SALT FORM AND CRYSTAL FORM OF PYRAZOLE SUBSTITUTED IMIDAZO[1,2- A]QUINOXALINE DERIVATIVE

Abstract

Provided are a salt form and a crystal form of a pyrazole substituted imidazo[1,2-a]quinoxaline derivative, and a preparation method therefor. Specifically disclosed are a salt form and a crystal form of a compound of formula (I), and a preparation method therefor and the use thereof in the preparation of drugs related to dual inhibitors of spleen tyrosine kinase (Syk) and vascular endothelial growth factor 2 (VEGFR2).

##STR00001##

Claims

1. A crystal form A of a compound of formula (I), wherein an X-ray powder diffraction pattern of the crystal form A under Cu K? radiation has characteristic diffraction peaks at 2? angles of 11.96?0.20?, 14.14?0.20?, 16.76?0.20?, 17.55?0.20?, and 21.84?0.20?, ##STR00029##

2. The crystal form A according to claim 1, wherein an X-ray powder diffraction pattern of the crystal form A under Cu K? radiation has characteristic diffraction peaks at 2? angles of 11.96?0.20?, 14.14?0.20?, 15.36?0.20?, 16.76?0.20?, 17.55?0.20?, 21.84?0.20?, 23.49?0.20?, and 24.42?0.20?.

3. (canceled)

4. The crystal form A according to claim 1, wherein an XRPD pattern of the crystal form A is shown in FIG. 1.

5. The crystal form A according to claim 1, wherein a differential scanning calorimetry curve of the crystal form A has an endothermic peak at 332.4? C.?3? C.

6. The crystal form A according to claim 5, wherein a DSC pattern of the crystal form A is shown in FIG. 2.

7. The crystal form A according to claim 1, wherein a thermal gravimetric analysis curve of the crystal form A has a weight loss of 2.41?0.20% at 250.0? C.?3? C.

8. The crystal form A according to claim 7, wherein a TGA pattern of the crystal form A is shown in FIG. 3.

9. A crystal form B of a compound of formula (I), wherein an X-ray powder diffraction pattern of the crystal form B under Cu K? radiation has characteristic diffraction peaks at 2? angles of 12.14?0.20?, 19.52?0.20?, 22.08?0.20?, and 28.22?0.20?, ##STR00030##

10.-11. (canceled)

12. The crystal form B according to claim 9, wherein an XRPD pattern of the crystal form B is shown in FIG. 4.

13. The crystal form B according to claim 9, wherein a differential scanning calorimetry curve of the crystal form B has an endothermic peak at 331.8? C.?3? C.

14. The crystal form B according to claim 13, wherein a DSC pattern of the crystal form B is shown in FIG. 5.

15. The crystal form B according to claim 9, wherein a thermal gravimetric analysis curve of the crystal form B has a weight loss of 4.11?0.20% at 300.0? C.?3? C.

16. The crystal form B according to claim 15, wherein a TGA pattern of the crystal form B is shown in FIG. 6.

17. The crystal form B according to claim 9, wherein a preparation method of the crystal form B comprises: (a) dissolving a crystal form A of the compound of formula (I) in dimethylacetamide wherein an X-ray powder diffraction pattern of the crystal form A under Cu K? radiation has characteristic diffraction peaks at 2? angles of 11.96?0.20?, 14.14?0.20?, 16.76?0.20?, 17.55?0.20?, and 21.84?0.20?; (b) placing an opened glass vial containing a solution of the compound in a glass bottle filled with acetone in advance; (c) sealing the glass bottle and allowing gas and liquid permeation at 20? C. to 30? C. for three days; (d) removing a supernatant with a straw, and drying a remaining solid at room temperature in the bottle which is opened for five days to obtain the solid; (e) adding the solid into an aluminum crucible with a cover and slowly heating the solid to 150? C. under an atmosphere of dry nitrogen; and (f) cooling the solid down to 20? C. to 30? C.

18. A pharmaceutically acceptable salt of a compound of formula (I), wherein the pharmaceutically acceptable salt is a hydrate, maleate or gentisate, ##STR00031##

19. The pharmaceutically acceptable salt according to claim 18, wherein the pharmaceutically acceptable salt is a hydrate, maleate or gentisate, and has a structure represented by formula (II), formula (III) or formula (IV), ##STR00032## wherein: m is selected from 3.0, 3.5, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, and 5.0; n is selected from 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, and 1.3; and p is selected from 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, and 1.3.

20. (canceled)

21. A crystal form C of a compound of formula (II-1), wherein an X-ray powder diffraction pattern of the crystal form C under Cu K? radiation has characteristic diffraction peaks at 2? angles of 6.53?0.20?, 12.05?0.20?, and 13.05?0.20?, ##STR00033##

22.-23. (canceled)

24. The crystal form C according to claim 21, wherein an XRPD pattern of the crystal form C is shown in FIG. 7.

25. (canceled)

26. The crystal form C according to claim 21, wherein a DSC pattern of the crystal form C is shown in FIG. 8.

27.-35. (canceled)

36. A method of inhibiting the activity of spleen tyrosine kinase (Syk) and vascular endothelial growth factor 2 (VEGFR2) using the crystal form A according to claim 1.

