Hand Sanitizing Composition

Abstract

A hand sanitizing composition including one or more alcohols, a solubilizer, water, and unsaponifiable oil fractions of fatty triglyceride oils. The unsaponifiable oil fractions of fatty triglyceride oils may include a combination of any of the following: squalane, squalene, glycolipids, and phytosterols.

Claims

1. A hand sanitizing composition comprising one or more alcohols, a solubilizer, water, and a plant cell oil obtained from the unsaponifiable oil fractions of fatty triglyceride oils; wherein the hand sanitizing composition is free of emulsifiers, and wherein the solubilizer is a 3-10 carbon containing diol or triol.

2. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions of fatty triglyceride oils comprise squalane.

3. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions of fatty triglyceride oils comprise squalene.

4. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions of fatty triglyceride oils comprise glycolipids.

5. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions of fatty triglyceride oils comprise phytosterols.

6. A hand sanitizing composition according to claim 1, wherein the one or more alcohols comprise ethanol, or isopropanol or a mixture of ethanol and isopropanol.

7. A hand sanitizing composition according to claim 1, wherein the solubilizer comprises propandiol.

8. A hand sanitizing composition according to claim 1, wherein the one or more alcohols is present in an amount of 65-90 wt %.

9. A hand sanitizing composition according to claim 1, wherein the solubilizer is present in an amount of 0.5-3 wt %.

10. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions are present in amount of 0.25-2 wt %.

11. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions comprise 55-70 wt % squalane.

12. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions comprise 15-25 wt % squalene.

13. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions comprise 10-15 wt % glycolipids.

14. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions comprise 1-7 wt % phytosterol.

15. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions of fatty triglyceride oils comprise a combination of any of the following: squalane, squalene, glycolipids, and phytosterols.

16. A hand sanitizing composition according to claim 1, wherein the unsaponifiable oil fractions of fatty triglyceride oils comprise a combination of 55-70 wt % squalane, 15-25 wt % squalene, 10-15 wt % glycolipids and 17 wt % phytosterol.

Description

BRIEF DESCRIPTION OF THE FIGURES

[0052] FIG. 1 shows TEER measurements.

[0053] FIG. 2 shows moisturization levels of the skin after application of hand sanitizer.

EXAMPLES

[0054] The following examples illustrate embodiments of the present invention.

Formulation 1

[0055] 8 g alcohol (7.2 g ethanol and 0.8 g isopropanol)

[0056] 50 mg OLEACLEAR OLIVE SQUALANE

[0057] 2 g water

Formulation 2

[0058] 8 g alcohol (7.2 g ethanol and 0.8 g isopropanol)

[0059] 50 mg OLEACLEAR OLIVE SQUALANE

[0060] 200 mg ZEMEA

[0061] 1.75 g water

Formulation 3

[0062] 8 g alcohol (7.2 g ethanol and 0.8 g isopropanol)

[0063] 50 mg PLANELL

[0064] 200 mg ZEMEA

[0065] 1.75 g water

Formulation 4

[0066] 8 g alcohol (7.2 g ethanol and 0.8 g isopropanol)

[0067] 50 mg PLANELL

[0068] 100 mg ZEMEA

[0069] 1.85 g water

Formulation 5

[0070] 8 g alcohol (7.2 g ethanol and 0.8 g isopropanol)

[0071] 50 mg PLANELL

[0072] 200 mg Glycerine

[0073] 1.75 g water

Formulation 6

[0074] 8 g alcohol (7.2 g ethanol and 0.8 g isopropanol)

[0075] 50 mg PLANELL

[0076] 100 mg Glycerine

[0077] 1.85 g water

Formulation 7

[0078] 8 g alcohol (7.2 g ethanol and 0.8 g isopropanol)

[0079] 50 mg PLANELL

[0080] 100 mg isopentyl diol

[0081] 1.85 g water

[0082] Formula 4 is formulated as follows: [0083] 1. Add the PLANELL and the ZEMEA to the isopropanol with stirring until dissolved [0084] 2. Carefully add the ethanol to the mixture [0085] 3. Slowly add the water phase to the ethanol phase with stirring

[0086] For all formulas but Formula 4 the blending is done in the following: [0087] 1. Pre-mix ethanol and isopropanol [0088] 2. Add the other ingredients (except water) to the alcohol with stirring until dissolved [0089] 3. Slowly add the water phase to the ethanol phase with stirring

Example A

[0090] Example A discloses the results for testing the antibacterial effect of formulation 4 according to the method EN13727. The results show that the formulation according to the invention has a strong antibacterial effect.

