Combination products and cosmetic compositions for controlling skin disorders and skin aging that affect keratinocytes and/or fibroblasts and the dermis

Abstract

This invention relates to a cosmetic combination product comprising a first cosmetic composition comprising glucosamine or one of the salts thereof, a second cosmetic composition comprising hyaluronic acid, and possibly a third cosmetic composition comprising collagen, a cosmetic composition comprising glucosamine or one of the salts thereof, hyaluronic acid, and possibly collagen, the uses thereof, in particular for inducing the synthesis of hyaluronic acid and as such combat aging of the skin affecting the keratinocytes and/or the fibroblasts and the dermis, as well as a cosmetic method that implements these combination products and compositions.

Claims

1. A method to induce the synthesis of hyaluronic acid which comprises administering orally or topically or both to an individual in need thereof; a cosmetic product combination comprising a first cosmetic composition comprising from 0.1% to 10% by weight of glucosamine sulfate, a second cosmetic composition comprising at least 4% by weight of hyaluronic acid and a third cosmetic composition comprising from 0.1% to 10′1% by weight of collagen, or a cosmetic composition comprising from 0.1% to 10% by weight of glucosamine sulfate, at least 4% by weight of hyaluronic acid and from 0.1% to 10% by weight of collagen, wherein the glucosamine sulfate, hyaluronic acid and collagen exhibit synergistic synthesis of hyaluronic acid by fibroblasts.

2. The method according to claim 1, for inducing epidermal hyaluronic acid synthesis, wherein the administering is to an individual in need thereof.

3. The method according to claim 1, for enhancing the synthesis of hyaluronic acid in the dermis and connective tissue of the dermal epidermal junction, wherein the administering is to an individual in need thereof.

4. The method according to claim 1, for combatting the signs of aging linked to the loss of connective tissue and skin, wherein the administering is to an individual in need thereof.

5. The method according to claim 1, for combatting the signs of aging linked to the loss of matrix tissue, dryness and dry and thin appearance of the skin, wherein the administering is to an individual in need thereof.

6. The method according to claim 1, for combatting the signs of aging linked to the loss of matrix tissue, the appearance of lines and wrinkles and sagging skin, wherein the administering is to an individual in need thereof.

7. The method according to claim 1, for combatting skin aging affecting the skin, wherein the administering is to an individual in need thereof.

8. The method according to claim 1, for enhancing the effects and duration of cosmetic treatment based on hyaluronic acid, wherein the administering is to an individual in need thereof.

9. The method according to claim 1, for enhancing skin hydration, wherein the administering is to an individual in need thereof.

10. The method according to claim 1, for preparing reconstructed skin and subcutaneous implants, wherein the administering is to an individual in need thereof.

11. The method according to claim 1, wherein said second cosmetic composition comprising hyaluronic acid and said third cosmetic composition comprising collagen constitute one cosmetic composition wherein the hyaluronic acid and collagen are derived from a cartilage extract.

12. The method according to claim 1, wherein the hyaluronic acid is of biotechnological origin.

13. The method according to claim 1, wherein the collagen is derived from a purification method from fish, mammal or poultry.

14. The method according to claim 1, wherein said compositions are administered orally.

15. The method according to claim 1, wherein said compositions are administered topically.

16. The method according to claim 1, wherein the composition comprising glucosamine sulfate is administered orally and the compositions comprising hyaluronic acid and/or collagen are administered topically.

17. The method according to claim 1, wherein the composition comprising hyaluronic acid and/or collagen are administered by the oral route and the composition comprising glucosamine sulfate is administered topically.

18. The method according to claim 1, wherein the compositions comprising glucosamine sulfate and/or collagen are administered orally and the composition comprising hyaluronic acid is administered topically.

19. The method according to claim 1, wherein the cosmetic combination product comprises 0 to 30% hyaluronic acid.

20. The method according to claim 1 wherein the cosmetic combination product is a cosmetic composition comprising glucosamine sulfate, hyaluronic acid and collagen.

