Cocktail Vaccine of Recombinant African Swine Fever Virus (ASFV) Antigen and Use Thereof
20230218736 · 2023-07-13
Assignee
Inventors
- Junjun SHAO (Lanzhou, CN)
- Guanglei ZHANG (Lanzhou, CN)
- Wei LIU (Lanzhou, CN)
- Xiaxia LIANG (Lanzhou, CN)
- Huiyun CHANG (Lanzhou, CN)
Cpc classification
C12N2710/12022
CHEMISTRY; METALLURGY
A61K39/295
HUMAN NECESSITIES
International classification
A61K39/295
HUMAN NECESSITIES
Abstract
The present disclosure relates to a cocktail vaccine of a recombinant African swine fever virus (ASFV) antigen and use thereof, and belongs to the field of biotechnology pharmacy. The cocktail vaccine is prepared by combination of recombinant proteins p30-modified p54, p72 epitope-N-segment amino acid sequence of pE248R, and N-terminal amino acid sequence of CD2v-C-terminal amino acid sequence of pEP153R with an adjuvant. The present disclosure provides a method for expressing and purifying the three recombinant proteins, as well as a ratio of each recombinant protein and the adjuvant for preparing the cocktail vaccine.
Claims
1. A cocktail vaccine of a recombinant African swine fever virus (ASFV) antigen, wherein the cocktail vaccine comprises two or more of three recombinant ASFV antigen fusion proteins PM, PPE, and CPE as active ingredients; the PM is formed by fusion of an ASFV p30 and a modified p54 through a Linker, with a general formula of p30-(Linker).sub.3-mp54; the PPE is formed by fusion of four verified epitopes of an ASFV p72 and an N-segment amino acid sequence of pE248R through the Linker, with a general formula of p72 epitope 1-(Linker).sub.3-p72 epitope 2-(Linker).sub.3-p72 epitope 3-(Linker).sub.3-p72 epitope 4-(Linker).sub.3-N-segment amino acid sequence of pE248R; the CPE is formed by fusion of an N-segment amino acid sequence of CD2v and a C-segment amino acid sequence of pEP153R through the Linker, with a general formula of N-segment amino acid sequence of CD2v-(Linker).sub.2-C-segment amino acid sequence of pEP153R; and the Linker has the sequence set forth in SEQ ID NO:7.
2. The cocktail vaccine according to claim 1, wherein the PM has the amino acid sequence set forth in SEQ ID NO: 4; the PPE has the amino acid sequence set forth in SEQ ID NO: 5; and the CPE has the amino acid sequence set forth in SEQ ID NO: 6.
3. The cocktail vaccine according to claim 2, wherein the nucleotide sequence encoding the PM is set forth in SEQ ID NO: 1; the nucleotide sequence encoding the PPE is set forth in SEQ ID NO: 2; and the nucleotide sequence encoding the CPE is set forth in SEQ ID NO: 3.
4. The cocktail vaccine according to claim 1, wherein the cocktail vaccine comprises three recombinant ASFV antigen fusion proteins PM, PPE, and CPE as active ingredients.
5. The cocktail vaccine according to claim 4, further comprising an adjuvant, wherein the cocktail vaccine is prepared by mixing the PM, the PPE, and the CPE with the adjuvant.
6. The cocktail vaccine according to claim 5, wherein the three recombinant ASFV antigen fusion proteins PM, PPE, and CPE are mixed in a mass ratio of 1:1:2 to obtain an antigen mixture; and the antigen mixture is emulsified into the cocktail vaccine with equal quality ISA 206 adjuvant.
7. A method for preparing a drug for treating and preventing ASFV infection, wherein comprises using the cocktail vaccine according to claim 1.
