A COMPOSITION WITH MOBILITY BENEFITS IN AGEING, HEALTHY SUBJECTS

Abstract

The invention provides a composition useful in maintaining or improving mobility, preferably joint function and muscle strength in ageing, healthy subjects. The composition includes specific amounts of glucosamine or a pharmaceutically-acceptable derivative thereof, calcium, vitamin C, vitamin D and zinc. Also provided is the composition in the form of a powdered food product, and a beverage product comprising the composition in the form of a powder which has been reconstituted in a liquid. Also provided is the use of the composition or food or beverage product in maintaining or improving mobility, preferably joint function and muscle strength in ageing, healthy subjects.

Claims

1. A composition comprising: about 1500 mg to about 9000 mg of glucosamine or a pharmaceutically-acceptable derivative thereof per 100 g of the composition; about 300 mg to about 2000 mg of calcium per 100 g of the composition; about 50 mg to about 150 mg of vitamin C per 100 g of the composition; about 3 g to about 30 g of vitamin D per 100 g of the composition; and about 5 mg to about 25 mg of zinc per 100 g of the composition.

2. The composition of claim 1, wherein the composition comprises: about 2500 mg to about 8000 mg of glucosamine or a pharmaceutically-acceptable derivative thereof per 100 g of the composition; about 300 mg to about 1700 mg of calcium per 100 g of the composition; about 70 mg to about 110 mg of vitamin C per 100 g of the composition; about 5 g to about 16 g of vitamin D per 100 g of the composition; and about 10 mg to about 20 mg of zinc per 100 g of the composition.

3. The composition of claim 1 further comprising about 15 g to about 30 g of protein per 100 g of the composition.

4. The composition of claim 1, wherein the composition is in the form of a powder.

5. The composition of claim 1, wherein the composition comprises a milk powder.

6. The composition of claim 1 comprising: about 3000 mg of glucosamine sulphate per 100 g of the composition; about 1700 mg of calcium per 100 g of the composition; about 70 mg of vitamin C per 100 g of the composition; about 16 g of vitamin D per 100 g of the composition; about 13 mg of zinc per 100 g of the composition, and about 22 g of protein per 100 g of the composition.

7. The composition of claim 1, wherein vitamin D is vitamin D.sub.3.

8. The composition of claim 1, wherein the composition provides: about 200 to about 500 Kcal per 100 g of the composition; about 15 g to about 30 g of protein per 100 g of the composition; about 5 g to about 25 g of fat per 100 g of the composition; about 40 g to about 60 g of carbohydrate per 100 g of the composition; and about 200 mg to about 400 mg of sodium per 100 g of the composition.

9. The composition of claim 1 in the form of a powdered food product.

10. A beverage product comprising a composition comprising: about 1500 mg to about 9000 mg of glucosamine or a pharmaceutically-acceptable derivative thereof per 100 g of the composition; about 300 mg to about 2000 mg of calcium per 100 g of the composition; about 50 mg to about 150 mg of vitamin C per 100 g of the composition; about 3 g to about 30 g of vitamin D per 100 g of the composition; and about 5 mg to about 25 mg of zinc per 100 g of the composition in the form of a powder which has been reconstituted in a liquid.

11. (canceled)

12. A method for maintaining or improving joint function mobility, and muscle strength in an ageing, healthy subject comprising administering a composition comprising: about 1500 mg to about 9000 mg of glucosamine or a pharmaceutically-acceptable derivative thereof per 100 g of the composition; about 300 mg to about 2000 mg of calcium per 100 g of the composition; about 50 mg to about 150 mg of vitamin C per 100 g of the composition; about 3 g to about 30 g of vitamin D per 100 g of the composition; and about 5 mg to about 25 mg of zinc per 100 g of the composition to the subject.

13. The method of claim 12, wherein the subject is aged over about 40 and/or is a pre-osteoarthritis subject.

14. The method of claim 12, wherein the method further comprises at least one physical activity.

15. (canceled)

16. The composition of claim 1, wherein the composition comprises about 7500 mg of glucosamine sulphate per 100 g of the composition; about 1350 mg of calcium per 100 g of the composition; about 110 mg of vitamin C per 100 g of the composition; about 7.5 g of vitamin D per 100 g of the composition; and about 20 mg of zinc per 100 g of the composition.

Description

DESCRIPTION OF THE DRAWINGS

[0051] FIG. 1 is a schematic representation of the typical range of motion for a knee joint. The range of motion is typically from 0 degrees of extension (completely straight knee) to 135 degrees of flexion (fully bent knee joint).

[0052] FIG. 2 compares the walking distance (in metres) achieved in the six minute walk test (6MWD) of Example 2 for low, median and high subjects at the baseline, after the nutrition period (V3) and at the end of the study (V7).

[0053] FIG. 3 compares the torque max flexor (nM) achieved in the dominant leg strength testing of Example 2 for low, median and high subjects at the baseline and at the end of the study (V7).

[0054] FIG. 4 compares the total work flexor (J) achieved in the dominant leg strength testing of Example 2 for low, median and high subjects at the baseline and at the end of the study (V7).

[0055] FIG. 5 shows the results of the balance outcome test in Example 2. This figure is a plot of balancing time (in minutes) against visit number (V1, V3, V4, V6 and V7) for both the left leg (O) and the right leg ().

[0056] FIG. 6 compares the EQ5D-5L scores to assess quality of life at baseline, visit 3 (end of nutrition period) and visit 7 (end of study).

DETAILED DESCRIPTION OF THE INVENTION

[0057] Various preferred features and embodiments of the present invention will now be described by way of non-limiting examples. It is noted that the various aspects, features, examples and embodiments described in the present application may be compatible and/or combined together. In particular, features, examples and embodiments described in the context of the composition of the invention are equally applicable to the uses and methods of the invention.

