METHOD FOR EXTRACTION AND MULTI-SCENARIO UTILIZATION OF FLAXSEED PROTEIN-POLYSACCHARIDE NATURAL MIXTURE

Abstract

A method for extraction and multi-scenario utilization of flaxseed protein-polysaccharide natural mixture is disclosed, comprising:(1) raw material pretreatment; (2) extraction of mixture I; (3) multi-scenario utilization of mixture I; (4) extraction of mixture II; and (5) multi-scenario utilization of mixture II. Compared with the traditional extraction mode (such as alkali-solution and acid-isolation method) of flaxseed protein, the method of the invention can enrich the flaxseed protein more efficiently, greenly and moderately, and can co-extract polysaccharide from flaxseed meal to form the flaxseed protein-polysaccharide natural mixture. Physical field/bio-enzyme coupling ultrasonic technique solves the bottleneck problem of low extraction rate of the flaxseed protein-polysaccharide natural mixture by water extraction method. By controlling the types of physical field/enzyme, action parameters and treatment time, flaxseed protein-polysaccharide natural mixtures with different proportions can be customized and endowed with multiple functional properties, so as to satisfy the multi-scenario utilization of mixtures I and II.

Claims

1. A method for extraction and multi-scenario utilization of a flaxseed protein-polysaccharide natural mixture, specifically comprising the following steps: (1) raw material pretreatment firstly crushing, sieving and degreasing flaxseed meal to obtain flaxseed degreased meal powder; and then dissolving the flaxseed degreased meal in water and stirring to obtain flaxseed degreased meal rinsing solution; (2) extraction of flaxseed protein-polysaccharide natural mixture I firstly conducting ultrasonic treatment for the flaxseed degreased meal rinsing solution, or firstly conducting microwave treatment for the flaxseed degreased meal rinsing solution, or firstly adding bio-enzyme into the flaxseed degreased meal rinsing solution and stirring in a water bath, wherein the bio-enzyme is pectase, cellulase or hemicellulase; then centrifuging, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture I; (3) utilization of flaxseed protein-polysaccharide natural mixture I firstly, dissolving the flaxseed protein-polysaccharide natural mixture I prepared by ultrasonic treatment in above step (2), gum Arabic and maltodextrin in water and hydrating overnight; then adding oil rich in n-3 polyunsaturated fatty acid, shearing, introducing a high-pressure microfluidization, adding food grade silica powder and shaking up to obtain emulsion rich in n-3 polyunsaturated fatty acid; and finally, spray-drying the emulsion to obtain powdered oil rich in n-3 polyunsaturated fatty acid; or, firstly dissolving the flaxseed protein-polysaccharide natural mixture I prepared by microwave treatment in above step (2) in water and adjusting pH to acidity; then heating in the water bath and whipping; and finally, treating in an ice bath to room temperature to obtain high-stability gel foam; or, using the flaxseed protein-polysaccharide natural mixture I prepared by the bio-enzyme in above step (2) as a highly soluble protein ingredient for preparing a low-turbidity plant protein drink; (4) extraction of flaxseed protein-polysaccharide natural mixture II firstly, redissolving the precipitate obtained after centrifugation in step (2) to the same volume as the flaxseed degreased meal rinsing solution, adjusting the pH to alkalinity and conducting magnetic stirring to obtain the alkali extraction solution of the flaxseed degreased meal; then, conducting ultrasonic treatment for the alkali extraction solution of the flaxseed degreased meal, centrifuging, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture II; (5) utilization of flaxseed protein-polysaccharide natural mixture II firstly dissolving the flaxseed protein-polysaccharide natural mixture II in water, then adding the oil rich in n-3 polyunsaturated fatty acid, and after shearing, introducing the high-pressure microfluidization to obtain low oil phase nanoemulsion; or, firstly dissolving the flaxseed protein-polysaccharide natural mixture II and sucrose in water, adding coconut oil, shearing to obtain macroemulsion, introducing the high-pressure microfluidization to obtain miniemulsion, then adding the flaxseed protein-polysaccharide natural mixture I prepared by microwave treatment in above step (2) for magnetic stirring, aging, adjusting pH to acidity, and whipping in an ice bath to obtain foamed emulsion; or, mixing and crushing the flaxseed protein-polysaccharide natural mixture II, pea protein isolate, hemp seed protein, soybean protein isolate, perilla protein, perilla peptide, microcapsule powder rich in n-3 polyunsaturated fatty acid, medium chain triglycerides microcapsule powder, psyllium husk powder, maltodextrin, oat flour, flaxseed powder, resistant dextrin, chitosan oligosaccharide, yacon, inulin, fructo oligosaccharide, erythritol, konjaku flour and stevioside to obtain a meal replacement milkshake.

2. The method for extraction and multi-scenario utilization of the flaxseed protein-polysaccharide natural mixture according to claim 1, wherein in step (1), the sieve mesh number for sieving is 60 meshes; the volume ratio of the flaxseed degreased meal to the water is 1:15; and the rotational speed of the stirring is 1600 rpm, temperature is 40? C. and time is 3h.

