Composition for cooling skin comprising phellodendron bark extract, method of preparing the same, topical composition comprising the same and dermatologically acceptable carrier, and method of cooling skin by applying the same

Abstract

Provided are a composition for cooling skin comprising a Phellodendron bark extract, a method of preparing the composition, a skin-improving product comprising the composition and a method of cooling skin by applying the composition to a skin of a subject, wherein the skin needs cooling. The composition prevents a burning sensation in the skin and under the skin and reduces heat generated under the skin and on the skin. The composition includes a Phellodendron bark extract as a main ingredient and further includes an extract of one or more selected from the group consisting of Anemarrhena asphodeloides, Scutellaria baicalensis, Paeonia lactiflora, Dictamnus dasycarpus, Alumen, Dryobalanops aromatic and Mentha arvensis.

Claims

1. A method of preparing a plant extract comprising: (a) washing a plant with water, wherein the plant is Phellodendron bark; and (b) putting the washed plant and a first solvent at a volume ratio of 1:12 to 1:42 in a vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, and performing a vacuum extraction for 50-80 minutes at 60-80 C. to obtain a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor; and (c) optionally, removing the first solvent from the liquid extract to obtain a concentrated extract, wherein the distillation solution in (b) or the concentrated extract in (c) is the plant extract.

2. The method according to claim 1 further comprising: (d) adding Alumen to a second solvent in a volume ratio of 1:23 to 1:334 and performing a warm impregnation at 65-80 C. for 50-80 minutes and filtering to obtain a filtrate; and (e) mixing the filtrate of (c) with the distillation solution of (b) in a volume ratio of 5:1 to 1:1 (the distillation solution of (b): the filtrate of (d)) to prepare a stock solution, wherein the stock solution is the plant extract.

3. The method according to claim 1, wherein the first solvent is one or more selected from the group consisting of alcohol, glycols, oils and water.

4. The method according to claim 2, wherein the second solvent is one or more selected from the group consisting of alcohol, glycols, oils and water.

5. The method according to claim 1, wherein the plant of (a) further comprises one or more selected from the group consisting of Anemarrhena asphodeloides, Scutellaria baicalensis, Paeonia lactiflora, Dictamnus dasycarpus, Alumen, Dryobalanops aromatic and Mentha arvensis.

6. The method according to claim 1, wherein the plant of (a) further comprises Anemarrhena asphodeloides, Scutellaria baicalensis, Paeonia lactiflora, Dictamnus dasycarpus, Alumen, Dryobalanops aromatic and Mentha arvensis.

Description

DESCRIPTION OF THE DRAWINGS

(1) FIGS. 1 and 2 illustrate the conditions before and after the attachment of a patch pad (Ar1) comprising a concentrated extract of a composition for cooling skin according to the present disclosure, and a patch pad (Ar2) comprising a distillation solution of the composition, respectively; and

(2) FIG. 3 is a graph showing the experimental results of FIGS. 1 and 2.

(3) FIG. 4 is a photo showing the location of the body where thermometers were attached in Experimental Example 2.

(4) FIG. 5 is a graph showing the temperature change of Test Person A's testis.

(5) FIG. 6 is a graph showing the temperature change of Test Person B's testis.

(6) FIG. 7 is a photo showing the location of the body where the temperature was measured in Experimental Example 3.

(7) FIG. 8a is a graph showing the temperature change where water was sprayed in Experimental Example 3.

(8) FIG. 8b is a graph showing the temperature change where the spray solution was sprayed in Experimental Example 3.

DESCRIPTION OF EMBODIMENTS

(9) Hereinafter, specific embodiments of the present disclosure will be described in more detail.

(10) The present disclosure relates to a composition for cooling skin by using a Phellodendron bark extract including Phellodendron bark as a main ingredient, which is developed to prevent a burning sensation in the skin and under the skin and to reduce heat generated under the skin and on the skin, a method of preparing the same, a skin-improving product using the same and a method of cooling skin by applying the same to a skin of a subject in need thereof.

(11) Phellodendron bark, which may be a main component of the composition, is dried bark of Phellodendron amurense belonging to the family Rutaceae. The bark is stripped from the trunk of a tree to remove coarse bark or the bark is cut, and then dried in the sun to be used as a drug. According to Korean Traditional Herbal Medicine Volumes including the Korean Pharmacopeia, Phellodendron bark has a bitter flavor and is cold in nature, and has efficacies of dissipating heat away and eliminating dampness, detoxicating, and eliminating fever caused by blood deficiency, and has a bactericidal activity against E. coli, typhoid, and cholera bacteria and a strong antibacterial activity against Gram-positive bacteria and gonococcus.

