Acetate derived compounds from geranylorcinol. synthesis process for obtaining said compounds and use of said compounds as antifungal against Botrytis cinerea

Abstract

The present invention discloses a synthesis process for obtaining linear derivative compounds from geranylorcinoles, said linear derivative compounds from geraniylorcinol, the acetylated derivatives compounds therefrom, the method for encapsulating the compounds in a polymer matrix and the use of said encapsulated compounds as antifungal against Botrytis cinerea.

Claims

1. An encapsulated compound, wherein said encapsulated compound is a polymeric micelle containing a compound selected from the group consisting of a compound of Formula 1, a compound of Formula 2, a compound of Formula 3, a compound of Formula 4, a compound of Formula 5, or a compound of Formula 6: ##STR00003## ##STR00004## ##STR00005##

2. A method of preparing the encapsulated compound of claim 1, the method comprising: (i) dissolving the compound in a solvent; (ii) adding an aqueous polymer solution; (iii) stirring in a vortex and applying ultrasound until a homogeneous emulsion is formed; and (iv) removing the solvent by evaporation.

3. A method of treating a fungal infection in a subject, the method comprising: administering the encapsulated compound according to claim 1 as an antifungal agent, to the subject in need thereof having the fungal infection in an amount effective for treating the fungal infection.

4. The method according to claim 3, wherein said fungal infection is caused by Botrytis cinerea.

5. The encapsulated compound of claim 1, wherein the polymeric micelle consists of a polymer selected from polycaprolactone or Pluronic F-127.

6. The encapsulated compound of claim 1, wherein the compound is selected from the group consisting of a compound of Formula 1, a compound of Formula 2, a compound of Formula 4, or a compound of Formula 6.

7. The encapsulated compound of claim 1, wherein the encapsulated compound is formed by emulsion encapsulation.

Description

DETAILED DESCRIPTION OF THE INVENTION

(1) The present invention discloses a synthesis process for obtaining linear derivative compounds from geranylorcinol, said linear derivative compounds from geranylorcinol, the acetylated derivative compounds thereof, the method for encapsulating the compounds in a polymer matrix and the use of said encapsulated compounds as antifungal against Botrytis cinerea.

(2) The synthesis process comprises condensation of geraniol with orcinol (Friedel-Crafts allylation) catalysed by a Lewis acid. The process comprises using the Lewis acid BF.sub.3.Et.sub.2O catalyzed direct alkylation technique and the use of a AgNO.sub.3 secondary catalyst employed for the first time in this type of reactions.

(3) The synthesis process allowed the di-coupled derivative compounds represented by the formulas 1 to 4 to be obtained, from which the compounds of formula 2 and 4 correspond to compounds claimed in the present invention. The structures of the linear geranylorcinoles, obtained by the coupling method of the present invention, using the secondary AgNO.sub.3 catalyst are shown in formulas 1 to 4.

(4) ##STR00001##

(5) The compounds derived from the formulas 1 and 3, were previously synthesized by other authors (Manners, G.; Jurd, L.; and Stevens, K.; Tetrahedron, 1972, 28, 2949-2959; Eisohly, H. N.; Turner, C. E.; Clark, A. M.; and Eisohly, M. A.; J. Pharmaceutical Sciences, 1982, 71, 1319-1323) with yields of between 2-18% and also previously described by our research group, with yields of 12.6% and 27.6% for the derived compound 3 and 1 (Taborga, L., Vergara, A., Fernndez, MJ, Osorio, M, Carvajal, M, Madrid, A, Marilaf, F, Carrasco, H, and Espinoza, Journal of the Chilean Chemical Society, 2013, 58, 1790-1796).

