Method of producing and processing diamines to a diamine free base using a carbonate intermediate and an engineered microorganism
11702682 · 2023-07-18
Assignee
Inventors
- Lauri H. Suominen (San Diego, CA, US)
- Connor J. Galleher (San Diego, CA, US)
- Michael Japs (San Diego, CA)
- Mark J. Burk (San Diego, CA, US)
- Cara Tracewell (San Diego, CA, US)
Cpc classification
C12Y402/01001
CHEMISTRY; METALLURGY
C12N1/02
CHEMISTRY; METALLURGY
C12N11/16
CHEMISTRY; METALLURGY
International classification
C12P13/00
CHEMISTRY; METALLURGY
C12N1/02
CHEMISTRY; METALLURGY
C12N11/16
CHEMISTRY; METALLURGY
Abstract
Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
Claims
1. A method for diamine (DA) production comprising the steps of: a) culturing a genetically engineered microorganism in a fermenter in a medium under suitable conditions and for a sufficient period of time to form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, or DA bis-bicarbonate, wherein the DA of the DA carbonate, DA bicarbonate, or DA bis-bicarbonate is selected from the group consisting of hexamethylenediamine, cadaverine, putrescine, ethylenediamine, and heptamethylenediamine, wherein carbon dioxide, carbonate, bicarbonate or carbonic acid predominantly control pH of the medium as a cultured medium, the microorganism comprising at least one exogenous nucleic acid encoding at least one enzyme of a DA synthesis pathway selected from the group consisting of a hexamethylenediamine, a cadaverine, a putrescine, an ethylenediamine, and a heptamethylenediamine synthesis pathway; b) converting the DA carbonate, DA bicarbonate, or DA bis-bicarbonate into DA free base and carbon dioxide; and c) isolating the DA free base.
2. The method of claim 1, wherein the genetically engineered microorganism forms one or more of carbon dioxide, carbonate, bicarbonate, and carbonic acid, the genetically engineered microorganism comprising an exogenous nucleic acid encoding a carbonic anhydrase to form the carbonate, bicarbonate, or carbonic acid.
3. The method of claim 2, wherein the genetically engineered microorganism that forms carbon dioxide, carbonate, bicarbonate, and carbonic acid comprises respiration carbon dioxide or by-product carbon dioxide.
4. The method of claim 3, wherein the respiration carbon dioxide is formed from at least one pathway selected from the group consisting of a TCA cycle, a glyoxylate shunt, a pentose phosphate pathway, and an Entner Duodoroff pathway.
5. The method of claim 3, wherein the by-product carbon dioxide is derived from acetate, ethanol, succinate, 3-oxoadipate, or 3-hydroxyadipate.
6. The method of claim 1, wherein the one or more of carbon dioxide, carbonate, bicarbonate, and carbonic acid is added externally.
7. The method of claim 1, wherein solubility of the cultured medium for carbon dioxide is increased by adjusting back pressure of the fermenter or the temperature.
8. The method of claim 7, wherein the back pressure of the fermenter is adjusted to at least 2 bar but not exceeding 10 bar.
9. The method of claim 7, wherein the temperature is lowered below 37° C.
10. The method of claim 1, wherein the cultured medium has a pH in the range of about 6 to 9.5.
11. The method of claim 1, wherein the cultured medium is substantially free of a buffer, inorganic or organic acid.
12. The method of claim 1, wherein the cultured medium has a total carbonate content and at least 50% of the total carbonate content in the cultured medium comprises the one or more of the DA carbonate, DA bicarbonate, and DA bis-bicarbonate.
13. The method of claim 1, wherein the cultured medium comprises a percent dissolved inorganic carbon (DIC) that is greater than or equal to 40%; wherein the DIC is determined by the formula:
DIC/TDCA×100 where TDCA is the Total Dissolved Counter Anions and is the sum of DIC and other anions.
14. The method of claim 1, wherein at least 40% of diamine species in the cultured medium comprises the one or more of the DA carbonate, DA bicarbonate, and DA bis-bicarbonate.
15. The method of claim 1, wherein the DA synthesis pathway is an HMD synthesis pathway and the DA free base is an HMD free base, optionally the HMD synthesis pathway comprising one or more exogenous nucleic acid(s) encoding an enzyme selected from the group consisting of 3-oxoadipyl-CoA thiolase, 6-ACA transaminase or dehydrogenase, 6-aminocaproyl-CoA reductase, 6-ACA reductase, adipyl-CoA reductase, adipate reductase, 6-hydroxy 3-oxohexanoyl-CoA dehydrogenase, 2-aminopimelate decarboxylase, and homolysine decarboxylase.
16. The method of claim 1, wherein the converting at least one or more of the DA carbonate, DA bicarbonate, and DA bis-bicarbonate into DA free base and carbon dioxide is by heating or sparging with gas.
17. The method of claim 1, further comprising a step of recycling the carbon dioxide to the fermenter after release of carbon dioxide from one or more of the DA carbonate, DA bicarbonate, and DA bis-bicarbonate.
18. The method of claim 1, wherein the step of isolating the DA free base comprises distilling the DA free base from the cultured medium.
19. The method of claim 1, further comprising a step of removing water before the step of isolating the DA free base.
20. The method of claim 1, wherein the genetically engineered microorganism is a bacterial microorganism, optionally E. coli.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
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DETAILED DESCRIPTION
(37) Disclosed is a process for the production and isolation of a diamine. As referred here, “diamines” include C2 to C7 methylene segments such as hexamethylenediamine (HMD), cadaverine, putrescine, ethylenediamine and heptamethylenediamine, for example. The diamines can have carbon content of C2-C7, C3-C7, preferably C4-C7 or even C4-C12 or C2-C12. It should be understood that for ease of reading, HMD may be described in detail but the disclosed process is applicable to any of the diamines such as cadaverine, putrescine, ethylenediamine and heptamethylenediamine.
(38) Hexamethylenediamine also referred to as 1,6-diaminohexane or 1,6-hexanediamine (abbreviated as HMD) has the chemical formula H2N(CH2)6NH2. HMD is an important raw material in the chemical industry. HMD is used, for example, in the preparation of polyamides, polyureas or polyurethanes and copolymers of these materials.
(39) Cadaverine, also referred to as 1,5-diaminopentane is used as a monomer for polyamine production. Engineered microorganisms suitable for fermentative production of cadaverine have been reported. For example, a method to produce and recover a bio-based amine (e.g. cadaverine) is reported in U.S. Pat. No. 8,906,653 and in the literature by Kind et al. “From zero to hero—Production of bio-based nylon from renewable resources using engineered Corynebacterium glutamicum,” (Metabolic Engineering, 25 (2014) pp. 113-123) and Kind et al. “Systems-wide metabolic pathway engineering in Corynebacterium glutamicum for bio-based production of diaminopentane,” (Metabolic Engineering 12 (2010) 341-351). These reported processes involve active neutralization of a fermentation broth or cultured medium with an inorganic acid (e.g. sulfuric acid). After fermentation, the cultured medium or broth is alkalized with a strong base to deprotonate the amine, which is then extracted with an organic solvent and subsequently distilled. In these processes, copious amounts of unwanted salt by-products are produced with the amine.
(40) In another method, lysine carbonate prepared in vitro is enzymatically decarboxylated to produce cadaverine carbonate with addition of a dicarboxylic acid salt to maintain suitable pH for the decarboxylation reaction, followed by concentration to generate cadaverine and cadaverine-dicarboxylic acid salt, as reported in International Patent Application Publication No. WO 2006/123778. In still another method, lysine carbonate prepared in vitro is enzymatically decarboxylated to produce cadaverine carbonate which is thermally treated and then distilled to provide cadaverine as reported in International Patent Application Publication No. WO 2010/002000.
(41) Putrescine, also referred to as 1,4-diaminobutane is used as a monomer for polyamine production. Engineered microorganisms suitable for fermentative production of putrescine have been reported. See, for example, Schneider et al. “Improving putrescine production by Corynebacterium glutamicum by fine-tuning ornithine transcarbamoylase activity using a plasmid addition system,” (Appl Microbiol Biotechnol. 2012; 95(1):169-78); and U.S. Patent Application Publication No. 20140004577A1 “Microorganisms for producing putrescine and method for producing putrescine using same.”
(42) Heptamethylenediamine, also referred to as 1,7-diaminoheptane, is used as a monomer for polyamine production. Engineered microorganisms suitable for fermentative production of putrescine have been reported. See, for example International Patent Application Publication No. WO2014105790A2, “Methods of producing 7-carbon chemicals via c1 carbon chain elongation associated with coenzyme b synthesis.”
(43) Ethylenediamine is used as a monomer for polyamine production as well as a precursor to other chemicals. Engineered microorganisms for fermentative production of ethylenediamine have been reported. See for example International Patent Application Publication No. WO2014049382A2, “Ethylenediamine fermentative production by a recombinant microorganism.”
(44) Hexamethylenediamine also referred to as 1,6-diaminohexane or 1,6-hexanediamine (abbreviated as HMD) has the chemical formula H.sub.2N(CH.sub.2).sub.6NH.sub.2. HMD is an important raw material in the chemical industry. HMD is used, for example, in the preparation of polyamides, polyureas or polyurethanes and copolymers of these materials. Cadaverine, also referred to as 1,5-diaminopentane is used as a monomer for polyamine production. Putrescine, also referred to as 1,4-diaminobutane is used as a monomer for polyamine production. Heptamethylenediamine, also referred to as 1,7-diaminoheptane, is used as a monomer for polyamine production. Ethylenediamine is used as a monomer for polyamine production as well as a precursor to other chemicals. Engineered microorganisms for fermentative production of these compounds and other diamines or their immediate precursors have been reported. Typically, processes for their fermentation and isolation require acids and bases that generate salt by-products.
(45) During the fermentation process, which utilizes carbon dioxide, at least one or more of diamine carbonate, diamine bicarbonate, and/or diamine bis-bicarbonate (referred to herein as the diamine “Carbonates”) and, optionally diamine carbamate or diamine biscarbamate (referred to herein as the diamine “Carbamates”, are produced. The disclosed process further provides increased yields of the diamines and improves the purification of the desired diamine using organic solvent-based extraction.
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(49) Culturing a microorganism in medium under suitable conditions and for a sufficient period of time results in the formation of one or more diamine Carbonates and/or Carbamates. The produced compounds in the cultured medium include at least 40% Carbonates and/or Carbamates in the cultured medium. In other embodiments, the Carbonates and/or Carbamates can be at least 50%, 60%, 70%, 80, 90% or 99.9% in the cultured medium. As defined above, this means that desired diamine (e.g. HMD) carbonates or carbamates comprise at least 40% or more of all carbonates and/or carbamates in the cultured medium.
(50) Culture Medium
(51) Depending on the desired microorganism or strain to be used, the appropriate culture medium may be used. For example, descriptions of various culture media may be found in “Manual of Methods for General Bacteriology” of the American Society for Bacteriology (Washington D.C., USA, 1981). As used here, “medium” as it relates to the growth source refers to the starting medium be it in a solid or liquid form. “Cultured medium”, on the other hand and as used here refers to medium (e.g. liquid medium) containing microbes that have been fermentatively grown and can include other cellular biomass. The medium generally includes one or more carbon sources, nitrogen sources, inorganic salts, vitamins and/or trace elements.
(52) Exemplary carbon sources include sugar carbons such as sucrose, glucose, galactose, fructose, mannose, isomaltose, xylose, pannose, maltose, arabinose, cellobiose and 3-, 4-, or 5-oligomers thereof. Other carbon sources include alcohol carbon sources such as methanol, ethanol, glycerol, formate and fatty acids. Still other carbon sources include carbon sources from gas such as synthesis gas, waste gas, methane, CO, CO.sub.2 and any mixture of CO, CO.sub.2 with H.sub.2. Other carbon sources can include renewal feedstocks and biomass. Exemplary renewal feedstocks include cellulosic biomass, hemicellulosic biomass and lignin feedstocks.
(53) In some embodiments, culture conditions include anaerobic or substantially anaerobic growth or maintenance conditions. Exemplary anaerobic conditions have been described previously and are well known in the art. Exemplary anaerobic conditions for fermentation processes are disclosed, for example, in U.S. Patent Application Publication No 2009/0047719, filed Aug. 10, 2007. Any of these conditions can be employed with the microbial organisms as well as other anaerobic conditions well known in the art.
(54) The culture conditions can include, for example, liquid culture procedures as well as fermentation and other large scale culture procedures. As described herein, particularly useful yields of the products can be obtained under anaerobic or substantially anaerobic culture conditions.
(55) An exemplary growth condition for achieving, hexamethylenediamine includes anaerobic culture or fermentation conditions. In certain embodiments, the microbial organism can be sustained, cultured or fermented under anaerobic or substantially anaerobic conditions. Briefly, anaerobic conditions refer to an environment devoid of oxygen. Substantially anaerobic conditions include, for example, a culture, batch fermentation or continuous fermentation such that the dissolved oxygen concentration in the medium remains between 0 and 10% of saturation. Substantially anaerobic conditions also includes growing or resting cells in liquid medium or on solid agar inside a sealed chamber maintained with an atmosphere of less than 1% oxygen. The percent of oxygen can be maintained by, for example, sparging the culture with an N2/CO2 mixture or other suitable non-oxygen gas or gases.
(56) The culture conditions can be scaled up and grown continuously for manufacturing hexamethylenediamine. Exemplary growth procedures include, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation. All of these processes are well known in the art. Fermentation procedures are particularly useful for the biosynthetic production of commercial quantities of, hexamethylenediamine. Generally, and as with non-continuous culture procedures, the continuous and/or near-continuous production of hexamethylenediamine will include culturing a hexamethylenediamine producing organism on sufficient nutrients and medium to sustain and/or nearly sustain growth in an exponential phase. Continuous culture under such conditions can include, for example, 1 day, 2, 3, 4, 5, 6 or 7 days or more. Additionally, continuous culture can include 1 week, 2, 3, 4 or 5 or more weeks and up to several months. Alternatively, the desired microorganism can be cultured for hours, if suitable for a particular application. It is to be understood that the continuous and/or near-continuous culture conditions also can include all time intervals in between these exemplary periods. It is further understood that the time of culturing the microbial organism is for a sufficient period of time to produce a sufficient amount of product for a desired purpose.
(57) Fermentation procedures are well known in the art. Briefly, fermentation for the biosynthetic production of hexamethylenediamine can be utilized in, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation. Examples of batch and continuous fermentation procedures are well known in the art.
(58) The culture medium at the start of fermentation may have a pH of about 5 to about 7. The pH may be less than 11, less than 10, less than 9, or less than 8. In other embodiments the pH may be at least 2, at least 3, at least 4, at least 5, at least 6, or at least 7. In other embodiments, the pH of the medium may be about 6 to about 9.5; 6 to about 9, about 6 to 8 or about 8 to 9.
(59) CO2 Sources and Species
(60) As noted above, to produce the desired diamine (e.g. HMD) and to control pH of the culture medium CO2 is added. The source of CO2 can take the form of CO2, carbonate, bicarbonate or carbonic acid, for example. In one embodiment, CO2 may be externally added to the cultured medium. In other embodiments, CO2 may be produced by the microorganism such as by respiration or as a by-product. For example, the respiration CO2 may be formed from the conversion of the tricarboxylic acid (TCA) cycle, via the glyoxylate shunt, the pentose phosphate pathway (e.g. gnd (6-phosphogluconate dehydrogenase that converts 6-phosphogluconate to ribuloase-5-phosphate and CO.sub.2)) or the Entner Duodoroff pathways. In other embodiments, the by-product CO2 may be formed from acetate, ethanol, succinate, 3-oxoadipate or 3-hydroxyadipate.
(61) Culturing the microorganism under suitable conditions and sufficient periods of time also results in the microorganism forming one or more CO2 sources that include CO2, carbonate, bicarbonate or carbonic acids. In one embodiment, the microorganism forms CO2 stoichiometrically with the diamine.
(62) Stoichiometric CO2 as referred here is the amount of CO2 associated with the formation of the product from a given substrate based on the stoichiometry. One exemplary stoichiometry for the production of HMD from glucose is:
1.47C6H12O6+0.321O2+2NH3.fwdarw.C6H16N2+2.821CO2+3.821H2O
The stoichiometric CO2 amount produced per mole of HMD is 2.821 moles. Any CO2 produced higher than this amount is either produced by respiration or byproduct formation.
(63) Some sources of respiratory CO2 are the TCA cycle, glyoxylate shunt, pentose phosphate pathway (e.g. zwf) and the Entner-Duodoroff pathway among others. These pathways produce NAD(P)H that can then be used via the electron transport chain to form ATP. Byproduct CO2 is defined as the CO2 produced due to the formation of byproducts. For example, 2 moles of acetate are produced from every mole of glucose and this is associated with the release of 2 moles of CO2. Therefore, the byproduct CO2 associated with the formation of each mole of acetate is 1 mole.
(64) In another example, the production of HMD from methanol can have multiple stoichiometries, depending upon whether only the oxidative TCA branch is used to make HMD, compared if both the oxidative and the reductive TCA branches are used to make HMD
6.31CH.sub.3OH+2NH.sub.3+0.97O.sub.2+C.sub.6H.sub.16N.sub.2+7.62H.sub.2O+0.31CO.sub.2
.sub.7CH.sub.3OH+2NH.sub.3+2O.sub.2+C.sub.6H.sub.16N.sub.2+9H.sub.2O+CO.sub.2
(65) In the case where CO2 is used to drop the pH (e.g. to 7), the solubility of the medium towards CO2 may be enhanced by raising the back pressure at the top of the fermentor to at least 2, but not exceeding 10 bar to increase the solubility of carbon dioxide. In other embodiments, the temperature may be lowered to enhance solubility of the medium for CO2. In one embodiment, the temperature is lowered below 37° C. to increase the solubility of CO2.
(66) In one embodiment, a microorganism producing the diamine is cultured in a liquid medium in a fermenter, wherein an inlet gas including carbon dioxide is fed into the fermenter and the back pressure at the top of the fermentor is raised to at least 2, but not exceeding 10 bar to increase the solubility of carbon dioxide.
(67) In another embodiment, the process for the fermentative production of a diamine, wherein a microorganism producing the diamine is cultured in a liquid medium in a fermenter, wherein an inlet gas including carbon dioxide is fed into the fermenter and the temperature is lowered below 37° C. to increase the solubility of CO2.
(68) In some embodiments, an enzyme carbonic anhydrase (CA) may be added to the fermentation broth or medium to catalyze or enhance the formation of diamine Carbonates and/or diamine Carbamates (e.g. HMDA Carbonates) by increasing the amount or rate of gaseous CO2 converted to soluble ion, thus a greater amount or availability of soluble ion is available to the diamine or HMD.
(69) Carbonic anhydrase catalyzes a reversible reaction. In the forward reaction, CA combines carbon dioxide with water:
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and H2CO3 dissociates to form bicarbonate (HCO3−) and a proton. In the reverse, reaction, CA combines bicarbonate and a proton to provide carbon dioxide and water. Therefore, the reversible reaction, in particular the reverse reaction can be used to strip or release the CO2 after the DA carbonate is formed. In some embodiments, the CA may be used to hydrate CO2 in the form of bicarbonate and proton, which in turn may be converted to a DA. Depending on the direction of the reaction, certain suitable conditions can be selected that favor the absorption of carbon dioxide into a solution (e.g., via hydration of carbon dioxide to bicarbonate) and/or the desorption of carbon dioxide from a solution (e.g., via dehydration of bicarbonate to carbon dioxide and water).
(71) The carbonic anhydrase may be provided exogenously by directly providing the CA to the fermentation solution or may be introduced via a microorganism capable of producing carbonic anhydrase. Carbonic anhydrase may also be provided as a recombinant or engineered CA. The recombinant CA may be part of a microorganism that includes a diamine pathway (e.g. HMD synthesis pathway) or may be introduced by another microorganism capable of CA expression. The microorganism's native CA may be used, for example by overexpressing it or engineering it to be excreted into the fermentation broth or solution or be secreted into the microorganism's periplasm. The CA may be of the EC 4.2.1.1. enzyme class. The CA may be that of an Escherichia, for example Can gene or b1026 (KEGG designation) or other host strain, including strains listed herein. In some embodiment sthe CA may be obtained from the genus Methanobacterium, Desulfovirbio, Methanosarcina, Thiomicrospira, Acetobacterium, Clostridium, Methylobacterium, Rhizobium, Rhodobacter, Rhodospirillum, Staphylococcus, Methanococcus, Methanosaeta, Methanospirillum, Sulfolobus. (Smith et al., 1999 PNAS 96(26):15184-15189.
(72) Exemplary organisms from which the CA may be obtained include Neisseria gonorrhoeae (Jo et al., 2013 Appl. Environ. Microbiol. 79(21):6697-6705), Methanosarcina, Thiomicrospira, Acetobacterium woodii, Clostridium thermoaceticum, Methylobacterium extorquens, Rhizobium meliloti, Rhodobacter capsulatus, Rhodobacter sphaeroides, Rhodospirillum rubrum, Staphylococcus aureus, Methanococcus jannaschii, Methanosaeta concilii, Methanosarcina barkeri, Methanosarcina thermophila, Methanospirillum hungateii, Sulfolobus solfataricus. (Smith et al., 1999 PNAS 96(26):15184-15189. The CA may be obtained from Neisseria gonorrhoeae (Jo et al., 2013 Appl. Environ. Microbiol. 79(21):6697-6705). In some embodiments, a carbonic anhydrase of a Neisseria gonorrhoeae may be engineered for periplasmic expression in E. coli as reported Jo et al., 2013 Appl. Environ. Microbiol. 79(21):6697-6705
(73) In some embodiments, the gene encoding a β type CA may be obtained from the genus Desulfovirbio such as Desulfovirbio fructosivorans, Desulfovirbio Tom C, Desulfovirbio magneticus, Desulfovirbio alcholivorans. In still other embodiments, the CA may be obtained from the genus Desulfomonile such as Desulfomonile tiedjei, Methanobacterum such as Methanobacterium thermoautotrophicum, Metanoacina such as Metanoacina thermophilia, Thiomicrospira such as Thiomicrospira crunogena.