3. The method for extraction and multi-scenario utilization of the flaxseed protein-polysaccharide natural mixture according to claim 1, wherein in step (2), the power of the ultrasonic treatment is 1-20 W/mL and time is 10-60 min; the temperature of the microwave treatment is 60? C., power is 40-100 W and time is 3-40 min; the mass-to-volume ratio of the bio-enzyme to the flaxseed degreased meal rinsing solution is 1 g:100 mL; the temperature of the stirring in the water bath is 50? C., and time is 2 h; and the temperature of the centrifuging is 20? C., rotational speed is 10000 rpm and time is 30 min.

4. The method for extraction and multi-scenario utilization of the flaxseed protein-polysaccharide natural mixture according to claim 1, wherein in step (3), in the preparation process of the powdered oil, the mass-to-volume ratio of the flaxseed protein-polysaccharide natural mixture I, the gum Arabic, the maltodextrin, the water, the oil rich in n-3 polyunsaturated fatty acid and the food grade silica powder is 2.5 g:2.5 g:5g:100 mL: 2.55 g:0.3 g; the temperature of hydrating overnight is 4? C.; the shear rate is 15000 rpm and time is 10 min; the pressure of the high-pressure microfluidization is 750 bar and cycle times is 3; the air inlet temperature of the spray drying is 160? C., and a feed rate is 7 mL/min.

5. The method for extraction and multi-scenario utilization of the flaxseed protein-polysaccharide natural mixture according to claim 1, wherein in step (3), in the preparation process of the foam, the mass-to-volume ratio of the flaxseed protein-polysaccharide natural mixture Ito the water is 2 g:50 mL; the pH is adjusted to 3.5; the temperature of the heating in the water bath is 50? C. and time is 10 min; the time of the whipping is 5 min.

6. The method for extraction and multi-scenario utilization of the flaxseed protein-polysaccharide natural mixture according to claim 1, wherein in step (4), the pH is adjusted to 9.0; the time of the magnetic stirring is 2 h; the power of the ultrasonic treatment is 1-20 W/mL and time is 10-60 min; and the temperature of the centrifuging is 4? C., rotational speed is 10000 rpm and time is 30 min.

7. The method for extraction and multi-scenario utilization of the flaxseed protein-polysaccharide natural mixture according to claim 1, wherein in step (5), in the preparation process of the low oil phase nanoemulsion, the mass-to-volume ratio of the flaxseed protein-polysaccharide natural mixture II, the water and the oil rich in n-3 polyunsaturated fatty acid is 1.5 g:100 mL: 3 g; the shear rate is 15000 rpm and time is 5 min; and the pressure of the high-pressure microfluidization is 500 bar and cycle times is 3.

8. The method for extraction and multi-scenario utilization of the flaxseed protein-polysaccharide natural mixture according to claim 1, wherein in step (5), in the preparation process of the foamed emulsion, the mass-to-volume ratio of the flaxseed protein-polysaccharide natural mixture II, the sucrose, the water, the coconut oil and the flaxseed protein-polysaccharide natural mixture I is 2 g:15 g:90 mL: 10 mL: 2 g; the temperature of the coconut oil is 60? C.; the shear rate is 15000 rpm and time is 5 min; the pressure of the high-pressure microfluidization is 500 bar and cycle times is 2; the time of the magnetic stirring is 6 h; the temperature of the aging is 4? C., and time is 6 h; the pH is adjusted to 4.0; and the time of the whipping is 20 min.

9. The method for extraction and multi-scenario utilization of the flaxseed protein-polysaccharide natural mixture according to claim 1, wherein in step (5), in the preparation process of the meal replacement milkshake, the weight parts of the raw materials are: 2 parts of the flaxseed protein-polysaccharide natural mixture II, 9 parts of the pea protein isolate, 3 parts of the hemp seed protein, 7 parts of the soybean protein isolate, 5 parts of the perilla protein, 3 parts of the perilla peptide, 5 parts of the microcapsule powder rich in n-3 polyunsaturated fatty acid, 3 parts of the medium chain triglycerides microcapsule powder, 2 parts of the psyllium husk powder, 6 parts of the maltodextrin, 1 part of the oat flour, 1 part of the flaxseed powder, 5 parts of the resistant dextrin, 0.1 part of the chitosan oligosaccharide, 0.5 part of the yacon, 1 part of the inulin, 1 part of the fructo oligosaccharide, 1 part of the erythritol, 2 parts of the konjaku flour and 0.04 part of the stevioside.

Description

DESCRIPTION OF DRAWINGS

[0050] FIG. 1 is a flow chart of a method for extraction and multi-scenario utilization of flaxseed protein-polysaccharide natural mixture in embodiments 1-27;

[0051] FIG. 2 is a flow chart of extraction of flaxseed protein-polysaccharide natural mixture without physical field/bio-enzyme coupling;

[0052] FIG. 3 shows powdered oil prepared in step (3) of embodiment 4;

[0053] FIG. 4 shows low oil phase nanoemulsion prepared in step (5) of embodiment 4;

[0054] FIG. 5 shows high-stability gel foam prepared in step (3) of embodiment 13;

[0055] FIG. 6 Shows foamed emulsion prepared in step (5) of embodiment 14;

[0056] FIG. 7 shows a low-turbidity plant protein drink prepared in step (3) of embodiment 19;

[0057] FIG. 8 shows meal replacement milkshake prepared in step (5) of embodiment 22.