[0058] Unless otherwise indicated, all amounts indicated for nutrients are expressed as amounts per 100 g of the composition. For example, about 2500 mg/100 g of glucosamine sulphate means about 2500 mg glucosamine sulphate per 100 g of the composition.

[0059] Ageing, Healthy Subject

[0060] The subjects referred to in the present disclosure as the target of the compositions are ageing, healthy subjects. Preferably these subjects are human.

[0061] By ageing is meant that the subject is over the age of 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100 years old. Preferably the subject is over about 40, more preferably over about 50, even more preferably over about 60 years old.

[0062] In one embodiment the subject is aged between about 40 and about 70 years, preferably between about 45 and about 70 years, more preferably between about 45 and about 65 years.

[0063] By healthy is meant that the subject has not been physician-diagnosed with a musculoskeletal disorder. Musculoskeletal disorders (MSDs) are conditions that can affect your muscles, bones and joints. They are also conditions which have a higher risk of developing with age.

[0064] Non-limiting examples of musculoskeletal disorders include tendinitis, epicondylitis, carpal tunnel syndrome, osteoarthritis, rheumatoid arthritis, fibromyalgia, bone fractures, osteoporosis, and gout. In one embodiment the subject has not been diagnosed by a physician as having osteoarthritis.

[0065] In one embodiment the subject may experience ill-effects associated with ageing. Non-limiting examples of such ill-effects include joint discomfort, joint stiffness, joint pain, muscular pain, vision problems, mobility issues, cognitive issues, lack of bone density, cardiovascular risks, bone pain, high blood fat/cholesterol, lack of energy/fatigue, urinary issues, high blood sugar and weight gain.

[0066] In one embodiment the subject may suffer from one or more ill-effects associated with the joints such as joint discomfort, joint stiffness or joint pain, preferably the subject may suffer from joint discomfort, e.g. knee, hand (including finger and thumb), and/or hip joint(s) discomfort.

[0067] In a preferred embodiment, ageing, healthy subject refers to a female or male human subject aged over about 40 old, who has not been diagnosed by a physician as having osteoarthritis. In a particularly preferred embodiment, the subject suffers from one or more of joint discomfort, joint stiffness or joint pain, preferably one or more of joint discomfort or joint pain, more preferably joint discomfort.

[0068] These subjects are referred to herein as pre-osteoarthritis subjects. They are subjects which have not been physician-diagnosed with osteoarthritis but show at least one ill-effect associated with a joint, e.g. the knee joint, the hand joints or the hip joint.

[0069] Glucosamine

[0070] The skilled person will have a detailed and thorough understanding of the use of glucosamine in nutritional compositions. Briefly, glucosamine is an oral dietary supplement which is marketed to support the structure and function of joints.

[0071] The compositions of the invention include glucosamine or a pharmaceutically acceptable derivative thereof. By derivative is meant any pharmaceutically acceptable salt, solvate and/or reaction product thereof which does not affect the activity of glucosamine. In one embodiment glucosamine or a pharmaceutically acceptable derivative thereof means glucosamine, glucosamine sulphate, glucosamine hydrochloride or N-acetylglucosamine. N-acetylglucosamine is for instance an amide formed by the reaction of glucosamine and acetic acid. Preferably the composition includes glucosamine sulphate.

[0072] The composition includes about 1500 mg to about 9000 mg of glucosamine or a pharmaceutically acceptable derivative thereof per 100 g of the composition.

[0073] In one embodiment the composition includes about 1500 mg to about 8000 mg, about 2000 mg to about 8000 mg, about 2500 mg to about 8000 mg, about 2500 mg to about 7500 mg, about 3000 mg to about 7500 mg, about 3000 mg to about 7000 mg, about 3500 mg to about 6000 mg, or about 4000 to about 6000 mg of glucosamine or a pharmaceutically acceptable derivative thereof per 100 g of the composition.

[0074] In a preferred embodiment the composition includes about 1500 mg to about 8000 mg of glucosamine or a pharmaceutically acceptable derivative thereof per 100 g of the composition. For example about 1500 mg or about 2000 mg or about 2500 mg or about 3000 mg or about 3500 mg or about 4000 mg or about 4500 mg or about 5000 mg or about 5500 or about 6000 mg or about 6500 mg or about 7000 mg or about 7500 mg or about 8000 mg of glucosamine or a pharmaceutically acceptable derivative thereof per 100 g of the composition.

[0075] In one embodiment the composition includes about 2500 mg to about 8000 mg of glucosamine or a pharmaceutically acceptable derivative thereof per 100 g of the composition. In one embodiment the composition includes about 3000 mg of glucosamine or a pharmaceutically acceptable derivative thereof per 100 g of the composition.

[0076] In another embodiment the composition includes about 7500 mg of glucosamine or a pharmaceutically acceptable derivative thereof per 100 g of the composition.

[0077] Another way of defining the composition is that glucosamine or a pharmaceutically acceptable derivative thereof is comprised therein in an amount such that the total daily intake derived from the composition of the invention is about 750 mg to about 3000 mg, preferably about 1000 mg to about 2500 mg, most preferably about 1500 mg.

[0078] Preferably, the pharmaceutically acceptable derivative of glucosamine is glucosamine sulphate.

[0079] Calcium

[0080] Calcium may be present in the composition of the invention as a component of the milk powder and/or as an independent component.

[0081] Calcium may for example be incorporated in the composition of the invention as such or in the form of a physiologically acceptable salt and/or via any source comprising calcium. For example calcium carbonate, calcium chloride, calcium salts of citric acid, calcium gluconate, calcium glycerophosphate, calcium lactate, calcium hydroxide, or calcium salts of orthophosphoric acid may be used.

[0082] Preferably calcium is at least incorporated in the composition of the invention in the form of calcium carbonate.