(12) Further, berberine, a component of Phellodendron bark, has a strong local astringent action, and thus is used externally for itching, and also has efficacies of removing heat toxins of wounds or burns. As such, when the Phellodendron bark having the local astringent cooling action, the antimicrobial activity, the anti-inflammatory effect, and the effects of eliminating dampness and stopping itching is used as a main ingredient in the composition, a burning sensation in the skin and under the skin may be prevented and heat generated under the skin and on the skin may be reduced.

(13) Further, the Phellodendron bark is a natural medicine which has a bitter taste, is used as a drug for strengthening the digesting functions of the stomach, stopping diarrhea, and controlling the intestinal function, and has efficacies of reducing fever, detoxifying, soothing pain, etc.

(14) The objects of the present disclosure may be achieved by using only Phellodendron bark having the above efficacies. However, to enhance the effects, the Phellodendron bark may be used as a main ingredient, and one or more herbal medicines selected from the group consisting of Anemarrhena asphodeloides, Scutellaria baicalensis, Paeonia lactiflora, Dictamnus dasycarpus, Alumen, Dryobalanops aromatic, and Mentha arvensis may be further included in the combination, and all these herbal medicines may be included to prepare a composition.

(15) With regard to the efficacies of the above components, first, Anemarrhena asphodeloides has efficacies of cooling heat and dissipating heat away, and nourishing body fluid and moistening dryness, and in particular, Anemarrhena asphodeloides includes a large amount of ingredients that make the skin firm and elastic. Therefore, when it is used together with Phellodendron bark, their effects are strengthened by each other and thus efficacy thereof is maximized. It is preferable that Anemarrhena asphodeloides is primarily used together with Phellodendron bark.

(16) Further, Anemarrhena asphodeloides is used for reducing fever, quenching thirst, and treating symptoms of chest tightness and restless movements of the legs and arms, and has therapeutic effects on cough and dry cough, ache in the joints, fever that occurs like the high tide and low tide, and cold sweat by promoting production of body fluids.

(17) Scutellaria baicalensis has efficacies of cooling heat and drying dampness, eliminating heat and toxins, and stopping bleeding by cooling blood, and thus is used for endothelial injury, anticonvulsant, antifungal, and antimicrobial actions by making it into medicine by boiling.

(18) Further, Scutellaria baicalensis reduces excessive heat to improve symptoms of high body fever, severe chest and limb discomfort and thirst, and indistinct consciousness in severe cases, and exhibits a high antipyretic effect from high fever caused by cold to high fever caused by general infectious diseases. Scutellaria baicalensis removes ocular irritation and pain caused by excessive heat accumulated in the body, is used for hypertension, headache, nosebleed, and uterine bleeding, and also shows its efficacy on skin irritation and boils. Such efficacies are attributed to its cold nature, which reduces fever to relieve the symptoms. When Scutellaria baicalensis is taken during pregnancy, diseases occurring during pregnancy may be treated. Further, Scutellaria baicalensis quenches thirst caused by high fever, and relieves fever and pain in the joints of the whole body, and exhibits anti-inflammatory and anti-pathogenic actions, antipyretic, diuretic, and hypotensive actions, hypoglycemic and hypolipidaemic actions, and choleretic and sedative actions.

(19) Paeonia lactiflora has efficacies of cooling heat and cooling blood, removing blood stagnation and stopping a pain, and thus is used for disease symptoms such as ocular irritation, tumentia, furuncle, etc.

(20) Dictamnus dasycarpus has efficacies of reducing heat and detoxicating, and eliminating dampness and stopping itching, and thus is used for disease symptoms such as furuncle, itching, jaundice, etc.

(21) Alumen has an astringent action and has efficacies of drying dampness and stopping itching to remove discomfort and odor in the site of inflammation, and is used as an astringent agent for skin mucosal inflammation or used as a local astringent agent for rinsing oral cavity.

(22) Dryobalanops aromatic has efficacies of opening the sensory organs, clearing the mind, reducing fever, and removing pain, and thus shows sedative, analgesic, and preservative actions, and is used for the treatment of burns, eczema, and infectious corneitis and used to soften the skin.