(6) Additionally, the acetylated derivative compounds of the four compounds represented in formulas 1 to 4, which have been represented by formulas 5 to 8, respectively, were prepared. Formulas 5 to 8, correspond to the following structures:

(7) ##STR00002##

(8) The acetylated derivative compounds 5 to 8 correspond to structures claimed in the present invention. These derivative compounds were obtained from the respective geranylorcinol derivative compounds of formulas 1 to 4, through acetylation reaction under standard conditions; that is, by acetylation with acetic anhydride, 4-N,N-dimethylaminopyridine (DMAP) as catalyst and dichloromethane (CH.sub.2Cl.sub.2) as the solvent.

(9) Experimental Procedure

(10) Synthesis Process of the Geranylorcinol Derived Compounds of the Formulas 1, 2, 3 and 4

(11) 2 mL of acetonitrile was added to a solution of orcinol (1.22 g, 9.8 mmol) and geraniol (1.51 g, 9.8 g), and a solution of acetonitrile saturated in AgNO.sub.3 (400 mg in 2 mL) was added thereafter, and then stirred at room temperature under a nitrogen atmosphere. BF.sub.3Et.sub.2O (0.46 g, 3.2 mmol) was directly and slowly (dropwise) injected to this mixture. It was allowed to stir for 48 hours. The term of the reaction was monitored by thin layer chromatography (TLC). To complete the reaction, crushed ice (about 30 g) and abundant salt (about 50 g NaCl) were added, then vacuum filtered and the organic phase was extracted with AcOEt (320 mL), which phase was then washed with a solution of NaHCO.sub.3 (15 mL, 5%) and water (215 mL), then dried over anhydrous Na.sub.2SO.sub.4, this solution was then filtered and evaporated to dryness. The reaction was performed in quadruplicate and the sum of crude reaction product was purified by column chromatography (CC) using silica gel (silica gel 60 for column chromatography, 0.040-0.063 mm) as the stationary phase and a mixture of acetate as the mobile phase (0:20.fwdarw.6:14:starting with a first addition with a total volume of 20 mL of hexane and 0 mL of ethyl acetate, the second addition of the same total volume, but now with 18.8 mL of hexane and 0.2 mL of ethyl acetate, and so on by increasing the amount of ethyl acetate in 0.2 mL and decreasing the amount of hexane in 0.2 mL in each fraction, until the last addition of 6 mL of hexane and 14 mL of ethyl acetate is reached, always maintaining a total and constant volume of 20 mL). A total of 108 fractions were collected in 10 mL flasks. Then, in order to identify the fractions containing the products, a qualitative analysis was performed by Thin Layer Chromatography (TLC) where fractions from 18-28 contained compound 2. They were combined and labelled as F-I 1,554.8 mg (9.9% yield) of a dark yellow viscous oil (formula 2 compound). Fractions from 38-49 were combined and contained compound 3, labelled F-II 1281.8 mg (12.5% yield) of a yellow viscous oil (formula 3 compound). Fractions from 67-73 were combined and contained the compound 4, labelled F-III 955.3 mg (6.1% yield) of a dark yellow viscous oil (formula 4 compound). Fractions from 74-76 were combined and contained the mixture of compounds 1 and 4, labelled F-IV 415.4 mg of a dark yellow viscous oil (mixture of compounds of formula 1 and 4, in a 0.125:1 ratio respectively). Fractions from 77-87 were combined and contained compound 1, labelled F-V 2816.2 mg (27.6% yield) of a yellow viscous oil (formula 1 compound). From fractions 88-108, the starting material (orcinol) which did not react (78.6 mg) was recovered.