(74) In other embodiments, the CA may be used as shown in Table A below:
(75) TABLE-US-00002 TABLE A % Sequence Identifiers Identity Sequence gi| 506426310|ref| 100 [Desulfovibrio vulgaris str. WP_015946029.1|; ‘Miyazaki F’] gi|218758438|gb|AC MRLRFLSALFLWAMVGTALAGSTGPGIGPDEALQRLK L09337.11 EGNARFVAETPTRQNLSAKRLATSQHGQTPYATILSC ADSRAPVELIFDEGVGDLFVIRVAGNVAATDEVGTAE YGADHLNVPLLWMGHTQCGAVTAWQGAEVHGSIPMLV APIVPAVTAVEKSNPKHDRAALVPKVIEANVWQAIDD TMRQSPIIRARVAAGKLKWGAIYHIDDGKVEWLGEHP MQARLLNYTSGPAKAHR gi|496399538|ref| 55.1 [Desulfovibrio sp. U5L] WP_009108528.1|; MKRFLAATATMAFLLAMCTAVLASSGGPEVSADEALS gi|385733492|gb|EIG RLKEGNTRFVSQANVAPHQDAARRHETATGGQHPFAT 53690 11 VLSCADSRAPVEVLFDQGVGDLFVVRVAGNVAATDEI GTIEYGAEHLGVPLVWLAHTKCGAVTAVVKNEPVTEN IGKLVAPIVPAVKGVKARFAASDVNEIISRSIEANMW QAVSDIYAKSPMLKKMAADGKIKWGALYDIDSGEVHW FGEHPSEGNLLDN gi|760112986|ref| 59.9 [Desulfovibrio fructosivorans] WP_043794941.11 MKRAFAAFAAAVFVAATCALALASSAGPGLTSDEALA KLKEGNDRYVAKASVAPRRDAARRHETATGGQHPFAT VLACSDSRVPVEWFDQGVGDIFVVRVAGNVAATDEIG TMEYGAEHLGVPLIWMGHTKCGAVSAVVKNEPVTENI GKLVAPIVPAVKSVKARFATANTDELIAKSIEANVWQ AISDIYAKSPLIKKMAAAGKVKVVGALYDIDSGEVHW LGEHPNNAILLGK gi|302490589|gb| 59.9 [Desulfovibrio fructosivorans JJ] EFL50494.1| MMKRAFAAFAAAVFVAATCALALASSAGPGLTSDEALAK LKEGNDRYVAKASVAPRRDAARRHETATGGQHPFATVLA CSDSRVPVEWFDQGVGDIFWRVAGNVAATDEIGTMEYGA EHLGVPLIWMGHTKCGAVSAWKNEPVTENIGKLVAPIVP AVKSVKARFATANTDELIAKSIEANVWQAISDIYAKSPL IKKMAAAGKVKVVGALYDIDSGEVHWLGEHPNNAILLGK gi|759946892|ref| 58.4 [Desulfovibrio sp. TomC] WP_043631526.1| MRRNMTAMTVVIWTLCMATTALAFSGGAGITADEALSRL KEGNTRFVAGAAVTPRQDAARRHETTVGGQHPFATVLAC ADSRVPVEAIVDQGVGDVFWRVAGNVANTDEIGTIEYGA EHLGVPLVWLGHTKCGAVTAWKGEHVTENIGKLVAPIVP AVAGVKNRFASADLDELINRSIEANVWQSISDMYANSPL LKKMAADGKLKWGALYDIDSGDIHWLGEHPSNAKLLGN gi|732991830|gb| 58.3 [Desulfovibrio sp. TomC] KH K04204.1| MTVVIWTLCMATTALAFSGGAGITADEALSRLKEGNTRF VAGAAVTPRQDAARRHETTVGGQHPFATVLACADSRVPV EAIVDQGVGDVFWRVAGNVANTDEIGTIEYGAEHLGVPL VWLGHTKCGAVTAWKGEHVTENIGKLVAPIVPAVAGVKN RFASADLDELINRSIEANVWQSISDMYANSPLLKKMAAD GKLKVVGALYDIDSGDIHWLGEHPSNAKLLGN gi|493978453|ref| 55.7 [Desulfovibrio magneticus] WP_006921438.1|; MKRFVTAFAGAVITISMAGAAMAFSGGAGISADEALARL gi|409981683|gb| KEGNTRYVAGAAVTPRQDAARRHETATGGQHPFVSVLSC EKO38218.11 ADSRVPVETVFDQGIGDVFVIRVAGNVANTDEIGTIEYG AEHLGTPLVLVMAHTKCGAVTAVVKGEHVTENIGKLVAP IVPAVASVKSRFATDDVNELINRSIEANMWQAIADMYAK SPLLKKMAADGKIKWGALYDIDSGEVHWFGEHPSNANLL GK gi|496471458|ref| 54.7 [Desulfovibrio sp. FW1012B] WP_009180303.1|; MKRFLAATATMAFLLAMCTAVLASSGGSEVSADEALSR gi|357581548|gb| LKEGNTRFVSQANVAPHQDAARRHETATGGQHPFATVL EHJ46881.1| SCADSRAPVEVLFDQGVGDLFVVRVAGNVAATDEIGTI EYGAEHLGVPLVWLAHTKCGAVTAVVKNEPVTENIGKL VAPIVPAVKGIKARFAASDVNEIISRSIEANMWQAISD IYAKSPMLKKMAADGKIKVVGALYDIDSGEVRWFGEHP SEGSLLDN gi|752616536|ref| 53.8 [Desulfomonile tiedjei] WP_041285901.1| MEAFMKKIAVLFSVICMLGSVFSWAADPAATVSPEEAV KLLKEGNGRFIAGTSQHPNNDLQRRNTTAAQGQHPFVT VLSCSDSRVPVEVLFDRGVGDIFVIRVAGNVANGDEVG SIEYAVDHLGTPLLVILGHTKCGAVTAVVQSAELLGNI IPIGKSIFPAVVAAKKSNPKASGDALINDAIKANWVQA IEDIYRTSPITAARVKSGKLKVVGALYDIESGNVSWLG SHPKEGGLLSDKGH gi|390622030|gb| 53.8 [Desulfomonile tiedjei DSM 6799] AFM23237.1| MKKIAVLFSVICMLGSVFSWAADPAATVSPEEAVKLLK EGNGRFIAGTSQHPNNDLQRRNTTAAQGQHPFVTVLSC SDSRVPVEVLFDRGVGDIFVIRVAGNVANGDEVGSIEY AVDHLGTPLLVILGHTKCGAVTAWQSAELLGNIIPIGK SIFPAWAAKKSNPKASGDALINDAIKANVWQAIEDIYR TSPITAARVKSGKLKWGALYDIESGNVSWLGSHPKEGG LLSDKGH gi|657653962|ref| 58.8 [Desulfovibrio alcoholivorans] WP_029458402.1| MKRLFTATTMLALLLACCALALASSGGPGLTAD EALAKLKEGNMRYVAQASVAPHQDAARRHETAT DGQHPFATILSCADSRVPLEIIFDQGVGDIFAV RVAGNVAAVDEIGTMEYGAEHLGVPLIVVLGHT KCGAVTAVVKNEPVTENIGQLVAPIVPAVKSVK SRFASASLDELINKSIEANVWQAVSDIYAKSPL LKKMAAAGKVKWGALYDIDSGKVQWFGEHPSNA SLLGK gi|5063410771ref| 54.9 [Desulfovibrio magneticus] WP_015860796.1|; MKRFVAAFAGAVITFSMAGAAMAFSGGAGISAD gi|239796622|dbj| EALARLKEGNTRYVAGAAVTPRQDAARRHETAT BAH75611.1| GGQHPFVSVLSCADSRVPVETVFDQGIGDVFVI RVAGNVANTDEIGTIEYGTEHLGTPLVWLAHTK CGAVTAVVKGEHVTENIGKLVAPIVPAVASVKS RFASGDLNELINRSIEANMWQAIADMYAKSPLL KKMAADGKIKVVGALYDIDSGDVHWFGEHPSNA NLIGK Escherichia coli str. Escherichia coli str. K-12 substr. K-12 substr. (MG1655] MG1655]yadF MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQK PRFLWIGCSDSRVPAERLTGLEPGELFVHRNVAN LVIHTDLNCLSWQYAVDVLEVEHIIICGHYGCGG VQAAVENPELGLINNWLLHIRDIWFKHSSLLGEM PQERRLDTLCELNVMEQVYNLGHSTIMQSAWKRG QKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRY RHGISNLKLKHANHK >b0339; NP_414873.1; Escherichia coli GI: 16128324]CynT MKEIIDGFLK FQREAFPKRE ALFKQLATQQ SPRTLFISCS DSRLVPELVT QREPGDLFVI RNAGNIVPSY GPEPGGVSAS VEYAVAALRV SDIVICGHSN CGAMTAIASC QCMDHMPAVS HWLRYADSAR VVNEARPHSD LPSKAAAMVR ENVIAQLANL QTHPSVRLAL EEGRIALHGW VYDIESGSIA AFDGATRQFV PLAANPRVCA IPLRQPTAA GI: 157878699 HTHWGYTGHD SPESWGNLSE EFRLCSTGKN QSPVNITETV SGKLPAIKVN YKPSMVDVEN NGHTIQVNYP EGGNTLTVNG RTYTLKQFHF HVPSENQIKG RTFPMEAHFV HLDENKQPLV LAVLYEAGKT NGRLSSIWNV MPMTAGKVKL NQPFDASTLL PKRLKYYRFA GSLTTPPCTE GVSWLVLKTY DHIDQAQAEK FTRAVGSENN RPVQPLNARV VIE
(76) The CA may be encoded by Desulfovibrio vulgaris. Desulfovibrio vulgaris which has unique properties of having high activity in 4.2 M N-methyldiethanolamine (MDEA) at elevated temperatures and pH>10. The D. vulgaris CA has been evolved to be active at 100° C. for long periods of time (8 weeks) in high concentrations of MDEA for use in carbon capture technology (Alvizo, et. al. 2014 PNAS 111(46): 16436-16441). In some embodiments, the engineered CA is from Desulfovibrio vulgaris (GenBank accession ACL09337.1 GI: 218758438). In some embodiments, the Desulfovibrio vulgaris str “Miyazaki F” carbonic anhydrase has amino acid substitution to stabilize the carbonic anhydrase activity at elevated temperatures and alkaline pH by including one or more substitutions identified as: A56S, T30R, A40L, A84Q, G120R, T139M, K37R, E68AQ, A95V, Q119M, N145WFC, N213E, A219T, R31P, Q43M, V701, H124T, H148T, V157A, M170F, H44L, M129F, S144R, Y49F, S126N, D196S, P136R, P174E, D195A, G89A, D96E, V100T, A121Q, A181K, M207A, S216D.
(77) In some embodiments, a CA is encoded by E. coli (EG10176 (EcoCyc), or EG12319 (EcoCyc), Can gene, b1026 (KEGG designation)).
(78) The disclosed enzymes may also be in the form of fusion proteins in which the recombinant or engineered CA are fused to antibody tags (e.g. myc epitope), purification sequences (e.g. His tags for binding to metals and cell localization signals (e.g. secretion or excretion signal). To aid in the expression of the desired protein into periplasmic space, a secretion signal may be use such as a Sec tag or Tat tag. In preferred embodiments the CA is excreted into the media rather than secreted to the periplasmic space. In some embodiments, the secretion or excretion signal is fused to the N-terminus of the protein by genetically encoding the secretion tag as a fusion to the carbonic anhydrase DNA sequence to aid expression into the periplasmic space or extracellularly excreted from the microorganism (e.g. E. coli). In another embodiment, CA may be fused to an E. coli protein OmpF that is transported (excreted) to the culture medium (Nagahari et al., 1985 The EMBO J. 4(13A):3589-3592; Jeong and Lee, 2002 Appl. Environ. Microbiol. 68:4979-4985). In still another embodiment CA may be fused to the E. coli protein YebF which as been shown to support protein export to the culture medium, which has an unknown function, but is an extracellular protein (Zhang et al., 2006 Nat. Biotech. 24:100-10). An N-terminal secretion signal peptide tag is identified using SignalP 4.1 Server (http://www.cbs.dtu.dk/services/SignalP/).
(79) The carbonic anhydrase may be provided by a genetically engineered microorganism in the fermentation broth. In other embodiments, the carbonic anhydrase is provided by a genetically engineered microorganism that produces the DA, optionally excreted to the broth, and optionally present in the microorgansim's periplasm. In other embodiments, the carbonic anhydrase is a native gene or enzyme, optionally engineered for secretion to the broth, and optionally engineered for secretion to the microrganism's periplasmic space.
(80) In some embodiments, the CA may be excreted into the fermentation broth, and in other embodiments, the CA may be present in the microorgansim's periplasm.
(81) Depending on the direction of the reaction or the speed of the reaction, in some embodiments the native CA gene (e.g. E. coli coded CA) sequence may be overexpressed, for example by modifying its promoter, and or engineered with a secretion or excretion peptide or fusion.
(82) In some embodiments, variants of the CA that are capable of carrying out the forward and/or reverse reactions are contemplated. The variant can be a homolog, ortholog, paralogs or genetically engineered, for example increased alkaline pH and heat stability.
(83) CA having an improved property (e.g., thermal stability, solvent stability, and/or base stability) that allows them to enhance the forward and/or reverse reaction may be selected for and used. In some embodiments, CA variants that are active and/or stable at high concentration of DA (e.g. HMD) may be selected for and used. In other embodiments, CA and variants thereof that are active and/or stable at high concentrations of C2 to C7 methylene segments such as hexamethylenediamine (HMD), cadaverine, putrescine, ethylenediamine and heptamethylenediamine may selected and used.
(84) Depending on the conditions selected, the CA may be a thermostable CA or may be a alkaline pH stable CA, or both. Preferably the alkaline pH is about pH 8-13. In some embodiments, the pH may range from pH 8-13, pH 8.5 to 13, pH 9-13, pH 10-13, pH 8-12, pH 8.5-12, pH 9-12, pH 8-11, pH 8.5-11, pH 9-11, pH 10-11 and pH 10-12. Temperature and basic pH stability can be useful if the CA is used in the step or steps for release of CO2 from the DA Carbonate or Carbamate. This step can result in increase in pH as CO2 is released and free base formed. Additionally in some embodiments temperatures above room temperature and above typical fermentation temperatures may be used to facilitate release of CO2.
(85) Depending on whether the forward reaction or the reverse reaction is favored, the fermentation broth may be provided with CA enzymes that have different activity. In some embodiments, the CA may have optimal activity for the forward reaction and in other embodiments the CA enzyme may have optimal activity for the reverse reaction. In some embodiments, a mixture of different enzymes having varying optimal activities and/or improved properties as disclosed herein may be used.
(86) In some embodiments, the method can be carried out wherein the carbonic anhydrase has the improved property at least 1.2-fold, at least 1.3-fold, at least 1.5-fold, at least 2-fold, at least 3-fold, at least 4-fold, at least 5-fold, at least 10-fold, or at least 25-fold increased activity of hydrating carbon dioxide or dehydrating bicarbonate under suitable conditions. Accordingly, in some embodiments, the suitable conditions used in the method can comprise a concentration of the carbonic anhydrase polypeptide of from about 0.1 to about 10 g/L, about 0.25 g/L to about 7.5 g/L, about 0.5 g/L to about 5 g/L, less than 10 g/L, less than about 5 g/L, or less than about 2.5 g/L.
(87) In some embodiments, the CA may be provided exogenously. In other embodiments, the CA enzyme may be provided directly into the broth or fermentation solution. In some embodiments, the CA is provided by a genetically engineered microorganism in the fermentation broth or solution, where the CA can be excreted into the broth or may be present in the microorganism's periplasm. In other embodiments, the enzyme may be provided immobilized on particles. Recombinant carbonic anhydrase polypeptide may be immobilized on a surface, for example wherein the enzyme is linked to the surface of a solid-phase particle in the solution. Methods for linking (covalently or non-covalently) enzymes to solid-phase particles (e.g., porous or non-porous beads, or solid supports) such that they retain activity for use in bioreactors are well-known in the art. Methods for treating a gas stream using immobilized enzymes are described in e.g., U.S. Pat. No. 6,143,556, U.S. patent publication no. 2007/0004023 A1, and PCT publications WO98/55210A1, WO2004/056455A1, and WO2004/028667A1, each of which is hereby incorporated by reference herein.
(88) Accordingly, in some embodiments, the methods for enhancing the release of CO2 from a solution of DA Carbonates or DA Carbamates can be carried out where the engineered carbonic anhydrase polypeptide is immobilized on a surface, for example where the enzyme is linked to the surface of a solid-phase particle (e.g., beads). In some embodiments, the methods using immobilized polypeptides can be carried out where the method further includes a step of isolating or separating the immobilized carbonic anhydrase from the broth or fermentation solution. After separating the immobilized carbonic anhydrase from the broth or fermentation solution, the broth or fermentation solution can be treated to conditions that may inactivate the enzyme, e.g., desorption of CO2 at high temperatures. Further, the separately retained immobilized enzyme can be added to another solution and reused. CO2:DA Ratios
(89) Under the disclosed process, the microorganism (e.g. genetically engineered microorganism) forms CO2 and DA (e.g. HMD) in the ratio of about 0.05 to 1 to about 5 to 1 to about 7:1. Other suitable ratios include 0.2 to 1 to about 3 to 1. In other embodiments, the ratio of CO2 to DA (e.g. HMD) include about 0.05 to 1 to about 3 to 1; about 0.05 to 1 to about 2.5 to 1; about 0.05 to 1 to about 2 to 1; about 0.05 to 1 to about 1.5 to 1; about 0.05 to 1 to about 1 to 1.
(90) The disclosed ratios may be determined by measuring the CO2 in the form of the total dissolved inorganic carbon (DIC) in the cultured medium. The DIC, which include the Carbonates and/or Carbamates may be measured, for example by the “Handbook of Methods for the Analysis of the Various Parameters of the Carbon Dioxide System in Sea Water.” Prepared for the U. S. Department of Energy, Special Research Grant Program 89-7A: Global survey of carbon dioxide in the oceans. Version 2—September 1994 Edited by Andrew G. Dickson & Catherine Goyet (referred to as the Handbook). For example, the DIC may be measured by the “SOP 2: Determination of total dissolved inorganic carbon in sea water, p. 1-18” on pages 38-55 of the Handbook.
(91) In other embodiments, the fraction of DIC, which is DIC over Total Dissolved Counter Anions (TDCA) may be measured. The TDCA is the sum of DIC and other anions. The other anions (e.g., Cl.sup.−, SO.sup.−2, PO.sub.4.sup.−3, NO.sub.3.sup.−, NO.sub.2.sup.−) other than the DIC can be determined using any suitable method such as ion exchange chromatography. For example, a commercially available ion exchange chromatography by DIONEX with conductivity detector (and ion suppressor) may be used.
(92) The produced compounds in the cultured medium include have a DIC/TDCA value of at least 40%. In other embodiments, the DIC percentage can be at least 50%, 60%, 70%, 80, 90% or 99.9% in the cultured medium. In some embodiments, the DIC is at least 40%, 50%, 60%, 70%, 80, 90% or 99.9% of TDCA in the cultured medium at pH 9.
(93) Fermentation pH
(94) As noted above, the starting culture medium may have a pH of about 5 to about 7. As the microorganism grows on the culture medium and produces the desired diamine (e.g. HMD-Carbonates and/or HMD Carbamates) and before the diamine Carbonates and/or Carbamates are converted to the diamine free base, the pH of the cultured medium may be less than 11, less than 10, less than 9, or less than 8. In other embodiments the pH may be at least 2, at least 3, at least 4, at least 5, at least 6, or at least 7. In other embodiments, the pH of the medium may be about 6 to about 9.5; about 6 to about 9, about 6 to about 8, about 6.5 to about 7.5, about 7.5 to about 9.5 or about 8 to about 9.
(95) While the medium (i.e. the starting medium) may be adjusted with inorganic acids, bases or buffers to adjust pH, the cultured medium is substantially free of buffer, substantially free of inorganic or organic acid, or externally added inorganic or organic acid. As used here, “substantially free” relates to the cultured medium. In other words, salts, buffers, acids (not including CO2) or bases may be used to control pH of the starting medium. If such salts, buffers, acids (not including CO2) or bases are used to adjust pH fluctuations as fermentation and growth of the organism progresses, minimal amounts are used. But the salts, buffers, acids (not including CO2) or bases are not used to neutralize the diamine Carbonate and/or Carbamate. It should be understood the microorganism will produce by-products such as acetates, succinates, other salts and/or organic acids. Adjusting pH during fermentation is by the use of carbon dioxide that may be added externally or generated by the microorganism's growth.
(96) Release of CO2 & Diamine Free Base
(97) Once the diamine-Carbonates and/or Carbamates are formed, the diamine is obtained by converting to diamine free base, e.g. HMD free base. In some embodiments, the DA-Carbonates and/or Carbamates are first separated from the microorganism in the cultured medium before converting to DA free base. Examples of converting DA-Carbonates and/or Carbamates to DA free base include by heat, vacuum, ion exchange or electrodialysis. In one embodiment, the diamine is an HMD free base that may be converted by releasing carbon dioxide. In some embodiments, a carbonic anhydrase enzyme (as described more fully above in the context of the forward reaction) may be provided to enhance the release of carbon dioxide from a solution of DA Carbonates or DA Carbamates by converting a bicarbonate and/or carbonate ions to carbon dioxide. In such embodiments, the enzymes may be provided exogenously, for example, as an engineered enzyme that may be part of a microorganism or exogenously added. In some embodiments, the engineered CA may be engineered to be excreted into the fermentation broth or fermentation solution. In other embodiments, the engineered CA may be engineered to be in the microorganism's periplasm. In some embodiments, the DA-Carbonates and/or Carbamates are first separated from the microorganism in the cultured medium before converting to DA free base. In such embodiments, the CA may be provided by exogenously adding the CA. In other embodiments, the CA may be immobilized. In other embodiments, the CA is part of an engineered microorganism capable of providing carbonic anhydrase activity. In other embodiments, the carbonic anhydrase activity is provided by an engineered microorganism that includes a DA synthesis pathway such as a HMD synthesis pathway and the carbonic anhydrase activity.
(98) If heat is used to convert diamine Carbonates and/or Carbamates (e.g. HMD) to diamine free base, the temperatures include greater than 70° C., greater than 80° C. or greater than 105° C. In some embodiments, the temperature may be greater than 200° C. In still other embodiments, the temperature may be about 315° C. In some embodiments, the temperature is less than 315° C., less than 250° C. or less than 215° C. In still other embodiments, the temperature may be greater than 20° C., greater than 30° C., or greater than 40° C. and where a vacuum is used. In some embodiments, the diamine Carbonates and/or Carbamates converted to free base at temperatures disclosed above may be HMD. CA may also be added to the heating step to enhance the release of carbon dioxide from a solution of DA Carbonates or DA Carbamates by converting a bicarbonate and/or carbonate ions to carbon dioxide and free base. Accordingly, in some embodiments, CA having an improved property (e.g., thermal stability, solvent stability, improved stability or activity in high concentrations of DA and/or base stability) that favors the reverse reaction is provided. Thus, in some embodiments the method of carbonic anhydrase catalyzed reverse reaction may be carried out at a temperature greater than 70° C., greater than 80° C. or greater than 105° C. In some embodiments, the temperature may be greater than 200° C. In still other embodiments, the temperature may be about 315° C. In some embodiments, the temperature is less than 315° C., less than 250° C. or less than 215° C. In still other embodiments, the temperature may be greater than 20° C., greater than 30° C., or greater than 40° C. and where a vacuum is used, greater than 70° C., greater than 80° C. or greater than 105° C. In some embodiments, the temperature may be greater than 200° C. In still other embodiments, the temperature may be about 315° C. In some embodiments, the temperature is less than 315° C., less than 250° C. or less than 215° C. In still other embodiments, the temperature may be greater than 20° C., greater than 30° C., or greater than 40° C. and where a vacuum is used.
(99) Conversion may also be carried out in a vacuum such as lower than atmospheric pressure (e.g. 0.01 to 1 atm). In other embodiments, the pressure may include pressure of about 1 to about 10 bar (within the vessel, not the inlet air pressure) or about 1 to about 3 bar. When temperature and pressure are used in combination, the pressure may be from about 1 to about 3 bar. In some embodiments, the temperature is less than 315, less than 250 or less than 215. The released CO2 may be recycled back into the system (e.g. into the cultured medium).
(100) Other examples of converting diamine-Carbonates and/or Carbamates (e.g. HMD) to diamine free base (e.g. HMD free base) include sparging with gas (e.g. air, or inert gas such as nitrogen or helium) or steam stripping. The steam can be added from an external source, or generated in situ by boiling the broth. In some embodiments converting diamine (e.g. HMD) Carbonates and/or Carbamates to free base include by heat and sparging with gas. In one embodiment, stripping may be carried out at a pressure of about 1 to about 10 bar. In some embodiments, the converting step can result in about at least 20% to about at least 99% diamine free base. In other embodiments, the converting step can result in about at least about 20-30%, 30-40%, or 40-50%.
(101) When sufficient heat is added to generate steam from the medium in situ in a stripping column, both water and carbon dioxide can be removed in sufficient amounts to obtain a solution concentrated in diamine (e.g. HMD) free base that allows efficient subsequent diamine recovery. In some embodiments, CA is also added to enhance the release of carbon dioxide from a solution of DA Carbonates or DA Carbamates by converting a bicarbonate and/or carbonate ions to carbon dioxide. Increasing the efficiency of CO2 removal from the DA Carbonate salt solution (e.g. HMD-bicarbonate salt solution) can lower purification costs by reducing the size of the stripping column.
(102) In some embodiments, the diamine recovered as diamine free base can be greater than 40% or greater than 50%. In still other embodiments, the diamine recovered can be greater than 50% in free base form from the stripping step under ambient pressure, air sparge, and high temperature (e.g. less than 315° C., less than 215° C., or around 115° C.).
(103) The addition of strong base (e.g. sodium or calcium hydroxide) may be added to raise the pH after diamine (e.g. HMD) free base is generated and CO2 is removed, thereby improving the extraction. If calcium hydroxide is used, a carbonate precipitate will form, which can then be separated from the liquid phase.
(104) Solids Removal Prior to Conversion
(105) Before converting the diamine (e.g. HMD) Carbonates and/or Carbamates to the diamine free base, solids can be separated from the cultured medium. Such solids may include cells and other biomass by products and impurities from the cultured medium. The resultant liquid fraction may be enriched in the diamine (e.g. HMD) Carbonates and/or Carbamates.
(106) Separation may be achieved by centrifugation, filtration, rotary drum or combinations thereof. Exemplary centrifugation may be by a disc-stack centrifuge or decanter or solid bowl centrifuge. It should be understood that any combination of centrifugation types or configurations and number of centrifugations may be used to achieve the desired solids separation from the culture medium. If solids are not separable by centrifugation or additional separation is required, separation by filtration may be used. Filtration may be achieved by ultrafiltration.
(107) Water Reduction or Removal
(108) In some embodiments, water may be removed or reduced after the solids removal and prior to conversion. Any known suitable process for water removal or reduction may be used such as for example, evaporation, reverse osmosis, or electrodialysis.
(109) In other embodiments, water may be removed prior to the step of isolating the diamine free base. One benefit of removing water before the isolation step can be an increase in pH. Methods to reduce or remove water as disclosed above in connection with water reduction or removal after solids removal and prior to conversion can also be used. The amount of water removal (e.g. upper limit of water removal) can depend on the solubility limit of a medium component or byproduct or the diamine salt or carbamate. In one embodiment, the water removal is dependent on whether it prevents insolubility of a medium component or byproduct, including the diamine salt or carbamate.
