14-CHLORO-BETA-ELEMENE NITRIC OXIDE DONOR TYPE DERIVATIVE, PREPARATION AND APPLICATION THEREOF

Abstract

The disclosure relates to the field of medicinal chemistry. Disclosed are a 14-chloro-?-elemene nitric oxide donor derivative and a preparation method and use thereof in the preparation of anti-tumor drugs. The 14-chloro-?-elemene nitric oxide donor derivative has a general formula shown in formula (I): in formula (I): R.sup.1 represents a linear or cyclic alcohol amine structure containing nitrogen and oxygen atoms; and each of R.sup.2 and R.sup.3 is independently selected from the group consisting of C.sub.1-10 alkyl, C.sub.3-12 cycloalkyl, C.sub.6-12 aryl, 5- to 10-membered cyclic heteroaryl, C.sub.2-10 alkenyl, C.sub.2-10 alkynyl, and C.sub.2-10 alkoxy. In the disclosure, the derivative is superior to the previous ?-elemene nitric oxide donor derivatives in terms of the design strategy, an alcohol amine structure that can enhance in vivo anti-tumor activity is introduced as a linker, which improves in vivo stability, and provides good therapeutic activity against malignant brain glioma against which effective therapeutic drugs is short in clinical practice.

##STR00001##

Claims

1. A 14-chloro-?-elemene nitric oxide donor derivative, and a pharmaceutically acceptable salt, a solvate, an enantiomer, or a diastereoisomer thereof, the 14-chloro-?-elemene nitric oxide donor derivative having a structural general formula as shown in formula (I): ##STR00035## wherein in formula (I), R.sup.1 represents a linear or cyclic alcohol amine structure containing nitrogen and oxygen atoms; and each of R.sup.2 and R.sup.3 is independently selected from the group consisting of C.sub.1-10 alkyl, C.sub.3-12 cycloalkyl, C.sub.6-12 aryl, 5- to 10-membered cyclic heteroaryl, C.sub.2-10 alkenyl, C.sub.2-10 alkynyl, and C.sub.2-10 alkoxy.

2. The 14-chloro-?-elemene nitric oxide donor derivative, and a pharmaceutically acceptable salt, a solvate, an enantiomer, or a diastereoisomer thereof of claim 1, wherein in formula (I): R.sup.1 represents a linear C.sub.2-5 alcohol amine structure containing nitrogen and oxygen atoms or a cyclic C.sub.5-6 alcohol amine structure containing nitrogen and oxygen atoms; and each of R.sup.2 and R.sup.3 is independently selected from the group consisting of C.sub.2-6 alkyl, C.sub.2-6 alkenyl, and C.sub.2-6 alkynyl.

3. The 14-chloro-?-elemene nitric oxide donor derivative, and a pharmaceutically acceptable salt, a solvate, an enantiomer, or a diastereoisomer thereof of claim 1, wherein in formula (I): R.sup.1 is any one selected from the group consisting of ##STR00036## and each of R.sup.2 and R.sup.3 is independently any one selected from the group consisting of CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2CH.sub.2CH.sub.2, CH?CH, CH.sub.2CH?CH, CH?CHCH.sub.2, CH.sub.2CH?CHCH.sub.2, CH.sub.2CH.sub.2CH?CH, CH?CHCH.sub.2CH.sub.2, CH.sub.2C?C, C?CCH.sub.2, CH.sub.2C?CCH.sub.2, CH.sub.2CH.sub.2C?CCH.sub.2, CH.sub.2CH.sub.2C?CCH.sub.2CH.sub.2, and CH.sub.2C?CCH.sub.2CH.sub.2.

4. The 14-chloro-?-elemene nitric oxide donor derivative, and a pharmaceutically acceptable salt, a solvate, an enantiomer, or a diastereoisomer thereof of claim 1, wherein the 14-chloro-?-elemene nitric oxide donor derivative is any one selected from the group consisting of compounds having a structure shown in formulas I-1 to I-6: ##STR00037## ##STR00038## and in the formulas I-1 to I-6, each of R.sup.2 and R.sup.3 is independently any one selected from the group consisting of C.sub.2-6 alkyl, C.sub.2-6 alkenyl, and C.sub.2-6 alkynyl.

5. The 14-chloro-?-elemene nitric oxide donor derivative, and a pharmaceutically acceptable salt, a solvate, an enantiomer, or a diastereoisomer thereof of claim 1, wherein the 14-chloro-?-elemene nitric oxide donor derivative is any one selected from the group consisting of compounds having a structure shown in formulas 1 to 24: ##STR00039## ##STR00040## ##STR00041## ##STR00042## ##STR00043## ##STR00044##

6. A method for treating a tumor, the method comprising administering the 14-chloro-?-elemene nitric oxide donor derivative, and a pharmaceutically acceptable salt, a solvate, an enantiomer, or a diastereoisomer thereof of claim 1 to a subject in need thereof.

7. The method of claim 6, wherein the tumor comprises lung cancer, colon cancer, or malignant brain glioma.

8. The 14-chloro-?-elemene nitric oxide donor derivative, and a pharmaceutically acceptable salt, a solvate, an enantiomer, or a diastereoisomer thereof of claim 2, wherein in formula (I): R.sup.1 is any one selected from the group consisting of ##STR00045## and each of R.sup.2 and R.sup.3 is independently any one selected from the group consisting of CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2CH.sub.2CH.sub.2, CH?CH, CH.sub.2CH?CH, CH?CHCH.sub.2, CH.sub.2CH?CHCH.sub.2, CH.sub.2CH.sub.2CH?CH, CH?CHCH.sub.2CH.sub.2, CH.sub.2C?C, C?CCH.sub.2, CH.sub.2C?CCH.sub.2, CH.sub.2CH.sub.2C?CCH.sub.2, CH.sub.2CH.sub.2C?CCH.sub.2CH.sub.2, and CH.sub.2C?CCH.sub.2CH.sub.2.

9. The method of claim 6, wherein in formula (I): R.sup.1 represents a linear C.sub.2-5 alcohol amine structure containing nitrogen and oxygen atoms or a cyclic C.sub.5-6 alcohol amine structure containing nitrogen and oxygen atoms; and each of R.sup.2 and R.sup.3 is independently selected from the group consisting of C.sub.2-6 alkyl, C.sub.2-6 alkenyl, and C.sub.2-6 alkynyl.

10. The method of claim 6, wherein in formula (I): R.sup.1 is any one selected from the group consisting of ##STR00046## and each of R.sup.2 and R.sup.3 is independently any one selected from the group consisting of CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2CH.sub.2, CH.sub.2CH.sub.2CH.sub.2CH.sub.2CH.sub.2, CH?CH, CH.sub.2CH?CH, CH?CHCH.sub.2, CH.sub.2CH?CHCH.sub.2, CH.sub.2CH.sub.2CH?CH, CH?CHCH.sub.2CH.sub.2, CH.sub.2C?C, C?CCH.sub.2, CH.sub.2C?CCH.sub.2, CH.sub.2CH.sub.2C?CCH.sub.2, CH.sub.2CH.sub.2C?CCH.sub.2CH.sub.2, and CH.sub.2C?CCH.sub.2CH.sub.2.

11. The method of claim 6, wherein the 14-chloro-?-elemene nitric oxide donor derivative is any one selected from the group consisting of compounds having a structure shown in formulas I-1 to I-6: ##STR00047## ##STR00048## and in the formulas I-1 to I-6, each of R.sup.2 and R.sup.3 is independently any one selected from the group consisting of C.sub.2-6 alkyl, C.sub.2-6 alkenyl, and C.sub.2-6 alkynyl.

12. The method of claim 6, wherein the 14-chloro-?-elemene nitric oxide donor derivative is any one selected from the group consisting of compounds having a structure shown in formulas 1 to 24: ##STR00049## ##STR00050## ##STR00051## ##STR00052## ##STR00053## ##STR00054##

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0030] FIG. 1 shows the advantages of the compound prepared in an embodiment of the present disclosure compared with that prepared in CN201710066664.1.

[0031] FIG. 2 shows the synthetic route of 13,14-dichloro-?-elemene.

[0032] FIG. 3 shows the synthetic routes of intermediates 5 to 10.

[0033] FIG. 4 shows the synthetic routes of intermediates 12, 13, 14a, 14b, 15a, and 15b.

[0034] FIG. 5 shows the synthetic routes of the ?-elemene nitric oxide donor derivative final products Ia-d, IIa-b, IIIa-d, IVa-d, Va-b, and VIa-b.

[0035] FIG. 6 shows the anti-tumor activities of ?-elemene and the product prepared in Example 4 against malignant brain glioma (n=5), in which (A) Body Weight, (B) Brain weight, (C) Bioluminescence signal intensity of brain tumor, and (D) Inhibitory rates of ?-elemene and the product prepared in Example 4.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0036] The present disclosure will be further described below with reference to the drawings and specific examples. It should be understood that these embodiments are intended to illustrate the present disclosure only but not limit the scope of the present disclosure. The operation methods without specific conditions in the following embodiments are generally in accordance with conventional conditions or the conditions recommended by the manufacturer.

(1) Preparation of Intermediate 4

[0037] As shown in the synthetic route in FIG. 2, NCS (1.34 g, 20 mmol), ytterbium trifluoromethanesulfonate (310 mg, 0.5 mmol), trimethylchlorosilane (54 mg, 0.5 mmol) were added in turn to a mixed solution of dichloromethane (8 mL) and tetrahydrofuran (2 mL) containing ?-elemene (1.02 g, 5 mmol) at 0? C., obtaining a reactant mixture. The reactant mixture reacted at 0? C. for 8 h. At the end of the reaction, the resulting reaction product mixture was distilled under reduced pressure to remove solvents, and then diluted by adding water (15 mL). The resulting mixture was subjected to extraction with ethyl acetate (4 mL) three times, and organic phases were combined. The organic phase after combining was washed with water (20 mL?2) and saturated saline (20 mL?2) in turn, and dried with anhydrous sodium sulfate, obtaining a dried organic phase. The dried organic phase was distilled under reduced pressure to remove solvent, obtaining a residue. The residue was purified by silica gel column chromatography (pure petroleum ether), obtaining a colorless liquid compound, i.e., intermediate 4 with a yield of 45%. The spectroscopy data is as follows.