8. The cocktail vaccine according to claim 4, wherein the PM has the amino acid sequence set forth in SEQ ID NO: 4; the PPE has the amino acid sequence set forth in SEQ ID NO: 5; and the CPE has the amino acid sequence set forth in SEQ ID NO: 6.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0021]
[0022]
[0023]
[0024]
[0025]
[0026]
[0027]
DETAILED DESCRIPTION OF THE EMBODIMENTS
[0028] Inventors of the present disclosure found that three recombinant ASFV antigen fusion proteins are obtained by gene fusion and tandem expression, namely p30-modified p54, p72 epitope-N-terminal amino acid sequence of pE248R, and N-terminal amino acid sequence of CD2v-C-terminal amino acid sequence of pEP153R. The three recombinant proteins are combined with an adjuvant in a certain ratio to formulate a cocktail vaccine, and the cocktail vaccine can induce neutralizing antibodies and cellular immune response in pigs after immunizing, suggesting that this vaccine type is a promising ASF subunit vaccine for use. The three recombinant ASFV antigen fusion proteins are as follows:
[0029] (1) a recombinant ASFV antigen fusion protein PM, the recombinant protein is formed by fusion of ASFV p30 and modified p54 (mp54) through a linker peptide (Linker), with a general formula of p30-(Linker).sub.3-mp54;
[0030] (2) a recombinant ASFV antigen fusion protein PPE, the recombinant protein is formed by fusion of four epitopes of ASFV p72 and an N-terminal amino acid sequence of pE248R through the linker peptide (Linker), with a general formula of p72 epitope 1-(Linker).sub.3-p72 epitope 2-(Linker).sub.3-p72 epitope 3-(Linker).sub.3-p72 epitope 4-(Linker).sub.3-N-terminal amino acid sequence of pE248R;
[0031] (3) a recombinant ASFV antigen fusion protein CPE, the recombinant protein is formed by fusion of an N-terminal amino acid sequence of CD2v and a C-terminal amino acid sequence of pEP153R through the linker peptide (Linker), with a general formula of N-terminal amino acid sequence of CD2v-(Linker).sub.2-C-terminal amino acid sequence of pEP153R; and the Linker has a sequence of GGGGS.
[0032] In the present embodiments, the recombinant ASFV antigen fusion proteins PM, PPE, and CPE have amino acid sequences set forth in SEQ ID NO: 4, SEQ ID NO: 5, and SEQ ID NO: 6, respectively.
[0033] In the present embodiments, nucleotide sequences encoding the recombinant ASFV antigen fusion proteins PM, PPE, and CPE are set forth in SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3, respectively.
[0034] A method for expression, purification, and identification of the recombinant proteins includes:
[0035] (1) vector construction: constructing a recombinant expression vector, where the expression vector is formed by linking the nucleotide sequence encoding the recombinant protein in a modifiable manner by a backbone plasmid; in some embodiments, the backbone plasmid is pET-30a (+);
[0036] (2) transformation and screening of positive clones: transforming the recombinant expression vector in step (1) into competent cells of host bacteria, and obtaining recombinant bacteria capable of expressing a target protein after induction and SDS-PAGE identification;
[0037] (3) induced expression: transferring positive recombinant bacteria obtained in step (2) to grow to a certain concentration, and adding IPTG to induce expression of the recombinant protein;
[0038] (4) protein purification: collecting bacterial cells in step (3), and conducting ultrasonication, dissolution of inclusion bodies, purification by Ni-NTA affinity chromatography, and dialysis renaturation to obtain a target protein; and
[0039] (5) identification of the recombinant protein: identifying the target protein obtained in step (4) by SDS-PAGE and Western blotting.
[0040] The present disclosure further provides a method for preparing a cocktail vaccine of recombinant ASFV antigens with the recombinant proteins, including the following steps:
[0041] determination of a protein concentration: determining a concentration of a purified recombinant protein by a Bradford method;
[0042] preparation of an antigen mixture: diluting recombinant proteins to the same concentration and mixing in a certain ratio; and
[0043] emulsification of a vaccine: conducting emulsification on an antigen mixture obtained in step (2) with a MONTANIDE™ ISA 206 VG adjuvant (50 g: 50 g) to prepare a vaccine.
[0044] To better understand the present disclosure, the content of the present disclosure is further described below in details in conjunction with the examples, but the claimed content of the present disclosure is not limited to the following examples.
[0045] I. Experimental Materials
[0046] 1.1. Cells and Viruses
[0047] BL21(DE3) competent cells were purchased from Sangon (Shanghai); porcine alveolar macrophages were isolated from lungs of healthy pig and stored in liquid nitrogen by the research group; and ASFV (CN/SC/2019) strains were isolated and preserved by the Lanzhou Veterinary Research Institute of the Chinese Academy of Agricultural Sciences (National ASF Regional Laboratory).
[0048] 1.2. Experimental Animals
[0049] 6-week-old healthy female piglets were purchased from a farm in Kangle County, Gansu Province.