(110) Evaporation may be carried out with multiple effects evaporator, thermal vapor recompression or mechanical vapor recompression. An evaporator is a heat exchanger in which a liquid is boiled to give a vapor that is also a low pressure steam generator. This steam can be used for further heating in another evaporator called another “effect.” Thus, for example, two evaporators can be connected so that the vapor line from one is connected to the steam chest of the other providing a two, or double-effect evaporator. This configuration can be propagated to a third evaporator to create a triple-effect evaporator, for example.
(111) In one embodiment, the amount of water removed is 10 wt percent. The removed water can be further recovered and recycled such as in the culturing process of step a) or as shown in
(112) Simultaneous Removal of Water and Carbon Dioxide
(113) Water removal or reduction allows for a smaller solvent extraction column and less solvent, and the removal of carbon dioxide enables alkalization of the diamine composition (e.g. filtration permeate) to increase solvent extraction efficiency. In one embodiment described above, an evaporator is employed to remove sufficient water (and also enables removal of CO2) followed by a stripping column to remove any residual carbon dioxide (and can also remove water). Simultaneous removal of water and carbon dioxide in a single step to a point sufficient for subsequent extraction has the benefit of reducing costs and downtime associated with multiple steps. Accordingly, in another embodiment described above, a single step or unit operation is used to remove sufficient water and carbon dioxide (e.g. DIC) to enhance downstream diamine recovery, such as by solvent extraction. Accordingly, a step or unit operation (e.g. water evaporator or stripping column) and its associated costs of equipment, maintenance, use and risk of downtime may be absent or reduced. In one embodiment, the simultaneous water and carbon dioxide removal enhances the downstream recovery of HMD.
(114) Water removal will also enable carbon dioxide stripping. Conditions for water removal can allow sufficient carbon dioxide removal obviating a need for a separate CO2 removal step. Thus in one embodiment the simultaneous removal of water and CO2 is effectively achieved by either a stripping unit or an evaporator unit. For example, an evaporator, e.g. a multi-effect evaporator, a mechanical vapor recompressor, can be used to remove sufficient water and carbon dioxide to obtain a solution concentrated in diamine free base that allows efficient subsequent diamine recovery. For example, in
(115) It should be understood that the carbonic anhydrase may be present in a step or steps for releasing carbon dioxide and generating free DA base to enhance the release of carbon dioxide from a solution of DA Carbonates or DA Carbamates. In some embodiments, the CA may be present in the water removal or evaporation step, in other embodiments, the CA may be present in the CO2 stripping step, and in still other embodiments, the CA may be present in both the water removal or evaporation step and the CO2 stripping step.
(116) Isolation of Diamine Free Base
(117) Once converted, the DA (e.g. HMD) free base may be isolated from the cultured medium by extraction with an organic solvent. The isolated DA is separated from the organic solvent by a process such as distillation. Exemplary extraction solvents include alcohols, amines, ethers, alkanes and ketones. Exemplary extraction alcohols include C4 to C8 monohydric alcohols. In some embodiments, the extraction alcohols include hexanol, particularly 1-hexanol, isopentanol, or cyclohexanol, toluene or ethyl ether or mixtures thereof. Alkanes are suitable solvents as demonstrated in the Examples, particularly when HMD is the diamine. Alkanes, specifically hexane, may be used because of their extremely low water solubility. Hexane extracted little if any water and provided reasonable recovery of the available free base. Alkanes are therefore suitable solvents for use in recovery of diamine free base (e.g. HMD). Suitable alkanes include C5-C12, linear or branched. In one embodiment, both the diamine to be extracted and the alkane selected as solvent may have the same number of carbon atoms. Heptane is another suitable alkane, especially for HMD, which is further supported by the in silico modeling study below. Isomers of hexane and heptane are suitable. Hexane isomers are 2-methylpentane, 3-methylpentane, 2,2-dimethylbutane, and 2,3-dimethylbutane. Heptane isomers are 2-methylhexane, 3-methylhexane, 2,2-dimethylpentane, 2,3-dimethylpentane, 2,4-dimethylpentane, 3,3-dimethylpentane, 3-ethylpentane and 2,2,3-trimethylbutane. In some embodiments, the DA (e.g. HMD) free based can be directly distilled from the cultured medium.
(118) Any suitable solvent may be used. In some embodiments, the solvent can have boiling points higher than HMD free base or desired diamine free base, lower than water or any boiling point in between HMD free base (or desired diamine free base) and water (an intermediate boiling point).
(119) The DA (e.g. HMD) free base can be isolated from the medium or a DA-enriched fraction (e.g. when solids and/or water is removed before isolating) using an extraction solvent to provide an aqueous phase and a DA-free-base-containing organic phase.
(120) The organic phase (extract) can include at least 10%, at least 20%, at least 30%, at least 40%, or at least 50% by weight DA (e.g. HMD) in free base, Carbonate, and/or Carbamate form, but predominantly in free base form. Depending on the number of extractions, in some embodiments, the DA (e.g. HMD) in the extract can be greater than 90% by weight.
(121) The amount of DA free base that is extracted is about greater than 90% by weight. In some embodiments, the DA free base is HMD free base that is greater than 90% by weight.
(122) The efficiency of solvent extraction of diamine, e.g. HMD, free base increases with decrease in carbon dioxide concentration such as DIC as shown in the Examples. A decrease in carbon dioxide results in higher pH and higher concentration of recoverable free base form. In some embodiments, the aqueous diamine solution prior to solvent extraction contains no detectable carbon dioxide, less than 0.01% carbon dioxide, less than 0.1%, 0.2%, 0.3%, 0.4%, 0.5% or 1% or less than 5% carbon dioxide. In some embodiments, the aqueous diamine solution prior to solvent extraction contains no detectable DIC, less than 0.01% DIC, less than 0.1%, 0.2%, 0.3%, 0.4%, 0.5% or 1% or less than 5% DIC. In other embodiments, the aqueous DA solution prior to solvent extraction contains no detectable DIC, less than 0.01% DIC, less than 0.1%, 0.2%, 0.3%, 0.4%, 0.5% or 1% or less than 5% DIC.
(123) In one embodiment, CO2 produced stoichiometrically with DA, e.g. HMD, by an enzymatic pathway is used to neutralize DA, e.g. HMD, to maintain pH suitable for fermentation, generally a pH about 9 or lower. If desired or needed, further pH control can be achieved by supplementing with CO2 produced by the microbe as a by-product (e.g. shunt pyruvate to formate to CO2) or with an external source of CO2. The external CO2 can be purchased or can be CO2 recycled from the fermentation/isolation process. Due to the presence of CO2 during fermentation HMD carbonate, HMD bis-bicarbonate, HMD bicarbonate and a small amount of HMD carbamate and HMD biscarbamate are formed. At the end of fermentation, cells are optionally removed, and the culture medium is treated under pressure to degrade the HMD carbonate or carbamate compounds (or DA carbonate or carbamate compounds) releasing, for example, gaseous CO2 and creating free base HMD (neutral form 2HN—(CH2)6-NH2) (or DA free base) that increases the pH of the cultured medium. The free base or neutral HMD (or DA) may be isolated via solvent extraction. During the process, the released CO2 may be recycled. In one embodiment, the disclosed process does not require the use of acids to control pH and subsequent addition of base to neutralize the acids to generate solvent extractable free base HMD (or DA).
(124) In another embodiment of the process, the culture or cultured medium is thermally treated by heating to reflux temperature, for example either batch wise or continually, for example to 90-110° C. at atmospheric pressure, or to a higher temperature at overpressure.
(125) In another embodiment of the process, DA (e.g. HMD) is extracted with an organic solvent having a miscibility gap with water and stable at alkaline pH, such as in particular a polar, more specifically dipolar protic, organic solvent. Suitable solvents are as disclosed above.
(126) In one embodiment, DA (e.g. HMD) extraction is and/or subsequent phase separation is carried out batchwise at elevated temperature.
(127) The cultured medium, before or after removing the microbial organisms may be thickened or concentrated by known methods, such as, for example, with the aid of a rotary evaporator, thin-film evaporator, falling film evaporator, by reverse osmosis or by nanofiltration. If necessary, salts which may have precipitated due to the concentration procedure may be removed, for example by filtration or centrifugation. This concentrated cultured medium can then be worked up in the manner as disclosed herein to obtain DA (e.g. HMD). For the work up in accordance with the disclosed process, such a concentration procedure is feasible, but not absolutely necessary.
(128) According to an embodiment, DA (e.g. HMD) is extracted from the cultured medium with the aid of an organic solvent. The organic solvent may have, for example, a miscibility gap with water and stable at alkaline pH, such as in particular a polar, dipolar protic, organic solvent. Suitable solvents are in particular cyclic or open-chain, optionally branched alkanols having from 3 to 8 carbon atoms, in particular n- and iso-propanol, n-, sec- and iso-butanol, or cyclohexanol, and also n-pentanol, n-hexanol-n-heptanol, n-octanol, 2-octanol and the mono- or polybranched isomeric forms thereof.
(129) In one embodiment, the extraction and/or subsequent phase separation are carried out batchwise at an elevated temperature which is limited by the boiling points of water and of the extractant or of possibly forming azeotropes. Using for example, the extractant n-butanol extraction and phase separation could be carried out, for example, at about 25-90° C. or, preferably, at 40-70° C. For extraction, the two phases are stirred until the partition equilibrium has been established, for example over a period of from 10 seconds to 2 hours, or 5 to 15 min. The phases are then left to settle until they have separated completely; this takes for example, from 10 seconds to 5 hours, for example 15 to 120 or 30 to 90 minutes, in particular also at a temperature in the range from about 25-90° C. or 40-70° C. in the case of n-butanol.
(130) In further embodiments, DA (e.g. HMD) is extracted from the cultured medium continuously in a multi-stage process (for example in mixer-settler combinations) or continuously in an extraction column.
(131) One of skill in the art may establish the configuration of the extraction columns which can be employed according to the disclosed process for the phases to be separated in each case as part of optimization routines. Suitable extraction columns are in principle those without power input or those with power input, for example pulsed columns or columns with rotating internals. The skilled worker may also, as part of routine work, select in a suitable manner types and materials of internals, such as sieve trays, and column trays, to optimize phase separation. The basic theories of liquid-liquid extraction of small molecules are well known (cf. e.g. H.-J. Rehm and G. Reed, Eds., (1993), Biotechology, Volume 3 Bioprocessing, Chapter 21, VCH, Weinheim). The configuration of industrially applicable extraction columns is described, for example, in Lo et al., Eds., (1983) Handbook of Solvent Extraction, John Wiley & Sons, New York. Explicit reference is made to the disclosure of the textbooks above.
(132) After phase separation, DA (e.g. HMD) is isolated and purified from the DA-containing extract phase in a manner known per se. Possible measures of recovering DA (e.g. HMD) are in particular, without being limited thereto, distillation, precipitation as salt with suitable organic or inorganic acids, or combinations of such suitable measures.
(133) Distillation
(134) Distillation may be carried out continuously or batchwise. A single distillation column or a plurality of distillation columns coupled to one another may be used. Configuring the distillation column apparatus and establishing the operational parameters are the responsibilities of the skilled worker. The distillation columns used in each case may be designed in a manner known per se (see e.g. Sattler, Thermische Trennverfahren [Thermal separation methods], 2nd Edition 1995, Weinheim, p. 135ff; Perry's Chemical Engineers Handbook, 7th Edition 1997, New York, Section 13). Thus, the distillation columns used may have separation-effective internals, such as separation trays, e.g. perforated trays, bubble-cap trays or valve trays, arranged packings, e.g. sheet-metal or fabric packings, or random beds of packings. The number of plates required in the column(s) used and the reflux ratio are essentially governed by the purity requirements and the relative boiling position of the liquids to be separated, with the skilled worker being able to ascertain the specific design and operating data by known methods.
(135) In some embodiments, the distillation step substantially removes water and solvent. The temperature of distillation can be below 170 degrees C., below 160 degrees C., below 150 degrees C., or below 140 Degrees C.
(136) Precipitation as salt may be achieved by adding suitable organic or inorganic acids, for example sulfuric acid, hydrochloric acid, phosphoric acid, acetic acid, formic acid, carbonic acid, oxalic acid, etc. In another preferred embodiment, an organic dicarboxylic acid is used, forming a salt which can be used, either directly or after purification, for example by recrystallization, in a subsequent polycondensation to give the polyamide. More specifically, such dicarboxylic acids are C4-C12-dicarboxylic acids.
(137) The organic DA (e.g. HMD) phase produced in the extraction procedure may also be worked up chromatographically. For chromatography, the DA phase is applied to a suitable resin, for example a strongly or weakly acidic ion exchanger (such as Lewatit 1468 S, Dowex Marathon C, Amberlyst 119 Wet or others), with the desired product or the contaminants being partially or fully retained on the chromatographic resin. These chromatographic steps may be repeated, if necessary, using the same or other chromatographic resins. The skilled worker is familiar with selecting the appropriate chromatographic resins and their most effective application. The purified product may be concentrated by filtration or ultrafiltration and stored at an appropriate temperature.
(138) The identity and purity of the compound(s) isolated may be determined by known technologies. These include high performance liquid chromatography (HPLC), gas chromatography (GC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzyme assay or microbiological assays. These analytical methods are summarized in: Patek et al. (1994) Appl. Environ. Microbiol. 60:133-140; Malakhova et al. (1996) Biotekhnologiya 11 27-32; and Schmidt et al. (1998) Bioprocess Engineer. 19:67-70. Ullmann's Encyclopedia of Industrial Chemistry (1996) Vol. A27, VCH: Weinheim, pp. 89-90, pp. 521-540, pp. 540-547, pp. 559-566, 575-581 and pp. 581-587; Michal, G (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al. (1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, Vol. 17.
(139) The disclosed process may include various combinations of steps or processes as depicted in
(140) 1. A fermentor or any vessel in which a microorganism may be cultured or grown in a suitable medium under suitable conditions and for a sufficient period of time to form one or more of diamine Carbonates and/or Carbamates in a cultured medium in the presence of carbon dioxide, carbonate, bicarbonate or carbonic acid.
(141) 2. Microbial Heat Kill/Conversion: Many processes require inactivation of the microbial culture post-fermentation. Once the diamine Carbonates and/or Carbamates are formed, they may be converted to the free base where CO2 is released if sterilization/heat kill step occurs at elevated temperatures.
(142) 3: Solids removal: The solids from the cultured medium may be optionally removed before the diamine Carbonates and/or Carbamates are converted to release CO2.
(143) 4: Conversion (all possible ways to release CO2). The released CO2 may be recycled to fermentor.
(144) 5: Water Removal: removing water from the DA free base mixture, and optional recycling water and/or carbon dioxide to the fermentor;
(145) 6: Solvent Extraction, extracting the DA mixture with organic solvent in an extractor to form an organic phase DA solution and aqueous raffinate, and optionally recycling the aqueous raffinate to the conversion step;
(146) 7: Purification: Involves distillation which optionally recycles organic solvent back to the solvent extraction step, and purification could involve more distillation columns to purify the diamine and other steps to remove color forming compounds and the like.
(147) 8: Purified DA: the resulting purified DA free base from the steps above;
(148) 9: Optional Microbial Heat Kill, where no CO2 is released
(149) 10: Optional water removal, recycle of water and possible CO2 if released removing water from the Carbonates and/or Carbamates mixture, and optional recycling water and/or carbon dioxide to the fermentor;
(150) 11: Optional direct purification from aqueous phase with or without release of CO2, could involve distillation, ion exchange, electrodialysis, and other suitable processes or steps. Possible to recycle water and CO2 if produced in these steps: optionally directly converting the Carbonates and/or Carbamates from the cultured medium to form a DA free base mixture, and optionally releasing of water and/or carbon dioxide that may be recycled to the fermentor;
(151) 12: Alkalization (NaOH or CaOH) or other steps to remove Carbonates from HMD (Ion exchange, electrodialysis, etc.): adding an aqueous base to the remove the Carbonates and/or Carbamates from the DA free base mixture.
(152) Referring to
(153) The disclosed process can be applied in principle using any diamine-containing cultured medium (e.g. HMD-containing cultured medium). There are also in principle no limitations whatsoever regarding the microorganisms employed in the culturing or fermentation. The microorganism may be naturally occurring microorganisms; microorganisms improved by means of mutation and selection, and recombinantly produced or genetically engineered microorganisms, such as bacteria and fungi. These microorganisms are capable either of producing a DA or DA derivative, HMD and/or HMD derivatives such as HMD carbonate or HMD bicarbonate. More specifically, a recombinant organism employed is capable of DA biosynthesis, e.g. HMD biosynthesis via the HMD pathways (“HMD pathway”) discussed below and as disclosed in U.S. Pat. No. 8,377,680, or other references cited herein, which disclosures are hereby incorporated by reference in its entirety.
(154) In some embodiments, the genetically engineered microorganism that has a DA pathway including at least one exogenous nucleic acid encoding at least one enzyme of the DA pathway can also include an exogenous nucleic acid encoding a carbonic anhydrase enzyme. In other embodiments, the genetically engineered microorganism has a DA synthesis pathway, preferably an HMD synthesis pathway, with at least two exogenous nucleic acids encoding at least one enzyme of the DA synthesis pathway, preferably HMD synthesis pathway, and a carbonic anhydrase enzyme or variant expressed in a sufficient amount to produce at least one DA Carbonates and/or DA Carbamates, preferably HMD Carbonates and/or Carbamates, compound. In other embodiments, the genetically engineered microorganism has a DA synthesis pathway, preferably an HMD synthesis pathway, with at least two exogenous nucleic acids encoding at least one enzyme of the DA synthesis pathway, preferably the HMD synthesis pathway, and a carbonic anhydrase enzyme or variant expressed in a sufficient amount to produce at least one or more DA free base, preferably HMD free base, and carbon dioxide. It should be understood that a process for a diamine production can include the genetically engineered microorganisms as discussed above and that has a DA pathway, preferably HMD synthesis pathway, and a carbonic anhydrase enzyme or variant expressed in a sufficient amount to produce at least one DA Carbonates and/or DA Carbamates, preferably HMD Carbonates and/or Carbamates, compound or that can produce, at least one or more DA free base, preferably HMD free base, and carbon dioxide or both.
(155) Exemplary HMD synthesis pathways include pathways depicted in
(156) Any of the disclosed HMD synthesis pathways may be used to generate a genetically engineered microorganism that produces the pathway, pathway intermediate or product as desired. For example, the genetically engineered microorganism can have a HMD pathway including at least one exogenous nucleic acid encoding at least one enzyme of the HMD synthesis pathway expressed in a sufficient amount to produce at least one HMD Carbonates and/or Carbamates compound. The genetically engineered microorganism can have a HMD pathway including at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten, or at least eleven exogenous nucleic acids encoding enzymes of the HMD synthesis pathway. The exogenous nucleic acids can encode, for example, polypeptides, where the polypeptide is an enzyme or protein that can convert desired substrates, intermediates and produce products of the desired HMD synthesis pathways.
(157) In some embodiments, the genetically engineered microorganism that has a HMD pathway including at least one exogenous nucleic acid encoding at least one enzyme of the HMD can also include an exogenous nucleic acid encoding a carbonic anhydrase enzyme.
(158) For example, the HMD synthesis pathway may include intermediates such as 3-oxoadipyl-CoA, adipate semialdehyde, 6-aminocaproate (6-ACA), 6-ACA semialdehyde, 2-aminopimelate, 3,6-dihydroxyhexanoyl-CoA and homolysine.
(159) In some embodiments, the HMD synthesis pathway may include enzymes such as 3-oxoadipyl-CoA thiolase, 6-ACA transaminase or dehydrogenase, 6-aminocaproyl-CoA reductase, 6-ACA reductase, adipyl-CoA reductase, adipate reductase, 6-hydroxy 3-oxohexanoyl-CoA dehydrogenase, 2-aminopimelate decarboxylase, and homolysine decarboxylase.
(160) In other embodiments, the HMD synthesis pathway may include an enzyme and substrate-product pair such as 3-oxoadipyl-CoA thiolase that acts on succinyl-CoA and acetyl-CoA to make 3-oxoadipyl-CoA, 6-ACA transaminase that acts on adipyl-CoA to form 6-ACA, 6-aminocaproyl-CoA reductase that acts on 6-aminocaproyl-CoA to form 6-ACA semialdehyde, 6-ACA reductase that acts on 6-ACA and converts it directly to 6-ACA semialdehyde, adipyl-CoA reductase that acts on adipyl-CoA to form adipate semialdehyde, adipate reductase that acts on adipate and converts it directly to adipate semialdehyde, 6-hydroxy 3-oxohexanoyl-CoA dehydrogenase that reduces 6-hydroxy 3-oxohexanoyl-CoA to form 3,6-dihydroxy hexanoyl-CoA, 2-aminopimelate decarboxylase that decarboxylates 2-aminopimelate to form 6-ACA, and homolysine decarboxylase that decarboxylates homolysine to form HMDA.
