Fungicidal composition having synergistic effect

Abstract

A fungicidal composition having a synergistic effect is provided. The composition including active ingredients A and B. The active ingredient A is benziothiazolinone, the active ingredient B is one selected from benthiavalicarb-isopropyl, zoxamide, prothioconazole, boscalid, fenamidone, fluopicolide, famoxadone, pyraclostrobin, picoxystrobin or fluazinam, and the weight ratio of the two ingredients is from 1:50 to 50:1. The test results show that the fungicidal composition according to the present invention has an obvious synergistic effect, such that the application rate is reduced and the cost is lowered; and is useful in controlling certain particular fungal diseases on grain crops, vegetables, and fruits with a broadened fungicidal spectrum, a retarded resistance development of the fungi, and an improved control effect.

Claims

1. A fungicidal composition having a synergistic effect, comprising active ingredients A and B, wherein: the active ingredient A is benziothiazolinone, the active ingredient B is one selected from the group consisting of benthiavalicarb-isopropyl, zoxamide, prothioconazole, boscalid, fluopicolide and fluazinam, and the weight ratio of the two ingredients is from 1:30 to 30:1.

2. The fungicidal composition according to claim 1, wherein the weight ratio of the active ingredient A to the active ingredient B is from 1:20 to 20:1.

3. The fungicidal composition according to claim 1, wherein the weight ratio of the active ingredient A to the active ingredient B is from 1:10 to 30:1.

4. The fungicidal composition according to claim 1, comprising 5-85% by weight of the active ingredients and 95-15% by weight of pesticide adjuvants.

5. The fungicidal composition according to claim 4, which is prepared into pesticidally acceptable formations with the active ingredients and the pesticide adjuvants.

6. The fungicidal composition according to claim 5, which is in the form of a wettable powder prepared with the active ingredients and the pesticide adjuvants.

7. The fungicidal composition according to claim 1 is used in the control of diseases on crops in the agricultural area.

8. The fungicidal composition according to claim 1 is used in the control of grape downy mildew, wheat rust, potato late blight, wheat powdery mildew, tomato grey mold or grape anthracnose.

9. The fungicidal composition according to claim 2 is used in the control of diseases on crops in the agricultural area.

10. The fungicidal composition according to claim 3 is used in the control of diseases on crops in the agricultural area.

11. The fungicidal composition according to claim 2 is used in the control of grape downy mildew, wheat rust, potato late blight, wheat powdery mildew, tomato grey mold or grape anthracnose.

12. The fungicidal composition according to claim 3 is used in the control of grape downy mildew, wheat rust, potato late blight, wheat powdery mildew, tomato grey mold or grape anthracnose.

13. The fungicidal composition according to claim 1, wherein the active ingredient B is selected from the group consisting of benthiavalicarb-isopropyl, zoxamide, and prothioconazole.

14. The fungicidal composition according to claim 1, wherein the active ingredient B is selected from the group consisting of boscalid, fluopicolide, and fluazinam.

15. The fungicidal composition according to claim 1, wherein the active ingredient B is fluopicolide.

16. The fungicidal composition according to claim 1, wherein the active ingredient B is selected from the group consisting of boscalid and fluazinam.

17. The fungicidal composition according to claim 1, wherein the active ingredient B is prothioconazole.

Description

DETAILED DESCRIPTION

(1) To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is described in further detail with reference to examples. It should be understood that specific examples described herein are merely provided for explaining, instead of limiting the present invention. Any modifications, equivalent replacements, and improvements made without departing from the spirit and principle of the present invention fall within the protection scope of the present invention.

(2) The percentages given in all the formulations in the examples below are all weight percentages. The various formulations are processed from the composition of the present invention by a process known in the prior art which may be varied as desired.

I. Preparation Example of Formulations

(3) The formulations processed from the fungicidal composition of the present invention are those known in the prior art. For intuitively and clearly show the synergistic effect between the active ingredients of the present invention, only the wettable powder is optionally prepared in the preparation examples of formulations and used as the pesticide formulation to validate the synergistic effect between the active ingredients.

(4) The active ingredients benziothiazolinone and one of benthiavalicarb-isopropyl, zoxamide, prothioconazole, boscalid, fenamidone, fluopicolide, famoxadone, pyraclostrobin, picoxystrobin or fluazinam were fully mixed with various adjuvants and fillers in proportion, and ground by an ultra-fine grinder, to obtain a wettable powder.

Example 1: 62% BenziothiazolinoneBenthiavalicarb-Isopropyl Wettable Powder

(5) benziothiazolinone 60%, benthiavalicarb-isopropyl 2%, a sodium alkyl naphthalene sulfonate 4%, sodium dodecyl sulfonate 3%, ammonium sulfate 3%, and light calcium carbonate q.s. to 100%.

Example 2: 50% BenziothiazolinoneBenthiavalicarb-Isopropyl Wettable Powder

(6) benziothiazolinone 25%, benthiavalicarb-isopropyl 25%, sodium lignin sulfonate 6%, sodium dodecyl sulfonate 3%, xanthan gum 1%, sodium carboxymethyl starch 1%, and attapulgite clay q.s. to 100%.

Example 3: 65% BenziothiazolinoneBenthiavalicarb-Isopropyl Wettable Powder

(7) benziothiazolinone 2%, benthiavalicarb-isopropyl 63%, sodium lignin sulfonate 5%, a sodium methylnaphthalene sulfonate formaldehyde condensate 7%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%

Example 4: 85% BenziothiazolinoneZoxamide Wettable Powder

(8) benziothiazolinone 83%, zoxamide 2%, ammonium sulfate 1%, sodium alginate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%

Example 5: 30% BenziothiazolinoneZoxamide Wettable Powder

(9) benziothiazolinone 15%, zoxamide 15%, sodium dodecyl sulfonate 2%, a sodium alkyl naphthalene sulfonate 2%, ammonium sulfate 3%, and light calcium carbonate q.s. to 100%.

Example 6: 75% BenziothiazolinoneZoxamide Wettable Powder

(10) benziothiazolinone 2%, zoxamide 73%, a sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignin sulfonate 4%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 7: 62% BenziothiazolinoneProthioconazole Wettable Powder

(11) benziothiazolinone 60%, prothioconazole 2%, sodium carboxymethyl starch 1%, sodium dodecyl sulfonate 4%, sodium lignin sulfonate 4%, xanthan gum 1%, and attapulgite clay q.s. to 100%.

Example 8: 40% BenziothiazolinoneProthioconazole Wettable Powder

(12) benziothiazolinone 20%, prothioconazole 20%, ammonium sulfate 1%, sodium alginate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%.

Example 9: 65% BenziothiazolinoneProthioconazole Wettable Powder

(13) benziothiazolinone 2%, prothioconazole 63%, a sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignin sulfonate 4%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 10: 65% BenziothiazolinoneBoscalid Wettable Powder

(14) benziothiazolinone 63%, boscalid 2%, calcium lignin sulfonate 2%, sodium dodecylbenzene sulfonate 1%, bentonite 2%, and attapulgite clay q.s. to 100%.

