POLYURETHANE-HYDROGEL COMPOSITION COMPRISING CHLORHEXIDINE

Abstract

The invention is directed to a composition comprising: a polyurethane substrate; a hydrogel forming polymer on at least one of the two faces of the polyurethane substrate; and chlorhexidine on the at least one face comprising the hydrogel forming polymer; wherein the hydrogel polymer is covalently linked to the polyurethane substrate. The invention is also directed to a dressing in comprising the composition and to a process for the preparation of the composition, the process comprising the following steps: a) in impregnating at least one face of a polyurethane substrate with a solution comprising a reactive diolefin and a free radical initiator; b) extracting the polyurethane from the solution and, after drying, immerging the polyurethane in a solution containing the initiator catalyst and the hydrogel forming polymer to obtain PU covalently bond to a hydrogel forming polymer; c) extracting the polyurethane-hydrogel composition and drying in air; d) immerging the product of step iii) into an aqueous solution of chlorhexidine; e) collecting the polyurethane-hydrogel-chlorhexidine product from the solution and drying the obtained composition in air.

Claims

1. Composition comprising: a. a polyurethane substrate; b. a hydrogel forming polymer on at least one of the two faces of the polyurethane substrate; c. chlorhexidine on the at least one face comprising the hydrogel forming polymer; wherein the hydrogel polymer is covalently linked to the polyurethane substrate.

2. Composition according to claim 1 wherein chlorhexidine present in an amount comprised between 0.01 and 10.0 wt % based on the total weight of the composition.

3. Composition according to claim 1 further comprising an antibacterial compound selected from Ag nanoparticles, polyhexamethylene biguanide (PHMB), quaternary ammonium derivatives such as benzalkonium chloride, cetrimide, benzethonium chloride, cetylpyridinium chloride, quinolones compounds such as dequalinium chloride, hydroxyquino line hydrochloride.

4. Composition according to claim 1 wherein the hydrogel forming polymer is polyvinylpyrrolidone.

5. Composition according to claim 1 wherein the hydrogel is present in an amount comprised between 1 and 50 wt % based on the amount of polyurethane plus hydrogel.

6. Composition according to claim 1 wherein the composition has an optical transmittance higher than 30%.

7. A dressing comprising: a composition comprising: a. a polyurethane substrate; b. a hydrogel forming polymer on at least one of the two faces of the polyurethane substrate; c. chlorhexidine on the at least one face comprising the hydrogel forming polymer; wherein the hydrogel polymer is covalently linked to the polyurethane substrate.

8. Process for the preparation of a composition, the process comprising the following steps: i) impregnating at least one face of a polyurethane substrate with a solution comprising a reactive diolefin and a free radical initiator; ii) extracting the polyurethane from the solution and, after drying, immerging the polyurethane in a solution containing the initiator catalyst and the hydrogel forming polymer to obtain PU covalently bond to a hydrogel forming polymer; iii) extracting the polyurethane-hydrogel composition and drying in air; iv) immerging the product of step iii) into an aqueous solution of chlorhexidine; v) collecting the polyurethane-hydrogel-chlorhexidine product from the solution and drying the obtained composition in air.

9. Process according to claim 8 further comprising the step: vi) depositing silver salts by immerging the polyurethane-hydrogel-chlorhexidine in a solution of the silver salt, followed by reduction by chemical or physical methods; and vii) collecting the product of step v) and drying it in air.

10. Process according to claim 9 wherein the physical methods comprise UV irradiation with low pressure quicksilver lamps, and the chemical methods comprise treatment an aqueous solution of ascorbic acid.

Description

[0025] The volume average particle size of the silver nanoparticles is preferably comprised in the range from 20 nm to 500 nm, more preferably between 50 and 400 nm, even more preferably the volume average particle size of the silver nanoparticles is smaller than 300 nm, e.g. from 20 to 300 nm, or from 20 to 250 nm, or from 20 nm to 200 nm, or from 20 nm to 150 nm, as measured with conventional method for nanoparticles determination, e.g. by dynamic light scattering with a Malvern instrument.

