AGRICULTURAL COMPOSITION FOR INCREASING CROP YIELDS

Abstract

The invention relates to a composition comprising at least one phytosteroids component, a fulvic acid component, a hydroxyacids component, a carotenes component, and an ionic liquids component.

Claims

1. An agricultural composition for increasing yield of crops on which the composition is applied to, comprising at least the following active components: a. A phytosteroids component, b. A fulvic acid component, c. A hydroxyacids component, d. A carotenes component, e. A source of ionic liquid.

2. The agricultural composition according to claim 1, wherein the phytosteroids component is 10-30% (w/v), of the total composition.

3. The agricultural composition according to claim 1, wherein the fulvic acid component is 10-40% (w/v), of the total composition.

4. The agricultural composition according to claim 1, wherein the hydroxyacids component is 1-20% (w/v), of the total composition.

5. The agricultural composition according to claim 1, wherein the carotenes component is 1-20% (w/v), of the total composition.

6. The agricultural composition according to claim 1, wherein the source of ionic liquid is 40-60% (w/v), of the total composition.

7. The agricultural composition according to claim 1, wherein the phytosteroids component corresponds to a plant extract enriched in phytosteroids.

8. The agricultural composition according to claim 7, wherein the phytosteroids component is obtained from an extract of the following selected plants: Quillaja saponaria, Phoenix dactylifera, Lolium perenne, Oryza sativa, Phalaris canariensis, Secal cereale, Triticum astivum, Zea mays, Lilium spp, Erythronium japanicum, Tulipa gesnerian, Typha latifo, Alnus glutinosa, Cannabis sativa, Gypsophilla perfoliata, Lychnis viscaria, Beta vulgaris, Castanea crenata, Fagopyrum seculentum, Rheum rhabarbarum, Apium, graveolens, Dauca spp, Arabodpsis thaliana, Brassica spp, Raphanus sativus, Cassia spp, Dolichos sp, Robinia sp, Acacia, Vicia faba, Phaseolus vulgaris, Eucalyptus, Citurs, Eriobotrya japonica, Thea sinensis, Helianthus annuus, Solidago altissima, Echium plantagineum, Pharbitis purpurea, Cucurbita moschata, Perilla frutescens, Nicotiana tabacum, Lycopersicon esculentum, Cupressus arizonica, Pinus spp, Cryptomeria japonica, Picea spp, Ginko biloba, Avena, Zea mays, Broccoli, Cauliflower, potato, oranges, apples, Brussels sprouts, onions, beans, Marchantia polymorphia amongst others but not limited to these plants.

9. The agricultural composition according to claim 1, wherein the phytosteroids component is obtained using an aqueous extraction method, comprising the following steps: a. Grinding selected plant leaves; b. Performing an aqueous extraction with water at a temperature selected between 40 C. to 70 C., more preferably at 60 C., producing an aqueous solution; c. Filtering and concentrating the aqueous solution under vacuum conditions, producing a concentrate (dry extract); d. Performing an enzymatic hydrolysis, using a estearase enzyme, producing the final phytosteroids component.

10. The agricultural composition according to claim 1, wherein the fulvic acid component is obtained from composting plant material.

11. The agricultural composition according to claim 10, wherein the plant material is selected among plant residues.

12. The agricultural composition according to claim 11, wherein the plant material is obtained from: composted material of tea, coffee, sugar cane.

13. The agricultural composition according to claim 10, wherein the plant residue is a vermin-compost.

14. The agricultural composition according to claim 13, wherein the vermin-compost is subjected to a liquid extraction producing liquid humus which is filtered, and thus is produced the fulvic acid component.

15. The agricultural composition according to claim 1, wherein the hydroxyacids component is preferably a source of -hydroxyacid (BHA).

16. The agricultural composition according to claim 15, wherein the source of BHA is obtained from a vegetal extract, more specifically from a plant selected among Schinus molle, Salix spp.

17. The agricultural composition according to claim 16, wherein the source of BHA is obtained by an aqueous extraction of a plant selected among Schinus molle, Salix spp, which have been ground.

18. The agricultural composition according to claim 17, wherein the aqueous extraction is performed at a temperature of between 80 C. and 120 C. producing an infusion, and wherein said infusion is further filtered and later lyophilized obtaining the source of BHA.

19. The agricultural composition according to claim 1, wherein the carotenes component is obtained from an aqueous extraction of carotene-rich plants, selected from tomato, peppers, guavas, banana, carrots, pine apples plums, mangoes, nectarines, orange, peach, papaya, apricots, melons, watermelons, squash, pumpkin, sweet potatoes, spinach, corn, passion fruit, red palm oil.