Example 11: 60% BenziothiazolinoneBoscalid Wettable Powder

(15) benziothiazolinone 30%, boscalid 30%, an alkylpolyoxyethylene ether sulfonate 1%, nekal 2%, bentonite 1.5%, white carbon black 2%, and diatomaceous earth q.s. to 100%.

Example 12: 62% BenziothiazolinoneBoscalid Wettable Powder

(16) benziothiazolinone 2%, boscalid 60%, a sodium alkylsulfonate 6%, sodium lignin sulfonate 6%, white carbon black 5%, and kaolin q.s. to 100%.

Example 13: 62% BenziothiazolinoneFenamidone Wettable Powder

(17) benziothiazolinone 60%, fenamidone 2%, a polyoxyethylene octyl phenyl ether 2%, sodium lignin sulfonate 6%, white carbon black 4%, and diatomaceous earth q.s. to 100%.

Example 14: 50% BenziothiazolinoneFenamidone Wettable Powder

(18) benziothiazolinone 25%, fenamidone 25%, calcium lignin sulfonate 7%, white carbon black 5%, sodium dodecylbenzene sulfonate 3%, and attapulgite clay q.s. to 100%.

Example 15: 62% BenziothiazolinoneFenamidone Wettable Powder

(19) benziothiazolinone 2%, fenamidone 60%, calcium lignin sulfonate 5%, bentonite 4%, a polyoxyethylene octyl phenyl ether 3%, and attapulgite clay q.s. to 100%.

Example 16: 65% BenziothiazolinoneFluopicolide Wettable Powder

(20) benziothiazolinone 63%, fluopicolide 2%, a polyoxyethylene octyl phenyl ether 1%, sodium lignin sulfonate 2%, white carbon black 3%, and diatomaceous earth q.s. to 100%.

Example 17: 50% BenziothiazolinoneFluopicolide Wettable Powder

(21) benziothiazolinone 25%, fluopicolide 25%, sodium dodecylbenzene sulfonate 3%, white carbon black 5%, calcium lignin sulfonate 7%, and attapulgite clay q.s. to 100%.

Example 18: 65% BenziothiazolinoneFluopicolide Wettable Powder

(22) benziothiazolinone 3%, fluopicolide 62%, calcium lignin sulfonate 5%, bentonite 4%, a polyoxyethylene octyl phenyl ether 3%, and attapulgite clay q.s. to 100%.

Example 19: 60% BenziothiazolinoneFamoxadone Wettable Powder

(23) benziothiazolinone 58%, famoxadone 2%, sodium dodecylbenzene sulfonate 2%, bentonite 1%, calcium lignin sulfonate 2%, and attapulgite clay q.s. to 100%.

Example 20: 50% BenziothiazolinoneFamoxadone Wettable Powder

(24) benziothiazolinone 25%, famoxadone 25%, sodium lignin sulfonate 6%, an alkylsulfonate 6%, white carbon black 11%, and kaolin q.s. to 100%.

Example 21: 60% BenziothiazolinoneFamoxadone Wettable Powder

(25) benziothiazolinone 2%, famoxadone 58%, nekal 1%, an alkylpolyoxyethylene ether sulfonate 2%, bentonite 1.5%, white carbon black 2%, and diatomaceous earth q.s. to 100%.

Example 22: 62% BenziothiazolinonePyraclostrobin Wettable Powder

(26) benziothiazolinone 60%, pyraclostrobin 2%, a sodium alkyl naphthalene sulfonate 4%, sodium dodecyl sulfonate 3%, ammonium sulfate 3%, and light calcium carbonate q.s. to 100%.

Example 23: 50% BenziothiazolinonePyraclostrobin Wettable Powder

(27) benziothiazolinone 25%, pyraclostrobin 25%, sodium lignin sulfonate 6%, sodium dodecyl sulfonate 3%, xanthan gum 1%, sodium carboxymethyl starch 1%, and attapulgite clay q.s. to 100%.

Example 24: 65% BenziothiazolinonePyraclostrobin Wettable Powder

(28) benziothiazolinone 2%, pyraclostrobin 63%, sodium lignin sulfonate 5%, a sodium methylnaphthalene sulfonate formaldehyde condensate 7%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 25: 65% BenziothiazolinonePicoxystrobin Wettable Powder

(29) benziothiazolinone 63%, picoxystrobin 2%, sodium alginate 3%, ammonium sulfate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%.

Example 26: 30% BenziothiazolinonePicoxystrobin Wettable Powder

(30) benziothiazolinone 15%, picoxystrobin 15%, sodium dodecyl sulfonate 2%, a sodium alkyl naphthalene sulfonate 2%, ammonium sulfate 3%, and light calcium carbonate q.s. to 100%.

Example 27: 75% BenziothiazolinonePicoxystrobin Wettable Powder

(31) benziothiazolinone 2%, picoxystrobin 73%, a sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignin sulfonate 4%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 28: 62% BenziothiazolinoneFluazinam Wettable Powder

(32) benziothiazolinone 60%, fluazinam 2%, ammonium sulfate 3%, sodium carboxymethyl starch 1%, sodium dodecyl sulfonate 4%, sodium lignin sulfonate 4%, xanthan gum 1%, and attapulgite clay q.s. to 100%.

Example 29: 40% BenziothiazolinoneFluazinam Wettable Powder

(33) benziothiazolinone 20%, fluazinam 20%, ammonium sulfate 1%, sodium alginate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%.

Example 30: 75% BenziothiazolinoneFluazinam Wettable Powder

(34) benziothiazolinone 2%, fluazinam 73%, a sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignin sulfonate 4%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

II. Efficacy Test

(I) Bioassay Examples

(35) 1. Toxicity test of benziothiazolinone combined respectively with benthiavalicarb-isopropyl, zoxamide, prothioconazole, boscalid, fenamidone, fluopicolide, famoxadone, pyraclostrobin, picoxystrobin, and fluazinam on downy mildew pathogens of grape

(36) Test target organisms: downy mildew pathogens of grape

(37) Based on the test grade scale, the disease development on the leaves of the whole grape plant was investigated, and the disease index and control effect were calculated.

(38) The control effect was converted into probability (y), the concentration of the agents (g/ml) in solution was converted into a logarithmic value (x), the toxic regression equation and the median inhibition concentration EC50 were calculated by least square method, and the toxicity index and the co-toxicity coefficient (CTC) of the agents were calculated by SUN Peiyun method.
Actual toxicity index (ATI)=(EC50 of standard/EC50 of test agent)*100
Theoretical toxicity index (TTI)=toxicity index of agent A*percentage content of A in the mixture+toxicity index of agent B*percentage content of B in the mixture
Co-toxicity coefficient (CTC)=[actual toxicity index (ATI) of the mixture/theoretical toxicity index (TTI) of the mixture]*100

(39) Where CTC80, the composition exhibits an antagonistic effect; where 80<CTC<120, the composition exhibits an additive effect, and where CTC120, the composition exhibits a synergistic effect.