(12) Formula 1 Compound Spectroscopic Characterization:

(13) RMN of .sup.1H: 6.26 (s, 1H, H-6); 6.22 (s, 1H, H-2); 5.60 (bs. 2H, OH); 5.13 (t, J=6.8 Hz, 1H, H-2); 5.05 (t, J=6.7 Hz, 1H, H-6); 3.28 (d, J=6.7 Hz, 2H, H-1); 2.21 (s, 3H, H-7); 2.09-2.06 (m, 2H, H-5); 2.04-2.00 (m, 2H, H-4); 1.78 (s, 3H, CH3-C3); 1.67 (s, 3H, H-8); 1.58 (s, 3H, CH3-C7); RMN of .sup.13C: 155.2 (C-3); 154.1 (C-1); 138.6 (C-5); 137.2 (C-3); 131.8 (C-7); 123.9 (C-6); 122.2 (C-2); 118.1 (C-4); 109.7 (C-6); 101.0 (C-2); 39.6 (C-4); 26.4 (C-5); 25.6 (C-8); 25.0 (C-1); 20.0 (C-7); 17.6 (CH3-C7); 16.1 (CH3-C3); IR (cm.sup.1): 3,393, 2,975, 2,925, 2,859, 1,602; EM (m/z, %): M.sup.+ 260 (21.7), 137 (100), 191 (26.9), 177 (13.9), 123 (10.5).

(14) Formula 2 Compound Spectroscopic Characterization:

(15) RMN of .sup.1H: 6.30 (s, 1H, H-6); 5.50 (s, 1H, OH); 5.44 (bs. 1H, OH); 5.32 (t, J=6.9 Hz, 1H, H-2); 5.19 (t, J=6.6 Hz, 1H, H-2); 5.11 (t, J=6.6 Hz, 2H, H-6 y H-6); 3.46 (d, J=6.9 Hz, 2H, H-1); 3.35 (d, J=6.6 Hz, 2H, H-1); 2.25 (s, 3H, CH.sub.3Ar); 2.16-2.15 (m, 4H, H-5 y H-5); 2.12-2.09 (m, 4H, H-4 y H-4); 1.86 (s, 3H, CH.sub.3C3); 1.84 (s, 3H, CH.sub.3C3); 1.73 (s, 6H, CH.sub.3C7 y CH.sub.3C7); 1.64 (s, 6H, H-8 y H-8); RMN of .sup.13C: 153.4 (C-3); 152.5 (C-1); 138.3 (C-3); 137.0 (C-3); 135.1 (C-5); 131.8 (.sup.+C-7); 131.6 (.sup.+C-7); 123.9 (.sup.++C-6); 123.8 (.sup.++C-6); 122.5 (C-2); 122.0 (C-2); 118.1 (C-4); 111.5 (C-2); 109.7 (C-6); 39.6 (C-4); 39.5 (C-4); 26.4 (.sup.+++C-5); 26.3 (.sup.+++C-5); 25.6 (C-8 y C-8); 25.4 (C-1); 22.5 (C-1); 19.7 (CH.sub.3Ar); 17.6 (C-10 y C-10); 16.1 (C-9); 16.0 (C-9); IR (cm.sup.1): 3,454, 2,967, 2,917, 2,855, 1,747, 1,621, 1,592, 1,448, 1,377.

(16) Formula 3 Compound Spectroscopic Characterization:

(17) RMN of .sup.1H: 6.26 (s, 2H, H-6 y H-4); 5.66 (bs. 2H, OH); 5.29 (t, J=6.6 Hz, 1H, H-2); 5.08 (t, J=6.1 Hz, 1H, H-6); 3.42 (d, J=7.0 Hz, 2H, H-1); 2.20 (s, 3H, CH.sub.3Ar); 2.13-2.10 (m, 2H, H-5); 2.09-2.07 (m, 2H, H-4); 1.83 (s, 3H, CH.sub.3C3); 1.70 (s, 3H, H-8); 1.61 (s, 3H, CH.sub.3C7); RMN of .sup.13C: 154.8 (C-1 y C-3); 138.6 (C-3); 137.3 (C-5); 131.9 (C-7); 123.7 (C-6); 121.8 (C-2); 110.6 (C-2); 109.0 (C-4 y C-6); 39.6 (C-4); 26.3 (C-5); 25.6 (C-8); 22.1 (C-1); 21.0 (CH.sub.3Ar); 17.6 (CH.sub.3C7); 16.0 (CH.sub.3C3); IR (cm.sup.1): 3,455, 2,966, 2,922, 2,852, 1,772.

