 80 g/L, 20 g/L to 60 g/L, or 30 g/L to 60 g/L.

[0157] In another preferred embodiment, the solubility of the potato protein in the first retentate obtained in step (ii) remains within the range of 50% to 99%, such as in the range of 55% to 98%, 60% to 98%, 65% to 98%, 70% to 98%, 75% to 98%, 80% to 98%, or 85% to 98%, relative to the protein soluble in the potato fruit juice or the derivative thereof.

[0158] In another preferred embodiment, the volume concentration factor of step (ii) is in the range of 0.1 to 20, such as in the range of 0.5 to 18, 2.0 to 17, 2.5 to 16, 3.0 to 15, 3.5 to 14, 4.0 to 13, 4.5 to 12, 5.0 to 11, or 5.0 to 10.

Third and Fourth Cross-Flow Membrane Filtration Processes

[0159] In a preferred embodiment, the third and/or fourth cross-flow membrane filtration processes as defined hereinbefore are nanofiltration processes wherein phenolic and/or glycoalkaloid compounds are retained in the retentate and (first) salts migrate across the membrane as a permeate.

[0160] Preferred materials for the nanofiltration membrane include organic polymers, such as cellulose acetate, polysulfone, poly(ether)sulfone, sulfonated polysulfone, aromatic polyamides, polyimide, poly(piperazine amide) and blends and composites thereof.

[0161] The pore size of the nanofiltration membrane is preferably in the range of 100-1000 Da, such as 200-800 Da, or 300-700 Da. For some applications, the pore size is preferably in the range of 500-800 Da, such as 300-500 D, or 150-300 Da.

[0162] Especially interesting nanofiltration devices for the separation of polyphenols and/or glycoalkaloids are Filmtec NF elements from Dupont, USA, Duracon and Dairy Thin film NF elements from SUEZ Water Technologies & Solutions, USA, NFX, NFW and NFG elements from Synder Filtration, USA, vNF Spiral thin film composite membranes from Alfa Laval Corp, Sweden, and X-Flow hollow fiber nanofiltration elements from Pentair, The Netherlands.

Diafiltration Step

[0163] In a preferred embodiment, the second cross-flow membrane filtration process is an ultrafiltration process.

[0164] In order to optimize the processing logistics, and for some applications the processability of said first retentate, the first retentate obtained in step (ii) may advantageously be stored for several hours before the diafiltration of step (iii) is carried out. Thus, in an embodiment, said first retentate is stored for at least 3 hours, such as at least 6 hours, at least 12 hours, at least 18 hours, at least 24 hours, at least 36 hours, or at least 48 hours before the diafiltration in the second cross-flow membrane filtration step of (iii) is performed. In a further embodiment, the diafiltration in the second cross-flow membrane filtration step (iii) may be partly carried out before the storage of said first retentate.

[0165] In yet a further embodiment, said first retentate obtained in step (ii) may be treated with a preliminary diafiltration step using e.g. water or a low conductivity aqueous solution prior to storage and subsequent diafiltration according to step (iii).

[0166] In an embodiment, the storage temperature of said first retentate is within the range of 1 to 25° C., such as 2 to 20° C., 3 to 15° C., 2 to 12° C., 2 to 10° C., or 2 to 8° C.

[0167] In a further embodiment, the pH of said first retentate during storage is, or is adjusted to, a pH in the range of 4.5 to 5.6, such as 4.5 to 5.4, 4.5 to 5.2, or 4.7 to 5.3.

[0168] The one or more second salts that may be added to the aqueous diafiltration liquid in step (iii) of the method as defined herein may be any water soluble salt of on the one hand one or more of sodium, potassium, ammonium, calcium and magnesium and on the other hand one or more of mineral acids and organic acids.

[0169] In a preferred embodiment, the one or more second salts are selected from sodium, potassium, ammonium, calcium or magnesium salts of a mineral acid or an organic acid.

[0170] Examples of suitable organic acids are formic acid, acetic acid, propanoic acid, citric acid, caprylic acid, lactic acid, gluconic acid, tartaric acid, fumaric acid, butanedioic acid and benzoic acid.

[0171] As explained hereinbefore, the membrane applied in the fourth cross-flow membrane filtration step preferably is a nanofiltration membrane. In order to achieve the highest permeability of the salt through a nanofiltration membrane retaining the phenolic compounds in the retentate, the one or more second salts are preferably chosen from the group of salts constituted by only monovalent inorganic anions and cations, such as sodium chloride, potassium chloride and ammonium chloride.

[0172] However, in certain applications there will be a need to limit process costs and the cost of environmentally friendly waste handling. Likewise, certain factory designs cannot withstand the corrosive nature of e.g. chlorides. For such applications, the one or more salts are preferably chosen from the group of sodium sulfate and potassium sulfate.

[0173] The amount of the one or more (second) salts in (or added to) the aqueous diafiltration liquid determines the conductivity of the diluted first retentate during diafiltration step (iii).

[0174] As explained hereinbefore, the diluted first retentate has a conductivity of between 0.1 mS/cm and 50 mS/cm.

[0175] In a preferred embodiment, the electrical conductivity of the second retentate (or the diluted first retentate) remains within the range of 0.1 to 50 mS/cm, such as in the range of 1 to 40 mS/cm, 3 to 30 mS/cm, 3 to 25 mS/cm, 3 to 20 mS/cm, 4 to 20 mS/cm, 5 to 17 mS/cm, 6 to 25 mS/cm, 7 to 25 mS/cm, or 10 to 25 mS/cm during step (iii).

[0176] More preferably, the electrical conductivity of the second retentate (or the diluted first retentate) during the diafiltration step (iii) remains within the range of 3 to 20 mS/cm.

[0177] The nature of the one or more second salts that may be added to the aqueous diafiltration liquid in step (iii) may also influence the pH of the diluted first retentate and the second retentate.

[0178] Preferably, the temperature of the second retentate (or the diluted first retentate) is in the range of 1 to 60° C., such as 5 to 55° C., 10 to 50° C., 15 to 48° C., 12 to 45° C., 18 to 30° C., or 22 to 28° C. during step (iii) as defined herein.

[0179] In a particular embodiment, the diafiltration in step (iii) is performed first at a relatively low temperature to remove the majority of the phenolic and/or glycoalkaloid compounds followed by diafiltration at a relatively higher temperature to remove the remaining phenolic and/or glycoalkaloid compounds. Thus, in a preferred embodiment the retentate in a first phase of diafiltration remains at a temperature in the range of 1 to 30° C., such as 5 to 28° C., 10 to 25° C., or 15 to 22° C., followed by a second phase of diafiltration wherein the retentate remains at a temperature in the range of 30 to 60° C., such as 30 to 55° C., 35 to 52° C., 38 to 50° C., or 40 to 48° C.

[0180] The addition of the aqueous diafiltration liquid step (iii) is to be performed until enough volume has been added, and subsequently removed via the second permeate, to reach a satisfactory (low) level of phenolic and/or glycoalkaloid compounds in the second retentate.