[0083] More preferably calcium is incorporated in the composition of the invention as part of the milk powder and independently in the form of calcium carbonate.

[0084] The composition includes about 300 mg to about 2000 mg of calcium per 100 g of the composition. When the composition includes a milk powder the composition may include about 900 mg to about 2000 mg of calcium per 100 g of the composition.

[0085] In one embodiment the composition includes about 300 mg to about 1700 mg, about 400 mg to about 1600 mg, about 500 mg to about 1500 mg, about 600 mg to about 1500 mg, about 700 mg to about 1500 mg, about 800 mg to about 1500 mg, about 900 mg to about 1450 mg, about 1000 mg to about 1400 mg, about 1100 mg to about 1300 mg of calcium per 100 g of the composition.

[0086] In one embodiment the composition includes about 1300 mg to about 1700 mg, about 1350 mg to about 1650 mg, about 1400 mg to about 1650 mg, or about 1400 mg to about 1600 mg of calcium per 100 g of the composition.

[0087] In a preferred embodiment the composition includes about 1350 mg to about 1700 mg of calcium per 100 g of the composition. For example about 1350 mg or about 1375 mg or about 1400 mg or about 1425 mg or about 1450 mg or about 1475 or about 1500 mg or about 1525 mg or about 1550 mg or about 1575 mg or about 1600 mg or about 1625 mg or about 1650 mg or about 1675 mg or about 1700 mg of calcium per 100 g of the composition.

[0088] In one embodiment the composition includes about 1700 mg of calcium per 100 g of the composition.

[0089] In another embodiment the composition includes about 1350 mg of calcium per 100 g of the composition.

[0090] Alternatively, calcium is comprised in the composition in an amount such that the total daily intake derived from the composition of the invention is from about 100 to about 2500 mg, more preferably from about 200 to about 2000 mg, more preferably, from about 250 to about 1500 mg, even more preferably from about 500 to about 1000 mg.

[0091] In a preferred embodiment, the composition according to the present invention comprises levels of calcium such that the total daily intake derived from the composition of the invention is about 850 mg.

[0092] Vitamin D

[0093] In one embodiment the composition includes vitamin D in the form of vitamin D.sub.3, also known as cholecalciferol. In another embodiment the composition includes vitamin D in the form of vitamin D.sub.2, also known as ergocalciferol.

[0094] Preferably the vitamin D is vitamin D.sub.3.

[0095] The composition includes about 3 g to about 30 g of vitamin D per 100 g of the composition.

[0096] In one embodiment the composition includes about 5 g to about 25 g, about 5 g to about 22.5 g, about 5 g to about 16 g, about 7.5 g to about 22.5 g, about 7.5 g to about 20 g, about 7.5 g to about 17.5 g, about 7.5 g to about 16 g, about 7.5 g to about 15 g, about 10 g to about 12.5 g of vitamin D per 100 g of the composition.

[0097] In a preferred embodiment the composition includes about 5 g to about 16 g per 100 g of dry composition. For example about 5 g or about 5.5 g or about 6 g or about 7 g or about 7.5 g or about 8 g or about 8.5 g or about 9 g or about 9.5 g or about 10 g or about 10.5 g or about 11 g or about 11.5 g or about 12 g or about 12.5 g or about 13 g or about 13.5 g or about 14 g or about 14.5 g or about 15 g or about 15.5 g or about 16 g of vitamin D per 100 g of the composition.

[0098] In one embodiment the composition includes about 16 g of vitamin D per 100 g of the composition.

[0099] In another embodiment the composition includes about 7.5 g of vitamin D per 100 g of the composition.

[0100] Alternatively the composition comprises an amount of vitamin D such that the total daily intake derived from the composition of the invention is from about 0.2 to about 30 g, more preferably from about 0.5 to about 25 g, more preferably from about 0.5 to about 15 g, even more preferably from about 1 to about 10 g, more preferably still, from about 2 to about 8 g.

[0101] In one highly preferred embodiment, the composition comprises an amount of vitamin D such that the total daily intake derived from the composition of the invention is about 8 g.

[0102] Vitamin C

[0103] The composition includes about 50 mg to about 150 mg of vitamin C per 100 g of the composition.

[0104] In one embodiment the composition includes about 50 mg to about 125 mg, about 55 mg to about 125 mg, about 55 mg to about 120 mg, about 60 mg to about 120 mg, about 60 mg to about 115 mg, about 65 mg to about 115 mg, about 65 mg to about 110 mg, about 70 mg to about 110 mg, about 70 mg to about 100 mg, about 75 mg to about 100 mg, about 75 mg to about 95 mg, or about 80 mg to about 95 mg of vitamin C per 100 g of the composition.

[0105] In a preferred embodiment the composition includes about 70 mg to about 110 mg of vitamin C per 100 g of dry composition. For example about 70 mg or about 75 mg or about 80 mg or about 85 mg or about 90 mg or about 95 mg or about 100 mg or about 105 mg or about 110 mg of vitamin C per 100 g of the composition.

[0106] In one embodiment the composition includes about 70 mg of vitamin C per 100 g of the composition.

[0107] In another embodiment the composition includes about 110 mg of vitamin C per 100 g of the composition.

[0108] Alternatively the composition comprises an amount of vitamin C such that the total daily intake derived from the composition of the invention is from about 5 to about 50 mg, more preferably from about 10 to about 45 mg, more preferably from about 15 to about 40 mg, even more preferably from about 20 to about 40 mg.

[0109] In one highly preferred embodiment, the composition comprises an amount of vitamin C such that the total daily intake derived from the composition of the invention is about 35 mg.

[0110] Zinc

[0111] Zinc may be incorporated in the compositions of the invention in the form of a physiologically acceptable salt such as for example: zinc nitrate, zinc sulfate, zinc gluconate, zinc acetate or mixtures thereof, or in the form of a physiologically acceptable zinc complex (such as for example zinc picolinate) or mixtures thereof.