37. A method of inhibiting the activity of spleen tyrosine kinase (Syk) and vascular endothelial growth factor 2 (VEGFR2) using the crystal form B according to claim 9.

38. A method of inhibiting the activity of spleen tyrosine kinase (Syk) and vascular endothelial growth factor 2 (VEGFR2) using the pharmaceutically acceptable salt according to claim 18.

39. A method of inhibiting the activity of spleen tyrosine kinase (Syk) and vascular endothelial growth factor 2 (VEGFR2) using the crystal form C according to claim 21.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0143] FIG. 1 is an XRPD pattern of a crystal form A of a compound of formula (I);

[0144] FIG. 2 is a DSC pattern of a crystal form A of a compound of formula (I);

[0145] FIG. 3 is a TGA pattern of a crystal form A of a compound of formula (I);

[0146] FIG. 4 is an XRPD pattern of a crystal form B of a compound of formula (I);

[0147] FIG. 5 is a DSC pattern of a crystal form B of a compound of formula (I);

[0148] FIG. 6 is a TGA pattern of a crystal form B of a compound of formula (I);

[0149] FIG. 7 is an XRPD pattern of a crystal form C of a compound of formula (II-1);

[0150] FIG. 8 is a DSC pattern of a crystal form C of a compound of formula (II-1);

[0151] FIG. 9 is an XRPD pattern of a crystal form D of a compound of formula (III-1);

[0152] FIG. 10 is a DSC pattern of a crystal form D of a compound of formula (III-1);

[0153] FIG. 11 is a TGA pattern of a crystal form D of a compound of formula (III-1);

[0154] FIG. 12 is an 1H NMR pattern of a crystal form D of a compound of formula (III-1);

[0155] FIG. 13 is an XRPD pattern of a crystal form E of a compound of formula (IV-1);

[0156] FIG. 14 is a DSC pattern of a crystal form E of a compound of formula (IV-1);

[0157] FIG. 15 is a TGA pattern of a crystal form E of a compound of formula (IV-1);

[0158] FIG. 16 is an 1H NMR pattern of a crystal form E of a compound of formula (IV-1);

[0159] FIG. 17 is an XRPD pattern of a crystal form F of a compound of formula (V-1);

[0160] FIG. 18 is an XRPD pattern of a crystal form G of a compound of formula (V-2);

[0161] FIG. 19 is an XRPD pattern of a crystal form H of a compound of formula (VI-1);

[0162] FIG. 20 is an XRPD pattern of a crystal form I of a compound of formula (VII-1);

[0163] FIG. 21 is an XRPD pattern of a crystal form J of a compound of formula (VII-2);

[0164] FIG. 22 is an XRPD pattern of a crystal form K of a compound of formula (VIII-1);

[0165] FIG. 23 is an XRPD pattern of a crystal form L of a compound of formula (VIII-1);

[0166] FIG. 24 is an XRPD pattern of a crystal form M of a compound of formula (IX-1); and

[0167] FIG. 25 is an XRPD pattern of a crystal form N of a compound of formula (IX-2).

DETAILED DESCRIPTION

[0168] The following examples are used to describe the present disclosure in detail, but do not imply any unfavorable limitation on the present disclosure. The present disclosure is described in detail herein, and specific embodiments are also disclosed. Various changes and improvements made to the specific embodiments of the present disclosure without departing from the spirit and scope of the present disclosure are obvious to persons skilled in the art.

PREPARATION EXAMPLES

Example 1 Preparation of Crystal Form A of Compound of Formula (I)

[0169] ##STR00014##

##STR00015##

Step 1

[0170] 16 L of dimethyl sulfoxide was first added to a reactor with a volume of 50 L. Mechanical stirring was started with a rotation speed of 150 rpm at an internal temperature shown as 15? C. Then 1950 g of compound 1a, 1230 g of 1H-imidazole-2-carboxylate methyl ester and 1880 g of sodium carbonate were added in sequence, heating was started, and an external heating temperature was set to 82? C. After stirring at an internal temperature of 78? C.-80? C. for 16 hours, the heating was ended, and the reaction solution was cooled to 30? C. The reaction solution was slowly added into 30 L of water while being stirred, and a large amount of yellow solid was precipitated. The external temperature was set to 10? C. and a resulting mixture was stirred for 50 minutes at 100 rpm. Filtration was started at an internal temperature shown as 15? C. to obtain a filtered solid which was vacuum-dried to obtain compound 1b.