(18) Formula 4 Compound Spectroscopic Characterization:

(19) RMN of 1H: 6.25 (s, 1H, H-2); 5.12 (t, J=6.4 Hz, 2H, H-2); 5.05 (t, J=6.6 Hz, 2H, H-6); 3.33 (d, J=6.6 Hz, 4H, H-1); 2.21 (s, 3H, CH.sub.3Ar); 2.09-2.06 (m, 4H, H-5); 2.03-2.00 (m, 4H, H-4); 1.78 (s, 6H, CH.sub.3C3); 1.66 (s. 6H, H-8); 1.58 (s, 6H, CH.sub.3C7); RMN of .sup.13C: 152.9 (C-1 y C-3); 136.7 (C-3); 136.4 (C-5); 131.7 (C-7); 124.0 (C-6); 122.6 (C-2); 118.5 (C-4 yC-6); 101.4 (C-2); 29.6 (C-4); 26.5 (C-5); 25.7 (C-8); 25.6 (C-1); 17.6 (CH.sub.3C7); 16.2 (CH.sub.3C3); 15.8 (CH.sub.3Ar); IR (cm.sup.1): 3,404, 2,967, 2,922, 2,855, 1,649, 1,599, 1,445, 1,376.

(20) Synthesis Process of the Geranylorcinol Acetylated Derived Compounds of the Formulas 5, 6, 7 and 8.

(21) From each compound one portion (362 mg of the compound of formula 1; 170 mg of the formula 2 compound; 74 mg of the formula 3 compound; and 245 mg of the formula 4 compound) were taken which were separately placed in four reaction balloons with 50 mL of CH.sub.2Cl.sub.2, and then 1.0 mL of acetic anhydride and 2 crystals of 4-N,N-dimethylaminopyridine (DMAP) were added to each balloon. Then each mixture was allowed to stir for about one hour. After completion of the reaction the solution in each balloon was evaporated to dryness and re-suspended in 50 mL of ethyl acetate, which was washed with 30 mL of a 5% sodium bicarbonate solution and water (220 mL), then dried over anhydrous sodium sulphate (20 g), filtered and evaporated to dryness. Finally, the four compounds represented by formulas 5 to 8 were obtained with the following yields: Compound 5: 444.6 mg (92.8% yield) of a dark yellow viscous oil. Compound 6: 185 mg (90.5% yield) of a dark yellow viscous oil. Compound 7: 93.3 mg (96.4% yield) of a dark yellow viscous oil. Compound 8: 267.5 mg (89.8% yield) of a dark yellow viscous oil.

(22) Formula 5 Compound Spectroscopic Characterization:

(23) RMN of .sup.1H: 6.80 (s, 1H, H-6); 6.70 (s, 1H, H-2); 5.04 (t, J=6.3 Hz, 1H, H-6); 4.97 (t, J=6.4 Hz, 1H, H-2); 3.21 (d, J=6.4 Hz, 2H, H-1); 2.28 (s, 6H, COCH.sub.32); 2.26 (s, 3H, CH.sub.3Ar); 2.06-2.02 (m, 2H, H-5); 1.99-1.95 (m, 2H, H-4); 1.73 (s, 3H, CH.sub.3C3); 1.65 (s, 3H, H-8); 1.58 (s, 3H, CH.sub.3C7); RMN of .sup.13C: 169.3 (COCH.sub.3); 169.2 (COCH.sub.3); 149.0 (C-3); 148.3 (C-1); 139.2 (C-5); 135.7 (C-3); 131.4 (C-7); 129.7 (C-4); 124.1 (C-6); 121.2 (C-2); 120.8 (C-6); 113.5 (C-2); 39.5 (C-4); 26.5 (C-5); 25.8 (C-8); 25.6 (C-1); 21.1 (COCH.sub.3); 20.9 (COCH.sub.3); 19.7 (CH.sub.3Ar); 17.6 (CH.sub.3C7); 16.2 (CH.sub.3C3). IR (cm.sup.1): 2,968, 2,927, 2,851, 1,774, 1,752, 1,367; EM (m/z, %): 274 (10.8), 259 (4.1), 192 (12.3), 191(100), 176 (8.3).