Test Organism: Pseudomonas aeruginosa NCTC 12924 Lot B2942 Theoretical Concentration: 8.57.10.SUP.7 .CFU/Ml

[0091]

TABLE-US-00001 Real conc. of Na Contac time the product Dilution step V.sub.c1 V.sub.c2 (=x.sub.wm 10) Lg Na Lg R (min) 97 10.sup.0 0 0 <140 <2.15 >5.78 0.5 .sup.10.sup.1 0 0 <140 <2.15 >5.78 (30 s) 10.sup.0 0 0 <140 <2.15 >5.78 1 .sup.10.sup.1 0 0 <140 <2.15 >5.78

Test Organism: Staphylococcus aureus NCTC 10788 Lot B2834 Theoretical Concentration: 9.19.10.SUP.7 .CFU/Ml

[0092]

TABLE-US-00002 Real conc. of Na Contact time the product Dilution step V.sub.c1 V.sub.c2 (=x.sub.wm 10) Lg Na Lg R (min) 97 10.sup.0 0 0 <140 <2.15 >5.81 0.5 .sup.10.sup.1 0 0 <140 <2.15 >5.81 (30 s) 10.sup.0 0 0 <140 <2.15 >5.81 1 .sup.10.sup.1 0 0 <140 <2.15 >5.81

Test Organism: Escherichia coli NCTC 12923 Lot B2710 Theoretical Concentration: 8.87.10.SUP.7 .CFU/Ml

[0093]

TABLE-US-00003 Real conc. of Na Contact time the product Dilution step V.sub.c1 V.sub.c2 (=x.sub.wm 10) Lg Na Lg R (min) 97 10.sup.0 0 0 <140 <2.15 >5.8 0.5 .sup.10.sup.1 0 0 <140 <2.15 >5.8 (30 s) 10.sup.0 0 0 <140 <2.15 >5.8 1 .sup.10.sup.1 0 0 <140 <2.15 >5.8

Example B

[0094] Example B shows TEER Measurements (trans-epithelial-electrical-resistance).

[0095] TEER measurements shows the movement of ions across the paralcellular pathway regulated by polarized plasma membrane surfaces and by cell-to-cell tight junctions that together prevent movement of solutes and ions across the epithelia. TEER is an indirect assessment of tight junction stability and consequently is a direct measure of the functionality of barrier function in epithelial tissue: it reflects the global resistance of the barrier linked both to the structure and to epithelial thickness. Maintenance of stability and electrical resistance of an epithelium is critical for essential physiological processes, therefore significant changes in TEER may represent an early expression of cell damage.

[0096] FIG. 1 shows the result of TEER measurements on a negative control, ethanol, a formulation control consisting of 72 wt % ethanol and 8 wt % isopropanol, and on formulation 4. It clearly shows that formulation 4 has less damaging effect on skin than ethanol and the formulation control.

Example C

[0097] FIG. 2 shows moisturization level of skin of one test person. The test method involved repeated rapid application. The formulation was used to saturate medical gauze pads. The test was carried out by wiping the pad over the test area, allowing alcohol to evaporate and reapplying as soon as the area was dry. The formulation was applied 20 times in succession on day 1 and 15 times in succession on day 2. The condition of the skin was measured: before the application, 1 hour after completed application, at intervals in the following days up to the fourth day. Untreated control sites were used to follow the condition of the skin as this can be expected to vary over the trial. The moisture level on the skin was measured using a corneometer (middle value of three consecutive measurements). Test formulation was applied to upper left forearm (other formulations were tested on lower left and upper and lower right forearm) Control sites were located mid forearm on both right and left arm.