COMPOSITION CONTAINING CANNABINOIDS

Abstract

Self-emulsifying compositions are disclosed that form stable monodispersions in aqueous solutions containing relatively high concentrations of cannabinoids. They can be easily diluted in aqueous solutions. This makes them suitable for the production of water-based oral products, in particular pharmaceutical compositions, medical devices or food products.

Claims

1. A composition containing a cannabinoid or cannabinoid extract, a surfactant and two different lipid fractions.

2. The composition of claim 1, characterized in that the cannabinoid or cannabinoid extract constitutes no more than 20% by weight of the composition, the surfactant constitutes 30-50% by weight of the composition, the first lipid fraction constitutes 20-35% by weight of the composition, the second lipid fraction constitutes 15-30% by weight of the composition.

3. The composition of claim 1, characterized in that the cannabinoid is a compound selected from the group consisting of: Δ.sup.9-tetrahydrocannabinol (Δ.sup.9-THC), cannabidiol (CBD), tetrahydrocannabinolic acid (THC-A), cannabidiolic acid (CBDA), cannabinol (CBN), cannabigerol (CBG), cannabigerolic acid (CBG-A), cannabichromene (CBC), tetrahydrocannabivarin (THC-V), Δ.sup.8-tetrahydrocannabinol (Δ.sup.8-THC), cannabidivarin (CBDV) or cannabicycol (CBL), or mixtures thereof.

4. The composition of claim 1, characterized in that the cannabinoid extract is an extract of Cannabis sativa, Cannabis indica, Cannabis hybrid or another species of the genus Cannabis or mixtures thereof.

5. The composition of claim 1, characterized in that the surfactant is polysorbate 80, the first lipid fraction is a C8÷C12 medium chain length triacylglyceride and the second lipid fraction is a C8÷C12 medium chain mono-diacylglyceride.

6. The composition of claim 1, characterized in that it further comprises a substance selected from the group consisting of: water and preserving, sweetening, colouring or pH-stabilizing additives suitable for use in pharmaceutical or food products.

7. Use of the composition of claim 1 for preparation of a water-based oral product, preferably a pharmaceutical composition, a medical product or a food product.

8. A method for production of a stable monodisperse system, characterized in that water or an aqueous solution is mixed with the composition as defined in claim 1.

9. The method claim 8, characterized in that the mixing time is less than 120 seconds, preferably less than 60 seconds, with mixing intensity not exceeding 2500 RPM, preferably with mixing intensity not exceeding 150 RPM.

10. A monodisperse system, characterized in that it consists of a dispersion medium being water or an aqueous solution and a dispersed phase formed by the particles obtained from the composition as defined in claim 1, wherein the particles preferably have an outer layer containing a surfactant, and an inner layer which is a mixture of lipids in which a cannabinoid or cannabinoid extract is dissolved.

11. The monodisperse system of claim 10, characterized in that the dispersed phase is formed by particles smaller than 180 nm.

12. The monodisperse system of claim 10, characterized in that it has a transparency of more than 85%, preferably more than 90%.

13. The monodisperse system of claim 10, characterized in that it has a monodispersity below 0.250.

14. The monodisperse system of claim 10, characterized in that it maintains the nanoemulsion stability upon dilution up to 10,000× with water or an aqueous solution.

15. The monodisperse system of claim 10, characterized in that it maintains the nanoemulsion stability over the pH range from 8 to 3.

Description

GENERAL PROCEDURE FOR THE PREPARATION OF THE COMPOSITIONS OF THE INVENTION

[0069] All ingredients of the composition were weighed out into a common vessel. The contents of the vessel were mixed for 30 min at room temperature. Alternatively, the entire mixture may be heated to accelerate the dissolution of the ingredients and then mixed to homogenize the mixture.

[0070] Example Procedure for Preparing a Composition Containing 10% by Weight of CBD:

[0071] The composition was prepared by weighing out into one vessel 0.460 mg of surfactant (T80), 220 mg of medium chain triacylglyceride (MCT), 220 mg of medium chain mono-diacylglyceride (MCM) and 100 mg of cannabinoid extract (CBD). The mixture was mixed for 30 min with a stir bar at 300 RPM until uniform at room temperature, or for a shorter time at higher temperature (e.g. 60° C.).

[0072] Procedure for the Preparation of Nanoemulsions.

[0073] Basic dilution—hundredfold (100×) was obtained by introducing 100 microliters (4) of the finished composition by dropping, in a short time (<10 seconds), into a glass vial with a stirring bar containing 10 millilitres (mL) of water. The contents of the glass vial were mixed with rotation up to 150 RPM. The dispersion time was measured until a homogeneous system was obtained.

[0074] A 200-fold (200×) dilution was obtained by diluting 1:1 (v/v) the previously prepared nanoemulsion of the 100-fold dilution of the composition with water.

[0075] Methodology of Conducted Research [0076] dispersion time was measured in seconds (t, sec) from the moment of adding the last drop of the mixture to water until complete dissolution, [0077] transmittance of nanoemulsions was measured in percent (% T) at a wavelength of λ=600 nm using a Merck® spectrometer model Pharo 300, [0078] hydrodynamic diameter of the nanoparticles was given in nanometers (D.sub.H, nm) and measured using a Zetasizer Nano-ZS device from Malvern, [0079] polydispersity of the particles after dispersion in water (polydispersity index) was expressed as a PdI value and measured using a Zetasizer Nano-ZS device from Malvern.

[0080] Development of a Self-Emulsifying Cannabinoid Composition of the Invention

[0081] The composition that is the subject of the invention contains 4 components:

TABLE-US-00002 1 2 3 4 Surfactant Lipid 1 Lipid 2 Cannabinoid
wherein: [0082] The surfactant (T80) is a polyoxyethylene derivative of sorbitan and oleic acid (Polysorbate 80, Polyoxyethylene Sorbitan Monooleate, Tween™ 80), commercially available and offered by many manufacturers. Tween™ 80 was used in the experiment. [0083] The Lipid 1 (MCT) is a medium chain triglyceride (C8-012 acid mixture) commercially available and offered by many manufacturers. Crodamol™ GTCC was used in the experiment. [0084] The Lipid 2 (MCM) is a mono-diglyceride of medium chain fatty acids, mainly caprylic (C8) and capric (decanoic, 010), commercially available and offered by many manufacturers. 101 OLEOCHEMICAL—Imwitor® 988 was used in the experiment. [0085] The cannabinoid (CBD), possibly as a component of a cannabinoid extract, means extracts and isolates that are commercially available products and are offered by many manufacturers. In the application, most of the testing was performed using cannabidiol, 95% purity isolate purchased from Kannastar.

[0086] The content of the composition and the proportion of ingredients are % by weight (wt./wt./wt./wt.). The embodiments of the compositions of the invention refer to the following content ranges of the individual components: [0087] T80: 30-50%, [0088] MCT: 20-35%, [0089] MCM: 15-30%, [0090] CBD: 0-20%.