(24) Formula 6 Compound Spectroscopic Characterization:

(25) RMN of .sup.1H: 6.79 (s, 1H, H-6); 5.08-5.04 (m, 3H, H-6, H-6 y H-2); 5.00 (t, J=7.0 Hz, 1H, H-2); 3.17 (bs. 4H, H-1 y H-1); 2.28 (s, 3H, COCH.sub.3); 2.26 (s, 6H, COCH.sub.3 y CH.sub.3Ar); 2.08-2.04 (m, 4H, H-5 y H-5); 2.00-1.96 (m, 4H, H-4 y H-4); 1.73 (s, 3H, *CH.sub.3C3); 1.71 (s, 3H, *CH.sub.3C3); 1.67 (s, 6H, H-8 y H-8); 1.59 (s, 6H, CH.sub.3C7 y CH.sub.3C7); RMN of .sup.13C: 169.3 (COCH.sub.3); 169.0 (COCH.sub.3); 148.1 (C-3); 147.2 (C-1); 136.2 (C-5); 135.4 (.sup.+C-3); 135.2 (.sup.+C-3); 131.3 (C-7 y C-7); 130.2 (C-4); 124.1 (C-6 y C-6); 123.9 (C-2); 121.8 (C-6); 121.5 (.sup.++C-2); 121.4 (.sup.++C-2); 39.5 (C-4 y C-4); 26.5 (C-5 y C-5); 26.4 (C-1); 25.6 (C-8 y C-8); 24.2 (C-1); 20.8 (COCH.sub.3); 20.5 (COCH.sub.3); 19.4 (CH.sub.3Ar); 17.6 (CH.sub.3C7 y CH.sub.3C7); 16.1 (.sup.+++CH.sub.3C3); 16.0 (.sup.+++CH.sub.3C3).

(26) Formula 7 Compound Spectroscopic Characterization:

(27) RMN of .sup.1H: 6.77 (s, 2H, H-4 y H-6); 5.06 (t, J=6.3 Hz, 1H, H-6); 5.02 (t, J=6.9 Hz, 1H, H-2); 3.14 (d, J=6.9 Hz, 2H, H-1); 2.32 (s, 3H, CH.sub.3Ar); 2.27 (s, 6H, COCH.sub.3); 2.07-2.01 (m, 2H, H-5); 1.97-1.93 (m, 2H, H-4); 1.71 (s, 3H, CH.sub.3C3); 1.66 (s, 3H, H-8); 1.58 (s, 3H, CH.sub.3C7); RMN of .sup.13C: 169.1 (COCH.sub.32); 149.4 (C-1 y C-3); 137.0 (C-5); 135.5 (C3); 131.3 (C-7); 124.0 (C-6); 123.3 (C-2); 121.2 (C-2); 120.8 (C-4-C-6); 39.5 (C-4); 26.5 (C-5); 25.6 (C-8); 23.6 (C-1); 20.9 (CH.sub.3Ar); 20.8 (COCH.sub.32); 17.6 (CH.sub.3C7); 16.1 (CH.sub.3C3).