(40) (1) Toxicity Test of Benziothiazolinone Combined with Benthiavalicarb-Isopropyl on Downy Mildew Pathogens of Grape

(41) TABLE-US-00001 TABLE 1 Toxicity test result analysis of benziothiazolinone combined with benthiavalicarb-isopropyl on downy mildew pathogens of grape Co-toxicity coefficient Name of agent EC.sub.50 (g/ml) ATI TTI (CTC) Benziothiazolinone 8.92 100 / / Benthiavalicarb-isopropyl 10.75 82.98 / / Benziothiazolinone:benthiavalicarb- 8.12 109.85 99.666 110.218 isopropyl = 50:1 Benziothiazolinone:benthiavalicarb- 7.28 122.53 99.451 123.206 isopropyl = 30:1 Benziothiazolinone:benthiavalicarb- 6.32 141.14 98.452 143.359 isopropyl = 10:1 Benziothiazolinone:benthiavalicarb- 5.83 153 91.488 167.235 isopropyl = 1:1 Benziothiazolinone:benthiavalicarb- 5.69 156.77 84.524 185.474 isopropyl = 1:10 Benziothiazolinone:benthiavalicarb- 8.48 105.19 83.526 125.937 isopropyl = 1:30 Benziothiazolinone:benthiavalicarb- 9.31 95.81 83.311 115.003 isopropyl = 1:50

(42) The results (in Table 1) show that the control effect of the combination of benziothiazolinone with benthiavalicarb-isopropyl on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(43) (2) Toxicity Test of Benziothiazolinone Combined with Zoxamide on Downy Mildew Pathogens of Grape

(44) TABLE-US-00002 TABLE 2 Toxicity test result analysis of benziothiazolinone combined with zoxamide on downy mildew pathogens of grape Co-toxicity coefficient Name of agent EC.sub.50 (g/ml) ATI TTI (CTC) Benziothiazolinone 9.41 100 / / Zoxamide 11.49 81.9 / / Benziothiazolinone:zoxamide = 50:1 9.16 102.73 99.645 103.096 Benziothiazolinone:zoxamide = 30:1 7.63 123.33 99.416 124.054 Benziothiazolinone:zoxamide = 10:1 6.51 144.55 98.354 146.969 Benziothiazolinone:zoxamide = 1:1 5.59 168.34 90.949 185.093 Benziothiazolinone:zoxamide = 1:10 5.93 158.68 83.543 189.938 Benziothiazolinone:zoxamide = 1:30 9.22 102.06 82.481 123.738 Benziothiazolinone:zoxamide = 1:50 10.15 92.71 82.252 112.715

(45) The results (in Table 2) show that the control effect of the combination of benziothiazolinone with zoxamide on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(46) (3) Toxicity Test of Benziothiazolinone Combined with Prothioconazole on Downy Mildew Pathogens of Grape

(47) TABLE-US-00003 TABLE 3 Toxicity test result analysis of benziothiazolinone combined with prothioconazole on downy mildew pathogens of grape Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 10.05 100 / / Prothioconazole 13.26 75.79 / / Benziothiazolinone:prothioconazole = 50:1 9.51 105.68 99.525 106.184 Benziothiazolinone:prothioconazole = 30:1 8.42 119.36 99.219 120.300 Benziothiazolinone:prothioconazole = 10:1 6.85 146.72 97.799 150.022 Benziothiazolinone:prothioconazole = 1:1 7.19 139.78 87.896 159.029 Benziothiazolinone:prothioconazole = 1:10 7.95 126.42 77.993 162.091 Benziothiazolinone:prothioconazole = 1:30 10.82 92.88 76.573 121.296 Benziothiazolinone:prothioconazole = 1:50 12.15 82.72 76.267 108.461

(48) The results (in Table 3) show that the control effect of the combination of benziothiazolinone with prothioconazole on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(49) (4) Toxicity Test of Benziothiazolinone Combined with Boscalid on Downy Mildew Pathogens of Grape

(50) TABLE-US-00004 TABLE 4 Toxicity test result analysis of benziothiazolinone combined with boscalid on downy mildew pathogens of grape Co-toxicity coefficient Name of agent EC.sub.50 (g/ml) ATI TTI (CTC) Benziothiazolinone 9.46 100 / / Boscalid 11.61 81.48 / / Benziothiazolinone:boscalid = 50:1 9.12 103.73 99.637 104.108 Benziothiazolinone:boscalid = 30:1 7.86 120.36 99.403 121.083 Benziothiazolinone:boscalid = 10:1 6.82 138.71 98.316 141.086 Benziothiazolinone:boscalid = 1:1 6.87 137.7 90.741 151.751 Benziothiazolinone:boscalid = 1:10 7.83 120.82 83.165 145.277 Benziothiazolinone:boscalid = 1:30 9.51 99.47 82.079 121.188 Benziothiazolinone:boscalid = 1:50 10.62 89.08 81.845 108.840

(51) The results (in Table 4) show that the control effect of the combination of benziothiazolinone with boscalid on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(52) (5) Toxicity Test of Benziothiazolinone Combined with Fenamidone on Downy Mildew Pathogens of Grape

(53) TABLE-US-00005 TABLE 5 Toxicity test result analysis of benziothiazolinone combined with fenamidone on downy mildew pathogens of grape Co-toxicity coefficient Name of agent EC.sub.50(g/ml) ATI TTI (CTC) Benziothiazolinone 10.59 100 / / Fenamidone 12.78 82.86 / / Benziothiazolinone:fenamidone = 10.13 104.54 99.664 104.892 50:1 Benziothiazolinone:fenamidone = 8.64 122.57 99.447 123.252 30:1 Benziothiazolinone:fenamidone = 6.68 158.53 98.442 161.039 10:1 Benziothiazolinone:fenamidone = 7.27 145.67 91.432 159.321 1:1 Benziothiazolinone:fenamidone = 7.92 133.71 84.422 158.383 1:10 Benziothiazolinone:fenamidone = 10.43 101.53 83.417 121.714 1:30 Benziothiazolinone:fenamidone = 12.27 86.31 83.2 103.738 1:50

(54) The results (in Table 5) show that the control effect of the combination of benziothiazolinone with fenamidone on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(55) (6) Toxicity Test of Benziothiazolinone Combined with Fluopicolide on Downy Mildew Pathogens of Grape

(56) TABLE-US-00006 TABLE 6 Toxicity test result analysis of benziothiazolinone combined with fluopicolide on downy mildew pathogens of grape Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 12.17 100 / / Fluopicolide 15.32 79.44 / / Benziothiazolinone:fluopicolide = 50:1 11.62 104.73 99.597 105.154 Benziothiazolinone:fluopicolide = 30:1 10.13 120.14 99.337 120.942 Benziothiazolinone:fluopicolide = 10:1 8.74 139.24 98.131 141.892 Benziothiazolinone:fluopicolide = 1:1 8.12 149.88 89.719 167.055 Benziothiazolinone:fluopicolide = 1:10 8.49 143.35 81.308 176.305 Benziothiazolinone:fluopicolide = 1:30 12.18 99.92 80.102 124.741 Benziothiazolinone:fluopicolide = 1:50 13.44 90.55 79.842 113.411