20. The agricultural composition according to claim 19, wherein the aqueous extraction is lyophilized and the resulting material is subjected to a second extraction step.

21. The agricultural composition according to claim 20, wherein the second extraction step is a supercritical fluid extraction.

22. The agricultural composition according to claim 21, wherein the supercritical fluid extraction is performed using carbon dioxide as solvent, and the process is performed at a pressure of 5 to 15 MPa, more preferably at 10 MPa, producing the carotenes component.

23. The agricultural composition according to claim 1, wherein the ionic liquid is obtained by mixing liquid humus, lecithin and water.

24. The agricultural composition according to claim 23, wherein the source of ionic liquid is of a size optimal for industrial uses.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0035] As previously stated, the present invention corresponds to a composition for the treatment of crop plants. Among the advantages of the composition of the present invention, the following can be noted: [0036] Increases yield of plants treated in the correct way [0037] Actively protects plants against different stresses [0038] The plant can use the different components of the novel composition of the present invention either for root and shoot development, flowering, fruit and tuber growth, general health status, among others. [0039] The composition is water-soluble and as such, it does not require organic solvents which may have negative effects in the environment, and can be applied for irrigation or through foliar application; [0040] The composition is completely innocuous for humans, animals, and the environment, wherein stock solutions can be disposed freely without damage to the environment; [0041] The composition does not include elements toxic to humans or animals, and as such, it can be applied with the presence of operators. [0042] The composition is easily absorbed by the plant. [0043] The crop suffers less damage by oxidation of lipid bilayer membranes and thus, maintains a better gas and metabolites exchange and therefore metabolic processes are more efficient. [0044] Increase in the efficiency of self-defense in situations of biotic and abiotic stress allows the plant to be able to reduce the use of energy and direct it to the production of tissues for growth and reproduction.

[0045] The novel composition of the present invention comprises at least but is not limited to the following active components:

[0046] i. A phytosteroids component,

[0047] ii. A fulvic acid component,

[0048] iii. A hydroxyacids component,

[0049] iv. A carotenes component,

[0050] v. An ionic liquids component.

[0051] In a more specific embodiment, the percentage of each component of the composition of the present invention is specified in Table 1.

TABLE-US-00001 TABLE 1 Percentage composition (w/v) of each of the components of the composition of the invention % Percentage % Preferred composition percentage Component (w/v) range composition (w/v) Phytosteroids component 10-30 20 Fulvic acid component 10-40 25 Hydroxyacids component 1-20 5 Carotenes component 1-20 5 Source of ionic liquid 40-60 50

[0052] Phytosteroids Component

[0053] In a more specific embodiment, the phytosteroids component including brassinosteroids corresponds to extract enriched in phytosteroids. In a non-limiting embodiment, the phytosteroids component can be obtained from many plant species. An example are extracts of the following selected plants:

[0054] Quillaja saponaria, Phoenix dactylifera, Lolium perenne, Oryza sativa, Phalaris canariensis, Secal cereale, Triticum astivum, Zea mays, Lilium spp, Erythronium japanicum, Tulipa gesnerian, Typha latifo, Alnus glutinosa, Cannabis sativa, Gypsophilla perfoliata, Lychnis viscaria, Beta vulgaris, Castanea crenata, Fagopyrum seculentum, Rheum rhabarbarum, Apium, graveolens, Dauca spp, Arabodpsis thaliana, Brassica spp, Raphanus sativus, Cassia spp, Dolichos sp, Robinia sp, Acacia, Vicia faba, Phaseolus vulgaris, Eucalyptus, Citurs, Eriobotrya japonica, Thea sinensis, Helianthus annuus, Solidago altissima, Echium plantagineum, Pharbitis purpurea, Cucurbita moschata, Perilla frutescens, Nicotiana tabacum, Lycopersicon esculentum, Cupressus arizonica, Pinus spp, Cryptomeria japonica, Picea spp, Ginko biloba, Avena, Zea mays, Broccoli, Cauliflower, potato, oranges, apples, Brussels sprouts, onions, beans, Marchantia polymorphia amongst others but not limited to these plants.

[0055] Fulvic Acid Component

[0056] In a specific embodiment, the fulvic acid component can be obtained from a range of sources such as composting plant material, volcanic soil, other soils samples, marine sediments or agricultural soils amongst others. In an even more specific embodiment, the plant material that is composted is selected among a broad range of plant residues. In a specific embodiment, the plant material is obtained from tea, coffee, sugar cane, blackstrap molasses and other plants. In a more specific embodiment, the plant residue is a vermin-compost. In a more specific embodiment, the vermin-compost is subjected to a liquid extraction producing liquid humus which is filtered, and thus is produced the fulvic acid component.