DETAILED DESCRIPTION

[0058] The technical solutions in embodiments of the present invention will be clearly and fully described below. Apparently, the described embodiments are merely part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those ordinary skilled in the art without contributing creative labor will belong to the protection scope of the present invention.

[0059] In the following embodiments, the variety of flaxseed is flaxseed yellow seed Zhangya 2#.

Embodiment 1

[0060] A method for extraction and multi-scenario utilization of a flaxseed protein-polysaccharide natural mixture, as shown in FIG. 1, specifically comprises the following steps: [0061] (1) raw material pretreatment [0062] firstly crushing flaxseed meal, then sieving by a 60-mesh sieve and degreasing to obtain flaxseed degreased meal powder; and then dissolving the flaxseed degreased meal in water at a volume ratio of 1:15 and stirring in a water bath of 40? C. at 1600 rpm for 3 h to obtain flaxseed degreased meal rinsing solution; [0063] (2) extraction of flaxseed protein-polysaccharide natural mixture I [0064] firstly, conducting ultrasonic treatment for the flaxseed degreased meal rinsing solution by an ultrasonic cell crusher; setting ultrasonic power as 1 W/mL and ultrasonic time as 60 min; at the same time, to avoid solution overheating in the ultrasonic process, keeping solution temperature at 25? C. by an ice bath; then centrifuging at 10000 rpm at 20? C. for 30 min, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture I; [0065] (3) ) utilization of flaxseed protein-polysaccharide natural mixture I [0066] firstly, dissolving 2.5 g of flaxseed protein-polysaccharide natural mixture I, 2.5 g of gum Arabic and 5 g of maltodextrin in 100 mL of water and hydrating overnight at 4? C.;

[0067] then adding 2.5 g of flaxseed oil, shearing by a high-speed disperser for 10 min at 15000 rpm, then introducing a high-pressure microfluidization with a pressure of 750 bar for three cycles, adding 0.3 g of food grade silica powder and shaking up to obtain flaxseed oil emulsion; finally, introducing the flaxseed oil emulsion into a spray dryer for spray drying, setting air inlet temperature as 160? C. and a feed rate as 7 mL/min; and collecting products in a cyclone separator and a collector to obtain powdered oil; [0068] (4) extraction of flaxseed protein-polysaccharide natural mixture II [0069] firstly, redissolving the precipitate obtained after centrifugation in step (2) to the same volume as the flaxseed degreased meal rinsing solution, adjusting the pH to 9.0 and conducting magnetic stirring for 2 h; because the pH of the solution is decreased with the dissolution of the substance, the pH is adjusted every 0.5 h in the magnetic stirring process, and the alkali extraction solution of the flaxseed degreased meal is obtained; then, conducting ultrasonic treatment for the alkali extraction solution of the flaxseed degreased meal by an ultrasonic cell crusher; setting ultrasonic power as 1 W/mL and ultrasonic time as 60 min; at the same time, to avoid solution overheating in the ultrasonic process, keeping solution temperature at 25? C. by an ice bath; finally centrifuging at 10000 rpm at 4? C. for 30 min, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture II; [0070] (5) utilization of flaxseed protein-polysaccharide natural mixture II [0071] firstly dissolving 1.5 g of flaxseed protein-polysaccharide natural mixture II in 100 mL of water, then adding 3 g of flaxseed oil, shearing by a high-speed disperser for 5 min at 15000 rpm, and then introducing a high-pressure microfluidization with a pressure of 500 bar for three cycles to obtain the nanoemulsion with low oil phase.

Embodiment 2

[0072] The differences from embodiment 1 are only that in step (1), the ultrasonic power is 1 W/mL and the ultrasonic time is 60 min, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 3

[0073] The differences from embodiment 1 are only that in step (1), the ultrasonic power is 1 W/mL and the ultrasonic time is 60 min, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Embodiment 4

[0074] The differences from embodiment 1 are only that in step (1), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min, and in step (4), the ultrasonic power is 1 W/mL and the ultrasonic time is 60 min.

Embodiment 5

[0075] The differences from embodiment 1 are only that in step (1), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 6

[0076] The differences from embodiment 1 are only that in step (1), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Embodiment 7

[0077] The differences from embodiment 1 are only that in step (1), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min, and in step (4), the ultrasonic power is 1 W/mL and the ultrasonic time is 60 min.