(57) The results (in Table 6) show that the control effect of the combination of benziothiazolinone with fluopicolide on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(58) (7) Toxicity Test of Benziothiazolinone Combined with Famoxadone on Downy Mildew Pathogens of Grape

(59) TABLE-US-00007 TABLE 7 Toxicity test result analysis of benziothiazolinone combined with famoxadone on downy mildew pathogens of grape Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 11.36 100 / / Famoxadone 13.81 82.26 / / Benziothiazolinone:famoxadone = 50:1 10.56 107.58 99.652 107.956 Benziothiazolinone:famoxadone = 30:1 9.47 119.96 99.428 120.650 Benziothiazolinone:famoxadone = 10:1 8.69 130.72 98.387 132.863 Benziothiazolinone:famoxadone = 1:1 8.21 138.37 91.13 151.838 Benziothiazolinone:famoxadone = 1:10 9.42 120.59 83.872 143.779 Benziothiazolinone:famoxadone = 1:30 11.08 102.53 82.832 123.781 Benziothiazolinone:famoxadone = 1:50 12.15 93.5 82.607 113.187

(60) The results (in Table 7) show that the control effect of the combination of benziothiazolinone with famoxadone on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(61) (8) Toxicity Test of Benziothiazolinone Combined with Pyraclostrobin on Downy Mildew Pathogens of Grape

(62) TABLE-US-00008 TABLE 8 Toxicity test result analysis of benziothiazolinone combined with pyraclostrobin on downy mildew pathogens of grape Co-toxicity coefficient Name of agent EC.sub.50 (g/ml) ATI TTI (CTC) Benziothiazolinone 11.85 100 / / pyraclostrobin 10.69 110.85 / / Benziothiazolinone:pyraclostrobin = 10.19 116.29 100.213 116.043 50:1 Benziothiazolinone:pyraclostrobin = 9.52 124.47 100.35 124.036 30:1 Benziothiazolinone:pyraclostrobin = 7.41 159.92 100.986 158.359 10:1 Benziothiazolinone:pyraclostrobin = 7.12 166.43 105.426 157.864 1:1 Benziothiazolinone:pyraclostrobin = 8.27 143.29 109.865 130.424 1:10 Benziothiazolinone:pyraclostrobin = 8.71 136.05 110.501 123.121 1:30 Benziothiazolinone:pyraclostrobin = 10.15 116.75 110.638 105.524 1:50

(63) The results (in Table 8) show that the control effect of the combination of benziothiazolinone with pyraclostrobin on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(64) (9) Toxicity Test of Benziothiazolinone Combined with Picoxystrobin on Downy Mildew Pathogens of Grape

(65) TABLE-US-00009 TABLE 9 Toxicity test result analysis of benziothiazolinone combined with picoxystrobin on downy mildew pathogens of grape Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 14.18 100 / / Picoxystrobin 15.31 92.62 / / Benziothiazolinone:picoxystrobin = 50:1 13.08 108.41 99.855 108.567 Benziothiazolinone:picoxystrobin = 30:1 11.46 123.73 99.762 124.025 Benziothiazolinone:picoxystrobin = 10:1 9.87 143.67 99.329 144.641 Benziothiazolinone:picoxystrobin = 1:1 9.93 142.8 96.31 148.271 Benziothiazolinone:picoxystrobin = 1:10 10.16 139.57 93.29 149.609 Benziothiazolinone:picoxystrobin = 1:30 12.42 114.17 92.857 122.952 Benziothiazolinone:picoxystrobin = 1:50 14.45 98.13 92.764 105.785

(66) The results (in Table 9) show that the control effect of the combination of benziothiazolinone with picoxystrobin on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(67) (10) Toxicity Test of Benziothiazolinone Combined with Fluazinam on Downy Mildew Pathogens of Grape

(68) TABLE-US-00010 TABLE 10 Toxicity test result analysis of benziothiazolinone combined with fluazinam on downy mildew pathogens of grape Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone hydrochloride 12.87 100 / / Fluazinam 11.24 114.5 / / Benziothiazolinone:fluazinam = 50:1 11.16 115.32 100.284 114.993 Benziothiazolinone:fluazinam = 30:1 10.22 125.93 100.468 125.343 Benziothiazolinone:fluazinam = 10:1 8.27 155.62 101.318 153.596 Benziothiazolinone:fluazinam = 1:1 7.81 164.79 107.251 153.649 Benziothiazolinone:fluazinam = 1:10 8.12 158.5 113.183 140.039 Benziothiazolinone:fluazinam = 1:30 9.18 140.2 114.034 122.946 Benziothiazolinone:fluazinam = 1:50 10.45 123.16 114.217 107.830

(69) The results (in Table 10) show that the control effect of the combination of benziothiazolinone with fluazinam on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of grape.

(70) 2. Toxicity Test of Benziothiazolinone Combined Respectively with Pyraclostrobin, Boscalid, Benthiavalicarb-Isopropyl, Zoxamide, and Fenamidone on Late Blight Pathogens of Potato

(71) The test method was the same as above. (The test results are shown in Tables 11, 12, 13, 14, and 15 respectively)

(72) (1) Toxicity Test of Benziothiazolinone Combined with Pyraclostrobin on Late Blight Pathogens of Potato

(73) TABLE-US-00011 TABLE 11 Toxicity test result analysis of benziothiazolinone combined with pyraclostrobin on late blight pathogens of potato Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 6.89 100.00 / / pyraclostrobin 5.28 130.49 / / Benziothiazolinone:pyraclostrobin = 50:1 5.72 120.45 100.598 119.74 Benziothiazolinone:pyraclostrobin = 30:1 5.44 126.65 100.984 125.42 Benziothiazolinone:pyraclostrobin = 10:1 4.63 148.81 102.772 144.80 Benziothiazolinone:pyraclostrobin = 1:1 3.32 207.53 115.246 180.08 Benziothiazolinone:pyraclostrobin = 1:10 2.93 235.15 127.720 184.12 Benziothiazolinone:pyraclostrobin = 1:30 4.21 163.66 129.509 126.37 Benziothiazolinone:pyraclostrobin = 1:50 4.68 147.22 129.895 113.34

(74) The results (in Table 11) show that the control effect of the combination of benziothiazolinone with pyraclostrobin on late blight of potato is significantly improved, suggesting that the combination has an obvious synergistic effect on late blight pathogens of potato.