.SUP.1.H NMR of Intermediate 4

[0038] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 5.85-5.72 (m, 1H), 5.28 (s, 1H), 5.18 (s, 1H), 5.04 (s, 1H), 4.98-4.89 (m, 3H), 4.15-4.05 (m, 3H), 3.97 (d, J=11.7 Hz, 1H), 2.35-2.21 (m, 2H), 1.77-1.42 (m, 6H), 0.99 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 149.6, 149.0, 147.5, 116.4, 113.5, 111.6, 51.1, 47.8, 47.5, 41.0, 39.8, 39.7, 33.8, 27.0, 15.8.

(2) Preparation of Intermediates 5 to 10

[0039] As shown in the synthetic route in FIG. 3, a corresponding alcohol amine reactant (1.3 mmol) and N,N-diisopropylethylamine (DIPEA, 172 mg, 1.3 mmol) were added in turn to N,N-dimethylformamide (DMF, 3 mL) containing the intermediate 4 (304 mg, 1.1 mmol) and they were stirred at 60? C. for 12. At the end of a reaction, the reaction product mixture was diluted with water (10 mL), and the resulting mixture was subjected to extraction with ethyl acetate (10 mL), obtaining an organic phase. The organic phase was combined, washed with water (10 mL) and saturated salt water (10 mL), and dried with anhydrous sodium sulfate, obtaining a residue. The residue was purified by silica gel column chromatography (dichloromethane:methanol of 150:1 v/v), obtaining a pale yellow liquid, i.e., intermediates 5 to 10. The spectroscopy data is as follows.

.SUP.1.H NMR of Intermediates 5 to 10

2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl))(methyl)amino)ethan-1-ol (5)

[0040] Light yellow liquid, yield 44%. .sup.1H NMR (400 MHz, CDCl.sub.3) ? 5.78 (dd, J=18.0, 10.3 Hz, 1H), 5.27 (s, 1H), 5.01-4.88 (m, 5H), 4.14-3.91 (m, 2H), 3.61 (t, J=5.4 Hz, 2H), 3.01 (s, 2H), 2.56-2.51 (m, 2H), 2.28 (dd, J=12.5, 3.4 Hz, 1H), 2.20 (s, 3H), 2.11 (t, J=11.8 Hz, 1H), 1.72-1.44 (m, 6H), 0.98 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 151.0, 149.3, 147.8, 116.2, 111.4, 111.3, 63.1, 59.0, 58.6, 51.0, 41.9, 41.7, 40.0, 39.9, 34.1, 27.3, 16.0. HRMS (ESI) calcd for C.sub.18H.sub.31ClNO 312.2089 [M+H].sup.+, found 312.2080.

2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)(isopropyl)amino)ethan-1-ol (6)

[0041] Light yellow liquid, yield 60%. .sup.1H NMR (400 MHz, CDCl.sub.3) ?5.77(dd, J=17.1, 11.2 Hz, 1H), 5.26 (s, 1H), 5.02-4.79 (m, 5H), 4.02 (dd, J=47.3, 11.7 Hz, 2H), 3.51 (s, 2H), 3.02 (d, J=18.7 Hz, 3H), 2.54 (s, 2H), 2.27 (dd, J=12.6, 3.1 Hz, 1H), 2.14 -2.02 (m, 1H), 1.71-1.41 (m, 6 H), 0.98 (d, J=7.2 Hz, 9H). .sup.13C NMR (100 MHz, CDCl.sub.3) ?149.2, 147.7, 116.3, 111.4, 58.5, 54.9, 51.2, 49.9, 49.1, 47.9, 41.7, 39.9, 40.0, 34.3, 27.3, 17.8, 17.7, 15.9. HRMS (ESI) calcd for C.sub.20H.sub.35ClNO 340.2402 [M+H].sup.+, found 340.2394.

(S)-1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)pyrrolidin-3-ol (7)

[0042] Light yellow liquid, yield 48%. .sup.1H NMR (400 MHz, CDCl.sub.3) ?5.77 (dd, J=17.1, 11.1 Hz, 1H), 5.26 (s, 1H), 5.05-4.80 (m, 5H), 4.30 (s, 1H), 4.09 (d, J=11.6 Hz, 1H), 3.96 (d, J=11.7 Hz, 1H), 3.05 (q, J=13.4 Hz, 2H), 2.85 (td, J=8.1, 7.2, 4.1 Hz, 1H), 2.66 (d, J=10.2 Hz, 1H), 2.55 (s, 1H), 2.43 (dd, J=10.2, 5.1 Hz, 1H), 2.31-2.07 (m, 4H), 1.69-1.41 (m, 6H), 0.96 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 151.4, 149.3, 147.7, 116.3, 111.3, 110.2, 71.5, 63.1, 60.9, 52.6, 51.1, 47.7, 42.0, 39.9, 39.8, 35.0, 34.0, 27.1, 15.8. HRMS (ESI) calcd for C.sub.19H.sub.31ClNO 324.2089 [M+H].sup.+, found 324.2083.

(1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-4-yl)methanol (8)

[0043] Light yellow liquid, yield 60%. .sup.1H NMR (400 MHz, CDCl.sub.3) ?5.79 (dd, J=17.9, 10.4 Hz, 1H), 5.26 (s, 1H), 4.98-4.86 (m, 5H), 4.09 (d, J=11.7 Hz, 1H), 3.97 (d, J =11.7 Hz, 1H), 3.50 (d, J=6.4 Hz, 2H), 2.90 (d, J=14.5 Hz, 4H), 2.32-2.23 (m, 1H), 2.13 (d, J=7.9 Hz, 1H), 1.88 (s, 2H), 1.75-1.57 (m, 7H), 1.53-1.43 (m, 4H), 0.98 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ?149.1, 147.6, 115.9, 110.9, 67.8, 63.5, 53.5, 53.3, 50.8, 47.6, 42.0, 39.7, 39.6, 38.5, 33.8, 28.6, 26.8, 15.6. HRMS (ESI) calcd for C.sub.21H.sub.35ClNO 352.2402 [M+H].sup.+, found 352.2384.

(1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-3-yl)methanol (9)

[0044] Light yellow liquid, yield 51%. .sup.1H NMR (400 MHz, CDCl.sub.3) ? 5.83-5.73 (m, 1H), 5.26 (s, 1H), 4.91 (dd, J=10.5, 6.4 Hz, 5H), 4.14-4.02 (m, 1H), 3.97 (d, J=11.7 Hz, 1H), 3.69-3.54 (m, 2H), 2.89 (d, J=3.9 Hz, 2H), 2.68 (d, J=9.7 Hz, 1H), 2.47 (s, 2H), 2.27 (dd, J=11.0, 5.0 Hz, 1H), 2.09 (ddt, J=12.2, 8.2, 4.3 Hz, 2H), 1.82-1.74 (m, 2H), 1.70-1.41 (m, 9H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ?150.7, 149.4, 147.8, 147.8, 116.2, 111.3, 111.0, 67.5, 64.1, 57.8, 54.4, 51.0, 47.8, 42.1, 39.9, 39.9, 34.0, 27.8, 27.0, 24.6, 15.8. HRMS (ESI) calcd for C.sub.21H.sub.35ClNO 352.2402 [M+H].sup.+, found 352.2412.

2-((4-(2-(4-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperazin-1-yl)ethan-1-ol (10)

[0045] Light yellow liquid, yield 44%. .sup.1H NMR (400 MHz, CDCl.sub.3) ?5.78 (dd, J=18.0, 10.2 Hz, 1H), 5.26 (s, 1H), 4.97-4.85 (m, 5H), 4.09 (d, J=11.5 Hz, 1H), 3.96 (d, J=11.6 Hz, 1H), 3.61 (t, J=5.4 Hz, 2H), 2.91 (d, J=4.2 Hz, 2H), 2.62-2.38 (m, 10H), 2.30-2.25 (m, 1H), 2.15-2.08 (m, 1H), 1.64-1.43 (m, 6H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ?150.7, 149.4, 147.8, 116.3, 111.3, 111.0, 63.5, 59.4, 57.8, 53.3, 53.1, 51.2, 47.7, 42.2, 39.9, 39.9, 34.0, 27.1, 15.8. HRMS (ESI) calcd for C.sub.21H.sub.36ClN.sub.2O 367.2511 [M+H].sup.+, found 367.2503.

(3) Preparation of Intermediates 12 and 13

[0046] At 0? C., 25% NaOH aqueous solution (0.2 mL) was added slowly dropwise to a tetrahydrofuran solution containing 3,4-bis(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide (183 mg, 0.5 mmol). After the dropwise addition, the resulting mixture was stirred for further 10 min, and a reactant ethylene glycol (124 mg, 2 mmol) was added thereto. The resulting reactant mixture reacted for 6 h at room temperature. The reaction product mixture was distilled under reduced pressure to remove solvent, and then diluted by adding water (15 mL). The resulting mixture was subjected to extraction with dichloromethane (20 mL) for three times, and organic phases were combined. The organic phase after combining was washed with water (20 mL?2) and saturated saline (20 mL?2) in turn, and dried with anhydrous sodium sulfate, obtaining a dried organic phase. The dried organic phase was distillated under reduced pressure to remove solvent, obtaining a residue. The residue was purified by silica gel column chromatography (dichloromethane:methanol of 400:1, v/v), obtaining an intermediate 12 (72 mg, 48%).

[0047] m.p. 128-130? C. .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=8.2 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 4.59-4.49 (m, 2H), 4.08-4.02 (m, 2H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 159.1, 137.8, 135.9, 129.9, 128.7, 110.7, 73.0, 60.5. HRMS (ESI) calcd for C.sub.10H.sub.10N.sub.2NaO.sub.6S 309.0152 [M+Na].sup.+, found 309.0142.