21. The method according to claim 20, wherein the hyaluronic acid and collagen are derived from a cartilage extract.

22. The method according to claim 20, wherein the hyaluronic acid is of biotechnological origin.

23. The method according to claim 20, wherein the cosmetic composition is administered orally.

24. The method according to claim 20, wherein the cosmetic composition is administered topically.

25. The method according to claim 20, wherein the cosmetic composition comprises 0 to 30% hyaluronic acid.

26. A cosmetic method for inducing the synthesis of hyaluronic acid, wherein a cosmetic product combination comprising a first cosmetic composition comprising from 0.1% to 10% by weight of glucosamine sulfate, a second cosmetic composition comprising at least 4% by weight of hyaluronic acid and a third cosmetic composition comprising from 0.1% to 10% by weight of collagen, or a cosmetic composition comprising from 0.1% to 10% by weight of glucosamine sulfate, at least 4% by weight of hyaluronic acid hyaluronic acid and from 0.1% to 10% by weight of collagen is administered orally or topically or both to an individual in need thereof, the glucosamine sulfate, hyaluronic acid and collagen exhibit synergistic synthesis of hyaluronic acid by fibroblasts.

27. The cosmetic method according to claim 26, to combat the reappearance of wrinkles and facial sagging after injections of hyaluronic acid in the skin on the face and/or on all body parts that have been the subject of a restorative injection procedure, aesthetic or cosmetic subsequent or concurrent, but not exceeding six months between the 2 procedures.

28. A cosmetic method for limiting the appearance of fine lines, wrinkles and facial sagging by inducing the synthesis of hyaluronic acid, wherein a cosmetic product combination comprising a first cosmetic composition comprising from 0.1% to 10% by weight of glucosamine sulfate, a second cosmetic composition comprising at least 4% by weight of hyaluronic acid hyaluronic acid and a third cosmetic composition comprising from 0.1% to 10% weight of collagen, or a cosmetic composition comprising from 0.1% to 10% b weight of glucosamine sulfate, at least 4% by weight of hyaluronic acid and from 0.1% to 10% by weight of collagen is administered orally or topically or both to an individual in need thereof, wherein the glucosamine sulfate, hyaluronic acid and collagen exhibit synergistic synthesis of hyaluronic acid by fibroblasts.

29. A cosmetic method for extending the duration of efficacy of beauty treatments made using filler compositions (filler) by inducing the synthesis of hyaluronic acid, wherein a cosmetic product combination comprising a first cosmetic composition comprising from 0.1% to 10% by weight of glucosamine sulfate, a second cosmetic composition comprising at least 4% by weight of hyaluronic acid hyaluronic acid and a third cosmetic composition comprising from 0.1% to 10% by weight of collagen, or a cosmetic composition comprising from 0.1% to 10% by weight of glucosamine sulfate, at least 4% by weight of hyaluronic acid and from 0.1% to 10% by weight of collagen is administered orally or topically or both, to an individual after having a beauty treatment, wherein the glucosamine sulfate, hyaluronic acid and collagen exhibit synergistic synthesis of hyaluronic acid by fibroblasts.

Description

FIGURES

(1) FIG. 1: Synergistic effect of glucosamine sulfate and hyaluronic acid on the production of hyaluronic acid by NHEK cells (Normal Human Epidermal Keratinocytes): four series of results are shown (from left to right) which represent:

(2) 1.sup.st series: hyaluronic acid production by NHEK cells obtained by means of the combination of glucosamine sulfate and hyaluronic acid in the following proportions of glucosamine sulphate (μg/ml) Hyaluronic-acid (ng/ml) (left to right): 0.25 to 25 μg/ml-40 to 6000 ng/ml;

(3) 2.sup.nd series: hyaluronic acid production by NHEK cells obtained by hyaluronic acid alone in the proportion of hyaluronic acid (ng/ml) (from left to right): 40 to 6000 ng/ml;

(4) 3.sup.th series: hyaluronic acid production by NHEK cells obtained by means of one glucosamine sulfate in the proportion of glucosamine sulfate (ug/ml) (left to right): 0.25 to 25 μg/ml; et

(5) 4.sup.th series: RA control.