[0057] Hydroxyacids Component

[0058] In a specific embodiment, the hydroxyacids component is preferably a source of -hydroxyacid (BHA). In an even more specific and non-limiting embodiment, the source of BHA is obtained from a plant extract, more specifically from a plant selected among Schinus molle, Salix spp various berries, papaya, pine apple but not limited to these plants as well as milk and yoghurt. In a particular embodiment, the source of BHA is obtained by an aqueous extraction of the previously mentioned plants that have been ground. In a specific embodiment, the aqueous extraction is performed at a temperature of between 80 C. and 120 C. producing an infusion. Said infusion is further filtered and later lyophilized obtaining the source of BHA.

[0059] Carotenes Component

[0060] In a specific embodiment, the carotenes component is obtained from an aqueous extraction of carotene-rich plants, such as for example tomato, peppers, guavas, banana, carrots, pine apples plums, mangoes, nectarines, orange, peach, papaya, apricots, melons, watermelons, squash, pumpkin, sweet potatoes, spinach, corn, passion fruit, red palm oil. amongst others but not limited to these plants. Said aqueous extraction is lyophilized and the resulting material is subjected to a second extraction step. In a specific embodiment, the second extraction step is a supercritical fluid extraction. In an even more specific embodiment, the supercritical fluid extraction is performed using carbon dioxide as solvent, and the process is performed at a pressure of 5 to 15 MPa, more preferably at 10 MPa, producing the carotenes component.

[0061] Source of Ionic Liquid

[0062] In a specific embodiment, the ionic liquid is obtained by mixing liquid humus, lecithin and water.

[0063] Method of Use

[0064] The mixture of the different components of the composition is prepared to generate a composition as indicated in the present invention. The composition can be applied as a foliar spray using conventional spray equipment as predetermined doses depending on the plant species or by irrigation. Application can be at several stages during the growth cycle of the crop, in particular at the onset of flowering, during flowering or close to harvest of the crop, i.e., during periods of high stress for the plants, starting from sowing until harvest. The doses used vary between different crops plants and need to be determined empirically.

[0065] In a more specific embodiment, the recommended dose is between 0.5 liters per hectare in crops and smaller fruit trees, and of 1 liter for greater fruit trees. In a further embodiment, the composition of the invention should be applied at least 3 times per each growing season, during pre-flowering time, during flowering time, and at the time of harvest.

[0066] The advantages that some selected farmers have observed and their effects are the following: [0067] Change of color of the crop toward a more intense green improving photo photosynthesis. [0068] Leaves more open with greater exposure to the sun showing less stress. [0069] Greater root growth, improving the absorption of nutrients. [0070] Greater flowering and crop uniformity, showing a strong and less stressed crop. [0071] Better health status of the crop with a lesser requirement of pesticides. [0072] Higher fruit setting, thus ensuring higher production. [0073] Absence of residues in the crop. [0074] Harvest with desired commercial size, uniformity and health. [0075] Rapid recovery from situations of biotic and abiotic stresses. [0076] Increased production

[0077] Unlike other products, the composition of the invention does not incorporate hormones or specific nutrients to act directly in certain metabolic processes that may or may not be required for the plant, but instead, provide a specific mixture of components that help to improve plant performance. This is the reason why the novel composition of the invention has such good results in different crops.

DESCRIPTION OF EMBODIMENTS

Example 1: Obtaining Each of the Components of the Composition of the Invention

Source and Extraction of Phytosteroids

[0078] 1. Grinding plant leaves [0079] 2. Performing an aqueous extraction with water at a temperature selected between 40 C. to 70 C., more preferably at 60 C., producing an aqueous solution; [0080] 3. Filtering and concentrating the aqueous solution under vacuum conditions, producing a concentrate (dry extract); [0081] 4. Performing an enzymatic hydrolysis, using an esterase enzyme, producing the final phytosteroids component.

[0082] In a more specific embodiment, the enzymatic hydrolysis is performed by diluting the dry extract in water, more preferably bi-distilled water, at a concentration of 0.5 to 2 g per 50 ml of water. An estearase solution at a concentration of 50 units/ml prepared in a buffered solution at pH 7.5 is added dropwise to the dry extract solution while stirring constantly, maintaining pH at 7.5 and a temperature between 15 C. and 25 C., more preferably at 22 C. After a period of 30 min to 100 min a white precipitate is obtained. The white precipitate is washed twice with bi-distilled water, and later is centrifuged for at least 15 min at 6,000 g. The concentrate is filtered and dried in an oven for at least 30 hours, more preferably 48 hours, at a temperature between 30 and 40 C., more preferably at 37 C.