[0171] .sup.1NMR (400 MHz, DMSO-d.sub.6) ? ppm 8.12 (d, J=8.5 Hz, 1H), 8.04 (d, J=J=2.0 Hz, 1H), 7.98 -7.93 (m, 1H), 7.45 (s, 1H), 7.09 (s, 1H), 3.86 (s, 3H).

Step 2

[0172] 18 L of ethanol and 4.5 L of water were first added to a reactor with a volume of 50 L. Stirring was started with a rotation speed of 150 rpm at an internal temperature shown as 15? C. Then 1665 g of the compound 1b, 1656 g of thiourea dioxide and 859.5 g of sodium bicarbonate were added in sequence. Heating was started, an external heating temperature was set to 65? C., and gas was discharged when the internal temperature rose to 60? C.-65? C. The temperature was maintained for 1 hour, and when no gas was discharged any more, the external temperature was raised to 80? C. After stirring for 16 hours, the heating was ended, and the reaction solution was cooled to 25? C. The reaction solution was slowly added into 25 L of water in two batches separately while being stirred, and a large amount of solid was precipitated. The external temperature was set to 10? C., after 30 minutes of stirring, filtration was started at an internal temperature shown as 15? C. The precipitated solid was added back into the reactor, stirring was started, 30 L of water was added, the internal temperature was raised to 65? C. via heating, and after 16 hours of stirring, the reaction solution was filtered immediately to obtain a filter cake which was vacuum-dried to obtain compound 1c.

[0173] .sup.1H NMR (400 MHz, DMSO-d.sub.6) ? ppm 11.95 (s, 1H), 8.61 (d, J=1.0 Hz, 1H), 8.45 (d, J=2.0 Hz, 1H), 7.62-7.54 (m, 1H), 7.34-7.25 (m, 1H).

Step 3

[0174] After an exhaust gas absorption device was established, 13.5 L of 1,4-dioxane was added to a reactor with a volume of 50 L. Stirring was started with a rotation speed of 150 rpm at an internal temperature shown as 15? C. Heating was started, an external heating temperature was set to 40? C., and then 900 g of the compound 1c and 825.98 g of N,N-dimethylaniline were added in sequence. 2610 g of phosphorus oxychloride was added dropwise with a constant pressure dropping funnel, and the internal temperature was controlled between 35? C. and 40? C. After the addition of the phosphorus oxychloride, the reaction solution was stirred at an internal temperature of 90? C.-92? C. for 16 hours, then the heating was ended, and the reaction solution was cooled to 25? C. The reaction solution was slowly added into 15 L of water in batches while being stirred. The external temperature was set to 30? C., the reaction solution was stirred for 1 hour, and 4 M sodium hydroxide aqueous solution was slowly added at an internal temperature of 15? C.-20? C. to adjust pH to 7. After being heated to an internal temperature of 30? C., the reaction solution was stirred for 30 minutes again and filtered to obtain a filter cake which was vacuum-dried to obtain compound 1d.

[0175] .sup.1H NMR (400 MHz, DMSO-d.sub.6) ?=, 9.01 (s, 1H), 8.80 (d, J=2.0 Hz, 1H), 7.95 (d, J=8.8 Hz, 1H), 7.91 (s, 1H), 7.84 (dd, J=2.0, 8.8 Hz, 1H).

Step 4

[0176] 9 L of dimethyl sulfoxide was added to a reactor with a volume of 50 L at 20? C. Stirring was started at a rotation speed of 150 rpm. 620.24 g of 4-morpholine aniline, 1124.4 g of N, N-diisopropylethanamine and 820.00 g of the compound id were added sequentially. The reaction solution was stirred for 24 hours at an internal temperature of 95? C.-100? C. The heating was ended, and the reaction solution was cooled to 25? C. The reaction solution was slowly added into 30 L of water while being stirred. The external temperature was set to 10? C., the reaction solution was stirred for 0.5 hours and filtered to obtain a filter cake which was put into the reactor again, and 9 L of isopropanol was added into the reactor. The reaction solution was stirred for 1 hour at an internal temperature of 82? C. The reaction solution was filtered immediately to obtain a filter cake which was washed with 2 L of hot isopropanol, and vacuum-dried at 40? C.-50? C. for 12 hours to obtain compound 1e.

[0177] .sup.1H NMR (400 MHz, DMSO-d.sub.6) ? ppm 9.65 (s, 1H), 8.74 (d, J=1.0 Hz, 1H), 8.48 (s, 1H), 7.99 (d, J=9.0 Hz, 2H), 7.59-7.55 (m, 2H), 7.08-6.91 (m, 3H), 3.78-3.72 (m, 4H), 3.10-3.05 (m, 4H).