(161) In some embodiments, a microorganism may produce the desired diamine (e.g. HMD) via a desired synthesis pathway that may include the following:
(162) (a) 3-oxoadipyl-CoA thiolase, 3-oxoadipyl-CoA dehydrogenase, 3-hydroxyadipyl-CoA dehydratase, 5-carboxy-2-pentenoyl-CoA reductase, adipyl-CoA reductase, 6-ACA transaminase or dehydrogenase, 6-ACA transferase or synthetase and 6-ACA-CoA reductase, or 6-ACA reductase, HMDA transaminase or dehydrogenase;
(163) (b) 3-oxoadipyl-CoA thiolase, 3-oxoadipyl-CoA dehydrogenase, 3-hydroxyadipyl-CoA dehydratase, 5-carboxy-2-pentenoyl-CoA reductase, adipyl-CoA reductase, 6-ACA transaminase or dehydrogenase, 6-ACA reductase, HMDA transaminase or dehydrogenase;
(164) (c) 3-oxoadipyl-CoA thiolase, 3-oxoadipyl-CoA dehydrogenase, 3-hydroxyadipyl-CoA dehydratase, 5-carboxy-2-pentenoyl-CoA reductase, adipyl-CoA transferase, hydrolase or transferase, adipate reductase, 6-ACA transaminase or dehydrogenase, 6-ACA transferase or synthetase, 6-ACA-CoA reductase, HMDA transaminase or dehydrogenase;
(165) (d) 3-oxoadipyl-CoA thiolase, 3-oxoadipyl-CoA dehydrogenase, 3-hydroxyadipyl-CoA dehydratase, 5-carboxy-2-pentenoyl-CoA reductase, adipyl-CoA transferase, hydrolase or transferase, adipate reductase, 6-ACA transaminase or dehydrogenase, 6-ACA reductase, HMDA transaminase or dehydrogenase;
(166) (e) 3-oxoadipyl-CoA thiolase, 3-oxoadipate dehydrogenase, 3-hydroxyadipate dehydratase, 5-carboxy-2-pentenoate reductase, adipate reductase, 6-ACA transaminase or dehydrogenase, 6-ACA transferase or synthetase, 6-ACA-CoA reductase, HMDA transaminase or dehydrogenase;
(167) (f) 3-oxoadipyl-CoA thiolase, 3-oxoadipate dehydrogenase, 3-hydroxyadipate dehydratase, 5-carboxy-2-pentenoate reductase, adipate reductase, 6-ACA transaminase or dehydrogenase, 6-ACA reductase, HMDA transaminase or dehydrogenase;
(168) (g) 3-oxoadipyl-CoA thiolase, 3-oxoadipate dehydrogenase, 3-hydroxyadipate dehydratase, 5-carboxy-2-pentenoate reductase, adipyl-CoA transferase, hydrolase or transferase, adipyl-CoA reductase, 6-ACA transaminase or dehydrogenase, 6-ACA transferase or synthetase, 6-ACA-CoA reductase, HMDA transaminase or dehydrogenase;
(169) (h) 3-oxoadipyl-CoA thiolase, 3-oxoadipate dehydrogenase, 3-hydroxyadipate dehydratase, 5-carboxy-2-pentenoate reductase, adipyl-CoA transferase, hydrolase or transferase, adipyl-CoA reductase, 6-ACA transaminase or dehydrogenase, 6-ACA reductase, HMDA transaminase or dehydrogenase;
(170) (i) an 4-hydroxy-2-oxoheptane-I,7-dioate (HODH aldolase); an 2-oxohept-4-ene-I,7-dioate (OHED) hydratase; an OHED formate-lyase and a pyruvate formate-lyase activating enzyme or OHED dehydrogenase; a 2,3-dehydroadipyl-CoA reductase; an adipyl-CoA dehydrogenase; or an adipate semialdehyde aminotransferase or an adipate semialdehyde oxidoreductase (aminating);
(171) (j) a β-ketothiolase or an acetyl-CoA carboxylase and an acetoacetyl-CoA synthase, a 3-hydroxyacyl-CoA dehydrogenase or a 3-oxoacyl-CoA reductase, an enoyl-CoA hydratase, and a trans-2-enoyl-CoA reductase for producing hexanoyl-CoA, one or more of a thioesterase, an aldehyde dehydrogenase, or a butanal dehydrogenase, said host producing hexanal or hexanoates; one or more of a monooxygenase, an alcohol dehydrogenase, an aldehyde dehydrogenase, a 6-hydroxyhexanoate dehydrogenase, a 5-hydroxypentanoate dehydrogenase, a 4-hydroxybutyrate dehydrogenase, a 6-oxohexanoate dehydrogenase, or a 7-oxoheptanoate dehydrogenase, said host producing adipic acid or adipate semialdehyde; one or more of a monooxygenase, a transaminase, a 6-hydroxyhexanoate dehydrogenase, a 5-hydroxypentanoate dehydrogenase, a 4-hydroxybutyrate dehydrogenase, and an alcohol dehydrogenase, said host producing 6-aminohexanoate; one or more of a carboxylate reductase, a w-transaminase, a deacetylase, a N-acetyl transferase, or an alcohol dehydrogenase. Such pathway is disclosed in in U.S. Patent Application Publication No. 20140186902;
(172) (k) acetyltransferase or thiolase to form 6-hydroxy-3-oxo-hexanoyl-CoA, 6-hydroxy-3-oxo-hexanoyl-CoA dehydrogenase, 3,4-dihydroxyhexanoyl-CoA dehydratase, 6-hydroxy-2-hexenoyl-CoA reductase, 6-hydroxyhexanoyl-CoA hydrolase to form 6-ACA, 6-hydroxycaproate dehydrogenase and transaminase to form HMDA. Such pathway is disclosed in International Application Publication No. WO 2014/047407A1;
(173) (l) homocitrate synthase, a homoaconitase and a homoisocitrate dehydrogenase to form 2-ketopimelate, 2-keto decarboxylase catalyzing the conversion of α-ketopimelate to adipate semialdehyde, 2-aminotransferase catalyzes the conversion of α-ketopimelate to 2-aminopimelate, 2-aminopimelate decarboxylase to decarboxylate 2-aminopimelate and form 6-ACA, aldehyde dehydrogenase catalyzes the conversion of 6-ACA to 6-aminohexanal and the aminotransferase catalyzes the conversion of 6-aminohexanal to 6-hexamethylenediamine. Such pathway is disclosed in International Application Publication No. in WO/2010/068944; and
(174) (m) glutamyl-CoA transferase and/or ligase, beta-ketothiolase, 3-oxo-6-aminopimeloyl-CoA oxidoreductase, 3-hydroxy-6-aminopimeloyl-CoA dehydratase, 6-amino-7-carboxyhept-2-enoyl-CoA reductase, 6-aminopimeloyl-CoA reductase (aldehyde forming), 2-amino-7-oxoheptanoate aminotransferase and/or aminating oxidoreductase, homolysine decarboxylase, 6-aminopimeloyl-CoA hydrolase, transferase and/or ligase, 2-aminopimelate decarboxylase. Such pathway is disclosed in International Application Publication No. WO 2010/129936 In some embodiments, the HMD synthesis pathway includes at least one enzyme and the nucleic acids encoding such one or more enzymes for 3-oxoadipyl-CoA thiolase, 3-oxoadipyl-CoA dehydrogenase, 3-hydroxyadipyl-CoA dehydratase, 5-carboxy-2-pentenoyl-CoA reductase, adipyl-CoA reductase, 6-ACA transaminase or dehydrogenase, 3-oxoadipyl-CoA:acyl CoA transferase, 3-oxoadipate dehydrogenase, 3-hydroxyadipate dehydratase, 5-carboxy-2-pentenoate reductase, adipyl-CoA transferase, ligase, or hydrolase, 6-ACA transferase or synthetase, 6-ACA-CoA reductase, HMDA transaminase or dehydrogenase, adipate reductase, 6-ACA transaminase or dehydrogenase, or 6-ACA reductase.
(175) Suitable microorganisms that can be used as a host to include one or more exogenous nucleic acids of the HMD synthesis pathways include for example prokaryotes such as bacteria, and eukaryotes such as, fungus (e.g. yeast) or any other microorganisms applicable to fermentation processes or that can tolerate pH conditions below pH 4.
(176) In some embodiments, the genetically engineered microorganism Escherichia, Klebsiella; the order Aeromonadales, family Succinivibrionaceae, including the genus Anaerobiospirillum; the order Pasteurellales, family Pasteurellaceae, including the genera Actinobacillus and Mannheimia; the order Rhizobiales, family Bradyrhizobiaceae, including the genus Rhizobium; the order Bacillales, family Bacillaceae, including the genus Bacillus; the order Actinomycetales, families Corynebacteriaceae and Streptomycetaceae, including the genus Corynebacterium and the genus Streptomyces, respectively; order Rhodospirillales, family Acetobacteraceae, including the genus Gluconobacter; the order Sphingomonadales, family Sphingomonadaceae, including the genus Zymomonas; the order Lactobacillales, families Lactobacillaceae and Streptococcaceae, including the genus Lactobacillus and the genus Lactococcus, respectively; the order Clostridiales, family Clostridiaceae, genus Clostridium; and the order Pseudomonadales, family Pseudomonadaceae, including the genus Pseudomonas, the genus Alkaliphilus, Methylobacterium, Methyloversatilis, Methylococcus, Methylocystis and Hyphomicrobium the order Saccharomycetales, family Saccaromycetaceae, including the genera Saccharomyces, Kluyveromyces and Pichia; the order Saccharomycetales, family Dipodascaceae, including the genus Yarrowia; the order Schizosaccharomycetales, family Schizosaccaromycetaceae, including the genus Schizosaccharomyces; the order Eurotiales, family Trichocomaceae, including the genus Aspergillus; and the order Mucorales, family Mucoraceae, including the genus Rhizopus.
(177) In other embodiments the genetically engineered microorganism comprises non-limiting species of host bacteria include Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Corynebacterium glutamicum, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, and Pseudomonas putida, Bacillis pseudofirmus, Bacillus halodurans, Bacillus alcalophilus, Clostridium paradoxum, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Hansenula polymorpha, Pichia methanolica, Candida boidinii, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, Rhizopus oryzae, Yarrowia lipolytica, and Issatchenkia orientalis. Some alkaliphiles are: Bacillus pseudofirmus, Bacillus halodurans, Bacillus alcalophilus, Clostridium paradoxum, Arthrospira platensis, Bacillus clausii, Oceanobacillus iheyensis, Alkaliphilus metalliredigens, Alkaliphilus oremlandii, Bacillus selentireducens, Desulfovibrio alkaliphiles, Dethiobacter alkaliphiles, Thioalkalivibrio sp., Natranaerobius thermophilus, Alkalilimnicola ehrlichii, and Desulfonatronospira thiodismutans.
(178) In some embodiments, the species of fungi or yeast can be selected from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Hansenula polymorpha, Pichia methanolica, Candida boidinii, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, Rhizopus oryzae, Yarrowia lipolytica, and Issatchenkia orientalis and the like.
(179) In some embodiments, the genetically engineered microorganism is Escherichia coli, Corynebacterium glutamicum, Bacillus subtilis, Pseudomonas putida, Bacillis pseudofirmus, Bacillus halodurans, Bacillus alcalophilus, Clostridium paradoxum, Saccharomyces cerevisiae.
(180) For example, E. coli is a particularly useful host organism since it is a well characterized microbial organism suitable for genetic engineering. Other particularly useful host organisms include yeast such as Saccharomyces cerevisiae. In some embodiments, genetically engineered microorganisms are modified that have improved alkali tolerance.
(181) Alkali tolerance can be introduced into organisms that are not typically alkali-tolerant using adaptive evolution. Cells are grown under increasingly higher pH conditions till they have adapted their cellular mechanisms to grow optimally at the desired high pH (alkaline pH). Typically, small volumes of cells, still growing in exponential phase, are transferred to a fresh medium at a predetermined pH till they have reached a certain biomass concentration. These cells are then diluted into a fresh medium where an incrementally higher pH is maintained. This process selects for cells that are more fit to grow at the higher pH. The process of transferring small volume of exponentially growing cells into fresh medium at higher pH is continued till the cells have evolved to grow at the target pH level.
(182) Adaptive evolution has been used to evolve strains to grow on non-natural substrates (Lee and Palsson, Appl Environ Microbiol. 2010 July; 76(13):4158-68, Adaptive evolution of Escherichia coli K-12 MG1655 during growth on a Nonnative carbon source, L-1,2-propanediol), for improved salt tolerance (Ketola and Hiltunen, Ecol Evol. 2014 October; 4:3901-8, Rapid evolutionary adaptation to elevated salt concentrations in pathogenic freshwater bacteria Serratia marcescens), for improved product tolerance (Kildegaard K R et al., Metab Eng. 2014 Sep. 28; 26C: 57-66, Evolution reveals a glutathione-dependent mechanism of 3-hydroxypropionic acid tolerance), for growth at high temperature (Sandeberg et al., Mol Biol Evol. 2014 October; 31(10):2647-62, Evolution of Escherichia coli to 42° C. and subsequent genetic engineering reveals adaptive mechanisms and novel mutations), to evolve for fermentation under aerobic conditions (Portnoy et al., Appl Environ Microbiol. 2008 December; 74(24), Aerobic fermentation of D-glucose by an evolved cytochrome oxidase-deficient Escherichia coli strain) among several other objectives.
(183) For example, one of the pathways entails the activation of 6-aminocaproate to 6-aminocaproyl-CoA by a transferase or synthase enzyme (
(184) Another option for minimizing or even eliminating the formation of the cyclic imine or caprolactam during the conversion of 6-aminocaproic acid to HMD entails adding a functional group (for example, acetyl, succinyl) to the amine group of 6-aminocaproic acid to protect it from cyclization. This is analogous to ornithine formation from L-glutamate in Escherichia coli. Specifically, glutamate is first converted to N-acetyl-L-glutamate by N-acetylglutamate synthase. N-Acetyl-L-glutamate is then activated to N-acetylglutamyl-phosphate, which is reduced and transaminated to form N-acetyl-L-ornithine. The acetyl group is then removed from N-acetyl-L-ornithine by N-acetyl-L-ornithine deacetylase forming L-ornithine. Such a route is necessary because formation of glutamate-5-phosphate from glutamate followed by reduction to glutamate-5-semialdehyde leads to the formation of (S)-1-pyrroline-5-carboxylate, a cyclic imine formed spontaneously from glutamate-5-semialdehyde. In the case of forming HMD from 6-aminocaproic acid, the steps can involve acetylating 6-aminocaproic acid to acetyl-6-aminocaproic acid, activating the carboxylic acid group with a CoA or phosphate group, reducing, aminating, and deacetylating.
(185) Note that 6-aminocaproate can be formed from various starting molecules. For example, the carbon backbone of 6-aminocaproate can be derived from succinyl-CoA and acetyl-CoA as depicted in
(186)
(187) The starting molecule, 4-aminobutyryl-CoA, can be formed from various common central metabolites. For example, glutamate can be decarboxylated to 4-aminobutyrate, which is then activated by a CoA-transferase or synthase to 4-aminobutyryl-CoA. Alternatively, succinate semialdehyde, formed from either the reduction of succinyl-CoA or the decarboxylation of alpha-ketoglutarate, can be transaminated to 4-aminobutyrate prior to activation by a CoA-transferase or synthase to form 4-aminobutyryl-CoA. It is noted that 4-aminobutyryl-CoA and several of the intermediates of the 4-aminobutyryl-CoA to 6-aminocaproyl-CoA pathway may spontaneously cyclize to their corresponding lactams. Thus, adding a protective functional group to the terminal amine group of 4-aminobutyryl-CoA and/or several of the amino-CoA intermediates can be used to minimize the formation of unwanted cyclic byproducts. In this case, the same general set of transformations depicted in
(188) All transformations depicted in
(189) TABLE-US-00003 TABLE 8 Enzyme types for conversion of succinyl-CoA, acetyl-CoA, and/or 4-aminobutyryl-CoA to 6-amino- caproate, caprolactam, and/or hexamethylenediamine. The first three digits of each label correspond to the first three Enzyme Commission number digits which denote the general type of transformation independent of substrate specificity. Label Function 1.1.1.a Oxidoreductase (ketone to hydroxyl or aldehyde to alcohol) 1.2.1.b Oxidoreductase (acyl-CoA to aldehyde) 1.3.1.a Oxidoreductase operating on CH—CH donors 1.4.1.a Oxidoreductase operating on amino acids 2.3.1.b Acyltransferase 2.6.1.a Aminotransferase 2.8.3.a Coenzyme-A transferase 3.1.2.a Thiolester hydrolase (CoA specific) 4.2.1.a Hydro-lyase 6.2.1.a Acid-thiol ligase 6.3.1.a/ Amide synthases/peptide synthases 6.3.2.a No enzyme Spontaneous cyclization required
(190) 1.1.1.a Oxidoreductases. Four transformations depicted in
(191) Exemplary enzymes that can convert 3-oxoacyl-CoA molecules such as 3-oxoadipyl-CoA and 3-oxo-6-aminohexanoyl-CoA into 3-hydroxyacyl-CoA molecules such as 3-hydroxyadipyl-CoA and 3-hydroxy-6-aminohexanoyl-CoA, respectively, include enzymes whose natural physiological roles are in fatty acid beta-oxidation or phenylacetate catabolism. For example, subunits of two fatty acid oxidation complexes in E. coli, encoded by fadB and fadJ, function as 3-hydroxyacyl-CoA dehydrogenases (Binstock et al., Methods Enzymol. 71:403-411 (1981)). Furthermore, the gene products encoded by phaC in Pseudomonas putida U (Olivera et al., Proc. Natl. Acad. Sci. USA 95:6419-6424 (1998)) and paaC in Pseudomonas fluorescens ST (Di Gennaro et al., Arch. Microbiol. 188:117-125 (2007)) catalyze the reverse reaction of step B in
(192) TABLE-US-00004 Gene GenBank name Gl # Accession # Organism fadB 119811 P21177.2 Escherichia coli fadJ 3334437 P77399.1 Escherichia coli paaH 16129356 NP_415913.1 Escherichia coli phaC 26990000 NP_745425.1 Pseudomonas putida paaC 106636095 ABF82235.1 Pseudomonas fluorescens
(193) Additional exemplary oxidoreductases capable of converting 3-oxoacyl-CoA molecules to their corresponding 3-hydroxyacyl-CoA molecules include 3-hydroxybutyryl-CoA dehydrogenases. The enzyme from Clostridium acetobutylicum, encoded by hbd, has been cloned and functionally expressed in E. coli (Youngleson et al., J. Bacteriol. 171:6800-6807 (1989)). Additional gene candidates include Hbd1 (C-terminal domain) and Hbd2 (N-terminal domain) in Clostridium kluyveri (Hillmer et al., FEBS Lett. 21:351-354 (1972)) and HSD17B10 in Bos taurus (Wakil et al., J. Biol. Chem. 207:631-638 (1954)). Yet other gene candidates demonstrated to reduce acetoacetyl-CoA to 3-hydroxybutyryl-CoA are phbB from Zoogloea ramigera (Ploux et al., Eur. J. Biochem. 174:177-182 (1988)) and phaB from Rhodobacter sphaeroides (Alber et al., Mol. Microbiol 61:297-309 (2006)). The former gene candidate is NADPH-dependent, its nucleotide sequence has been determined (Peoples et al., Mol. Microbiol. 3:349-357 (1989)) and the gene has been expressed in E. coli. Substrate specificity studies on the gene led to the conclusion that it could accept 3-oxopropionyl-CoA as a substrate besides acetoacetyl-CoA (Ploux et al., supra).
(194) TABLE-US-00005 Gene Gen Bank name Gl # Accession # Organism hbd 18266893 P52041.2 Clostridium acetobutylicum Hbd2 146348271 EDK34807.1 Clostridium kluyveri Hbd1 146345976 EDK32512.1 Clostridium kluyveri HSD17B10 3183024 O02691.3 Bos taurus phbB 130017 P23238.1 Zoogloea ramigera phaB 146278501 YP_001168660.1 Rhodobacter sphaeroides
(195) A number of similar enzymes have been found in other species of Clostridia and in Metallosphaera sedula (Berg et al., Science 318:1782-1786 (2007)).
(196) TABLE-US-00006 GenBank Gene name Gl # Accession # Organism hbd 15895965 NP_349314.1 Clostridium acetobutylicum hbd 20162442 AAM14586.1 Clostridium beijerinckii Msed_1423 146304189 YP_001191505 Metallosphaera sedula Msed_0399 146303184 YP_001190500 Metallosphaera sedula Msed_0389 146303174 YP_001190490 Metallosphaera sedula Msed_1993 146304741 YP_001192057 Metallosphaera sedula
(197) Various alcohol dehydrogenases represent good candidates for converting 3-oxoadipate to 3-hydroxyadipate (step H,
(198) TABLE-US-00007 Gene GenBank name Gl # Accession # Organism mdh 1789632 AAC76268.1 Escherichia coli IdhA 16129341 NP_415898.1 Escherichia coli ldh 113866693 YP_725182.1 Ralstonia eutropha bdh 177198 AAA58352.1 Homo sapiens adh 60592974 AAA23199.2 Clostridium beijerinckii adh 113443 P14941.1 Thermoanaerobacter brockii
(199) 1.2.1.b Oxidoreductase (acyl-CoA to aldehyde). The transformations of adipyl-CoA to adipate semialdehyde (Step N,
(200) TABLE-US-00008 Gene GenBank name Gl # Accession # Organism acr1 50086359 YP_047869.1 Acinetobacter calcoaceticus acr1 1684886 AAC45217 Acinetobacter baylyi acr1 18857901 BAB85476.1 Acinetobacter sp. Strain M-1 sucD 172046062 P38947.1 Clostridium kluyveri sucD 34540484 NP_904963.1 Porphyromonas gingivalis bphG 425213 BAA03892.1 Pseudomonas sp adhE 55818563 AAV66076.1 Leuconostoc mesenteroides
(201) An additional enzyme type that converts an acyl-CoA to its corresponding aldehyde is malonyl-CoA reductase which transforms malonyl-CoA to malonic semialdehyde. Malonyl-CoA reductase is a key enzyme in autotrophic carbon fixation via the 3-hydroxypropionate cycle in thermoacidophilic archaeal bacteria (Berg et al., supra; Thauer R. K., Science 318:1732-1733 (2007)). The enzyme utilizes NADPH as a cofactor and has been characterized in Metallosphaera and Sulfolobus spp (Alber et al., J. Bacteriol. 188:8551-8559 (2006); Hugler et al., J. Bacteriol. 184:2404-2410 (2002)). The enzyme is encoded by Msed_0709 in Metallosphaera sedula (Alber et al., supra; Berg et al., supra). A gene encoding a malonyl-CoA reductase from Sulfolobus tokodaii was cloned and heterologously expressed in E. coli (Alber et al., supra). This enzyme has also been shown to catalyze the conversion of methylmalonyl-CoA to its corresponding aldehyde (WO/2007/141208). Although the aldehyde dehydrogenase functionality of these enzymes is similar to the bifunctional dehydrogenase from Chloroflexus aurantiacus, there is little sequence similarity. Both malonyl-CoA reductase enzyme candidates have high sequence similarity to aspartate-semialdehyde dehydrogenase, an enzyme catalyzing the reduction and concurrent dephosphorylation of aspartyl-4-phosphate to aspartate semialdehyde. Additional gene candidates can be found by sequence homology to proteins in other organisms including Sulfolobus solfataricus and Sulfolobus acidocaldarius and have been listed below. Yet another candidate for CoA-acylating aldehyde dehydrogenase is the ald gene from Clostridium beijerinckii (Toth et al., Appl Environ Microbiol 65:4973-4980 (1999)). This enzyme has been reported to reduce acetyl-CoA and butyryl-CoA to their corresponding aldehydes. This gene is very similar to eutE that encodes acetaldehyde dehydrogenase of Salmonella typhimurium and E. coli (Toth et al., supra).
(202) TABLE-US-00009 GenBank Gene name Gl # Accession # Organism Msed_0709 146303492 YP_001190808.1 Metallosphaera sedula mcr 15922498 NP_378167.1 Sulfolobus tokodaii asd-2 15898958 NP_343563.1 Sulfolobus solfataricus Saci_2370 70608071 YP_256941.1 Sulfolobus acidocaldarius Ald 49473535 AAT66436 Clostridium beijerinckii eutE 687645 AAA80209 Salmonella typhimurium eutE 2498347 P77445 Escherichia coli
(203) 1.3.1.a Oxidoreductase operating on CH—CH donors. Referring to
(204) TABLE-US-00010 Gene Gen Bank name Gl # Accession # Organism bcd 15895968 NP_349317.1 Clostridium acetobutylicum etfA 15895966 NP_349315.1 Clostridium acetobutylicum etfB 15895967 NP_349316.1 Clostridium acetobutylicum TER 62287512 Q5EU90.1 Euglena gracilis TDE0597 42526113 NP_971211.1 Treponema denticola
(205) Step J of both
(206) TABLE-US-00011 Gene Gen Bank name Gl # Accession # Organism fadH 16130976 NP_417552.1 Escherichia coli enr 169405742 ACA54153.1 Clostridium botulinum A3 str enr 2765041 CAA71086.1 Clostridium tyrobutyricum enr 3402834 CAA76083.1 Clostridium kluyveri enr 83590886 YP_430895.1 Moorella the rmoacetica
(207) 1.4.1.a Oxidoreductase operating on amino acids.
(208) Most oxidoreductases operating on amino acids catalyze the oxidative deamination of alpha-amino acids with NAD+ or NADP+ as acceptor, though the reactions are typically reversible. Exemplary oxidoreductases operating on amino acids include glutamate dehydrogenase (deaminating), encoded by gdhA, leucine dehydrogenase (deaminating), encoded by ldh, and aspartate dehydrogenase (deaminating), encoded by nadX. The gdhA gene product from Escherichia coli (McPherson et al., Nucleic. Acids Res. 11:5257-5266 (1983); Korber et al., J. Mol. Biol. 234:1270-1273 (1993)), gdh from Thermotoga maritima (Kort et al., Extremophiles 1:52-60 (1997); Lebbink et al., J. Mol. Biol. 280:287-296 (1998); Lebbink et al., J. Mol. Biol. 289:357-369 (1999)), and gdhA1 from Halobacterium salinarum (Ingoldsby et al., Gene. 349:237-244 (2005)) catalyze the reversible interconversion of glutamate to 2-oxoglutarate and ammonia, while favoring NADP(H), NAD(H), or both, respectively. The ldh gene of Bacillus cereus encodes the LeuDH protein that has a wide of range of substrates including leucine, isoleucine, valine, and 2-aminobutanoate (Stoyan et al., J. Biotechnol 54:77-80 (1997); Ansorge et al., Biotechnol Bioeng. 68:557-562 (2000)). The nadX gene from Thermotoga maritime encoding for the aspartate dehydrogenase is involved in the biosynthesis of NAD (Yang et al., J. Biol. Chem. 278:8804-8808 (2003)).
(209) TABLE-US-00012 Gene name Gl # Accession # Organism gdhA 118547 P00370 Escherichia coli gdh 6226595 P96110.4 Thermotoga maritima gdhA1 15789827 NP_279651.1 Halobacterium salinarum ldh 61222614 P0A393 Bacillus cereus nadX 15644391 NP_229443.1 Thermotoga maritima
(210) The lysine 6-dehydrogenase (deaminating), encoded by the lysDH genes, catalyze the oxidative deamination of the ε-amino group of L-lysine to form 2-aminoadipate-6-semialdehyde, which in turn nonenzymatically cyclizes to form Δ.sup.1-piperideine-6-carboxylate (Misono et al., J. Bacteriol. 150:398-401 (1982)). Exemplary enzymes can be found in Geobacillus stearothermophilus (Heydari et al., Appl Environ. Microbiol 70:937-942 (2004)), Agrobacterium tumefaciens (Hashimoto et al., J Biochem 106:76-80 (1989); Misono et al., supra), and Achromobacter denitrificans (Ruldeekulthamrong et al., BMB. Rep. 41:790-795 (2008)). Such enzymes are particularly good candidates for converting adipate semialdehyde to 6-aminocaproate given the structural similarity between adipate semialdehyde and 2-aminoadipate-6-semialdehyde.