[0083] Source and Extraction of Fulvic Acid

[0084] The fulvic acid component was obtained by composting residual plant material obtained from tea and coffee. Said plant residues were subjected to vermicomposting and later, the obtained compost was subjected to a liquid extraction. The obtained liquid was filtered and the fulvic acid component is the result of said filtration step.

[0085] Source and Extraction of Hydroxyacids

[0086] The source of BHA was obtained by an aqueous extraction of Schinus molle ground leaves at a temperature of 100 C. The resulting infusion was filtered and lyophilized producing the source of BHA.

[0087] Source and Extraction of Carotenes

[0088] Tomatoes and carrots were ground and mixed, and further subjected to an aqueous extraction. The resulting liquid extract was further lyophilized. The obtained dry powder was subjected to a supercritical carbon dioxide extraction at a pressure of 10 MPa. using conventional procedures.

[0089] Source of Ionic Liquid

[0090] The ionic liquid was obtained by mixing liquid humus, lecithin and water.

Examples: Evaluation of Effects in the Field of Treatment of Plants with the Composition

[0091] Standard methods are used for the evaluation of the physiological effects in the crop. Final crops yield and crop parameters were determined using standard method.

[0092] In the following examples, a composition according to the present invention was used, as the one described in Table 1, in the column corresponding to % Preferred percentage composition (w/v).

Example 2: Application of the Composition of the Invention to Cherry Trees General Data

[0093] An area of 2.8 ha of cherry in production planted with Regina and KORDIA varieties was divided so that part of the areas was not treated and the other treated with the composition. The total area treated with the composition of the invention for variety Regina was 0.64 hectares and for variety Kordia was 0.32 hectares. The rest was used as control.

[0094] Objectives

[0095] The purpose of this filed trial was to determine the impact of the application of the composition of the invention on cherries trees.

[0096] Materials and Method

[0097] There were two treatments T0=No application of composition of the invention and T1=Foliar application of the Composition of the invention. Applications were made every 10 days at a dose of 1 liter per hectare.

TABLE-US-00002 TABLE 2 Results of harvested yield of cherries from field trials With composition Without composition Variety (kg) (kg) Regina 11281 10564 Kordia 13400 11081

[0098] Results

[0099] The trees treated with the composition produced a greater yield of fruit in the range of 6-20% in comparison with untreated trees.

Example 3: Application of the Composition of the Invention to Fodder Crops General Information

[0100] The plot used in this assay comprised an area of approximately 22,000 hectares, in which the surface covered by the crops was an average of 500 hectares. Fodder crops used in the analysis were sugar beets and turnips.

[0101] Objectives

[0102] The purpose of this field trial was to determine the impact of the application of the composition of the invention on sugar beets and turnips.

[0103] Materials and Method

[0104] The application of the composition of the invention was done via spraying, at a dose of 500 cc/Ha. Applications were repeated every 14 days until harvest.

[0105] The treatments were identified as T1=without the application of a composition of the invention and T2=with application of composition of the invention, each with 4 replicates per treatment. For the collection of data, a framework of 1 m.sup.2 was used chosen completely at random, for the areas under investigation in order to quantify crop production.

[0106] All the plant material of the experimental unit (1 m.sup.2) was removed, for further weight analysis. Also, the roots were grouped according to their size and were further categorized as small, medium, or large.

[0107] The beet sample was subjected to a measurement of dry matter, metabolizable energy and amount of fiber. Performance parameters were evaluated, and size in the beet crops and forage turnips. Two evaluations of yield (kg/m.sup.2) were conducted for beet, whereas for turnip one measurement was made.

[0108] The results of the measurements are detailed below:

TABLE-US-00003 TABLE 3 Results of harvested yield of sugar beets from first field trial With no Applying the composition of composition of the invention the invention Trial 1 Sample (T1) kg (T2) kg Beet 1 4.27 9.78 Beet 2 6.8 8.6 Beet 3 6.73 8.2 Beet 4 6.43 8.35 Average 6.06 8.73 Estimated production per hectare 60,575 87.325 % dry matter 13% 7,693 11,090 Increase 44%

[0109] Results

[0110] In the first trial of sugar beet, the obtained data showed that the treatment applied with novel composition of the invention (T2) yielded an estimated increase in the yield of 26,750 Kg. This represents a 44 percent increase of the productivity over the treatment without the composition of the invention.

