Animal feed stock using microbial enhancement

Abstract

In one aspect, a method for feeding animals includes selecting a plurality of strains of sporulated living microbes and form a microbial solution; and applying the microbial solution to a carrier. In another aspect during use, the method includes feeding animals with the microbial infused carrier to deliver microbes to a gastrointestinal tract alive; and germinating the microbes in the digestive tract and stimulating local intestinal immunity with the microbes.

Claims

1. A method for feeding animals, comprising: selecting a plurality of strains of sporulated living Bacillus species to form a microbial solution comprising: iteratively and selectively breeding generations of the Bacillus species for strain selection having predetermined microbial gene profiles and forming said microbial solution in a highly concentrated form, wherein multiple Bacillus species are separately cultivated and followed with cross cultivation in a specific sequence; selecting as a carrier for a predetermined animal: for grazing animal, one of hull, wheat, and dendritic salt, for poultry, dendritic salt or calcium carbonate, for aquatic animal, dendritic salt, for swine, dendritic salt or calcium carbonate, applying the microbial solution to the carrier to provide a microbial infused carrier; and performing feed blending between 0.01 to 1 gram said microbial infused carrier per kilogram of feed as a pre-mixed feed or coating for feeding said predetermined animal.

2. The method of claim 1, comprising feeding animals with the blended microbial infused carrier to deliver microbes to a gastrointestinal tract alive.

3. The method of claim 2, comprising germinating the microbes in a digestive tract and stimulating local intestinal immunity with the microbes.