(75) (2) Toxicity Test of Benziothiazolinone Combined with Boscalid on Late Blight Pathogens of Potato

(76) TABLE-US-00012 TABLE 12 Toxicity test result analysis of benziothiazolinone combined with boscalid on late blight pathogens of potato Co-toxicity coefficient Name of agent EC.sub.50 (g/ml) ATI TTI (CTC) Benziothiazolinone 5.58 100.00 / / Boscalid 6.31 88.43 / / Benziothiazolinone:boscalid = 50:1 4.78 116.74 99.7732 117.005 Benziothiazolinone:boscalid = 30:1 4.62 120.78 99.6268 121.232 Benziothiazolinone:boscalid = 10:1 4.21 132.54 98.9483 133.950 Benziothiazolinone:boscalid = 1:1 3.28 170.12 94.2155 180.567 Benziothiazolinone:boscalid = 1:10 3.18 175.47 89.4828 196.095 Benziothiazolinone:boscalid = 1:30 4.91 113.65 88.8043 127.973 Benziothiazolinone:boscalid = 1:50 5.55 100.54 88.6579 113.403

(77) The results (in Table 12) show that the control effect of the combination of benziothiazolinone with boscalid on late blight of potato is significantly improved, suggesting that the combination has an obvious synergistic effect on late blight pathogens of potato.

(78) (3) Toxicity Test of Benziothiazolinone Combined with Benthiavalicarb-Isopropyl on Late Blight Pathogens of Potato

(79) TABLE-US-00013 TABLE 13 Toxicity test result analysis of benziothiazolinone combined with benthiavalicarb-isopropyl on late blight pathogens of potato Co-toxicity coefficient Name of agent EC.sub.50 (g/ml) ATI TTI (CTC) Benziothiazolinone 6.22 100.000 / / Benthiavalicarb-isopropyl 6.73 92.422 / / Benziothiazolinone:benthiavalicarb- 5.43 114.549 99.851 114.719 isopropyl = 50:1 Benziothiazolinone:benthiavalicarb- 5.11 121.722 99.756 122.020 isopropyl = 30:1 Benziothiazolinone:benthiavalicarb- 4.17 149.161 99.311 150.195 isopropyl = 10:1 Benziothiazolinone:benthiavalicarb- 3.69 168.564 96.211 175.202 isopropyl = 1:1 Benziothiazolinone:benthiavalicarb- 4.25 146.353 93.111 157.181 isopropyl = 1:10 Benziothiazolinone:benthiavalicarb- 5.23 118.929 92.666 128.341 isopropyl = 1:30 Benziothiazolinone:benthiavalicarb- 5.61 110.873 92.571 119.772 isopropyl = 1:50

(80) The results (in Table 13) show that the control effect of the combination of benziothiazolinone with benthiavalicarb-isopropyl on late blight of potato is significantly improved, suggesting that the combination has an obvious synergistic effect on late blight pathogens of potato.

(81) (4) Toxicity Test of Benziothiazolinone Combined with Zoxamide on Late Blight Pathogens of Potato

(82) TABLE-US-00014 TABLE 14 Toxicity test result analysis of benziothiazolinone combined with zoxamide on late blight pathogens of potato Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 5.89 100.00 / / Zoxamide 6.21 94.85 / / Benziothiazolinone:zoxamide = 50:1 5.21 113.05 99.899 113.164 Benziothiazolinone:zoxamide = 30:1 4.82 122.2 99.834 122.403 Benziothiazolinone:zoxamide = 10:1 4.09 144.01 99.532 144.687 Benziothiazolinone:zoxamide = 1:1 3.58 164.53 97.424 168.880 Benziothiazolinone:zoxamide = 1:10 4.05 145.43 95.315 152.578 Benziothiazolinone:zoxamide = 1:30 5.21 113.05 95.013 118.984 Benziothiazolinone:zoxamide = 1:50 5.42 108.67 94.948 114.452

(83) The results (in Table 14) show that the control effect of the combination of benziothiazolinone with zoxamide on late blight of potato is significantly improved, suggesting that the combination has an obvious synergistic effect on late blight pathogens of potato.

(84) (5) Toxicity Test of Benziothiazolinone Combined with Fenamidone on Late Blight Pathogens of Potato

(85) TABLE-US-00015 TABLE 15 Toxicity test result analysis of benziothiazolinone combined with fenamidone on late blight pathogens of potato Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 6.12 100.00 / / Fenamidone 6.58 93.01 / / Benziothiazolinone:fenamidone = 50:1 5.31 115.25 99.863 115.408 Benziothiazolinone:fenamidone = 30:1 4.52 135.4 99.774 135.707 Benziothiazolinone:fenamidone = 10:1 4.15 147.47 99.364 148.414 Benziothiazolinone:fenamidone = 1:1 3.61 169.53 96.505 175.670 Benziothiazolinone:fenamidone = 1:10 4.23 144.68 93.645 154.498 Benziothiazolinone:fenamidone = 1:30 5.16 118.6 93.235 127.205 Benziothiazolinone:fenamidone = 1:50 5.37 113.97 93.146 122.356

(86) The results (in Table 15) show that the control effect of the combination of benziothiazolinone with fenamidone on late blight of potato is significantly improved, suggesting that the combination has an obvious synergistic effect on late blight pathogens of potato.

(87) 3. Toxicity Test of Benziothiazolinone Combined Respectively with Fluazinam, Prothioconazole, Picoxystrobin, Fluopicolide, and Famoxadone on Rust Pathogens of Wheat

(88) Test target organism: rust pathogens of wheat

(89) The test method was the same as above. (The test results are shown in Tables 16, 17, 18, 19, and 20 respectively)

(90) (1) Toxicity Test of Benziothiazolinone Combined with Fluazinam on Rust Pathogens of Wheat

(91) TABLE-US-00016 TABLE 16 Toxicity test result analysis of benziothiazolinone combined with fluazinam on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone hydrochloride 7.68 100 / / Fluazinam 8.15 94.23 / / Benziothiazolinone:fluazinam = 50:1 6.82 112.61 99.887 112.737 Benziothiazolinone:fluazinam = 30:1 6.41 119.81 99.814 120.033 Benziothiazolinone:fluazinam = 10:1 5.68 135.21 99.476 135.922 Benziothiazolinone:fluazinam = 1:1 5.21 147.41 97.117 151.786 Benziothiazolinone:fluazinam = 1:10 5.56 138.13 94.757 145.773 Benziothiazolinone:fluazinam = 1:30 6.46 118.89 94.419 125.917 Benziothiazolinone:fluazinam = 1:50 6.87 111.79 94.346 118.489

(92) The results (in Table 16) show that the control effect of the combination of benziothiazolinone with fluazinam on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat.

