Method for Preparing Natural Antibacterial Agent by Immobilizing Plant Essential Oil in Attapulgite Pore Channels

Abstract

A method for preparing a natural antibacterial agent by utilizing attapulgite pore channels to immobilize plant essential oil. Natural antibacterial agent plant essential oil is sprayed on the surface of attapulgite powder while stirring, after mechanically grinding the materials, a liquid glycerol monolaurate and quaternary ammonium salt aqueous solution are sequentially sprayed, after uniformly stirring and mixing the materials, a mixture is extruded by using an extruder, and drying, crushing and packaging are performed.

Claims

1. A method for preparing a natural antibacterial agent by immobilizing a plant essential oil in attapulgite pore channels, including: spraying a natural antibacterial agent of plant essential oil on a surface of attapulgite powders under stirring, mechanically grinding, then spraying liquid glyceryl monolaurate and a quaternary ammonium salt aqueous solution in turn, followed by fully stirring, and extruding with an extruder, drying, crushing and packaging.

2. The method according to claim 1, wherein the attapulgite is a product with purity equal to or greater than 90% obtained by acidification with sulfuric acid accounting for a mass percentage of 2-3% of the attapulgite to remove sands.

3. The method according to claim 1, wherein the natural antibacterial agent of plant essential oil is one or a mixture of at least two selected from the group consisting of oregano essential oil, carvacrol, cinnamaldehyde, citral, tea tree oil, eucalyptus oil and wormwood oil; an amount of the natural antibacterial agent of plant essential oil accounts for a mass percentage of 15-50% of the attapulgite.

4. The method according to claim 1, wherein the mechanical grinding is conducted by grinding for 30 min in a ball mill with a ball-to-material ratio of 1:10.

5. The method according to claim 1, wherein an amount of glyceryl monolaurate accounts for a mass percentage of 5-10% of the attapulgite.

6. The method according to claim 1, wherein the quaternary ammonium salt aqueous solution is any one selected from the group consisting of a cetyltrimethylammonium bromide-chitooligosaccharide aqueous solution, a carboxymethyl hydroxypropyltrimethylammonium chloride-chitooligosacchari de aqueous solution, an N-hydroxypropyltrimethylammonium chloride-chitooligosaccharide aqueous solution, a cetyltrimethylammonium bromide aqueous solution, and a benzyldodecyldimethylammonium bromide aqueous solution.

7. The method according to claim 6, wherein an amount of the quaternary ammonium salt accounts for a mass percentage of 1-5% of the attapulgite.

8. The method according to claim 1, wherein the extrusion is performed by extruding a mixture into long strips in semi-dry state with the extruder, and a diameter of the long strip is in a range of 0.2-0.6 mm.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0021] FIG. 1 is a digital photograph of an extruded product according to the present disclosure.

[0022] FIG. 2 shows the results of the MIC assay of samples prepared according to the present disclosure against E. coli and S. aureus.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] The method for preparing a natural antibacterial agent by immobilizing a plant essential oil in attapulgite and antibacterial property according to the present disclosure will be described in detail below with reference to embodiments.

EXAMPLE 1

[0024] Under stirring, a mixture of oregano essential oil accounting for a mass percentage of 5% of the attapulgite and carvacrol accounting for a mass percentage of 10% of the attapulgite was sprayed onto the surface of attapulgite powders, and then placed in a ball mill for grinding for 30 min with a ball-to-material ratio of 1:10. The resulting mixture was placed in a stirring tank, sprayed with heating-melted glyceryl monolaurate accounting for a mass percentage of 5% of the attapulgite, and then sprayed with a cetyltrimethylammonium bromide-chitooligosaccharide aqueous solution accounting for a mass percentage of 1% of the attapulgite. After spraying, the resultant was continuously stirred for 30 min, and then put into an extruder for extrusion. The extrudate was dried, crushed and packaged to obtain a product.

EXAMPLE 2

[0025] Under stirring, carvacrol accounting for a mass percentage of 50% of the attapulgite was sprayed onto the surface of attapulgite powders, and then placed in a ball mill for grinding for 30 min with a ball-to-material ratio of 1:10. The resulting mixture was placed in a stirring tank, sprayed with heating-melted glyceryl monolaurate accounting for a mass percentage of 10% of the attapulgite, and then sprayed with a cetyltrimethylammonium bromide-chitooligosaccharide aqueous solution accounting for a mass percentage of 5% of the attapulgite. After spraying, the resultant was continuously stirred for 30 min, and then put into an extruder for extrusion. The extrudate was dried, crushed and packaged to obtain a product.