(23) Mentha arvensis has efficacies of dispelling epidemic pathogenic factor of wind-heat, and cooling head and eyes, and functions to dilate capillaries of the skin and to promote secretion of sweat glands to remove heat, and therefore, it is used for the purpose of reducing fever and stopping itching.

(24) These medicines are natural medicines, and non-toxic to the human body when used externally, and a blend thereof, an extract stock solution thereof, or a distillation solution thereof may reduce heat under the skin and on the skin, and remove itching, odor while giving topical sensation of refreshment, and exhibit antimicrobial and sedative effects.

(25) In particular, to reduce heat in the testicles of men and baby boys and to obtain additional efficacies, the composition is extracted in the form of a high pressure boiled liquid extract, a distillation solution, a powder, etc., and mixed with a pharmaceutically acceptable carrier, and then prepared in the form of a powder, a gel, a liquid mixture, a high pressure boiled liquid extract, etc., which is used to prepare skin-cooling and skin-improving products such as a functional spray, an ointment, a gel, a cream, a powder, a patch pad, etc.

(26) When the composition is prepared in the form of a liquid, it is preferable that the composition is directly sprayed or applied onto the testicles or the skin, or onto underwear or a diaper that is in direct contact with the testicles in an amount of about 0.2-0.4 cc per application. When the composition is a powder, the composition is directly sprayed onto the testicles or the skin, or evenly applied onto underwear or diaper in an amount of about 0.5-1.0 g per application. In this case, the composition is preferably used as a wetting dressing by sweat, feces and urine, etc. It is preferable that when the composition is a gel, it is directly applied to the skin uniformly. The composition is an extract obtained by high pressure boiling of natural herbal medicines, and it is non-toxic to the human body and has no side-effects in use.

(27) When the above-described composition of the present disclosure is prepared by using only Phellodendron bark and Anemarrhena asphodeloides, the composition may include 30-80% by weight of Phellodendron bark and 20-70% by weight of Anemarrhena asphodeloides.

(28) When the composition is prepared by using all of Phellodendron bark, Anemarrhena asphodeloides, Scutellaria baicalensis, Paeonia lactiflora, Dictamnus dasycarpus, Alumen, Dryobalanops aromatic, and Mentha arvensis, the composition may preferably include 35-50% by weight of Phellodendron bark and Anemarrhena asphodeloides and 50-65% by weight of the other ingredients, Scutellaria baicalensis, Paeonia lactiflora, Dictamnus dasycarpus, Alumen, Dryobalanops aromatic, and Mentha arvensis.

(29) Further, the composition is prepared by using all of Phellodendron bark, Anemarrhena asphodeloides, Scutellaria baicalensis, Paeonia lactiflora, Dictamnus dasycarpus, Alumen, Dryobalanops aromatic, and Mentha arvensis, the composition may preferably include 15-35% by weight of Phellodendron bark, 10-23% by weight of Anemarrhena asphodeloides, 3-15% by weight of Scutellaria baicalensis, 12-25% by weight of Paeonia lactiflora, 7-15% by weight of Dictamnus dasycarpus, 12-25% by weight of Alumen, 0.7-5% by weight of Dryobalanops aromatic, and 1-7% by weight of Mentha arvensis.

(30) These composition ratios of the herbal medicines are obtained, based on the prescriptions of Korean Traditional Herbal Medicine Volumes and experimental results regarding the maximization and persistence of reducible temperature.

Mode of the Invention

(31) The present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, the present invention is not limited to these examples, and these Examples are not intended to limit the scope of the present invention.

(32) The following Examples, Experimental Examples and Preparation Examples relate to the preparation of a natural herb liquid extract capable of preventing a burning sensation in the skin and under the skin and reducing heat generated under the skin and on the skin.

(33) Conditions of the following Examples include washing 600-1,200 g of one or more plant(s) selected from the group consisting of Phellodendron bark, Anemarrhena asphodeloides, Scutellaria baicalensis, Paeonia lactiflora, Dictamnus dasycarpus, Alumen, Dryobalanops aromatic and Mentha arvensis with purified water, and then putting the washed plant in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 15-25 L of purified water, and performing low temperature vacuum extraction for 50-80 minutes at 60-80 C. to obtain a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and purified water is removed from the liquid extract to obtain a concentrated extract. Herein, 80-130 g of Alumen were added to 3-10 L of purified water and warm impregnation was performed at 65-80 C. for 50-80 minutes and filtered, and then a filtrate was mixed with the composition in a ratio of 2:1 to prepare a stock solution.