Step 5

[0178] 4 L of dimethyltetrahydrofuran was added to a reactor with a volume of 50 L. Stirring was started at a rotation speed of 150 rpm. An external temperature was set to 5? C. 500.00 g of 4-pyrazoleboronic acid pinacol ester, 333.00 g of N,N-diisopropylethanamine were added sequentially. 558.00 g of (2-(chloromethoxy)ethyl)trimethylsilane was added dropwise with a constant pressure dropping funnel at internal temperature of 5? C.-8? C. After the addition of the (2-(chloromethoxy)ethyl)trimethylsilane, the external temperature was set to 20? C., and the reaction solution was stirred for 3 hours. Then 3.2 L of dimethyltetrahydrofuran, 1.8 L of water, 880.00 g of compound 1e, 640.00 g of potassium carbonate and 85.00 g of 1,1-bis(diphenylphosphino)ferrocene-dichloropalladium were further added to the reactor. The external temperature was set to 85? C. after nitrogen was injected into the reactor for displacement for 20 minutes. The reaction solution was stirred for 16 hours at an internal temperature of 80? C.-82? C. After the reaction solution was cooled to an internal temperature of 20? C., 2 L of water and 15 L of n-heptane were added into the reactor while being stirred. After being stirred for 20 minutes, the reaction solution was filtered to obtain a filter cake which was dried to obtain 1200 g of crude compound 1f. Then the crude compound 1f was added into a reactor with a volume of 50 L and 12 L of dimethyltetrahydrofuran was added. Heating was started and the external temperature was set to 80? C. After the solid was dissolved, 360.00 g of activated carbon was added. The reaction solution was stirred for 16 hour at an internal temperature of 80? C. The reaction solution was filtered to obtain a filtrate which was cooled to 15? C.-20? C., and 20 L of n-heptane was added. The reaction solution was stirred at 15? C.-20? C. for 16 hours and filtered to obtain a filter cake which was vacuum-dried to obtain compound 1f.

Step 6

[0179] 7 L of 1 M tetrabutylammonium fluoride solution in tetrahydrofuran was added to a reactor with a volume of 50 L, and stirring was started at a rotation speed of 150 rpm. 700.00 g of the compound 1f and 77.69 g of ethylenediamine were added sequentially. An external temperature was set to 72? C., and the reaction solution was stirred for 16 hours at an internal temperature of 67? C. 5.6 L of tetrahydrofuran was further added into the reactor, and the reaction solution was stirred at an internal temperature of 40? C. for 2 hours, and then the reaction solution was stirred at 30? C. for 2 hours, and then stirred at 20? C. for 16 hours. The reaction solution was filtered to obtain a filter cake which was added back into the reactor, and 10 L of water and 5 L of ethanol were added and the mixture was stirred at an internal temperature of 60? C. for 16 hours. The reaction solution was filtered immediately to obtain a filter cake which was vacuum-dried to a constant weight to obtain the crystal form A of the compound of formula (I).

[0180] .sup.1H NMR (400 MHz, DMSO-d.sub.6) ?: 8.81 (s, 1H), 8.43 (s, 1H), 8.23 (s, 2H), 7.92 (d, J=8.8 Hz, 2H), 7.80 (s, 1H), 7.77-7.72 (m, 1H), 7.71-7.63 (m, 1H), 7.07 (d, J=8.8 Hz, 2H), 3.79-3.76 (m, 4H), 3.16 (br. s., 4H); MS (ESI) m/z: 412 [M+H].sup.+.

Example 2 Preparation of Crystal Form B of Compound of Formula (I)

[0181] ##STR00016##

[0182] 19.1 mg of the solid crystal form A of the compound of formula (I) was weighed and added into a 3.0 mL glass vial, and 0.5 mL of dimethylacetamide was added to dissolve the solid to obtain a clear solution. A 3.0 mL opened glass vial containing the clear solution was placed in a 20 mL glass bottle filled with 3 mL of acetone in advance. After being sealed, the glass bottle was placed at room temperature for three days for gas and liquid penetration, a supernatant was removed with a straw, and a remaining solid was dried at room temperature in the glass bottle which was opened for five days to obtain the solid. Then an appropriate amount of solid was weighed and added into an aluminum crucible with a cover, and the solid was heated from room temperature to 150? C. at a heating rate of 10? C./min under protection of dry nitrogen injected at 50 mL/min, and then cooled down to room temperature. The crystal form B of the compound of formula (I) was obtained via XRPD detection.

Example 3 Preparation of Crystal Form C of Compound of Formula (II-1)

[0183] ##STR00017##

[0184] 20.1 mg of the solid crystal form A of the compound of formula (I) was weighed and added into a glass vial, 3.0 mL of tetrahydrofuran/water (volume ratio: 4:1) was added to dissolve the solid, and a sample solution was filtered with a polytetrafluoroethylene filter membrane with a 0.45 micron pore size into a new 3.0 mL glass vial to obtain a clear solution which was slowly evaporated at room temperature for three days to obtain a solid when the solvent was removed by evaporation. The crystal form C of the compound of formula (II-1) was obtained via XRPD detection.