TABLE-US-00004 TABLE 4 Results of harvested yield of sugar beets from second field trial With no Applying the composition of composition of the invention the invention Trial 2 Sample (T1) kg (T2) kg Beet 1 6.0 12.5 Beet 2 9.9 12.0 Beet 3 10.1 12.8 Beet 4 8.3 18.4 Beet 5 14.5 8.1 Average 9.76 12.76 Estimated production per hectare 97,600 127,600 % dry matter 13% 12,395.20 16,205.20 Increase 44%

[0111] Results

[0112] As in the trial 1, the increase in yield of the treatment with the composition of the invention represents a 44 percent of the production obtained with the treatment without the composition of the invention.

TABLE-US-00005 TABLE 5 Results of harvested yield of turnips from field trials With no Applying the composition of composition of Trial 1 Sample the invention the invention Turnip 1 14.3 10.3 Turnip 2 10.6 16.1 Turnip 3 10.3 13.2 Turnip 4 11.9 21.8 Average 11.78 15.35 Estimated production per hectare 117,750 153,500 % dry matter 13 14,954.25 19,494.50 Increase 30%

[0113] Results

[0114] In the first trial of turnip, treatment with application of the composition of the invention showed an increase in 30% in yield.

Example 4: Application of the Composition of the Invention to Potato Crops General Information

[0115] Mode of Application and Dosage

[0116] The application of the composition of the invention is done by foliar application, with conventional spraying equipment.

[0117] Previous tests carried out with the composition of the invention in potatoes have determined that the optimal dosage to be used is 0.5 liters per hectare for foliar applications independent of the irrigation level used in the field.

[0118] Applications were performed every 10-15 days.

[0119] Objective of Field Trial:

[0120] The purpose of this field trial was to determine the impact of the application of the composition of the invention on potatoes.

[0121] Time of Application

[0122] The following table shows the times of application for the potato crop.

TABLE-US-00006 TABLE 6 Timing of application of novel composition to potatoes Phenological Dose Application Effects to be stage Product (cm.sup.3/ha) route Objectives observed Flowering start Novel 500 cc Foliar To reduce the Uniform composition application effect of stress Flowering of the generated by this invention process. Full Bloom Novel 500 cc Foliar Reduce the effect Uniform composition application of stress Flowering of the generated by this invention process. Filling of tubers Novel 500 cc Foliar Improve root and Leaves of intense composition application vegetative growth green color and of the Improve stress best invention condition photosynthetic Improve caliber leaf disposition. Increases the Tubers are larger resistance and more diseases uniform. Healthy Improve the plants and photosynthetic delayed capacity of the senescence plant

[0123] Results of Field Trials

[0124] Field trials were carried out in potato crops in two different locations in Chile.

[0125] Field Trial 1

[0126] Generalities

[0127] The study was conducted in a total of 220 hectares cultivated with potatoes. The soil is soil of volcanic origin. Potato variety planted was FL1856 provided by a local industrial customer.

[0128] Main Problem:

[0129] In the case of cultivation for industrial processes, two metrics are most relevant: first overall yield and secondly the size of the tuber, since small tubers (30 mm) are not harvested by machines, and excessively large tubers are not processed by the industry.

[0130] Objective of the Trial

[0131] The purpose of this test is to assess the effect of the application of the composition of the invention on potato crops.

[0132] Materials and Method

[0133] Treatments:

[0134] T1=commercial control standard treatment of the field, 3 hectares approximate surface treated.

[0135] T2=the composition of the invention for foliar application. Treatment with three applications, a) start of flowering; (b) end of flowering and (c) pre-harvest

[0136] Dose: 0.5 Liters per hectare

[0137] Method of application: Conventional spraying equipment Irrigation: According to usual field irrigation practices.

[0138] Design of Experiment: Two adjoining blocks of equal exposure, slope, and handling, planted on the same date with the same variety were chosen.

[0139] Each unit of 3 hectares corresponded to one treatment, one of them corresponds to the application of the composition of the invention and the other is used as a non-treated control.

[0140] During the stage of tuber filling and pre-harvest, three evaluations for each treatment were made, taking in every opportunity four samples corresponding to the four repetitions, each in turn composed of four contiguous plants in completely random locations.

[0141] Tubers were identified for each plant considered for the study, and measured the equatorial zone of each tuber and finally weighing them in an electric scale.

[0142] Aspects to be Evaluated:

a. Number of tubers formed, total weight (kg) per sample.