[0050] II. Experimental methods and results
[0051] 2.1. Construction of a Recombinant Plasmid
[0052] (1) According to an existing ASFV, ASFV-SY18 (accession number: MH766894.1) in GenBank, the amino acid sequences of p30 and p54 were selected, where a transmembrane region His30-Phe52 of p54 replaced a linking peptide (GGGGS).sub.3 to obtain mP54, and the mP54 was ligated with a C-terminal amino acid sequence of p30 through the linking (GGGGS).sub.3, to obtain an amino acid sequence of a recombinant fusion protein PM. (2) According to the 4 epitopes located in ASFV p72 (epitope IDs: 141844, 141941, 141989, and 142069) that have been verified in the Immune Epitope Database (IEDB) database, the epitopes were ligated in series through the (GGGGS).sub.3 flexible Linker, and the ligated p72 epitope was ligated with an N-terminal amino acid sequence (Metl-Lysl98) of pE248R (protein ID: AYW34102.1) of ASFV, ASFV-SY18, through the (GGGGS).sub.3 flexible Linker in series, to obtain an amino acid sequence of a recombinant fusion protein PPE. (3) The N-terminal amino acid sequence (Asp17-Tyr206) of ASFV-SY18 CD2v (Protein ID: AYW34030.1) and the C-terminal amino acid sequence (Asn49-Lys158) of pEP153R (Protein ID: AYW34029.1) were ligated in series by a (GGGGS).sub.2 flexible Linker, to obtain an amino acid sequence of a recombinant fusion protein CPE. The amino acid sequences of the above three recombinant fusion proteins were converted into corresponding nucleotide sequences according to the codon preference of E. coli, and specific enzyme cleavage sites BamH I and Xho I were introduced at 5′- and 3′- ends of each nucleotide sequence; the obtained nucleotide sequences were entrusted to Nanjing GenScript Biotechnology Co., Ltd. to synthesize and clone into a pET-30a(+) expression vector, to obtain recombinant expression plasmids pET-30a(+)-PM, pET-30a(+)-PPE, and pET-30a(+)-CPE. The results of gene sequencing showed that the sequences between BamH I and Xho I on the three recombinant plasmids had 100% homology with the designed target genes, and the reading frame was completely correct.
[0053] 2.2. Expression and Purification of Recombinant Proteins
[0054] The three recombinant expression plasmids were transformed into E. coli BL21 (DE3) competent cells separately, and positive clones were selected and inoculated into 5 mL of LB (kan.sup.+), incubated at 37° C. for 8 h at 220 rpm, and then inoculated into 1 L of LB (kan.sup.+) at 1:100, cultured at 37° C. at 220 rpm, when the OD.sub.600 of the bacterial solution reached 0.4 to 0.6, a final concentration of 1 mM of IPTG was added, and continued to culture for 4 h at 37° C. at 220 rpm. Bacteria were collected by centrifugation at 7,000 rpm for 6 min, resuspended in 50 mL of a binding buffer (300 mM NaCl, 20 mM NaH.sub.2PO.sub.4, and 5 mM imidazole; at pH 8.0), sonicated for 40 min (in ice bath), and centrifuged at 10,000 rpm for 25 min to collect precipitation (inclusion bodies). The inclusion bodies were dissolved with a binding buffer containing 8 M urea, centrifuged at 10,000 rpm for 25 min, a supernatant was collected, transferred to a Ni-NTA affinity chromatography column, and bound at 4° C. for 1.5 h. The impure proteins were washed with 10 column volumes of washing buffer (8 M urea, 300 mM NaCl, 20 mM NaH.sub.2PO.sub.4, and 20 mM imidazole; at pH 8.0), and a target recombinant protein was eluted with an appropriate amount of elution buffer (8 M urea, 300 mM NaCl, 20 mM NaH.sub.2PO.sub.4, and 500 mM imidazole; at pH 8.0). The obtained recombinant protein was dialyzed and renatured by 8 M, 6 M, 4 M, 2 M and 0 M of dialysate (20 mM NaH.sub.2PO.sub.4, 300 mM NaCl, 2 mM β-mercaptoethanol, 0.4% arginine, and 10% glycerol; at pH 7.5) in sequence, each gradient was dialyzed for 8 h, and a small amount of renatured recombinant protein was taken for SDS-PAGE. The results showed that the renatured recombinant proteins PM, PPE, and CPE were 56 kDa, 46 kDa, and 32 kDa, respectively, as shown in
[0055] 2.3. Western Blotting Identification of Recombinant Protein
[0056] 80 μL of the three recombinant proteins were mixed with a 5× loading buffer and boiled for 10 min separately; after loading, SDS-PAGE was conducted (80 V for 30 min, 120 V for 1 h and 10 min), and then electrotransferred to a polyvinylidene fluoride (PVDF) membrane, and blocked in a 1× PBST solution with 5% nonfat dry milk for 2 h at room temperature. Primary antibody incubation: the blocked PVDF membrane was incubated with a mouse anti-6×His monoclonal antibody (with 1:5000-fold dilution) and anti-ASFV positive serum (with 1:300-fold dilution) overnight at 4° C., and washed with 1× PBST for 6 times. Secondary antibody incubation: peroxidase-labeled goat anti-mouse IgG (with 1:5000-fold dilution) and peroxidase-labeled goat anti-pig IgG (with 1:5000-fold dilution) were added to the corresponding PVDF membranes, incubated at room temperature for 1 h, and washed with 1× PBST for 6 times. Color development: an ECL luminescent solution was prepared and spread evenly on the PVDF membranes; and image acquisition was conducted with a multi-function imager.