[0048] As shown in FIG. 4, intermediate 13 was prepared according to the methods as described above, except that the reactant ethylene glycol was replaced with 1,4-butynediol.

.SUP.1.H NMR Data of Intermediate 13

4-((4-hydroxybut-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide (13)

[0049] White solid, yield 53%, m.p. 116-118? C. .sup.1H NMR (400 MHz, CDCl.sub.3) ?8.07 (d, J=7.7 Hz, 2H), 7.76 (t, J=7.4 Hz, 1H), 7.63 (t, J=7.7 Hz, 2H), 5.10 (s, 2H), 4.34 (s, 2H). .sup.13C NMR (100 MHz, CDCl3) ?158.2, 138.0, 135.9, 129.8, 128.8, 110.8, 88.3, 77.7, 59.0, 51.1. HRMS (ESI) calcd for C.sub.12H.sub.10N.sub.2NaO.sub.6S 333.0512 [M+Na].sup.+, found 333.0512.

(4) Preparation of Intermediates 14a, 14b, 15a, and 15b

[0050] As shown in FIG. 4, reactants succinic anhydride (76 mg, 0.76 mmol) and 4-dimethylaminopyridine DMAP (38 mg, 0.32 mmol) were added in turn to a solution of dichloromethane (5 mL) containing the intermediate 12 (182 mg, 0.63 mmol). The resulting mixture was stirred and reacted at room temperature for 6 h, obtaining a mixed solution. At room temperature, the reaction was quenched by adding water (5 mL). The stirring was continued for 10 min. The resulting mixture was subjected to extraction with dichloromethane (5 mL) for three times, and organic phases were combined. The organic phase after combining was washed with water (20 mL?2) and saturated saline (20 mL?2) in turn, and dried with anhydrous sodium sulfate, obtaining a dried organic phase. The dried organic phase was distilled under reduced pressure, obtaining a residue. The residue was purified by silica gel column chromatography (dichloromethane:methanol of 150:1, v/v), obtaining a white solid, i.e., intermediate 14a (167 mg, 67%).

[0051] m.p. 118-120? C. .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.3 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.9 Hz, 2H), 4.72-4.59 (m, 2H), 4.57-4.44 (m, 2H), 2.92-2.51 (m, 4H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 176.9, 171.9, 158.8, 138.2, 135.8, 129.8, 128.8, 110.6, 69.0, 61.6, 29.0, 28.9. HRMS (ESI) calcd for C.sub.14H.sub.14N.sub.2NaO.sub.9S 409.0312 [M+Na].sup.+, found 409.0295.

[0052] As shown in in FIG. 4, intermediates 14b, 15a, and 15b were prepared according to methods described above, except that the reactants were replaced accordingly.

.SUP.1.H NMR Data of Intermediates 14b, 15a, and 15b

4-(2((4-carboxybutyryl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide (14b)

[0053] White solid, yield 53%, m.p. 91-93? C. .sup.1H NMR (400 MHz, CDCl.sub.3) ?8.06 (d, J=8.1 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.62 (t, J=7.8 Hz, 2H), 4.66-4.60 (m, 2H), 4.55-4.48 (m, 2H), 2.47 (q, J=7.0 Hz, 4H), 1.98 (p, J=7.2 Hz, 2H). .sup.13C NMR (100 MHz, CDCl.sub.3) ?178.6, 172.7, 158.8, 138.0, 135.8, 129.8, 128.8, 110.6, 69.0, 61.3, 33.0, 32.9, 19.7. HRMS (ESI) calcd for C.sub.15H.sub.16N.sub.2NaO.sub.9S 423.0469 [M+Na].sup.+, found 423.0465.

4-((4-((3-carboxylpropionyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide (15a)

[0054] White solid, yield 54%, m.p. 106-108? C. .sup.1H NMR (400 MHz, CDCl.sub.3) ?8.07 (d, J=7.4 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.9 Hz, 2H), 5.10 (s, 2H), 4.77 (s, 2H), 2.70 (dt, J=8.1, 4.7 Hz, 4H). .sup.13C NMR (100 MHz, CDCl.sub.3) ?177.2, 171.4, 158.1, 138.1, 135.9, 129.9, 128.8, 110.8, 83.9, 78.8, 58.8, 52.3, 28.8, 28.8. HRMS (ESI) calcd for C.sub.16H.sub.14N.sub.2NaO.sub.9S 433.0312 [M+Na].sup.+, found 433.0317.

4-(4((4-carboxybutyryl)oxy)but-2-yn-1-yloxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide (15b)

[0055] White solid, yield 54%, m.p. 94-96? C. .sup.1H NMR (400 MHz, CDCl.sub.3) ?8.07 (d, J=7.6 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.10 (s, 2H), 4.74 (s, 2H), 2.46 (td, J=7.3, 4.9 Hz, 4H), 1.97 (p, J=7.3 Hz, 2H). .sup.13C NMR (100 MHz, CDCl.sub.3) ?179.0, 172.1, 158.0, 137.7, 135.8, 129.8, 128.7, 110.6, 83.9, 78.6, 58.6, 52.0, 32.8, 19.6. HRMS (ESI) calcd for C.sub.16H.sub.14N.sub.2NaO.sub.9S 447.0469 [M+Na].sup.+, found 447.0486.

(5) Preparation of the ?-Elemene Nitric Oxide Donor Derivative Final Products Ia-Id, IIa-IIb, IIIa-IIIb, IVa-IVd, Va-Vb, and VIa-VIb

[0056] As shown in FIG. 5, the intermediates 5 to 10 (0.08 mmol), NO donor intermediates 14a, 14b, 15a, and 15b (0.10 mmol), 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDCI, 0.12 mmol) and DMAP (0.01 mmol) were stirred and reacted in anhydrous dichloromethane (2 mL) at room temperature for 8 h. The reaction was monitored by thin-layer chromatography. The resulting reaction mixture was diluted with dichloromethane (5 mL), obtaining an organic layer. The organic layer was washed with water and brine in turn, dried with anhydrous sodium sulfate, obtaining a dried organic layer. The dried organic layer was distilled under reduced pressure to remove solvent, obtaining a residue. The residue was purified by silica gel column chromatography (dichloromethane/methanol), obtaining a light yellow liquid.

[0057] Each intermediate used can be prepared according to (1)-(4) above and will not be repeated here.

[0058] The technical solutions provided by the present disclosure will be described in detail below with reference to the examples, but they are not to be construed as limiting the scope of protection of the present disclosure.

Example 1

[0059] ##STR00011##

[0060] 4-(2-((4-(2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl))(methyl)amino)ethyoxyl)-4-oxobutanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared according to the following procedures.

[0061] Intermediate 14a (39 mg, 0.101 mmol), DMAP (1 mg, 0.008 mmol), and EDCI (24 mg, 0.126 mmol) were added in turn to a dichloromethane (1.5 mL) solution containing intermediate 5 (26 mg, 0.084 mmol), obtaining a reactant mixture. The reactant mixture was stirred at room temperature for 8 h. The reaction product mixture was diluted with dichloromethane (5 mL), washed with water (10 mL?2) and saturated salt solution (10 mL?2) in turn, and dried with anhydrous sodium sulfate, obtaining a dried reaction product mixture. The dried reaction product mixture was distilled under reduced pressure to remove solvent, obtaining a residue. The residue was purified by silica gel column chromatography (a volume ratio of dichloromethane:methanol being 400:1), obtaining a light yellow liquid with a yield of 69%.

[0062] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.8 Hz, 2H), 7.75 (d, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.8, 10.4 Hz, 1H), 5.26 (s, 1H), 5.02-4.87 (m, 5H), 4.65-4.59 (m, 2H), 4.57-4.48 (m, 2H), 4.21 (t, J=5.8 Hz, 2H), 4.09 (d, J=11.7 Hz, 1H), 3.96 (d, J=11.8 Hz, 1H), 3.01 (s, 2H), 2.63 (d, J=5.5 Hz, 6H), 2.32-2.22 (m, 4H), 1.66-1.42 (m, 6H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.0, 172.0, 158.7, 149.2, 147.7, 138.1, 135.6, 129.7, 128.6, 116.1, 111.2, 110.4, 77.0, 68.9, 62.5, 61.4, 55.3, 50.8, 47.9, 42.6, 41.5, 39.8, 33.8, 29.0, 28.9, 27.1, 15.8. HRMS (ESI) calcd for C.sub.32H.sub.43ClN.sub.3O.sub.9S 680.2403 [M+H].sup.+, found 680.2441.

Example 2

[0063] ##STR00012##

[0064] 4-(2-((5-(2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)(methyl)amino)ethyoxyl)-5-oxopentanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 1, except that: succinic anhydride used in (3) was replaced with glutaric anhydride.

[0065] In this example, a yellow waxy liquid was obtained, with a yield of 71%.

[0066] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=8.5 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.9 Hz, 2H), 5.87-5.71 (m, 1H), 5.26 (s, 1H), 5.01-4.86 (m, 5H), 4.67-4.60 (m, 2H), 4.53-4.46 (m, 2H), 4.17 (t, J=5.7 Hz, 2H), 4.09 (d, J=11.6 Hz, 1H), 3.96 (d, J=11.7 Hz, 1H), 2.95 (s, 2H), 2.57 (t, J=5.1 Hz, 2H), 2.43 (dt, J=17.0, 7.3 Hz, 4H), 2.28 (dd, J=11.9, 4.0 Hz, 1H), 2.16-2.07 (m, 1H), 2.02-1.93 (m, 2H), 1.65-1.35 (m, 6H), 0.96 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 173.0, 172.8, 158.8, 151.4, 149.4, 147.8, 138.1, 135.8, 129.8, 128.7, 116.3, 110.9, 110.5, 69.0, 63.3, 62.5, 61.2, 55.4, 51.1, 47.7, 42.9, 41.5, 39.9, 39.9, 33.9, 33.2, 33.1, 27.1, 20.0, 15.8. HRMS (ESI) calcd for C.sub.33H.sub.45ClN.sub.3O.sub.9S 694.2560 [M+H].sup.+, found 694.2562.