[0181] In an embodiment, the addition of aqueous diafiltration liquid containing one or more salts to the first retentate is continued while continuing the second membrane filtration process in step (iii) until the second retentate contains less than a target amount of the phenolic and/or total glycoalkaloid compounds, whereby the separation of the potato proteins from phenolic and/or total glycoalkaloid compounds has been achieved, wherein the target amount of remaining phenolic and/or total glycoalkaloid compounds in the second retentate corresponds to less than 5000 mg phenolic and/or total glycoalkaloid compounds per kg potato protein on the basis of dry weight, such as less than 4000 mg/kg, less than 3000 mg/kg, less than 2000 mg/kg, less than 1500 mg/kg, less than 1250 mg/kg, less than 1000 mg/kg, less than 750 mg/kg, less than 500 mg/kg, or less than 200 mg phenolic and/or total glycoalkaloid compounds/kg potato protein on the basis of dry weight.

[0182] In some applications it is further important to reach a lower level of phenolic and/or total glycoalkaloid compounds having a molecular weight below 10 kDa. Thus, in a further embodiment, the target value for phenolic and/or total glycoalkaloid compounds having a molecular weight below 10 kDa remaining in the second retentate of diafiltration step (iii) corresponds to less than 1500 mg/kg, such as less than 1000 mg/kg, less than 750 mg/kg, less than 500 mg/kg, less than 300 mg/kg, less than 200 mg/kg, less than 100 mg/kg, or less than 50 mg phenolic and/or total glycoalkaloid compounds/kg protein on the basis of dry weight.

[0183] In an embodiment, glycoalkaloids are efficiently separated from the proteins and optionally from insoluble fibers present in the potato fruit juice or the derivative thereof. Thus, in an embodiment, diafiltration step (iii) brings the total glycoalkaloids remaining in the second retentate below a target amount corresponding to less than 500 mg/kg, such as less than 300 mg/kg, less than 200 mg/kg, less than 100 mg/kg, or less than 50 mg total glycoalkaloids/kg protein on the basis of dry weight.

[0184] In an embodiment, the addition of aqueous diafiltration liquid in step (iii) is performed continuously and with practically the same flow rate as the diafiltrate is migrating through the second cross-flow membrane. In another embodiment, the addition of aqueous diafiltration liquid in step (iii) is done in small portions, such as portions approximately equal to the retentate volume.

[0185] In an embodiment, the total volume of water added during diafiltration step (iii) is in the range of 4 to 20 times the volume of the first retentate, such as 4 to 15 times, 5 to 12 times, 5 to 10 times, 6 to 10 times, 7 to 9 times, or 5 to 9 times the volume of the first retentate. More preferably, the total volume of water added during diafiltration step (iii) is in the range of 6 to 10 times the volume of the first retentate.

[0186] In a preferred embodiment, diafiltration step (iii) comprises a second phase of diafiltration following the addition of aqueous diafiltration liquid containing one or more salts, wherein the diafiltration is continued with the addition of water without salts. In this way the salt concentration and conductivity of the second retentate (or the diluted first retentate) will gradually decrease until a target value for the conductivity has been reached.

[0187] In an embodiment, the second phase of diafiltration in step (iii) is continued with water and without the addition of salts until the conductivity of the second retentate is less than 10 mS/cm, such as less than 8 mS/cm, less than 5 mS/cm, less than 4 mS/cm, less than 3 mS/cm, less than 2 mS/cm, or less than 1 mS/cm.

[0188] In a preferred embodiment, the absorbance at 600 nm of the second retentate (or the diluted first retentate) remains within the range of 0.01 to 100, such as in the range of 0.1 to 100, 0.5 to 100, 1.0 to 100, 2.0 to 80, 3.0 to 70, 4.0 to 60, or 5.0 to 50 during step (iii) as defined herein.

[0189] In another preferred embodiment, the solubility of the potato protein in the second retentate obtained in step (iii) remains within the range of 50% to 99%, such as in the range of 55% to 98%, 60% to 98%, 65% to 98%, 70% to 98%, 75% to 98%, 80% to 98%, or 85% to 98%, relative to the protein soluble in the potato fruit juice or the derivative thereof.

[0190] In a preferred embodiment, the true protein concentration of the second retentate is in the range of 8 g/L to 180 g/L, such as in the range of 10 g/L to 160 g/L, 12 g/L to 150 g/L, 15 g/L to 150 g/L, 20 g/L to 150 g/L, 25 g/L to 150 g/L, 30 g/L to 150 g/L, 20 g/L to 140 g/L, 20 g/L to 130 g/L, 20 g/L to 120 g/L, 20 g/L to 100 g/L, 20 g/L to 80 g/L, 20 g/L to 60 g/L, or 30 g/L to 60 g/L.

[0191] In another preferred embodiment, the membrane flux during diafiltration step (iii) is higher than 5 liter/m.sup.2/h (LMH), such as higher than 6 LMH, higher than 7 LMH, higher than 8 LMH, higher than 9 LMH, higher than 10 LMH, higher than 11 LMH, higher than 12 LMH, higher than 13 LMH, higher than 14 LMH, higher than 15 LMH, higher than 16 LMH, higher than 17 LMH, or higher than 18 LMH.

[0192] In order to optimize the processing logistics, and for some applications the processability of the retentate resulting after diafiltration step (iii), the second retentate may advantageously be stored for several hours before further processing.

[0193] Thus, in an embodiment, the second retentate resulting after the diafiltration step (iii) is stored for at least 3 hours, such as at least 6 hours, at least 12 hours, at least 18 hours, at least 24 hours, at least 36 hours, or at least 48 hours before further processing is performed.

[0194] In an embodiment, the storage temperature of said second retentate resulting after diafiltration step (iii) is within the range of 1 to 25° C., such as 2 to 20° C., 3 to 15° C., 2 to 12° C., 2 to 10° C., or 2 to 8° C.

[0195] In a further embodiment, the pH of said second retentate resulting after diafiltration step (iii) during storage is, or is adjusted to, a pH in the range of 4.5 to 5.6, such as 4.5 to 5.4, 4.5 to 5.2, or 4.7 to 5.3.

Inactivation of Microbial Growth During Processing

[0196] Potato fruit juice and other extracts of potato proteins may contain a high level of microbes naturally originating from the growth and storage of the potatoes in the soil. It is therefore preferred to add a germicidal step during the process according to the invention. This step may be applied directly to the crude potato fruit juice, or it may preferably be applied during and/or in between the membrane filtration processing steps according to the invention.

[0197] Thus, in an embodiment, the germicidal step is performed on the potato fruit juice or the derivative thereof provided in step (i) prior to step (ii). Before diafiltration in step (iii) and after the concentration in step (ii), the volumetric flow rate is reduced. Accordingly, it may be advantageous to perform the germicidal step just before step (iii). Thus, in a preferred embodiment, the germicidal step is performed on the first retentate provided in step (ii) prior to diluting with the aqueous diafiltration liquid in step (iii).

[0198] Since many proteins are highly sensitive to classical pasteurization by heat treatment, leading to loss of functional properties of the proteins, the germicidal step is performed at a temperature in the range of 2-60° C., such as in the range of 5-55° C., in the range of 8-52° C., in the range of 10-50° C., in the range of 12-45° C., in the range of 12-25° C., or in the range of 12-18° C.

[0199] The germicidal step is applied using high intensity UV light, X-rays, pulsed electric field treatment or high-pressure pasteurization.