(93) (2) Toxicity Test of Benziothiazolinone Combined with Prothioconazole on Rust Pathogens of Wheat

(94) TABLE-US-00017 TABLE 17 Toxicity test result analysis of benziothiazolinone combined with prothioconazole on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 7.92 100 / / Prothioconazole 7.68 103.13 / / Benziothiazolinone:prothioconazole = 50:1 7.11 111.39 100.061 111.322 Benziothiazolinone:prothioconazole = 30:1 6.45 122.79 100.101 122.666 Benziothiazolinone:prothioconazole = 10:1 5.61 141.18 100.284 140.780 Benziothiazolinone:prothioconazole = 1:1 4.63 171.06 101.563 168.427 Benziothiazolinone:prothioconazole = 1:10 5.27 150.28 102.841 146.128 Benziothiazolinone:prothioconazole = 1:30 6.21 127.54 103.024 123.796 Benziothiazolinone:prothioconazole = 1:50 6.62 119.64 103.064 116.083

(95) The results (in Table 17) show that the control effect of the combination of benziothiazolinone with prothioconazole on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat.

(96) (3) Toxicity Test of Benziothiazolinone Combined with Picoxystrobin on Rust Pathogens of Wheat

(97) TABLE-US-00018 TABLE 18 Toxicity test result analysis of benziothiazolinone combined with picoxystrobin on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) benziothiazolinone 7.51 100 / / picoxystrobin 8.26 90.92 / / benziothiazolinone:picoxystrobin = 50:1 6.52 115.18 99.822 115.385 benziothiazolinone:picoxystrobin = 30:1 6.21 120.93 99.707 121.285 benziothiazolinone:picoxystrobin = 10:1 5.48 137.04 99.175 138.180 benziothiazolinone:picoxystrobin = 1:1 4.31 174.25 95.46 182.537 benziothiazolinone:picoxystrobin = 1:10 5.18 144.98 91.746 158.023 benziothiazolinone:picoxystrobin = 1:30 6.72 111.76 91.213 122.526 benziothiazolinone:picoxystrobin = 1:50 6.98 107.59 91.098 118.104

(98) The results (in Table 18) show that the control effect of the combination of benziothiazolinone with picoxystrobin on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat.

(99) (4) Toxicity Test of Benziothiazolinone Combined with Fluopicolide on Rust Pathogens of Wheat

(100) TABLE-US-00019 TABLE 19 Toxicity test result analysis of benziothiazolinone combined with fluopicolide on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 7.93 100 / / Fluopicolide 7.69 103.12 / / Benziothiazolinone:fluopicolide = 50:1 6.98 113.61 100.061 113.541 Benziothiazolinone:fluopicolide = 30:1 6.32 125.47 100.101 125.343 Benziothiazolinone:fluopicolide = 10:1 5.26 150.76 100.284 150.333 Benziothiazolinone:fluopicolide = 1:1 4.91 161.51 101.56 159.029 Benziothiazolinone:fluopicolide = 1:10 5.32 149.06 102.837 144.948 Benziothiazolinone:fluopicolide = 1:30 6.66 119.07 103.02 115.579 Benziothiazolinone:fluopicolide = 1:50 6.85 115.77 103.06 112.333

(101) The results (in Table 19) show that the control effect of the combination of benziothiazolinone with fluopicolide on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat.

(102) (5) Toxicity Test of Benziothiazolinone Combined with Famoxadone on Rust Pathogens of Wheat

(103) TABLE-US-00020 TABLE 20 Toxicity test result analysis of benziothiazolinone combined with famoxadone on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Benziothiazolinone 7.51 100 / / Famoxadone 7.96 94.35 / / Benziothiazolinone:famoxadone = 50:1 6.51 115.36 99.889 115.488 Benziothiazolinone:famoxadone = 30:1 6.17 121.72 99.818 121.942 Benziothiazolinone:famoxadone = 10:1 5.12 146.68 99.486 147.438 Benziothiazolinone:famoxadone = 1:1 4.56 164.69 97.173 169.481 Benziothiazolinone:famoxadone = 1:10 5.27 142.5 94.861 150.220 Benziothiazolinone:famoxadone = 1:30 6.52 115.18 94.529 121.846 Benziothiazolinone:famoxadone = 1:50 7.21 104.16 94.458 110.271

(104) The results (in Table 20) show that the control effect of the combination of benziothiazolinone with famoxadone on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat.

(II) Field Efficacy Test

(105) Test method: in early stage of disease development, the first spray was given immediately, and then the second application was given after 7 days. Each treatment included 4 plots of 20 square meters each. The disease development before application and 10 days after the second application was statistically investigated. Samples were collected from 5 locations in each plot at random, and 5 plants were investigated at each location by investigating the percentages of the disease spot area on the leaves relative to the leaf area of the whole plant and grading. The disease index and the control effect were calculated.

(106) $\mathrm{Disease}\mathrm{index}=\frac{.Math.\left(\begin{array}{c}\begin{array}{c}\mathrm{Number}\mathrm{of}\mathrm{leaves}\mathrm{at}\mathrm{each}\\ \mathrm{grade}\mathrm{of}\mathrm{disease}\mathrm{development}\end{array}\\ \begin{array}{c}\mathrm{Representative}\mathrm{value}\\ \mathrm{of}\mathrm{corresponding}\mathrm{grade}\end{array}\end{array}\right)}{\left(\begin{array}{c}\mathrm{Total}\mathrm{number}\mathrm{of}\\ \mathrm{leaves}\mathrm{investigated}\end{array}\begin{array}{c}\mathrm{Representative}\\ \mathrm{value}\mathrm{of}\mathrm{highest}\mathrm{level}\end{array}\right)}100$$\mathrm{Control}\mathrm{effect}\left(\%\right)=\left(1-\frac{\begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{control}\\ \mathrm{group}\mathrm{before}\mathrm{application}\end{array}\begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{treatment}\\ \mathrm{group}\mathrm{after}\mathrm{application}\end{array}}{\begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{control}\\ \mathrm{group}\mathrm{after}\mathrm{application}\end{array}\begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{treatment}\\ \mathrm{group}\mathrm{before}\mathrm{application}\end{array}}\right)100$
Anticipated control effect (%)=X+YXY/100 (where X and Y are the control effect of a single agent)

(107) Grade Scale:

(108) Grade 0: no disease spot;

(109) Grade 1: number of disease spots on the leaf<5, and length<1 cm;

(110) Grade 3: 6number of disease spots on the leaf10, and length of some disease spots>1 cm;

(111) Grade 5: 11number of disease spots on the leaf25, some disease spots are contiguous, and the disease spot area is 10-25% of the leaf area;

(112) Grade 7: number of disease spots on the leaf26, the disease spots are contiguous, and the disease spot area is 26-50% of the leaf area;

(113) Grade 9: the disease spots are contiguous, and the disease spot area is above 50% of the leaf area, or all the leaves all wilted.