Example 3

[0067] ##STR00013##

[0068] 4-((4-((4-(2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)(methyl)amino)ethyoxyl)-4-oxobutanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 1, except that: ethylene glycol used in (3) was replaced with 2-butyne-1,4-diol.

[0069] In this example, a yellow waxy liquid was obtained, with a yield of 74%.

[0070] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.4 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.64 (t, J=7.9 Hz, 2H), 5.78 (dd, J=16.9, 11.3 Hz, 1H), 5.26 (s, 1H), 5.10 (s, 2H), 5.05-4.85 (m, 5H), 4.76 (s, 2H), 4.20 (t, J=5.6 Hz, 2H), 4.12-4.06 (m, 1H), 3.96 (d, J=11.7 Hz, 1H), 2.97 (s, 2H), 2.70-2.56 (m, 6H), 2.31-2.09 (m, 5H), 1.70-1.41 (m, 6H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.2, 171.6, 158.0, 151.3, 149.4, 149.4, 147.7, 137.9, 135.9, 129.9, 128.8, 116.3, 111.3, 110.7, 83.9, 78.7, 63.2, 62.8, 58.7, 55.3, 52.3, 51.1, 47.7, 42.8, 41.4, 39.9, 39.9, 33.9, 29.0, 28.9, 27.1, 15.8. HRMS (ESI) calcd for C.sub.34H.sub.43ClN.sub.3O.sub.9S 704.2403 [M+H].sup.+, found 704.2408.

Example 4

[0071] ##STR00014##

[0072] 4-((4-((5-(2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)(methyl)amino)ethyoxyl)-5-oxopentyloxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 3, except that: succinic anhydride used in (3) was replaced with glutaric anhydride.

[0073] In this example, a yellow waxy liquid was obtained, with a yield of 73%.

[0074] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.4 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.77 (dd, J=17.1, 11.2 Hz, 1H), 5.25 (s, 1H), 5.09 (s, 2H), 4.98-4.83 (m, 5H), 4.73 (s, 2H), 4.17 (t, J=5.8 Hz, 2H), 4.09 (d, J=11.7 Hz, 1H), 3.96 (d, J=11.7 Hz, 1H), 2.96 (s, 2H), 2.57 (t, J=5.5 Hz, 2H), 2.41 (dt, J=17.1, 7.3 Hz, 4H), 2.31-2.18 (m, 4H), 2.12 (s, 1H), 1.96 (p, J=7.1 Hz, 2H), 1.68-1.41 (m, 6H), 0.96 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.9, 172.2, 158.0, 151.3, 149.3, 147.7, 137.8, 135.9, 129.8, 128.8, 116.3, 111.3, 110.9, 110.7, 84.0, 78.6, 63.2, 62.4, 58.7, 55.4, 52.0, 51.1, 47.7, 39.9, 33.8, 33.2, 33.0, 27.1, 20.0, 15.8. HRMS (ESI) calcd for C.sub.35H.sub.45ClN.sub.3O.sub.9S 718.2560 [M+H].sup.+, found 718.2553.

Example 5

[0075] ##STR00015##

[0076] 4-(2-((4-(2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)(isopropyl)amino)ethyoxyl)-4-oxobutanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 1, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 2-(isopropylamino)ethanol.

[0077] In this example, a yellow waxy liquid was obtained, with a yield of 58%.

[0078] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.5 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.77 (dd, J=17.6, 10.6 Hz, 1H), 5.25 (s, 1H), 5.03-4.84 (m, 5H), 4.66-4.58 (m, 2H), 4.56-4.47 (m, 2H), 4.11-3.93 (m, 4H), 3.06-2.82 (m, 3H), 2.72-2.56 (m, 6H), 2.30-2.09 (m, 2H), 1.65-1.40 (m, 6H), 1.02-0.90 (m, 9H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.2, 172.1, 158.7, 149.8, 149.3, 147.7, 138.0, 135.7, 129.7, 128.7, 116.2, 111.2, 110.4, 110.0, 68.9, 64.0, 61.4, 55.6, 51.2, 50.2, 47.7, 41.3, 39.9, 39.9, 34.0, 28.9, 28.9, 27.1, 18.0, 17.7, 15.7. HRMS (ESI) calcd for C.sub.34H.sub.47ClN.sub.3O.sub.9S 708.2716 [M+H].sup.+, found 708.2703.

Example 6

[0079] ##STR00016##

[0080] 4-(2-((5-(2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)(isopropyl)amino)ethyoxyl)-5-oxopentyloxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 2, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 2-(isopropylamino)ethanol.

[0081] In this example, a yellow waxy liquid was obtained, with a yield of 68%.

[0082] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=7.4 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.62 (t, J=7.8 Hz, 2H), 5.77 (dd, J=16.9, 11.3 Hz, 1H), 5.25 (s, 1H), 5.03-4.82 (m, 5H), 4.65-4.56 (m, 2H), 4.54-4.43 (m, 2H), 4.12-3.91 (m, 4H), 3.08-2.79 (m, 3H), 2.58 (t, J=6.6 Hz, 2H), 2.42 (dt, J=26.0, 7.3 Hz, 4H), 2.26 (dd, J=12.7, 3.4 Hz, 1H), 2.13 (t, J=11.3 Hz, 1H), 1.97 (p, J=7.3 Hz, 2H), 1.66-1.41 (m, 6H), 1.01-0.87 (m, 9H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.9, 172.8, 158.8, 149.8, 149.3, 147.8, 138.0, 135.8, 129.8, 128.7, 116.3, 111.3, 110.5, 110.1, 69.0, 63.8, 61.2, 55.6, 51.2, 50.2, 47.8, 41.4, 40.0, 39.9, 34.1, 33.2, 33.1, 27.1, 20.0, 18.1, 17.8, 15.8. HRMS (ESI) calcd for C.sub.35H.sub.48ClN.sub.3O.sub.9S 722.2873 [M+H].sup.+, found 722.2873.

Example 7

[0083] ##STR00017##

[0084] 4-((4-((4-(2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)(isopropyl)amino)ethyoxyl)-4-oxobutanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 3, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 2-(isopropylamino)ethanol.

[0085] In this example, a yellow waxy liquid was obtained, with a yield of 47%.

[0086] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.08 (d, J=7.7 Hz, 2H), 7.77 (t, J=7.4 Hz, 1H), 7.64 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.1, 11.1 Hz, 1H), 5.25 (s, 1H), 5.10 (s, 2H), 4.76 (s, 2H), 4.18-3.91 (m, 4H), 2.98 (d, J=45.6 Hz, 3H), 2.65 (dt, J=10.6, 5.1 Hz, 6H), 2.31-2.11 (m, 2H), 1.64-1.41 (m, 6H), 0.97 (s, 9H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.1, 171.6, 158.1, 149.4, 147.9, 138.1, 135.8, 129.9, 128.8, 116.2, 111.3, 110.7, 84.0, 78.8, 58.7, 55.7, 52.2, 51.1, 48.0, 47.9, 41.5, 40.0, 34.2, 29.1, 29.0, 27.3, 22.8, 18.1, 17.8, 15.9. HRMS (ESI) calcd for C.sub.36H.sub.47ClN.sub.3O.sub.9S 732.2716 [M+H].sup.+, found 732.2711.

Example 8

[0087] ##STR00018##

[0088] 4-((4-((5-(2-((2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)(isopropylamino)ethyoxyl)-5-oxopentanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 4, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 2-(isopropylamino)ethanol.

[0089] In this example, a yellow waxy liquid was obtained, with a yield of 53%.

[0090] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.4 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.0, 11.2 Hz, 1H), 5.25 (s, 1H), 5.09 (s, 2H), 5.04-4.81 (m, 5H), 4.74 (s, 2H), 4.13-3.91 (m, 4H), 3.09-2.79 (m, 3H), 2.59 (t, J=6.2 Hz, 2H), 2.40 (dt, J=26.3, 7.4 Hz, 5H), 2.26 (dd, J=12.2, 3.5 Hz, 1H), 2.14 (s, 1H), 1.96 (p, J=7.3 Hz, 2H), 1.66-1.41 (m, 6H), 1.06-0.89 (m, 9H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.7, 172.0, 157.9, 149.3, 147.7, 137.9, 135.7, 129.7, 128.7, 116.1, 111.1, 110.6, 110.0, 84.0, 78.5, 63.7, 58.6, 55.6, 51.8, 51.0, 50.3, 47.9, 47.9, 41.4, 39.9, 39.9, 34.1, 33.2, 33.0, 27.1, 20.0, 18.0, 17.7, 15.8. HRMS (ESI) calcd for C.sub.37H.sub.49ClN.sub.3O.sub.9S 746.2873 [M+H].sup.+, found 746.2871.

Example 9

[0091] ##STR00019##

[0092] 4-(2-((4-(((S)-1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)pyrrolidine-3-ly)oxy)-4-oxobutanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 1, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with (R)-3-hydroxypyrrolidine.

[0093] In this example, a yellow waxy liquid was obtained, with a yield of 68%.

[0094] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=7.5 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.77 (dd, J=17.1, 11.2 Hz, 1H), 5.26 (s, 1H), 5.17 (s, 1H), 5.00-4.83 (m, 5H), 4.66-4.57 (m, 2H), 4.56-4.48 (m, 2H), 4.09 (d, J=11.6 Hz, 1H), 3.96 (d, J=11.7 Hz, 1H), 3.12 (d, J=13.4 Hz, 1H), 2.99 (d, J=13.4 Hz, 1H), 2.76-2.60 (m, 7H), 2.40 (s, 1H), 2.31-2.19 (m, 2H), 1.87-1.79 (m, 1H), 1.67-1.40 (m, 6H), 0.96 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.2, 172.2, 158.8, 149.3, 147.7, 138.0, 135.8, 129.8, 128.7, 116.2, 111.3, 110.5, 74.7, 68.9, 61.5, 60.8, 60.0, 53.0, 51.1, 47.6, 42.0, 39.9, 39.8, 34.0, 31.9, 29.2, 29.0, 27.1, 15.8. HRMS (ESI) calcd for C.sub.33H.sub.43ClN.sub.3O.sub.9S 692.2403 [M+H].sup.+, found 692.2394.