Example 1

(34) 1,000 g of Phellodendron bark was washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 2

(35) 600 g of Phellodendron bark and 400 g of Anemarrhena asphodeloides were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 3

(36) 500 g of Phellodendron bark, 100 g of Scutellaria baicalensis, and 400 g of Anemarrhena asphodeloides were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 4

(37) 300 g of Phellodendron bark, 200 g of Dictamnus dasycarpus, and 200 g of Anemarrhena asphodeloides were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 5

(38) 300 g of Phellodendron bark, 200 g of Paeonia lactiflora, and 200 g of Anemarrhena asphodeloides were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 6

(39) 300 g of Phellodendron bark, 200 g of Dictamnus dasycarpus, 200 g of Paeonia lactiflora, and 100 g of Anemarrhena asphodeloides were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 7

(40) 300 g of Phellodendron bark, 50 g of Scutellaria baicalensis, 150 g of Dictamnus dasycarpus, 150 g of Paeonia lactiflora, 100 g of Anemarrhena asphodeloides, 10 g of Mentha arvensis, and 20 g of Dryobalanops aromatic were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 8

(41) 300 g of Phellodendron bark, 50 g of Scutellaria baicalensis, 150 g of Dictamnus dasycarpus, 150 g of Paeonia lactiflora, 100 g of Anemarrhena asphodeloides, 10 g of Mentha arvensis, and 20 g of Dryobalanops aromatic were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

(42) Then, 100 g of Alumen was added to 5 L of purified water and warm impregnation was performed at 65-80 C. for 1 hour and filtered, and then a filtrate was mixed with the distillation solution in a ratio of 2:1 (the distillation solution:the filtrate) to prepare a stock solution.

Example 9

(43) 300 g of Phellodendron bark, 50 g of Alumen, 50 g of Scutellaria baicalensis, 150 g of Dictamnus dasycarpus, 150 g of Paeonia lactiflora, 100 g of Anemarrhena asphodeloides, 10 g of Mentha arvensis, and 20 g of Dryobalanops aromatic were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 10

(44) 300 g of Phellodendron bark, 50 g of Alumen, 30 g of Inula helenium, 50 g of Scutellaria baicalensis, 150 g of Dictamnus dasycarpus, 150 g of Paeonia lactiflora, 100 g of Anemarrhena asphodeloides, 10 g of Mentha arvensis, and 20 g of Dryobalanops aromatic were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 11

(45) 300 g of Phellodendron bark, 50 g of Alumen, 30 g of Inula helenium, 50 g of Lonicera japonica, 50 g of Scutellaria baicalensis, 150 g of Dictamnus dasycarpus, 150 g of Paeonia lactiflora, 100 g of Anemarrhena asphodeloides, 10 g of Mentha arvensis, and 20 g of Dryobalanops aromatic were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 12

(46) 300 g of Phellodendron bark, 50 g of Alumen, 30 g of Inula helenium, 50 g of Lonicera japonica, 100 g of Eclipta prostrata, 50 g of Scutellaria baicalensis, 150 g of Dictamnus dasycarpus, 150 g of Paeonia lactiflora, 100 g of Anemarrhena asphodeloides, 10 g of Mentha arvensis, and 20 g of Dryobalanops aromatic were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

Example 13

(47) 300 g of Phellodendron bark, 60 g of Scutellaria baicalensis, 150 g of Paeonia lactiflora, 10 g of Mentha arvensis were washed with purified water, and then put in a super fast low temperature vacuum extractor which contains a liquid phase extractor and a gas phase distillation part, together with 20 L of purified water, and low temperature vacuum extraction was performed for 1 hour at 60-80 C. to obtain 10 L of a distillation solution in the gas phase distillation part and a liquid extract in the liquid phase extractor, and the purified water is removed from the liquid extract to obtain 5 L of a concentrated extract.

(48) In Examples 1-13, Glycyrrhiza uralensis FISCH may be further added in an amount of 3-15% by weight in the plants before washing with purified water.