[0200] In an embodiment, the germicidal step comprises passing of the potato fruit juice or the derivative thereof through a photo bioreactor illuminating high intensity UV light to the fruit juice. In an embodiment, the UV light has a wavelength primarily in the range of 180-300 nm. In an embodiment, the potato fruit juice or the derivative thereof is illuminated with UV light when passing one or more a spiral-shaped tubes, allowing the passage of the UV light. In an embodiment, the inner diameter of said spiral tubes is in the range of 1-15 mm, such as in the range of 2-12 mm, such as in the range of 3-10 mm, such as in the range of 4-9 mm. In an embodiment, the potato fruit juice or the derivative thereof is passed more than one time through the photo bioreactor.

[0201] In an embodiment, the potato fruit juice or the derivative thereof is passing the photo bioreactor simultaneously with one or more of the membrane filtration steps according to the invention. In an embodiment, the photo bioreactor is inserted into the recirculation loop of one or more of the membrane filtration units applied according to the invention such that the potato fruit juice or the derivative thereof is continuously passing the photo bioreactor.

[0202] In an embodiment, the photo bioreactor is inserted as a shunt to the recirculation loop of one or more of the membrane filtration units applied according to the invention. In an embodiment, the photo bioreactor is inserted as a recirculation unit to the holding tank of one or more of the membrane filtration units applied according to the invention. In an embodiment, the germicidal step using a photo bioreactor is applied to the protein solution after the membrane filtration steps according to the invention. In an embodiment, the O.D.600 nm absorption of the resulting protein solution is in the range of 0.02 to 15.0, such as in the range of 0.05 to 8.0, in the range of 0.1 to 7.0, in the range of 0.5 to 6.0, in the range of 0.8 to 5.0, in the range of 0.9 to 4.0, or in the range of 1.0 to 3.0.

[0203] In an embodiment of the invention the photo bioreactor is substantially equal to the photo bioreactor disclosed in WO2019/057257A1, which is hereby incorporated by reference.

Inactivation of Unwanted Enzymatic Activities

[0204] The inventors have surprisingly found that treatment of: [0205] the first retentate obtained in step (ii) prior to step (iii); or [0206] the second retentate obtained in step (iii); or [0207] any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii),
with acidic pH values for a limited period of time may lead to the preferred inactivation, particularly inactivation of the esterase and lipolytic activity of patatins, in a controlled manner without losing the functionality of the proteins (after raising the pH again). Moreover, the inventors have surprisingly found that this inactivation, particularly inactivation of the esterase and lipolytic activity of patatins, improves the flavor of the mixtures of potato proteins and fibers isolated according to the invention.

[0208] The wording ‘any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii)’ includes at least: [0209] the fifth retentate resulting from the fifth cross-flow membrane filtration step; and [0210] the sixth retentate resulting from the sixth cross-flow membrane filtration step.

[0211] As will be appreciated by those skilled in the art, the wording ‘without adversely affecting functionality of the proteins’ is to be understood as ‘without substantially adversely affecting functionality of the proteins’ because subjecting the proteins to the acidic pH values for a limited period of time may negatively affect at least part of the functionality of the proteins.

[0212] In an embodiment, the first retentate obtained in step (ii) prior to step (iii), the second retentate obtained in step (iii), or any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii), preferably the second retentate obtained in step (iii), exposed to the low pH comprises patatin and the enzymatic activity to be eliminated is the esterase activity of said patatin.

[0213] In an embodiment, the first retentate obtained in step (ii) prior to step (iii), the second retentate obtained in step (iii), or any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii), preferably the second retentate obtained in step (iii), exposed to the low pH comprises one or more polyphenol oxidases and the enzymatic activity to be eliminated is the oxidative activity of said polyphenol oxidase.

[0214] In an embodiment, the first retentate obtained in step (ii) prior to step (iii), the second retentate obtained in step (iii), or any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii), preferably the second retentate obtained in step (iii), exposed to the low pH comprise phospholipase and the enzymatic activity to be eliminated is the phospholipase activity of said phospholipase.

[0215] In an embodiment, the inactivation of enzymatic activity is performed at a temperature in the range of 1-70° C., such as in the range of 1-65° C., 5-60° C., 5-15° C., 10-55° C., 10-15° C., 15-55° C., 18-50° C., 18-48° C., or 20-45° C.

[0216] In an embodiment, the inactivation of enzymatic activity is performed in a time span of at least 15 seconds, such as at least 5 minutes, such as at least 10 minutes, such as at least 30 minutes, such as at least 60 minutes, such as at least 120 minutes, such as at least 240 minutes.

[0217] In a very preferred embodiment, the inactivation of enzymatic activity is performed by exposing the first retentate obtained in step (ii) prior to step (iii), the second retentate obtained in step (iii), or any other aqueous stream comprising potato proteins and optionally insoluble fibers obtained downstream of step (iii), preferably the second retentate obtained in step (iii), to: [0218] a pH value between 2.0 and 4.5, preferably between 2.0 and 4.0, more preferably between 2.0 and 3.75, even more preferably between 2.0 and 3.5, such as between 2.0 and 3.2, or between 2.0 and 3.0; [0219] a temperature in the range of 1-70° C., preferably in the range of 5-60° C., more preferably in the range of 10-55° C., even more preferably in the range of 20-45° C.; and [0220] in a time span of up to 240 minutes, preferably between 15 seconds and 120 minutes, more preferably between 5 and 60 minutes, such as about 15, 20, 30 or 45 minutes.

[0221] In a preferred embodiment, the pH of the thus treated stream is brought back to a value between 4.5 and 8.5, such as between 6 and 8, or between 6.5 and 7.5. As will be appreciated by those skilled in the art, such an additional step is not needed if the step of inactivation of enzymatic activity is performed just before a diafiltration step wherein aqueous diafiltration liquid is added, automatically resulting in an increase of the pH.

[0222] The inactivation of enzymatic activity is preferably performed by exposing the first retentate obtained in step (ii) before diafiltration step (iii) to the low pH. Before diafiltration in step (iii) and after the concentration in step (ii), the volumetric flow rate is reduced, such that the amount of acid needed to reduce the pH is limited. Moreover, by subjecting thus treated retentate to diafiltration in step (iii), the pH can be quickly brought back to a value of 4.5 or higher to avoid overexposure of the proteins.

[0223] More preferably, the inactivation of enzymatic activity is performed by exposing the second retentate resulting from diafiltration step (iii) to the low pH. Since the polyphenols have been sufficiently removed from this stream, the risk of unwanted chemical reactions at acidic pH is eliminated.

[0224] It was further surprisingly found that a controlled and limited proteolysis of the potato proteins isolated according to the invention may lead to inactivation of unwanted enzymatic activity without destroying important functional properties, such as the ability to form food gels.

[0225] Thus, in an embodiment, the inactivation of unwanted enzymatic activity of the potato protein isolated according to the invention is achieved by treatment of a solution of the potato protein, optionally with insoluble fibers, with one or more proteolytic enzymes. In an embodiment, the enzymatic activity of patatin is in part or fully inactivated by the treatment with a protease. In an embodiment, the enzymatic activity of phospholipase is in part or fully inactivated by the treatment with a protease. In an embodiment, the enzymatic activity of polyphenol oxidase is in part or fully inactivated by the treatment with a protease.

[0226] In an embodiment, the one or more proteases are endopeptidase. In an embodiment, the one or more proteases are serine proteases. In an embodiment, the one or more proteases are chosen from the group of trypsin-like, chymotrypsin-like, thrombin-like, elastase-like and subtilisin-like proteases. In an embodiment, the one or more proteases are subtilisin.