TABLE-US-00003 TABLE 2 Embodiments of the compositions of the invention T80 MCT MCM CBD Homogeneous Dispersion Transmittance Transmittance D.sub.H No. % % % % system time [sec] [%] 100× [%] 200×* [nm] Pdl 19 37.0 35.0 20.1 7.9 YES 20 36.1% 58.8% 113.3 0.219 20 30.0 20.0 30.0 20.0 YES 29 0.13% 0.86% 154.1 0.136 21 36.1 24.6 19.3 20.0 YES 67 0.12% 0.59% 200.0 0.278 22 36.1 24.6 19.3 20.0 YES 70 0.12% 0.44% 190.9 0.254 23 30.0 30.0 25.0 15.0 YES 10 0.82% 8.68% 188.6 0.256 24 36.3 20.0 28.9 14.8 YES 15 20.4% 44.6% 135.6 0.246 25 30.0 30.0 25.0 15.0 YES 30 0.43% 5.87% 150.3 0.147 26 30.0 35.0 15.0 20.0 YES 86 0.11% 0.13% 262.0 0.472 27 42.1 27.7 22.7 7.5 YES 20 .sup. 76% 86.1% 71.3 0.209 28 44.1 20.0 30.0 5.9 YES 30 96.3% 97.9% 32.5 0.205 29 42.7 20.0 24.7 12.5 YES 15 74.3% 85.9% 66.3 0.177 30 30.9 35.0 30.0 4.1 YES 15 35.2% 58.9% 115.7 0.188 31 37.2 24.8 30.0 8.0 YES 15 59.6% 77.1% 104.0 0.274 32 43.1 30.0 15.0 11.9 YES 50 31.3% 55.5% 106.2 0.143 33 42.2 31.1 26.7 0.0 YES 22 91.9% 95.9% 50.9 0.238 34 48.3 20.0 15.0 16.7 YES 80 15.4% 38.9% 124.2 0.214 35 42.2 31.1 26.7 0.0 YES 30 86.3% .sup. 93% 62.6 0.255 36 50.0 35.0 15.0 0.0 YES 250 4.91% .sup. 22% 266.5 0.293 37 50.0 25.2 20.2 4.6 YES 30 .sup. 95% 97.3% 35.8 0.194 38 36.4 34.3 15.0 14.3 YES 70  0.2% 3.07% 265.3 0.266 39 50.0 20.0 30.0 0.0 YES 25 98.7% 99.5% 23.8 0.198 40 37.0 35.0 20.1 7.9 YES 25 .sup. 11% .sup. 33% 139.5 0.217 41 37.2 24.8 30.0 8.0 YES 20 57.3% 75.3% 90.5 0.186 42 50.0 24.4 15.0 10.6 YES 50 77.9% 87.7% 69.6 0.193 *200-fold (200×) dilution was performed as a control parameter for the quality of nanoemulsions and the performance of self-emulsifying compositions in order to better characterize the systems by transmittance (% T).

[0091] Study of Selected Examples of Compositions

[0092] Examples of compositions with different contents of cannabinoid extracts were also studied.

[0093] The following systems have been found as particularly preferable for the different formulation applications (T80:MCT:MCM:CBD, wt./wt./wt./wt.) as listed below: [0094] 0% CBD (44.6:25.4:30.0:0), [0095] 5% CBD (44.3:26.9:23.7:5), [0096] 10% CBD (46.0:22.0:22.0:10), [0097] 15% CBD (43.7:20.0:21.3:15), [0098] 20% CBD (37.3:20.0:22.7:20).

TABLE-US-00004 TABLE 3 Properties of compositions with different contents of cannabinoid extracts. Dispersion Transmittance Transmittance D.sub.H # Name time [sec] [%] 100× [%] 200×* [nm] Pdl 1 0% CBD 15 98.1% 99.1% 23.9 0.162 2 0% CBD 28 97.2% 99.4% 24.0 0.146 3 0% CBD 25 97.9% 99.5% 22.8 0.103 4 5% CBD 29 94.5% 97.3% 37.7 0.177 5 5% CBD 29 94.3% 96.9% 38.0 0.192 6 5% CBD 27 95.3% 96.6% 39.2 0.205 7 10% CBD 27 93.2% 95.0% 46.6 0.175 8 10% CBD 23 92.2% 95.3% 44.6 0.168 9 10% CBD 27 91.3% 95.1% 45.6 0.179 10 15% CBD 39 50.6% 71.1% 86.5 0.181 11 15% CBD 44 51.6% 70.9% 95.5 0.229 12 15% CBD 63 52.8% 71.4% 87.3 0.172 13 20% CBD 62 0.4% 4.7% 148.9 0.198 14 20% CBD 52 0.2% 2.6% 154.1 0.166 15 20% CBD 72 0.2% 1.6% 153.2 0.147 Average results (n = 3) Dispersion Transmittance Transmittance D.sub.H No. Name time [sec] [%] 100× [%] 200×* [nm] Pdl 43 0% CBD 23 ± 7 97.7 ± 0.5% 99.3 ± 0.2% 23.6 ± 0.7 0.137 ± 0.031 44 5% CBD 28 ± 1 94.7 ± 0.5% 96.9 ± 0.4% 38.3 ± 0.8 0.191 ± 0.014 45 10% CBD 26 ± 2 92.2 ± 1.0% 95.1 ± 0.2% 45.6 ± 1.0 0.174 ± 0.006 46 15% CBD  49 ± 13 51.7 ± 1.1% 71.1 ± 0.3% 89.8 ± 5.0 0.194 ± 0.031 47 20% CBD  62 ± 10  0.3 ± 0.1%  3.0 ± 1.6% 152.1 ± 2.8  0.170 ± 0.026

[0099] All variants of the self-emulsifying compositions and dispersions systems were prepared in triplicate and homogeneous samples were obtained.

[0100] The study, the results of which are presented in Table 3 (above), demonstrated that the dispersion time for the composition (43-46) did not exceed 60 seconds, while with the 20% content of cannabinoids for the sample (47), the dispersion time was just over 60 seconds. The transmittance above 90% was achieved for the composition containing up to 10% of CBD (45), but at higher CBD concentrations, i.e. above 10% (46-47), the minimum transmittance value, i.e. 85%, was not reached; all compositions presented in Table 3 are homogeneous and have very low PdI values <0.200, which proves a very good quality of nanoemulsions (high emulsion stability); the obtained hydrodynamic diameter of nanoparticles is very low for all compositions and is in the range of D.sub.H<180 nm; moreover, up to 15% CBD content (inclusive) it is less than 100 nm.

[0101] Study of the Compositions Using a Variety of Cannabinoids.

[0102] A significant advantage of the self-emulsifying compositions of the invention is that these compositions are universal and perform well with various hydrophobic compounds, therefore experiments have been carried out using a variety of cannabinoids. Variants of compositions were tested, whereas cannabinoids were used as pure compounds, i.e. cannabidiol (CBD), tetrahydrocannabinol (THC), a mixture of various naturally occurring cannabinoids (full spectrum, abbreviated as “MIX”), various extracts with lower cannabinoid content (designated as “Extracts type . . . ”), as well as hemp oil with a trace amount of cannabinoids and characterized by a high content of unsaturated fatty acids.

[0103] All compositions were based on one, recognized as the model, self-emulsifying composition containing 10% of the raw material content—cannabinoid. (T80:MCT:MCM:Cannabinoid, wt./wt./wt./wt.).

[0104] The research was conducted with the use of: [0105] CBD—isolate with a purity of ≥95%, [0106] THC—isolate with a purity of ≥95%, [0107] MIX—a mixture of naturally occurring cannabinoids and phytosterols, terpenes, cannabinoid content approx. 95%, [0108] Extract type 1—a mixture of naturally occurring cannabinoids with a content of 70.66% CBD, 4.36% THC, 2.18% CBG, [0109] Extract type 2—a mixture of naturally occurring cannabinoids with a content of 29.79% CBDA, 24.31% CBD, 1.26% THC, 0.60% THCA, [0110] Extract type 3—a mixture of naturally occurring cannabinoids with a content of 25.17% CBD, 18.79% CBDA, 1.30% THC, 0.14% THCA, 1.84% CBC, 0.89% CBG, 0.49% CBGA, 0.22% CBDV, 0.05% CBL, 0.04% CBN, 0.04% delta-8-THC, [0111] Hemp oil—oil obtained from hemp seeds (Cannabis sativia) containing approx. 54% of linoleic acid, 15% of α-linolenic acid, 4% of Y-linolenic acid, 13% of oleic acid and 14% of other fatty acids.

