DEVICE FOR ISOLATING CELLS FROM TISSUE

Abstract

Provided is a device for rapidly isolating cells from tissue, comprising: a stirring and cutting member, having a rotary cutting tool, wherein same is driven by a motor so as to rotate to stir and cut tissue, and can inject digestive enzymes to accelerate decomposition of the tissue; and double-layered nested cups comprising an inner cup and an outer cup, used for accommodating and filtering solution of required cells, wherein a filter device of the inner cup screens out the required cells, and the outer cup collects the filtered solution.

Claims

1. A device for isolating cells from tissue, comprising a stirring and shearing component including a rotatable cutting tool and being arranged on a rotor linkage rod driven by a motor, and configured for cutting and stirring a tissue during decomposition in presence of a digestive enzyme solution; an inner set filtering cup for receiving the stirring and shearing component and a digestive enzyme solution and being provided with a sieve component configured for filtering the solution cut by the stirring and shearing component and containing the desired isolated cells; and an outer set cup for receiving inner set filtering cup and the filtered solution with the desired single cells.

2. The device of claim 1, wherein the rotatable cutting tool is a cutting stirring knife having a plurality of sandwich structures, the cutting stirring knife having multiple sandwich structures being provided with a rotating blade and a static blade, wherein the length of each rotating blade is smaller than the length of the respective static blade, wherein at least one stop member is located on the inner wall of the inner set filtering cup for blocking the static blades from rotating, when the cutting tool is rotated, so that the static blades are therefore not movable.

3. The device of claim 1, wherein the rotatable cutting tool comprises a plurality of rotating blades, which are vertically spaced from each other by a certain distance, and a plurality of static blades fixed on the inner wall of the inner set filtering cup, wherein each static blade is correspondingly arranged above or below a corresponding rotating blade, so as to form a cutting stirring knife with a sandwich structure.

4. The device of claim 1, wherein the sieve component of the inner set filtering cup consists of a plurality of filtering holes penetrating through the inner set filtering cup, wherein the diameters of the plurality of filter holes allow the required cells to pass therethrough, whereas tissue fragments not decomposed by the digestive enzyme solution are prevented to pass through the selected diameter.

5. The device of claim 1, wherein the sieve element of the inner set filtering cup consists of a plurality of filtering holes penetrating through the bottom of the inner set filtering cup and a screen mesh arranged at the bottom of the cup, wherein the mesh size of the screen mesh is smaller than that of the filter holes in the cup bottom, wherein the required cells are allowed to pass through the mesh, whereas the mesh retains tissue fragments not being decomposed by the digestive enzyme solution.

6. The device of claim 2, wherein the rotatable cutting tool is provided with a plurality of cutting blades, and the stirring shearing component is independently removable or is simultaneously removable with the inner set filtering cup.

7. The device of claim 2, wherein each of the cutting and stirring blades is formed by directly stacking a plurality of layers by a plurality of blades.

8. The device of claim 2, wherein the stopping component is cylindrical, semi-cylindrical or elongated strip-shaped.

9. The device of claim 3, wherein the static blades fixed on the inner wall of the inner set filtering cup are respectively mounted on the inner wall of the inner set filtering cup by means of a pin.

10. The device of claim 1, wherein the rotor of the motor is adjustable to perform a forward rotation or reverse rotation, and the rotation speed of the motor being less than 90 rpm.

11. The device of claim 1, wherein the stirring and shearing component has a base connected thereto, which is used for supporting the rotatable cutting tool.

Description

BRIEF DESCRIPTION OF THE DRAWING

[0015] The drawing for illustrating the invention by way of an example shows

[0016] FIG. 1 a schematic diagram of the overall structure of a device for isolating cells from tissue according to the present invention.