(211) TABLE-US-00013 Gene GenBank name Gl # Accession # Organism lysDH 13429872 BAB39707 Geobacillus stearothermophilus lysDH 15888285 NP_353966 Agrobacterium tumefaciens lysDH 74026644 AAZ94428 Achromobacter denitrificans
(212) 2.3.1.b Acyl transferase. Referring to
(213) TABLE-US-00014 Gene GenBank name Gl # Accession # Organism paaJ 16129358 NP_415915.1 Escherichia coli pcaF 17736947 AAL02407 Pseudomonas knackmussii (B13) phaD 3253200 AAC24332.1 Pseudomonas putida paaE 106636097 ABF82237.1 Pseudomonas fluorescens
(214) 2-Amino-4-oxopentanoate (AKP) thiolase or AKP thiolase (AKPT) enzymes present additional candidates for performing step A in
(215) TABLE-US-00015 Gene name Gl # GenBank Accession # Organism ortA (α) 126698017 YP_001086914.1 Clostridium difficile 630 ortB (β) 126698018 YP_001086915.1 Clostridium difficile 630 Amet_2368 (α) 150390132 YP_001320181.1 Alkaliphilus metalliredigenes QYF Amet_2369 (β) 150390133 YP_001320182.1 Alkaliphilus metalliredigenes QYF Teth514_1478 (α) 167040116 YP_001663101.1 Thermoanaerobacter sp. X514 Teth514_1479 (β) 167040117 YP_001663102.1 Thermoanaerobacter sp. X514 TTE1235 (α) 20807687 NP_622858.1 Thermoanaerobacter tengcongensis MB4 thrC (β) 20807688 NP_622859.1 Thermoanaerobacter tengcongensis MB4
(216) 2.6.1.a Aminotransferase. Step O of
(217) TABLE-US-00016 Gene GenBank name Gl # Accession # Organism gabT 16130576 NP_417148.1 Escherichia coli puuE 16129263 NP_415818.1 Escherichia coli abat 37202121 NP_766549.2 Mus musculus gabT 70733692 YP_257332.1 Pseudomonas fluorescens abat 47523600 NP_999428.1 Sus scrofa
(218) Additional enzyme candidates include putrescine aminotransferases or other diamine aminotransferases. Such enzymes are particularly well suited for carrying out the conversion of 6-aminocaproate semialdehyde to hexamethylenediamine. The E. coli putrescine aminotransferase is encoded by the ygjG gene and the purified enzyme also was able to transaminate cadaverine and spermidine (Samsonova et al., BMC Microbiol 3:2 (2003)). In addition, activity of this enzyme on 1,7-diaminoheptane and with amino acceptors other than 2-oxoglutarate (e.g., pyruvate, 2-oxobutanoate) has been reported (Samsonova et al., supra; Kim, K. H., J Biol Chem 239:783-786 (1964)). A putrescine aminotransferase with higher activity with pyruvate as the amino acceptor than alpha-ketoglutarate is the spuC gene of Pseudomonas aeruginosa (Lu et al., J Bacteriol 184:3765-3773 (2002)).
(219) TABLE-US-00017 Gene Gen Bank name Gl # Accession # Organism ygjG 145698310 NP_417544 Escherichia coli spuC 9946143 AAG03688 Pseudomonas aeruginosa
1. Yet additional candidate enzymes include beta-alanine/alpha-ketoglutarate aminotransferases which produce malonate semialdehyde from beta-alanine (WO08027742). The gene product of SkPYD4 in Saccharomyces kluyveri was also shown to preferentially use beta-alanine as the amino group donor (Andersen et al., FEBS. J. 274:1804-1817 (2007)). SkUGA1 encodes a homologue of Saccharomyces cerevisiae GABA aminotransferase, UGA1 (Ramos et al., Eur. J. Biochem., 149:401-404 (1985)), whereas SkPYD4 encodes an enzyme involved in both β-alanine and GABA transamination (Andersen et al., supra). 3-Amino-2-methylpropionate transaminase catalyzes the transformation from methylmalonate semialdehyde to 3-amino-2-methylpropionate. This enzyme has been characterized in Rattus norvegicus and Sus scrofa and is encoded by Abat (Tamaki et al, Methods Enzymol, 324:376-389 (2000)).
(220) TABLE-US-00018 Gene GenBank name Gl # Accession # Organism SkyPYD4 98626772 ABF58893.1 Saccharomyces kluyveri SkUGA1 98626792 ABF58894.1 Saccharomyces kluyveri UGA1 6321456 NP_011533.1 Saccharomyces cerevisiae Abat 122065191 P50554.3 Rattus norvegicus Abat 120968 P80147.2 Sus scrofa
(221) 2.8.3.a Coenzyme-A transferase. CoA transferases catalyze reversible reactions that involve the transfer of a CoA moiety from one molecule to another. For example, step E of
(222) TABLE-US-00019 GeneBank Gene name Gl # Accession # Organism pcal 24985644 AAN69545.1 Pseudomonas putida pcaJ 26990657 NP_746082.1 Pseudomonas putida pcal 50084858 YP_046368.1 Acinetobacter sp. ADP1 pcaJ 141776 AAC37147.1 Acinetobacter sp. ADP1 pcal 21224997 NP_630776.1 Streptomyces coelicolor pcaJ 21224996 NP_630775.1 Streptomyces coelicolor HPAG1_0676 108563101 YP_627417 Helicobacter pylori HPAG1_0677 108563102 YP_627418 Helicobacter pylori ScoA 16080950 NP_391778 Bacillus subtilis ScoB 16080949 NP_391777 Bacillus subtilis
(223) A 3-oxoacyl-CoA transferase that can utilize acetate as the CoA acceptor is acetoacetyl-CoA transferase, encoded by the E. coli atoA (alpha subunit) and atoD (beta subunit) genes (Vanderwinkel et al., Biochem. Biophys. Res Commun. 33:902-908 (1968); Korolev et al., Acta Crystallogr. D Biol Crystallogr. 58:2116-2121 (2002)). This enzyme has also been shown to transfer the CoA moiety to acetate from a variety of branched and linear acyl-CoA substrates, including isobutyrate (Matthies et al., Appl Environ Microbiol 58:1435-1439 (1992)), valerate (Vanderwinkel et al., supra) and butanoate (Vanderwinkel et al., supra). Similar enzymes exist in Corynebacterium glutamicum ATCC 13032 (Duncan et al., Appl Environ Microbiol 68:5186-5190 (2002)), Clostridium acetobutylicum (Cary et al., Appl Environ Microbiol 56:1576-1583 (1990)), and Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol Biochem. 71:58-68 (2007)).
(224) TABLE-US-00020 Gene name Gl # Accession # Organism atoA 2492994 P76459.1 Escherichia coli K12 atoD 2492990 P76458.1 Escherichia coli K12 actA 62391407 YP _226809.1 Corynebacterium glutamicum ATCC 13032 cg0592 62389399 YP_224801.1 Corynebacterium glutamicum ATCC 13032 ctfA 15004866 NP_149326.1 Clostridium acetobutylicum ctfB 15004867 NP_149327.1 Clostridium acetobutylicum ctfA 31075384 AAP42564.1 Clostridium saccharoperbutylacetonicum ctfB 31075385 AAP42565.1 Clostridium saccharoperbutylacetonicum
(225) The above enzymes may also exhibit the desired activities on adipyl-CoA and adipate (
(226) TABLE-US-00021 Gene GeneBank name Gl # Accession # Organism cat1 729048 P38946.1 Clostridium kluyveri cat2 172046066 P38942.2 Clostridium kluyveri cat3 146349050 EDK35586.1 Clostridium kluyveri
(227) The glutaconate-CoA-transferase (EC 2.8.3.12) enzyme from anaerobic bacterium Acidaminococcus fermentans reacts with diacid glutaconyl-CoA and 3-butenoyl-CoA (Mack et al., FEBS Lett. 405:209-212 (1997)). The genes encoding this enzyme are gctA and gctB. This enzyme has reduced but detectable activity with other CoA derivatives including glutaryl-CoA, 2-hydroxyglutaryl-CoA, adipyl-CoA and acrylyl-CoA (Buckel et al., Eur. J. Biochem. 118:315-321 (1981)). The enzyme has been cloned and expressed in E. coli (Mack et al., Eur. J. Biochem. 226:41-51 (1994)).
(228) TABLE-US-00022 Gene GeneBank name Gl # Accession # Organism gctA 559392 CAA57199.1 Acidaminococcus fermentans gctB 559393 CAA57200.1 Acidaminococcus fermentans
(229) 3.1.2.a Thiolester hydrolase (CoA specific). Several eukaryotic acetyl-CoA hydrolases have broad substrate specificity and thus represent suitable candidate enzymes for hydrolyzing 3-oxoadipyl-CoA, adipyl-CoA, 3-oxo-6-aminohexanoyl-CoA, or 6-aminocaproyl-CoA (Steps G and M of
(230) TABLE-US-00023 Gene GenBank name Gl # Accession # Organism acot12 18543355 NP_570103.1 Rattus norvegicus
(231) Additional hydrolase enzymes include 3-hydroxyisobutyryl-CoA hydrolase which has been described to efficiently catalyze the conversion of 3-hydroxyisobutyryl-CoA to 3-hydroxyisobutyrate during valine degradation (Shimomura et al., J Biol Chem. 269:14248-14253 (1994)). Genes encoding this enzyme include hibch of Rattus norvegicus (Shimomura et al., supra; Shimomura et al., Methods Enzymol. 324:229-240 (2000)) and Homo sapiens (Shimomura et al., supra). Candidate genes by sequence homology include hibch of Saccharomyces cerevisiae and BC 2292 of Bacillus cereus.
(232) TABLE-US-00024 Gene Gen Bank name Gl # Accession # Organism hibch 146324906 Q5XIE6.2 Rattus norvegicus hibch 146324905 Q6NVY1.2 Homo sapiens hibch 2506374 P28817.2 Saccharomyces cerevisiae BC_2292 29895975 AP09256 Bacillus cereus
(233) Yet another candidate hydrolase is the human dicarboxylic acid thioesterase, acot8, which exhibits activity on glutaryl-CoA, adipyl-CoA, suberyl-CoA, sebacyl-CoA, and dodecanedioyl-CoA (Westin et al., J. Biol. Chem. 280:38125-38132 (2005)) and the closest E. coli homolog, tesB, which can also hydrolyze a broad range of CoA thiolesters (Naggert et al., J Biol Chem 266:11044-11050 (1991)). A similar enzyme has also been characterized in the rat liver (Deana R., Biochem Int 26:767-773 (1992)).
(234) TABLE-US-00025 Gene Gen Bank name Gl # Accession # Organism tesB 16128437 NP_414986 Escherichia coli acot8 3191970 CAA15502 Homo sapiens acot8 51036669 NP_570112 Rattus norvegicus
(235) Other potential E. coli thiolester hydrolases include the gene products of tesA (Bonner et al., J Biol Chem 247:3123-3133 (1972)), ybgC (Kuznetsova et al., FEMS Microbiol Rev 29:263-279 (2005); Zhuang et al., FEBS Lett 516:161-163 (2002)), paaI (Song et al., J Biol Chem 281:11028-11038 (2006)), and ybdB (Leduc et al., J Bacteriol 189:7112-7126 (2007)).
(236) TABLE-US-00026 Gene Gen Bank name Gl # Accession # Organism tesA 16128478 NP_415027 Escherichia coli ybgC 16128711 NP_415264 Escherichia coli paal 16129357 NP_415914 Escherichia coli ybdB 16128580 NP_415129 Escherichia coli
(237) 6.3.1.a/6.3.2.a amide synthases/peptide synthases. The direct conversion of 6-aminocaproate to caprolactam (Step S,
(238) TABLE-US-00027 GenBank Gene name GI# Accession # Organism acsA 60650089 BAD90933 Pseudomonas chlororaphis puuA 87081870 AAC74379 Escherichia coli bls 41016784 Q9R8E3 Streptomyces clavuligerus
(239) 4.2.1.a Hydrolyase. Most dehydratases catalyze the α,β-elimination of water. This involves activation of the α-hydrogen by an electron-withdrawing carbonyl, carboxylate, or CoA-thiol ester group and removal of the hydroxyl group from the β-position. Enzymes exhibiting activity on substrates with an electron-withdrawing carboxylate group are excellent candidates for dehydrating 3-hydroxyadipate (
(240) For example, fumarase enzymes naturally catalyze the reversible dehydration of malate to fumarate. E. coli has three fumarases: FumA, FumB, and FumC that are regulated by growth conditions. FumB is oxygen sensitive and only active under anaerobic conditions. FumA is active under microanaerobic conditions, and FumC is the only active enzyme in aerobic growth (Tseng et al., J Bacteriol 183:461-467 (2001); Woods et al., Biochim Biophys Acta 954:14-26 (1988); Guest et al., J Gen Microbiol 131:2971-2984 (1985)). Additional enzyme candidates are found in Campylobacter jejuni (Smith et al., Int. J Biochem. Cell Biol 31:961-975 (1999)), Thermus thermophilus (Mizobata et al., Arch. Biochem. Biophys. 355:49-55 (1998)) and Rattus norvegicus (Kobayashi et al., J Biochem. 89:1923-1931 (1981)). Similar enzymes with high sequence homology include fumI from Arabidopsis thaliana and fumC from Corynebacterium glutamicum. The MmcBC fumarase from Pelotomaculum thermopropionicum is another class of fumarase with two subunits (Shimovama et al., FEMS Microbiol Lett 270:207-213 (2007)).
(241) TABLE-US-00028 GenBank Gene name GI# Accession # Organism fumA 81175318 P0AC33 Escherichia coli fumB 33112655 P14407 Escherichia coli fumC 120601 P05042 Escherichia coli fumC 9789756 O69294 Campylobacter jejuni fumC 3062847 BAA25700 Thermus thermophilus fumH 120605 P14408 Rattus norvegicus fum1 39931311 P93033 Arabidopsis thaliana fumC 39931596 Q8NRN8 Corynebacterium glutamicum MmcB 147677691 YP_001211906 Pelotomaculum thermopropionicum MmcC 147677692 YP_001211907 Pelotomaculum thermopropionicum
(242) Two additional dehydratase candidates are 2-(hydroxymethyl)glutarate dehydratase and dimethylmaleate hydratase, enzymes studied for their role in nicontinate catabolism in Eubacterium barkeri (formerly Clostridium barkeri) (Alhapel et al., Proc Natl Acad Sci USA 103:12341-6 (2006)). 2-(Hydroxymethyl)glutarate dehydratase is a [4Fe-4S]-containing enzyme that dehydrates 2-(hydroxymethyl)glutarate to 2-methylene-glutarate. This enzyme is encoded by hmd in Eubacterium barkeri (Alhapel et al., supra). Similar enzymes with high sequence homology are found in Bacteroides capillosus, Anaerotruncus colihominis, and Natranaerobius thermophilius. These enzymes are homologous to the alpha and beta subunits of [4Fe-45]-containing bacterial serine dehydratases (e.g., E. coli enzymes encoded by tdcG, sdhB, and sdaA).
(243) TABLE-US-00029 GenBank Gene name GI# Accession # Organism hmd 86278275 ABC88407.1 Eubacterium barkeri BACCAP_02294 154498305 ZP_02036683.1 Bacteroides capillosus ANACOL_02527 167771169 ZP_02443222.1 Anaerotruncus colihominis DSM 17241 NtherDRAFT_2368 169192667 ZP_02852366.1 Natranaerobius thermophilus JW/NM-WN-LF
(244) Dimethylmaleate hydratase (EC 4.2.1.85) is a reversible Fe.sup.2+-dependent and oxygen-sensitive enzyme in the aconitase family that hydrates dimethylmaeate to form (2R,3S)-2,3-dimethylmalate. This enzyme is encoded by dmdAB in Eubacterium barkeri (Alhapel et al., supra; Kollmann-Koch et al., Hoppe Seylers. Z. Physiol Chem. 365:847-857 (1984)).
(245) TABLE-US-00030 GenBank Gene name GI# Accession # Organism dmdA 86278276 ABC88408 Eubacterium barkeri dmdB 86278277 ABC88409.1 Eubacterium barkeri
(246) An additional enzyme candidate is 2-methylmalate dehydratase, also called citramalate hydrolyase, a reversible hydrolyase that catalyzes the alpha, beta elimination of water from citramalate to form mesaconate. This enzyme has been purified and characterized in Clostridium tetanomorphum (Wang et al., J. Biol. Chem. 244:2516-2526 (1969)). The activity of this enzyme has also been detected in several bacteria in the genera Citrobacter and Morganella in the context of the glutamate degradation VI pathway (Kato et al., Arch. Microbiol 168:457-463 (1997)). Genes encoding this enzyme have not been identified in any organism to date.
(247) Enzymes exhibiting activity on substrates with an electron-withdrawing CoA-thiol ester group adjacent to the α-hydrogen are excellent candidates for dehydrating 3-hydroxyadipyl-CoA (
(248) TABLE-US-00031 GenBank Gene name GI# Accession # Organism paaA 26990002 NP_745427.1 Pseudomonas fluorescens paaB 26990001 NP_745426.1 Pseudomonas fluorescens phaA 106636093 ABF82233.1 Pseudomonas putida phaB 106636094 ABF82234.1 Pseudomonas putida maoC 16129348 NP_415905.1 Escherichia coli paaF 16129354 NP_415911.1 Escherichia coli paaG 16129355 NP_415912.1 Escherichia coli crt 15895969 NP_349318.1 Clostridium acetobutylicum crt1 153953091 YP_001393856 Clostridium kluyveri DSM 555
(249) 6.2.1.a Acid-thiol ligase. Steps F, L, and R of
(250) TABLE-US-00032 GenBank Gene name GI# Accession # Organism sucC 16128703 NP_415256.1 Escherichia coli sucD 1786949 AAC73823.1 Escherichia coli
(251) Additional exemplary CoA-ligases include the rat dicarboxylate-CoA ligase for which the sequence is yet uncharacterized (Vamecq et al., Biochemical Journal 230:683-693 (1985)), either of the two characterized phenylacetate-CoA ligases from P. chrysogenum (Lamas-Maceiras et al., Biochem. J. 395:147-155 (2005); Wang et al., Biochem Biophy Res Commun 360(2):453-458 (2007)), the phenylacetate-CoA ligase from Pseudomonas putida (Martinez-Blanco et al., J. Biol. Chem. 265:7084-7090 (1990)), and the 6-carboxyhexanoate-CoA ligase from Bacillus subtilis (Bower et al., J. Bacteriol. 178(14):4122-4130 (1996)). Additional candidate enzymes are acetoacetyl-CoA synthetases from Mus musculus (Hasegawa et al., Biochim Biophys Acta 1779:414-419 (2008)) and Homo sapiens (Ohgami et al., Biochem Pharmacol 65:989-994 (2003)) which naturally catalyze the ATP-dependent conversion of acetoacetate into acetoacetyl-CoA.
(252) TABLE-US-00033 GenBank Gene name GI# Accession # Organism phl 77019264 CAJ15517.1 Penicillium chrysogenum phlB 152002983 ABS19624.1 Penicillium chrysogenum paaF 22711873 AAC24333.2 Pseudomonas putida bioW 50812281 NP_390902.2 Bacillus subtilis AACS 21313520 NP_084486.1 Mus musculus AACS 31982927 NP_076417.2 Homo sapiens
(253) ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) is another candidate enzyme that couples the conversion of acyl-CoA esters to their corresponding acids with the concurrent synthesis of ATP. Several enzymes with broad substrate specificities have been described in the literature. ACD I from Archaeoglobus fulgidus, encoded by AF1211, was shown to operate on a variety of linear and branched-chain substrates including acetyl-CoA, propionyl-CoA, butyryl-CoA, acetate, propionate, butyrate, isobutyryate, isovalerate, succinate, fumarate, phenylacetate, indoleacetate (Musfeldt et al., J Bacteriol 184:636-644 (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch Microbiol 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen et al., supra). The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Musfeldt et al., supra; Brasen et al., supra).
(254) TABLE-US-00034 GenBank Gene name GI# Accession # Organism AF1211 11498810 NP_070039.1 Archaeoglobus fulgidus DSM 4304 scs 55377722 YP_135572.1 Haloarcula marismortui ATCC 43049 PAE3250 18313937 NP_560604.1 Pyrobaculum aerophilum str. IM2
(255) Yet another option is to employ a set of enzymes with net ligase or synthetase activity. For example, phosphotransadipylase and adipate kinase enzymes are catalyzed by the gene products of buk1, buk2, and ptb from C. acetobutylicum (Walter et al., Gene 134:107-111 (1993); Huang et al., J. Mol. Microbiol. Biotechnol. 2:33-38 (2000)). The ptb gene encodes an enzyme that can convert butyryl-CoA into butyryl-phosphate, which is then converted to butyrate via either of the buk gene products with the concomitant generation of ATP.
(256) TABLE-US-00035 GenBank Gene name GI# Accession # Organism ptb 15896327 NP_349676 Clostridium acetobutylicum buk1 15896326 NP_349675 Clostridium acetobutylicum buk2 20137415 Q97II1 Clostridium acetobutylicum
(257) No enzyme required—Spontaneous cyclization. 6-Aminocaproyl-CoA will cyclize spontaneously to caprolactam, thus eliminating the need for a dedicated enzyme for this step. A similar spontaneous cyclization is observed with 4-aminobutyryl-CoA which forms pyrrolidinone (Ohsugi et al., J Biol Chem 256:7642-7651 (1981)).
(258) Microbial organisms may also be generated that are capable of producing hexamethylenediamine from acetyl-CoA and succinyl-CoA and as shown in
(259) In other examples, hexamethylenediamine can be produced via a pathway for converting acetyl-CoA and 4-aminobutyryl-CoA to 6-Aminocaproyl-CoA
(260) Another pathway to produce hexamethylenediamine is from acetyl-CoA and 4-aminobutyryl-CoA.
(261) The paaJ (NP-415915.1), paaH (NP-415913.1), and maoC (NP-415905.1) genes encoding the 3-oxo-6-aminohexanoyl-CoA thiolase, 3-oxo-6-aminohexanoyl-CoA reductase, 3-hydroxy-6-aminohexanoyl-CoA dehydratase activities, respectively, can be cloned into the pZE13 vector (Expressys, Ruelzheim, Germany) under the PA1/lacO promoter. In addition, the bcd (NP-349317.1), etfAB (NP-349315.1 and NP-349316.1), acrI (YP-047869.1), and ygjG (NP-417544) genes encoding 6-aminohex-2-enoyl-CoA reductase, 6-aminocaproyl-CoA reductase (aldehyde forming), and hexamethylenediamine transaminase activities are cloned into the pZA33 vector (Expressys, Ruelzheim, Germany) under the PA1/lacO promoter. Lastly, the sucD (NP-904963.1), gabT (NP-417148.1), and cat2 (P38942.2) genes encoding succinyl-CoA reductase (aldehyde forming), GABA transaminase, and 4-aminobutyryl-CoA/acyl-CoA transferase activities are cloned into a third compatible plasmid, pZS23, under the PA1/lacO promoter, to increase the availability of 4-aminobutyryl-CoA. pZS23 is obtained by replacing the ampicillin resistance module of the pZS13 vector (Expressys, Ruelzheim, Germany) with a kanamycin resistance module by well-known molecular biology techniques. The three sets of plasmids are transformed into E. coli strain MG1655 to express the proteins and enzymes required for hexamethylenediamine synthesis.
(262) Hexamethylenediamine may also be produced from 6-Aminocaproate (6-ACA). This pathway involves activation of the acid group by phosphorylation and/or acylation. Acetylation of the terminal amino group provides protection from spontaneous cyclization of pathway intermediates.
(263) Several pathways for producing HMD from 6-aminocaproate are detailed in
(264) In one route, 6-aminocaproate is phosphorylated to 6-AHOP by 6-aminocaproate kinase (
(265) Alternately, 6-AHOP is converted to 6-aminocaproyl-CoA by an acyltransferase (
(266) In yet another route, 6-aminocaproate is first activated to a CoA derivative by a CoA transferase or CoA ligase (
(267) Additional routes proceed from 6-acetamidohexanoate, the acetylated product of 6-aminocaproate N-acetyltransferase. 6-Acetamidohexanoate is converted to 6-acetamidohexanal by different routes (described below). In the final two steps of these routes, 6-acetamidohexanal is first converted to 6-acetamidohexanamine by an aminotransferase or an aminating oxidoreductase (
(268) In one route, 6-acetamidohexanoate is phosphorylated by 6-acetamidohexanoate kinase (
(269) In another route, 6-acetamidohexanoate is activated to 6-acetamidohexanoyl-CoA by a CoA transferase or CoA ligase (
(270) Alternately, 6-acetamidohexanoate is phosphorylated to 6-AAHOP (
(271) The transformations depicted in
(272) Table 9 shows the enzyme types useful to convert common central metabolic intermediates into 6-aminocaproate and hexamethylenediamine. The first three digits of each label correspond to the first three Enzyme Commission number digits which denote the general type of transformation independent of substrate specificity.