Embodiment 8

[0078] The differences from embodiment 1 are only that in step (1), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 9

[0079] The differences from embodiment 1 are only that in step (1), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Embodiment 10

[0080] A method for extraction and multi-scenario utilization of a flaxseed protein-polysaccharide natural mixture, as shown in FIG. 1, specifically comprises the following steps: [0081] (1) raw material pretreatment [0082] firstly crushing flaxseed meal, then sieving by a 60-mesh sieve and degreasing to obtain flaxseed degreased meal powder; and then dissolving the flaxseed degreased meal in water at a volume ratio of 1:15 and stirring at 1600 rpm for 3 h to obtain flaxseed degreased meal rinsing solution; [0083] (2) extraction of flaxseed protein-polysaccharide natural mixture I [0084] firstly, conducting microwave treatment for the flaxseed degreased meal rinsing solution at temperature of 60? C., power of 40 W and time for 40 min; then, centrifuging at 10000 rpm at 20? C. for 30 min, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture I; [0085] (3) utilization of flaxseed protein-polysaccharide natural mixture I [0086] firstly dissolving 2 g of flaxseed protein-polysaccharide natural mixture I in 50 mL of water and adjusting pH to 3.5; then heating in a water bath at 50? C. for 10 min, and whipping the solution by a hand-held foam maker for 5 min; and finally, treating in an ice bath to room temperature to obtain high-stability gel foam; [0087] (4) extraction of flaxseed protein-polysaccharide natural mixture II [0088] firstly, redissolving the precipitate obtained after centrifugation in step (2) to the same volume as the flaxseed degreased meal rinsing solution, adjusting the pH to 9.0 and conducting magnetic stirring for 2 h; because the pH of the solution is decreased with the dissolution of the substance, the pH is adjusted every 0.5 h in the magnetic stirring process, and the alkali extraction solution of the flaxseed degreased meal is obtained; then, conducting ultrasonic treatment for the alkali extraction solution of the flaxseed degreased meal; setting ultrasonic power as 1 W/mL and time as 60 min; at the same time, to avoid solution overheating in the ultrasonic process, keeping solution temperature at 25? C. by an ice bath; finally centrifuging at 10000 rpm at 4? C. for 30 min, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture II; [0089] (5) utilization of flaxseed protein-polysaccharide natural mixture II [0090] firstly dissolving 2 g of flaxseed protein-polysaccharide natural mixture II and 15 g of sucrose in 90 mL of water, adding 10 mL of coconut oil at 60? C., shearing by a high-speed disperser for 5 min at 15000 rpm to obtain macroemulsion, introducing a high-pressure microfluidization with pressure of 500 bar for 2 cycles to obtain miniemulsion, then adding 2 g of flaxseed protein-polysaccharide natural mixture I for magnetic stirring for 6 h, aging at 4? C. for 6 h, adjusting pH to 4.0, and whipping by the hand-held foam maker in an ice bath for 20 min to obtain foamed emulsion.

Embodiment 11

[0091] The differences from embodiment 10 are only that in step (1), the microwave power is 40 W/mL and the microwave time is 40 min, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 12

[0092] The differences from embodiment 10 are only that in step (1), the microwave power is 40 W/mL and the microwave time is 40 min, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Embodiment 13

[0093] The differences from embodiment 10 are only that in step (1), the microwave power is 50 W/mL and the microwave time is 20 min, and in step (4), the ultrasonic power is 1 W/mL and the ultrasonic time is 60 min.

Embodiment 14

[0094] The differences from embodiment 10 are only that in step (1), the microwave power is 50 W/mL and the microwave time is 20 min, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 15

[0095] The differences from embodiment 10 are only that in step (1), the microwave power is 50 W/mL and the microwave time is 20 min, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Embodiment 16

[0096] The differences from embodiment 10 are only that in step (1), the microwave power is 100 W/mL and the microwave time is 3 min, and in step (4), the ultrasonic power is 1 W/mL and the ultrasonic time is 60 min.

Embodiment 17

[0097] The differences from embodiment 10 are only that in step (1), the microwave power is 100 W/mL and the microwave time is 3 min, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 18

[0098] The differences from embodiment 10 are only that in step (1), the microwave power is 100 W/mL and the microwave time is 3 min, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Embodiment 19

[0099] A method for extraction and multi-scenario utilization of a flaxseed protein-polysaccharide natural mixture, as shown in FIG. 1, specifically comprises the following steps: [0100] (1) raw material pretreatment [0101] firstly crushing flaxseed meal, then sieving by a 60-mesh sieve and degreasing to obtain flaxseed degreased meal powder; and then dissolving the flaxseed degreased meal in water at a volume ratio of 1:15 and stirring at 1600 rpm for 3 h to obtain flaxseed degreased meal rinsing solution; [0102] (2) extraction of flaxseed protein-polysaccharide natural mixture I [0103] firstly, adding 1 g of pectase to 100 mL of flaxseed degreased meal rinsing solution and stirring in a water bath at 50? C. for 2 h; then, centrifuging at 10000 rpm at 20? C. for 30 min, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture I; [0104] (3) utilization of flaxseed protein-polysaccharide natural mixture I