Example 10

[0095] ##STR00020##

[0096] 4-(2-((5-(((S)-1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)pyrrolidine-3-ly)oxy)-5-oxopentanoyl)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 2, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with (R)-3-hydroxypyrrolidine.

[0097] In this example, a yellow waxy liquid was obtained, with a yield of 62%.

[0098] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.05 (d, J=7.5 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.77 (dd, J=17.0, 11.2 Hz, 1H), 5.25 (s, 1H), 5.16 (s, 1H), 5.03-4.85 (m, 5H), 4.66-4.59 (m, 2H), 4.53-4.46 (m, 2H), 4.09 (d, J=11.6 Hz, 1H), 3.96 (d, J=11.7 Hz, 1H), 3.17-2.91 (m, 2H), 2.71 (d, J=6.6 Hz, 2H), 2.59 (d, J=9.8 Hz, 1H), 2.42 (dt, J=23.1, 7.3 Hz, 5H), 2.31-2.19 (m, 2H), 2.11 (s, 1H), 2.00-1.92 (m, 2H), 1.85-1.76 (m, 1H), 1.67-1.40 (m, 6H), 0.96 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.9, 172.8, 158.8, 149.3, 147.7, 138.0, 135.8, 129.8, 128.7, 116.2, 111.3, 110.5, 74.4, 69.0, 61.2, 60.9, 60.0, 53.0, 51.1, 47.6, 42.0, 39.9, 39.8, 34.0, 33.4, 33.1, 32.0, 27.1, 20.0, 15.8. HRMS (ESI) calcd for C.sub.34H.sub.45ClN.sub.3O.sub.9S 706.2560 [M+H].sup.+, found 706.2555.

Example 11

[0099] ##STR00021##

[0100] 4-((4-((4-(((S)-1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)pyrrolidine-3-ly)oxy)-4-oxobutanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 3, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with (R)-3-hydroxypyrrolidine.

[0101] In this example, a yellow waxy liquid was obtained, with a yield of 53%.

[0102] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.4 Hz, 2H), 7.77 (t, J=7.5 8Hz, 1H), 7.63 (t, J=7.9 Hz, 2H), 5.78 (dd, J=17.9, 10.3 Hz, 1H), 5.26 (s, 1H), 5.19 (s, 1H), 5.09 (s, 2H), 5.02-4.84 (m, 5H), 4.76 (s, 2H), 4.09 (d, J=11.4 Hz, 1H), 3.97 (d, J=11.8 Hz, 1H), 3.16-2.95 (m, 2H), 2.76-2.60 (m, 7H), 2.41 (s, 1H), 2.31-2.08 (m, 3H), 1.88-1.79 (m, 1H), 1.68-1.43 (m, 6H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.0, 171.6, 158.1, 149.4, 147.8, 147.8, 138.0, 135.8, 129.8, 128.8, 116.2, 111.3, 110.7, 84.0, 78.8, 74.8, 60.9, 60.0, 58.7, 53.0, 52.2, 51.0, 47.9, 42.2, 39.9, 39.9, 34.1, 32.0, 29.2, 29.0, 27.2, 15.9. HRMS (ESI) calcd for C.sub.35H.sub.43ClN.sub.3O.sub.9S 716.2403 [M+H].sup.+, found 716.2400.

Example 12

[0103] ##STR00022##

[0104] 4-((4-((5-(((S)-1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)pyrrolidine-3-ly)oxy)-5-oxopentanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 4, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with (R)-3-hydroxypyrrolidine.

[0105] In this example, a yellow waxy liquid was obtained, with a yield of 63%.

[0106] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.4 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.9, 10.3 Hz, 1H), 5.26 (s, 1H), 5.17 (td, J=6.2, 3.2 Hz, 1H), 5.09 (s, 2H), 5.01-4.82 (m, 5H), 4.74 (s, 2H), 4.09 (d, J=11.5 Hz, 1H), 3.96 (d, J=11.8 Hz, 1H), 3.11 (d, J=13.4 Hz, 1H), 2.99 (d, J=13.4 Hz, 1H), 2.78-2.66 (m, 2H), 2.60 (d, J=10.9 Hz, 1H), 2.40 (dt, J=23.9, 7.3 Hz, 5H), 2.31-2.17 (m, 2H), 2.10 (dq, J=7.6, 5.3, 3.5 Hz, 1H), 1.95 (p, J=7.3 Hz, 2H), 1.81 (dq, J=13.7, 8.7, 6.9 Hz, 1H), 1.70-1.39 (m, 6H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.8, 172.2, 158.0, 151.1, 149.3, 147.7, 137.8, 135.9, 129.8, 128.7, 116.2, 111.3, 110.7, 110.4, 84.0, 78.6, 74.4, 60.8, 60.0, 58.7, 53.0, 52.0, 51.0, 47.6, 42.0, 39.9, 39.8, 34.0, 33.3, 33.0, 31.9, 27.1, 20.0, 15.8. HRMS (ESI) calcd for C.sub.36H.sub.45ClN.sub.3O.sub.9S 730.2560 [M+H].sup.+, found 730.255.

Example 13

[0107] ##STR00023##

[0108] 4-(2-((4-((1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-4-yl)methoxyl)-4-oxobutanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 1, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 4-(hydroxymethyl)piperidine.

[0109] In this example, a yellow waxy liquid was obtained, with a yield of 67%.

[0110] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=7.7 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.1, 11.1 Hz, 1H), 5.25 (s, 1H), 4.97-4.84 (m, 5H), 4.66-4.56 (m, 2H), 4.57-4.47 (m, 2H), 4.13-3.88 (m, 3H), 2.88 (d, J=17.1 Hz, 4H), 2.68 (s, 4H), 2.34-2.20 (m, 1H), 2.12 (s, 1H), 1.85 (s, 2H), 1.70-1.38 (m, 10H), 0.96 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.25, 172.15, 158.69, 149.29, 147.72, 137.94, 135.74, 129.73, 128.66, 116.14, 111.18, 110.43, 69.29, 68.86, 63.64, 61.42, 53.42, 53.28, 51.08, 47.62, 42.10, 39.85, 39.74, 35.34, 33.91, 28.94, 28.92, 26.95, 15.69. HRMS (ESI) calcd for C.sub.35H.sub.47ClN.sub.3O.sub.9S 720.2716 [M+H].sup.+, found 720.2709.

Example 14

[0111] ##STR00024##

[0112] 4-(2-((5-((1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-4-yl)methoxyl)-5-oxopentanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 2, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 4-(hydroxymethyl)piperidine.

[0113] In this example, a yellow waxy liquid was obtained, with a yield of 70%.

[0114] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=7.4 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.62 (t, J=7.9 Hz, 2H), 5.78 (dd, J=18.0, 10.3 Hz, 1H), 5.26 (s, 1H), 5.01-4.85 (m, 5H), 4.72-4.59 (m, 2H), 4.54-4.46 (m, 2H), 4.08 (d, J=11.7 Hz, 1H), 3.98-3.90 (m, 3H), 3.03-2.80 (m, 4H), 2.42 (dt, J=18.4, 7.3 Hz, 4H), 2.31-2.23 (m, 1H), 2.15-2.07 (m, 1H), 2.03-1.86 (m, 4H), 1.68-1.42 (m, 10H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 173.0, 172.8, 158.8, 149.4, 147.8, 138.1, 135.8, 129.8, 128.7, 116.2, 111.3, 110.5, 69.0, 69.0, 63.6, 61.2, 53.4, 53.3, 51.1, 47.8, 42.2, 39.9, 39.8, 35.4, 34.0, 33.3, 33.2, 29.8, 28.8, 27.1, 20.1, 15.8. HRMS (ESI) calcd for C.sub.36H.sub.49ClN.sub.3O.sub.9S 734.2873 [M+H].sup.+, found 734.889.

Example 15

[0115] ##STR00025##

[0116] 4-((4-((4-((1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-4-yl)methoxyl)-4-oxobutanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 3, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 4-(hydroxymethyl)piperidine.

[0117] In this example, a yellow waxy liquid was obtained, with a yield of 65%.

[0118] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.4 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.9, 10.4 Hz, 1H), 5.26 (s, 1H), 5.09 (s, 2H), 5.02-4.85 (m, 5H), 4.76 (s, 2H), 4.08 (d, J=11.4 Hz, 1H), 3.99-3.94 (m, 3H), 2.98 (s, 4H), 2.66 (q, J=3.7 Hz, 4H), 2.30-2.25 (m, 1H), 2.17-2.11 (m, 1H), 1.97 (d, J=17.5 Hz, 2H), 1.71-1.43 (m, 10H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.1, 171.6, 158.1, 149.3, 147.9, 138.1, 135.8, 129.8, 128.8, 116.1, 111.3, 110.7, 84.0, 78.8, 69.1, 65.7, 63.5, 58.7, 53.4, 53.3, 52.2, 50.9, 48.0, 42.3, 39.9, 39.9, 35.3, 34.1, 29.8, 29.1, 29.0, 27.2, 15.9. HRMS (ESI) calcd for C.sub.37H.sub.47ClN.sub.3O.sub.9S 744.2716 [M+H].sup.+, found 744.2700.

Example 16

[0119] ##STR00026##

[0120] 4-((4-((5-((1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-4-yl)methoxyl)-5-oxopentanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 4, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 4-(hydroxymethyl)piperidine.

[0121] In this example, a yellow waxy liquid was obtained, with a yield of 60%.