Separation of Patatin from Protease Inhibitors

[0227] In a preferred embodiment, the fifth retentate comprises patatin and protease inhibitors, and the method as defined herein comprises a further step of subjecting the fifth retentate to a step of separating patatin from protease inhibitors.

[0228] In another preferred embodiment, the sixth retentate comprises patatin and protease inhibitors, and the method as defined herein comprises a further step of subjecting the sixth retentate to a step of separating patatin from protease inhibitors.

[0229] The step of separating patatin from protease inhibitors is preferably performed using selective precipitation, membrane separation, adsorption techniques, including expanded bed adsorption, or combinations thereof.

Examples

Materials

[0230] Chemicals used in the examples herein e.g. for preparing buffers and solutions are commercial products of at least reagent grade. Water used for conducting the experiments is all de-ionized water.

Potato Fruit Juice and Derivatives Thereof

[0231] Potatoes of the variety Folva were obtained from a local supermarket. The potatoes were washed and their surfaces were dried off before the potatoes were shredded including the peel, while the liberated liquid (juice) concomitantly was separated from the main mass of insolubles using a commercial juicer (Nutrijuicer PRO) without diluting with water. A volume of 10 ml sodium sulphite (10 wt %) per 1000 ml juice was added immediately to the juice and the juice was centrifuged for 5 min at 1430 G to remove any remaining large insoluble particles such as insoluble fibers and starch.

[0232] An amount of 10 kg of potatoes yielded about 4.65 L of centrifuged juice with a pH of 6.2 and a conductivity of 10.7 mS/cm, as measured with a Seven2Go S3 conductivity meter from Mettler Toledo, Switzerland. The so obtained potato fruit juice did not contain visible particles larger than about 10 μm, but nevertheless remained unclear with suspended colloid particles.

Buffer Solutions

[0233] (I) A 10 wt % sodium sulphite buffer solution was prepared by dissolving 10 g of sodium sulphite from Sigma Aldrich USA (cat. No.: 13471) in water to a total volume of 100 ml; [0234] (II) A 0.1 M di-potassium hydrogen phosphate pH 7.0 buffer solution was prepared by dissolving 17.42 g dipotassium hydrogen phosphate from Sigma Aldrich USA (cat.no.: P3786) in 900 ml water followed by adjusting pH to 7.0 with 4 M hydrochloric acid. Finally, water was added to obtain a final volume of 1 L; [0235] (III) A 0.1 M sodium sulfate solution was prepared by dissolving 14.2 g anhydrous sodium sulfate SL 99.8% from Lenzing, Austria, in water to a total volume of 1000 ml; [0236] (IV) A 0.1 M pyrocatechol in 0.1 M di-potassium hydrogen phosphate pH 7.0 buffer solution (for PPO detection) was prepared by dissolving 11.01 g pyrocatechol from Sigma Aldrich, USA (cat.no.: C9510) in 100 ml 0.1 M di-potassium hydrogen phosphate pH 7.0; [0237] (V) 20 mg/ml 1-naphtyl acetate in 2-propanol: 80 mg 1-naphtyl acetate from Sigma, USA (Cat.no.: N8505), 4 ml of 2-propanol from VWR BDH Chemicals (Cat.no.: 20922.364); [0238] (VI) 0.1 M tris pH 7.5: 12.1 g Tris VWR Life Science (Cat.no.: 0826), add 900 ml of water adjust pH to 7.5 with 4% HCl, add water to 1 L; [0239] (VII) Fast Blue BB solution 6 mg/ml in 10% SDS (freshly made, kept in the dark): 30 mg of Fast Blue BB Salt hemi(zinc chloride) from Sigma, USA (Cat.no.: F3378), 5 ml 10% SDS; [0240] (VIII) 0.2 mg/ml 1-naphtyl acetate in 0.1 M tris pH 7.5 (freshly made): 30 ml 0.1 M tris pH 7.5, 0.3 ml 20 mg/ml 1-naphtyl acetate in 2-propanol.

SDS-PAGE Electrophoresis Reagents

[0241] LDS sample buffer, 4X was obtained from Expedeon, USA (Cat.no.: NXB31010) SDS Run buffer, 20×was obtained from Expedeon, USA (Cat.no.: NXB50500) Precast 4-20% gradient gels were obtained from Expedeon, USA (Cat.no.: NXG42012K)

[0242] Instant Blue Coomassie staining solution was obtained from Expedeon, USA (Cat.no.ISB1L).

Assays

[0243] Esterase Activity

[0244] To 3 ml 0.2 mg/ml 1-naphtyl acetate in 0.1 M tris pH 7.5 (fresh made), 0.3 ml potato protein sample is added. The solution is mixed well and incubated for exactly 15 minutes. 0.42 ml of Fast Blue BB solution 6 mg/ml in 10% SDS is added. The resulting solution is mixed well and incubated for exactly 10 minutes.

[0245] A reference sample of 0.3 ml tris buffer is made up (=blind). The samples are analysed at 510 nm (O.D. 510) using a spectrophotometer (BK-UV1800 spectrophotometer, Biobase, China). The 510 nm reading from the blind is subtracted from the 510 nm reading from the potato protein sample. The result is expressed as ‘esterase activity signal’ and is used as a relative measure to compare different samples within the same experiment (i.e. without transforming into a units/mg expression of activity).

SDS-PAGE Electrophoresis

[0246] The samples produced in the examples were analyzed using SDS-PAGE gel electrophoresis to determine the protein composition in each sample. The SDS-PAGE gel electrophoresis was performed using an electrophoresis apparatus and precast 4-20% gradient gels from Expedeon USA (Cat.no.: NXG42012K). The protein samples were mixed with LDS sample buffer and incubated for 10 minutes at 70° C. The samples were applied to a precast gel and the proteins were allowed to run for one hour at 200 V 90 mA in the SDS Run buffer at non-reduced running conditions. The gel was developed in the staining solution for three hours and the protein bands were evaluated by visual inspection or analyzed by scanning densitometry to quantify the amount of specific proteins in the test solutions.

Dry Matter Determination

[0247] A Sartorius moisture analyzer (MA37, Sartorius) was used to determine dry matter in a sample by applying 5-10 mL of a sample to the instrument. The sample was dried at 110° C. until constant weight and the remaining dry matter was determined and calculated by the instrument.

Semi-Quantitative Determination of Polyphenol Oxidase (PPO) Activity

[0248] A sample was mixed with a pyrocathecol solution pH 7.0 at ambient temperature. If the sample contained PPO, the solution changed color from colorless to orange within 15 minutes. The color intensity obtained after 15-minute incubation at room temperature indicates the amount of PPO enzyme activity in the sample. A sample with an unknown amount of enzyme activity may quickly be estimated relative to the activity expressed by a reference sample (e.g. the starting material) and dilutions hereof by visual inspection.

[0249] The procedure was as follows. A solution is prepared by combining 1.95 ml 0.1 M potassium phosphate pH 7.0, 1 ml 0.1 M pyrocatechol in 0.1 M potassium phosphate pH 7.0 and 50 μl of a sample. The resulting solution was mixed well and incubated for 15 minutes at ambient temperature. After 15 minutes, the color intensity was scored relative to a reference sample.

Determination of Total Glycoalkaloids

[0250] A HPLC method for determination of total glycoalkaloids was applied according to V. Alt et al., Optimization of glycoalkaloid analysis for use in industrial potato fruit juice downstreaming, Eng. Life Sci., 2005, 5, pp 562-567. α-solanine (Sigma Aldrich, USA, cat no.: 53757) was used as a reference.