TABLE-US-00005 TABLE 4 Properties of compositions using various cannabinoids. Dispersion Transmittance Transmittance D.sub.H # Name time [sec] [%] 100× [%] 200×* [nm] Pdl 1 10% MIX 28 90.9% 93.6% 49.0 0.184 2 10% MIX 23 86.4% 92.2% 52.7 0.226 3 10% MIX 27 84.6% 92.0% 51.3 0.200 4 10% THC 32 95.5% 97.7% 31.0 0.183 5 10% THC 20 96.9% 98.5% 32.5 0.223 6 10% THC 38 96.6% 98.0% 32.9 0.235 7 10% Extract 19 96.1% 97.4% 33.4 0.234 type 1 8 10% Extract 19 95.9% 98.2% 34.3 0.224 type 1 9 10% Extract 30 95.3% 96.9% 34.8 0.231 type 1 10 10% Extract 19 94.4% 99.8% 23.2 0.207 type 2 11 10% Extract 20 94.3% 100.3% 22.9 0.238 type 2 12 10% Extract 23 93.1% 100.5% 22.1 0.202 type 2 13 10% Extract 22 89.9% 98.6% 37.9 0.174 type 3 14 10% Extract 23 93.1% 96.2% 37.2 0.179 type 3 15 10% Extract 13.5 93.2% 98.1% 37.2 0.183 type 3 16 10% Hemp oil 35 90.6% 94.4% 62.0 0.284 17 10% Hemp oil 33 92.7% 95.6% 54.5 0.283 18 10% Hemp oil 28 90.9% 95.0% 59.7 0.276 Average results (n = 3) Dispersion Transmittance Transmittance D.sub.H No. Name time [sec] [%] 100× [%] 200×* [nm] Pdl 45 CBD 26 ± 2 92.2 ± 1.0% 95.1 ± 0.2% 45.6 ± 1.0 0.174 ± 0.006 48 MIX 26 ± 3 87.3 ± 3.2% 92.6 ± 0.9% 51.0 ± 1.9 0.203 ± 0.021 49 THC 30 ± 9 96.3 ± 0.7% 98.1 ± 0.4% 32.1 ± 1.0 0.214 ± 0.027 50 Extract type 1 23 ± 6 95.8 ± 0.4% 97.5 ± 0.7% 34.2 ± 0.7 0.230 ± 0.005 51 Extract type 2 21 ± 2 93.9 ± 0.7% 100.2 ± 0.4%  22.7 ± 0.6 0.216 ± 0.020 52 Extract type 3 20 ± 5 92.1 ± 1.9% 97.6 ± 1.3% 37.4 ± 0.4 0.179 ± 0.005 53 Hemp oil 32 ± 4 91.1 ± 1.1% 95.0 ± 0.6% 58.7 ± 3.8 0.281 ± 0.004

[0112] The conducted study demonstrates that the dispersion time for all formulations (45, 48÷53) did not exceed 60 s, the self-emulsifying compositions have similar characteristics regardless of the hemp raw material and the degree of its purification, transmittance for 100× (100-fold) dilution in water in most cases exceeded 90%, with the exception of raw material containing full spectrum of cannabinoids, as well as terpenes and phytosterols (48), the presence of which slightly reduced the transmittance to the value of 87%, all compositions, regardless of the raw material used, had a small nanoparticle hydrodynamic diameter of D.sub.H<60 nm. All compositions, regardless of the raw material used, were monodisperse maintaining PdI<0.250 with the exception of sample 53 which had PdI value below 0.300.

[0113] Study on the Selection of the Surfactant Contained in the Composition. [0114] The properties of variants of the compositions containing the above-defined particularly preferable proportions of the remaining ingredients determined for 10% CBD model formulation, in which various surfactants (surfactant:MCT:MCM:CBD, wt./wt./wt./wt.) were used, was also investigated.

[0115] The study was carried out with the use of the following surfactants from appropriate groups: [0116] monoacylated derivatives of polyoxyethylene sorbitan—there are many different derivatives of this class of surfactants, for which the abbreviation Tween™ is commonly used and depending on the acyl residue of the respective fatty acid, Tween 20, 40, 60, 80 and 85 are distinguished. In the application, two of them were used in addition to Tween™ 80: [0117] polyoxyethylene sorbitan monolaurate as “Tween 20” (Polysorbate 20, Polyoxyethylene 20 sorbitan monolaurate), commercially available and offered by many manufacturers. Tween™ 20 from CRODA was used in the experiment; [0118] polyoxyethylene sorbitan monostearate as “Tween 60” (Polysorbate 60, Polyoxyethylene 60 sorbitan monostearate), commercially available and offered by many manufacturers. Tween™ 60 from CRODA was used in the experiment; [0119] ethoxylated oil derivatives as “Etocas”—a surfactant based on polyoxyethylene (35) castor oil (PEG-35 castor oil, Polyoxyl 35 Castor Oil), commercially available and offered by many manufacturers. Etocas™ 35 from CRODA was used in the experiment; [0120] hydrogenated ethoxylated derivatives of oils as “Croduret”—a surfactant based on polyoxyl 40 hydrogenated castor oil (PEG-40 hydrogenated castor oil), commercially available and offered by many manufacturers. Croduret™ 50 from CRODA was used in the experiment; [0121] acyl polyglyceryl derivatives, as “Plurol”—polyglyceryl-3 dioleate, commercially available surfactants offered by many manufacturers. Plurol® Oleique from GATTEFOSE was used in the experiment.

TABLE-US-00006 TABLE 5 Surfactant selection study for the self-emulsifying composition. Dispersion Transmittance Transmittance D.sub.H # Name time [sec] [%] 100× [%] 200×* [nm] Pdl 1 Tween 20 44 29.7% 65.3% 110.8 0.133 2 Tween 20 55 36.3% 58.3% 106.8 0.131 3 Tween 20 80 40.2% 61.3% 109.0 0.155 4 Tween 60 58 56.3% 76.5% 97.2 0.203 5 Tween 60 32 63.5% 81.0% 95.3 0.211 6 Tween 60 28 56.4% 76.0% 118.2 0.266 7 Etocas 515 95.8% 100.7% 98.7 0.188 8 Etocas 420 95.8% 101.1% 28.5 0.169 9 Etocas 305 96.5% 101.6% 37.4 0.237 10 Croduret 548 96.3% 100.8% 27.1 0.081 11 Croduret 607 97.1% 98.9% 28.0 0.101 12 Croduret 364 98.1% 101.8% 28.5 0.100 13 Plurol heterogeneous n/d n/d n/d n/d 14 Plurol system 15 Plurol Average results (n = 3) Dispersion Transmittance Transmittance D.sub.H No. Name time [sec] [%] 100× [%] 200×* [nm] Pdl 45 Tween 80 26 ± 2  92.2 ± 1.0% 95.1 ± 0.2% 45.6 ± 1.0 0.174 ± 0.006 54 Tween 20 60 ± 18 35.4 ± 5.3% 61.6 ± 3.5% 108.9 ± 2.0  0.140 ± 0.013 55 Tween 60 39 ± 16 58.7 ± 4.1% 77.8 ± 2.8% 103.6 ± 12.7 0.227 ± 0.034 56 Etocas 35 413 ± 105 96.0 ± 0.4% 101.1 ± 0.5%   54.9 ± 38.2 0.198 ± 0.035 57 Croduret 506 ± 127 97.2 ± 0.9% 100.5 ± 1.5%  27.9 ± 0.7 0.094 ± 0.011 58 Plurol n/d n/d n/d n/d n/d n/d—not determined (due to the sample heterogeneity and/or the polydisperse sample not suitable for testing).