[0122] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.5 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.5, 10.8 Hz, 1H), 5.25 (s, 1H), 5.09 (s, 2H), 5.00-4.85 (m, 5H), 4.74 (s, 2H), 4.09 (d, J=11.6 Hz, 1H), 4.01-3.87 (m, 3H), 2.89 (d, J=17.0 Hz, 4H), 2.41 (dt, J=18.4, 7.3 Hz, 5H), 2.30-2.23 (m, 1H), 2.16-2.06 (m, 1H), 2.04-1.77 (m, 5H), 1.73-1.39 (m, 10H), 0.96 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 173.0, 172.2, 158.0, 149.4, 147.8, 137.8, 135.9, 129.8, 128.8, 116.2, 111.3, 110.7, 84.0, 78.6, 76.8, 69.1, 63.7, 58.7, 53.5, 53.4, 52.0, 51.1, 47.7, 42.2, 39.9, 39.8, 35.4, 34.0, 33.2, 33.0, 29.0, 27.0, 20.1, 15.8. HRMS (ESI) calcd for C.sub.38H.sub.49ClN.sub.3O.sub.9S 758.2873 [M+H].sup.+, found 758.2866.

Example 17

[0123] ##STR00027##

[0124] 4-(2-((4-((1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-3-yl)methoxyl)-4-oxobutanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 1, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 3-(hydroxymethyl)piperidine.

[0125] In this example, a yellow waxy liquid was obtained, with a yield of 70%.

[0126] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.4 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.9 Hz, 2H), 5.78 (dd, J=17.9, 10.3 Hz, 1H), 5.26 (s, 1H), 4.98-4.82 (m, 5H), 4.65-4.60 (m, 2H), 4.54-4.50 (m, 2H), 4.14-3.87 (m, 4H), 2.88 (s, 2H), 2.67 (t, J=3.8 Hz, 6H), 2.32-2.23 (m, 1H), 2.11 (s, 1H), 1.91 (s, 2H), 1.78-1.41 (m, 12H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.3, 172.2, 158.8, 151.1, 149.4, 147.8, 138.1, 135.8, 129.8, 128.8, 116.2, 111.3, 111.3, 110.5, 69.0, 67.7, 64.0, 61.5, 57.0, 54.2, 51.1, 47.8, 42.2, 39.9, 39.9, 35.7, 34.1, 34.0, 29.0, 27.3, 27.1, 24.6, 15.8. HRMS (ESI) calcd for C.sub.35H.sub.47ClN.sub.3O.sub.9S 720.2716 [M+H].sup.+, found 720.2721.

Example 18

[0127] ##STR00028##

[0128] 4-(2-((5-((1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-3-yl)methoxyl)-5-oxopentanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 2, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 3-(hydroxymethyl)piperidine.

[0129] In this example, a yellow waxy liquid was obtained, with a yield of 74%.

[0130] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=7.5 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.77 (dd, J=17.3, 10.9 Hz, 1H), 5.25 (s, 1H), 4.98-4.80 (m, 5H), 4.68-4.57 (m, 2H), 4.55-4.45 (m, 2H), 4.09 (d, J=11.6 Hz, 1H), 3.94 (dd, J=21.4, 11.3 Hz, 3H), 2.99-2.54 (m, 4H), 2.42 (dt, J=20.6, 7.3 Hz, 4H), 2.30-2.23 (m, 1H), 2.11 (s, 1H), 1.97 (p, J=7.3 Hz, 4H), 1.76-1.36 (m, 10H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 173.0, 172.8, 158.8, 149.4, 147.8, 138.0, 135.8, 129.8, 128.7, 116.2, 111.3, 110.5, 69.0, 67.3, 63.9, 61.2, 57.0, 54.2, 51.1, 47.8, 42.2, 39.9, 39.8, 35.7, 34.0, 34.0, 33.2, 33.1, 27.3, 27.0, 24.6, 20.0, 15.8. HRMS (ESI) calcd for C.sub.36H.sub.49ClN.sub.3O.sub.9S 734.2873 [M+H].sup.+, found 734.2871.

Example 19

[0131] ##STR00029##

[0132] 4-((4-((4-((1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-3-yl)methoxyl)-4-oxobutanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 3, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 3-(hydroxymethyl)piperidine.

[0133] In this example, a yellow waxy liquid was obtained, with a yield of 70%.

[0134] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.5 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.8, 10.4 Hz, 1H), 5.25 (s, 1H), 5.10 (s, 2H), 4.91 (d, J=14.0 Hz, 5H), 4.76 (s, 2H), 4.11-3.91 (m, 4H), 2.88 (s, 2H), 2.80-2.51 (m, J=3.7 Hz, 7H), 2.31-2.21 (m, 1H), 2.12 (s, 1H), 1.92 (s, 3H), 1.71-1.39 (m, 10H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.2, 171.6, 158.0, 149.4, 147.8, 137.9, 135.9, 129.8, 128.8, 116.2, 111.3, 110.7, 83.9, 78.7, 67.7, 63.9, 58.7, 57.0, 54.2, 52.2, 51.1, 47.8, 42.2, 39.9, 39.8, 35.7, 34.0, 29.0, 28.9, 27.3, 27.0, 24.6, 15.8. HRMS (ESI) calcd for C.sub.37H.sub.47ClN.sub.3O.sub.9S 744.2716 [M+H].sup.+, found 744.2709.

Example 20

[0135] ##STR00030##

[0136] 4-((4-((5-((1-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperidin-3-yl)methoxyl)-5-oxopentanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 4, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with 3-(hydroxymethyl)piperidine.

[0137] In this example, a yellow waxy liquid was obtained, with a yield of 73%.

[0138] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.7 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.9, 10.4 Hz, 1H), 5.26 (s, 1H), 5.10 (s, 2H), 4.92 (d, J=14.0 Hz, 5H), 4.74 (s, 2H), 4.09 (d, J=11.7 Hz, 1H), 3.94 (dd, J=18.4, 11.3 Hz, 3H), 3.02-2.57 (m, 5H), 2.40 (dt, J=20.6, 7.3 Hz, 5H), 2.31-2.23 (m, 1H), 2.11 (s, 1H), 1.96 (p, J=7.3 Hz, 4H), 1.70-1.42 (m, 10H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.9, 172.2, 158.0, 151.1, 149.4, 147.8, 138.0, 135.9, 129.8, 128.8, 116.2, 111.3, 110.7, 84.1, 78.7, 67.4, 64.0, 58.7, 57.0, 54.2, 52.0, 51.1, 47.9, 42.3, 39.9, 39.9, 35.8, 34.1, 33.2, 33.1, 27.3, 27.1, 24.7, 20.1, 15.9. HRMS (ESI) calcd for C.sub.38H.sub.49ClN.sub.3O.sub.9S 758.2873 [M+H].sup.+, found 758.2897.

Example 21

[0139] ##STR00031##

[0140] 4-(2-((4-(2-(4-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperazin-1-yl)ethyoxyl)-4-oxobutanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 1, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with N-(hydroxyethyl)piperazine.

[0141] In this example, a yellow waxy liquid was obtained, with a yield of 53%.

[0142] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=7.3 Hz, 2H), 7.75 (t, J=7.5 Hz, 1H), 7.62 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.8, 10.4 Hz, 1H), 5.26 (s, 1H), 4.98-4.83 (m, 5H), 4.62 (dd, J=5.6, 3.5 Hz, 2H), 4.51 (dd, J=5.4, 3.6 Hz, 2H), 4.21 (t, J=6.0 Hz, 2H), 4.08 (d, J=11.6 Hz, 1H), 3.96 (d, J=11.7 Hz, 1H), 2.97-2.84 (m, 2H), 2.67 (s, 4H), 2.63 (t, J=6.0 Hz, 2H), 2.46 (d, J=40.2 Hz, 8H), 2.29-2.24 (m, 1H), 2.15-2.08 (m, 1H), 1.64-1.43 (m, 6H), 0.97 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.2, 172.1, 158.8, 150.7, 149.4, 147.9, 138.3, 135.7, 129.8, 128.8, 116.2, 111.2, 111.0, 110.5, 69.0, 63.5, 62.4, 61.5, 56.7, 53.7, 53.2, 51.0, 48.0, 42.4, 39.9, 34.1, 29.1, 29.1, 27.1, 15.9. HRMS (ESI) calcd for C.sub.35H.sub.48ClN.sub.4O.sub.9S 735.2825 [M+H].sup.+, found 735.2831.

Example 22

[0143] ##STR00032##

[0144] 4-(2-((4-(2-(4-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperazin-1-yl)ethyoxyl)-4-oxobutanoyl)oxy)ethyoxyl)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 2, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with N-(hydroxyethyl)piperazine.

[0145] In this example, a yellow waxy liquid was obtained, with a yield of 56%.

[0146] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=7.3 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.62 (t, J=7.9 Hz, 2H), 5.78 (dd, J=17.9, 10.4 Hz, 1H), 5.26 (s, 1H), 4.99-4.86 (m, 5H), 4.65-4.61 (m, 2H), 4.53-4.47 (m, 2H), 4.21 (t, J=5.9 Hz, 2H), 4.09 (d, J=11.1 Hz, 1H), 3.97 (d, J=11.7 Hz, 1H), 2.92 (s, 2H), 2.62 (t, J=5.9 Hz, 2H), 2.59-2.36 (m, 12H), 2.30-2.24 (m, 1H), 2.16-2.09 (m, 1H), 2.01-1.95 (m, 2H), 1.81 (s, 2H), 1.64-1.41 (m, 6H), 0.98 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.9, 172.7, 158.8, 150.7, 149.4, 147.9, 138.3, 135.8, 129.8, 128.8, 116.2, 111.3, 111.0, 110.6, 69.1, 63.5, 62.0, 61.3, 56.8, 53.6, 53.2, 51.1, 48.0, 42.4, 40.0, 34.1, 33.3, 33.2, 29.8, 27.2, 20.1, 15.9. HRMS (ESI) calcd for C.sub.36H.sub.50ClN.sub.4O.sub.9S 749.2982 [M+H].sup.+, found 749.2993.