Example 4 Preparation of Crystal Form D of Compound of Formula (III-1)

[0185] ##STR00018##

[0186] 20.4 mg of the solid crystal form A of the compound of formula (I) was weighed and added into a glass vial, 0.5 mL of ethanol/dimethyl sulfoxide (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 5.7 mg of maleic acid was added, to obtain a milky white suspension. The milky white suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form D of the compound of formula (III-1) was obtained via XRPD detection.

Example 5 Preparation of Crystal Form E of Compound of Formula (IV-1)

[0187] ##STR00019##

[0188] 18.4 mg of the solid crystal form A of the compound of formula (I) was weighed and added into a glass vial, 0.5 mL of ethanol/dimethyl sulfoxide (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 7.5 mg of gentisic acid was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form E of the compound of formula (IV-1) was obtained via XRPD detection.

Example 6 Preparation of Crystal Form F of Compound of Formula (V-1)

[0189] ##STR00020##

[0190] 20.8 mg of the solid crystal form A of the compound of formula (I) was weighed and added into a glass vial, 0.5 mL of ethanol/dimethyl sulfoxide (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 4.9 ?L of concentrated hydrochloric acid was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form F of the compound of formula (V-1) was obtained via XRPD detection.

Example 7 Preparation of Crystal Form G of Compound of Formula (V-2)

[0191] ##STR00021##

[0192] 19.3 mg of the solid crystal form A of the compound of formula (I) was weighed and added into an HPLC glass vial, 0.5 mL of tetrahydrofuran/water (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 4.9 ?L of hydrochloric acid was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form G of the compound of formula (V-2) was obtained via XRPD detection.

Example 8 Preparation of Crystal Form H of Compound of Formula (VI-1)

[0193] ##STR00022##

[0194] 19.2 mg of the solid crystal form A of the compound of formula (I) was weighed and added into an HPLC glass vial, 0.5 mL of ethanol/dimethyl sulfoxide (volume ratio: 19:1) was added, and a reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 12.2 ?L of sulfuric acid was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form H of the compound of formula (VI-1) was obtained via XRPD detection.

Example 9 Preparation of Crystal Form I of Compound of Formula (VII-1)

[0195] ##STR00023##

[0196] 22.5 mg of the solid crystal form A of the compound of formula (I) was weighed and added into an HPLC glass vial, 0.5 mL of ethanol/dimethyl sulfoxide (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 2.6 ?L of phosphoric acid was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form I of the compound of formula (VII-1) was obtained via XRPD detection.

Example 10 Preparation of Crystal Form J of Compound of Formula (VII-2)

[0197] ##STR00024##

[0198] 18.5 mg of the solid crystal form A of the compound of formula (I) was weighed and added into an HPLC glass vial, 0.5 mL of tetrahydrofuran/water (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 2.6 ?L of phosphoric acid was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form J of the compound of formula (VII-2) was obtained via XRPD detection.

Example 11 Preparation of Crystal Form K of Compound of Formula (VIII-1)

[0199] ##STR00025##

[0200] 21.3 mg of the solid crystal form A of the compound of formula (I) was weighed and added into an HPLC glass vial, 0.5 mL of ethanol/dimethyl sulfoxide (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 8.5 mg of p-toluenesulfonic acid was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form K of the compound of formula (VIII-1) was obtained via XRPD detection.

Example 12 Preparation of Crystal Form L of Compound of Formula (VIII-1)

[0201] ##STR00026##

[0202] 18.6 mg of the solid crystal form A of the compound of formula (I) was weighed and added into an HPLC glass vial, 0.5 mL of tetrahydrofuran/water (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 8.3 mg of p-toluenesulfonic acid was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form L of the compound of formula (VIII-1) was obtained via XRPD detection.

Example 13 Preparation of Crystal Form M of Compound of Formula (IX-1)

[0203] ##STR00027##

[0204] 18.6 mg of the solid crystal form A of the compound of formula (I) was weighed and added into an HPLC glass vial, 0.5 mL of ethanol/dimethyl sulfoxide (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 9.9 mg of 40% hydrobromic acid aqueous solution was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain the solid. The crystal form M of the compound of formula (IX-1) was obtained via XRPD detection.

Example 14 Preparation of Crystal Form N of Compound of Formula (IX-2)

[0205] ##STR00028##

[0206] 18.9 mg of the solid crystal form A of the compound of formula (I) was weighed and added into an HPLC glass vial, 0.5 mL of tetrahydrofuran/water (volume ratio: 19:1) was added, and the reaction solution was subjected to magnetic stirring at room temperature (at about 600 rpm), 9.8 mg of 40% hydrobromic acid aqueous solution was added, to obtain a yellow suspension. The yellow suspension was subjected to magnetic stirring at 50? C. (at about 700 rpm) for four days, and after centrifugation, a resulting solid was subjected to vacuum drying at room temperature for two hours to obtain a solid. The crystal form N of the compound of formula (IX-2) was obtained via XRPD detection.