[0122] The results of the study show that the compositions containing Tween™ 80 (45) have the best dispersion time, very good nanoparticle size parameters, are characterized by monodispersity, while the alternative Tween™ 60 (55) is characterized by much lower transmittance, and the compositions containing Etocas (56) and Croduret (57) are characterized by very high transmittance, while they disperse worse and longer than the composition containing Tween™ 80 (45).

[0123] Therefore, in the preferred embodiment of the compositions of the invention, Tween™ 80 is used as the surfactant.

[0124] Study on the Selection of the Lipid Component (Lipid 1) Contained in the Composition.

[0125] A parallel study was also carried out on the selection of the lipid component Lipid 1, which in the preferred composition of the invention is a medium-chain triacylglyceride (MCT) with a mixture of acids of C8÷C12 length. Medium-chain triacylglycerides are predominantly esters with caprylic (octanoic, C8:0) and capric (decanoic, 010:0) acids. MCTs are commercially available and offered by many manufacturers under various trade names, including: Crodamol GTCC triacylglyceride from CRODA, Labrafac lipophile from GATEFOSSE, Miglyol 808 from 101 OLEOCHEMICAL, Miglyol 812 N from 101 OLEOCHEMICAL, Imwitor® 928 from 101 OLEOCHEMICAL, Captex® 300 from ABITEC or Captex® 355 from ABITEC. The first two were tested, forming compositions identical in terms of parameters.

[0126] The formulations containing the preferred ingredient ratios specified above for 10% CBD model formulation content with different lipids instead of MCT (T80:Lipid 1:MCM:CBD, wt./wt./wt./wt.) were also investigated.

[0127] The following groups of compounds were used in the study as the lipid component Lipid 1: [0128] hemp oil—oil obtained from hemp seeds (Cannabis sativia) containing approximately 54% of linoleic acid, 15% of α-linolenic acid, 4% of γ-linolenic acid, 13% of oleic acid and 14% of other fatty acids. The compound used is classified as a long chain triacylglyceride (LCT) with a mixture of C14÷C26 acids, [0129] fatty acid as “FA”—oleic acid was chosen as an example of this group of compounds, which is an example of a long-chain free fatty acid, which has 18 carbon atoms and one double bond (018:1). Fatty acids are commercially available and offered by many manufacturers. Oleic acid with a purity of >95% purchased at Sigma-Aldrich was used in the experiment. [0130] triacylglyceride as “TAG”—natural oil was chosen as an example of this group of compounds, which mainly contains various triacylglycerides composed mostly of oleic acid derivatives, but also palmitic acid, linoleic acid and other fatty acids. A commercial food grade oil, sold as olive oil, which also contains small amounts (<1%) of squalene, phenolic compounds and vitamin E, was used in the experiment.

TABLE-US-00007 TABLE 6 Lipid component (Lipid 1) selection study for the self-emulsifying composition. Dispersion time Transmittance Transmittance D.sub.H # Name [sec] [%] 100× [%] 200×* [nm] Pdl 1 hemp oil 14 55.2% 74.1% 53.4 0.387 2 hemp oil 21 69.1% 80.1% 50.4 0.271 3 hemp oil 21 71.2% 83.5% 54.4 0.301 4 FA heterogeneous n/d n/d n/d n/d 5 FA system 6 FA 7 TAG heterogeneous n/d n/d n/d n/d 8 TAG system 9 TAG Average results (n = 3) Dispersion Transmittance Transmittance D.sub.H Nr Name time [sec] [%] 100× [%] 200×* [nm] Pdl 45 MCT 26 ± 2 92.2 ± 1.0% 95.1 ± 0.2%  45.6 ± 1.0 0.174 ± 0.006 59 hemp oil 19 ± 4 65.2 ± 8.7% 79.2 ± 4.8% 52.75 ± 2.1 0.320 ± 0.060 60 FA n/d n/d n/d n/d n/d 61 TAG n/d n/d n/d n/d n/d n/d—not determined (due to the sample heterogeneity and/or the polydisperse sample not suitable for testing).

[0131] The conducted study demonstrates that the compositions containing MCT (45) as the Lipid 1 are the best variant, because they are characterized by a very short dispersion time and high transparency assessed by transmittance measurement. In the case of the variant using hemp oil (59), the system has a lower transmittance and almost twice higher polydispersity coefficient comparing to the optimal composition (45). In the preferred composition of the invention, Lipid 1 is MCT.

[0132] Study on the Selection of the Lipid Component (Lipid 2) of the Self-Emulsifying Composition.

[0133] A study on the selection of the lipid component Lipid 2, which in the preferred composition of the invention is medium-chain mono-diacylglyceride (MCM) with a mixture of acids of C8÷C12 length was also carried out. Medium-chain mono-diacylglycerides are commercially available and offered by many manufacturers under various trade names, including: Imwitor® 988 (Type I) from 101 OLEOCHEMICAL, Imwitor® 308 (Type II) from 101 OLEOCHEMICAL, Imwitor® 742 (Type I) from 101 OLEOCHEMICAL, Imwitor® 928 from 101 OLEOCHEMICAL, Campul MCM EP/NF (Type I) from ABITEC, Campul 808G EP/NF (Type II) from ABITEC, Campul MCM-8 (Type I) from ABITEC, or Campul MCM NF (Type I) from ABITEC.

TABLE-US-00008 TABLE 7 Comparison study for MCM of various manufacturers and types as the lipid component Lipid 2. Dispersion Transmittance Transmittance D.sub.H # Name time [sec] [%] 100× [%] 200×* [nm] Pdl 1 Imwitor ® 988 27 93.2% 95.0% 46.6 0.175 2 Imwitor ® 988 23 92.2% 95.3% 44.6 0.168 3 Imwitor ® 988 27 91.3% 95.1% 45.6 0.179 4 Campul 808G 17 95.7% 97.2% 27.6 0.205 5 Campul 808G 18 97.0% 98.5% 27.9 0.182 6 Campul 808G 19 97.2% 97.9% 27.9 0.183 7 Capmul MCM 25 94.5% 96.8% 37.5 0.258 EP/NE 8 Capmul MCM 20 95.8% 97.2% 30.5 0.155 EP/NE 9 Capmul MCM 37 94.9% 96.9% 34.9 0.174 EP/NE Average results (n = 3) Dispersion Transmittance Transmittance D.sub.H No. Name time [sec] [%] 100× [%] 200×* [nm] Pdl 45 Imwitor ® 988 26 ± 2 92.2 ± 1.0% 95.10 ± 0.20% 45.60 ± 1.0 0.174 ± 0.006 62 Capmul 808G 18 ± 1 96.6 ± 0.8% 97.87 ± 0.65% 27.80 ± 0.2 0.190 ± 0.013 63 Capmul MCM 27 ± 9 95.1 ± 0.7% 96.97 ± 0.21% 34.29 ± 3.5 0.196 ± 0.055 EP/NE

[0134] Based on Table 7, the compositions containing MCM from different manufacturers as Lipid 2, identical in terms of the contents, form identical nanoparticle systems and are characterized by: similar dispersion time, transmittance, hydrodynamic diameter, and they form monodisperse systems with a similar PdI parameter of just under 0.200.