Example 23

[0147] ##STR00033##

[0148] 4-((4-((4-(2-(4-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperazin-1-yl)ethyoxyl)-4-oxobutanoyl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 3, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with N-(hydroxyethyl)piperazine.

[0149] In this example, a yellow waxy liquid was obtained, with a yield of 60%.

[0150] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.07 (d, J=7.5 Hz, 2H), 7.77 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.8 Hz, 2H), 5.78 (dd, J=17.8, 10.4 Hz, 1H), 5.26 (s, 1H), 5.10 (s, 2H), 4.98-4.86 (m, 5H), 4.76 (s, 2H), 4.23 (t, J=5.9 Hz, 2H), 4.09 (d, J=11.6 Hz, 1H), 3.97 (d, J=11.7 Hz, 1H), 2.92 (s, 2H), 2.69-2.62 (m, 6H), 2.48 (d, J=40.6 Hz, 7H), 2.30-2.24 (m, 1H), 2.16-2.08 (m, 1H), 1.89 (s, 1H), 1.64-1.43 (m, 6H), 0.98 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.1, 171.6, 158.1, 150.7, 149.4, 147.9, 138.1, 135.8, 129.8, 128.8, 116.2, 111.3, 111.1, 110.7, 84.0, 78.8, 63.5, 62.4, 58.7, 56.7, 53.6, 53.2, 52.2, 51.1, 48.0, 42.4, 40.0, 34.1, 29.1, 29.0, 27.2, 15.9. HRMS (ESI) calcd for C.sub.37H.sub.48ClN.sub.4O.sub.9S 759.2825 [M+H].sup.+, found 759.2811.

Example 24

[0151] ##STR00034##

[0152] 4-((4-((5-(2-(4-(2-((1R,3R,4S)-3-(3-chloroprop-1-en-2-yl)-4-methyl-4-vinyl cyclohexyl)allyl)piperazin-1-yl)ethyoxyl)-5-oxyglutaryl)oxy)but-2-yn-1-yl)oxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole 2-oxide was prepared basically according to the procedures as described in Example 4, except that: N-methyl-2-hydroxyethylamine used in (1) was replaced with N-(hydroxyethyl)piperazine.

[0153] In this example, a yellow waxy liquid was obtained, with a yield of 58%.

[0154] .sup.1H NMR (400 MHz, CDCl.sub.3) ? 8.06 (d, J=7.5 Hz, 2H), 7.76 (t, J=7.5 Hz, 1H), 7.63 (t, J=7.9 Hz, 2H), 5.77 (dd, J=17.5, 10.7 Hz, 1H), 5.25 (s, 1H), 5.09 (s, 2H), 4.96-4.86 (m, 5H), 4.73 (s, 2H), 4.20 (t, J=5.9 Hz, 2H), 4.08 (d, J=11.5 Hz, 1H), 3.96 (d, J=11.7 Hz, 1H), 2.91 (s, 2H), 2.62 (t, J=5.9 Hz, 2H), 2.42 (dt, J=20.1, 7.3 Hz, 12H), 2.29-2.24 (m, 1H), 2.13-2.07 (m, 1H), 1.98-1.91 (m, 2H), 1.63-1.41 (m, 6H), 0.96 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) ? 172.8, 172.1, 157.9, 150.5, 149.3, 147.7, 137.8, 135.8, 129.8, 128.7, 116.2, 111.2, 111.0, 110.6, 84.0, 78.6, 63.3, 61.8, 58.6, 56.7, 53.5, 53.0, 51.9, 51.1, 47.6, 42.1, 39.8, 39.7, 33.9, 33.1, 32.9, 27.0, 19.9, 15.7. HRMS (ESI) calcd for C.sub.38H.sub.50ClN.sub.4O.sub.9S 773.2982 [M+H].sup.+, found 773.2974.

Pharmacodynamic Experiment

1. In Vitro NO Release Test

1.1 Experimental Equipment and Reagents

Instruments

[0155] Multifunctional enzyme marker (MD Spectramac M3, USA) [0156] Clean bench (Suzhou Purification, China)

Reagents

[0157] Potassium dihydrogen phosphate (Aladdin Chemical Reagent Co., Ltd.) [0158] Dipotassium hydrogen phosphate (Aladdin Chemical Reagent Co., Ltd.) [0159] L-cysteine (Aladdin Chemical Reagent Co., Ltd.)

1.2 Experimental Method

[0160] (1) Sodium nitrite solutions with different concentrations were prepared. A calibration curve was drawn. [0161] (2) The sample was treated. A 100 ?M solution was prepared. 2.5 mL of the prepared compound solution and 2.5 mL of the cysteine solution were taken and incubated at 37? C. for 120 min, and the resulting mixture was sampled at different time points. [0162] (3) A blank hole and a standard hole were set. 160 ?L of the sample was collected and mixed evenly with 0.08 mL of a developer and stood at 37? C. for 15 min. At ?=550 nm, the OD value of absorbance was measured by the enzyme marker. The NO release levels were obtained by substituting the value into the standard curve.

1.3 Test Results

[0163]

TABLE-US-00001 TABLE 1 Results of the in vitro NO release from ?-elemene derivatives prepared in Examples 1-24 NO-release concentrations (?M) Example 5 min 10 min 20 min 30 min 60 min 90 min 120 min Example 1 1.73 2.31 2.90 4.04 7.76 12.67 17.21 Example 2 2.10 2.40 2.84 3.79 6.74 10.72 14.97 Example 3 10.78 12.02 15.83 19.79 32.99 48.39 58.84 Example 4 12.53 14.80 19.55 23.6 42.31 66.97 93.18 Example 5 0.59 41.61 48.58 2.57 5.58 9.22 13.64 Example 6 3.38 5.87 4.62 6.22 9.97 15.53 20.60 Example 7 5.68 7.17 9.55 9.04 20.74 30.04 40.44 Example 8 10.55 12.34 15.52 18.95 29.50 43.00 55.33 Example 9 3.25 3.90 5.98 7.59 11.11 17.29 23.48 Example 10 8.39 9.44 10.65 12.22 18.15 26.49 34.97 Example 11 9.49 10.79 14.43 15.99 24.58 37.37 50.99 Example 12 13.20 16.04 21.29 26.90 46.58 74.45 103.92 Example 13 11.09 12.18 14.69 17.13 25.80 38.78 51.94 Example 14 12.34 13.53 15.94 18.96 29.94 45.42 62.49 Example 15 8.67 10.07 12.979 15.92 25.47 36.31 48.15 Example 16 16.99 18.93 22.62 27.04 42.47 64.24 86.29 Example 17 4.84 5.66 6.95 8.44 12.43 17.81 22.91 Example 18 8.89 10.18 12.42 14.32 21.50 32.40 42.83 Example 19 13.76 15.87 19.21 22.88 34.60 48.73 56.52 Example 20 15.19 17.57 21.33 25.52 39.29 58.12 77.28 Example 21 0.08 0.41 0.99 1.64 2.98 6.43 10.20 Example 22 0.33 0.90 1.35 1.91 4.05 7.20 10.51 Example 23 4.74 5.95 7.49 7.63 8.44 13.57 17.82 Example 24 10.64 11.37 12.38 13.68 18.24 25.90 34.64

[0164] As can be seen from the data in Table 1, all compounds prepared in the examples could effectively release NO, and in most of examples, the level of NO-release increases with increasing time.

2. In Vitro Anti-Tumor Activity Evaluation Test

2.1 Experimental Equipment and Reagents

Instruments:

[0165] Clean bench (Suzhou Purification Equipment Co., Ltd.) [0166] CO.sub.2 incubator (SANYO, Japan) [0167] Inverted biological microscope (OLYMPUS, Japan) [0168] Enzyme marker (BioTek, USA)

Reagents:

[0169] Penicillin and streptomycin mixture (Jiangsu KeyGEN Biotechnology Co., Ltd., China) [0170] Trypsin digestion solution (Jiangsu KeyGEN Biotechnology Co., Ltd., China) [0171] PBS (Jiangsu KeyGEN Biotechnology Co., Ltd., China) [0172] Fetal Bovine Serum (GIBCO) [0173] RPMI-1640 (Jiangsu KeyGEN Biotechnology Co., Ltd., China) [0174] DMEM (Jiangsu KeyGEN Biotechnology Co., Ltd., China) [0175] L-15 (Jiangsu KeyGEN Biotechnology Co., Ltd., China) [0176] CCK8 (Jiangsu KeyGEN Biotechnology Co., Ltd., China) [0177] DMSO (SIGMA)

2.2 Experimental Method

[0178] (1) Cells were digested and counted. Cell suspensions (H520, SW620 5.0?104 cells/mL, and U87MG 3.5?104 cells/mL) were prepared. 100 ?L of cell suspension was added to each well of a 96-well cell culture plate. [0179] (2) The 96-well cell culture plate was incubated in a 37? C., 5%, CO.sub.2 incubator for 24 h. [0180] (3) A drug was diluted with a culture medium to a desired working solution concentration. 100 ?L of the corresponding drug-containing culture medium was added to each well. At the same time, a negative control group and a positive control group were set up. [0181] (4) The 96-well cell culture plate was incubated in a 37? C., 5%, CO.sub.2 incubator for 72 h. [0182] (5) The 96-well plate was stained with CCK-8, and at ?=450 nm, the OD value was determined. [0183] 1) 10 ?L of CCK-8 was added to each well. The culture plate continued to be incubated in the incubator for 2-3 h. [0184] 2) The culture plate was rocked for 10 min to gently mix and remove air bubbles from the 96-well plate. [0185] 3) At ?=450 nm, the OD value of each well was read by the enzyme marker, and the inhibitory rates were calculated. [0186] (6) According to the following equation (1), the growth inhibitory rate of the tumor cells was obtained. The obtained results were substituted into an IC 50 calculation software SPSS17.0 to find out the IC50 value. The IC50 results are shown in Table 1.