Characterization Examples

Example 1: Solid Stability Test of Crystal Form A of Compound of Formula (I)

[0207] According to Guidelines for the Stability Testing of Drug Substances and Preparations (General Chapter 9001 in the Chinese Pharmacopoeia, Volume IV, 2015 Edition), in order to evaluate solid stability of the crystal form A of the compound of formula (I), the crystal form A was subjected to stability evaluation with respect to influencing factors (high temperature, high humidity and sunlight) and 60? C./75% RH and 40? C./75% RH conditions. The crystal form A was placed under conditions of high temperature (60? C., sealed) and high humidity (92.5% RH, wrapped in parafilm with 5 pierced small holes) for 1 week and 2 weeks, respectively. According to ICH conditions (total illuminance of visible light reached 1200000 lux.Math.hrs, and total ultraviolet light illuminance reached 200 W.Math.hrs/m.sup.2), the crystal form A was sealed and placed under visible light and ultraviolet light (a control group sample was placed in the dark at the same time and wrapped in tinfoil) under 60? C./75% RH (wrapped in parafilm pierced with 5 small holes) for 1 and 2 months, and placed under the condition of 40? C./75% RH (wrapped with parafilm with 5 pierced small holes) for 1, 2 and 3 months. All the stability samples were subjected to XRPD detection to detect changes in crystal forms. The results are shown in Table 19.

[0208] About 10 mg of the crystal form was accurately weighed, put in a dry and clean glass bottle, spread into a thin layer, covered with aluminum foil with small pierced holes, and put under conditions of the influencing factor test and accelerated conditions. A sample under sunlight conditions (1200000 Lux visible light, and 200 W ultraviolet light) was put in a transparent glass bottle and fully exposed, and a sample used for XRPD detection was put separately.

[0209] After the sample was taken out by a time point, the glass bottle was covered with a lid, sealed with parafilm, and stored in a refrigerator at ?20? C. During sample preparation, the sample was taken out of the refrigerator and recovered to room temperature, 10 mL of 80% ACN was added, and the sample was dissolved by ultrasonication for 2 minutes to obtain a solution at a concentration of about 1 mg/mL. A liquid phase was used for sample analysis, and the test result was compared with an initial test result on Day 0. The test results are shown in Table 19 below.

[0210] Preparation of a standard solution on Day 0: About 10 mg of the crystal form was weighed, put into a 10 mL volumetric flask, dissolved in 80% acetonitrile and diluted to the scale.

[0211] In addition, all the stability samples were subjected to the HPLC tests. Specific results are summarized in Table 19.

TABLE-US-00019 TABLE 19 Results of Solid Stability Test of a Crystal Form A of a Compound of Formula (I) Purity Test condition Sampling condition (area %) Crystal form Day 0 99.05 Crystal form A High temperature 1 week 98.99 Crystal form A (60? C., opened) 2 week 99.10 Crystal form A High humidity 1 week 99.03 Crystal form A (relative humidity 2 week 99.11 Crystal form A 92.5%, opened) Visible light + Total illuminance of visible 99.12 Crystal form A ultraviolet light light reached 1200000 lux .Math. hrs (ICH condition) Total ultraviolet light illuminance reached 300 W .Math. hrs/m.sup.2 Control group kept Under both visible light and 99.12 Crystal form A in the dark ultraviolet light, and wrapped with tin foil High temperature 1 month 99.15 Crystal form A and high humidity 2 months 99.03 Crystal form A (60? C., relative humidity 75%, opened) High temperature 1 months 99.12 Crystal form A and high humidity 2 months 99.06 Crystal form A (40? C., relative 3 months 99.04 Crystal form A humidity 75%, opened)

[0212] Conclusion: The crystal form A of the compound of formula (I) experienced no significant change in purity and crystal form under all stability conditions (high temperature, high humidity and sunlight), and had good chemical stability.

Activity Test

1. In Vitro Evaluation of Inhibitory Activity Against Syk Protein Kinase

[0213] Test purpose: to measure an inhibitory IC.sub.50 value of the compounds against Syk protein kinase.

[0214] Test material: Syk kinase (Invitrogen, PV3857) [0215] DTT (Sigma#43815): dithiothreitol [0216] ATP (Sigma#A7699): triphosadenine [0217] MgCl.sub.2 (Sigma#63020): magnesium chloride [0218] MnCl.sub.2 (Sigma#M1787): manganese chloride [0219] EDTA (Invitrogen#15575-020): ethylenediaminetetraacetic acid [0220] HEPES Buffer (Invitrogen#15630-080): zwitterionic sulfonic acid buffer [0221] HTRF? KinEASE? TK (Cisbio#62TK0PEC, 20000 tests): HTRF kinase kit [0222] Low volume, 384-well, white polystyrene plate (Greiner#784075) [0223] 384-well microplates (Greiner#781946) [0224] Centrifuge (Eppendorf#5810R) [0225] Micropipette (Eppendorf) [0226] Pipette (Greiner) [0227] Pipettor (Eppendorf) [0228] Mutidorp injector [0229] POD 810 Plate Assembler Fully Automatic Microplate Pretreatment System [0230] Envision Reader