[0057] Results and analysis: the results of recombinant protein identification by Western blotting were shown in
[0058] 2.4. Vaccine Preparation and Immunization Scheme
[0059] The concentration of three recombinant proteins was separately determined by a Bradford method: 600 μg of the PM, 600 μg of the PPE, and 1,200 μg of the CPE were mixed and diluted to a total volume of 4 mL, and then mixed with a MONTANIDE™ ISA 206 VG adjuvant (50 g: 50 g) for emulsification to prepare a water/oil/water vaccine (W/O/W). Seven 6-week-old female piglets were randomly divided into 2 groups (4 in an experimental group and 3 in a control group); the prepared vaccine was injected intramuscularly, according to immunization groups and immunization dosages in the following table, with primary immunization for 1 time and booster immunization for 1 time after 21 d.
TABLE-US-00001 Immune Injection Group component/head volume mL/head Experimental 150 μg PM + 150 μg 2 group 1 PPE + 300 μg CPE Control group PBS 2
[0060] Results and analysis: a standard curve was established according to the OD value of the different concentrations of protein standards at 592 nm. A linear equation, y=0.00049964x+0.28323, R.sup.2=0.993, was obtained according to the standard curve. The concentrations of the of the recombinant proteins PM, PPE and CPE were obtained according to the linear equation that were 677.1 μg/mL, 896.2 μg/mL, and 494.1 μg/mL, respectively.
[0061] 2.5. Evaluation of Immune Effect
[0062] 2.5.1. Antigen-Specific IgG Detection
[0063] Blood was collected from all experimental pigs before and 14, 21, 35 and 42 d after immunization, and the serum was separated by centrifugation at 4,000 rpm for 10 min. Detection of specific antibodies of immune sera was conducted by laboratory established indirect ELISA based on the recombinant proteins including p30, p54, p72, pE248R, CD2v, and pEP153R, respectively.
[0064] Detection procedure: the 96-well microtiter plate were separately coating with the purified recombinant p30 (0.125 μg/mL), p54 (0.5 μg/mL), p72 (1 μg/mL), pE248R (1 μg/mL), CD2v (2 μg/mL), and pEP153R (2 μg/mL) to overnight at 4° C., 100 μL/well; coating solution was discarded, blocking solution (5% nonfat dry milk, 200 μL/well) was added and incubated at 37° C. for 2 h; the blocking solution was discarded, serum samples diluted at 1:100 were added, 100 L/well, and then incubated at 37° C. for 1 h; the liquid was discarded and the plate was washed 5 times with 1×PBST; 1:10000-diluted horseradish peroxidase (HRP)-labeled goat anti-pig IgG was added, 100 μL/well, and then incubated at 37° C. for 1 h; the liquid was discarded and the plate was washed 5 times with 1×PBST; then TMB substrate was added, 100 μL/well, and reacted at room temperature for 10 min in the dark; stop solution (2 M H.sub.2SO.sub.4) was added, 100 VL/well, and the OD value was measured at 450 nm.
[0065] As shown in
[0066] 2.5.2. Lymphocyte Proliferation Assay
[0067] 42 d after Immunization, 5 mL of Anticoagulant Blood was Collected from Each Immunized Pig and diluted with an equal volume of sterile PBS, and then the anticoagulant blood was slowly and lightly added to a 50 mL centrifuge tube containing 10 mL of lymphocyte separation medium (Ficoll-paque, 1.077 g/ml); centrifugation was conducted at 800×g for 30 min (at room temperature) in a horizontal rotor centrifuge; the buffy coat (peripheral blood mononuclear lymphocyte cells, PBMCs) was transferred to a new centrifuge tube, cells were resuspended in 20 mL of sterile PBS, centrifuged at 250×g for 10 min, and the supernatant was discarded, the cell pellet was repeated once according to mention-above; the cells were resuspended in 0.5 mL PBS, and mixed evenly and quickly with 0.5 mL of 2.5 μM of CFSE working solution; the cell suspension was placed in a water bath at 37° C. for 10 min, and shaken once every 2 min; added with 5 mL of RPMI-1640, centrifuged at 1,500 rpm for 5 min to discard the supernatant, and the step was repeated once; the PBMCs was resuspended in RPMI-1640 containing 10% FBS, adjusted to 1×10.sup.6 cells/mL, and plated into a 24-well plate, 1 mL/well. The inactivated ASFV (virus titer is 10.sup.5 HAD.sub.50) was added to each well of the experimental group, and a normal cell group, a non-stimulatory substance group and ConA (5 μg/mL) stimulation group were set up, as blank control, negative control and positive control, respectively, and all group were cultured at 37° C., 5% CO.sub.2 for 72 h; the cells were collected by centrifugation at 1,500 rpm for 5 min, washed twice with PBS and resuspended in 100 μL PBS; fluorescence intensity in the PBMCs was measured by flow cytometry. The cell populations with lower fluorescence intensity were circled based on the negative control, and their percentages in total lymphocytes were calculated.