Test for Alkaline Colored Phenolic Compounds

[0251] A semi-quantitative test for the content of complex phenolic compounds based on the development of color in alkaline medium was used. A sample with a volume of 0.5 ml was mixed with 3 ml 1 M sodium hydroxide. The color intensity of a set of samples was inspected visually and assigned a relative score as follows: ++++means very strong color intensity, +++means strong color intensity, ++means clearly visible color intensity, + means weak color intensity, (?) means hardly visible color, ND means no visible color.

True Protein Determination

[0252] True protein concentration was also determined by the method of Kjeldahl using the conversion factor N×6,25. All samples were initially dialyzed against demineralised water in dialysis tubing cellulose membrane (Sigma-Aldrich, USA, cat. No.: D9652) to remove any free amino acids and low molecular weight peptides.

Membrane Filtration

[0253] Membrane filtration test runs were performed using a multipurpose pilot membrane filtration unit (PAN 4010 from Koch Membrane Systems, USA) equipped with the relevant membrane types as indicated in the examples.

Example 1: Ultrafiltration and Diafiltration of Potato Fruit Juice

[0254] In examples 1A and 1B, two separate preparations of 30 L potato fruit juice, produced as described in sections ‘Materials’ and ‘Assays’, were subjected to ultrafiltration without any pretreatment followed by diafiltration using either water (Example 1A) or 0.1 M sodium sulfate (Example 1B) as the diafiltration liquid.

[0255] The procedure was as follows. For both tests a 3 inch ROMICON PM30, 1.1 mm i.d. (Koch Membrane Systems, USA) hollow fiber membrane unit with a cut off of 30 kDa was employed. This unit has a membrane area of 2.3 m.sup.2. The cross flow was set at 34 L/min and the TMP (transmembrane pressure) was regulated to be approximately 1.2 bar. The two 30 L potato fruit juice preparations were each concentrated using ultrafiltration to a retentate volume of 6 L (i.e. the concentration factor was 5×) and the clear yellow/brown permeates were collected. Using the built-in heat exchanger of the equipment the temperature of the retentate was kept in the range of 17-20° C. during the entire ultrafiltration procedure.

[0256] Following the 5×concentration, the two preparations were diafiltered as follows. [0257] 1A) To the retentate was added 6 L water followed by further ultrafiltration to again reduce the retentate volume to 6 L while the corresponding permeate was collected in a separate vessel labelled ‘dia-permeate 1A’. This procedure was repeated to create in total 10 water dia-permeates each of 6 L volume. Following the 10.sup.th collection of permeate with water one portion of 6 L 0.2 M sodium sulfate was added to the retentate and the ultrafiltration was continued to create the 11.sup.th permeate. The permeate flux was measured during each of the 11 diafiltration steps and dia-permeate samples were subjected to a semi-quantitative test for the presence of alkaline colored phenolic compounds as described above; [0258] 1B) To the retentate was added 6 L 0.1 M sodium sulfate followed by further ultrafiltration to again reduce the retentate volume to 6 L while the corresponding permeate was collected in a separate vessel labelled ‘dia-permeate 1B’. This procedure was repeated to create in total 10 sodium sulfate dia-permeates each of 6 L volume. Following the 10.sup.th collection of sodium sulfate permeate, three portions of 6 L water was added to the retentate and the ultrafiltration was continued to create the 11.sup.th-13.sup.th permeate. The permeate flux was measured during each of the 13 diafiltration steps and dia-permeate samples were subjected to a semi-quantitative test for the presence of alkaline colored phenolic compounds as described above.

[0259] The measured average flux values obtained from each dia-permeate is listed in Table 1. As can be seen in Table 1, the flux rates in Example 1A (using water for diafiltration) quickly declines from an initial level at 16 LMH (liter/m.sup.2/h) to 3 LMH with an average for the first 10 dia-permeates of 6.6 LMH. In contrast, the flux rates in example 1B (using 0.1 M sodium sulfate for diafiltration) show an increase from an initial level of 17 LMH to 20 LMH with an average for the first 10 dia-permeates of 18.5 LMH. The productivity of the membrane system employed was thus approximately 2.8 times higher when applying 0.1 M sodium sulfate for diafiltration relative to the use of water.

[0260] The semi-quantitative tests for alkaline colored phenols in the dia-permeates further shows that for both Examples 1A and 1B there is a strongly positive score in the first permeates while the scores for Example 1A dia-permeates gradually become lower than for the Example 1B dia-permeates indicating a gradually declining relative permeability of the phenolic substances when using water for diafiltration relative to 0.1 M sodium sulfate.

[0261] Moreover when changing from water to 0.2 M sodium sulfate for creation of dia-permeate 11A a significant increase in the score is observed, which further indicates that the addition of salts promotes the permeability of the phenolic compounds.

[0262] The flux decline from 20 LMH to 8 LMH in dia-permeates 11B to 13B illustrates that even when shifting to water for diafiltration after thorough diafiltration with sodium sulfate the flux is still dependent on added salts being present in the retentate.

TABLE-US-00001 TABLE 1 Alkaline Dia-permeate Flux, LMH phenol test 1A/1B 16/17  ++++/++++ 2A/2B 13/17  ++++/++++ 3A/3B 9/17  +++/++++ 4A/4B 6/18 +++/+++ 5A/5B 6/18 +++/+++ 6A/6B 4/19  ++/+++ 7A/7B 3/19 ++/++ 8A/8B 3/20 +/+ 9A/9B 3/20 (+)/+ .sup.  10A/10B 3/20 (+)/(+) 11A/11B 12/19   ++/ND 12B 12  ND 13B 8 ND Avg. flux permeates 1-10 Example 6.6/18.5 1A/1B

Example 2: Ultrafiltration and Diafiltration of Potato Fruit Juice with Subsequent Isolation of Polyphenols and Glycoalkaloids

[0263] 30 L potato fruit juice, produced as described in sections ‘Materials’ and ‘Assays’ without any pretreatment to clarify the potato fruit juice, was subjected to ultrafiltration followed by diafiltration. The potato fruit juice, having a true protein concentration of 11.2 g/l, had a pH of 6.0 and a conductivity of 13.3 mS/cm. When the potato fruit juice was diluted with water to a 5 mg/ml true protein concentration and centrifuged in a tabletop centrifuge at 4000 G for 30 minutes, there remained a pellet constituting approximately 1 vol/vol % of the potato fruit juice volume. Accordingly, the potato fruit juice comprised a considerable amount of insoluble fibers. O.D. 600 nm of the potato fruit juice was 1.95 (measured as diluted 3× in 50 mM potassium phosphate, at pH 7.0. The resulting absorbance reading was multiplied by 3).

[0264] The ultrafiltration and diafiltration permeates obtained were mixed and thereafter subjected to nanofiltration for separation of the polyphenols and glycoalkaloids from salts. Hereafter the polyphenols were adsorbed onto an ion exchanger to separate polyphenols and glycoalkaloids.

Ultrafiltration

[0265] The procedure was as follows: a 3 inch ROMICON PM30, 1.1 mm i.d. (Koch Membrane Systems, USA) hollow fiber membrane unit was employed. This unit has a membrane area of 2.3 m.sup.2. The cross flow was set at 34 L/min and the TMP (transmembrane pressure) was regulated to be approximately 1.2 bar.