[0112] The composition includes about 5 mg to about 25 mg of zinc per 100 g of the composition.

[0113] In one embodiment the composition includes about 8 mg to about 25 mg, about 10 mg to about 25 mg, about 11 mg to about 25 mg, about 11 mg to about 24 mg, about 12 mg to about 23 mg, about 12 mg to about 22 mg, about 13 mg to about 21 mg, about 13 mg to about 20 mg, about 14 mg to about 19 mg or about 15 mg to about 18 mg of zinc per 100 g of the composition.

[0114] In a preferred embodiment the composition includes about 10 mg to about 20 mg of zinc per 100 g of the composition. For example about 10 mg or about 11 mg or about 12 mg or about 13 mg or about 14 mg or about 15 mg or about 16 mg or about 17 mg or about 18 mg or about 19 mg or about 20 mg of zinc per 100 g of the composition.

[0115] In one embodiment the composition includes about 13 mg of zinc per 100 g of the composition.

[0116] In another embodiment the composition includes about 20 mg of zinc per 100 g of the composition.

[0117] Alternatively the composition comprises an amount of zinc such that the total daily intake derived from the composition of the invention is from about 1 to about 25 mg, more preferably from about 2 to about 20 mg, more preferably from about 4 to about 10 mg, even more preferably from about 5 to about 8 mg.

[0118] In one highly preferred embodiment, the zinc is comprised in the composition in an amount such that the total daily intake derived from the composition is about 6.5 mg.

[0119] Protein

[0120] In one embodiment the composition of the invention includes protein.

[0121] The term protein refers to polymers of amino acids, and includes polypeptides and peptides. The term protein does not encompass free amino acids, which may also be present in the composition of the invention.

[0122] The protein may be included at an amount of about 15 g to about 30 g per 100 g of the composition. In one embodiment the protein is included at an amount of about 16 to about 25 g or about 17 to about 24 g or about 18 to about 23 g or about 20 to 23 g of protein per 100 g of the composition.

[0123] In a preferred embodiment the composition includes about 22 g of protein per 100 g of the composition.

[0124] Any suitable dietary protein may be used for example animal proteins (such as milk proteins, meat proteins and egg proteins); vegetable proteins (such as soy protein, wheat protein, rice protein, and pea protein); or combinations thereof.

[0125] Non-limiting examples of proteins include casein, alpha-lactalbumin, whey, soy protein, rice protein, corn protein, oat protein, barley protein, wheat protein, rye protein, pea protein, egg protein, sunflower seed protein, potato protein, fish protein, meat protein, lactoferrin, serum albumin, immunoglobins, and combinations thereof.

[0126] Particularly preferred are milk proteins such as casein and whey, and soy proteins. Milk proteins, such as casein and whey, are the most preferred.

[0127] In one embodiment the protein is present when the composition comprises a milk powder.

[0128] Milk Powder

[0129] In one embodiment the composition of the invention comprises a milk powder.

[0130] Any milk powder known in the art can be included, provided that the overall composition meets the content specification of the invention. Non-limiting examples of the milk powder include skimmed milk powder, whole milk powder, non-fat dry milk, yoghurt powder and reduced fat milk powder.

[0131] In one embodiment the milk powder is a standardised milk powder prepared by spray-drying solid non-fat, milk fat, a bulking agent and an emulsifier. The bulking agent may be maltodextrin, e.g. maltodextrin DE 37-41, and the emulsifier may be lecithin.

[0132] Additional Components

[0133] The composition of the invention may include additional components or excipients known to be employed in the type of composition in question. Such additional components will be familiar to the skilled person in the art.

[0134] The composition may for example comprise additional minerals, vitamins, salts, functional additives including, for example, palatants, colorants, emulsifiers, antimicrobial or other preservatives. Minerals that may be useful in such compositions include, for example, phosphorous, potassium, sodium, iron, chloride, boron, copper, magnesium, manganese, iodine, selenium, chromium, molybdenum, fluoride and the like. Minerals are usually added in their salt form.

[0135] Examples of additional vitamins that may be useful in compositions described herein include water soluble vitamins (such as thiamin (vitamin B1), riboflavin (vitamin B2), niacin (vitamin B3), pantothenic acid (vitamin B5), pyridoxine (vitamin B6), biotin (vitamin B7), myo-inositol (vitamin B8), folic acid (vitamin B9), and cobalamin (vitamin B12)) and fat soluble vitamins (such as vitamin A, vitamin E, and vitamin K) including salts, esters or derivatives thereof. Inulin, taurine, carnitine, amino acids, enzymes, coenzymes, and the like may be useful to include in various embodiments.

[0136] The skilled person in the art can identify appropriate amounts of the above mentioned additional components based on, for example, the nature and purpose of the composition, the target subject and the dosage of the composition.

[0137] The composition may also contain minerals and micronutrients such as trace elements and vitamins in accordance with the recommendations of Government bodies such as the USRDA or the Chinese RDA. For example, the compositions may also contain one or more vitamins and minerals understood to be essential in the daily diet and in nutritionally significant amounts.

[0138] Preferably, the composition does not contain hydrocolloids, such as gelatine, modified starch, xanthan gum, carrageenan, or gellan gum.

[0139] Type of Composition

[0140] The composition may be any type of composition suitable for administration to an ageing, healthy human subject. Compositions of the invention are preferably in solid form. The composition may, for example, be in the form of a tablet, drage, capsule, gel cap, powder, granule, solution, emulsion, suspension, coated particle, spray-dried particle or pill.

[0141] In another embodiment the composition may be in the form of a powder. The powder may, for example, be a spray-dried powder, a freeze-dried powder or a dry-mixed powder.

[0142] A powder composition may be contained in a sachet or a container.