[0068] As shown in
[0069] 2.5.3. Detection of Antigen-Specific CD8+T Lymphocytes
[0070] PBMCs were separated as described in 2.6, adjusted to a cell density of 1×10.sup.6 cells/mL, and inoculated in a 24-well plate, 1 mL per well. Cells from each well of the experimental group was added with inactivated ASFV (virus tier was 10.sup.5 HAD.sub.50), where wells without stimulant were used as negative control, and wells added with 25 μg/mL phorbol ester and 1 μg/mL ionomycin were used as positive control, and cultured at 37° C., 5% CO.sub.2 for 40 h, and then added with monensin, 1.7 μg/mL/well, and continued to incubate at 37° C., 5% CO.sub.2 for 8 h. All cells in each well were collected, washed twice with PBS, resuspended in 100 μL of PBS. And then added with PerCP-Cy5.5-labeled anti-CD3 monoclonal antibody and PE-labeled anti-CD8 monoclonal antibody (1μ1 each antibody), and stained at 4° C. for 30 min. The cells was washed twice with PBS by centrifugation at 1,500 rpm for 5 min, and then resuspended with 500 μL of a fixative according to instructions of the Fixaion and Permeabiliation solution and kept for 20 min at room temperature in the dark. The cells were washed once with 1 mL of 1×Perm/Wash, and the cells were collected by centrifugation at 1,500 rpm for 5 min. The cell pellets were resuspended with 1.5 mL of the 1×Perm/Wash and incubated for 5 min at room temperature in the dark. The cells were collected by centrifugation at 1,500 rpm for 5 min, and resuspended in 100 μL of the 1×Perm/Wash. The cells were stained with AF700-labeled anti-IL-2, Pecy7-labeled anti-TNF-α and AF647-labeled anti-IFN-γ monoclonal antibodies at 4° C. in the dark for 30 min. The cells were washed twice with the 1×Perm/Wash by centrifugation, and resuspended in 100 μL of PBS containing 2% FBS for flow cytometry analysis to determine the percentages of the CD8+T cells positive for IFN-γ, IL-2 and TNF-α in the T lymphocytes.
[0071] As shown in
[0072] 2.5.4. Indirect Immunofluorescence Assay
[0073] On 42 d after immunization, blood was collected from each immunized pig to prepare serum; the immune serum was diluted at 1:5 and then inactivated at 56° C. for 30 min. The serum was mixed with an equal volume of ASFV (CN/SC/2019) (MOI=0.01), and incubated at 37° C. overnight. The monolayer of PAM cells was inoculated with the mixture of the immune serum and virus (24-well plate), 200 μL per well, at 37° C., 5% CO.sub.2 for 1 h, and shaken gently every 10 min, The mixture of serum and virus was discarded and washed 3 times with PBS. Each well was added with 0.5 mL of RPMI-1640 containing 5% FBS and incubated for 48 h at 37° C., 5% CO.sub.2. The cells were washed 3 times with PBS, added with 4% paraformaldehyde (0.5 mL/well), and incubated at 4° C. for 1 h; the paraformaldehyde was discarded and each well was added with 0.5 mL of 0.25% triton X-100 and allowed to stand for 10 min at room temperature; the cells were washed with PBS 3 times, 3 min in each time, and mixed on a micro shaker; 1 mL of 5% BSA was added to each well to block for 60 min; the blocking solution was discarded, each well was added with 0.5 mL of a mouse anti-p30 monoclonal antibody diluted at 1:2000, and incubated at 37° C. for 60 min; the liquid in the well was discarded and washed 3 times with PBS, each well was added with 0.5 mL of 1:500-diluted TRITC-labeled goat anti-mouse IgG in the dark, and incubated at 37° C. for 60 min; the liquid in the wells was discarded and washed 3 times with PBS, each well was added with two drops of DAPI and supplemented with 0.5 mL PBS, allowed to stand for 5 min at room temperature, and was washed 3 times with 1 mL PBS; and at last, each well was added with 0.5 mL of PBS and then observed and photographed with a Leica DM16000B inverted fluorescence microscope.