Test Procedure and Method:

[0231] (a) Compound dilution and microplate injection [0232] 1) Compound powder was weighed and dissolved in a specific amount of DMSO at an initial concentration of 10 mM. [0233] 2) The compound was diluted to have a concentration of 0.74 mM, POD18 was used for microplate injection by 135 nL per well, an initial concentration of the compound was 10 ?M, and 11 concentrations and 3-fold descending serial dilution were carried out. [0234] (b) Reaction stage of enzyme and substrate [0235] 1) Preparation of test buffer dilution: 5?HTRF buffer in the kit was diluted to 1?HTRF buffer, and a specified amount of DTT and MgCl.sub.2 solution were added for later use. [0236] 2) A Syk enzyme reaction solution was prepared with 1?HTRF buffer, so that a final reaction concentration of Syk kinase was 0.0156 ng/?L. [0237] 3) A TK-Substrate-biotin/ATP mixture was prepared, so that a final substrate concentration was controlled to be 0.2 ?M. An ATP concentration was controlled to be 2 ?M. [0238] 4) A mutidorp injector was used to add the sample, 5 ?L of Syk enzyme solution and TK-Substrate-biotin/ATP mixture were added to each well, and incubated at 23? C. for 1 hour. [0239] (c) Test phase: [0240] 1) 13.33 mL of ethylenediaminetetraacetic acid solution was added to the test buffer of the kit, and specified amounts of Eu-labeled antibody and XL-665 were added, to prepare the test solution. [0241] 2) The sample was added with a mutidorp injector, and 10 ?L of the test solution was added to each well and incubated at 23? C. for 1 hour. A reaction of the mixture of enzyme and substrate was quenched. [0242] 3) Values on the Envision Reader were read after centrifugation. [0243] (d) Data analysis: XL-Fit was used to analyze data, and the IC.sub.50 value of the compound was calculated. The results are shown in Table 20.
Test results:

TABLE-US-00020 TABLE 20 Test Results of Inhibitory Activity Against Syk Compound IC.sub.50 (nM) against Syk Crystal form A of a compound of formula (I) 25

[0244] Conclusion: The crystal form A of the compound of formula (I) had good inhibitory activity against Syk.

2. In Vitro Evaluation of Inhibitory Activity Against KDR (VEGFR-2) Protein Kinase

[0245] Teat purpose

[0246] The KDR Kinase Kit was designed to use Kinase-Glo? MAX as a teat reagent to measure KDR kinase activity, and the IC.sub.50 value of the compound was used as an indicator for compound screening and analysis.

Test Method and Procedure

[0247] ADP-GLO? Kinase Assay is an assay reaction for measuring adenosine diphosphate formed by the kinase by using a luminescent kinase; and ADP was converted into ATP, that is, conversion into a light signal via Ultra-Glo? Luciferase. The light signal was positively correlated with the quantity of ADP and kinase activity. Such assay was very suitable for determining the activity of the compound, making the assay ideal for primary screening and a kinase selectivity assay. The ADP-Glo? Kinase Assay can be used to monitor the activity of almost all ADP-producing enzymes (for example, a kinase or ATPase): [0248] 1) Enzyme, substrate, ATP and inhibitors were diluted in kinase buffer; [0249] 2) 384-well plate: 1 ?L of inhibitor or (5% dimethyl sulfoxide solution) 2 ?L of KDR enzyme, and 2 ?L of substrate/ATP mixture; [0250] 3) The 384-well plate was incubated at room temperature for 60 minutes; [0251] 4) 5 ?L of ADP-GLO? reagent was added; [0252] 5) The 384-well plate was incubated at room temperature for 40 minutes; [0253] 6) 10 ?L of kinase test reagent was added; [0254] 7) The 384-well plate was incubated at room temperature for 30 minutes; [0255] 8) Lumination (integration time: 0.5 to 1 second) was recorded. Data was represented by a relative light unit (rlu), was directly correlated with an amount of ATP produced, and indicated correlation between a percentage of ATP converted into ADP for each kinase and a corresponding signal-to-background ratio. [0256] 9) Data analysis: (a) The KDR enzyme was titrated with 50 ?M of ATP to display a light signal indicating production of the KDR enzyme; and (b) 1.5 ng of KDR was used to generate staurosporine dose-response to determine the IC.sub.50 value of the inhibitor. The results are shown in Table 21 below.

Test Result

[0257]

TABLE-US-00021 TABLE 21 Test Results of Inhibitory Activity Against KDR Compound IC.sub.50 (nM) against KDR Crystal form A of a compound of formula (I) 28

[0258] Conclusion: The crystal form A of the compound of formula (I) had good inhibitory activity against KDR (VEGFR-2).