(28) Formula 8 Compound Spectroscopic Characterization:

(29) RMN of .sup.1H: 6.70 (s, 1H, H-2); 5.06 (t, J=6.2 Hz, 2H, H-6); 5.01 (t, J=5.6 Hz, 2H, H-2); 3.26 (d, J=6.2 Hz, 4H, H-1); 2.28 (s, 6H, COCH.sub.3); 2.22 (s, 3H, CH.sub.3Ar); 2.08-2.05 (m, 4H, H-5); 2.01-1.98 (m, 4H, H-4); 1.75 (s, 6H, CH.sub.3C3); 1.67 (s, 6H, H-8); 1.60 (s, 6H, CH.sub.3C7); RMN of .sup.13C: 169.2 (COCH.sub.32); 146.8 (C-1 y C-3); 137.9 (C-5); 135.3 (C-3); 131.2 (C-7); 130.0 (C-4 y C-6); 124.0 (C-6); 121.6 (C-2); 113.9 (C-2); 39.4 (C-4); 26.4 (C-5); 26.2 (C-1); 25.5 (C-8); 20.7 (COCH.sub.32); 17.5 (CH.sub.3C7); 16.0 (CH.sub.3C3); 15.4 (CH.sub.3Ar).

(30) Evaluation of the Antifungal Activity of the Compounds Synthesized Against Botrytis Cinerea in Free State and Encapsulated System.

(31) Preparation of the Stock Solution of Each Synthesized Compound without Encapsulating.

(32) 20-40 milligrams of each of compounds 1 to 8 were dissolved in 500 microliters of Ethanol to have a stock solution of each compound according to its molecular weight. The stock solution was diluted in 5 mL of water so as to obtain a new stock solution of 5,000 ppm; from which corresponding aliquots were taken to achieve a final concentration on each plate with 50, 150 and 250 ppm potato dextrose agar (PDA) culture medium.

(33) Preparation of Stock Solution of Each Compound Synthesized in a Polymer Solution Pluronic F-127.

(34) The method used for the preparation of polymeric micelles, based on Polycaprolactone (MW: 3.409 g/mol) or Pluronic F-127 (MW: 12,500 g/mol) corresponds to the direct dissolution method consisting of the addition of the polymer to water with stirring and at room temperature, until the polymer dissolves to give a clear aqueous solution. The amount of polymer and water is chosen so as to have a solution of a concentration of 110.sup.3 M of polymer chains.

(35) For the preparation of a solution of 50 mL of Pluronic F-127, 0.63 g is used and for the case of polycaprolactone 0.17 g is used.

(36) The physical encapsulation of the compounds in the polymeric micelles (110.sup.3 M) is carried out by the emulsion method. The compound is dissolved in a volatile solvent (dichloromethane) and 10 mL of the polymer solution added in 100 L aliquots to complete 400 L reaching a concentration of 0.101 M of compound. By stirring in a vortex and applying ultrasound a very homogeneous milky emulsion is formed. The organic solvent is then removed by evaporation by heating the emulsion at 50 C. in a system open to air. Upon removal of the solvent, a clear solution remains containing the polymer forming micelles and the non-polar compounds which have been encapsulated in the hydrophobic micro-domain provided by the core of these polymeric micelles.

(37) Subsequently, the solution is diluted by adding polymer solution corresponding to a 110.sup.3 M concentration until the compound of interest reaches a stock concentration in the range of 625-1,250 ppm in the micelle to be applied to the plate. This dilution should be done by adding polymer solution so as to maintain the same concentration of micelles and vary only the concentration of active ingredient.

(38) Anti-Phytopathogenic Activity Test Against B. cinerea.

(39) In order to determine the inhibition of mycelial growth of B. cinerea, both for the free state and encapsulated compounds, the radial growth method in potato dextrose agar (PDA) culture medium was used. The compounds of interest in both free and encapsulated form were added to the PDA at different concentrations of 50, 150 and 250 ppm at a temperature of 50 C. Once the PDA had been solidified, a 4-mm diameter disc was seeded with mycelium of the pathogen in the center of the petri dish. The experiment consists of a negative control which is a petri dish where PDA culture medium and 1% Ethanol were added, in the case of Negative Control for encapsulated compounds it consists in adding only the micelle (polycaprolactone or Pluronic F-167) free in PDA. In addition, a positive control containing commercially available fungicide Captan in PDA culture medium is included at the same concentrations tested in both its free form and also a positive control of Captan in its encapsulated form. Each treatment consisted of three replicates (n=3 replicates/compound/concentration).