4. The method of claim 1, comprising selecting the microbe from Bacillus (B.) acidiceler, B. acidicola, B. acidiproducens, B. acidocaldarius, B. acidoterrestrisr, B. aeolius, B. aerius, B. aerophilus, B. agaradhaerens, B. agri, B. aidingensis, B. akibai, B. alcalophilus, B. algicola, B. alginolyticus, B. alkalidiazotrophicus, B. alkalinitrilicus, B. alkalisediminis, B. alkalitelluris, B. altitudinis, B. alveayuensis, B. alvei, B. amyloliquefaciens, B. a. subsp. amyloliquefaciens, B. a. subsp. plantarum, B. amylolyticus, B. andreesenii, B. aneurinilyticus, B. anthracis, B. aquimaris, B. arenosi, B. arseniciselenatis, B. arsenicus, B. aurantiacus, B. arvi, B. aryabhattai, B. asahii, B. atrophaeus, B. axarquiensis, B. azotofixans, B. azotoformans, B. badius, B. barbaricus, B. bataviensis, B. beijingensis, B. benzoevorans, B. beringensis, B. berkeleyi, B. beveridgei, B. bogoriensis, B. boroniphilus, B. borstelensis, B. brevis Migula, B. butanolivorans, B. canaveralius, B. carboniphilus, B. cecembensis, B. cellulosilyticus, B. centrosporus, B. cereus, B. chagannorensis, B. chitinolyticus, B. chondroitinus, B. choshinensis, B. chungangensis, B. cibi, B. circulans, B. clarkii, B. clausii, B. coagulans, B. coahuilensis, B. cohnii, B. composti, B. curdlanolyticus, B. cycloheptanicus, B. cytotoxicus, B. daliensis, B. decisifrondis, B. decolorationis, B. deserti, B. dipsosauri, B. drentensis, B. edaphicus, B. ehimensis, B. eiseniae, B. enclensis, B. endophyticus, B. endoradicis, B. farraginis, B. fastidiosus, B. fengqiuensis, B. firmus, B. flexus, B. foraminis, B. fordii, B. formosus, B. fortis, B. fumarioli, B. funiculus, B. fusiformis, B. galactophilus, B. galactosidilyticus, B. galliciensis, B. gelatini, B. gibsonii, B. ginsengi, B. ginsengihumi, B. ginsengisoli, B. globisporus, B. g. subsp. globisporus, B. g. subsp. marinus, B. glucanolyticus, B. gordonae, B. gottheilii, B. graminis, B. halmapalus, B. haloalkaliphilus, B. halochares, B. halodenitrificans, B. halodurans, B. halophilus, B. halosaccharovorans, B. hemicellulosilyticus, B. hemicentroti, B. herbersteinensis, B. horikoshii, B. horneckiae, B. horti, B. huizhouensis, B. humi, B. hwajinpoensis, B. idriensis, B. indicus, B. infantis, B. infernus, B. insolitus, B. invictae, B. iranensis, B. isabeliae, B. isronensis, B. jeotgali, B. kaustophilus, B. kobensis, B. kochii, B. kokeshiiformis, B. koreensis, B. korlensis, B. kribbensis, B. krulwichiae, B. laevolacticus, B. larvae, B. laterosporus, B. lautus, B. lehensis, B. lentimorbus, B. lentus, B. licheniformis, B. ligniniphilus, B. litoralis, B. locisalis, B. luciferensis, B. luteolus, B. luteus, B. macauensis, B. macerans, B. macquariensis, B. macyae, B. malacitensis, B. mannanilyticus, B. marisflavi, B. marismortui, B. marmarensis, B. massiliensis, B. megaterium, B. mesonae, B. methanolicus, B. methylotrophicus, B. migulanus, B. mojavensis, B. mucilaginosus, B. muralis, B. murimartini, B. mycoides, B. naganoensis, B. nanhaiensis, B. nanhaiisediminis, B. nealsonii, B. neidei, B. neizhouensis, B. niabensis, B. niacini, B. novalis, B. oceanisediminis, B. odysseyi, B. okhensis, B. okuhidensis, B. oleronius, B. oryzaecorticis, B. oshimensis, B. pabuli, B. pakistanensis, B. pallidus, B. pallidus, B. panacisoli, B. panaciterrae, B. pantothenticus, B. parabrevis, B. paraflexus, B. pasteurii, B. patagoniensis, B. peoriae, B. persepolensis, B. persicus, B. pervagus, B. plakortidis, B. pocheonensis, B. polygoni, B. polymyxa, B. popilliae, B. pseudalcalophilus, B. pseudofirmus, B. pseudomycoides, B. psychrodurans, B. psychrophilus, B. psychrosaccharolyticus, B. psychrotolerans, B. pulvifaciens, B. pumilus, B. purgationiresistens, B. pycnus, B. qingdaonensis, B. qingshengii, B. reuszeri, B. rhizosphaerae, B. rigui, B. ruris, B. safensis, B. salarius, B. salexigens, B. saliphilus, B. schlegelii, B. sediminis, B. selenatarsenatis, B. selenitireducens, B. seohaeanensis, B. shacheensis, B. shackletonii, B. siamensis, B. silvestris, B. simplex, B. siralis, B. smithii, B. soli, B. solimangrovi, B. solisalsi, B. songklensis, B. sonorensis, B. sphaericus, B. sporothermodurans, B. stearothermophilus, B. stratosphericus, B. subterraneus, B. subtilis, B. s. subsp. inaquosorum, B. s. subsp. spizizenii, B. s. subsp. subtilis, B. taeanensis, B. tequilensis, B. thermantarcticus, B. thermoaerophilus, B. thermoamylovorans, B. thermocatenulatus, B. thermocloacae, B. thermocopriae, B. thermodenitrificans, B. thermoglucosidasius, B. thermolactis, B. thermoleovorans, B. thermophilus, B. thermoruber, B. thermosphaericus, B. thiaminolyticus, B. thioparans, B. thuringiensis, B. tianshenii, B. trypoxylicola, B. tusciae, B. validus, B. vallismortis, B. vedderi, B. velezensis, B. vietnamensis, B. vireti, B. vulcani, B. wakoensis, B. weihenstephanensis, B. xiamenensis, B. xiaoxiensis, and B. zhanjiangensis.

5. The method of claim 1, comprising germinating in an upper digestive tract and acting on predetermined sections of an intestine for nutrient absorption.

6. The method of claim 1, wherein the hull comprises corn or almond hull.

7. A method for feeding animals, comprising: selecting a plurality of strains of sporulated living Bacillus species to form a microbial solution comprising: iteratively and selectively breeding generations of the Bacillus species for strain selection having predetermined microbial gene profiles and forming said microbial solution in a highly concentrated form, wherein multiple Bacillus species are separately cultivated and followed with cross cultivation in a specific sequence; selecting as a carrier for a predetermined animal: for grazing animal, one of hull, wheat, and dendritic salt, for poultry, dendritic salt or calcium carbonate, for aquatic animal, dendritic salt, for swine, dendritic salt or calcium carbonate, applying the microbial solution to the carrier to provide a microbial infused carrier; and performing feed blending between 0.01 to 5 gram of said microbial infused carrier per kilogram of feed as a pre-mixed feed or coating for feeding said predetermined animal.