[0074] As shown in
[0075] 2.5.5. Virus Neutralization Experiment
[0076] The serum from 0 and 42 d after the primary immunization was diluted at 1:5, filtered and sterilized with a 0.22 μm syringe filter, and then inactivated at 56° C. for 30 min; the serum was mixed with an equal volume of ASFV (MOI=0.01) and incubated overnight at 37° C. A serum/virus mixture was inoculated with a monolayer of porcine alveolar macrophages (PAM) (24-well plate), at 200 μL per well, incubated at 37° C., 5% CO.sub.2 for 1 h. The mixture of serum and virus was discarded and washed 3 times with PBS; each well was added with 500 μL of RPMI-1640 containing 5% FBS, and incubated at 37° C., 5% CO.sub.2 for 48 h; the cells were collected, and ASFV genomes of each experimental group were extracted by a DNA genome extraction kit; a qPCR kit was used to amplify the ASFV gene, the number of ASFV genome copies in the sample was calculated, and a virus neutralization rate was indirectly calculated: virus neutralization rate (%)=100-100*ASFV copy number after incubation with immune serum/ASFV copy number after incubation with pre-immune serum.
[0077] As shown in
[0078] With the pre-immune serum as a control, the inhibition rate of the immune serum from the vaccine group against ASFV infection was 81.6% (
[0079] It should be noted that the above embodiments should be construed as illustrative rather than limiting the claimed scope of the present disclosure. The claimed scope of the present disclosure is subject to the claims. For those skilled in the art, without departing from the spirit and scope of the present disclosure, some non-essential improvements and adjustments made still belong to the claimed scope of the present disclosure. [0080] Sequence Listing Information: [0081] DTD Version: V1_3 [0082] File Name: HLP20220602625.xml [0083] Software Name: WIPO Sequence [0084] Software Version: 2.0.0 [0085] Production Date: 2022 Aug. 19 [0086] General Information: [0087] Current application/Applicant file reference: HLP20220602625 [0088] Earliest priority application/IP Office: CN [0089] Earliest priority application/Application number: 202210024618.6 [0090] Earliest priority application/Filing date: 2022-01-11 [0091] Applicant name: Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences [0092] Applicant name/Language: en [0093] Invention title: COCKTAIL VACCINE OF RECOMBINANT AFRICAN SWINE FEVER VIRUS (ASFV) ANTIGEN AND USE THEREOF (en) [0094] Sequence Total Quantity: 7 [0095] Sequences: [0096] Sequence Number (ID): 1 [0097] Length: 1176 [0098] Molecule Type: DNA [0099] Features Location/Qualifiers: [0100] source, 1 . . . 1176 [0101] >mol_type, other DNA [0102] >note, Nucleotide sequence encoding the PM [0103] >organism, synthetic construct [0104] Residues:
TABLE-US-00002 atggacttca ttctgaacat cagcatgaag atggaagtta tcttcaagac cgacctgcgt 60 agcagcagcc aagtggtttt ccacgcgggt agcctgtaca actggtttag cgttgagatc 120 attaacagcg gccgtattgt gaccaccgcg atcaaaaccc tgctgagcac cgtgaagtat 180 gacattgtta aaagcgcgcg tatctacgcg ggtcagggct ataccgaaca ccaggcgcaa 240 gaggaatgga acatgattct gcacgtgctg ttcgaggaag agaccgagag cagcgcgagc 300 agcgaaaaca tccacgagaa gaacgataac gaaaccaacg agtgcaccag cagcttcgaa 360 accctgtttg aacaagagcc gagcagcgag gttccgaagg acagcaaact gtacatgctg 420 gcgcagaaaa ccgtgcaaca cattgaacag tatggcaagg cgccggattt caacaaagtt 480 atccgtgcgc acaactttat tcagaccatc tacggcaccc cgctgaagga agaggaaaaa 540 gaggtggttc gtctgatggt gatcaagctg ctgaagaaaa agggtggcgg tggcagcggt 600 ggcggtggca gcggtggcgg tggcagcatg gatagcgaat tcatggatag tgaatttttc 660 cagccggtgt atccgcgcca ttatggcgaa tgtctgagtc cggtgaccac cccgagcttt 720 tttagcaccg gtggcggtgg cagcggtggc ggtggcagcg gtggcggtgg cagcagcagc 780 cgcaaaaaaa aagcagccgc aattgaagaa gaagatattc agtttatcaa cccgtatcag 840 gatcagcagt gggttgaagt gaccccgcag ccgggtacca gtaaaccggc aggtgcaacc 900 accgcaagtg tgggtaaacc ggtgaccggc cgtccggcaa ccaatcgtcc ggccaccaat 960 aaaccggtta ccgataatcc ggttaccgac cgcctggtga tggccaccgg tggtccggca 1020 gcagcaccgg cagcagctag tgcaccggca catccggcag aaccgtatac caccgtgacc 1080 acccagaata ccgccagtca gaccatgagc gccattgaaa atctgcgtca gcgtaatacc 1140 tatacccata aagatctgga aaatagtctg ctcgag 1176 [0105] Sequence Number (ID): 2 [0106] Length: 1206 [0107] Molecule Type: DNA [0108] Features Location/Qualifiers: [0109] source, 1 . . . 1206 [0110] >mol_type, other DNA [0111] >note, Nucleotide sequence encoding the PPE [0112] >organism, synthetic construct [0113] Residues:
TABLE-US-00003 atgcagaaag acctggttaa cgaattcccg ggtctgttcg ttcgtcagtc tcgtttcatc 60 gcgggtcgtc cgtctcgtcg taacatccgt ttcaaaccgg gtggtggtgg ttctggtggt 120 ggtggttctg gtggtggtgg ttctgcgtgc tcttctatct ctgacatctc tccggttacc 180 tacccgatca ccctgccgat catcaaaaac atctctgtta ccgcgcacgg tatcaacctg 240 atcgacaaag gtggtggtgg ttctggtggt ggtggttctg gtggtggtgg ttcttactgc 300 gaatacccgg gtgaacgtct gtacgaaaac gttcgtttcg acgttaacgg taactctctg 360 gacgaatact cttctgacgt taccaccctg ccgggtctga aaccgcgtga agaataccag 420 ccgtctggtg gtggtggttc tggtggtggt ggttctggtg gtggtggttc tctgtgcaac 480 atccacgacc tgcacaaacc gcaccagtct aaaccgatcc tgaccgacga aaacgacacc 540 cagcgtacct gctctcacac caacccgggt ggtggtggtt ctggtggtgg tggttctggt 600 ggtggtggtt ctatgggtgg ttctacctct aaaaactctt tcaaaaacac caccaacatc 660 atctctaact ctatcttcaa ccagatgcag tcttgcatct ctatgctgga cggtaaaaac 720 tacatcggtg ttttcggtga cggtaacatc ctgaaccacg ttttccagga cctgaacctg 780 tctctgaaca cctcttgcgt tcagaaacac gttaacgaag aaaacttcat caccaacctg 840 tctaaccaga tcacccagaa cctgaaagac caggaagttg cgctgaccca gtggatggac 900 gcgggtaccc acgaccagaa aaccgacatc gaagaaaaca tcaaagttaa cctgaccacc 960 accctgatcc agaactgcgt ttcttctctg tctggtatga acgttctggt tgttaaaggt 1020 aacggtaaca tcgttgaaaa cgcgacccag aaacagtctc agcagatcat ctctaactgc 1080 ctgcagggtt ctaaacaggc gatcgacacc accaccggta tcaccaacac cgttaaccag 1140 tactctcact acacctctaa aaacttcttc gacttcatcg cggacgcgat ctctgcggtt 1200 ttcaaa 1206 [0114] Sequence Number (ID): 3 [0115] Length: 945 [0116] Molecule Type: DNA [0117] Features Location/Qualifiers: [0118] source, 1 . . . 945 [0119] >mol_type, other DNA [0120] >note, The nucleotide sequence encoding the CPE [0121] >organism, synthetic construct [0122] Residues:
TABLE-US-00004 atggactact gggtttcttt caacaaaacc atcatcctgg actctaacat caccaacgac 60 aacaacgaca tcaacggtgt ttcttggaac ttcttcaaca actctttcaa caccctggcg 120 acctgcggta aagcgggtaa cttctgcgaa tgctctaact actctacctc tatctacaac 180 atcaccaaca actgctctct gaccatcttc ccgcacaacg acgttttcga caccacctac 240 caggttgttt ggaaccagat catcaactac accatcaaac tgctgacccc ggcgaccccg 300 ccgaacatca cctacaactg caccaacttc ctgatcacct gcaaaaaaaa caacggcacc 360 aacaccaaca tctacctgaa catcaacgac accttcgtta aatacaccaa cgaatctatc 420 ctggaataca actggaacaa ctctaacatc aacaacttca ccgcgacctg catcatcaac 480 aacaccatct ctacctctaa cgaaaccacc ctgatcaact gcacctacct gaccctgtct 540 tctaactact tctacacctt cttcaaactg tacgagctcg gtggtggtgg ttctggtggt 600 ggtggttctc atatgaacaa accgatctgc taccagaacg acgacaaaat cttctactgc 660 ccgaaagact gggttggtta caacaacgtt tgctactact tcggtaacga agaaaaaaac 720 tacaacaacg cgtctaacta ctgcaaacag ctgaactcta ccctgaccaa caacaacacc 780 atcctggtta acctgaccaa aaccctgaac ctgaccaaaa cctacaacca cgaatctaac 840 tactgggtta actactctct gatcaaaaac gaatctgttc tgctgcgtga ctctggttac 900 tacaaaaaac agaaacacgt ttctctgctg tacatctgct ctaaa 945 [0123] Sequence Number (ID): 4 [0124] Length: 392 [0125] Molecule Type: AA [0126] Features Location/Qualifiers: [0127] source, 1 . . . 392 [0128] >mol_type, protein [0129] >note, Recombinant ASFV antigen fusion proteins PM [0130] >organism, synthetic construct [0131] Residues:
TABLE-US-00005 MDFILNISMK MEVIFKTDLR SSSQVVFHAG SLYNWFSVEI INSGRIVTTA IKTLLSTVKY 60 DIVKSARIYA GQGYTEHQAQ EEWNMILHVL FEEETESSAS SENIHEKNDN ETNECTSSFE 120 TLFEQEPSSE VPKDSKLYML AQKTVQHIEQ YGKAPDFNKV IRAHNFIQTI YGTPLKEEEK 180 EVVRLMVIKL LKKKGGGGSG GGGSGGGGSM DSEFMDSEFF QPVYPRHYGE CLSPVTTPSF 240 FSTGGGGSGG GGSGGGGSSS RKKKAAAIEE EDIQFINPYQ DQQWVEVTPQ PGTSKPAGAT 300 TASVGKPVTG RPATNRPATN KPVTDNPVTD RLVMATGGPA AAPAAASAPA HPAEPYTTVT 360 TQNTASQTMS AIENLRQRNT YTHKDLENSL LE 392 [0132] Sequence Number (ID): 5 [0133] Length: 402 [0134] Molecule Type: AA [0135] Features Location/Qualifiers: [0136] source, 1 . . . 402 [0137] >mol_type, protein [0138] >note, Recombinant ASFV antigen fusion proteins PPE [0139] >organism, synthetic construct [0140] Residues:
TABLE-US-00006 MQKDLVNEFP GLFVRQSRFI AGRPSRRNIR FKPGGGGSGG GGSGGGGSAC SSISDISPVT 60 YPITLPIIKN ISVTAHGINL IDKGGGGSGG GGSGGGGSYC EYPGERLYEN VRFDVNGNSL 120 DEYSSDVTTL PGLKPREEYQ PSGGGGSGGG GSGGGGSLCN IHDLHKPHQS KPILTDENDT 180 QRTCSHTNPG GGGSGGGGSG GGGSMGGSTS KNSFKNTTNI ISNSIFNQMQ SCISMLDGKN 240 YIGVFGDGNI LNHVFQDLNL SLNTSCVQKH VNEENFITNL SNQITQNLKD QEVALTQWMD 300 AGTHDQKTDI EENIKVNLTT TLIQNCVSSL SGMNVLVVKG NGNIVENATQ KQSQQIISNC 360 LQGSKQAIDT TTGITNTVNQ YSHYTSKNFF DFIADAISAV FK 402 [0141] Sequence Number (ID): 6 [0142] Length: 315 [0143] Molecule Type: AA [0144] Features Location/Qualifiers: [0145] source, 1 . . . 315 [0146] >mol_type, protein [0147] >note, Recombinant ASFV antigen fusion proteins CPE [0148] >organism, synthetic construct [0149] Residues:
TABLE-US-00007 MDYWVSFNKT IILDSNITND NNDINGVSWN FFNNSFNTLA TCGKAGNFCE CSNYSTSIYN 60 ITNNCSLTIF PHNDVFDTTY QVVWNQIINY TIKLLTPATP PNITYNCTNF LITCKKNNGT 120 NTNIYLNIND TFVKYTNESI LEYNWNNSNI NNFTATCIIN NTISTSNETT LINCTYLTLS 180 SNYFYTFFKL YELGGGGSGG GGSHMNKPIC YQNDDKIFYC PKDWVGYNNV CYYFGNEEKN 240 YNNASNYCKQ LNSTLTNNNT ILVNLTKTLN LTKTYNHESN YWVNYSLIKN ESVLLRDSGY 300 [0150] Sequence Number (ID): 7 [0151] Length: 5 [0152] Molecule Type: AA [0153] Features Location/Qualifiers: [0154] source, 1 . . . 5 [0155] >mol_type, protein [0156] >note, The sequence of the linker [0157] >organism, synthetic construct [0158] Residues:
TABLE-US-00008 GGGGS5 5 [0159] END