[00001]$\begin{array}{cc}\mathrm{Cell}\mathrm{inhibitory}\mathrm{rate}\%=\frac{\begin{array}{c}\mathrm{the}\mathrm{OD}\mathrm{value}\mathrm{of}\mathrm{the}\mathrm{negative}\mathrm{group}-\\ \mathrm{the}\mathrm{OD}\mathrm{value}\mathrm{of}\mathrm{the}\mathrm{experimental}\mathrm{group}\end{array}}{\mathrm{the}\mathrm{OD}\mathrm{value}\mathrm{of}\mathrm{the}\mathrm{negative}\mathrm{group}}?100\%& \mathrm{Equation}\left(1\right)\end{array}$

2.3 Experimental Results

[0187]

TABLE-US-00002 TABLE 2 Inhibitory rates of compounds prepared in Examples 1-24 (1 ?M) against three human tumor cell lines in terms of antiproliferative activity Inhibitory rates of compounds prepared in examples against different tumor cell lines (%) Examples SW620 U87MG NCI-H520 ?-elemene 2.5 ? 4.1 4.3 ? 2.2 3.2 ? 0.7 13,14-dichloro-?- 3.3 ? 1.0 3.5 ? 1.0 0.9 ? 3.0 elemene Example 1 30.7 ? 3.5 39.5 ? 2.1 28.8 ? 0.8 Example 2 42.3 ? 3.7 56.0 ? 2.1 48.5 ? 3.0 Example 3 30.1 ? 5.2 34.9 ? 0.6 35.0 ? 2.2 Example 4 47.5 ? 3.7 55.5 ? 1.9 48.7 ? 2.0 Example 5 33.0 ? 2.5 47.1 ? 0.9 44.2 ? 1.3 Example 6 35.6 ? 2.8 54.3 ? 1.3 43.4 ? 1.5 Example 7 46.6 ? 1.2 56.7 ? 0.9 44.5 ? 2.2 Example 8 10.8 ? 3.2 4.5 ? 1.0 5.6 ? 1.6 Example 9 39.3 ? 4.2 51.3 ? 1.3 44.5 ? 2.8 Example 10 45.9 ? 3.9 53.1 ? 1.4 46.7 ? 2.0 Example 11 30.5 ? 3.3 42.0 ? 6.5 46.0 ? 1.0 Example 12 31.7 ? 1.4 47.8 ? 1.1 39.1 ? 0.7 Example 13 53.6 ? 2.4 52.6 ? 1.7 48.5 ? 2.5 Example 14 55.4 ? 1.5 54.8 ? 1.9 50.1 ? 1.1 Example 15 31.3 ? 0.3 40.5 ? 3.3 45.9 ? 1.4 Example 16 39.8 ? 1.9 52.2 ? 3.4 52.3 ? 1.1 Example 17 38.3 ? 3.0 51.0 ? 2.2 45.4 ? 1.9 Example 18 46.1 ? 0.9 48.3 ? 1.5 43.9 ? 1.0 Example 19 40.6 ? 2.8 41.9 ? 1.1 46.1 ? 2.7 Example 20 43.2 ? 1.8 53.9 ? 0.6 44.5 ? 2.4 Example 21 43.6 ? 2.5 52.6 ? 1.0 47.3 ? 2.6 Example 22 42.8 ? 0.5 52.4 ? 2.0 44.1 ? 2.4 Example 23 21.2 ? 2.1 33.4 ? 1.5 30.3 ? 2.9 Example 24 43.2 ? 5.5 50.3 ? 1.2 46.8 ? 2.1

[0188] As can be seen from Table 2, at the concentration of 1 ?M, all 24 compounds exhibit different degrees of inhibition effect against the three human tumor cells SW620, U87MG, and NCI-H520. The antiproliferative activities are significantly stronger than ?-elemene and 13,14-dichloro-?-elemene, indicating that the introduction of NO donors has successfully improved the anti-tumor effect of ?-elemene.

TABLE-US-00003 TABLE 3 IC.sub.50 (?M) of the compounds prepared in the preferred examples against three human tumor cell lines in terms of antiproliferative activities IC.sub.50 against different tumor cell lines (?M) Example SW620 U87MG NCI-H520 ?-elemene >100 >100 >100 13,14-dichloro-?- >100 >100 >100 elemene Example 2 0.358 ? 0.023 0.777 ? 0.024 Example 4 0.858 ? 0.033 0.369 ? 0.013 0.719 ? 0.017 Example 5 0.369 ? 0.025 Example 6 0.366 ? 0.019 Example 7 1.111 ? 0.115 0.882 ? 0.034 Example 9 0.323 ? 0.013 Example 10 1.022 ? 0.09 0.343 ? 0.029 0.848 ? 0.053 Example 11 1.019 ? 0.041 Example 12 0.898 ? 0.054 Example 13 1.069 ? 0.101 1.119 ? 0.022 1.301 ? 0.043 Example 14 0.814 ? 0.068 0.477 ? 0.011 0.846 ? 0.027 Example 15 1.038 ? 0.033 Example 16 2.160 ? 0.548 1.046 ? 0.013 Example 17 1.088 ? 0.04 0.811 ? 0.016 Example 18 1.212 ? 0.152 Example 19 0.985 ? 0.012 Example 20 1.114 ? 0.052 1.175 ? 0.066 Example 21 0.926 ? 0.052 Example 22 0.372 ? 0.006 0.962 ? 0.054 Example 24 0.960 ? 0.075 1.062 ? 0.002

[0189] As shown in Table 3, the antiproliferative activities of most of compounds are significantly more potent than ?-elemene and 13,14-dichloro-?-elemene. For SW620 cell line, the activities of Examples 4 and 14 are over 110-fold more active than ?-elemene; for U87MG cell line, the antiproliferative activities of Examples 2, 4, 5, 6, 9, 10, and 22 are over 250-fold more potent than ?-elemene; and for NCI-H520 cell line, the activities of Examples 2, 4, 10, 14, and 22 are over 100-fold more greater than ?-elemene.

3. In Vivo Anti-Tumor Activity Against Malignant Brain Glioma

3.1 Subject Animals and Experimental Equipment

Subject Animals:

[0190] Source, germ line, strain: BABLc/nude mice, provided by Shanghai Slaughter Laboratory Animal Co., Ltd., China. [0191] Experimental animal production license: SOCK (Shanghai) 2017-0005 [0192] Certificate of Conformity No.: 20170005040021 [0193] Experimental animal use license: SYXK (Su) 2017-0040 [0194] Age: 4-5 weeks [0195] Sex: female [0196] Number of animals: 5 animals per group, 15 animals in total

Experimental Instrument:

[0197] Small animal in vivo 3D imaging system Spectrum, PerkinElmer

3.2 Preparation of a Model

[0198] The cultured human brain glioma U87MG-LUC cell suspension with a concentration of 5?10 7 cells/mL was collected and each animal was inoculated in situ with 20 ?L of the cell suspension. 10% chloral hydrate was injected intraperitoneally to anesthetize the mice, and the heads of the mice were fixed with a stereotaxic instrument. The skin of the head was disinfected with an alcohol swab. The skin of the head was cut along the direction of midline of the brain at its slightly right. The right hemisphere was taken as the reference point for fontanelle, 2 mm to the right of the midline, and 1 mm in front as the entry point, and the skull was ground open with a micro-abrasive drill. The cells were fully resuspended with a gun head, a syringe was used to extract the cell suspension. A vertical needle fixed by a stereotaxic instrument was used to feed 3.5 mm and quit 0.5 mm, then stood for 1 min and slowly injected the cells. After injection, the needle was stood for 1 min and slowly withdrawn. Alcohol swabs were used to disinfect the skin, and mice were put back into the feeding cages to wake up naturally after suturing the skin with stitches.

3.3 Grouping and Administration

[0199] After 14 days of cell inoculation, the animals were randomly grouped, with 5 animals in each group. At the same time, each group of nude mice began to administer drugs. The drug administration protocol is shown in the group and drug administration protocol. At the end of the experiment, the nude mice were executed immediately. The brain tissues were surgically stripped, photographed, and weighed.

3.4 In Vivo Imaging Detection

[0200] Two animals in each group were selected and injected intraperitoneally with Luc bioluminescence signal substrate, 100 ?L/each animal, before administration, 1 week, 2 weeks, and 3 weeks after drug administration, respectively. 10 min later, the mice were anesthetized with isoflurane and placed on black box loading platform of an instrument for in vivo imaging detection to observe the bioluminescence signal of in situ tumors in mice's brains.

3.5 Experimental Results

[0201] As shown in FIG. 6, the growth of malignant brain gliomas in mice is effectively inhibited after the administration of ?-elemene and Example 4 for 3 weeks. The tumor volume in the model group continues to increase (FIG. 6), but the tumor volume continues to be inhibited and significantly smaller than that in the model group after treatment with ?-elemene and Example 4. In terms of brain weight, the weight of the ?-elemene and Example 4 group is significantly lighter than that of the model group, and the living and physical conditions of mice are also much better than the model group. On the other hand, the bioluminescence signal intensity of gliomas in the model group continues to increase rapidly, but the bioluminescence signal intensity of ?-elemene and Example 4 group declines dramatically. In the first week, the inhibitory activity of Example 4 exceeds 80%, which is significantly more potent than that of ?-elemene (>20%). In the second week, both ?-elemene and Example 4 reaches >80% inhibition. In the last week of administration, Example 4 blocks the tumor growth by >90%, exhibiting potent anti-malignant brain glioma activity. In summary, both ?-elemene and Example 4 exhibits effective therapeutic activity, but Example 4 exhibits stronger inhibition than that of ?-elemene.

[0202] The above results indicate that the introduction of NO donors effectively enhances the anti-tumor effect of ?-elemene in vivo, and is a feasible strategy for the development of ?-elemene based antitumor drugs.

[0203] Furthermore, it should be understood that after reading the above described contents of the present disclosure, various improvements or modifications can be made to the present disclosure by those skilled in the art, and these equivalent forms likewise fall within the scope limited by the claims appended to the present disclosure.