[0143] Analysis of Results

TABLE-US-00007 TABLE 7 Comparison of harvested yield of potatoes in first field trial Accumulated Weight (Kg) Weight (kg) WITH WITH- WITH WITH- Plot (T2) OUT (T1) Plot (T2) OUT (T1) 1a EV 08-02 17.8 11.9 1a EV 08-02 17.8 11.9 2a EV 02-03 28.9 21.2 2a EV 02-03 46.7 33.1 3a EV 30-03 25.18 23.8 3a EV 30-03 71.88 56.9 4a EV 08-05 35.35 24.85 4a EV 08-05 107.23 81.75

[0144] Result:

[0145] Treatment of the potato crop with the novel composition of the invention resulted in significant yield increases.

TABLE-US-00008 TABLE 8 Comparison of the number of tubers in first field trial No cumulative No. of tubers tubers WITH WITH- WITH WITH- Plot (T2) OUT (T1) Plot (T2) OUT (T1) 1a EV 08-02 153 130 1a EV 08-02 153 130 2a EV 02-03 151 121 2a EV 02-03 304 251 3a EV 30-03 149 192 3a EV 30-03 453 443 4a EV 08-05 172 135 4a EV 08-05 625 578

[0146] Results:

[0147] Treatment of the potato crop with the novel composition of the invention resulted in increased number of tubers.

[0148] Field Trial 2

[0149] Generalities

[0150] The study was conducted in a total of 800 hectares cultivated with potatoes. The soil is soil of volcanic origin. Potato variety planted was FL1856 provided by local industrial customer.

[0151] Main Problem:

[0152] In the case of cultivation for industrial processes, two metrics are most relevant: first overall yield and secondly the size of the tuber, since small tubers (30 mm) are not harvested by machines, and excessively large tubers are not processed by the industry.

[0153] Objective of the Test

[0154] Objective of field trial:

[0155] The purpose of this field trial was to determine the impact of the application of the composition of the invention on potatoes.

[0156] Materials and Method

[0157] Treatments:

[0158] T1=commercial control with standard treatment of field operations. An area consisting of approximately 3 hectares was used in the trial.

[0159] T2=the composition of the invention for foliar application. Treatment with three applications, a) start of flowering; (b) end of flowering and (c) pre-harvest in a 20 hectares block.

[0160] Dose: 0.5 Liters Per Hectare

[0161] Method of application: Conventional spraying equipment Irrigation: According to usual field practices

[0162] Design of Experiment:

[0163] Units of 20 hectares were established due to the capacity of the pond of the equipment, with the same characteristics, with seeds of the same variety and similar planting time, considering equal treatment except for the applications of the composition of the invention.

[0164] Each unit of 20 hectares corresponded to a treatment: first: no application of the novel composition of the invention; second: foliar application of the composition of the invention,

[0165] During the stage of filling of tubers and pre-harvest, three assessments for each treatment were made, taking in every opportunity four samples corresponding to the four repetitions, each in turn composed of four contiguous plants in completely random locations.

[0166] Tubers were identified for each plant considered for the study, and measured the equatorial zone of each tuber and finally weighing them in an electric scale.

[0167] Aspects to be evaluated: Number of tubers formed, Classification of each tuber by caliber, Total weight (kg) per sample.

[0168] Analysis of Results

TABLE-US-00009 TABLE 9 Comparison of harvested yield of potatoes in second field trial Accumulated Weight (kg) Weight (kg) WITH- WITH WITH- WITH Plot OUT (T1) (T2) Plot OUT (T1) (T2) 1a EV 24-01 12.7 13.55 1a EV 24-01 12.7 13.55 2a EV 08-02 14.4 20.32 2a EV 08-02 27.1 33.87 3a EV 02-03 16.9 28.9 3a EV 02-03 44.0 62.77

[0169] Result:

[0170] Treatment of the potato crop with the composition of the invention resulted in significant yield increases.

TABLE-US-00010 TABLE 10 Comparison of number of tubers in second field trial No cumulative No. of tubers tubers WITH- WITH WITH- WITH Plot OUT (T1) (T2) Plot OUT (T1) (T2) 1a EV 24-01 109 129 1a EV 24-01 109 129 2a EV 08-02 94 124 2a EV 08-02 203 253 3a EV 02-03 109 122 3a EV 02-03 312 375

[0171] Results:

[0172] Treatment of the potato crop with the novel composition of the invention resulted in increased number of tubers.

Example 5: Application of the Composition of the Invention to Tomatoes General Data

[0173] An area of 11.7 ha of tomatoes used for the production of processing tomatoes is divided so that part of the areas is not treated and the other is treated with the composition. The total area treated with the composition of the invention for variety is 7.05 hectares. The rest is used as control.

[0174] Objectives

[0175] The purpose of this field trial is to determine the impact of the application of the composition of the invention on tomato production. With and without standard use of field bioestimulants.