[0266] The 30 L potato fruit juice was concentrated using ultrafiltration to a retentate volume of 6 L (i.e. the concentration factor was 5×) and the 24 L clear purple/brown permeate (labelled ‘first permeate’) was collected and stored at 2-4° C. until further processing. Using the built-in heat exchanger of the equipment, the temperature of the retentate (labelled ‘first retentate’) was kept in the range of 20-25° C. during the entire ultrafiltration procedure.

[0267] The flux observed in the beginning of the ultrafiltration process was approximately 35 LMH and thereafter gradually decreased to 15 LMH when the retentate was 5× concentrated. There were no signs of membrane fiber clogging due the high content of insoluble material (insoluble fibers).

Diafiltration

[0268] Following the 5×concentration using ultrafiltration, 6 L, of 3 g/L sodium sulfite was added to the first retentate (Sigma Aldrich USA, cat. No.: 13471), conductivity=4.1 mS/cm at 18° C., followed by further ultrafiltration to again reduce the retentate volume to 6 L while the corresponding permeate was collected in a separate vessel labelled ‘diafiltrate’. This procedure was repeated in total 12 times such that in total 72 L diafiltrate was obtained. However, only the first 36 L permeate were collected and stored for the subsequent nanofiltration step. Following the diafiltration with sodium sulfite solution, an additional 3×6 L water was added to remove the sodium sulfite from the retentate. The retentate (5.8 L) after diafiltration was labelled ‘retentate’.

[0269] During diafiltration, a gradual increase of flux was observed and at the final stages the flux was in the range of 25 LMH when a 6 L diafiltration portion was added. This correlated well with a gradual increase of pH in the retentate for each diafiltration step added which initially had a pH of 5.9 and at the end of diafiltration with sodium sulfite had a pH of 8.3.

[0270] Analysis of the first 6×6 L ‘diafiltrate’ for alkaline colored phenolic compounds showed that the concentration of phenolic compounds was very high in the first permeate fractions and then gradually decreased for each 6 L permeate fraction. The sixth permeate fraction was estimated to contain less than 10% phenolic compounds than the first permeate. Also, the majority of the total glycoalkaloids were analyzed to be in the first six permeate fractions, although still a significant concentration was also found in the subsequent fractions.

[0271] A sample of the ‘retentate’ obtained after diafiltration was diluted 20 times in 50 mM potassium phosphate at pH 7.0 and the O.D. 600 of the diluted sample was measured to be 0.47. Thus, the undiluted retentate had an O.D. 600 nm of 20×0.47=9.4. The undiluted retentate had a pH value of 7.8 and a conductivity of 0.8 mS/cm.

[0272] The dry matter concentration of the retentate was measured to be 6.7%, corresponding to a total protein/insoluble fiber product yield of 5.8×67=388.6 g. The true protein content was determined by the Kjeldahl method to be 87% and the total glycoalkaloid content was determined to be 75 mg/kg protein (on a dry matter basis).

[0273] SDS-PAGE analysis of potato fruit juice, diafiltration permeates and the retentate after diafiltration was carried out as described in the ‘Assays’ section. Results are depicted in FIG. 6 (lane 1: potato fruit juice; lanes 2-8: diafiltrates step 1-7; lane 9: retentate after diafiltration). The analysis illustrates that the final protein product in the retentate after diafiltration (lane 9) has substantially the same protein composition as the starting material, i.e. potato fruit juice (lane 1). The analysis further illustrates that only very small amounts of potato protein migrates into the diafiltration permeates.

Nanofiltration and Separation of Polyphenols and Glycoalkaloids by Ion Exchange

[0274] A) The membrane filtration unit was equipped with a spiral wound nanofiltration membrane having an approximate molecular weight cut-off of 600-800 Dalton (Model NFG 2B 2538, Synder Filtration, USA). The ‘first permeate’ and the ‘diafiltrate’ obtained as described above were mixed to create a total volume of 60 L of a clear purple/brown solution containing the polyphenols and glycoalkaloids isolated from the potato fruit juice during ultrafiltration and diafiltration. The resulting solution had a pH of 6.8 and a conductivity of 10.4 mS/cm. 10 L of the mixed solution was then nanofiltered using a cross-flow of 22 L/min and a TMP of 5 bar. The process temperature as maintained at 20-25° C. Samples of the permeate and the corresponding retentate were collected when 2 L permeate had been produced, were analyzed for conductivity and were tested for alkaline colored phenolic compounds as described in the ‘Assays’ section. The nanofiltration process was conducted until the 10 L mixed solution was concentrated to about 3 L and then stopped. [0275] The conductivity of the collected nanofiltration permeate and retentate samples were found to be approximately 6.6 mS/cm and 10.9 mS/cm, respectively. This indicates that most of the salts present in the mixture passed the nanofiltration membrane into the permeate. The semi-quantitative test for alkaline colored phenolic compounds indicated that approximately 65% of the alkaline colored phenolic compounds were retained in the retentate. [0276] (B) The membrane filtration unit was then equipped with a spiral wound nanofiltration membrane having an approximate molecular weight cut-off of 300-500 Dalton (Model NFW 2B 1812F, Synder Filtration, USA), and another portion of 10 L of the mixed solution was then nanofiltered using a cross-flow of 6 L/min and a TMP of 5 bar. The process temperature was maintained at 20-25° C. Samples of the permeate and the corresponding retentate were collected when 2 L permeate had been produced and were analyzed for conductivity, alkaline colored phenolic compounds and total glycoalkaloids as described in the ‘Assays’ section. The nanofiltration process was conducted until the 10 L mixed solution was concentrated to about 2 L and then 2 L water was added with subsequent reconcentration to 2 L retentate. This was repeated once to obtain in total 12 L nanofiltration permeate. [0277] The conductivity of the collected nanofiltration permeate and retentate samples were found to be approximately 4 mS/cm and 11.2 mS/cm, respectively. This indicates that approximately one third of the salts present in the mixture passed the membrane into the permeate. A significant additional part of the remaining salts were found to pass the membrane during the 2 diafiltration steps with water, which indicates that the polyphenols and total glycoalkaloids can be efficiently separated from the salts in the mixed solution. The semi-quantitative test for alkaline colored phenolic compounds as well as the test for total glycoalkaloids indicated that no detectable amounts of the alkaline colored phenolic compounds nor total glycoalkaloids were passing the membrane which indicated that this nanofiltration membrane retains approximately 100% of the alkaline colored phenolic compounds and the total glycoalkaloids in the retentate. [0278] The quality of the obtained permeate enables recycling of the permeate and thereby re-use of the water and salts as diafiltration liquid in the diafiltration step to create the ‘diafiltrate’ after 5×concentration of the potato fruit juice as described above. In this way the total water and salt usage of the process according to the invention will be highly reduced.

Separation of Phenolic Compounds and Total Glycoalkaloids by Ion Exchange

[0279] The partly desalted nanofiltration retentate from experiment (B) as described above was applied to a chromatographic column packed with Q Sepharose Fast Flow (Cat no. Q1126, Sigma Aldrich, USA) which was pre-washed and equilibrated with water. The pH of the nanofiltration retentate sample was 6.8. The sample was loaded until the column was nearly completely saturated with the bound phenolic compounds. The run-through (non-bound fraction) from the column was collected and analyzed for the content of alkaline colored phenolic compounds.