[0135] The formulations containing the preferred ingredient ratios specified above for 10% CBD model formulation content with different lipids instead of MCM (T80:MCT:Lipid 2:CBD, wt./wt./wt./wt.) was also investigated. Compounds that are derivatives of MCM from the following groups were used in the study as the lipid component Lipid 2: [0136] medium chain monoesters with propylene glycol—there are several different derivatives of this group of compounds, which are described in the application as “PGM” (propylene glycol monoester). Three of them were tested in the application: [0137] caprylic acid monoester (C8:0) with propylene glycol, type I pharmaceutical classification, abbreviated as “PGMC-I” (propylene glycol monocaprylate, PG monocaprylate C8, Type I, NF) under the trade name Capryol PGMC from GATEFOSSE, [0138] caprylic acid monoester (C8:0) with propylene glycol, type II pharmaceutical classification, abbreviated “PGMC-II” (propylene glycol monocaprylate, PG monocaprylate C8, Type II) under the trade name Capryol 90 from GATEFOSSE, [0139] lauric acid monoester (C12:0) with propylene glycol, type II pharmaceutical classification, abbreviated “PGML” (propylene glycol monolaureate, Type II) under the trade name Capmul PG-12 NEP/NF from ABITEC, [0140] long chain acid monoesters with glycerol—there are several different derivatives of this group of compounds, which are described in the application as “GM” (glycerol mononoester). Three of them were tested in the application: [0141] glycerol monoester with oleic acid (C18:1), abbreviated as “GMO” (glyceryl monooleate, Type 40) under the trade name Peceol from GATEFOSSE, [0142] glycerol monoester with linoleic acid (C18:2), abbreviated as “GML” (glyceryl monolinoleate) under the trade name Maisine CC from GATEFOSSE, [0143] glycerol monoester with ricinoleic acid (C18:1, —OH), abbreviated as “GMR” (glyceryl monoricinoleate) under the trade name Softigen 701 from IOI OLEOCHEMICAL.

TABLE-US-00009 TABLE 8 Lipid component (Lipid 2) selection study for the self-emulsifying composition. Dispersion Transmittance Transmittance D.sub.H # Name time [sec] [%] 100× [%] 200×* [nm] Pdl 1 PGMC-I 520 0.27% 3.75% 179.0 0.221 2 PGMC-I 470 0.28% 4.05% 169.5 0.193 3 PGMC-I 580 0.20% 2.74% 183.6 0.220 4 PGMC-II 195 0.18% 2.16% 208.4 0.293 5 PGMC-II 209 0.34% 4.99% 198.5 0.270 6 PGMC-II 230 0.19% 2.27% 203.9 0.317 7 PGML >1200  8 PGML heterogeneous n/d n/d n/d n/d 9 PGML system 10 GMO >1200  11 GMO heterogeneous n/d n/d n/d n/d 12 GMO system 13 GML >1200  14 GML heterogeneous n/d n/d n/d n/d 15 GML system 16 GMR 140 0.15% 0.66% 233.9 0.426 17 GMR 160 0.13% 0.31% 254.0 0.447 18 GMR 165 0.13% 0.24% 285.7 0.498 Average results (n = 3) Dispersion Transmittance Transmittance D.sub.H No. Name time [sec] [%] 100× [%] 200×* [nm] Pdl 45 MCM 26 ± 2 92.2 ± 1.0%   95.1 ± 0.2%   45.6 ± 1.0 0.174 ± 0.006 64 PGMC-I 523 ± 55 0.25 ± 0.04% 3.51 ± 0.69% 177.37 ± 7.2 0.211 ± 0.016 65 PGMC-II 211 ± 17 0.24 ± 0.09% 3.14 ± 1.60% 203.62 ± 5.0 0.293 ± 0.023 66 PGML n/d n/d n/d n/d n/d 67 GMO n/d n/d n/d n/d n/d 68 GML n/d n/d n/d n/d n/d 69 GMR 155 ± 13 0.14 ± 0.01% 0.40 ± 0.23% 257.88 ± 26.1 0.457 ± 0.037 n/d—not determined (due to the sample heterogeneity and/or the polydisperse sample not suitable for testing).

[0144] Table 8 shows that the compositions based on MCM as Lipid 2 are characterized by a very low dispersion time <60 seconds, a high transparency coefficient assessed by transmittance measurement (% T), very desired nanoparticle size parameters while maintaining monodispersity of the system.

[0145] In a preferred embodiment of the compositions of the invention, MCM is used as Lipid 2.

[0146] Study on the Stability of the Self-Emulsifying Composition During Dilution and Various Dispersion Systems.

[0147] The system stability of the preferred composition with 10% and 20% cannabinoid content was confirmed for dispersion systems diluted up to 10000×. The tests were performed on the basis of two compositions with proportions other than the optimal (variants 1 and 2) to demonstrate the versatility of the technology. The contents of the compositions were adjusted to the range tested in the application and the exact values of the studied compositions are shown in Table 9.

TABLE-US-00010 TABLE 9 Self-emulsifying compositions tested at different dispersion dilutions. Abbre- Surfactant Lipid 1 Lipid 2 Cannabinoid Description viation T80 MCT MCM CBD Optimal system CBD 10% 46% 22% 22% 10% (No. 45) W-1 30% 30% 30% 10% Variant 1 Optimal system CBD 20% 37.3%.sup.  20% 22.7%.sup.  20% (No. 47) W-2 30% 25% 25% 20% Variant 2

[0148] In this part of the study, nanoemulsions were made as described in the general description by introducing an appropriate amount of the tested composition. The mixing speed in this case was 500 RPM in each case. The results did not take into account the dispersion time due to very fast dispersion <10 seconds in each case, while the measurement of transmittance (% T), hydrodynamic diameter and polydispersity were assessed at the appropriate dilution of the system.

[0149] Three dispersion systems were tested: [0150] water (<0.21 uS) with pH=7.01; [0151] an aqueous solution containing 0.8% by weight of citric acid and 0.08% of sodium benzoate, pH=2.61; [0152] an aqueous solution containing 10% by weight of sucrose, 0.8% by weight of citric acid and 0.08% of sodium benzoate, pH=2.74.

[0153] In all tested matrix (water, aqueous solution with the addition of citric acid and sodium benzoate, and aqueous solution with the addition of sugar, citric acid and sodium benzoate), the obtained dispersion system were characterized by very similar parameters, i.e. transparency coefficient assessed by transmittance measurement (% T), nanoparticle hydrodynamic diameter and polydispersity of the system. Table 10 shows only those relating to the use of an aqueous solution containing 0.8% by weight of citric acid and 0.08% of sodium benzoate at pH 2.61 as the dispersion system.

