MATERIALS AND METHODS FOR ALGAL INOCULATION TO EFFECT DIRECT CAPTURE OF CARBON DIOXIDE FROM AIR

Abstract

The invention features a low cost nature-based carbon sequestering vaccine presenting capacity to restore global Carbon Dioxide to pre-industrial levels over a short, perhaps singly decade use across multiple deployments. This low tech, low cost, high impact, 100% nature based solution is carbon negative during its production and throughout deployment using multiple embodiments that capture and sequestrate carbon dioxide from at least a kiloton approaching a gigaton or beyond scale. The invention uses already developed technologies and biological methods in producing a vaccine for inoculating selected unpopulated or sparsely populated geozones with low potentials for disrupting human activities. Embodiments feature deployment of the vaccine into extreme cold environments with minor or non-existent negative externalities. Extremophilic algal cohorts are selectively adapted by growth in serial culture for spreading at sites where the algae growth and turn carbon dioxide from ambient air into biomass that sequesters the carbon for centuries.

Claims

1. A carbon sequestering composition of matter comprising: a) a cultured cocktail of extremophile psychrophile micro-organisms selected for enhanced growth and proliferation in extreme cold conditions; b) feed and mineral accelerants; and c) a container that stores and releases a) and b).

2. The composition of matter of claim 1 wherein said cocktail comprises a plurality of extremophile species.

3. The composition of matter of claim 1 wherein said extremophile psychrophile micro-organisms are selected from at least one genus selected from the group consisting of: Chlamydomonadales (Chlorophyta), and Zygnematales (Streptophyta).

4. The composition of matter of claim 1 wherein said extremophile psychrophile micro-organisms are selected from at least one species selected from the group consisting of: Chlainomonas kolii, Chlainomonas rubra, Chlamydomonas nivalis, Chloromonas pichinchae, Chloromonas sp., Raphidonema tatrae, Chromulina chionophila, and Cryptomonas frigoris.

5. The carbon sequestering composition of matter of claim 1 wherein said a) and said b) are in the form of a powder.

6. A machine for manufacture of carbon sequestering composition of matter of claim 1, said method comprising: a) a culture vessel that holds a cocktail of extremophile psychrophile micro-organisms at extreme cold conditions; b) a port for introducing CO.sub.2 into said vessel; c) nutrients and minerals that act to feed and to accelerate growth and propagation of said extremophile psychrophile micro-organisms; and d) a container that stores and releases each of a) and b) upon signal.

7. The machine of claim 6 wherein said port accepts waste from an industrial plant.

8. The machine of claim 6 fitted in a box adapted for transport on a truck platform.

9. The machine of claim 6 fitted in a box adapted for transport in a 20 shipping container.

10. The machine of claim 6 wherein said industrial plant includes a fermenting process that emits CO.sub.2.

11. A method for preparing a carbon sequestering psychrophile algae comprising: a) serial culture of said psychrophile algae for increased growth compared to said psychrophile algae used to feed said serial culture.

12. The method of claim 11 wherein said preparation of psychrophile algae comprises a plurality of psychrophile algae species.

13. The method of claim 11 wherein said culture features agar slopes.

14. The method of claim 11 wherein said microorganisms are cultured to form a concentration of about 60,000 to 10.sup.6 cells/ml.

15. The method of claim 11 wherein said microorganisms are cultured to form a concentration of about 60,000 to 10.sup.6 cells/ml.

16. The method of claim 11 further comprising freeze drying the product of a).

17. A cultured extremophile psychrophile micro-organism adapted for enhanced growth and proliferation on an ice surface in a dry granular form, said growth and proliferation resulting in CO.sub.2 capture from ambient air.

Description

EXAMPLES

[0054] Specific species of psychrophile algae were initially chosen for the initial vaccine trial product. Samples of wild type Antarctic snow algae: Chlamydomonas nivalis (CN) and Chloromonas pichinchae (CP) were purchased from UTEX in Texas, US. Proof of durability was seen when the purchased products were impounded by authorities for over ten days. Viability of the cultures was maintained even after the low pressures and temperatures in the plane cargo hold and normal storage conditions during the impoundment period.

[0055] The algae were serially cultured in 3N-BBM+V medium from a 1000 ml CCAP prepared from stock solutions:

TABLE-US-00001 NaNO.sub.3 25.0 g CaCl.sub.22H.sub.2O 2.5 g MgSO.sub.47H.sub.2O 7.5 g K.sub.2HPO.sub.43H.sub.2O 7.5 g KH.sub.2PO4 17.5 g NaCl 2.5 g [0056] with trace elements prepared in a stock of 0.75 g EDTA in 1000 ml distilled water added in the exact following sequence:

TABLE-US-00002 FeCl.sub.36H.sub.2O 97.0 mg MnCl.sub.24H.sub.2O 41.0 mg ZnCl.sub.2 5.0 mg CoCl.sub.26H.sub.2O 2.0 mg Na.sub.2MoO.sub.42H.sub.2O 4.0 mg [0057] and two vitamin supplements:

TABLE-US-00003 Thiaminhydrochloride (B1) 120 mg in 100 ml distilled water-sterile filtered Cyanocobalamin (B.sub.12) 100 mg in 100 ml distilled water then diluted: 1 ml into 99 ml distilled water-sterile filtered

[0058] From these stocks, the 3N-BBM+V medium is made by combining:

TABLE-US-00004 NaNO.sub.3 30.0 ml CaCl.sub.22H.sub.2O 10.0 ml MgSO.sub.47H.sub.2O 10.0 ml K.sub.2HPO.sub.43H.sub.2O 10.0 ml KH.sub.2PO.sub.4 10.0 ml NaCl 10.0 ml trace elements 6.0 ml B.sub.1 1.0 ml B.sub.12 1.0 ml

[0059] CN and CP samples were then cultured on agar slopes and phial solutions to obtain desired quantities and concentrations 60,000 to 10.sup.6 cells/ml for viability testing.

[0060] While the inventor used the term we or our in describing this invention, it is to be understood that the inventor conceived the claimed inventions. The inventor directed associates in developing and practicing the processes described herein. Associates also confirmed as second eyes reported observations.