[0105] using the flaxseed protein-polysaccharide natural mixture I as a highly soluble protein ingredient for preparing a low-turbidity plant protein drink; [0106] (4) extraction of flaxseed protein-polysaccharide natural mixture II [0107] firstly, redissolving the precipitate obtained after centrifugation in step (2) to the same volume as the flaxseed degreased meal rinsing solution, adjusting the pH to 9.0 and conducting magnetic stirring for 2 h; because the pH of the solution is decreased with the dissolution of the substance, the pH is adjusted every 0.5 h in the magnetic stirring process, and the alkali extraction solution of the flaxseed degreased meal is obtained; then, conducting ultrasonic treatment for the alkali extraction solution of the flaxseed degreased meal; setting ultrasonic power as 1 W/mL and time as 60 min; at the same time, to avoid solution overheating in the ultrasonic process, keeping solution temperature at 25? C. by an ice bath; finally centrifuging at 10000 rpm at 4? C. for 30 min, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture II; [0108] (5) utilization of flaxseed protein-polysaccharide natural mixture II [0109] mixing and crushing 2 g of flaxseed protein-polysaccharide natural mixture II, 9 g of pea protein isolate, 3 g of hemp seed protein, 7 g of soybean protein isolate, 5 g of perilla protein, 3 g of perilla peptide, 5 g of flaxseed oil microcapsule powder, 3 g of medium chain triglycerides microcapsule powder, 2 g of psyllium husk powder, 6 g of maltodextrin, 1 g of oat flour, 1 g of flaxseed powder, 5 g of resistant dextrin, 0.1 g of chitosan oligosaccharide, 0.5 g of yacon, 1 g of inulin, 1 g of fructo oligosaccharide, 1 g of erythritol, 2 g of konjaku powder and 0.04 g of stevioside in a crusher to obtain a meal replacement milkshake.

Embodiment 20

[0110] The differences from embodiment 19 are only that in step (1), the bio-enzyme is pectase, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 21

[0111] The differences from embodiment 19 are only that in step (1), the bio-enzyme is pectase, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Embodiment 22

[0112] The differences from embodiment 19 are only that in step (1), the bio-enzyme is cellulase, and in step (4), the ultrasonic power is 1 W/mL and the ultrasonic time is 60 min.

Embodiment 23

[0113] The differences from embodiment 19 are only that in step (1), the bio-enzyme is cellulase, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 24

[0114] The differences from embodiment 19 are only that in step (1), the bio-enzyme is cellulase, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Embodiment 25

[0115] The differences from embodiment 19 are only that in step (1), the bio-enzyme is hemicellulase, and in step (4), the ultrasonic power is 1 W/mL and the ultrasonic time is 60 min.

Embodiment 26

[0116] The differences from embodiment 19 are only that in step (1), the bio-enzyme is hemicellulase, and in step (4), the ultrasonic power is 10 W/mL and the ultrasonic time is 30 min.

Embodiment 27

[0117] The differences from embodiment 19 are only that in step (1), the bio-enzyme is hemicellulase, and in step (4), the ultrasonic power is 20 W/mL and the ultrasonic time is 10 min.

Reference Example

[0118] A method for extracting a flaxseed protein-polysaccharide natural mixture without physical field/bio-enzyme coupling, as shown in FIG. 2, specifically comprises the following steps: [0119] (1) raw material pretreatment [0120] firstly crushing flaxseed meal, then sieving by a 60-mesh sieve and degreasing to obtain flaxseed degreased meal powder; and then dissolving the flaxseed degreased meal in water at a volume ratio of 1:15 and stirring in a water bath of 40? C. at 1600 rpm for 3h to obtain flaxseed degreased meal rinsing solution; [0121] (2) extraction of flaxseed protein-polysaccharide natural mixture I

[0122] centrifuging at 10000 rpm at 20? C. for 30 min, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture I; [0123] (3) extraction of flaxseed protein-polysaccharide natural mixture II [0124] firstly, redissolving the precipitate obtained after centrifugation in step (2) to the same volume as the flaxseed degreased meal rinsing solution, adjusting the pH to 9.0 and conducting magnetic stirring for 2 h; because the pH of the solution is decreased with the dissolution of the substance, the pH is adjusted every 0.5 h in the magnetic stirring process, and the alkali extraction solution of the flaxseed degreased meal is obtained; and finally centrifuging at 10000 rpm at 4? C. for 30 min, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture II.

Performance Test

[0125] In the following tests:

[0126] Detection of extraction rate: mass percentage of the flaxseed protein-polysaccharide natural mixture and the flaxseed degreased meal.

[0127] Detection of protein content: a micro Kjeldahl method is used. Refer to the national standard GB 5009.5-2010.

[0128] Detection of total sugar content: a phenol-sulfuric acid method is used. Refer to the national standard GB/T 15672-2009.

[0129] Detection of solubility: a centrifugal tube is weighed and 20 mL 2% (w/v) flaxseed protein-polysaccharide natural mixture solution is prepared with ultrapure water. The solution is stirred magnetically for 2 h until completely dissolved, and the pH is adjusted to 7.0. Then, the solution is centrifuged (7200 g, 10 min, 4? C.), the supernatant is dumped and the centrifugal tube and the precipitate are merged and dried to constant weight. The solubility is calculated.