[0143] In one embodiment the powder composition is contained in a multi-serving container.

[0144] In another embodiment the powder composition is contained in a sachet in an amount suitable for one serving.

[0145] The term serving refers to a total amount of composition which can deliver about 750 mg of glucosamine, about 17.5 mg of vitamin C, about 425 mg of calcium, about 4 g of vitamin D, about 3.25 mg zinc and about 5.5 g protein. In one embodiment a serving is about 20 g to about 50 g, preferably about 20 g to about 30 g, most preferably about 25 g of the composition.

[0146] A powder composition according to the present invention may be used to sprinkle onto a food or beverage. More preferably the composition is in the form of a powder for reconstituting in a liquid.

[0147] A particularly preferred embodiment provides a composition according to the invention in the form of a sachet containing a powder, wherein the powder can be dispersed or dissolved into a beverage (e.g. water, fruit juice, milk, etc.) to provide a palatable nutrient liquid for oral administration.

[0148] In one embodiment the composition is in the form of a powdered food product.

[0149] In another embodiment the composition is in the form of a beverage product, where the beverage comprises the composition in the form of a powder reconstituted with a liquid. Preferably the liquid is water.

[0150] Example food or beverage products include a dairy product, a dairy dessert, a milk drink, cream, ice cream mix, and soya product. When the composition comprises a milk powder, the beverage product may be a milk drink. This milk drink is preferably prepared by reconstituting the powder composition described herein in liquid, e.g. water.

[0151] Nutritional Composition

[0152] The composition may be in the form of a nutritional composition or a nutritional supplement.

[0153] The term nutritional supplement refers to a product which is intended to supplement the general diet of a subject.

[0154] The composition may be in the form of a complete nutritional product. The term complete nutritional product refers to a product which is capable of being the sole source of nourishment for the subject, for example including a source of protein, carbohydrate and fat.

[0155] In one preferred embodiment of the present invention, the composition comprises one or more of a protein source, a fat source and a carbohydrate source. Each of these sources may be any suitable source known in the art.

[0156] The carbohydrate content of the composition of the invention is preferably in the range about 30 to about 70 g per 100 g of the composition, more preferably about 40 to about 60 g per 100 of the composition. In a particular preferred embodiment the carbohydrate content of the composition is about 53 g per 100 g of the composition.

[0157] Example carbohydrates include lactose, saccharose, maltodextrin and starch. Mixtures of carbohydrates may be used. In one embodiment the carbohydrate comprises maltodextrin. In one embodiment, the composition comprises at least 5, 10, 15 or 20% of maltodextrin, wherein the % is based on the solids content of the composition.

[0158] In one embodiment the carbohydrates comprises lactose. In one embodiment the composition comprises at least 40%, 50%, 60% or 70% solids content of lactose.

[0159] In one embodiment the carbohydrate comprises lactose and maltodextrin.

[0160] The fat content of the composition of the invention is preferably in the range of about 5 to about 20 g per 100 g of the composition, more preferably in the range of about 10 to about 15 g per 100 g of the composition. In a particularly preferred embodiment the fat content of the composition is about 12 g per 100 g of the composition.

[0161] The fat may be any lipid or fat which is suitable for use in the composition. In one embodiment the fat comprises milk fat.

[0162] Mobility Benefits

[0163] The compositions, uses and methods of the invention provide mobility benefits. In particular, the compositions, uses and methods of the invention provide for the maintenance or improvement of joint function and muscle strength in an ageing, healthy subject.

[0164] The term mobility refers to the ability of an individual to move.

[0165] The maintenance or improvement in mobility can be measured by various methods known in the art and illustrated in the examples below.

[0166] Suitable methods for assessing mobility include gait speed, the 6 minute walking distance test, short physical performance battery and self-reported mobility. All of these methods are known in the art. The 6 minute walking test is for instance described in Macias-Hernandez, S. I. et al., Clinical Rheumatology, Volume 35, Issue 8, 1 Aug. 2016, Pages 2087-2092 which is incorporated herein by reference.

[0167] The term joint functionality or joint function refers to the ability of a joint to move in its full range of motion, bear weight and perform work.

[0168] As is known in the art, individual joints have a predetermined range of motion. This range of motion is commonly measured in degrees. For example, hips, knees, ankles, feet, joints of the feet, joints of the toe, shoulders, elbows, wrists, and hand and finger joints all have different ranges of motion, and there are generally accepted values for a normal range of motion in each of these joints. These values would be known to a skilled person in the art.

[0169] The maintenance or improvement in joint function and muscle strength can be measured by various methods known in the art and illustrated in the examples below.

[0170] A suitable method for assessing joint function and muscle strength is a dominant leg strength assessment using an isokinetic dynamometer. This assessment can, for example, include measuring isokinetic parameters such as torque max flexor, torque max extensor, total work flexor, total work extensor, and ratios thereof.

[0171] The term maintain, maintains or maintaining refers to a particular parameter, such as mobility, remaining substantially unchanged over a period of time (e.g. 5, 10, 15, 20, 25, 30, 40, 50 or more years).

[0172] In one embodiment, the mobility of a subject increases by at least about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 10%, about 15% or about 20%.

[0173] In another embodiment, the mobility of a subject increases by about 1 to about 6%, about 1 to about 10% or about 1 to about 20%.

[0174] In a preferred embodiment the mobility of a subject increases by at least about 6% measured according to the 6 minute walking distance test (6MWD).

[0175] In one embodiment the joint function and muscle strength of a subject increases by at least about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 10%, about 15% or about 20%.

[0176] In another embodiment, the joint function and muscle strength of a subject increases by about 1 to about 6%, about 1 to about 10% or about 1 to about 20%.

[0177] Preferably the muscle is skeletal muscle.

[0178] In one embodiment the increase in joint function and muscle strength is determined with an isokinetic dynamometer.