[0280] The phenolic compounds were found to bind strongly to the Q Sepharose Fast Flow adsorbent, which could be observed by visual inspection of the column in the form of a dark-brown band of bound phenols that appeared at the top and gradually filled the column as more sample was applied. The collected run-through was negative in the test for alkaline colored phenolic compounds, which indicates that substantially all phenolic compounds were bound the adsorbent. While the phenolic compounds can be negatively charged at near-neutral to alkaline pH values, the glycoalkaloids will be neutral or slightly positively charged and do therefore not bind to the adsorbent. Accordingly, they will migrate in the run-through fraction, and are thus separated from the phenolic compounds. The bound phenolic compounds could be efficiently released from the adsorbent with 0.1 M sodium hydroxide.

Example 3: Ultrafiltration and Diafiltration of Potato Fruit Juice with an Integrated Rated Step for Inactivation of Enzymatic Activity

[0281] 30 L potato fruit juice, produced as described in sections ‘Materials’ and ‘Assays’ without any pretreatment, was subjected to ultrafiltration and diafiltration to clarify the potato fruit juice. The potato fruit juice, having a true protein concentration of 11.5 g/l, had a pH of 6.0 and a conductivity of 13.1 mS/cm. When the potato fruit juice was diluted with water to a 5 mg/ml true protein concentration and centrifuged in a tabletop centrifuge at 4000 G for 30 min there remained a pellet constituting approximately 1 vol/vol % of the potato fruit juice volume. Accordingly, the potato fruit juice comprised a considerable amount of insoluble fibers. O.D. 600 nm of the potato fruit juice was 2.0 (measured as diluted 3× in 50 mM potassium phosphate, at pH 7.0. The resulting absorbance reading was multiplied by 3).

[0282] The retentate obtained after a first diafiltration step was subjected to an enzyme inactivation step, followed by further diafiltration, solubilization and final purification of the resulting protein product.

Ultrafiltration

[0283] The procedure was as follows: a 3 inch ROMICON PM30, 1.1 mm i.d. (Koch Membrane Systems, USA) hollow fiber membrane unit was employed. This unit has a membrane area of 2.3 m.sup.2. The cross flow was set at 34 L/min and the TMP (transmembrane pressure) was regulated to be approximately 1.2 bar.

[0284] The 30 L potato fruit juice was concentrated using ultrafiltration to a retentate volume of 6 L (i.e. the concentration factor was 5×) and the 24 L clear purple/brown permeate (labelled ‘first permeate’) was collected and stored at 2-4° C. until further processing. Using the built-in heat exchanger of the equipment the temperature of the retentate (labelled ‘first retentate’) was kept in the range of 20-25° C. during the entire ultrafiltration procedure.

[0285] The flux observed in the beginning of the ultrafiltration process was approximately 35 LMH and thereafter gradually decreased to 15 LMH when the retentate was 5× concentrated.

[0286] There were no signs of membrane fiber clogging due the high content of insoluble material (insoluble fibers).

Diafiltration

[0287] Following the 5×concentration using ultrafiltration, 6 L, 3 g/L sodium sulfite was added to the first retentate (Sigma Aldrich USA, cat. No.: 13471), conductivity=4.1 mS/cm at 18° C., followed by further ultrafiltration to again reduce the retentate volume to 6 L while the corresponding permeate was collected in a separate vessel labelled ‘diafiltrate’. This procedure was repeated in total 6 times such that in total 36 L diafiltrate was obtained. The retentate was labelled ‘retentate’.

[0288] A sample of the ‘retentate’ obtained after diafiltration was diluted 20 times in 50 mM potassium phosphate at pH 7.0 and the O.D. 600 of the diluted sample was measured to be 0.6. Thus, the undiluted retentate had an O.D. 600 nm of 20×0.6=12. The undiluted retentate had a pH value of 7.7.

[0289] During diafiltration a gradual increase of flux was observed and at the final stages the flux was in the range of 22 LMH when a 6 L diafiltration portion was added. This correlated well with a gradual increase of pH in the retentate for each diafiltration step added which initially had a pH of 5.9 and at the end of this first diafiltration with sodium sulfite had a pH of 7.7.

[0290] Analysis of the first 6×6 L diafiltration permeate for alkaline colored phenols showed that the concentration of phenols was very high in the first permeate fractions and then gradually decreased for each 6 L permeate fraction. The sixth permeate fraction was estimated to contain less than 10% of the initial permeates. Also, the majority of the glycoalkaloids were analyzed to be in the first six permeate fractions, although still a significant concentration was also found in the subsequent fractions.

Inactivation of Enzyme Activity, Further Diafiltration, pH Adjustment and Final Diafiltration

[0291] A 20 ml sample of the retentate obtained after the first diafiltration step was divided into two aliquots which were adjusted with sulfuric acid to a pH of 4.0 and 3.0, respectively. The two solutions were then again divided into two aliquots which were incubated at room temperature (RT, 20-23° C.) and 40° C., respectively, for 3 hrs. During the incubation, samples for analysis of esterase activity were withdrawn, neutralized with sodium hydroxide and analyzed for esterase activity as described in the ‘Assays’ section.

[0292] FIG. 7 shows the esterase activity signal (O.D.510 nm) in the different samples as a function of incubation time at pH 4.0 and pH 3.0, respectively.

[0293] FIG. 7 illustrates that the esterase activity in the retentate sample is reduced when incubated at pH 4.0 or 3.0, respectively, at room temperature. At pH 3, the esterase activity signal at 510 nm decreases strongly over time and after 3 hrs., it is reduced with a factor of 24. When the temperature is increased to 40° C., the inactivation is very effective even after 15 minutes, after which the esterase activity is reduced with a factor of 98. At room temperature and pH 3.0, it is observed that an activity drop of about 85% is reached after about 1 hour and that this level is rather constant until up to about 2 hours after incubation. From a process robustness perspective, it may be preferable to have ample time to perform the operations without changes of the process outcome and it was therefore chosen to perform the next preparative steps at pH 3.0 and room temperature.

Preparative Inactivation Procedure and Subsequent Solubilization

[0294] The retentate after diafiltration was kept in the membrane filtration unit and 6 L, 20 mM sodium sulfate was added (SL 99.8% from Lenzing, Austria adjusted to pH 3.3 with sulfuric acid, conductivity=3.8 mS/cm at 20° C.). Thereafter, the pH was adjusted to 3.0 with sulfuric acid while recirculating in the membrane filtration unit using the built-in heat exchanger to keep the temperature in the range of 20-25° C. Following recirculation for 15 minutes, the retentate was then again concentrated to a volume of 6 L retentate. The permeate was collected and labelled ‘acidic permeate’ and stored at 2-4° C. Following the concentration, again 6 L, 20 mM sodium sulfate, pH 3.3, was added to the retentate. This procedure was repeated in total 6 times such that in total 42 L acidic permeate was obtained.

[0295] To the retentate was then added 6 L water and the pH was adjusted to pH 8.5 with sodium hydroxide while the retentate was kept in the membrane filtration under recirculation. A total of 2 hours at 20-25° C. was passing from the time the retentate was adjusted to pH 3.0 and until the pH was raised again to pH 8.5. The retentate was then again concentrated to a volume of 6 L retentate. The permeate was collected and labelled ‘final permeate’ and stored at 2-4° C. Following the concentration, 6 L of water was added to the retentate. This procedure was repeated in total 3 times such that in total 24 L final permeate was obtained. The retentate was collected from the membrane filtration unit in a volume of 5.8 L and labelled ‘final retentate’.