[0130] Detection of emulsifying activity index and emulsifying stability index: 15 mL of 1% (w/v) protein solution is prepared with ultrapure water, and the pH is adjusted to 7. 5 mL of medium chain triglycerides is added and homogenized for 2 min with a high speed shearing machine (13400 r/min). 50 ?L of emulsion is quickly absorbed in the middle and diluted with 5 mL of 0.1% SDS (pH 7.0). SDS solution is used as blank reference, and the absorbance value of the diluted emulsion is determined at 500 nm. After standing for 10 min, the absorbance is determined again under the same conditions as above. The emulsifying activity index and the emulsifying stability index are calculated according to the following formulas:

[00001]$\mathrm{Emulsifying}\mathrm{activity}\mathrm{index}\left(m^2/g\right)=\frac{2?2.303?A_0?N}{c???L?10000};$$\mathrm{Emulsifying}\mathrm{stability}\mathrm{index}=\frac{A_0}{A_0-A_{10}}?t;$

[0131] In the formulas: N-dilution factor; c-protein concentration (g/mL); co-volume fraction of the oil phase; L-cuvette optical path; Ao-absorbance value at 500 nm at 0 min; A/o-absorbance value at 500 nm at 10 min; and t represents standing time.

[0132] Detection of foaming capacity and foaming stability: 15 mL of 1% (w/v) protein solution is prepared with ultrapure water, and the pH is adjusted to 7.0. The protein solution is shear for 2 min with a high speed disperser (13600 rpm). The protein solution and foam are immediately poured into a measuring cylinder and the foam volume is read out. After standing for 30 min and 60 min, the foam volume is read out again. The calculation formulas of the foaming capacity and the foaming stability are as follows:

[00002]$\mathrm{Foaming}\mathrm{capacity}\left(\%\right)=\frac{V_2}{15}?100;$$\mathrm{Foaming}\mathrm{stability}\left(\%\right)=\frac{V_{60}}{V_2}?100;$

[0133] In the formulas: V.sub.2 is the foam volume at 2 min and V.sub.60 is the foam volume at 60 min. [0134] 1. Test of flaxseed protein-polysaccharide natural mixtures I in embodiments 1, 4 and 7

[0135] The flaxseed protein-polysaccharide natural mixtures I prepared in step (2) of embodiments 1, 4 and 7 were recorded as sample 1-1, sample 1-2 and sample 1-3 respectively; and the sample without ultrasonic treatment was used as control and recorded as sample 1-0. The extraction rate, protein content, total sugar content, protein and total sugar content, solubility, emulsifying activity index, emulsifying stability index, foaming capacity and foaming stability were tested respectively, and the results were shown in Table 1.

TABLE-US-00001 TABLE 1 Test Results of Samples 1-0, 1-1, 1-2 and 1-3 Control Embodiment 1 Embodiment 4 Embodiment 7 Test Item Sample 1-0 Sample 1-1 Sample 1-2 Sample 1-3 Ultrasonic power 0 1 10 20 (W/mL) in step (2) Ultrasonic time 0 60 30 10 (min) in step (2) Extraction rate (%) 24.48b ? 2.52 43.75a ? 0.78 44.05a ? 0.75 43.21a ? 0.78 Protein content (%) 44.23a ? 1.14 43.75a ? 0.78 44.05a ? 0.75 43.21a ? 0.78 Total sugar content 36.54c ? 0.25 41.96b ? 0.85 44.12a ? 0.51 40.32b ? 0.71 Protein and total (%) 80.78c ? 1.34 85.71b ? 0.87 88.17a ? 0.74 83.53b ? 0.71 sugar content (%) Solubility (%) 78.82c ? 0.62 86.32a ? 0.38 83.38b ? 0.55 86.85a ? 0.90 Emulsifying activity 7.22a ? 0.07 6.82c ? 0.19 6.72c ? 0.04 7.07b ? 0.12 index (m.sup.2/g) Emulsifying stability 24.33c ? 0.63 34.25a ? 1.87 28.82b ? 0.25 32.07ab ? 2.86 index (min) Foaming capacity (%) 96.89c ? 2.74 122.22b ? 5.67 145.56a ? 7.93 119.33b ? 1.96 Foaming stability (%) 26.56c ? 0.60 45.48a ? 0.63 36.23b ? 5.99 49.34a ? 0.87

[0136] It can be seen in Table 1 that ultrasonic treatment in step (2) significantly improves the extraction rate, the total sugar content, the protein and total sugar content, the solubility, the emulsifying stability index, the foaming capacity and the foaming stability of sample 1-0. This is because ultrasonic wave effectively damages the cell structure of flaxseed, reduces the viscosity of the extract and thus increases the dissolution of polysaccharide.

[0137] Embodiment 4 is an optimum embodiment. The extraction rate, the emulsifying stability index and the solubility of the sample 1-2 prepared under this condition are increased by 79.94%, 18.45% and 5.79% respectively.