[0176] Materials and Method

[0177] There are three treatments TO (4.65 ha)=No application of composition of the invention, T1 (4.65 ha)=Foliar application of the Composition of the invention with standard treatment of field operations and T2(2.4 ha)=Foliar application of the Composition of the invention without addition conventional bio-stimulants.

[0178] Applications are made every 15 days at two doses of 0.5 (the first three) and 1 liter (two last ones) per hectare. Conventional spray equipment is used to deliver the composition of the invention.

[0179] The harvested yield (kg) of tomatoes treated with the composition is compared with the harvested yield (kg) of tomatoes without treatment.

TABLE-US-00011 TABLE 11 Comparison of harvested yield of tomatoes in field trial Average fruit Weight per Plant Total Weight Treatment (kg) (kg)/ha T0 control 1.27 83,672 T1 1.77 68,476 T2 2.00 70,663

[0180] Results

[0181] Treatment of tomato crop with the novel composition of the invention resulted in significant yield increases and a successful replacement of conventional bio-stimulants.

Example 6: Application of the Composition of the Invention to Grapes General Data

[0182] An area of 10 hectares of grapes used for the production of table grapes is divided so that part of the areas is not treated and the other is treated with the composition. The total area treated with the composition of the invention for grapes is 5 hectares. The rest is used as control.

[0183] Objectives

[0184] The purpose of this field trial is to determine the impact of the application of the composition of the invention on table grape production.

[0185] Materials and Method

[0186] There are two treatments T0=No application of composition of the invention and T1=Foliar application of the Composition of the invention. Application is made using conventional spray equipment. The following table shows the times of application for the table grape crop.

TABLE-US-00012 TABLE 12 Timing of application of novel composition to table grapes Phenological Dose Application Effects to be stage Product (cm.sup.3/ha) route Objectives observed Sprouting Novel 500 cc Foliar To reduce the effect Increase composition application of stress generated Sprouting of the by this process. invention Flowering start Novel 500 cc Foliar To reduce the effect Uniform (30-50%) composition application of stress generated Flowering of the by this process. invention Full Bloom Novel 1000 cc Foliar Reduce the effect of Uniform (70-100%) composition application stress generated by Flowering of the this process. invention Berry Growth Novel 500 cc Foliar Improve stress Uniform composition application condition Improve caliber of the cell elongation. invention Berry Growth Novel 500 cc Foliar Improve vegetative Uniform composition application growth caliber of the Improve stress Uniform color invention condition Healthy plants Improve caliber Improve color Increases the resistance diseases Pre-harvest Novel 1000 cc Foliar Improve soluble Early harvest composition application solids content Uniform of the Improve color caliber invention Increases the Uniform color resistance diseases Healthy plants

[0187] Aspects Evaluated:

[0188] Sprout growth (diameter and length)

[0189] Foliar area

[0190] Soluble Solid content (SSC)

[0191] Average grape cluster weight

[0192] Analysis of Results

TABLE-US-00013 TABLE 13 Comparison of table grape plant condition in field trial Leaf Foliar Foliar Length weight area area/ ssc Diameter (cm) (gr) (cm.sup.2) Length (Brix) T0 Control 0.77 156.5 65.06 780.7 5.13 15.20 T1 0.81 160.4 70.96 851.5 5.40 16.20

[0193] Results

[0194] Treatment of table grape with the novel composition of the invention resulted in a overall better condition.

TABLE-US-00014 TABLE 14 Comparison of harvested yield of table grapes from field trials Average Grape Cluster Weight Total Weight Treatment per Plant (kg) (kg)/ha T0 Control 23.46 16,750 T1 33.08 23,619

[0195] Results

[0196] Treatment of table grape with the novel composition of the invention resulted in resulted in significant yield increases.

Example 7: Application of the Composition of the Invention to Almonds Trees

[0197] General Data

[0198] An area of 5.3 hectares of almonds trees used for the production of almonds is divided so that part of the areas is not treated and the other is treated with the composition. The total area treated with the composition of the invention is 3.3 hectares. The rest is used as control.

[0199] Objectives

[0200] The purpose of this field trial is to determine the impact of the application of the composition of the invention on almond production.

[0201] Materials and Method

[0202] There are four treatments TO (2 ha)=No application of composition of the invention, T1(1.3 ha)=Irrigation system application of the Composition of the invention, T2(1 ha)=Foliar application of the Composition of the invention at 500 cc/ha dose and T3(1 ha)=Foliar application of the Composition of the invention at 1000 cc/ha dose. Foliar application of the composition is made with conventional spray equipment.