TABLE-US-00011 TABLE 10 Nanoemulsion stability study for different dilutions in aqueous solution at pH = 2.61. Transmittance D.sub.H # Name Dilution [%] [nm] Pdl 1 W-1 1000× 1 000 95.5 69.8 0.115 2 W-1 1000× 1 000 95.4 72.4 0.122 3 W-1 1000× 1 000 95.1 70.2 0.105 4 W-1 2500× 2 500 97.7 76.4 0.142 5 W-1 2500× 2 500 97.6 73.6 0.117 6 W-1 2500× 2 500 97.3 75.6 0.121 7 W-1 5000× 5 000 99.1 75 0.126 8 W-1 5000× 5 000 98.9 73.9 0.124 9 W-1 5000× 5 000 99.2 69.6 0.126 10 W-1 10000× 10 000  99.2 75.8 0.142 11 W-1 10000× 10 000  99.2 73.6 0.135 12 W-1 10000× 10 000  99.1 122.1 0.315 13 W-2 1000× 1 000 88.3 97.0 0.108 14 W-2 1000× 1 000 85.1 105.2 0.128 15 W-2 1000× 1 000 87.9 99.9 0.115 16 W-2 2500× 2 500 92.1 104.5 0.137 17 W-2 2500× 2 500 89.6 112.3 0.141 18 W-2 2500× 2 500 90.6 108.5 0.147 19 W-2 5000× 5 000 95.5 102.3 0.137 20 W-2 5000× 5 000 93.4 105.9 0.151 21 W-2 5000× 5 000 91.8 153.1 0.319 22 W-2 10000× 10 000  96.8 125.4 0.263 23 W-2 10000× 10 000  96.1 109.6 0.152 24 W-2 10000× 10 000  95.1 119.7 0.184 Average results (n = 3) Transmittance D.sub.H No. Name Dilution [%] [nm] Pdl 45 CBD 10%   100 92.2 ± 1.0% 45.6 ± 1.0 0.174 ± 0.013 70 W-1 1000× 1 000 95.3 ± 0.2% 70.8 ± 1.4 0.114 ± 0.009 71 W-1 2500× 2 500 97.5 ± 0.2% 75.2 ± 1.4 0.127 ± 0.006 72 W-1 5000× 5 000 99.1 ± 0.2% 72.8 ± 2.9 0.125 ± 0.001 73 W-1 10000× 10 000  99.2 ± 0.1%  90.5 ± 27.4 0.197 ± 0.102 47 CBD 20%   100  0.3 ± 0.1% 152.1 ± 2.8  0.170 ± 0.026 74 W-2 1000× 1 000 87.1 ± 0.7% 100.7 ± 4.2  0.117 ± 0.010 75 W-2 2500× 2 500 90.8 ± 1.3% 108.4 ± 3.9  0.142 ± 0.005 76 W-2 5000× 5 000 93.6 ± 1.9% 120.4 ± 28.3 0.202 ± 0.101 77 W-2 10000× 10 000  96.0 ± 0.9% 118.2 ± 8.0  0.200 ± 0.057

[0154] The compositions of the invention are characterized by dilution stability up to 10,000×. The results summarized in Table 10 show that the tested compositions have a very high transparency factor assessed by transmittance measurement (% T). The dilutions also have no significant effect on the nanoparticle hydrodynamic diameters and the polydispersity of the systems. The results did not include dispersion time due to very fast dispersion <10 seconds.

[0155] Study on the Stability of the Active Substance in the Composition and the Stability Test of the Active Substances Under Simulated Preservation Conditions.

[0156] An important advantage of the self-emulsifying compositions of the invention is that the compositions are used to prepare oral products based on aqueous solutions, especially pharmaceutical compositions, medical compositions or food products. The above-mentioned products must provide delivery of equal doses of the active substance. For this purpose, the variant of the composition for 1000× dilution was verified for the content of the active substance—cannabidiol (CBD) in the fresh emulsion and after a simulated pasteurization test which was carried out by keeping the dispersion system at 72° C. for 15 min. The active substance content was measured using UHPLC instrument Thermo Scientific Dionex Ultimate 3000 UHPLC.sup.+ focused, with Thermo Scientific Acclaim RSLC Polar Advantage II column (2.1 mm×100 mm, 2.2 μm) using a two-phase gradient elution: A: 0.1% HCOOH in water and B: 0.1% HCOOH in acetonitrile. The amount of CBD in tested samples was obtained based on a calibration curve made of a standard solution of cannabidiol in methanol, purchased from Merck (CRM, 1.0 mg/mL Cerilliant®, where the regression coefficient of the calibration curve was R.sup.2=0.9999 for the limit of detection (LOD) of 1.31 μg/mL and the limit of quantitation (LOQ) of 3.98 μg/mL.

TABLE-US-00012 TABLE 11 Evaluation of the active substance content in aqueous solution at pH = 2.61 before and after simulated pasteurization. CBD content in CBD content in dispersion before dispersion after # Name Dilution pasteurization [%]* pasteurization [%]* 1 W-1 1000x 1 000 98.46 96.22 2 W-1 1000x 1 000 96.28 95.61 3 W-1 1000x 1 000 95.75 95.87 4 W-2 1000x 1 000 97.60 98.74 5 W-2 1000x 1 000 89.77 93.79 6 W-2 1000x 1 000 96.43 98.85 Average results (n = 3) CBD content in CBD content in dispersion before dispersion after No. Name Dilution pasteurization [%]* pasteurization [%]* 70 W-1 1000x 1 000 96.83 ± 1.44 95.90 ± 0.31 74 W-2 1000x 1 000 94.60 ± 4.22 97.13 ± 2.89 *% CBD content based on the theoretical content calculated on the basis of the weight

[0157] The CBD content in the dispersion for systems 70 and 74 exceeds >90% and in both cases amounts to approx. 95%, which confirms that the reported compositions form stable nanoemulsions and meet the requirement to deliver equal doses of the active substance. The attempt to preserve nanoemulsions by pasteurization in simulated conditions for systems 70 and 74 did not affect the CBD content in these systems, and the differences did not exceed 3% of the active substance content.

[0158] Study of the Self-Emulsifying Compositions Stability in Tests of Temperature Transitions (Freeze-Thaw Test)

[0159] Commercial products at the distribution stage are exposed to many external factors. One of them is the effect of temperature, especially during transport. Therefore, it is imperative that the product is able to withstand a certain range of temperature fluctuations during transport. The freeze-thaw cycle test is part of the stability study to determine whether the composition parameters remain stable under various conditions. This type of testing is particularly recommended for liquid products. Phase separation can occur in such products, which can have a negative impact on their performance and quality parameters. The freeze-thaw resistance test involves exposing the product to sub-zero temperature (≤−10° C.) for 24 hours, and then incubating it for 24 hours at room temperature. The sample is then placed at a higher temperature (approximately 45° C.) for 24 hours and then again at room temperature for 24 hours. The sample is analyzed for significant changes. One cycle is thus completed. If no significant changes are observed after three cycles of freeze-thaw testing in a row, it can be assured that the product's stability is sufficient for transport. Accordingly, a test of cyclic freeze-thaw, to which food products/raw materials may undergo during transport, was performed on the compositions described below.

[0160] Stability testing of self-emulsifying compositions containing 10% and 20% of cannabinoid, respectively, was performed based on four compositions of non-optimal proportions, in order to demonstrate the versatility of the technology. In addition to the compositions described above, two further formulations were included in the tests described below: Variant 3 and Variant 4 for compositions with 10% and 20% cannabinoid content, respectively. The exact content of the tested compositions is shown in Table 12.

TABLE-US-00013 TABLE 12 Self-emulsifying compositions tested in the freeze-thaw stability studies. Surfactant Lipid 1 Lipid 2 Cannabinoid No. Description Abbreviation T80 MCT MCM CBD 45 Optimal system CBD 10% 46% 22% 22% 10% 78 Variant 1 W-1 30% 30% 30% 10% 80 Variant 3 W-3 50% 20% 20% 10% 47 Optimal system CBD 20% 37.3%.sup.  20% 22.7%.sup.  20% 79 Variant 2 W-2 30% 25% 25% 20% 81 Variant 4 W-4 50% 15% 15% 20%

[0161] The freeze-thaw durability test was carried out on the four compositions described in Table 12, each experiment in triplicate (n=3), where each composition was subjected to three freeze-thaw cycles (1 CYCLE can be described as follows: 24 hours at temp. −21° C., then the samples were transferred for 24 hours to temp. 22° C., further transferred for 24 hours to temp. 40° C., and then for 24 hours to 22° C.). After each cycle, transmittance (T %), size of the nanoparticles (D.sub.H), nanoparticle size distribution (PdI) were determined, as well as quantitative analysis of CBD was performed using ultra high performance liquid chromatography (UHPLC-DAD). Additionally, the samples were visually inspected for change of color, odor, phase separation etc. The samples showed no organoleptic changes.