[0138] The test item demonstrates that ultrasonic treatment can effectively change the substance composition and functional properties of the flaxseed protein-polysaccharide natural mixture (extracted in step 1) to achieve directional extraction in different application scenarios. Based on the improvement of the emulsifying stability index and the solubility of the mixture by the test method, the powdered oil prepared by the component as an emulsifier is subsequently displayed, but the sample is not limited to the application scenario. [0139] 2. Test of powdered oil in embodiment 4

[0140] The powdered oil prepared in step (3) of embodiment 4 was photographed. The results were shown in FIG. 3.

[0141] It can be seen in FIG. 3 that the powdered oil has a white appearance and a light aroma of flaxseed, has no obvious oil leakage or caking phenomenon, and can be moistened in cold water. [0142] 3. Test of flaxseed protein-polysaccharide natural mixtures ii in embodiments 1-9

[0143] The flaxseed protein-polysaccharide natural mixtures II prepared in step (4) of embodiments 1-9 were recorded as sample 1-1-1, sample 1-1-2, sample 1-1-3, sample 1-2-1, sample 1-2-2, sample 1-2-3, sample 1-3-1, sample 1-3-2 and sample 1-3-3 respectively; and the samples without ultrasonic treatment were used as control and recorded as sample 1-1-0, sample 1-2-0 and sample 1-3-0 respectively. The extraction rate, protein content, total sugar content, protein and total sugar content, solubility, emulsifying activity index, emulsifying stability index, foaming capacity and foaming stability were tested respectively, and the results were shown in Tables 2-4.

TABLE-US-00002 TABLE 2 Test Results of Samples 1-1-0, 1-1-1, 1-1-2 and 1-1-3 Control Embodiment 1 Embodiment 2 Embodiment 3 Test Item Sample 1-1-0 Sample 1-1-1 Sample 1-1-2 Sample 1-1-3 Ultrasonic power 1 (W/mL) in step (2) Ultrasonic time 60 (min) in step (2) Ultrasonic power 0 1 10 20 (W/mL) in step (4) Ultrasonic time 0 60 30 10 (min) in step (4) Extraction rate (%) 18.34b ? 0.17 26.13a ? 0.58 24.18a ? 0.68 23.89a ? 0.71 Protein content (%) 66.73c ? 0.43 71.58a ? 0.39 69.76b ? 0.83 69.30b ? 0.92 Total sugar content (%) 12.17a ? 0.68 10.11b ? 0.38 10.66b ? 0.35 10.46b ? 0.53 Protein and total 78.90c ? 0.28 81.69a ? 0.27 80.42b ? 0.55 79.75b ? 0.80 sugar content (%) Solubility (%) 82.72b ? 0.42 93.52a ? 1.73 95.64a ? 0.57 95.11a ? 0.93 Emulsifying activity 5.67d ? 0.07 7.81a ? 0.12 6.09c ? 0.05 6.87b ? 0.14 index (m.sup.2/g) Emulsifying stability 26.84c ? 0.67 130.51a ? 15.75 112.13b ? 2.54 108.75b ? 7.58 index (min) Foaming capacity (%) 78.91d ? 1.42 94.00c ? 1.76 103.78b ? 1.68 112.00a ? 3.06 Foaming stability (%) 13.49a ? 0.71 11.83a ? 0.30 7.71b ? 0.07 7.13b ? 0.93

[0144] It can be seen in Table 2 that ultrasonic treatment in step (4) significantly improves the extraction rate, the protein content, the protein and total sugar content, the solubility, the emulsifying activity index, the emulsifying stability index and the foaming capacity of sample 1-1-0. This is because ultrasonic wave increases the dissolution of the protein.

TABLE-US-00003 TABLE 3 Test Results of Samples 1-2-0, 1-2-1, 1-2-2 and 1-2-3 Control Embodiment 4 Embodiment 5 Embodiment 6 Test Item Sample 1-2-0 Sample 1-2-1 Sample 1-2-2 Sample 1-2-3 Ultrasonic power 10 (W/mL) in step (2) Ultrasonic time 30 (min) in step (2) Ultrasonic power 0 1 10 20 (W/mL) in step (4) Ultrasonic time 0 60 30 10 (min) in step (4) Extraction rate (%) 19.69b ? 0.79 26.01a ? 1.01 28.16a ? 0.86 25.33a ? 0.65 Protein content (%) 65.84b ? 2.10 74.67a ? 0.47 73.48a ? 0.79 73.05a ? 2.52 Total sugar content 11.24c ? 0.72 12.39a ? 0.51 11.03b ? 0.34 10.83b ? 0.36 (%) Protein and total 77.08c ? 0.54 87.06a ? 0.61 84.51b ? 0.42 83.88a ? 0.31 sugar content (%) Solubility (%) 88.32b ? 0.87 94.75a ? 0.82 95.39a ? 0.48 94.32a ? 1.13 Emulsifying 5.91c ? 0.03 6.41b ? 0.01 6.56b ? 0.10 8.06a ? 0.15 activity index (m.sup.2/g) Emulsifying 45.50c ? 2.84 186.43a ? 54.80 126.33ab ? 32.88 109.81b ? 13.56 stability index (min) Foaming capacity 88.44d ? 2.04 129.78b ? 4.29 122.89c ? 1.39 153.56a ? 2.34 (%) Foaming stability 25.34c ? 0.62 24.44a ? 0.45 26.55a ? 0.95 23.82b ? 5.01 (%)

[0145] It can be seen in Table 3 that ultrasonic treatment in step (4) significantly improves the extraction rate, the protein content, the protein and total sugar content, the solubility, the emulsifying activity index, the emulsifying stability index and the foaming capacity of sample 1-2-0. This is because ultrasonic wave increases the dissolution of the protein.