(Experimental Example 1) Test of Patch Pads Comprising the Concentrated Extract or the Distillation Solution Obtained in Example 7

(49) The experimental results regarding patch pads comprising the concentrated extract or the distillation solution obtained in Example 7 are illustrated in FIGS. 1 to 3.

(50) Ar1 is a patch pad comprising the concentrated extract obtained in Example 7, Ar2 is a patch pad comprising the distillation solution obtained in Example 7, and FIGS. 1 and 2 illustrate the conditions before and after attachment of the patch pads, respectively, and FIG. 3 is a graph showing the results.

(51) The experimental results are as follows.

(52) 1) All, 31.4 C., before attachment of the patch pads;

(53) 2) Ar127.7 C., Ar228.4 C. upon detachment at 10 minutes after attachment of the patch pads; and

(54) 3) Ar129.5 C., Ar229.8 C. upon detachment at 25 minutes after attachment of the patch pads.

(55) Referring to the above experimental results, when the patch pad (Ar1), to which the concentrated extract of the above natural material including Phellodendron bark of the present invention was applied, was attached on the skin of which temperature was 31.4 C. before attachment, the temperature decreased to about 27.9 C. immediately after attachment, and 10 minutes later, the temperature further decreased to about 27.7 C. Then, when the patch pad was detached, the temperature of the skin gradually increased over time, but the temperature between 10-25 minutes after detachment was maintained at about 29.5 C., as shown in the graph of the experimental data.

(56) When the patch pad (Ar2), to which the distillation solution was applied, was attached on the skin of which temperature was 31.4 C. before attachment, the temperature decreased to about 27.7 C. immediately after attachment. The temperature of the skin gradually increased over time after attachment of the patch pad, and 10 minutes later, the temperature increased to about 28.4 C. Even after detachment of the patch pad, the temperature rise was maintained over time. However, the temperature between 10-25 minutes after detachment was maintained at about 29.8 C., as shown in the application test of the concentrated extract.

(Experimental Example 2) Test of a Spray Solution Comprising the Distillation Solution Obtained in Example 9

(57) A spray solution comprising the distillation solution obtained in Example 9 was used in this Experimental Example.

(58) A thermometer was attached at a lower side of testis of each of Test Persons A and B as shown in FIG. 4 and Test Persons A and B have worn underwear and pants and stayed for 30 minutes. Then, Test persons A and B took off pants and underwear, and the spray solution was sprayed on the lower side of testis of each of Test Persons A and B that the thermometer is attached. Then, Test Persons A and B have worn underwear and pants again and stayed for 90 minutes.

(59) The temperature of the testis of Test Person A measured during 120 minutes is shown in Table 1 and FIG. 5.

(60) TABLE-US-00001 TABLE 1 Start 10 min 20 min 30 min 31 min 35 min 40 min 50 min 35.76 C. 36.56 C. 36.93 C. 37.01 C. 36.42 C. 36.22 C. 35.94 C. 35.26 C. 60 min 70 min 80 min 90 min 100 min 110 min 120 min 35.29 C. 35.31 C. 35.21 C. 35.09 C. 35.02 C. 35.05 C. 35.00 C.

(61) The temperature of the testis of Test Person B measured during 120 minutes is shown in Table 2 and FIG. 6.

(62) TABLE-US-00002 TABLE 2 Start 10 min 20 min 30 min 31 min 35 min 40 min 50 min 36.73 C. 37.28 C. 37.57 C. 37.63 C. 37.25 C. 37.12 C. 36.76 C. 35.58 C. 60 min 70 min 80 min 90 min 100 min 110 min 120 min 35.00 C. 34.52 C. 34.68 C. 34.88 C. 34.70 C. 34.73 C. 34.83 C.

(63) As shown in Tables 1 and 2 and FIG. 6, in both Test Persons A and B, the temperature of the testis has increased gradually while wearing underwear and pants (0 to 30 minutes), but the temperature of the testis has quickly decreased after spraying the solution (30 minutes) and the temperature around 35 C. has been maintained for two hours.

(64) From the results above, it was confirmed that the composition according to this disclosure is effective in cooling the temperature of the testis while wearing underwear and pants and such an effect lasts for a long period.

(Experimental Example 3) Comparison Test Between Water and the Distillation Solution Obtained in Example 12

(65) A spray solution comprising the distillation solution obtained in Example 12 was used in this Experimental Example and its cooling effect was compared with that of water.