3. Pharmacokinetic Evaluation

[0259] Test purpose: to study pharmacokinetics of the compound in eye tissues of male SD rats after single administration of eye drop

[0260] Test material: SD rats (male, 7 to 10 weeks of age, WTLH/SLAC)

[0261] Test operation: In this test, 18 male SD rats were provided by Beijing Vital River Laboratory Animal Technology Co., Ltd., and were randomly divided into 6 groups (3 rats/group), and each group of animals were given 0.730 ?M (5 mg/mL for both eyes, 30 ?L/eye) of OT202 eye drops during single administration. All animals were not fasted before administration. Details about administration and blood sampling of the crystal form A of a compound of formula (I) are shown in Table 22 and Table 23 below, respectively.

TABLE-US-00022 TABLE 22 Parameters of Male SD Rats during Single Administration of Eye Drop Treatment Group Male/ Test Strength Dose Administration Route of Administration # Quantity preparation Batch No. (mg/mL) volume position administration frequency 01 3 OT202 20090102 5 30 ?L/ Both eyes Dripped into Once eye drop eye an eye 02 3 OT202 20090102 5 30 ?L/ Both eyes Dripped into Once eye drop eye an eye 03 3 OT202 20090102 5 30 ?L/ Both eyes Dripped into Once eye drop eye an eye 04 3 OT202 20090102 5 30 ?L/ Both eyes Dripped into Once eye drop eye an eye 05 3 OT202 20090102 5 30 ?L/ Both eyes Dripped into Once eye drop eye an eye 06 3 OT202 20090102 5 30 ?L/ Both eyes Dripped into Once eye drop eye an eye

TABLE-US-00023 TABLE 23 Blood Sampling Scheme After Single Administration of Eye Drop in Male SD Rats Animal Sampling time Group number Matrix point (h) 01 R01, R02 Plasma, bulbar conjunctiva, aqueous 0.5 and R03 humor of anterior chamber, cornea, and upper and lower eyelids 02 R04, R05 Plasma, bulbar conjunctiva, aqueous 1 and R06 humor of anterior chamber, cornea, and upper and lower eyelids 03 R07, R08 Plasma, bulbar conjunctiva, aqueous 3 and R09 humor of anterior chamber, cornea, and upper and lower eyelids 04 R10, R11 Plasma, bulbar conjunctiva, aqueous 6 and R12 humor of anterior chamber, cornea, and upper and lower eyelids 05 R13, R14 Plasma, bulbar conjunctiva, aqueous 12 and R15 humor of anterior chamber, cornea, and upper and lower eyelids 06 R16, R17 Plasma, bulbar conjunctiva, aqueous 24 and R18 humor of anterior chamber, cornea, and upper and lower eyelids

[0262] Relevant parameters of pharmacokinetics of the crystal form A of the compound of formula (I) in SD rats are shown in Table 24 below.

TABLE-US-00024 TABLE 24 Test Results of Pharmacokinetics Aqueous humor of Upper anterior Bulbar and lower Matrix Plasma chamber conjunctiva Cornea eyelids Sampling range (h) 1-6 6-24 1-24 1-24 1-24 for T.sub.1/2 calculation C.sub.max (nM) 1.23 560 4510 1590 10400 T.sub.max (h) 1.00 0.500 0.500 0.500 0.500 T.sub.1/2 (h) 4.80* 12.3 5.12* 4.84 9.45 T.sub.last (h) 6.00 24.0 24.0 24.0 24.0 AUC.sub.0-last (nM .Math. h) 5.32 657 15500 2530 65500 AUC.sub.0-inf (nM .Math. h) 9.56 711 16400 2590 80000 MRT.sub.0-last (h) 2.98 3.35 5.28 5.05 8.40 MRT.sub.0-inf (h) 7.39 6.27 6.73 5.74 13.7 AUC.sub.0-inf/AUC.sub.0-last (%) 180 108 106 102 122 AUC ratio 123 2910 476 12300 C.sub.max: maximum concentration; T.sub.max: time to peak concentration; T.sub.1/2: elimination half-life; T.sub.last: time to last measurable concentration; AUC.sub.0-last: area under the plasma concentration-time curve from time 0 to the last quantifiable time point; AUC.sub.0-inf: area under the plasma concentration-time curve from time 0 to infinity (extrapolated); MRT.sub.0-last: mean residence time from time 0 to the last quantifiable time point; MRT.sub.0-inf: mean residence time from time 0 to infinity (extrapolated); AUC ratio: tissue AUC.sub.0-last/plasma AUC.sub.0-last. *A linear regression coefficient of an elimination phase of a drug concentration was less than 0.9. : not applicable.

[0263] Conclusion: The crystal form A of the compound of formula (I) had good pharmacokinetic features, including a good eye-to-blood ratio, tissue exposure and so on.