TABLE-US-00015 TABLE 15 Dose of application of the Composition of the invention in almonds in field trial Treatment Dose (cm.sup.3/ha) Area (ha) T0 Without Composition of the 2 invention T1 500 cc/ha 1.3 Irrigation system application (every 15 days) T2 500 cc/ha 1 Foliar application T3 1000 cc/ha 1 Foliar application

TABLE-US-00016 TABLE 16 Timing of Foliar application of novel composition to Almonds Phenological stage Product Objectives Effects to be observed Full Bloom Novel composition Improve Fruit Set Increase Fruit set (70-100%) of the invention Reduce the effect of stress generated by this process. Fruit Set Novel composition Improve caliber Increase caliber of the invention Improve Fruit retention Increase fruit number Increases the Healthy plants resistance diseases Pre-harvest Novel composition Improve caliber Uniform caliber of the invention Improve Fruit retention Healthy plants Increases the Increase fruit number resistance diseases

TABLE-US-00017 TABLE 17 Comparison of harvested yield of almond in field trial Treatment Average Weight per Tree (kg) Total Weight (kg)/ha T0 Control 12.4 5,902 T1 11.5 5,474 T2 13.5 6,426 T3 16.24 7,730

[0203] Results

[0204] Foliar treatment with a 1000 cc/ha dose in Almond trees with the novel composition of the invention resulted in resulted in significant yield increases.

Example 8: Application of the Composition of the Invention to Avocado Trees

[0205] General Data

[0206] An area of 2 hectares of avocado trees used for the production of avocado is divided so that part of the areas is not treated and the other is treated with the composition. The total area treated with the composition of the invention is 1 hectare. The rest is used as control.

[0207] Objectives

[0208] The purpose of this field trial is to determine the impact of the application of the composition of the invention on avocado production.

[0209] Materials and Method

[0210] There are two treatments T0=No application of composition of the invention and T1=Foliar application of the Composition of the invention. Applications are made every 15 days at a dose of 1 liter per hectare. Conventional spray equipment is used to deliver the composition of the invention.

[0211] Aspects Evaluated:

[0212] Frost damage

[0213] Harvested yield

TABLE-US-00018 TABLE 18 Comparison of frost damage in avocado trees in field trial Treatment Foliage damage T0 control 30-40% T1 1-5%

[0214] Results

[0215] Treatment of avocado trees with the novel composition of the invention resulted in an increase resistance to frost damage

TABLE-US-00019 TABLE 19 Comparison of harvested yield of avocado in field trial Average Weight per Tree Total Weight Percentage of Treatment (kg) (kg)/ha exportable fruit T0 Control 51.2 36,556 58.11% T1 72.13 51,500 70.61%

[0216] Results

[0217] Treatment of avocado trees with the novel composition of the invention resulted in resulted in significant yield increases.

Example 9: Application of the Composition of the Invention to Pomegranate

[0218] General Data

[0219] An area of 17 hectares of pomegranate trees used for the production of pomegranate is divided so that part of the areas is not treated and the other is treated with the composition. The total area treated with the composition of the invention is 2 hectares. The rest is used as control.

[0220] Objectives

[0221] The purpose of this field trial is to determine the impact of the application of the composition of the invention on pomegranate sweetness and acidity.

[0222] Materials and Method

[0223] There are two treatments T0=No application of composition of the invention and T1=Foliar application of the Composition of the invention. Applications are made at a dose of 1 liter per hectare. Conventional spray equipment is used to deliver the composition of the invention.

TABLE-US-00020 TABLE 20 Timing of Foliar application of novel composition to pomegranate trees Phenological Effects to be stage Product Objectives observed Flowering start Novel composition of Improve Fruit Set Increase Fruit set the invention Improve Fruit retention Reduce the effect of stress generated by this process. Fruit Set Novel composition of Improve caliber Increase caliber the invention Increases the Increase fruit resistance diseases number Healthy plants Fruit growth Novel composition of Improve sweetness Increase sweetness the invention Improve acidity Decrease acidity

TABLE-US-00021 TABLE 21 Comparison of pomegranate condition in field trial Acidity Caliber Sweetness (Titratable Brix/ Treatment (kg) brix acidity) acidity T0 Control 6.14 12.4 3.1 3.8 T1 6.28 15.1 2.6 5.6

[0224] Results

[0225] Treatment of pomegranate trees with the novel composition of the invention resulted in resulted in significant fruit quality increases (increasing sweetness and decreasing acidity as the variety required).

INDUSTRIAL APPLICABILITY

[0226] The composition of the present invention can be applied in agricultural industry.