EXAMPLE 3

[0026] Under stirring, a mixture of oregano essential oil accounting for a mass percentage of 5% of the attapulgite, carvacrol accounting for a mass percentage of 5% of the attapulgite, cinnamaldehyde accounting for a mass percentage of 5% of the attapulgite and citral accounting for a mass percentage of 5% of the attapulgite was sprayed onto the surface of attapulgite powders, and then placed in a ball mill for grinding for 30 min with a ball-to-material ratio of 1:10. The resulting mixture was placed in a stirring tank, sprayed with heating-melted glyceryl monolaurate accounting for a mass percentage of 7.5% of the attapulgite, and then sprayed with a carboxymethyl hydroxypropyltrimethylammonium chloride-chitooligosacchari de aqueous solution accounting for a mass percentage of 2.5% of the attapulgite. After spraying, the resultant was continuously stirred for 30 min, and then put into an extruder for extrusion. The extrudate was dried, crushed and packaged to obtain a product.

EXAMPLE 4

[0027] Under stirring, cinnamaldehyde accounting for a mass percentage of 30% of the attapulgite was sprayed onto the surface of attapulgite powders, and then placed in a ball mill for grinding for 30 min with a ball-to-material ratio of 1:10. The resulting mixture was placed in a stirring tank, sprayed with heating-melted glyceryl monolaurate accounting for a mass percentage of 5% of the attapulgite, and then sprayed with an N-hydroxypropyltrimethylammonium chloride-chitooligosaccharide aqueous solution accounting for a mass percentage of 2.5% of the attapulgite. After spraying, the resultant was continuously stirred for 30 min, and then put into an extruder for extrusion. The extrudate was dried, crushed and packaged to obtain a product.

EXAMPLE 5

[0028] Under stirring, a mixture of carvacrol accounting for a mass percentage of 5% of the attapulgite, cinnamaldehyde accounting for a mass percentage of 10% of the attapulgite and tea tree oil accounting for a mass percentage of 5% of the attapulgite was sprayed onto the surface of attapulgite powders, and then placed in a ball mill for grinding for 30 min with a ball-to-material ratio of 1:10. The resulting mixture was placed in a stirring tank, sprayed with heating-melted glyceryl monolaurate accounting for a mass percentage of 8% of the attapulgite, and then sprayed with a benzyldodecyldimethylammonium bromide aqueous solution accounting for a mass percentage of 5% of the attapulgite. After spraying, the resultant was continuously stirred for 30 min, and then put into an extruder for extrusion. The extrudate was dried, crushed and packaged to obtain a product.

EXAMPLE 6

[0029] Under stirring, a mixture of oregano essential oil accounting for a mass percentage of 2.5% of the attapulgite, carvacrol accounting for a mass percentage of 5% of the attapulgite, cinnamaldehyde accounting for a mass percentage of 5% of the attapulgite, citral accounting for a mass percentage of 2.5% of the attapulgite, tea tree oil accounting for a mass percentage of 2.5% of the attapulgite and wormwood oil accounting for a mass percentage of 2.5% of the attapulgite was sprayed onto the surface of attapulgite powders, and then placed in a ball mill for grinding for 30 min with a ball-to-material ratio of 1:10. The resulting mixture was placed in a stirring tank, sprayed with heating-melted glyceryl monolaurate accounting for a mass percentage of 3% of the attapulgite, and then sprayed with an N-hydroxypropyltrimethylammonium chloride-chitooligosaccharide aqueous solution accounting for a mass percentage of 3% of the attapulgite. After spraying, the resultant was continuously stirred for 30 min, and then put into an extruder for extrusion. The extrudate was dried, crushed and packaged to obtain a product.

[0030] In the above examples, the attapulgite was a product with purity equal to or greater than 90%, which was obtained by acidification with sulfuric acid accounting for a mass percentage of 2-3% of the attapulgite to remove the sands.