(114) 1. Field Efficacy Test of Benziothiazolinone Combined Respectively with Pyraclostrobin, Prothioconazole, Picoxystrobin, Fluopicolide, and Famoxadone for Controlling Wheat Powdery Mildew

(115) TABLE-US-00021 TABLE 21 Field efficacy test of benziothiazolinone combined with the above fungicides for wheat powdery mildew Day 11 after the second Disease application Application index Control rate before Disease effect No. Treatment agent (a.i.g/ha) application index (%) Example 5% benziothiazolinone 145.2 4.02 7.51 75.2 22 microemulsion 10% pyraclostrobin aqueous 4.8 4.14 29.24 6.2 solution Anticipated control effect after 76.7 mixing them 62% 150 4.43 5.40 83.8 benziothiazolinonepyraclostrobin wettable powder (benziothiazolinone:pyraclostrobin = 60:2) Example 5% benziothiazolinone 75 4.72 20.19 43.2 23 microemulsion 10% pyraclostrobin aqueous 75 4.83 19.60 46.1 solution Anticipated control effect after 69.4 mixing them 50% 150 4.19 5.43 82.8 benziothiazolinonepyraclostrobin wettable powder (benziothiazolinone:pyraclostrobin = 25:25) Example 5% benziothiazolinone 4.8 4.6 33.22 4.1 24 microemulsion 10% pyraclostrobin aqueous 145.2 4.63 8.05 76.9 solution Anticipated control effect after 77.8 mixing them 65% 150 4.62 5.18 85.1 benziothiazolinonepyraclostrobin wettable powder (benziothiazolinone:pyraclostrobin = 2:63) Example 7 5% benziothiazolinone 145.2 4.46 9.10 72.9 microemulsion 25% prothioconazole wettable 4.8 4.79 34.20 5.2 powder Anticipated control effect after 74.3 mixing them 62% 150 4.74 5.07 85.8 benziothiazolinoneprothioconazole wettable powder (benziothiazolinone:prothioconazole = 60:2) Example 8 5% benziothiazolinone 75 4.47 19.02 43.5 microemulsion 25% prothioconazole wettable 75 4.25 17.22 46.2 powder Anticipated control effect after 69.6 mixing them 40% 150 4.36 5.29 83.9 benziothiazolinoneprothioconazole wettable powder (benziothiazolinone:prothioconazole = 20:20) Example 9 5% benziothiazolinone 4.6 4.63 33.51 3.9 microemulsion 25% prothioconazole wettable 145.4 4.04 6.75 77.8 powder Anticipated control effect after 78.7 mixing them 65% 150 4.33 4.83 85.2 benziothiazolinoneprothioconazole wettable powder (benziothiazolinone:prothioconazole = 2:63) Example 5% benziothiazolinone 145.4 4.17 7.66 75.6 25 microemulsion 15% picoxystrobin concentrate 4.6 4.02 28.79 4.9 Anticipated control effect after 76.8 mixing them 65% 150 4.23 4.84 84.8 benziothiazolinonepicoxystrobin wettable powder (benziothiazolinone:picoxystrobin = 63:2) Example 5% benziothiazolinone 75 4.47 18.98 43.6 26 microemulsion 15% picoxystrobin concentrate 75 4.16 17.17 45.2 Anticipated control effect after 69.1 mixing them 30% 150 4.32 4.52 86.1 benziothiazolinonepicoxystrobin wettable powder (benziothiazolinone:picoxystrobin = 15:15) Example 5% benziothiazolinone 4 4.42 31.99 3.9 27 microemulsion 15% picoxystrobin concentrate 146 4.03 6.92 77.2 Anticipated control effect after 78.1 mixing them 75% 150 4.02 4.21 86.1 benziothiazolinonepicoxystrobin wettable powder (benziothiazolinone:picoxystrobin = 2:73) Example 5% benziothiazolinone 146 4.13 7.43 76.1 16 microemulsion 10% fluopicolide suspension 4 3.87 27.63 5.2 Anticipated control effect after 77.3 mixing them 65% 150 3.81 4.33 84.9 benziothiazolinonefluopicolide wettable powder (benziothiazolinone:picoxystrobin = 63:2) Example 5% benziothiazolinone 75 3.77 16.07 43.4 17 microemulsion 10% fluopicolide suspension 75 3.82 15.48 46.2 Anticipated control effect after 69.5 mixing them 50% 150 4.1 3.98 87.1 benziothiazolinonefluopicolide wettable powder (benziothiazolinone:fluopicolide = 25:25) Example 5% benziothiazolinone 4 4.23 30.58 4.0 18 microemulsion 10% fluopicolide suspension 146 4.02 7.54 75.1 Anticipated control effect after 76.1 mixing them 65% 150 4.43 5.07 84.8 benziothiazolinonefluopicolide wettable powder (benziothiazolinonefluopicolide = 2:63) Example 5% benziothiazolinone 145 4.23 7.74 75.7 19 microemulsion 30% famoxadone wettable 5 4.8 33.91 6.2 powder Anticipated control effect after 77.2 mixing them 60% 150 4.42 4.63 86.1 benziothiazolinonefamoxadone wettable powder (benziothiazolinone:famoxadone = 58:2) Example 5% benziothiazolinone 75 3.76 16.11 43.1 20 microemulsion 30% famoxadone wettable 75 3.89 15.76 46.2 powder Anticipated control effect after 69.4 mixing them 50% 150 4.06 4.34 85.8 benziothiazolinonefamoxadone wettable powder (benziothiazolinone:famoxadone = 25:25) Example 5% benziothiazolinone 5 4.12 29.57 4.7 21 microemulsion 30% famoxadone wettable 150 4.33 8.09 75.2 powder Anticipated control effect after 76.4 mixing them 60% 150 4.3 4.50 86.1 benziothiazolinonefamoxadone wettable powder (benziothiazolinone:famoxadone = 2:58) Water 3.28 24.7 control (CK)

(116) The test results (in Table 21) show that the control effect of the combination of benziothiazolinone with pyraclostrobin, prothioconazole, picoxystrobin, fluopicolide, and famoxadone respectively on wheat powdery mildew is significantly improved, suggesting that the combination has an obvious synergistic effect on wheat powdery mildew.

(117) (2) Field Efficacy Test of Benziothiazolinone Combined Respectively with Fluazinam, Boscalid, and Fluopicolide for Controlling Tomato Grey Mold