(273) TABLE-US-00036 TABLE 9 LABEL FUNCTION 1.2.1.b Oxidoreductase (acyl-CoA to aldehyde) 1.2.1.c Oxidoreductase (2-ketoacid to acyl-CoA) 1.2.1.d Oxidoreductase (phosphonic acid to aldehyde) 1.3.1.a Oxidoreductase (alkene to alkane) 1.4.1.a Oxidoreductase (ketone or aldehyde to amino) 2.3.1.a Acyltransferase (transferring CoA to phospho) 2.3.1.c Acyltransferase (N-acetyltransferase) 2.3.1.d Acyltransferase (formate C-acyltransferase) 2.6.1.a Aminotransferase 2.7.2.a Phosphotransferase (carboxy acceptor) 2.8.3.a Coenzyme-A transferase 3.5.1.a Hydrolase (acting on linear amides) 4.1.1.a Carboxy-lyase 4.1.2.a Aldehyde-lyase 4.2.1.a Hydro-lyase 6.2.1.a Acid-thiol ligase
(274) 1.2.1.b Oxidoreductase (acyl-CoA to aldehyde). The transformations of 6-acetamidohexanoyl-CoA to 6-acetamidohexanal (
(275) TABLE-US-00037 GenBank Gene name GI# Accession # Organism acr1 50086359 YP_047869.1 Acinetobacter calcoaceticus acr1 1684886 AAC45217 Acinetobacter baylyi acr1 18857901 BAB85476.1 Acinetobacter sp. Strain M-1 sucD 172046062 P38947.1 Clostridium kluyveri sucD 34540484 NP_904963.1 Porphyromonas gingivalis bphG 425213 BAA03892.1 Pseudomonas sp adhE 55818563 AAV66076.1 Leuconostoc mesenteroides
(276) An additional enzyme that converts an acyl-CoA to its corresponding aldehyde is malonyl-CoA reductase which transforms malonyl-CoA to malonic semialdehyde. Malonyl-CoA reductase is a key enzyme in autotrophic carbon fixation via the 3-hydroxypropionate cycle in thermoacidophilic archaeal bacteria (Berg et al., Science 318:1782-1786 (2007); and Thauer, R. K., Science. 318:1732-1733 (2007)). The enzyme utilizes NADPH as a cofactor and has been characterized in Metallosphaera and Sulfolobus sp (Alber et al., J. Bacteriol. 188:8551-8559 (2006); and Hugler et al., J. Bacteriol. 184:2404-2410 (2002)). The enzyme is encoded by Msed_0709 in Metallosphaera sedula (Alber et al., J. Bacteriol. 188:8551-8559 (2006); and Berg et al., Science. 318:1782-1786 (2007)). A gene encoding a malonyl-CoA reductase from Sulfolobus tokodaii was cloned and heterologously expressed in E. coli (Alber et al., J. Bacteriol. 188:8551-8559 (2006)). This enzyme has also been shown to catalyze the conversion of methylmalonyl-CoA to its corresponding aldehyde (WIPO Patent Application WO/2007/141208 Kind Code: A2). Although the aldehyde dehydrogenase functionality of these enzymes is similar to the bifunctional dehydrogenase from Chloroflexus aurantiacus, there is little sequence similarity. Both malonyl-CoA reductase enzyme candidates have high sequence similarity to aspartate-semialdehyde dehydrogenase, an enzyme catalyzing the reduction and concurrent dephosphorylation of aspartyl-4-phosphate to aspartate semialdehyde. Additional gene candidates can be found by sequence homology to proteins in other organisms including Sulfolobus solfataricus and Sulfolobus acidocaldarius and have been listed below. Yet another candidate for CoA-acylating aldehyde dehydrogenase is the ald gene from Clostridium beijerinckii (Toth et al., Appl Environ Microbiol 65:4973-4980 (1999)). This enzyme has been reported to reduce acetyl-CoA and butyryl-CoA to their corresponding aldehydes. This gene is very similar to eutE that encodes acetaldehyde dehydrogenase of Salmonella typhimurium and E. coli (Toth et al., Appl Environ Microbiol 65:4973-4980 (1999)).
(277) TABLE-US-00038 GenBank Gene name GI# Accession # Organism Msed_0709 146303492 YP_001190808.1 Metallosphaera sedula mcr 15922498 NP_378167.1 Sulfolobus tokodaii asd-2 15898958 NP_343563.1 Sulfolobus solfataricus Saci_2370 70608071 YP_256941.1 Sulfolobus acidocaldarius Ald 49473535 AAT66436 Clostridium beijerinckii eutE 687645 AAA80209 Salmonella typhimurium eutE 2498347 P77445 Escherichia coli
(278) 1.2.1.c Oxidoreductase (2-ketoacid to acyl-CoA). Several transformations in
(279) Activity of enzymes in the 2-keto-acid dehydrogenase family is normally low or limited under anaerobic conditions in E. coli. Increased production of NADH (or NADPH) could lead to a redox-imbalance, and NADH itself serves as an inhibitor to enzyme function. Engineering efforts have increased the anaerobic activity of the E. coli pyruvate dehydrogenase complex (Kim et al., Appl. Environ. Microbiol. 73:1766-1771 (2007); Kim et al., J. Bacteriol. 190:3851-3858 (2008); and Zhou et al., Biotechnol. Lett. 30:335-342 (2008)). For example, the inhibitory effect of NADH can be overcome by engineering an H322Y mutation in the E3 component (Kim et al., J. Bacteriol. 190:3851-3858 (2008)). Structural studies of individual components and how they work together in complex provide insight into the catalytic mechanisms and architecture of enzymes in this family (Aevarsson et al., Nat. Struct. Biol. 6:785-792 (1999); and Zhou et al., Proc. Natl. Acad. Sci. U.S. A 98:14802-14807 (2001)). The substrate specificity of the dehydrogenase complexes varies in different organisms, but generally branched-chain keto-acid dehydrogenases have the broadest substrate range.
(280) Alpha-ketoglutarate dehydrogenase (AKGD) converts alpha-ketoglutarate to succinyl-CoA and is the primary site of control of metabolic flux through the TCA cycle (Hansford, Curr. Top. Bioenerg. 10:217-278 (1980)). Encoded by genes sucA, sucB and lpd in E. coli, AKGD gene expression is downregulated under anaerobic conditions and during growth on glucose (Park et al., Mol. Microbiol. 15:473-482 (1995)). Although the substrate range of AKGD is narrow, structural studies of the catalytic core of the E2 component pinpoint specific residues responsible for substrate specificity (Knapp et al., J. Mol. Biol. 280:655-668 (1998)). The Bacillus subtilis AKGD, encoded by odhAB (E1 and E2) and pdhD (E3, shared domain), is regulated at the transcriptional level and is dependent on the carbon source and growth phase of the organism (Resnekov et al., Mol. Gen. Genet. 234:285-296 (1992)). In yeast, the LPD1 gene encoding the E3 component is regulated at the transcriptional level by glucose (Roy and Dawes, J. Gen. Microbiol. 133:925-933 (1987)). The E1 component, encoded by KGD1, is also regulated by glucose and activated by the products of HAP2 and HAP3 (Repetto and Tzagoloff, Mol. Cell. Biol. 9:2695-2705 (1989)). The AKGD enzyme complex, inhibited by products NADH and succinyl-CoA, is well-studied in mammalian systems, as impaired function of has been linked to several neurological diseases (Tretter and dam-Vizi, Philos. Trans. R. Soc. Lond B Biol. Sci. 360:2335-2345 (2005)).
(281) TABLE-US-00039 GenBank Gene name GI# Accession # Organism sucA 16128701 NP_415254.1 Escherichia coli sucB 16128702 NP_415255.1 Escherichia coli lpd 16128109 NP_414658.1 Escherichia coli odhA 51704265 P23129.2 Bacillus subtilis odhB 129041 P16263.1 Bacillus subtilis pdhD 118672 P21880.1 Bacillus subtilis KGD1 6322066 NP_012141.1 Saccharomyces cerevisiae KGD2 6320352 NP_010432.1 Saccharomyces cerevisiae LPD1 14318501 NP_116635.1 Saccharomyces cerevisiae
(282) Branched-chain 2-keto-acid dehydrogenase complex (BCKAD), also known as 2-oxoisovalerate dehydrogenase, participates in branched-chain amino acid degradation pathways, converting 2-keto acids derivatives of valine, leucine and isoleucine to their acyl-CoA derivatives and CO.sub.2. The complex has been studied in many organisms including Bacillus subtilis (Wang et al., Eur. J. Biochem. 213:1091-1099 (1993)), Rattus norvegicus (Namba et al., J. Biol. Chem. 244:4437-4447 (1969)) and Pseudomonas putida (Sokatch et al., J. Bacteriol. 148:647-652 (1981)). In Bacillus subtilis the enzyme is encoded by genes pdhD (E3 component), bfmBB (E2 component), bfmBAA and bfmBAB (E1 component) (Wang et al., Eur. J. Biochem. 213:1091-1099 (1993)). In mammals, the complex is regulated by phosphorylation by specific phosphatases and protein kinases. The complex has been studied in rat hepatocites (Chicco et al., J. Biol. Chem. 269:19427-19434 (1994)) and is encoded by genes Bckdha (E1 alpha), Bckdhb (E1 beta), Dbt (E2), and Dld (E3). The E1 and E3 components of the Pseudomonas putida BCKAD complex have been crystallized (Aevarsson et al., Nat. Struct. Biol. 6:785-792 (1999); and Mattevi et al., Science. 255:1544-1550 (1992)) and the enzyme complex has been studied (Sokatch et al., J. Bacteriol. 148:647-652 (1981)). Transcription of the P. putida BCKAD genes is activated by the gene product of bkdR (Hesslinger et al., Mol. Microbiol. 27:477-492 (1998)). In some organisms including Rattus norvegicus (Paxton et al., Biochem. J. 234:295-303 (1986)) and Saccharomyces cerevisiae (Sinclair et al., Biochem. Mol. Biol. Int. 31: 911-922 (1993)), this complex has been shown to have a broad substrate range that includes linear oxo-acids such as 2-oxobutanoate and alpha-ketoglutarate, in addition to the branched-chain amino acid precursors. The active site of the bovine BCKAD was engineered to favor alternate substrate acetyl-CoA (Meng and Chuang, Biochemistry. 33:12879-12885 (1994)).
(283) TABLE-US-00040 GenBank Gene name GI# Accession # Organism bfmBB 16079459 NP_390283.1 Bacillus subtilis bfmBAA 16079461 NP_390285.1 Bacillus subtilis bfmBAB 16079460 NP_390284.1 Bacillus subtilis pdhD 118672 P21880.1 Bacillus subtilis lpdV 118677 P09063.1 Pseudomonas putida bkdB 129044 P09062.1 Pseudomonas putida bkdA1 26991090 NP_746515.1 Pseudomonas putida bkdA2 26991091 NP_746516.1 Pseudomonas putida Bckdha 77736548 NP_036914.1 Rattus norvegicus Bckdhb 158749538 NP_062140.1 Rattus norvegicus Dbt 158749632 NP_445764.1 Rattus norvegicus Dld 40786469 NP_955417.1 Rattus norvegicus
(284) The pyruvate dehydrogenase complex, catalyzing the conversion of pyruvate to acetyl-CoA, has also been extensively studied. In the E. coli enzyme, specific residues in the E1 component are responsible for substrate specificity (Bisswanger, J Biol Chem. 256:815-822 (1981); Bremer, Eur. J Biochem. 8:535-540 (1969); and Gong et al., J Biol Chem. 275:13645-13653 (2000)). As mentioned previously, enzyme engineering efforts have improved the E. coli PDH enzyme activity under anaerobic conditions (Kim et al., Appl. Environ. Microbiol. 73:1766-1771 (2007); Kim et al., J. Bacteriol. 190:3851-3858 (2008)); and Zhou et al., Biotechnol. Lett. 30:335-342 (2008)). In contrast to the E. coli PDH, the B. subtilis complex is active and required for growth under anaerobic conditions (Nakano et al., J. Bacteriol. 179:6749-6755 (1997)). The Klebsiella pneumoniae PDH, characterized during growth on glycerol, is also active under anaerobic conditions (Menzel et al., J. Biotechnol. 56:135-142 (1997)). Crystal structures of the enzyme complex from bovine kidney (Zhou et al., Proc. Natl. Acad. Sci. U.S. A 98:14802-14807 (2001)) and the E2 catalytic domain from Azotobacter vinelandii are available (Mattevi et al., Science. 255:1544-1550 (1992)). Some mammalian PDH enzymes complexes can react on alternate substrates such as 2-oxobutanoate, although comparative kinetics of Rattus norvegicus PDH and BCKAD indicate that BCKAD has higher activity on 2-oxobutanoate as a substrate (Paxton et al., Biochem. J. 234:295-303 (1986)).
(285) TABLE-US-00041 GenBank Gene name GI# Accession # Organism aceE 16128107 NP_414656.1 Escherichia coli aceF 16128108 NP_414657.1 Escherichia coli lpd 16128109 NP_414658.1 Escherichia coli pdhA 3123238 P21881.1 Bacillus subtilis pdhB 129068 P21882.1 Bacillus subtilis pdhC 129054 P21883.2 Bacillus subtilis pdhD 118672 P21880.1 Bacillus subtilis ace 152968699 YP_001333808.1 Klebsiella pneumonia aceF 152968700 YP_001333809.1 Klebsiella pneumonia lpdA 152968701 YP_001333810.1 Klebsiella pneumonia Pdha1 124430510 NP_001004072.2 Rattus norvegicus Pdha2 16758900 NP_446446.1 Rattus norvegicus Dlat 78365255 NP_112287.1 Rattus norvegicus Dld 40786469 NP_955417.1 Rattus norvegicus
(286) As an alternative to the large multienzyme 2-keto-acid dehydrogenase complexes described above, some anaerobic organisms utilize enzymes in the 2-ketoacid oxidoreductase family (OFOR) to catalyze acylating oxidative decarboxylation of 2-keto-acids. Unlike the dehydrogenase complexes, these enzymes contain iron-sulfur clusters, utilize different cofactors, and use ferredoxin or flavodoxin as electron acceptors in lieu of NAD(P)H. While most enzymes in this family are specific to pyruvate as a substrate (POR) some 2-keto-acid:ferredoxin oxidoreductases have been shown to accept a broad range of 2-ketoacids as substrates including alpha-ketoglutarate and 2-oxobutanoate (Fukuda and Wakagi, Biochim. Biophys. Acta 1597:74-80 (2002); and Zhang et al., J. Biochem. 120:587-599 (1996)). One such enzyme is the OFOR from the thermoacidophilic archaeon Sulfolobus tokodall 7, which contains an alpha and beta subunit encoded by gene ST2300 (Fukuda and Wakagi, Biochim. Biophys. Acta 1597:74-80 (2002); and Zhang et al., J. Biochem. 120:587-599 (1996)). A plasmid-based expression system has been developed for efficiently expressing this protein in E. coli (Fukuda et al., Eur. J. Biochem. 268:5639-5646 (2001)) and residues involved in substrate specificity were determined (Fukuda and Wakagi, Biochim. Biophys. Acta 1597:74-80 (2002)). Two OFORs from Aeropyrum pernix str. K1 have also been recently cloned into E. coli, characterized, and found to react with a broad range of 2-oxoacids (Nishizawa et al., FEBS Lett. 579:2319-2322 (2005)). The gene sequences of these OFOR candidates are available, although they do not have GenBank identifiers assigned to date. There is bioinformatic evidence that similar enzymes are present in all archaea, some anaerobic bacteria and amitochondrial eukarya (Fukuda and Wakagi, Biochim. Biophys. Acta 1597:74-80 (2002)). This class of enzyme is also interesting from an energetic standpoint, as reduced ferredoxin could be used to generate NADH by ferredoxin-NAD reductase (Petitdemange et al., Biochim. Biophys. Acta 421:334-337 (1976)). Also, since most of the enzymes are designed to operate under anaerobic conditions, less enzyme engineering may be required relative to enzymes in the 2-keto-acid dehydrogenase complex family for activity in an anaerobic environment.
(287) TABLE-US-00042 Gene GenBank name GI# Accession # Organism ST2300 15922633 NP_378302.1 Sulfolobus tokodaii 7
(288) 1.2.1.d Oxidoreductase (phosphonic acid to aldehyde). The reduction of a phosphonic acid to its corresponding aldehyde is catalyzed by an oxidoreductase in the EC class 1.2.1. Steps B and F in
(289) TABLE-US-00043 Gene GenBank name GI# Accession # Organism Asd 16131307 NP_417891.1 Escherichia coli Asd 68249223 YP_248335.1 Haemophilus influenzae Asd 1899206 AAB49996 Mycobacterium tuberculosis V02036 15642038 NP_231670 Vibrio cholera Asd 210135348 YP_002301787.1 Heliobacter pylori ARG5,6 6320913 NP_010992.1 Saccharomyces cerevisiae argC 16078184 NP_389001.1 Bacillus subtilis
(290) 1.3.1.a Oxidoreductase (alkene to alkane). Several transformations fall into the category of oxidoreductases that reduce an alkene to an alkane (EC 1.3.1.-). For example, Steps C, G, K and N in
(291) Enzymes with enone reductase activity have been identified in prokaryotes, eukaryotes and plants (Shimoda et al., Bulletin of the chemical Society of Japan 77:2269-2 (2004); and Wanner and Tressl, Eur. J Biochem. 255:271-278 (1998)). Two enone reductases from the cytosolic fraction of Saccharomyces cerevisiae were purified and characterized, and found to accept a variety of alkenals (similar to 6-OHE) and enoyl ketones (similar to OHED) as substrates (Wanner and Tressl, Eur. J Biochem. 255:271-278 (1998)). Genes encoding these enzymes have not been identified to date. Cell extracts of cyanobacterium Synechococcus sp. PCC7942 reduced a variety enone substrates to their corresponding alkyl ketones (Shimoda et al., Bulletin of the chemical Society of Japan 77:2269-2 (2004)). Genes have not been associated with this activity in this organism. Enone reductases in other organisms can also catalyze this transformation.
(292) A recombinant NADPH-dependent enone reductase from Nicotiana tabacum, encoded by NtRed1, was functionally expressed and characterized in E. coli (Matsushima et al., Bioorganic Chemistry 36:23-28 (2008)). This reductase was functional on the exocyclic enoyl ketone pulegone (Matsushima et al., Bioorganic Chemistry 36:23-28 (2008)). An enzyme candidate in S. cerevisiae at the locus YML131W, bears 30% identity to NtRed1(evalue=1e-26). The amino acid sequence of NtRed1 shares significant homology with 2-alkenal reductase from Arabidopsis thaliana, zeta-crystallin homolog from A. thaliana, pulegone reductase from Menthe piperita and phenylpropenal alkene reductase from Pinus taeda. These enzymes are known to catalyze the reduction of alkenes of α,β-unsaturated ketones and aldehydes.
(293) TABLE-US-00044 Gene GenBank name GI# Accession # Organism NtRed1 6692816 BAA89423 Nicotiana tabacum YML131W 45269874 AAS56318.1 Saccharomyces cerevisiae AtDBR1 15237888 NP-197199 Arabidopsis thaliana P2 886430 CAA89262 Arabidopsis thaliana PulR 34559418 AAQ75423 Menthe piperita PtPPDBR 110816011 ABG91753 Pinus taeda
(294) 2-Alkenal reductase catalyzes the reduction of α,β-unsaturated double bonds of aldehydes and ketones. A barley alkenal hydrogenase ALH1 was identified with activity for a range of α,β-unsaturated ketones and aldehydes including trans-2-nonenal, 2-hexenal, traumatin and 1-octene-3-one (Hambraeus and Nyberg, J Agric. Food Chem. 53:8714-8721 (2005)). The Hordeum vulgare ALH1 cDNA was cloned expressed in E. coli (Hambraeus and Nyberg, J Agric. Food Chem. 53:8714-8721 (2005)).
(295) TABLE-US-00045 Gene GenBank name GI# Accession # Organism ALH1 62765876 AAX99161 Hordeum vulgare ALH1 195652571 ACG45753 Zea mays
(296) 2-Enoate reductase enzymes are known to catalyze the NAD(P)H-dependent reduction of a wide variety of α,β-unsaturated carboxylic acids and aldehydes (Rohdich et al., J. Biol. Chem. 276:5779-5787 (2001)). In the recently published genome sequence of C. kluyveri, 9 coding sequences for enoate reductases were reported, out of which one has been characterized (Seedorf et al., Proc. Natl. Acad. Sci U.S. A 105:2128-2133 (2008)). The enr genes from both C. tyrobutyricum and M. thermoaceticum have been cloned and sequenced and show 59% identity to each other. The former gene is also found to have approximately 75% similarity to the characterized gene in C. kluyveri (Giese) and Simon, Arch. Microbiol 135:51-57 (1983)). It has been reported based on these sequence results that enr is very similar to the dienoyl CoA reductase in E. coli (fadH) (Rohdich et al., J. Biol. Chem. 276:5779-5787 (2001)). The C. thermoaceticum enr gene has also been expressed in a catalytically active form in E. coli (Rohdich et al., J. Biol. Chem. 276:5779-5787 (2001)).
(297) TABLE-US-00046 Gene GenBank name GI# Accession # Organism Enr 169405742 ACA54153.1 Clostridium botulinum A3 str Enr 2765041 CAA71086.1 Clostridium tyrobutyricum Enr 3402834 CAA76083.1 Clostridium kluyveri Enr 83590886 YP_430895.1 Moorella thermoacetica fadH 16130976 NP_417552.1 Escherichia coli
(298) Another candidate enoate reductase is 3-oxoadipate oxidoreductase (maleylacetate reductase), an enzyme catalyzing the reduction of 2-maleylacetate (4-oxohex-2-enedioate) to 3-oxoadipate. The enzyme activity was identified and characterized in Pseudomonas sp. strain B13 (Kaschabek and Reineke, J. Bacteriol. 177:320-325 (1995); and Kaschabek. and Reineke, J. Bacteriol. 175:6075-6081 (1993)), and the coding gene was cloned and sequenced (Kasberg et al., J. Bacteriol. 179:3801-3803 (1997)). Candidate genes for 3-oxoadipate oxidoreductase include cicE gene from Pseudomonas sp. strain B13 (Kasberg et al., J. Bacteriol. 179:3801-3803 (1997)), macA gene from Rhodococcus opacus (Seibert et al., J. Bacteriol. 180:3503-3508 (1998)), and macA gene from Ralstonia eutropha (also known as Cupriavidus necator) (Seibert et al., Microbiology 150:463-472 (2004)).
(299) TABLE-US-00047 Gene GenBank name GI# Accession # Organism clcE 3913241 O30847.1 Pseudomonas sp. strain B13 macA 7387876 O84992.1 Rhodococcus opacus macA 5916089 AAD55886 Cupriavidus necator
(300) Enoyl-CoA reductase enzymes are suitable enzymes for catalyzing the reduction of 2,3-dehydroadipyl-CoA to adipyl-CoA (
(301) TABLE-US-00048 Gene GenBank name GI# Accession # Organism Bcd 15895968 NP_349317.1 Clostridium acetobutylicum etfA 15895966 NP_349315.1 Clostridium acetobutylicum etfB 15895967 NP_349316.1 Clostridium acetobutylicum TER 62287512 Q5EU90.1 Euglena gracilis TDE0597 42526113 NP_971211.1 Treponema denticola
(302) Additional enoyl-CoA reductase enzyme candidates are found in organisms that degrade aromatic compounds. Rhodopseudomonas palustris, a model organism for benzoate degradation, has the enzymatic capability to degrade pimelate via beta-oxidation of pimeloyl-CoA. Adjacent genes in the pim operon, pimC and pimD, bear sequence homology to C. acetobutylicum bcd and are predicted to encode a flavin-containing pimeloyl-CoA dehydrogenase (Harrison and Harwood, Microbiology 151:727-736 (2005)). The genome of nitrogen-fixing soybean symbiont Bradyrhizobium japonicum also contains a pim operon composed of genes with high sequence similarity to pimC and pimD of R. palustris (Harrison and Harwood, Microbiology 151:727-736 (2005)).
(303) TABLE-US-00049 Gene GenBank name GI# Accession # Organism pimC 39650632 CAE29155 Rhodopseudomonas palustris pimD 39650631 CAE29154 Rhodopseudomonas palustris pimC 27356102 BAC53083 Bradyrhizobium japonicum pimD 27356101 BAC53082 Bradyrhizobium japonicum
(304) An additional candidate is 2-methyl-branched chain enoyl-CoA reductase (EC 1.3.1.52), an enzyme catalyzing the reduction of sterically hindered trans-enoyl-CoA substrates. This enzyme participates in branched-chain fatty acid synthesis in the nematode Ascarius suum and is capable of reducing a variety of linear and branched chain substrates including 2-methylbutanoyl-CoA, 2-methylpentanoyl-CoA, octanoyl-CoA and pentanoyl-CoA (Duran et al., J Biol. Chem. 268:22391-22396 (1993)). Two isoforms of the enzyme, encoded by genes acad1 and acad, have been characterized.