[0179] Physical Activity

[0180] In one embodiment the composition for use according to the invention includes at least one physical activity. By physical activity is meant any bodily movement produced by skeletal muscles that requires energy expenditure. This is the definition given by the World Health Organisation.

[0181] Non-limiting examples of physical activity include strength exercises, Tai-Chi, balance exercises, aerobic exercises such as walking, dancing, jogging, running, cycling or participating in sports, and flexibility exercises such as yoga, stretching and Pilates.

[0182] As explained hereinabove, the present invention advantageously provides a composition which can be combined with at least one physical activity to maintain or improve joint mobility. The physical activity can form part of an exercise programme which may be tailored to the needs of the subject in question.

[0183] In a preferred embodiment the at least one physical activity is carried out at least 2 to 4 times a week, most preferably at least 3 times a week. For example, Tai Chi may be carried out once a week together with strength exercises twice a week. Aerobic exercises may also be carried every day.

[0184] In another preferred embodiment the at least one physical activity comprises walking for at least 130 minutes per week.

[0185] It should be appreciated that all features of the present invention disclosed herein can be freely combined and that variations and modifications may be made without departing from the scope of the invention as defined in the claims. Furthermore, where known equivalents exist to specific features, such equivalents are incorporated as if specifically referred to in this specification.

[0186] There now follows a series of non-limiting examples that serve to illustrate the invention.

EXAMPLES

Example 1

[0187] Preparation of the composition, powdered food product and beverage product

[0188] A milk powder was prepared by spray drying, according to a method known in the art, the following mixture of components:

TABLE-US-00001 Components Percent (%, solid) SNF (Solid Non Fat = lactose, proteins 65.821 and minerals from milk) Milk FAT 11.544 Maltodextrin DE (37-41) (=bulking agent) 11.178 Lecithin (emulsifier) 0.321

[0189] The spray-dried milk powder was then dry-mixed with the following nutrients to form a powder:

TABLE-US-00002 Components Percent (%, solid) Calcium carbonate 2.150 Glucosamine sulphate 4.320 Vitamin Mineral Premix 0.950 (including zinc, vitamin C and vitamin D.sub.3) Flavour 0.126 Milk Powder 0.090 Water 3.500 TOTAL 100.000

[0190] The nutritional analysis of the product was as follows:

TABLE-US-00003 Energy Kcal/100 g 406 Protein g/100 g 22 Fat g/100 g 12 Carbohydrates g/100 g 53 Na mg/100 g 360 Vitamin D3 g/100 g 16 Vitamin C mg/100 g 70 Ca mg/100 g 1700 Zn mg/100 g 13 Glucosamine/GS mg/100 g 3000 Serving Size 25 g

[0191] To prepare a beverage product according to the present invention, the 25 g of powder was reconstituted in approximately 180 ml of water.

[0192] When taken twice a day, this product provided 1500 mg of glucosamine sulphate, 6.5 mg of zinc, 850 mg of calcium, 35 mg of vitamin C, and 8 g of vitamin D3, daily. The product also provided 203 Kcal, 11 g protein, 6 g fat, 26.5 g carbohydrates and 180 mg sodium, daily.

Example 2

[0193] Combining nutritional supplementation and a physical activity programme in healthy 50 year+ volunteers with knee joint discomfort

[0194] The aim of this example was to evaluate the efficacy of a 6-month intervention comprising the composition of the invention and a physical activity programme on outcomes in ageing, healthy pre-osteoarthritis volunteers.

[0195] Materials and Methods

[0196] This example is based on a single-centre, single arm, baseline-controlled, open label, nutrition and exercise intervention clinical trial. A total of 54 healthy subjects with knee joint discomfort were recruited, aged 45-65 years, and each volunteer was provided with a composition according to the present invention to be taken orally twice a day for 6 months.

[0197] The composition provided about 1500 mg of glucosamine sulphate, about 850 mg of calcium, about 35 mg of vitamin C, about 8 g of vitamin D (D.sub.3), about 6.5 mg of zinc and about 11 g of protein, daily. The milk-based drink was prepared by pouring 180 ml of warm water into a container and stirring 25 g of the milk powder containing the nutrient mix therein. This preparation was repeated twice a day.

[0198] Initially the composition was taken alone, i.e. without physical activity, for 2 months. It was then combined with an exercise programme for 4 months. The physical activity/exercise programme included a combination of Tai Chi, strength exercises and aerobic exercises (walking), 3 times per week.

[0199] Daily step counts were recorded using a pedometer (Garmin).

[0200] The first primary outcome was mobility. This was assessed using a validated physical function test: the 6 minute walking distance test (6MWD).

[0201] The second primary outcome was joint function and muscle strength. This was assessed using another validated physical function test: a dominant leg strength assessment using an isokinetic dynamometer. This assessment involved measuring various isokinetic parameters (torque max flexor, total work flexor, torque max extensor, total work extensor, ratio of torque flexor/extensor) using the dynamometer.

[0202] The secondary outcomes were assessed with a KOOS score (Knee injury and Osteoarthritis Outcome Score), a Visual Analog Scale (VAS) score, a balance test, biomarkers for bone and joint metabolism and a quality of life score (evaluated by an EQSD-5L questionnaire).

[0203] Assessments were conducted at baseline (V1), at 1 month (V2), at 2 months after the nutrition only period (V3), and then every month for the remaining time of the study (V4 to V7). Subjects were classified into three levels based on their average daily step count:

low<=9999;10,000<=average<=13999; and high>=14000.

[0204] Baseline parameters were compared to 6 months of intervention using an Analysis of Covariance (ANCOVA) model with the baseline value as covariate and levels of step counts as fixed effect.