(66) The spray solution was sprayed on the right arm at the location shown in FIG. 7 and water was sprayed on the left arm at the location shown in FIG. 7 and Test Persons A, B and C participated in the experiment.

(67) The Temperature of the arms of each Test Person was measured right before the spraying and until 40 minutes after the spraying and is shown in Table 3 and FIG. 8a (water) and FIG. 8b (spray solution).

(68) TABLE-US-00003 TABLE 3 Before spraying 0 3 6 9 12 15 18 21 24 27 30 33 36 40 Water A 34.2 31.8 28.6 30.3 31 31.4 32 32.2 32.1 32.4 33.1 33.5 33.4 33.8 34 B 33.7 32.5 29.5 32.4 32.7 32.8 33.5 33.6 33.9 34 34.2 34.4 34.3 34.4 34.5 C 34.3 32 29.5 31.4 32.5 33.2 33.7 33.6 33.7 33.8 33.7 33.9 33.8 33.9 33.9 Spray solution A 33.5 32.1 28.5 31.5 31.9 31.5 31.5 31.6 31.6 31.7 31.8 31.8 31.6 31.7 31.8 B 34.1 32 29.6 28.4 29.4 30.8 31.4 31.4 34.7 31.8 31.6 31.8 31.9 32 32 C 34.2 29.6 29.6 28.2 29.5 30.8 31.5 31.7 31.6 31.8 31.7 31.8 31.8 31.9 32

(69) As shown in Table 3 and FIG. 8a and FIG. 8b, after spraying water, the temperature has decreased temporarily but quickly increased again to the similar temperature with the temperature before spraying. However, after spraying the spray solution, the temperature has decreased and then increased as time goes by, but maintained the lower temperature than the temperature before spraying.

(70) From the results above, it was confirmed that the composition according to this disclosure is effective in cooling the skin temperature.

(Preparation Example 1) Hydrogel Soothing Mask

(71) A hydrogel soothing mask was prepared including the following ingredients.

(72) Water, Dipropylene Glycol, Glycerin, Phellodendron Amurense Bark Extract, Niacinamide, Paeonia Lactiflora Bark/Sap Extract, Scutellaria Baicalensis Root Extract, Caprylic/Capric Triglyceride, Ceratonia Siliqua Gum, 1,2-Hexanediol, Chondrus Crispus Powder, Mentha Arvensis Extract, Cetyl Ethylhexanoate, Butylene Glycol, Glyceryl Stearate, Paeonia Suffruticosa Root Extract, Centella Asiatica Extract, Cellulose Gum, Sodium Polyacrylate, Allantoin, Chamomilla Recutita (Matricaria) Flower Extract, Hydrogenated Polydecene, Glyceryl Caprylate, Polysorbate 20, Rehmannia Glutinosa Root Extract, Adenosine, Ethylhexylglycerin, Honey Extract, Trideceth-6, Glycyrrhiza Glabra (Licorice) Root Extract, Sesamum Indicum (Sesame) Seed Extract, Sophora Flavescens Root Extract, Angelica Gigas Root Extract, Cimicifuga Racemosa Root Extract, Poria Cocos Extract, Morus Alba Bark Extract, Phellinus Linteus Extract, Rosa Multiflora Fruit Extract, Artemisia Princeps Leaf Extract, Panax Ginseng Root Extract, Paeonia Albiflora Root Extract, Hovenia Dulcis Fruit Extract, Polygonum Multiflorum Root Extract, Disodium EDTA, Fragrance

(Preparation Example 2) Lifting Mask

(73) A lifting mask was prepared including the following ingredients.

(74) Water, Butylene Glycol, Glycerin, Phellodendron Amurense Bark Extract, Paeonia Lactiflora Bark/Sap Extract, Scutellaria Baicalensis Root Extract, Glycereth-26, 1,2-Hexanediol, Mentha Arvensis Extract, Glyceryl Acrylate/Acrylic Acid Copolymer, PVM/MA Copolymer, Sodium Hyaluronate, PEG/PPG-17/6 Copolymer, Hydroxyacetophenone, Acrylates/C10-30 Alkyl Acrylate Crosspolymer, Cellulose Gum, Adenosine, Allantoin, Dipotassium Glycyrrhizate, Betaine, Polysorbate 80, Menthyl PCA, Ethyl Menthane Carboxamide, Tocopheryl Acetate, Tromethamine, Caprylyl Glycol, Ethylhexylglycerin, Disodium EDTA, Fragrance.