(118) TABLE-US-00022 TABLE 22 Field efficacy test of benziothiazolinone or a salt thereof combined respectively with the above fungicides for tomato grey mold Day 11 after the second Disease application Application index Control rate before Disease effect No. Treatment agent (a.i.g/ha) application index (%) Example 5% benziothiazolinone 117 5.9 3.03 82.15 28 microemulsion 12.5% fluazinam suspension 3 6.23 17.42 2.95 Anticipated control effect after 82.68 mixing them 62% benziothiazolinonefluazinam 120 6.03 2.44 85.95 wettable powder (benziothiazolinone:fluazinam = 60:2) Example 5% benziothiazolinone 60 6.12 8.05 54.35 29 microemulsion 12.5% fluazinam suspension 60 6.23 10.42 41.95 Anticipated control effect after 73.50 mixing them 40% benziothiazolinonefluazinam 120 6.25 2.28 87.35 wettable powder (benziothiazolinone:fluazinam = 20:20) Example 5% benziothiazolinone 3.2 5.83 15.77 6.15 30 microemulsion 12.5% fluazinam suspension 116.8 5.78 4.12 75.25 Anticipated control effect after 76.77 mixing them 75% benziothiazolinone 120 5.89 2.49 85.35 carbonatefluazinam wettable powder (benziothiazolinone:fluazinam = 2:73) Example 5% benziothiazolinone 116.3 5.67 2.85 82.55 10 microemulsion 20% boscalid aqueous emulsion 3.7 5.83 16.25 3.25 Anticipated control effect after 83.12 mixing them 65% benziothiazolinoneboscalid 120 5.7 2.24 86.35 wettable powder (benziothiazolinone:boscalid = 63:2) Example 5% benziothiazolinone 60 5.89 7.76 54.25 11 microemulsion 20% boscalid aqueous emulsion 60 6.23 9.43 47.45 Anticipated control effect after 75.96 mixing them 60% benziothiazolinoneboscalid 120 5.9 2.17 87.25 wettable powder (benziothiazolinone:boscalid = 30:30) Example 5% benziothiazolinone 3.9 6.12 16.73 5.15 12 microemulsion 20% boscalid aqueous emulsion 116.1 5.78 4.12 75.25 Anticipated control effect after 76.52 mixing them 62% benziothiazolinoneboscalid 120 6.03 2.46 85.85 wettable powder (benziothiazolinone:boscalid = 2:60) Example 5% benziothiazolinone 117.3 6.33 2.96 83.75 16 microemulsion 30% fluopicolide aqueous emulsion 2.8 6.33 17.83 2.25 Anticipated control effect after 84.12 mixing them 65% benziothiazolinonefluopicolide 120 6.23 2.49 86.15 wettable powder (benziothiazolinone:fluopicolide = 63:2) Example 5% benziothiazolinone 60 6.53 8.42 55.25 17 microemulsion 30% fluopicolide aqueous emulsion 60 6.14 9.05 48.85 Anticipated control effect after 77.11 mixing them 50% benziothiazolinonefluopicolide 120 6.16 2.60 85.35 wettable powder (benziothiazolinone:fluopicolide = 25:25) Example 5% benziothiazolinone 3.7 5.88 16.00 5.55 18 microemulsion 30% fluopicolide aqueous emulsion 116.3 5.9 2.95 82.65 Anticipated control effect after 83.61 mixing them 65% benziothiazolinonefluopicolide 120 6.06 2.38 86.35 wettable powder (benziothiazolinone:fluopicolide = 2:63) Water Water control 6.08 17.52 control (CK)

(119) The test results (in Table 22) show that the control effect of the combination of benziothiazolinone with fluazinam, boscalid, and fluopicolide respectively on tomato grey mold is significantly improved, suggesting that the combination has an obvious synergistic effect on tomato grey mold.

(120) (3) Field Efficacy Test of Benziothiazolinone Combined Respectively with Benthiavalicarb-Isopropyl, Zoxamide, and Fenamidone for Controlling Grape Anthracnose

(121) TABLE-US-00023 TABLE 23 Field efficacy test of benziothiazolinone combined respectively with the above fungicides for grape anthracnose Day 11 after the second application Application Disease index Control rate before Disease effect No. Treatment agent (a.i.g/ha) application index (%) Example 1 5% benziothiazolinone 130.2 3.89 4.65 83.1 microemulsion 10% benthiavalicarb-isopropyl 4.8 3.98 26.83 4.6 wettable powder Anticipated control effect after 83.9 mixing them 62% 135 3.87 3.77 86.2 benziothiazolinonebenthiavalicarb- isopropyl wettable powder (benziothiazolinone:benthiavalicarb- isopropyl = 60:2) Example 2 5% benziothiazolinone 67.5 3.76 14.06 47.1 microemulsion 10% benthiavalicarb-isopropyl 67.5 4.04 16.22 43.2 wettable powder Anticipated control effect after 70.0 mixing them 50% 135 3.93 3.58 87.1 benziothiazolinonebenthiavalicarb- isopropyl wettable powder (benziothiazolinone:benthiavalicarb- isopropyl = 25:25) Example 3 5% benziothiazolinone 3.6 3.87 25.38 7.2 microemulsion 10% benthiavalicarb-isopropyl 131.4 3.87 5.96 78.2 wettable powder Anticipated control effect after 79.8 mixing them 65% 135 3.94 3.87 86.1 benziothiazolinonebenthiayalicarb- isopropyl wettable powder (benziothiazolinone:benthiayalicarb- isopropyl = 2:63) Example 4 5% benziothiazolinone 130.6 3.65 4.62 82.1 microemulsion 15% zoxamide wettable powder 4.4 3.89 26.20 4.7 Anticipated control effect after 82.9 mixing them 85% benziothiazolinonezoxamide 135 3.79 3.72 86.1 wettable powder (benziothiazolinone:zoxamide = 83:2) Example 5 5% benziothiazolinone 67.5 3.78 14.00 47.6 microemulsion 15% zoxamide wettable powder 67.5 3.85 15.10 44.5 Anticipated control effect after 70.9 mixing them 30% benziothiazolinonezoxamide 135 3.91 3.81 86.2 wettable powder (benziothiazolinone:zoxamide = 15:15) Example 6 5% benziothiazolinone 4.2 3.86 25.12 7.9 microemulsion 15% zoxamide wettable powder 130.8 3.82 5.37 80.1 Anticipated control effect after 81.7 mixing them 75% benziothiazolinonezoxamide 135 3.79 3.96 85.2 wettable powder benziothiazolinone:zoxamide = 2:73) Example 5% benziothiazolinone 131.8 3.91 5.44 80.3 13 microemulsion 20% fenamidone wettable powder 3.2 3.21 21.57 4.9 Anticipated control effect after 81.3 mixing them 62% 135 3.79 3.80 85.8 benziothiazolinonefenamidone wettable powder (benziothiazolinone:fenamidone = 60:2) Example 5% benziothiazolinone 67.5 3.98 14.60 48.1 14 microemulsion 20% fenamidone wettable powder 67.5 4.05 15.31 46.5 Anticipated control effect after 72.2 mixing them 50% 135 3.99 3.33 88.2 benziothiazolinonefenamidone wettable powder (benziothiazolinone:fenamidone = 25:25) Example 5% benziothiazolinone 3.5 3.73 24.49 7.1 15 microemulsion 20% fenamidone wettable powder 131.5 3.32 4.62 80.3 Anticipated control effect after 81.7 mixing them 62% 135 3.54 3.38 86.5 benziothiazolinonefenamidone wettable powder (benziothiazolinone:fenamidone = 2:60) Water 3.87 27.35 control (CK)

(122) The test results (in Table 23) show that the control effect of the combination of benziothiazolinone with benthiavalicarb-isopropyl, zoxamide, and fenamidone respectively on grape anthracnose is significantly improved, suggesting that the combination has an obvious synergistic effect on grape anthracnose.