(6) FIG. 2: Synergistic effect of glucosamine sulfate, hyaluronic acid and collagen on the production of hyaluronic acid by NHDF cells (Normal Human Dermal Fibroblasts): six sets of results are shown (from left to right) representing:

(7) 1.sup.st series: hyaluronic acid production by NHDF cells obtained by means of one glucosamine sulfate in the proportion of glucosamine sulfate (ug/ml) (left to right): 0.1 to 100 μg/ml;

(8) 2.sup.nd series: hyaluronic acid production by NHDF cells obtained by hyaluronic acid alone in the proportion of hyaluronic acid (ng/ml) (from left to right): 40 to 6000 ng/ml;

(9) 3.sup.rd series: hyaluronic acid production by NHDF cells obtained by means of the combination of glucosamine sulfate and hyaluronic acid in the following proportions of glucosamine sulphate (μg/ml) and hyaluronic acid (ng/ml) (from left to right): 0.1 to 100 μg/ml and 40 to 6000 ng/ml;

(10) 4.sup.th series: hyaluronic acid production by NHDF cells obtained by means of the combination of hyaluronic acid source and collagen only in the following proportions (ng/ml): 4 to 4000 ng/ml;

(11) 5.sup.th series: hyaluronic acid production by NHDF cells obtained by means of the combination of glucosamine sulfate and a hyaluronic acid and collagen source in the following proportions of glucosamine sulphate (μg/ml) and source of hyaluronic acid and collagen (ng/ml): 0.1 to 100 μg/ml and 4 to 4000 ng/ml; and

(12) 6.sup.th series: TGF-beta control.

EXAMPLES

Example 1: Synergistic Effect of Glucosamine Sulfate and Hyaluronic Acid on the Production of Hyaluronic Acid by NHEK Cells

(13) Normal human epidermal keratinocytes were cultured at 37° C. under 5% CO.sub.2 in a medium keratinocyte-SFM culture, supplemented with epidermal growth factor (EGF; 0.25 ng/ml), a pituitary extract (25 μg/ml) and an antibiotic (gentamicin 25 μg/ml) in 96-well plates for 24 h without stimulation.

(14) Glucosamine and/or hyaluronic acid are then added to the cultivation medium alone or in combination at concentrations of 40 to 6000 ng/ml hyaluronic acid and 0.25 to 25 μg/ml to glucosamine sulfate. After 72 hours of cultivation under these conditions, the supernatant was collected to quantify the hyaluronic acid produced by the cells using an ELISA Duoset Hyaluronan kit marketed by the company R & D System (DY3614 reference).

(15) The results obtained and presented in FIG. 1 show an induction of hyaluronic acid production by human keratinocytes in vitro, obtained by means of only glucosamine sulfate.

(16) They also demonstrate that contact of keratinocytes with hyaluronic acid alone does not stimulate the synthesis of hyaluronic acid.

(17) However, a synergistic induction of hyaluronic acid production by human keratinocytes in vitro, is clearly obtained by combining glucosamine sulfate and hyaluronic acid.

(18) Indeed, the observed results allow to conclude here to a synergistic effect between glucosamine sulfate and hyaluronic acid in comparison with the results obtained with each of the compounds alone, with an increased effect of 246% (p<0.001).

Example 2: Synergistic Effect of Glucosamine Sulfate, Hyaluronic Acid and Collagen on the Synthesis of Hyaluronic Acid

(19) Normal human dermal fibroblasts (NHDF) were cultured at 37° C. under 5% CO.sub.2 in a DMEM culture medium supplemented with glutamine (2 mM); calf serum (1%), penicillin (50 U/ml) and streptomycin (50 mg/ml).

(20) Glucosamine (0.1 to 100 μg/ml) and/or hyaluronic acid (from 40 to 6000 ng/ml) and/or a source of collagen and hyaluronic acid (in a ratio of between 0.07% and 15%) at concentrations ranging from 4 to 4000 ng/ml were then added to the cultivation medium alone or in combination.

(21) After 72 hours of cultivation under these conditions, the supernatant was collected to quantify the hyaluronic acid produced by the cells using an ELISA Duoset Hyaluronan kit marketed by the company R & D System (DY3614 reference).

(22) The results obtained and presented in FIG. 2 show that the synergistic induction of hyaluronic acid production by human fibroblasts in vitro, is clearly obtained by combining glucosamine sulfate, hyaluronic acid and collagen.

(23) Indeed, the results observed allow to conclude here to a synergistic effect between glucosamine sulfate, hyaluronic acid and collagen compared to the lack of stimulation obtained by administering only glucosamine sulfate or natural cartilage extract alone comprising hyaluronic acid and collagen or the combination only hyaluronic acid and glucosamine.