[0205] To support the main statistical analysis, a longitudinal analysis of change from baseline at each visit was performed using Mixed Model Repeated Measures (MMRM). Results were obtained for the expected change from baseline for outcomes at each visit for three possible baseline quartile (Q) values, i.e. Q1, Q2 as median, and Q3 of the outcome at baseline. A linear model was used for the analysis.

[0206] Results: Primary Outcomes

[0207] 6 Minute Walking Distance Test (6MWD)

[0208] FIG. 2 shows the increase in walking distance for each sub-group of subjects at baseline, V3 (end of nutrition period) and V7 (end of the study). It can be seen from this figure that the walking distance significantly improved by approximately 6% in all subjects at the end of the study; the change from baseline to 6 months was significantly increased for each level of step count (p<0.001).

[0209] The supportive statistical analysis of the 6MWD results using Mixed Model Repeated Measures also showed that for Q1, the change from baseline at each visit was statistically significant following the nutrition period alone (p<0.05). In other words, the improvement in walking distance was observed after nutrition alone in the lowest scores at baseline.

[0210] Dominant Leg Strength

[0211] FIG. 1 shows the typical range of motion for the knee joint. Flexion refers to the strength to flex the leg and extension refers to the strength to extend the leg.

[0212] The isokinetic parameters determined to assess dominant leg strength (and hence joint function and muscle strength) were torque max flexor, total work flexor, torque max extensor, total work extensor and the ratio of torque flexor/extensor.

[0213] The change from baseline for each of these parameters at V7 (i.e. the end of the study) are shown in Table 1 below.

TABLE-US-00004 TABLE 1 Isokinetic Parameter Estimate Std. Error 2.5% 97.50% P-value Torque Max Extensor (Nm) Low step count 0.10 0.07 0.06 0.27 0.1535 Average step count 0.05 0.07 0.12 0.22 0.5192 High step count 0.06 0.10 0.17 0.29 0.5141 Torque Max Flexor (Nm) Low step count 0.11 0.04 0.04 0.19 0.0042 Average step count 0.06 0.04 0.02 0.13 0.1388 High step count 0.12 0.05 0.02 0.23 0.0190 Total Work Extensor (J) Low step count 0.49 0.40 0.32 1.30 0.2289 Average step count 0.49 0.41 0.34 1.31 0.2434 High step count 0.38 0.55 0.73 1.49 0.4896 Total Work Flexor (J) Low step count 0.51 0.22 0.06 0.96 0.0278 Average step count 0.26 0.23 0.20 0.73 0.2549 High step count 0.57 0.31 0.05 1.19 0.0724 Ratio Torque Flexor/Extensor Low step count 0.13 0.06 0.00 0.26 0.0584 Average step count 0.06 0.07 0.07 0.20 0.3531 High step count 0.17 0.09 0.01 0.35 0.0666 The ANCONA model will include baseline isokinetic strength parameter value as covariate and levels of Garmin compliance as fixed effect. For Ratio Torque flexor/extensor), natural log of the ratio is taken and then change is calculated for log transformed values due to non-normality of the data. Analysis for Torque max extensor is performed at 2.5% level of significance and for rest of the parameters significance level is 5%.

[0214] FIG. 3 plots the mean values (nM) for the torque max flexor at baseline and at the end of the study (V7) for each step-count (all values were divided by the subject's weight (in kg) at each visit). These values, together with the results in Table 1 above, show that flexor maximum torque was significantly increased following the 6-month intervention in both those with low and high step counts (p<0.05).

[0215] FIG. 4 plots the mean values (J) for the total work flexor at baseline and at the end of the study (V7) for each step-count (all values were divided by subject's weight (in kg) at each visit). These values, together with the results in Table 1 above, show that total work flexor was significantly increased following the 6-month intervention in those with low step counts (p<0.05).

[0216] In summary, the main effects were on the flexion (strength in flex leg) and predominantly in low step-count volunteers. Some improvements were also seen after the nutritional intervention period alone (p<0.05).

[0217] The other isokinetic parameters tested (torque max extensor, total work extensor and ratio torque flexor/extensor) were found to be statistically non-significant for each level of step count.

[0218] Looking at the effect of the nutritional composition alone, however, the torque ratio (flexor/extensor) was significantly improved following 2 months of intervention in all subjects (p<0.05). This can be seen from Table 2 below as the change from baseline at visit 3 was statistically significant (p<0.05) for all quartiles.

TABLE-US-00005 TABLE 2 Mixed model results for the Isokinetic Parameters. Estimates, standard errors and 95% CI for change from baseline at each visit are presented for Q1, median and Q3 of baseline (Full Analysis Set) Quartiles of Isokinetics at Std. Isokinetic Parameters Visit Baseline Estimate Error 2.5% 97.5% P-value Ratio Torque Flexor/Extensor Visit 3-visit 1 First Quartile 0.28 0.04 0.19 0.36 <.0001 Median 0.11 0.03 0.05 0.18 0.0009 Third Quartile 0.08 0.04 015 0.00 0.0481 Torque Max Extensor (Nm) Visit 3-visit 1 First Quartile 0.09 0.07 0.04 0.22 0.1799 Median 0.10 0.05 0.20 0.00 0.0406 Third Quartile 0.17 0.06 0.29 0.06 0.0030 Total Work Extensor (J) Visit 3-visit 1 First Quartile 0.45 0.24 0.02 0.93 0.0605 Median 0.25 0.19 0.63 0.13 0.1909 Third Quartile 0.64 0.21 1.07 0.22 0.0031 Torque Max Flexor (Nm) Visit 3-visit 1 First Quartile 0.09 0.03 0.03 0.14 0.0021 Median 0.03 0.02 0.01 0.07 0.1609 Third Quartile 0.01 0.02 0.05 0.04 0.7666 Total Work Flexor (J) Visit 3-visit 1 First Quartile 0.39 0.15 0.09 0.68 0.0099 Median 0.05 0.12 0.18 0.29 0.6563 Third Quartile 0.32 0.14 0.59 0.04 0.0238

[0219] Results: Secondary Outcomes

[0220] Balance Test

[0221] For the balance test, subjects were classified according to total recorded time of balance training (minutes). The following levels were considered: [0222] Low balance training: 0<=Total time of balance training<=320 [0223] High balance training: Total time of balance training>=321

[0224] FIG. 5 is a boxplot of the balance test scores showing the balance times for the left leg and the right leg at baseline (V1), after the nutrition period (V3), at each month of the physical activity programme (V4, V5, V6) and at the end of the study (V7).