DETAILED DESCRIPTION OF THE SPECIFIC EMBODIMENTS

[0017] The present invention provides a device for rapidly isolating cells from tissue, which substantially comprises a stirring-shearing component for cutting of continuous tissues, wherein the stirring-shearing component is provided with a rotor having spiral blades, wherein an outer set cup is provided with a fixed cutting knife structure, wherein cutting and stirring of tissues are simultaneously thoroughly performed through the rotation of the spiral blades. During operation, a tissue and digestive enzyme solution are simultaneously placed into the device. Through the cutting and stirring and by means of the effects of the digestive enzymes, the block-shaped tissue can be effectively cut into small pieces, so that the contact with the digestive enzymes can be enhanced. The digestive enzymes can digest the tissue, so that the cells can be released from the tissue. The cell tissue suspended in the solution can pass through the filtering member of the inner set cup. After the digestion is completed, the movable spiral cutting blades and the inner set filtering cup are removed. The cell tissue is accommodated in the outer set cup, so that collection of the cells is completed.

[0018] Reference is made to FIG. 1. In the present embodiment, a device for isolating cells from tissue 100 according to the present invention comprises the following:

[0019] a stirring and shearing component 110 including a rotatable cutting tool 111 and being arranged on a rotor linkage rod 120, which is driven by a motor (not shown in the FIGURE), and serving for cutting and stirring the tissue during decomposition, when the digestive enzyme solution is added;

[0020] an inner set filtering cup 130 for receiving the stirring and shearing component 110 and a digestive enzyme solution and comprising a sieve component (not shown in the FIGURE) for filtering a solution containing the desired cells cut by the stirring and shearing component 110; and

[0021] an outer set cup 140 for accommodating the inner set filtering cup 130 and the filtered solution with the required single cells.

[0022] In the device of the present invention, the rotatable cutting tool 111 is a cutting stirring blade having a plurality of sandwich structures. The cutting stirring blade having a plurality of sandwich structures is respectively provided with a rotating blade 1111 and a static blade 1112, the length of each rotating blade 1111 being smaller than that of the respective static blade 1112, wherein, when the cutting tool 1111 is rotated, each of the static blades 1112 is prevented from rotating by at least one stop member 131 located on the inner wall of the inner set filtering cup 130, so that the static blades 1112 are not movable. The stop member 131 is cylindrical, semi-cylindrical or elongated strip-shaped

[0023] According to another embodiment the rotatable cutting tool 111 comprises a plurality of rotating blades 1111 at a certain vertical distance from each other and a plurality of static blades fixed on the inner wall of the inner set filtering cup 130 (not shown in the FIGURE), wherein each static blade is arranged above or below a corresponding rotating blade 1111, so as to form a cutting stirring knife with a sandwich structure

[0024] In accordance with an embodiment of the present invention, the sieve element of the inner set filtering cup 130 comprises a plurality of filtering holes penetrating through the inner set filtering cup (not shown in the FIGURE), wherein the diameter of the plurality of filtering holes is selected to allow the desired cells to pass therethrough and tissue fragments not decomposed by the digestive enzymes solution being blocked by said diameter. In another embodiment, the sieve element of the inner set filtering cup 130 consists of a plurality of filter holes passing through the bottom of the inner set filtering cup and a screen mesh arranged on the cup bottom (not shown in the FIGURE), wherein the mesh size of the screen mesh is smaller than that of the filter holes of the cup bottom, wherein the required cells can pass the mesh, whereas tissue fragments not being decomposed by the digestive enzyme solution are retained by the mesh.

[0025] In addition, in the present invention, the stirring and shearing member 110 may be separately removable or can be simultaneously removed with the inner set filtering cup 130, wherein the cutting and stirring blades each comprising multiple sandwich structures are designed in a manner, wherein multiple blades are directly stacked in a plurality of layers.

[0026] In the device of the present invention, all static blades (not shown in the FIGURE) respectively arranged on the inner wall of the inner set filtering cup are fixed on the inner wall of the inner set filtering cup by a pin.

[0027] In addition, in the device of the invention, the rotor of the motor can be set to rotate in a forward or backward direction, and the rotating speed thereof being less than 90 rpm.

[0028] According to the invention, the stirring and shearing member 110 has a base 112 connected thereto that is used for supporting the stirring and shearing component 110.