(305) TABLE-US-00050 Gene GenBank name GI# Accession # Organism acad1 2407655 AAC48316.1 Ascarius suum Acad 347404 AAA16096.1 Ascarius suum
(306) 1.4.1.a Oxidoreductase (ketone or aldehyde to amino). Oxidoreductases in the EC class 1.4.1 that convert an aldehyde or ketone to its corresponding amine group catalyze several biosynthetic steps in the disclosed pathways. In
(307) Most aminating oxidoreductases catalyze the reversible oxidative deamination of alpha-amino acids with NAD+ or NADP+ as acceptor, and the reactions are typically reversible. Exemplary enzymes include glutamate dehydrogenase (deaminating), encoded by gdhA, leucine dehydrogenase (deaminating), encoded by ldh, and aspartate dehydrogenase (deaminating), encoded by nadX. The gdhA gene product from Escherichia coli (Korber et al., J. Mol. Biol. 234:1270-1273 (1993); and McPherson et al., Nucleic Acids Res. 11:5257-5266 (1983)), gdh from Thermotoga maritime (Kort et al., Extremophiles. 1:52-60 (1997); Lebbink et al., J Mol. Biol. 280:287-296 (1998); and Lebbink et al., J Mol. Biol. 289:357-369 (1999)), and gdhA1 from Halobacterium salinarum (Ingoldsby et al., Gene 349:237-244 (2005)) catalyze the reversible interconversion of glutamate to 2-oxoglutarate and ammonia, while favoring NADP(H), NAD(H), or both, respectively. The ldh gene of Bacillus cereus encodes the LeuDH protein that has a wide of range of substrates including leucine, isoleucine, valine, and 2-aminobutanoate (Ansorge and Kula, Biotechnol Bioeng 68:557-562 (2000); and Stoyan et al., J Biotechnol. 54:77-80 (1997)). The nadX gene from Thermotoga maritime encoding for the aspartate dehydrogenase is involved in the biosynthesis of NAD (Yang et al., J Biol. Chem. 278:8804-8808 (2003)).
(308) TABLE-US-00051 Gene GenBank name GI# Accession # Organism gdhA 118547 P00370 Escherichia coli Gdh 6226595 P96110.4 Thermotoga maritima gdhA1 15789827 NP_279651.1 Halobacterium salinarum Ldh 61222614 P0A393 Bacillus cereus nadX 15644391 NP_229443.1 Thermotoga maritima
(309) Lysine 6-dehydrogenase (deaminating), encoded by lysDH, catalyzes the oxidative deamination of the 6-amino group of L-lysine to form 2-aminoadipate-6-semialdehyde, which in turn non-enzymatically cyclizes to form Δ.sup.1-piperideine-6-carboxylate (Misono and Nagasaki, J. Bacteriol. 150:398-401 (1982)). Exemplary enzymes can be found in Geobacillus stearothermophilus (Heydari et al., Appl Environ. Microbiol 70:937-942 (2004)), Agrobacterium tumefaciens (Hashimoto et al., J Biochem. 106:76-80 (1989); and Misono and Nagasaki, J. Bacteriol. 150:398-401 (1982)), and Achromobacter denitrificans (Ruldeekulthamrong et al., BMB. Rep. 41:790-795 (2008)). Such enzymes are particularly good candidates for converting adipate semialdehyde to 6-aminocaproate given the structural similarity between adipate semialdehyde and 2-aminoadipate-6-semialdehyde.
(310) TABLE-US-00052 Gene GenBank name GI# Accession # Organism lysDH 13429872 BAB39707 Geobacillus stearothermophilus lysDH 15888285 NP_353966 Agrobacterium tumefaciens lysDH 74026644 AAZ94428 Achromobacter denitrificans
(311) 2.3.1.a Acyltransferase (transferring CoA to phospho). Acyltransferases that exchange a CoA moiety for a phosphate are in the EC class 2.3.1. Transformations in this category include the conversions of 6-AAHOP to 6-acetamidohexanoyl-CoA (
(312) TABLE-US-00053 Gene GenBank name GI# Accession # Organism Pta 16130232 NP_416800.1 Escherichia coli Ptb 15896327 NP_349676 Clostridium acetobutylicum Ptb 38425288 AAR19757.1 butyrate-producing bacterium L2-50 Ptb 10046659 CAC07932.1 Bacillus megaterium
(313) 2.3.1.c Acyltransferase (N-acetyltransferase). N-Acetyltransferases transfer an acetyl group to an amine, forming an N-acetyl group. N-Acetylation serves diverse functions in biological systems including transcriptional regulation, nuclear import, chromosome assembly and nucleosome remodeling (Kouzarides, EMBO J 19:1176-1179 (2000)). N-Acetylation of metabolic intermediates of arginine biosynthetic pathways serves both to protect reactive intermediates from spontaneous cyclization and also to sequester pathway intermediates from competing pathways (Caldovic and Tuchman, Biochem. J 372:279-290 (2003)). Acetylation of 6-ACA (
(314) One candidate enzyme for acetylating 6-ACA is lysine N-acetyltransferase (EC 2.3.1.32), an enzyme which selectively transfers the acetyl moiety from acetyl phosphate to the terminal amino group of L-lysine, beta-L-lysine or L-ornithine. Although this enzyme is not known to acetylate 6-ACA, this substrate is structurally similar to the natural substrate. Lysine N-acetyltransferase has been characterized in Bos taurus (Paik. and Kim, Arch. Biochem. Biophys. 108:221-229, 1964) and Methanosarcina mazei (Pfluger et al., Appl Environ. Microbiol 69:6047-6055 (2003)). Methanogenic archaea M. maripaludis, M. acetivorans, M. barkeri and M. jannaschii are also predicted to encode enzymes with this functionality (Pfluger et al., Appl Environ. Microbiol 69:6047-6055 (2003)).
(315) TABLE-US-00054 Gene GenBank name GI# Accession # Organism ablB 21227037 NP_632959.1 Methanosarcina mazei yodP 44921183 CAF30418 Methanococcus maripaludis MA3978 20092772 NP_618847.1 Methanosarcina acetivorans MJ0635 15668816 NP_247619.1 Methanocaldococcus jannaschii Mbar_A0671 73668215 YP_304230.1 Methanosarcina barkeri
(316) Alternately, 6-ACA acetylation can be catalyzed by an enzyme in the GNAT family of N-acetyltransferases. Such enzymes transfer an acetyl group from acetyl-CoA to a primary amine. The enzyme spermidine N-acetyltransferase (S SAT), also known as diamine N-acetyltransferase (EC 2.3.1.57), is capable of acetylating a variety of small molecule substrates. Purified enzymes from Ascaris suum and Onchocerca volvulus exhibit a broad substrate range that includes HMD (Davids et al., Mol. Biochem. Parasitol. 64:341-344 (1994); and Wittich and Walter, Mol. Biochem. Parasitol. 38:13-17 (1990)), but the associated genes have not been identified to date. Other enzymes with this functionality are found in Bacillus subtilis (Forouhar et al., J. Biol. Chem. 280:40328-40336 (2005)) and Homo sapiens (Casero and Pegg, FASEB J 7:653-661 (1993)). A closely related enzyme is thialysine N-acetyltransferase in C. elegans, an enzyme that accepts a range of substrates including lysine, ornithine, thialysine and others (bo-Dalo et al., Biochem. J 384:129-137 (2004)). Amino acid residues involved in substrate binding were identified in the thialysine N-acetyltransferase from Leishmania major (Luersen, K., FEBS Lett. 579:5347-5352 (2005)). An additional candidate is the diaminobutyrate acetyltransferase (EC 2.3.1.178), an enzyme participating in ectoine biosynthesis in Methylomicrobium alcaliphilum (Reshetnikov et al., Arch. Microbiol 184:286-297 (2006)) C. salexigens (formerly Halomonas elongata) (Canovas et al., Syst. Appl Microbiol 21:487-497 (1998)).
(317) TABLE-US-00055 Gene GenBank name GI# Accession # Organism paiA 16080268 NP_391095.1 Bacillus subtilis SSAT1 114322 P21673 Homo sapiens D2023.4 17559148 NP_505978.1 Caenorhabditis elegans LmjF36.2750 68129928 CAJ09234.1 Leishmania major ectA 68366269 AAY96770.1 Methylomicrobium alcaliphilum 20Z ectA 6685422 Q9ZEU8.1 Chromohalobacter salexigens
(318) An additional enzyme candidate for acetylating 6-ACA (
(319) TABLE-US-00056 Gene GenBank name GI# Accession # Organism argJ 16078185 NP_389002.1 Bacillus subtilis ECM40 (ARG7) 6323707 NP_013778.1 Saccharomyces cerevisiae Rv1653 15608791 NP_216169.1 Mycobacterium tuberculosis
(320) 2.3.1.d Acyltransferase (formate C-acyltransferase). The acylation of ketoacids HODH, OHED and 2-OHD to their corresponding CoA derivatives (
(321) TABLE-US-00057 Gene name GI# Accession # Organism pflB 16128870 NP_415423.1 Escherichia coli pflA 16128869 NP_415422.1 Escherichia coli tdcE 48994926 AAT48170.1 Escherichia coli pflD 11499044 NP_070278.1 Archaeglubus fulgidus Pfl 2500058 Q46266.1 Clostridium pasteurianum Act 1072362 CAA63749.1 Clostridium pasteurianum pfl1 159462978 XP_001689719.1 Chlamydomonas reinhardtii pflA1 159485246 XP_001700657.1 Chlamydomonas reinhardtii
(322) 2.6.1.a Aminotransferase. Steps E, H and J of
(323) TABLE-US-00058 Gene name GI# GenBank Accession # Organism gabT 16130576 NP_417148.1 Escherichia coli puuE 16129263 NP_415818.1 Escherichia coli Abat 37202121 NP_766549.2 Mus musculus gabT 70733692 YP_257332.1 Pseudomonas fluorescens Abat 47523600 NP_999428.1 Sus scrofa
(324) Additional enzyme candidates include putrescine aminotransferases or other diamine aminotransferases. Such enzymes are particularly well suited for carrying out the conversion of 6-aminocaproate semialdehyde to HMD. The E. coli putrescine aminotransferase is encoded by the ygjG gene and the purified enzyme also was able to transaminate cadaverine and spermidine (Samsonova et al., BMC. Microbiol 3:2 (2003)). In addition, activity of this enzyme on 1,7-diaminoheptane and with amino acceptors other than 2-oxoglutarate (e.g., pyruvate, 2-oxobutanoate) has been reported (Kim, J Biol. Chem. 239:783-786 (1964); and Samsonova et al., BMC. Microbiol 3:2 (2003)). A putrescine aminotransferase with higher activity with pyruvate as the amino acceptor than alpha-ketoglutarate is the spuC gene of Pseudomonas aeruginosa (Lu et al., J. Bacteriol. 184:3765-3773 (2002)).
(325) TABLE-US-00059 Gene name GI# GenBank Accession # Organism ygjG 145698310 NP_417544 Escherichia coli spuC 9946143 AAG03688 Pseudomonas aeruginosa
(326) Additional candidate enzymes include beta-alanine/alpha-ketoglutarate aminotransferases which produce malonic semialdehyde from beta-alanine (WO08027742). The gene product of SkPYD4 in Saccharomyces kluyveri was shown to preferentially use beta-alanine as the amino group donor (Andersen and Hansen, Gene 124:105-109 (1993)). SkUGA1 encodes a homologue of Saccharomyces cerevisiae GABA aminotransferase, UGA1 (Ramos et al., Eur. J. Biochem. 149:401-404 (1985)), whereas SkPYD4 encodes an enzyme involved in both β-alanine and GABA transamination (Andersen and Hansen, Gene 124:105-109 (1993)). 3-Amino-2-methylpropionate transaminase catalyzes the transformation from methylmalonate semialdehyde to 3-amino-2-methylpropionate. The enzyme has been characterized in Rattus norvegicus and Sus scrofa and is encoded by Abat 1968 (Kakimoto et al., Biochim. Biophys. Acta 156:374-380 (1968); and Tamaki et al., Methods Enzymol. 324:376-389 (2000)).
(327) TABLE-US-00060 GenBank Gene name GI# Accession # Organism SkyPYD4 98626772 ABF58893.1 Saccharomyces kluyveri SkUGA1 98626792 ABF58894.1 Saccharomyces kluyveri UGA1 6321456 NP_011533.1 Saccharomyces cerevisiae Abat 122065191 P50554.3 Rattus norvegicus Abat 120968 P80147.2 Sus scrofa
(328) Steps J and H of
(329) TABLE-US-00061 GenBank Gene name GI# Accession # Organism aspC 16128895 NP_415448.1 Escherichia coli AAT2 1703040 P23542.3 Saccharomyces cerevisiae ASP5 20532373 P46248.2 Arabidopsis thaliana Got2 112987 P00507 Rattus norvegicus avtA 49176374 YP_026231.1 Escherichia coli serC 16128874 NP_415427.1 Escherichia coli
(330) 2.7.2.a Phosphotransferase (carboxy acceptor). Phosphotransferase enzymes in the EC class 2.7.2 transform carboxylic acids to phosphonic acids with concurrent hydrolysis of one ATP. Steps A and E in
(331) TABLE-US-00062 GenBank Gene name GI# Accession # Organism buk1 15896326 NP_349675 Clostridium acetobutylicum buk2 20137415 Q97II1 Clostridium acetobutylicum buk2 6685256 Q9X278.1 Thermotoga maritima lysC 16131850 NP_418448.1 Escherichia coli ackA 16130231 NP_416799.1 Escherichia coli proB 16128228 NP_414777.1 Escherichia coli
(332) Acetylglutamate kinase phosphorylates acetylated glutamate during arginine biosynthesis and is a good candidate for phosphorylating 6-acetamidohexanoate (
(333) TABLE-US-00063 Gene name GI# Accession # Organism argB 145698337 NP_418394.3 Escherichia coli argB 16078186 NP_389003.1 Bacillus subtilis ARG5,6 6320913 NP_010992.1 Saccharomyces cerevisiae
(334) 2.8.3.a Coenzyme-A transferase. Coenzyme-A (CoA) transferases catalyze the reversible transfer of a CoA moiety from one molecule to another. In Step M of
(335) TABLE-US-00064 GenBank Gene name GI# Accession # Organism cat1 729048 P38946.1 Clostridium kluyveri cat2 172046066 P38942.2 Clostridium kluyveri cat3 146349050 EDK35586.1 Clostridium kluyveri TVAG_395550 123975034 XP_001330176 Trichomonas vaginalis G3 Tb11.02.0290 71754875 XP_828352 Trypanosoma brucei
(336) A CoA transferase that can utilize acetyl-CoA as the CoA donor is acetoacetyl-CoA transferase, encoded by the E. coli atoA (alpha subunit) and atoD (beta subunit) genes (Korolev et al., Acta Crystallogr. D. Biol. Crystallogr. 58:2116-2121 (2002); and Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)). This enzyme has a broad substrate range (Sramek and Frerman, Arch. Biochem. Biophys. 171:14-26 (1975)) and has been shown to transfer the CoA moiety to acetate from a variety of branched and linear acyl-CoA substrates, including isobutyrate (Matthies and Schink, Appl Environ. Microbiol 58:1435-1439 (1992)), valerate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)) and butanoate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)). This enzyme is induced at the transcriptional level by acetoacetate, so modification of regulatory control may be necessary for engineering this enzyme into a pathway (Pauli and Overath, Eur. J Biochem. 29:553-562 (1972)). Similar enzymes exist in Corynebacterium glutamicum ATCC 13032 (Duncan et al., Appl. Environ. Microbiol 68:5186-5190 (2002)), Clostridium acetobutylicum (Cary et al., Appl. Environ. Microbiol 56:1576-1583 (1990); and Wiesenborn et al., Appl. Environ. Microbiol 55:323-329 (1989)), and Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol Biochem. 71:58-68 (2007)).
(337) TABLE-US-00065 Gene name GI# GenBank Accession # Organism AtoA 2492994 NP_416726 Escherichia coli K12 AtoD 2492990 NP_416725 Escherichia coli K12 actA 62391407 YP_226809.1 Corynebacterium glutamicum ATCC 13032 cg0592 62389399 YP_224801.1 Corynebacterium glutamicum ATCC 13032 ctfA 15004866 NP_149326.1 Clostridium acetobutylicum ctfB 15004867 NP_149327.1 Clostridium acetobutylicum ctfA 31075384 AAP42564.1 Clostridium saccharoperbutylacetonicum ctfB 31075385 AAP42565.1 Clostridium saccharoperbutylacetonicum
(338) The glutaconyl-CoA-transferase (EC 2.8.3.12) enzyme from anaerobic bacterium Acidaminococcus fermentans reacts with glutaconyl-CoA and 3-butenoyl-CoA (Mack et al., Eur. J. Biochem. 226:41-51 (1994)). The genes encoding this enzyme are gctA and gctB. This enzyme has reduced but detectable activity with other CoA derivatives including glutaryl-CoA, 2-hydroxyglutaryl-CoA, adipyl-CoA and acrylyl-CoA (Buckel et al., Eur. J Biochem. 118:315-321 (1981)). The enzyme has been cloned and expressed in E. coli (Mack et al., Eur. J. Biochem. 226:41-51 (1994)).
(339) TABLE-US-00066 Gene name GI# GenBank Accession # Organism gctA 559392 CAA57199.1 Acidaminococcus fermentans gctB 559393 CAA57200.1 Acidaminococcus fermentans
(340) Yet another CoA transferase is the two-unit succinyl-CoA:3:oxoacid-CoA transferase encoded by pcaI and pcaJ in Pseudomonas putida (Kaschabek et al., J. Bacteriol. 184:207-215 (2002)). Similar enzymes based on homology exist in Acinetobacter sp. ADP1 (Kowalchuk et al., Gene 146:23-30 (1994)). Additional exemplary succinyl-CoA:3:oxoacid-CoA transferases are present in Helicobacter pylori (Corthesy-Theulaz et al., J. Biol. Chem. 272:25659-25667 (1997)) and Bacillus subtilis (Stols et al., Protein Expr. Purif. 53:396-403 (2007)).
(341) TABLE-US-00067 Gene GeneBank name GI# Accession # Organism pcaI 24985644 AAN69545.1 Pseudomonas putida pcaJ 26990657 NP_746082.1 Pseudomonas putida pcaI 50084858 YP_046368.1 Acinetobacter sp. ADP1 pcaJ 141776 AAC37147.1 Acinetobacter sp. ADP1 pcaI 21224997 NP_630776.1 Streptomyces coelicolor pcaJ 21224996 NP_630775.1 Streptomyces coelicolor HPAG1_0676 108563101 YP_627417 Helicobacter pylori HPAG1_0677 108563102 YP_627418 Helicobacter pylori ScoA 16080950 NP_391778 Bacillus subtilis ScoB 16080949 NP_391777 Bacillus subtilis
(342) 3.5.1.a Hydrolase (acting on linear amides). Deacetylation of linear acetamides is catalyzed by an amidohydrolase in the 3.5.1 family of enzymes. Such an enzyme is required for the deacetylation of 6-acetamidohexanamine to HMD (
(343) 2. Acetylpolyamine amidohydrolase (EC 3.5.1.62), is another candidate enzyme that forms the diamines putrescine and cadaverine from their acetylated precursors. The acetylpolyamine deacetylase (AphA) from Mycoplana ramosa has been cloned in E. coli and characterized (Sakurada et al., J. Bacteriol. 178:5781-5786 (1996)) and a crystal structure is available (Fujishiro et al., Biochem. Biophys. Res. Commun. 157:1169-1174 (1988)). This enzyme has also been studied in Micrococcus luteus, but the associated gene has not been identified to date (Suzuki et al., Biochim. Biophys. Acta 882:140-142 (1986)). A protein the histone deacetylase superfamily with high sequence similarity to AphA was identified in the M. luteus genome (evalue=1e-18, 37% identity). The N-acetyl-L-ornithine deacetylase from E. coli is another candidate amidohydrolase (EC 3.5.1.16). The E. coli enzyme, encoded by the argE gene (McGregor et al., J Am. Chem. Soc. 127:14100-14107 (2005); and Meinnel et al., J. Bacteriol. 174:2323-2331 (1992)), removes N-acetyl groups from a variety of substrates including ornithine, lysine, glutamine, and other amino acids (Javid-Majd and Blanchard, Biochemistry 39:1285-1293 (2000)).
(344) TABLE-US-00068 GenBank Gene name GI# Accession # Organism aphA 3023317 Q48935.1 Mycoplana ramose MlutDRAFT_1143 172071524 EDT57566.1 Micrococcus luteus argE 16131795 NP_418392.1 Escherichia coli
(345) 4.1.1.a Carboxy-lyase. Steps D and F in
(346) TABLE-US-00069 Gene name GI# GeneBank Accession # Organism Pdc 118391 P06672.1 Zymomonas mobilus pdc1 30923172 P06169 Saccharomyces cerevisiae Pdc 20385191 Q8L388 Acetobacter pasteurians pdc1 52788279 Q12629 Kluyveromyces lactis
(347) Like PDC, benzoylformate decarboxylase (EC 4.1.1.7) has a broad substrate range and has been the target of enzyme engineering studies. The enzyme from Pseudomonas putida has been extensively studied and crystal structures of this enzyme are available (Hasson et al., Biochemistry 37:9918-9930 (1998); and Polovnikova et al., Biochemistry 42:1820-1830 (2003)). Site-directed mutagenesis of two residues in the active site of the Pseudomonas putida enzyme altered the affinity (Km) of naturally and non-naturally occurring substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)). The properties of this enzyme have been further modified by directed engineering (Lingen et al., Protein Eng 15:585-593 (2002); and Lingen et al., Chembiochem. 4:721-726 (2003)). The enzyme from Pseudomonas aeruginosa, encoded by mdlC, has also been characterized experimentally (Barrowman et al., FEMS Microbiology Letters 34:57-60 (1986)). Additional gene candidates from Pseudomonas stutzeri, Pseudomonas fluorescens and other organisms can be inferred by sequence homology or identified using a growth selection system developed in Pseudomonas putida (Henning et al., Appl. Environ. Microbiol. 72:7510-7517 (2006)).
(348) TABLE-US-00070 Gene Gen Bank name GI# Accession # Organism mdIC 3915757 P20906.2 Pseudomonas putida mdIC 81539678 Q9HUR2.1 Pseudomonas aeruginosa dpgB 126202187 ABN80423.1 Pseudomonas stutzeri ilvB-1 70730840 YP_260581.1 Pseudomonas fluorescens
(349) A third enzyme capable of decarboxylating 2-oxoacids is alpha-ketoglutarate decarboxylase (KGD). The substrate range of this class of enzymes has not been studied to date. The KDC from Mycobacterium tuberculosis (Tian et al., Proc Natl Acad Sci USA 102:10670-10675 (2005)) has been cloned and functionally expressed in other internal projects at Genomatica. However, it is not an ideal candidate for strain engineering because it is large (.sup.˜130 kD) and GC-rich. KDC enzyme activity has been detected in several species of rhizobia including Bradyrhizobium japonicum and Mesorhizobium loti (Green et al., J. Bacteriol. 182:2838-2844 (2000)). Although the KDC-encoding gene(s) have not been isolated in these organisms, the genome sequences are available and several genes in each genome are annotated as putative KDCs. A KDC from Euglena gracilis has also been characterized but the gene associated with this activity has not been identified to date (Shigeoka and Nakano, Arch. Biochem. Biophys. 288:22-28 (1991)). The first twenty amino acids starting from the N-terminus were sequenced MTYKAPVKDVKFLLDKVFKV (SEQ ID NO: 1) (Shigeoka and Nakano, Arch. Biochem. Biophys. 288:22-28 (1991)). The gene can be identified by testing candidate genes containing this N-terminal sequence for KDC activity.