TABLE-US-00014 TABLE 13 Values of the quality parameters of the tested compositions in the freeze-thaw tests. Transmittance D.sub.H HPLC # Name Cycle Replicate [%] [nm] Pdl [%] 1 Variant 1 0 (start) 1 97.7 76.4 0.142 99.52 2 (Nr 78) 2 97.6 73.6 0.117 98.68 3 3 97.3 75.6 0.121 100.78 4 1.sup.st 1 98 71.1 0.123 97.96 5 cycle 2 97.7 71.4 0.122 98.64 6 3 97.9 71.9 0.133 102.29 7 2.sup.nd 1 98.9 79.2 0.187 97.11 8 cycle 2 98.6 76.6 0.148 97.13 9 3 98.3 72.1 0.133 100.51 10 3.sup.rd 1 96.3 73.7 0.134 97.03 11 cycle 2 98.5 70.1 0.135 97.22 12 3 97.9 71.7 0.125 100.12 13 Variant 2 0 (start) 1 92.1 104.5 0.137 98.75 14 (Nr 79) 2 89.6 112.3 0.141 98.47 15 3 90.6 108.5 0.147 98.73 16 1.sup.st 1 92.2 102.4 0.117 99.53 17 cycle 2 93.8 103.2 0.14 99.69 18 3 90.4 110.7 0.131 94.1 19 2.sup.nd 1 93.7 104.1 0.166 99.18 20 cycle 2 93 116.9 0.225 98 21 3 94.1 104.4 0.158 99.74 22 3.sup.rd 1 94.2 100.8 0.137 99.23 23 cycle 2 91.1 109.7 0.131 98.78 24 3 94.3 101.4 0.113 99.56 25 Variant 3 0 (start) 1 98.4 49.1 0.371 99.65 26 (Nr 80) 2 99.1 37.8 0.26 96.93 27 3 99.7 35.5 0.213 99.12 28 1.sup.st 1 100 32.4 0.145 98.15 29 cycle 2 100.1 32.5 0.156 95.95 30 3 99.8 32.8 0.138 98.28 31 2.sup.nd 1 99 33.6 0.176 99.32 32 cycle 2 98.6 36.5 0.241 98.17 33 3 98.3 34.3 0.208 96.72 34 3.sup.rd 1 99.4 35.8 0.219 99.44 35 cycle 2 99.4 34.7 0.214 95.68 36 3 99.7 37.2 0.282 97.89 37 Variant 4 0 (start) 1 93.5 115.6 0.193 100.21 38 (Nr 81) 2 93 115.1 0.157 98.97 39 3 93.5 111.7 0.152 98.73 40 1.sup.st 1 91.4 122 0.153 100.08 41 cycle 2 93.9 126.2 0.245 100.14 42 3 95 112.9 0.208 99.57 43 2.sup.nd 1 92.5 126.1 0.219 99.34 44 cycle 2 93.2 114.1 0.161 99.41 45 3 94.5 112.3 0.183 97.05 46 3.sup.rd 1 92 118.7 0.178 99.87 47 cycle 2 93.1 117.2 0.185 98.12 48 3 93.8 112.9 0.171 99.06 Average results (n = 12) Repetitions Transmittance D.sub.H HPLC No. Name Cycles per cycle [%] [nm] Pdl [%] 78 Variant 1 3 3 97.0 ± 0.7 73.62 ± 2.8 0.135 ± 0.019 98.9 ± 1.7 79 Variant 2 3 3 92.4 ± 1.7 106.6 ± 5.0 0.145 ± 0.029 98.6 ± 1.5 80 Variant 3 3 3 99.3 ± 0.6  36.0 ± 4.5 0.219 ± .066  97.9 ± 1.4 81 Variant 4 3 3 93.3 ± 1.0 117.1 ± 5.2 0.184 ± .029  99.2 ± 0.9 UHPLC -% amount of CBD in relation to the nominal value (initial - resulting from the sample weight)

[0162] Stability Testing of Self-Emulsifying Compositions in Commercially Available Beverages

[0163] Compatibility tests of the developed invention with commercially available beverages were performed based on variant 3 (No. 80). This system demonstrated very high transparency and a short dispersion time. The experiment aimed to determine if a given system could be applied to different categories of beverages. For this purpose, 12 commercial beverages supplemented with a self-emulsifying composition that delivered a dose of 10 mg of CBD per the total volume of the unit drink were tested Various drinks were tested in the experiment: water, flavored drinks, juice beverages, fruit drinks, tonic drinks, lemonade, tea beverage, carbonated and non-carbonated drinks, transparent, colored and lightproof, with and without vitamins added, clarified and unclarified, pasteurized and unpasteurized, sweetened and unsweetened, and even beer up to alcohol content of <5%. Water was used as a comparison and the simulated system was an aqueous solution with the addition of 0.8% by weight of citric acid and 0.08% of sodium benzoate.

[0164] Table 14 shows the values of D.sub.H and PdI only in beverages after adding the self-emulsifying composition, because before the addition no nanoparticles were found that would allow measurement by the DLS method, with the exception of drink sample No. 7 (Lipton drink—tea beverage, where D.sub.H and PdI were 154.7 nm and 0.319, respectively).

TABLE-US-00015 TABLE 14 Dispersion tests of the self-emulsifying composition in commercial beverages. Trans. Trans. D.sub.H Commercial V Illustrative pH pH before.sup.a after.sup.b after.sup.b Pdl # name [mL] description before.sup.a after.sup.b [%] [%] [nm] after.sup.b 1 Water 500 control 6.00 5.67 100.0 99.2 37.5 ± 5.3 0.261 2 Aqueous 500 simulated 2.81 2.83 99.5 99.1 43.3 ± 2.3 0.312 solution system 3 Schweppes 900 tonic 2.88 2.97 100.0 98.9 125.1 ± 89.1 0.361 drink 4 Cappy 1000 juice 3.82 3.76 97.2 96.9 UN 0.256 beverage 5 Sprite 500 carbonated 3.01 3.05 100.0 99.4 45.3 ± 1.4 0.379 drink 6 RedBull 250 energy 3.88 3.87 95.2 94.3 66.1 ± 7.0 0.323 drink 7 Lipton 330 tea 3.74 3.73 57.2 52.7 82.6 ± 2.8 0.246 beverage 8 Heineken 500 beer 4.79 4.85 97.1 93.2 163.1 ± 8.4  0.267 9 Coca-Cola 1000 carbonated 3.05 3.06 37.8 37.8 UN 0.508 drink 10 Frugo 250 fruit 3.42 3.42 41.8 40.2 UN 0.988 drink 11 OnLemon 330 lemonade 3.98 3.94 24.6 15.5 UN 0.386 12 Cisowianka 700 sparkling 7.43 7.67 98.9 100.0 46.7 ± 1.4 0.334 water V—volume of the drink, unit packaging .sup.athe original commercial beverage before adding the self-emulsifying composition No. 80 .sup.bthe original commercial drink after adding the self-emulsifying composition No. 80, which provides a dose of 10 mg of CBD for the entire unit packaging UN—no possibility of measuring/recording the results due to the physicochemical characteristics of the sample

[0165] The results show that the composition No. 80 (variant 3) provides for nanoparticle formation in commercially available beverages with a particle size within the range of D.sub.H=37.5÷163.1 nm. Measurement of nanoparticle size was not possible in all cases due to the high turbidity of some beverages. In the case of alcoholic beverages, the presence of alcohol has a noticeable effect on the increase in diameter of nanoparticle in the dispersion system, which is visible in the example of Heineken beer. Nevertheless, the particle size met the acceptance criterion and was below 200 nm. In all cases of the tested beverages, no significant change in pH (above 0.5 on the pH scale) upon addition of the appropriate dose of composition 80 was observed.