[0225] This figure shows that from the baseline to V7 there was an increase of approximately 36% (left leg) and approximately 41% (right leg) in the standing time on one leg in both low and high balance training time. In other words, balance outcomes were improved in all subjects at the end of the intervention (p<0.001). This is consistent with the results for the isokinetic flexion parameters.

[0226] MMRM analysis (not shown) demonstrated that nutritional intervention alone led to an improvement in the lowest score groups at baseline (Q1 and Q2).

[0227] Knee Pain (VAS)

[0228] Knee pain in subjects was measured using the Visual Analog Scale (VAS). The Visual Analog Scale is a psychometric response scale from 0 to 10, where 0 is no pain ever, 2 is mild plan, 5-6 is moderate pain, 8-9 is severe pain and 10 is the worst pain.

[0229] MMRM analysis demonstrated that there was a significant decrease (p<0.01) in knee pain by approximately 42% at the end of the study in all subjects. This was already observed after nutrition alone in the people reporting higher pain score (Q2 and Q3).

[0230] Knee Injury and Osteoarthritis Outcome Score (KOOS)

[0231] KOOS is a questionnaire which is well known in the art for quantifying joint injury to osteoarthritis (see for example: Roos E M et al., Health Qual Life Outcomes 2003; 1:64).

[0232] The questionnaire consists of 5 subscales: pain, other symptoms, function in daily living (ADL), function in sport and recreation (Sport/Rec) and knee related Quality of Life (QOL). Standardized answer options are given, the previous week is the time period considered when answering the questions, each question is assigned a score from 0 to 4, and a normalized score (100 indicating no symptoms and 0 indicating extreme symptoms) is calculated for each subscale.

[0233] The volunteers were given the KOOS in this example, and the change from baseline to visit 7 for the scores is shown in Table 3 below along with the ANCOVA model analysis.

TABLE-US-00006 TABLE 3 KOOS Score Estimate Std. Error 2.5% 97.5% P-value KOOS Pain* Low step count 7.85 1.94 3.72 11.97 0.0010 Average step count 11.01 2.32 6.05 15.96 0.0003 High step count 11.43 1.96 7.25 15.61 <.0001 KOOS Symptoms Low step count 3.22 3.67 4.60 11.03 0.3940 Average step count 0.22 4.57 9.51 9.96 0.9616 High step count 0.70 3.51 6.78 8.19 0.8439 KOOS ADL* Low step count 8.34 1.92 4.24 12.44 0.0006 Average step count 8.12 2.46 2.87 13.37 0.0049 High step count 13.00 1.98 8.79 17.21 <.0001 KOOS Sport* Low step count 8.26 2.15 3.68 12.84 0.0016 Average step count 9.20 2.60 3.66 14.75 0.0030 High step count 12.31 2.13 7.76 16.85 <.0001 KOOS QOL Low step count 27.32 5.43 15.74 38.91 0.0002 Average step count 22.28 6.40 8.64 35.92 0.0033 High step count 20.15 5.31 8.85 31.46 0.0017 The ANCOVA model will include baseline KOOS score as covariate and levels of Garmin compliance as fixed effect.

[0234] It can be seen from this data that the change from baseline at V7 was statistically significant for KOOS pain (approximately 5.5% decrease), ADL (approximately 4% decrease), and sport (approximately 5.5% decrease) at each level of step count (p<0.005).

[0235] There was also a highly significant improvement in the quality of life score for all step counts: approximately 51% improvement from baseline (p<0.005).

[0236] Quality of Life: EQSD-5L

[0237] To further assess the effect on quality of life, volunteers were asked to complete the EQ5D-5L questionnaire. The change from baseline for the EQ5D-5L scores is shown in FIG. 6. The change from baseline to visit 7 was statistically significant at Q1 (p<0.005).

[0238] Biomarkers CTxl, CTX2 and P1NP

[0239] CTxl and P1NP are biomarkers of bone turnover; CTX2 is a biomarker of cartilage breakdown. These were monitored in the volunteers and at 6 months each of the biomarkers appeared to be positively modulated by the combined programme of nutrition and exercise (p<0.01). In particular there was a decrease of about 22% in cartilage breakdown and a decrease of about 20% in bone breakdown. Some effects were also already observed after nutrition alone.

CONCLUSION

[0240] This example demonstrates that improved mobility is achieved in healthy, ageing subjects with the composition of the present invention, and that this improvement is enhanced by combining this composition with physical activity.

[0241] This example also demonstrates that maintained or improved joint function and muscle strength is achieved in healthy, ageing subjects with the composition of the present invention, and that this effect is enhanced by combining this composition with physical activity.

[0242] This intervention programme particularly showed that a significant decrease in knee pain could be achieved (approximately 42% decrease in VAS) and that this led to an improved quality of life score (approximately 51% increase in KOOS-QoL) for participants.

[0243] All publications mentioned in the above specification are herein incorporated by reference. Various modifications and variations of the described compositions, uses and methods of the present invention will be apparent to those skilled in the art without departing from the scope and spirit of the present invention. Although the present invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention, which are obvious to those skilled in biochemistry and biotechnology or related fields, are intended to be within the scope of the following claims.