[0026] FIG. 1 shows the NMR spectrum of polyurethane used in the tests.

[0027] FIG. 2 shows the NMR spectrum of PVP.

[0028] FIG. 3 shows the NMR spectrum after formation of the hydrogel on the polyurethane sample. It is clear that most signals correspond to the signals of PVP, indicating that the surface of PU is uniformly covered by the PVP hydrogel.

[0029] FIG. 4 shows the UV-Visible spectrum of PU covered with hydrogel. The very low absorbance between 300 and 600 nm indicates a very good optical transparency of the PU-hydrogel support.

[0030] FIG. 5 shows the UV-Visible spectrum of a PU-Hydrogel-Chlorexidine sample.

[0031] FIG. 6 shows the UV-Visible spectrum of a PU-Hydrogel-Chlorexidine-Ag sample.

[0032] The dressing obtainable by the composition according to the invention can be used on skin wounds or at the entry port of a catheter implanted in a patient. The transparency of the dressing helps the visual check of the conditions of the skin underneath the dressing without removing the dressing itself.

[0033] Transparency of the dressing can be measured as optical transmittance according to spectroscopic UV-visible/Near IR analysis by using integrating spheres, which measure either the diffuse transmittance or reflectance of a liquid, solid or powder sample.

[0034] Typically, UV-Vis or UV-Vis/NIR spectrophotometers are used to measure the transmittance of a homogeneous, transparent liquid or solid sample. However, when a suspended liquid, opaque solid or powder is measured, the light incident to the sample is scattered and only a small portion of the diffusely transmitted or reflected light reaches the detector. An integrating sphere can acquire most of the light from the sample providing a more representative measurement of the sample.

[0035] The optical transmittance of the dressing according to the invention is preferably higher than 30%, more preferably higher than 50%, even more preferably higher than 60%.

EXPERIMENTAL

[0036] IR has been performed with the Alpha Bruker FT-IR spectrometer.

[0037] Ag has been measured by ICP-OES analysis with a Perkin Elmer Optima 8300.

[0038] UV-Vis/NIR spectrometry has been performed with a Jasco V-700 Series UV-Visible/NIR spectrophotometer equipped with a 60 mm UV-Visible/NIR Integrating Spheres.

[0039] The following reagents were used: Chlorexidine, 20% w/v non-sterile solution from Alfa Aesar; ascorbic acid from Sigma Aldrich reagent grade; sodium dodecyl sulfate from Sigma Aldrich, purity >98%; PVP from Sigma Aldrich, with average Mw of 360.000 Da.

Preparation of a PU Modified with a Gel-Forming Polymer

[0040] A polyurethane coated with PVP was prepared according to the method described by B. Butruk et al. Fabrication of biocompatible hydrogel coatings for implantable medical devices using Fenton-type reaction Material Science and Engineering C32 (2012) 1601-1609, whose content is herein incorporated by reference. A sample of polyurethane (PU) medical grade was immersed in a toluene solution containing EGDMA (10% by volume) and cumene hydroperoxide (CPH, 8% by volume). The sample was left in the solution for 5 minutes without stirring, to allow CPH and EGDMA to diffuse into the PU matrix. The sample was then extracted from the solution and dried in air for about 1 min and then immersed in an aqueous solution containing FeCl.sub.2x4H.sub.2O (0.1% w/v), ascorbic acid (AA, 1% w/v), and PVP 360 (5% w/v) for 15 minutes, stirring every 3-4 minutes manually. The sample was then washed abundantly and under magnetic stirring with the following solutions: aqueous solution of sodium dodecyl sulfate (0.1% w/v), a PBS (phosphate buffer saline) solution, a solution H.sub.2O/ethanol 60/40 (v/v), 2 washings with deionized water.