[0166] Stability Tests

[0167] Storage tests were performed to determine the long-term stability of the self-emulsifying compositions and the suitability for consumption. In order to determine the longest shelf life and stability in the shortest possible test time, accelerated shelf-life tests (ASLT) were carried out. This method utilizes a kinetic model of chemical reactions that includes all factors that may affect their rate, where in fact the most common way to accelerate the reactions is to place the product at a constant, elevated temperature.

[0168] As part of the stability tests, 4 variants of the composition were analyzed (Nos. 78÷81). Each test was performed in triplicate (n=3). The stability tests over time were performed at 4° C., room temperature (21±2° C.) and at 45° C. (accelerated tests). Compositions stored for 69 days at 45° C. correspond to 12 months of storage at 21° C. After this time, the dispersion time, transmittance (T %), hydrodynamic diameter of nanoparticles (D.sub.H), nanoparticle size distribution (PdI) and CBD content were determined in relation to the nominal value (initial—resulting from the sample weight).

TABLE-US-00016 TABLE 15 Stability of technological parameters of the self-emulsifying compositions during storage. Sample 0 days 69 days CBD Transmittance D.sub.H UHPLC Transmittance D.sub.H UHPLC # Temperature Variant Replicate concentration [%] [nm] Pdl [%] [%] [nm] Pdl [%] 1 4° C. 1 1 10.12% 97.7 76.4 0.142 99.52 97.8 75.9 0.153 97.27 2 No. 78 2 10.11% 97.6 73.6 0.117 98.68 98.1 71.2 0.111 100.53 3 3 9.84% 97.3 75.6 0.121 100.78 97.6 74.9 0.123 99.59 4 2 1 19.93% 92.1 104.5 0.137 98.75 90.4 108.4 0.132 103.66 5 No. 79 2 19.97% 89.6 112.3 0.141 98.47 91.4 109.1 0.148 100.63 6 3 20.02% 90.6 108.5 0.147 98.73 90.2 111.6 0.169 100.4 7 3 1 9.95% 98.4 49.1 0.371 99.65 100 40.3 0.273 98.15 8 No. 80 2 9.87% 99.1 37.8 0.26 96.93 99.1 36.1 0.217 98.47 9 3 10.10% 99.7 35.5 0.213 99.12 99 50.1 0.317 99.54 10 4 1 20.12% 93.5 115.6 0.193 100.21 88.7 131.4 0.168 98.46 11 No. 81 2 19.98% 93 115.1 0.157 98.97 90.4 121.8 0.154 100.18 12 3 19.88% 93.5 111.7 0.152 98.73 92.4 117 0.195 99.13 13 21 ± 2° C. 1 1 10.12% 97.7 76.4 0.142 99.52 98.4 94.2 0.268 101.16 14 No. 78 2 10.11% 97.6 73.6 0.117 98.68 97.9 72.3 0.119 98.73 15 3 9.84% 97.3 75.6 0.121 100.78 98.1 74.3 0.138 102.02 16 2 1 19.93% 92.1 104.5 0.137 98.75 92.8 106.9 0.125 97.68 17 No. 79 2 19.97% 89.6 112.3 0.141 98.47 92 111.1 0.179 99.02 18 3 20.02% 90.6 108.5 0.147 98.73 91.7 108.1 0.155 98.41 19 3 1 9.95% 98.4 49.1 0.371 99.65 99.6 45.5 0.34 101.53 20 No. 80 2 9.87% 99.1 37.8 0.26 96.93 99.5 48.5 0.353 99.65 21 3 10.10% 99.7 35.5 0.213 99.12 99.2 41.5 0.307 96.66 22 4 1 20.12% 93.5 115.6 0.193 100.21 92.5 143.7 0.229 97.47 23 No. 81 2 19.98% 93 115.1 0.157 98.97 93.4 123.2 0.164 100.25 24 3 19.88% 93.5 111.7 0.152 98.73 93.2 126.4 0.243 100.57 25 45° C. 1 1 10.12% 97.7 76.4 0.142 99.52 96.3 73.7 0.134 98.03 26 No. 78 2 10.11% 97.6 73.6 0.117 98.68 98.5 70.1 0.135 98.33 27 3 9.84% 97.3 75.6 0.121 100.78 97.9 71.7 0.125 100.64 28 2 1 19.93% 92.1 104.5 0.137 98.75 94.2 100.8 0.137 99.46 29 No. 79 2 19.97% 89.6 112.3 0.141 98.47 91.1 109.7 0.131 99.1 30 3 20.02% 90.6 108.5 0.147 98.73 94.3 101.4 0.113 99.34 31 3 1 9.95% 98.4 49.1 0.371 99.65 99.4 35.8 0.36 96.06 32 No. 80 2 9.87% 99.1 37.8 0.26 96.93 99.4 34.7 0.214 96.32 33 3 10.10% 99.7 35.5 0.213 99.12 99.7 37.2 0.282 97.3 34 4 1 20.12% 93.5 115.6 0.193 100.21 92 118.7 0.178 99.43 35 No. 81 2 19.98% 93 115.1 0.157 98.97 93.1 117.2 0.185 98.99 36 3 19.88% 93.5 111.7 0.152 98.73 93.8 112.9 0.171 93.7

[0169] Based on Table 15 regarding the stability of self-emulsifying compositions over the studied storage time, along with the accelerated shelf-life tests, the minimum shelf life of the product that maintains the physicochemical, technological and process parameters of the compositions disclosed is 12 months (as confirmed in the studies), where in order to maintain a dose of cannabinoids or relevant cannabinoid extract, it is recommended to use an excess of 5% as the active substance.

[0170] Procedure for the Preparation of Nanoemulsions at the Industrial Scale

[0171] The production of nanoemulsion at the industrial (semi-technical) scale was carried out on a Kates thermostatic mixer with a volume of 5 liters, equipped with a single blade impeller powered by a 0.75 kW Basel S.A Cantoni® Group motor controlled by an inverter.

[0172] The nanoemulsions were prepared by pouring 3500 mL of water into the mixer, to which 1400 mg of the composition containing: T80:MCT:MCM:CBD, 50:20:20:10% by weight was added. The mixture was mixed for 1 min at 17% of engine power for the variant 1 and at 55% of engine power for the variant 2. After this time, the samples were analyzed. In both cases, the temperature during the process was 23.1±0.2° C. as indicated by the temperature sensor of the mixer.

TABLE-US-00017 TABLE 16 Study może lepiej Evaluation of nanoemulsions obtained at the industrial scale. Mixing Transmittance Nr Name power [%] D.sub.H [nm] Pdl 78 Variant 1 17% 99.8 ± 0.1 34.1 ± 0.2 0.175 ± 0.007 79 Variant 2 55% 99.9 ± 0.1 32.4 ± 0.2 0.156 ± 0.024

[0173] The preferred examples of the described compositions are scalable and form nanoemulsions at both laboratory and industrial scale. The results summarized in Table 16 show that the transparency of the solutions measured by transmittance (% T), the nanoparticle hydrodynamic diameter and the polydispersity of the systems obtained at a semi-technical scale meet the above-mentioned requirements. In both cases (78 and 79) the transmittance is close to 100% and the hydrodynamic diameter does not exceed 35 nm. Both systems are also monodisperse, and the PdI value does not exceed 0.180.

[0174] The type of mixing used and the mixing speed have an impact on the quality of the nanoemulsion, but the advantage of the claimed compositions is that desired nanoemulsion parameters can be obtained with minimal mixing.

[0175] The studied compositions containing Tween™ 80 as a surfactant in the amount of 50% by weight also confirmed that the surfactant content did not adversely affect the nanoemulsion formation process itself and did not cause foaming. Foaming is an undesirable effect and is a common technological problem, especially at the industrial scale. The described compositions and method of use thereof do not have this problem even at the highest surfactant concentrations.