8. A method for feeding animals, comprising: selecting a plurality of strains of sporulated living Bacillus species to form a microbial solution comprising: iteratively and selectively breeding generations of the Bacillus species for strain selection having predetermined microbial gene profiles and forming said microbial solution in a highly concentrated form, wherein multiple Bacillus species are separately cultivated and followed with cross cultivation in a specific sequence; selecting as a carrier for a predetermined animal: for grazing animal, one of hull, wheat, and dendritic salt, for poultry, dendritic salt or calcium carbonate, for aquatic animal, dendritic salt, for swine, dendritic salt or calcium carbonate, applying the microbial solution to the carrier to provide a microbial infused carrier; and mixing between 0.01 to 5 gram of said microbial infused carrier per liter of water as a drink for said predetermined animal.

Description

BRIEF DESCRIPTION OF THE FIGURES

(1) FIG. 1 shows an exemplary process to infuse hulls or shells with microbials for feeding animals.

(2) FIG. 2A shows an exemplary digestive tract with nutrients, non-beneficial bacteria, lactobacilli and probiotics.

(3) FIG. 2B shows exemplary probiotics actions.

(4) FIG. 3 shows an exemplary process to form PROBIOFEED.

DETAILED DESCRIPTION OF THE INVENTION

(5) FIG. 1 shows an exemplary process to infuse hulls or shells with microbials for feeding animals. The process includes: Select a plurality of strains of sporulated living microbes and form a microbial solution (12); Infuse a carrier such as hulls or shells with the microbial solution (14); Feed animals with the microbial infused hulls or shells (16) Microbes reaches gastrointestinal tract alive (18) Germinate in upper digestive tract (20) Stimulate local intestinal immunity with the microbes (22)

(6) Different carriers can be used. Beside hulls, the feed can use wheat or corn powder for carrier. Dendritic salt and grind up limestone or calcium carbonate can be used as well. The purpose of using salt as a carrier is to help the animal restore electrolytes in the body. This problem is often caused by extreme heat in the summer, mineral deficiency in winter and after severe diarrhea. The salt will not only help retain water, electrolytes in the body, but also increases appetite. Wheat, hull, corn carriers provide more nutrients in the diet but should be used when the animal is healthy during a normal growing cycle. For example, grazing animals, the feed includes hull, wheat, and dendritic salt for the carrier, depending on the season. For poultry, the feed includes dendritic salt or calcium carbonate. For aquatic fish and other creatures, dendritic salt is used. For swine, the feed can include dendritic salt or calcium carbonate.

(7) In one embodiment called PROBIOFEED, the solution contains several strains of bacillus spp to ensure a broad spectrum product that can work in wide variety of environmental applications. The Bacillus spp germinates in upper digestive tract and display their activity in those sections of intestine which are relevant for nutrient absorption. Bacillus is selected as a sporulated living microorganism with the ability to form spores. They reach the gastrointestinal tract alive and stimulates local intestinal immunity.

(8) Various cell walls that protect the nucleus from external stresses enable the Bacillus products to withstand massive stress during feed production and storage caused by 1) High temperature, 2) Pressure, 3) Shear forces, and 4) Oxidation impacts. In some embodiments, selected cell walls are used as a protective structure/mechanism for producing the Bacillus included products.

(9) FIG. 2A shows an exemplary digestive tract with nutrients, non-beneficial bacteria, lactobacilli and probiotics. As detailed in FIG. 2B, probiotics actions include: blocking of adhesion sites, production of inhibitory substances, competition for nutrients, and influencing the immunity system. The benefits include improved digestibility of nutrients and detoxification of toxic molecules and improved vitamin synthesis (B and K). This provides an environmental friendly animal husbandry.

(10) The probiotics provide antagonistic action against non-desirable microorganisms (barrier effect) and protects the intestinal mucous membrane against invading microorganisms. They contribute to maturation and stimulation of the host's immune system; improve growth and survivability of the animal; and reduce feed cost. The probiotics also improve feed conversion (FCR), decrease by 1%-5% and improves daily weight gain (DWG), increase by 3%-5%. Yet other benefits include one or more of the following: Increased production and survivability Reduced risk of digestive problems Improved nutrients absorption Uniform growth and better homogeneity of the groups Reduced fattening period Reduced feed expenditure Reduced medication costs Reduced slurry nutrient content (lower nitrogen excretion for example)

(11) Next, exemplary results on probiotics on the performance of animals are shown as follows:

(12) Influence of Various Probiotics on the Performance of Animals

(13) TABLE-US-00001 Production branch DWG (% of control) FCR (% of control) Piglet production +4.8 1.5 (8.1 to +24.3) (+3.1 to 9.3) Calf production +5.4 2.5 (5.3 to +21.7) (+3.6 to 7.9) Growing/fattening pigs +3.7 5.1 (0.3 to +6.7) (1.4 to 7.1) Growing/fattening cattle +3.4 2.7 (4.3 to +7.2) (+7.6 to 4.7)

(14) Influence of Probiotics on Protein Digestibility and Crude Protein Deposition in Piglets

(15) TABLE-US-00002 Nitrogen Nitrogen deposition digestibility (%) (g W.sup.0.75 per day).sup.1 Control Probiotic** Control Probiotic** Authors 81.05.sup.a 82.86.sup.b 1.24.sup.a 1.34.sup.b SE Scheuermann, 1993 78.70.sup.c 83.20.sup.c 1.76 1.81 Tossenberger et al., 1995 .sup.1Relative to metabolic body weight **dosage 1 10.sup.9 CFU per kg of piglet feed .sup.a, b, c, dsignificant differences

(16) To feed the animals, in various embodiments: For feed blending as a pre-mixed or coating: about 0.5 gram per kilogram of feed For per feed mixing (can be mixed to one meal per day): about 2 grams per kilogram of feed For drinking dosage: about 2 grams per liter of water.

(17) The microbes used for animal nutrition have a very good safety record. Even in cases of overdoses of more than thousand times recommended levels in feed, there're no signs of dysbiosis in the gastrointestinal tract. Probiotics do not constitute any health hazard for animal. Since they are not transferred from intestine into the body of animal, probiotics do not affect any metabolic processes, nor do they have any negative impact on the animal.

(18) FIG. 3 shows an exemplary process to form PROBIOFEED. First, the master seed culture is identified. Next, the process compares the master seed and starter culture with the original isolates. One or more cell cultures are produced in fermenters, and the result can be concentrated by centrifugation, among others. The concentrated cell cultures can be dried using lyophilisation, stabilization, and standardization techniques. Quality control is then done and the result can be compared with the master seed culture. Additional protection measures can be applied, such as coating the dried cell cultures. The result can be mixed with a carrier if necessary, and then quality control can be done. The resulting PROBIOFEED composition can be packaged for shipping. PRO BIOFEED Final Packaging can include Bags/Buckets/Drums, among others.

(19) Various exemplary microbial compositions are detailed next.

Example 1

(20) Microbes: Bacillus pumilus 4.0510{circumflex over ()}8 CFU/g Bacillus subtilis 6.3010{circumflex over ()}8 CFU/g Bacillus amyloliquefaciens 5.8510{circumflex over ()}8 CFU/g Bacillus lichniformis 1.8010{circumflex over ()}8 CFU/g

(21) Amino Acids: Amino Acids

(22) Dendritic Salt: Sodium Chloride

Example 2

(23) Microbes: Bacillus licheniformis 2.2810{circumflex over ()}9 CFU/g Bacillus subtilis 2.2810{circumflex over ()}9 CFU/g

(24) Dendritic Salt: Sodium Chloride

Example 3

(25) Microbes: Bacillus licheniformis 4.76210{circumflex over ()}9 CFU/g

(26) Amino Acids: Amino Acids

(27) Ground Lime Stone: Calcium Carbonate

Example 4

(28) Microbes: Bacillus licheniformis 2.2810{circumflex over ()}9 CFU/g Bacillus subtilis 2.2810{circumflex over ()}9 CFU/g

(29) Almond Hull: Ground Almond Hull

Example 5

(30) Microbes: Bacillus licheniformis 4.76210{circumflex over ()}9 CFU/g

(31) Amino Acids: Amino Acids

(32) Dendritic Salt: Sodium Chloride

Example 6

(33) Microbes: Bacillus Subtilis 4.76210{circumflex over ()}9 CFU/g

(34) Amino Acids: Amino Acids

(35) Dendritic Salt: Sodium Chloride

(36) The above description is for the purpose of illustrating and not limiting the present invention, and teaching the person of ordinary skill in the art how to practice the invention. It is not intended to detail all those obvious modifications and variations of it which will become apparent to the skilled worker upon reading the description. It is intended, however, that all such obvious modifications and variations be included within the scope of the present invention as defined in the appended claims. The claims are meant to cover the claimed components and steps in any sequence which is effective to meet the objectives there intended, unless the context specifically indicates the contrary.

(37) The patents, papers, and book excerpts cited above are hereby incorporated herein by reference in in their entireties.