(Preparation Example 3) All in One Cream

(75) An all in one cream was prepared including the ingredients as follows.

(76) Phellodendron Amurense Bark Extract, Paeonia Lactiflora Bark/Sap Extract, Dictamnus Dasycarpus Root Extract, Anemarrhena Asphodeloides Root Extract, Eclipta Prostrata Extract, Scutellaria Baicalensis Root Extract, Mentha Arvensis Extract, Inula Helenium Extract, Cinnamomum Camphora (Camphor) Leaf Extract, 1,2-Hexandiol), Glycerin, Cyclopentasiloxane, PEG-30 Dipolyhydroxystearate, Glycereth-26, Macadamia Integrifolia Seed Oil, Cetearyl Glucoside, Pentaerythrityl Tetraethylhexanoate, Phytosqualane, Methyl Gluceth-20, Niacinamide, Cetyl Ethylhexanoate, Cetearyl Alcohol, Caprylic/Capric Triglyceride, Argania Spinsosa Kernel Oil, Methyl Glucose Sesquistearate, Glyceryl Stearate, PEG-100 stearate, Cetearyl Olivate, Sorbitan Olivate, Stearic acid, Butyrospermum oarkii (Shea Butter), Ammonium Acryloydimethyltaurate/VP Copolymer, Xanthan Gum, Caprylhydroxamic Acid, Caprylyl Glycol, Tocopheryl Acetate, Fragrance, Adenosine.

(Preparation Example 4) UV Protection Cream

(77) An UV protection cream was prepared including the following ingredients. Phellodendron Amurense Bark Extract, Paeonia Lactiflora Bark/Sap Extract, Ethylhexyl Methoxycinnamate, Dictamnus Desycarpus Root EXTRACT, Cyclopentasiloxane, Dicaprylyl Carbonate, Octocrylene, Caprylic/Capric Triglyceride, Glycyrhiza Glabra (Licorice) Root Extract, Anemarrhena Asphodeloides Root Extract, Eclipta Prostrata Extract, 1,2-Hexanediol, Scutellaria Baicalensis Root Extract, Inula Helenium Extract, Mentha Arvensis Extract, Dimethicone, Cetyl PEG/PPG-10/1 Dimethicone, Plantago Asiatica Extract, Lonicera Japonica (Honeysuckle) Flower Extract, Niacinamide, Butyl Methoxydibenzoylmethane, Glycerin, Butylene Glycol, Cinnamomum Camphora (Camphor) Leaf Extract, Titanium dioxide, Bis-Ethylhexyloxyphenol methoxyphenyl Triazine, Methylene Bis-Benzotriazolyl Tetramethylbutylphenol, Sodium Chloride, Adenosine, Tocopheryl Acetate, Centella Asiatica Extract, Menthol, Madecassoside, Sodium Hyaluronate, Sorbitan Olivate, Glyceryl Bhehenate/Eicosadioate, Cetyl Dimethicone, Stearalkonium Hectorite, Ceresin, Dimethicone/Vinyl Dimethicone Crosspolymer, Propylene Carbonate, Xanthan Gum, Polymethyl Methacrylate, Polyhydroxystearic Acid, Dimethicone/PEG-10/15 Crosspolymer, Fragrance, Disodidum EDTA.

(Preparation Example 5) Mist

(78) A mist was prepared including the following ingredients.

(79) Water, Phellodendron Amurense Bark Extract, Paeonia Lactiflora Bark Extract, Dictamnus Desycarpus Root Extract, Alumimum Sulfate, Anemarrhena Asphodeloides Root Extract, Eclipta Prostrata Extract, Glycyrrhiza Glabra (Licorice) Root Extract, Scutellaria Baicalensis Root Extract, Mentha Arvensis Extract, Inula Helenium Extract, Lonicera Japonica (Honeysuckle) Flower Extract, Plantago Asiatica Extract, Cinnamomum Camphora (Camphor) Leaf Extract, Butylene Glycol, Fragrance.

(80) Taken together, the present disclosure demonstrates that the composition functions to prevent a burning sensation in the skin and under the skin and to reduce heat generated under the skin and on the skin.