(40) After 48 (free state) and 72 (encapsulated system) hours of incubation at 23 C. under a photoperiod of 16 light hours/8 darkness hours, the mycelial growth inhibition percentage (MGIP) was calculated by assessing the measurement of the diameter of the fungus growth around the disc to calculate the mycelial growth inhibition area. In this way, the mycelial growth inhibition percentage that was compared to the corresponding controls after 48 hours of incubation was determined.

(41) Results

(42) Table 1 shows that all the compounds derived from formulas 1 to 8 in their free state affect the mycelial growth of the pathogenic fungus B. cinerea in vitro between 51-97% at concentrations ranging from 150 to 250 ppm, as compared to the negative control after 48 hours of incubation. Compound 6 was found to be the most active by inhibiting mycelial growth by 97% at 250 ppm. The inhibition result is directly dependent on the concentration in which it is applied against the pathogen.

(43) The inhibitory capacity is increased when the compounds are encapsulated in polymeric micelles of different origin. In the case of compounds derived from formulas 2, 4, 6 and 8 the biological activity in vitro increases when encapsulated in micelles formed by Pluronic F-127 to obtain a percentage of mycelial inhibition at 50 ppm between 81-99% as compared to the negative control after 72 hours. However, when these compounds are encapsulated in polycaprolactone micelles, only the compound of formula 4 improves its biological activity by 93% at 50 ppm, whereas the compounds derived from formulas 2, 6 to 8 do not produce any inhibiting effect on the mycelium of the pathogen as they are encapsulated in caprolactone micelles.

(44) In the case of the mono-coupled compound of formula 1 (previously described in the literature) its activity at lower concentrations is also slightly improved when encapsulated in polycaprolactone as compared to the control, unlike the mono-coupled compound of formula 3 (previously described in the literature) which was inactive against the mycelium of the fungus when encapsulated in polycaprolactone.

(45) The results indicate that the biological activity of mycelial inhibition is improved when the compounds are encapsulated in polymeric micelles; depending on its structure, it may be inferred that for the di-coupled compounds derived from formulas 2, 4, 6 and 8 it is more effective to use a polymeric Pluronic F-127 micelle, since its activity becomes equal to the activity of the positive control.

(46) In the case of mono-coupled compounds derived from formulas 1, 3, 5 and 7 their effectiveness will depend on their structural composition when encapsulated in a micelle of polycaprolactone diblock.

(47) TABLE-US-00001 TABLE 1 Mycelial inhibition percentage of the compounds of formula 1-8 both in free system and in encapsulated form in Polycaprolactone and Pluronic F-127 at different concentrations in vitro. Mycelial inhibition percentage of Botrityscinerea in vitro (%) Compounds encapsulated Compounds encapsulated Free compound in Polycaprolactone (Diblock) in Pluronic F-127 (Triblock) 50 150 250 50 150 250 50 150 250 Compound ppm ppm ppm ppm ppm ppm ppm ppm ppm Formula 1 0 25 75 37 61 73 Formula 2 25 61 93 16 36 37 85 91 96 Formula 3 37 65 82 9 32 42 Formula 4 12 56 83 93 94 97 82 92 96 Formula 5 24 55 78 16 39 80 Formula 6 5 69 97 10 31 50 93 99 99 Formula 7 8 51 82 32 35 59 Formula 8 11 54 85 0 36 36 93 99 99 C (negative 0 0 0 0 0 0 0 0 0 control) C+ (positive 87 94 94 93 99 99 98 99 99 control) (): Not tested.