[0041] After the washings, the sample (PUH1) was left to dry in air, till a constant weight is reached (approx. 12 hours). The samples were weighted and by difference between the initial weight (0.5350 g) and the final weight (0.6094 g) it was calculated that the hydrogel was present on the PU in an amount of 0.0744 g, i.e. 14 wt % with respect to PU.

[0042] The procedure was repeated on a smaller PU sample (0.2568 g) leading to a PU-hydrogel sample (PUH2) of 0.2982 g containing an amount of hydrogel of 0.0414 g, equal to 16 wt % with respect to PU.

Deposition of Chlorhexidine on PU-Hydrogel

[0043] Two samples of PUH1 and PUH2 were charged with chlorhexidine by immerging the samples in a 5 wt % solution of chlorhexidine digluconate. After drying, the sample contained 4.8 wt % (PHU1) and 12.1 wt % (PHU2) of chlorhexidine.

[0044] However, part of chlorhexidine is not fixed onto the substrate in a stable way. In fact, when immerging the dried composition in water, it was noticed that a considerable amount of chlorhexidine is dissolved in water and the amount left on PU-hydrogel is respectively 2.74 wt % and 2.36 wt %. Table 1 shows the kinetic of release of chlorhexidine from PUH1. The kinetic was followed by UV analysis and the amount released measured by gravimetric analysis.

TABLE-US-00001 TABLE 1 Kinetic of release of chlorexidine Time (min) Abs (254 nm) mg released 0 0 5 0.3839 10 0.5257 10.98 20 0.4735 30 0.4628 45 0.4414 60 0.4413 120 0.4205 180 0.4210 900 0.4112 1500 0.3994 5760 0.4195 7200 0.4160 8.57 8640 0.3903

[0045] As it is clear from table 1, there is equilibrium between chlorhexidine dissolved and adsorbed in the PU-hydrogel and the amount measured in solution does not considerably change in time even when considering a period of 5 days.

Deposition of Ag Nanoparticles on PU-Hydrogel-Chlorexidine

[0046] Samples of about 200 mg PU-hydrogel-chlorexidine (30 wt % chlorexidine) were immersed in 100 ml, solution of 0.05 M AgNO.sub.3 for 24 hours. The Ag salt deposited on the sample was fixed onto it by reduction to metallic silver by 30 irradiation with low pressure quicksilver lamp. Large part of chlorexidine was recovered in the AgNO.sub.3 solution. (Ag=0.485 mg/100 mg or 2.19 mg/100 cm.sup.2; chlorexidine 0.155-0.21 mg/100 mg or 0.7-0.95 mg/100 cm.sup.2)

Bacteriostatic Activity of the PU-Hydrogel-Ag-Chlorexidine Dressing

[0047] The bacteriostatic activity of the dressing comprising chlorhexidine and Ag nanoparticles was tested according ASTM E 2180-01. Samples of the PU-Hydrogel-chlorexidine-Ag-dressing having an area of the surface of 9 cm.sup.2 where prepared in triplicate for each test. A thin layer of agar, previously contaminated, was distributed on each sample. Immediately after contamination and after defined contact times (24 h, 48 h, 4 days and 7 days) the microorganisms were collected by using a neutralizing nutrient solution. The various collected solutions were serially diluted, sown in double and incubated for 48/72 h at 37 C. Results are expressed as geometric mean and as percentage of reduction of the microorganisms with respect to the starting amount. The results indicate a bacteriostatic activity of 100% with respect to all strains tested.

TABLE-US-00002 Staphylococcus Staphylococcus Candida Pseudomonas epidermidis aereus albicans aeruginosa Starting inoculus 4.3 10.sup.7 2.6 10.sup.7 4.3 10.sup.7 8.0 10.sup.7 Ufc/g Ufc/g Ufc/g Ufc/g T 24 h <1 <1 <1 <1 T 48 h <1 <1 <1 <1 T 4 days <1 <1 <1 <1 T 7 days <1 <1 <1 <1