(350) TABLE-US-00071 GenBank Gene name GI# Accession # Organism Kgd 160395583 O50463.4 Mycobacterium tuberculosis Kgd 27375563 NP_767092.1 Bradyrhizobium japonicum Kgd 13473636 NP_105204.1 Mesorhizobium loti
(351) A fourth candidate enzyme for catalyzing this step is branched chain alpha-ketoacid decarboxylase (BCKA). This class of enzyme has been shown to act on a variety of compounds varying in chain length from 3 to 6 carbons (Oku and Kaneda, J Biol Chem. 263:18386-18396 (1988); and Smit et al., Appl Environ Microbiol. 71:303-311 (2005)). The enzyme in Lactococcus lactis has been characterized on a variety of branched and linear substrates including 2-oxobutanoate, 2-oxohexanoate, 2-oxopentanoate, 3-methyl-2-oxobutanoate, 4-methyl-2-oxobutanoate and isocaproate (Smit et al., Appl Environ Microbiol. 71:303-311 (2005)). The enzyme has been structurally characterized (Berg et al., Science. 318:1782-1786 (2007)). Sequence alignments between the Lactococcus lactis enzyme and the pyruvate decarboxylase of Zymomonas mobilus indicate that the catalytic and substrate recognition residues are nearly identical (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)), so this enzyme would be a promising candidate for directed engineering Decarboxylation of alpha-ketoglutarate by a BCKA was detected in Bacillus subtilis; however, this activity was low (5%) relative to activity on other branched-chain substrates (Oku and Kaneda, J Biol Chem. 263:18386-18396 (1988)) and the gene encoding this enzyme has not been identified to date. Additional BCKA gene candidates can be identified by homology to the Lactococcus lactis protein sequence. Many of the high-scoring BLASTp hits to this enzyme are annotated as indolepyruvate decarboxylases (EC 4.1.1.74). Indolepyruvate decarboxylase (IPDA) is an enzyme that catalyzes the decarboxylation of indolepyruvate to indoleacetaldehyde in plants and plant bacteria.
(352) TABLE-US-00072 Gene Gen Bank name Gl Accession # Organism kdcA 44921617 AAS49166.1 Lactococcus lactis
(353) Recombinant branched chain alpha-keto acid decarboxylase enzymes derived from the E1 subunits of the mitochondrial branched-chain keto acid dehydrogenase complex from Homo sapiens and Bos taurus have been cloned and functionally expressed in E. coli (Davie et al., J. Biol. Chem. 267:16601-16606 (1992); Wynn et al., J. Biol. Chem. 267:1881-1887 (1992); and Wynn et al., J. Biol. Chem. 267:12400-12403 (1992)). In these studies, the authors found that co-expression of chaperonins GroEL and GroES enhanced the specific activity of the decarboxylase by 500-fold (Wynn et al., J. Biol. Chem. 267:12400-12403 (1992)). These enzymes are composed of two alpha and two beta subunits.
(354) TABLE-US-00073 Gene GenBank name Gl# Accession # Organism BCKDHB 34101272 NP_898871.1 Homo sapiens BCKDHA 11386135 NP_000700.1 Homo sapiens BCKDHB 115502434 P21839 Bos taurus BCKDHA 129030 P11178 Bos taurus
(355) The decarboxylation of 2-AHD to 6-aminocaproate (
(356) TABLE-US-00074 Gene name Gl# Accession # Organism panD 67470411 P0A790 Escherichia coli K12 panD 18203593 Q9X4NO Corynebacterium glutamicum panD 54041701 P65660.1 Mycobacterium tuberculosis
(357) 4.1.2.a Aldehyde-lyase. HOHD aldolase, also known as HHED aldolase, catalyzes the conversion of 4-hydroxy-2-oxo-heptane-1,7-dioate (HOHD) into pyruvate and succinic semialdehyde (
(358) TABLE-US-00075 Gene GenBank name Gl# Accession # Organism hpcH 633197 CAA87759.1 Escherichia coli C hpal 38112625 AAR11360.1 Escherichia coli W
(359) 4.2.1.a Hydro-lyase. The enzyme OHED hydratase participates in 4-hydroxyphenylacetic acid degradation, where it converts 2-oxo-hept-4-ene-1,7-dioate (OHED) to 2-oxo-4-hydroxy-hepta-1,7-dioate (HODH) using magnesium as a cofactor (Burks et al., J. Am. Chem. Soc. 120 (1998)) (
(360) TABLE-US-00076 Gene Gene Bank name Gl# Accession # Organism hpcG 556840 CAA57202.1 Escherichia coli C hpaH 757830 CAA86044.1 Escherichia coli W hpaH 150958100 ABR80130.1 Klebsiella pneumoniae Sari_01896 160865156 ABX21779.1 Salmonella enterica
(361) Dehydration of 3-hydroxyadipyl-CoA to 2,3-dehydroadipyl-CoA (
(362) TABLE-US-00077 Gene Gene Bank name Gl# Accession # Organism Crt 15895969 NP_349318.1 Clostridium acetobutylicum crt1 153953091 YP_001393856.1 Clostridium kluyveri
(363) Enoyl-CoA hydratases (EC 4.2.1.17) also catalyze the dehydration of 3-hydroxyacyl-CoA substrates (Agnihotri and Liu., J. Bacteriol. 188:8551-8559 (2003); Conrad et al., J. Bacteriol. 118:103-111 (1974); and Roberts et al., Arch. Microbiol 117:99-108 (1978)). The enoyl-CoA hydratase of Pseudomonas putida, encoded by ech, catalyzes the conversion of 3-hydroxybutyryl-CoA to crotonoyl-CoA (Roberts et al., Arch. Microbiol 117:99-108 (1978)). Additional enoyl-CoA hydratase candidates are phaA and phaB, of P. putida, and paaA and paaB from P. fluorescens (Olivera et al., Proc. Nall. Acad. Sci U.S.A 95:6419-6424 (1998)). The gene product of pimF in Rhodopseudomonas palustris is predicted to encode an enoyl-CoA hydratase that participates in pimeloyl-CoA degradation (Harrison and Harwood, Microbiology 151:727-736 (2005)). Lastly, a number of Escherichia coli genes have been shown to demonstrate enoyl-CoA hydratase functionality including maoC (Park and Lee, J. Bacteriol. 185:5391-5397 (2003)), paaF (Ismail et al., J. Biochem. 270:3047-3054 (2003); Park and Lee, Appl. Biochem. Biotechnol 113-116:335-346 (2004); and Park and Yup, Biotechnol Bioeng 86:681-686 (2004)) and paaG (Ismail et al., J. Biochem. 270:3047-3054 (2003); Park and Lee, Appl. Biochem. Biotechnol 113-116:335-346 (2004); and Park and Yup, Biotechnol Bioeng 86:681-686 (2004)).
(364) TABLE-US-00078 Gene GenBank name Gl# Accession # Organism Ech 26990073 NP_745498.1 Pseudomonas putida paaA 26990002 NP_745427.1 Pseudomonas putida paaB 26990001 NP_745426.1 Pseudomonas putida phaA 106636093 ABF82233.1 Pseudomonas fluorescens phaB 106636094 ABF82234.1 Pseudomonas fluorescens pimF 39650635 CAE29158 Rhodopseudomonas palustris maoC 16129348 NP_415905.1 Escherichia coli paaF 16129354 NP_415911.1 Escherichia coli paaG 16129355 NP_415912.1 Escherichia coli
(365) Alternatively, the E. coli gene products of fadA and fadB encode a multienzyme complex involved in fatty acid oxidation that exhibits enoyl-CoA hydratase activity (Nakahigashi and Inokuchi, Nucleic Acids Res. 18:4937 (1990); Yang, J. Bacteriol. 173:7405-7406 (1991); and Yang et al., Biochemistry 30:6788-6795 (1991)). Knocking out a negative regulator encoded by fadR can be utilized to activate the fadB gene product (Sato et al., J Biosci. Bioeng 103:38-44 (2007)). The fadI and fadJ genes encode similar functions and are naturally expressed under anaerobic conditions (Campbell et al., Mol. Microbiol 47:793-805 (2003)).
(366) TABLE-US-00079 Gene GenBank name Gl# Accession # Organism fadA 49176430 YP_026272.1 Escherichia coli fadB 16131692 NP_418288.1 Escherichia coli fadI 16130275 NP_416844.1 Escherichia coli fadJ 16130274 NP_416843.1 Escherichia coli fadR 16129150 NP_415705.1 Escherichia coli
(367) 6.2.1.a Acid-thiol ligase (also called CoA synthetase). Steps I and M of
(368) TABLE-US-00080 Gene GenBank name Gl# Accession # Organism AF1211 11498810 NP_070039.1 Archaeoglobus fulgidus DSM 4304 AF1983 11499565 NP_070807.1 Archaeoglobus fulgidus DSM 4304 Scs 55377722 YP_135572.1 Haloarcula marismortui ATCC 43049 PAE3250 18313937 NP_560604.1 Pyrobaculum aerophilum str. IM2 sucC 16128703 NP_415256.1 Escherichia coli sucD 1786949 AA073823.1 Escherichia coli
(369) Another candidate enzyme for this step is 6-carboxyhexanoate-CoA ligase, also known as pimeloyl-CoA ligase (EC 6.2.1.14), which naturally activates pimelate to pimeloyl-CoA during biotin biosynthesis in gram-positive bacteria. The enzyme from Pseudomonas mendocina, cloned into E. coli, was shown to accept the alternate substrates hexanedioate and nonanedioate (Binieda et al., Biochem. J 340 (Pt 3):793-801 (1999)). Other candidates are found in Bacillus subtilis (Bower et al., J. Bacteriol. 178:4122-4130 (1996)) and Lysinibacillus sphaericus (formerly Bacillus sphaericus) (Ploux et al., Biochem. J 287 (Pt 3):685-690 (1992)).
(370) TABLE-US-00081 Gene Gen Bank name Gl# Accession # Organism pauA 15596214 NP_249708.1 Pseudomonas mendocina bioW 50812281 NP_390902.2 Bacillus subtilis bioW 115012 P22822.1 Lysinibacillus sphaericus
(371) Additional CoA-ligases include the rat dicarboxylate-CoA ligase for which the sequence is yet uncharacterized (Vamecq et al., Biochem. J 230:683-693 (1985)), either of the two characterized phenylacetate-CoA ligases from P. chrysogenum (Lamas-Maceiras et al., Biochem. J 395:147-155 (2006); and Wang et al., Biochem. Biophys. Res. Commun. 360:453-458 (2007)) and the phenylacetate-CoA ligase from Pseudomonas putida (Martinez-Blanco et al., J. Biol. Chem. 265:7084-7090 (1990)). Additional candidate enzymes are acetoacetyl-CoA synthetases from Mus musculus (Hasegawa et al., Biochim. Biophys. Acta 1779:414-419 (2008)) and Homo sapiens (Ohgami et al., Biochem. Pharmacol. 65:989-994 (2003)) which naturally catalyze the ATP-dependant conversion of acetoacetate into acetoacetyl-CoA.
(372) TABLE-US-00082 Gene GenBank name Gl# Accession # Organism Phl 77019264 CAJ15517.1 Penicillium chlysogenum phlB 152002983 ABS19624.1 Penicillium chlysogenum paaF 22711873 AA024333.2 Pseudomonas putida AACS 21313520 NP_084486.1 Mus musculus AACS 31982927 NP_076417.2 Homo sapiens
The invention will now be described in more detail on the basis of the following nonlimiting examples and with reference to the accompanying figures.
EXAMPLES
Example 1
(373) This experiment illustrates HMD/CO.sub.2 pH equilibrium under changing conditions. In this example, a 10% w/w aqueous HMD solution was prepared (70% HMD purchased from Sigma Aldrich). The HMD solution was heated to 30° C. and initial pH recorded. Next, CO.sub.2 (approximately 98% pure) was bubbled into the solution for 60 min while monitoring pH. The CO.sub.2 sparge was stopped. Air was sparged into solution for 30 min while monitoring pH. The air sparge was kept on and the solution was heated to 80° C. for 40 min. The pH was measured by sampling 3 mL to a falcon tube and cooling to 30° C. The solution was heated to 88° C. for 20 min. The solution was cooled to 30° C. and a final pH recorded. The measured pH values are plotted in Graphs 1-1, 1-2 and 1-3, below. It was noted that the temperature probe on pH meter was reading 44.4° C. at 300 min. and cooled back down to 30° C. by the end of the 60 min. This was most likely caused by the exothermic acid/base reaction. This temperature increase can also account for the nonlinearity in
(374) TABLE-US-00083 TABLE 1-1 Conditions and pH response Condition pH (temp. change in ° C.) Comments Initial conditions 12.25 (30) pH probe calibrated at 22° C. Sparge CO.sub.2 for 60 min. 7.96 (30) at 30° C. House air sparge for 8.23 (30) 10 bubbles/sec. sparge rate 30 min. at 30° C. Heated to 80° C. for 10.49 (30) pH measure in a sample 30 min. w/ sparge cooled to 30° C. Heated to 88° C. 10.76 (30) pH measured in a sample for 15 min. w/ sparge 11.02 (22.5) and after bulk cooled down. Readings agreed +/−0.01 Sat open to air over 10.78 (20.6) Some decrease in pH night overnight.
(375) Table 1-2 shows simulated molar ratio of CO.sub.2:HMD based on pH. It can be observed that 1.8 equivalents of CO.sub.2 go into solution very quickly. The rate of absorption starts to slow down significantly when 2 equivalents are reached.
(376) TABLE-US-00084 TABLE 1-2 HMD:CO.sub.2 in solution for selected points based on pH simulation. Simulated CO.sub.2:HMD (molar pH ratio in solution) 10.76 0.523 9.77 1.314 9.00 1.820 8.23 1.994 7.96 2.038 12.433 Control for = 0.86 M, pKw = 13.840
(377) The results illustrate that air and heat can restore the pH all the way back up to 11.02. Extraction should be possible at this pH. Aeration during fermentation may be able to strip off some of the dissolved CO.sub.2.
Example 2
(378) This example reports the results of a simulated fermentation process. Briefly, CO.sub.2 (approximately 98% pure) and HMD (70% HMD from Sigma Aldrich) were fed into an MM9 solution that had the following composition: 0.68% (6.8 g/L) Disodium phosphate Na.sub.2HPO.sub.4 0.3% (3 g/L) Monopotassium phosphate KH.sub.2PO.sub.4 0.15% (1.5 g/L) Ammonium chloride NH.sub.4Cl 0.1% (1 g/L) Ammonium sulfate (NH.sub.4).sub.2SO.sub.4 0.05% (0.5 g/L) Sodium chloride NaCl
The experimental conditions for this example are listed in Table 2-1.
(379) TABLE-US-00085 TABLE 2-1 Summary of Experimental conditions Input Number Unit Feed rate HMD 0.6 mL/min Feed [HMD] 1.16 mol/L Initial reactor volume 0.6 L Total time of exp. 24 hours Final solution volume 1.464 L Final [HMD] 0.685 mol/L Molar ratio CO.sub.2 3.2 to 1 HMD Temp. of inlet CO.sub.2 21 ° C. CO.sub.2 feed rate 54.8 mL/min Air to CO.sub.2 ratio 4 to 1 CO.sub.2 Air feed rate 219 mL/min Total gas sparge rate 274 mL/min
(380) The measured pH of the solution and concentration of HMD added over time are set out in
(381) This feed rate used in this example corresponds to an average rate of 3.31 g/L/hr (a reasonable production rate in an industrial fermentation process). The final titer and feed concentration of HMD was confirmed by LCMS. The HMD and CO.sub.2 reach a dynamic equilibrium around pH 8.5. As more HMD is added, more CO.sub.2 is absorbed. The simulated HMD:CO.sub.2 ratio is 1.95 for pH=8.53. This ratio indicates that the major species in solution is the HMD.sup.2+(HCO.sub.3.sup.−).sub.2 salt (hexamethylenediamine bis-bicarbonate).
(382) At pH 8.5 (dashed, black line) the major species in solution are the diprotonated HMD and bicarbonate. A minority presence of HMD-carbamate may be present as well.
Example 3
(383) This example demonstrates the generation of HMD, as the free base, from the protonated and/or carbonate/carbamate compounds formed by contacting an aqueous solution of HMD with CO.sub.2 gas, where the aqueous solution was MM9 medium.
(384) A 250 mL four-neck flask was fitted with a condenser, temperature probe, and air sparge needle. The system was open to air through the top of the condenser. The feed was an aqueous HMD solution in MM9 where pH was adjusted to 8.68 with CO2. The feed solution was analyzed for HMD concentration by LCMS and the pH was measured. The solution was sparged with air and refluxed at 85° C. for three hours. The resulting solution's pH was taken and the concentration of HMD measured by LCMS.
(385) TABLE-US-00086 TABLE 3-1 Summary of regeneration results Stream pH [HMD] mol/L CO.sub.2:HMD ratio* Feed 8.68 @ 834.0 1.91 21.7° C. Product 11.26 @ 954.7 0.277 23.5° C. *Based on calculation from pH.
(386) The positive pH change from 8.68 to 11.26 shown in Table 3-1 indicates that CO.sub.2 was stripped out of solution. The concentration increased slightly as some water escaped through the condenser and the solution was concentrated. The fraction of HMD in free base form at pH 11.26 is 57%.
Example 4
(387) The example describes the solvent extraction of HMD from the CO.sub.2 stripped solution prepared in Example 3 above as well as extracting HMD from an aqueous solution without pH adjustment as a control for solvent performance. The following protocol was used in this example:
(388) The pH of the aqueous feed was measured. In a 50 mL falcon tube, 20 g of solvent was mixed with 20 g of feed. This combination was further aggressively mixed in the falcon tube for 5 minutes and vortexed for 1 minute. The tube with the mixed solvent and feed was allowed to settle until phase separation was complete. The volume of the bottom, aqueous layer was recorded. A sample of the top layer was carefully pipetted out the top layer. A sample the bottom layer was also obtained and the pH of the bottom layer measured. The recovery, distribution coefficients and selectivity based on mass balance were calculated.
(389) The extraction data for three solvents extracting HMD from water are set out in Table 4-1.
(390) TABLE-US-00087 TABLE 4-1 Results of solvent screening. HMD in deionized (DI) water. [HMD] = 835 mM % pH pH % water in % Solvent used* Solubility before after extract Selectivity recovery 1-hexanol 0.59 12.70 12.31 11.68 10.76 66.4 Isopentanol 2.8 12.70 12.16 9.08 19.07 71.4 Cyclohexanol 3.6 12.70 12.36 16.65 8.13 70.9 Hexane 9.4 × 10.sup.−4 12.55 12.54 <0.001 >1,000 9.1 *All solvents were received from Sigma Aldrich at >97% purity
(391) Based on the screening reported in Table 4-1, 1-hexanol was initially used for extracting the product of the HMD regeneration solution prepared in Example 3 above because it has the lower water solubility than isopentanol and cyclohexanol. Alkanes, specifically hexane, were screened and subsequently tested due to extremely low water solubility. Hexane extracted little if any water and provided reasonable recovery of the available free base.
(392) Table 4-2 illustrates that with 1:1 solvent to feed ratio, HMD may be extracted in 25.8% overall recovery or 4% in the case of hexane. Based on the pH before extraction, only 60% of free base HMD is available. Thus, approximately 43% of the available free base HMD was extracted by the solvent 1-hexanol and about 7% by hexane. Hexane extracted little if any water and provided reasonable recovery of the available free base.
(393) TABLE-US-00088 TABLE 4-2 Results of extracting stripped solution from “regeneration experiment.” [HMD] = 955 mM % pH pH % water in % Solvent used Solubility before after extract Selectivity recovery 1-hexanol 0.59 11.44 11.02 9.02 2.70 25.8 Hexane 9.4 × 10−4 11.48 11.42 <0.001 >1,000 4.0
Example 5
(394) Comparative Example
(395) When a modeled HMD fermentation was controlled to a pH 7 with H2SO4, assuming 88% of glucose is converted to HMD on a mass basis, and a final HMD titer of 116 g/L, 0.843 g H2SO4 per g HMD is needed to maintain the pH at 7. Due to the amount of sulfuric acid used, carbon dioxide is not readily absorbed. This resulted in a final DIC/TDCA value of less than 0.5%. The fermentation model takes into account, among other things, cellular growth and respiration, byproduct formation, and media composition needed.
Example 5A
(396) When an HMD fermentation was modeled at a pH of 8.5, assuming 88% of glucose is converted to HMD on a mass basis, and a final HMD titer of 116 g/L, no sulfuric acid is needed to maintain the pH of 8.5 (based on experimental results). Carbon dioxide is readily absorbed by the HMD. During the seed fermentation, the DIC/TDCA rose from <1% to approximately 54%. During the product fermentation, the value rose from approximately 54% to approximately 96%. The fermentation model takes into account, among other things, cellular growth and respiration, byproduct formation, and media composition needed.
Example 5B
(397) When a modeled HMD fermentation is controlled to a pH of 7 with only CO2, assuming 88% of glucose is converted to HMD on a mass basis, and a final HMD titer of 116 g/L, It is shown that 2.4 moles of CO2 would need to be absorbed per mole of HMD. It has been experimentally shown in other examples that a pH of 7 can be reached with CO2 as the only acid used in pH control. During the seed fermentation model, the DIC/TDCA rose from <1% to approximately 82%. During the product fermentation model, the value rose from approximately 82% to approximately 97%. The fermentation model takes into account, among other things, cellular growth and respiration, byproduct formation, and media composition needed.
Example 6
(398) Solvents such as alkanes were evaluated as suitable solvents for HMD free base recovery from aqueous solutions. See
(399) In this example the extraction column had 10 theoretical stages. In this model the HMD comprised about 96.6% free base (solvent extractable form), thus the plotted values slightly underestimate the percent recovery of the recoverable form of HMD. These in silico modeling results demonstrate that alkanes can be effective solvents for HMD recovery from aqueous solutions at a range of solvent to HMD solution ratios.
(400) The efficiency of solvent extraction increases with decrease in DIC concentration as shown below, supporting the importance of CO2 removal. DIC decrease results in higher pH and higher concentration of recoverable free base form.
Example 7
(401) ASPEN Plus was used to model the use of either a water evaporator (a multi-effect evaporator) or a steam stripping column alone to achieve water and CO2 removal prior to HMD recovery. Conditions were as above; the components included in the ASPEN model were water, HMD, DIC species and hexane; an electrolyte NRTL model was used. While either step can be removed, and despite increasing electricity and steam usage in the evaporator when more water is evaporated, a savings in utility costs is realized with use of the evaporator because it is more efficient at removing water than the stripping column. The figure below (
(402) The figure below (
Example 8
(403) Preparation of an HMDA Producing Microbial Organism Having Carbonic Anhydrase
(404) Escherichia coli is used as a target organism to engineer to produce HMDA having nucleic acids encoding the enzymes utilized in the HMDA pathway and carbonic anhydrase.
(405) The gene encoding a Desulfovibrio vulgais (GenBank accession ACL09337.1 GI:218758438, SEQ ID) is codon-optimized for expression in E. coli and is cloned into an expression vector under the control of a constitutive promoter. This vector also contains an origin of replication and an antibiotic resistance gene. Also cloned into an expression vector or integrated into the host, E. coli in this example, are genes encoding enzymes for production of a diamine, e.g, HMD.
(406) The resulting plasmids are transformed into E. coli, for example MG1655 or ATCC 8739, by chemical transformation or electroporation. For chemical transformation, cells are grown to mid-log growth phase, as determined by the optical density at 600 nm (0.5-0.8). The cells are harvested, washed and finally treated with CaCl2). To chemically transform these E. coli cells, purified plasmid DNA is allowed to mix with the cell suspension in a microcentrifuge tube on ice. A heat shock is applied to the mixture and followed by a 30-60 minute recovery incubation in rich culture medium. For electroporation, E. coli cells grown to mid-log growth phase are washed with water several times and finally resuspended into 10% glycerol solution. To electroporate DNA into these cells, a mixture of cells and DNA is pipetted into a disposable plastic cuvette containing electrodes. A short electric pulse is then applied to the cells to form small holes in the membrane where DNA could enter. The cell suspension is then incubated with rich liquid medium followed by plating on solid agar plates. Detailed protocol is described in Molecular Cloning: A Laboratory Manual Third Edition, Sambrook and Russell, 2001, Cold Spring Harbor Laboratory Press, 3rd Edition.
(407) The resulting genetically engineered E. coli is cultured in glucose-containing medium following procedures well known in the art (see, for example, Sambrook et al., supra, 2001). The expression of the carbonic anhydrase and HMDA genes are corroborated using methods well known in the art for determining polypeptide expression or enzymatic activity, including for example, Northern blots, PCR amplification of mRNA, immunoblotting, and the like. Enzymatic activities of the expressed enzymes are confirmed using assays specific for the individual activities. The ability of the engineered E. coli strain to produce carbonic anhydrase can be confirmed using phenolphtheleine pH indicator to monitor pH change as carbonic anhydrase converts carbonate to CO.sub.2 and the resulting pH of the solution containing the DA increases. The color change of phenolphtheleine can be monitored by absorbance at 550 nm. An assay is conducted by adding 300 mM-1000 mM DA to a cell lysate or the extracellular media with 300 mM-400 mM KHCO.sub.3 and 400 μM-1100 μM phenolphthalein (Alvizo, et. al. 2014 PNAS 111(46): 16436-16441). Another assay for carbonic anhydrase activity is a colorimetric assay using 4-nitrophenylacetate as a substrate; 3 mM 4-nitrophenylacetate (Verpoorte et. al. 1967 J. Biol. Chem. 242: 4221-4229. Carbonic anhydrase can also be monitored by production of 002. HMD can be confirmed by HPLC.