[0296] The conductivity of the final retentate was 0.8 mS (at 20° C.). The pH was 7.9. The dry matter concentration of the final retentate was 6.5%, corresponding to a total yield of dry matter of 377 g. The protein purity was determined by Kjeldahl analysis as described in the methods section to be 89% and the total glycoalkaloid content was determined to be 25 mg/kg protein (on a dry matter basis). Testing for alkaline colored phenolic compounds gave no visible reaction, indicating that practically all phenolic compounds were removed.

[0297] When the final retentate was diluted to a true protein concentration equal to the potato fruit juice (11.5 g/L) and was analyzed for esterase activity as described in the ‘Assays’ section, it was found that the activity signal was reduced with approximately 85%. Likewise, when performing the determination of polyphenol oxidase (PPO) activity no visible reaction was observed, indicating that practically all the PPO activity was eliminated.

[0298] A sample of the final retentate was diluted 20 times in 50 mM potassium phosphate at pH 7.0 and the O.D. 600 of the diluted sample was measured to be 0.55. Thus, the undiluted final retentate had an O.D. 600 nm of 20×0.55=11. Accordingly, the final retentate comprised a considerable amount of insoluble fibers.

[0299] When diluted to a dry matter concentration of 0.5% in water and after centrifugation for 30 minutes at 4000 G, there was less than 1 vol/vol % pellet. The dry matter of the supernatant was still close to 0.5% indicating a more than 90% solubility of the potato protein product. This is in sharp contrast to prior art reports indicating that low pH treatment of potato fruit juice leads to significant loss of solubility, and thereby functionality.

Example 4: Ultrafiltration and Diafiltration of Potato Fruit Juice Followed by Separation of Patatin and Protease Inhibitors

[0300] 30 L potato fruit juice, produced as described in sections ‘Materials’ and ‘Assays’ without any pretreatment to clarify the potato fruit juice, was subjected to ultrafiltration and diafiltration. The potato fruit juice, having a true protein concentration of 11.3 g/l, had a pH of 6.0 and a conductivity of 13.3 mS/cm. When the potato fruit juice was diluted with water to a 5 mg/ml true protein concentration and was centrifuged in a tabletop centrifuge at 4000 G for 30 minutes, there was a pellet constituting approximately 1 vol/vol % of the fruit potato juice volume. Accordingly, the potato fruit juice comprised a considerable amount of insoluble fibers. O.D. 600 nm of the fruit juice was 2.1 (measured as diluted 3× in 50 mM potassium phosphate, at pH 7.0. The resulting absorbance reading was multiplied by 3).

[0301] The retentate obtained after a first diafiltration step was subjected to a clarification step to remove most of the insoluble fibers followed by separation of patatin and protease inhibitors by ion exchange adsorption.

Ultrafiltration

[0302] The procedure was as follows: a 3 inch ROMICON PM30, 1.1 mm i.d. (Koch Membrane Systems, USA) hollow fiber membrane unit was employed. This unit has a membrane area of 2.3 m.sup.2. The cross flow was set at 34 L/min and the TMP (transmembrane pressure) was regulated to be approximately 1.2 bar.

[0303] The 30 L potato fruit juice was concentrated using ultrafiltration to a retentate volume of 6 L (i.e. the concentration factor was 5×) and the 24 L clear purple/brown permeate (labelled ‘first permeate’) was collected and stored at 2-4° C. until further processing. Using the built-in heat exchanger of the equipment, the temperature of the retentate (labelled ‘first retentate’) was kept in the range of 20-25° C. during the entire ultrafiltration procedure.

[0304] The flux observed in the beginning of the ultrafiltration process was approximately 35 LMH and thereafter gradually decreased to 15 LMH when the retentate was 5× concentrated. There were no signs of membrane fiber clogging due the high content of insoluble material.

Diafiltration

[0305] Following the 5×concentration, the 6 L, 3 g/L sodium sulfite was added to the first retentate (Sigma Aldrich USA, cat. No.: 13471), conductivity=4.1 mS/cm at 18° C., followed by further ultrafiltration to again reduce the retentate volume to 6 L while the corresponding permeate was collected in a separate vessel labelled ‘diafiltrate’. This procedure was repeated in total 6 times such that in total 36 L diafiltrate was obtained. The retentate was labelled ‘retentate’.

[0306] A sample of the ‘retentate’ obtained after diafiltration was diluted 20 times in 50 mM potassium phosphate at pH 7.0 and the O.D. 600 of the diluted sample was measured to be 0.6. Thus, the undiluted retentate had an O.D. 600 nm of 20×0.6=12.

[0307] The undiluted retentate had a pH value of 7.7 and a conductivity of 4.0 mS/cm. During diafiltration, a gradual increase of flux was observed and at the final stages the flux was in the range of 22 LMH when a 6 L diafiltration portion was added. This correlated well with a gradual increase of pH in the retentate for each diafiltration step added, which initially had a pH of 5.9 and at the end of this first diafiltration with sodium sulfite a pH of 7.7.

[0308] Analysis of the first 6×6 L diafiltration permeate for alkaline colored phenolic compounds showed that the concentration of phenolic compounds was very high in the first permeate fractions and then gradually decreased for each 6 L permeate fraction. The sixth permeate fraction was estimated to contain less than 10% phenolic compounds than the first permeate. Also, the majority of the total glycoalkaloids were analyzed to be in the first six permeate fractions, although still a significant concentration was also found in the subsequent fractions.

Clarification

[0309] The retentate was drained from the membrane filtration system (5.8 L retentate) and adjusted to pH 5.7 with sulfuric acid. The retentate was thereafter heated to 45° C. and centrifuged for 5 minutes at 4000 G.

[0310] A solid pellet was formed and the supernatant was a clear light brown liquid, indicating that practically all the insoluble fibers/material had been removed from the supernatant. The supernatant was decanted and used for the subsequent separation of patatin and protease inhibitors.

Separation of Patatin and Protease Inhibitors by Ion Exchange

[0311] The clarified retentate was mixed thoroughly in a stirred tank with a cation exchange adsorbent (SP Sepharose Fast Flow, Cytiva, USA) previously washed and equilibrated with water. Mixing was performed for one hour to ensure efficient binding of the protease inhibitors to the adsorbent. Following mixing, the suspension was poured onto a suction filter funnel to separate the adsorbent from the non-bound protein fraction. When the non-bound fraction was separated, the adsorbent was washed with water to wash out any further entrained non-bound fraction and the wash fraction was combined with the first separated non-bound fraction. The protein bound to the adsorbent was subsequently released and separated from the adsorbent by washing with 0.2 M sodium sulfate. Samples of the clarified retentate, the non-bound fraction, and the bound and subsequently released protein fraction were analyzed by SDS-PAGE. Results are depicted in FIG. 8 (lane 1: clarified retentate; lane: bound and subsequently released fraction; lane 3: non-bound fraction).

[0312] The SDS-PAGE analysis showed that the clarified retentate contains both patatin and protease inhibitors, while adsorption separates the two major protein groups to a large extent. The bound and subsequently released protein fraction contains almost exclusively the protease inhibitors and the non-bound fraction is highly enriched in patatin.

[0313] If need be, the two resulting protein fractions could be further purified to remove any remaining phenolic substances and/or glycoalkaloids and salts by ultrafiltration and diafiltration as described above.