TABLE-US-00004 TABLE 4 Test Results of Samples 1-3-0, 1-3-1, 1-3-2 and 1-3-3 Control Embodiment 7 Embodiment 8 Embodiment 9 Test Item Sample 1-3-0 Sample 1-3-1 Sample 1-3-2 Sample 1-3-3 Ultrasonic power 20 (W/mL) in step (2) Ultrasonic time 10 (min) in step (2) Ultrasonic power 0 1 10 20 (W/mL) in step (4) Ultrasonic time 0 60 30 10 (min) in step (4) Extraction rate (%) 20.16b ? 0.25 24.63a ? 0.87 27.94a ? 0.66 26.83a ? 0.54 Protein content (%) 67.39c ? 0.40 70.70b ? 0.50 72.03a ? 0.43 71.05b ? 0.44 Total sugar content 10.35b ? 0.55 9.34b ? 0.13 10.46b ? 0.65 12.01a ? 1.75 (%) Protein and total 77.75c ? 0.15 80.05b ? 0.55 82.49b ? 0.99 83.06a ? 0.80 sugar content (%) Solubility (%) 86.29c ? 0.88 90.63b ? 0.55 96.03a ? 0.67 94.78a ? 0.77 Emulsifying activity 6.21c ? 0.06 6.45b ? 0.05 7.08a ? 0.06 7.07a ? 0.08 index (m.sup.2/g) Emulsifying 88.17c ? 5.95 104.05a ? 7.43 91.42c ? 1.63 98.45b ? 2.76 stability index (min) Foaming capacity 58.89d ? 1.68 79.11c ? 2.69 98.44b ? 1.02 116.00a ? 1.33 (%) Foaming stability 20.03a ? 1.81 17.67a ? 2.19 10.39b ? 0.49 13.03b ? 0.22 (%)

[0146] It can be seen in Table 4 that ultrasonic treatment in step (4) significantly improves the extraction rate, the protein content, the protein and total sugar content, the solubility, the emulsifying activity index, the emulsifying stability index and the foaming capacity of sample 1-3-0. This is because ultrasonic wave increases the dissolution of the protein.

[0147] Embodiment 4 is an optimum embodiment. The extraction rate, the emulsifying activity index and the emulsifying stability index of the sample 1-2-1 prepared under this condition are increased by 32.10%, 8.46% and 309.74% respectively. In addition, the maximum total extraction rate of the flaxseed meal can reach 72.21% by two-step ultrasonic coupling, which is increased by 63.48% compared with the reference example.

[0148] The test item demonstrates that ultrasonic treatment can effectively change the substance composition and functional properties of the flaxseed protein-polysaccharide natural mixture (extracted in step 2) to achieve directional extraction in different application scenarios. Based on the improvement of the emulsifying activity index and the emulsifying stability index of the mixture by the test method, the low oil phase emulsion prepared by the component as an emulsifier is subsequently displayed, but the sample is not limited to the application scenario. [0149] 4. Test of low oil phase nanoemulsion in embodiment 4

[0150] The low oil phase nanoemulsion prepared in step (5) of embodiment 4 was photographed. The results were shown in FIG. 4.

[0151] The average particle size and PDI (polymer dispersion index) were tested respectively. The results are shown in Table 5.

TABLE-US-00005 TABLE 5 Test Results of Low Oil Phase Nanoemulsion in Embodiment 4 Emulsion Parameter Value Average particle size (nm) 203.00 ? 4.40 PDI 0.17 ? 0.03

[0152] It can be seen in FIG. 4 and Table 5 that the average particle size of the obtained low oil phase nanoemulsion can reach about 200 nm and the PDI is only 0.17, which indicates that the component has a strong capability to stabilize the nanoemulsion. The oil phase of the emulsion is only 3%, but the emulsion shows a good emulsification effect and can be used for preparing complex plant milk and other products in the future. [0153] 5. Test of flaxseed protein-polysaccharide natural mixtures I in embodiment 2

[0154] The flaxseed protein-polysaccharide natural mixtures I prepared in step (2) of embodiments 10, 13 and 16 were recorded as sample 2-1, sample 2-2 and sample 2-3 respectively; and the sample without microwave treatment was used as control and recorded as sample 2-0. The extraction rate, protein content, total sugar content, protein and total sugar content, solubility, emulsifying activity index, emulsifying stability index, foaming capacity and foaming stability were tested respectively, and the results were shown in Table 6.

TABLE-US-00006 TABLE 6 Test Results of Samples 2-0, 2-1, 2-2 and 2-3 Control Embodiment 10 Embodiment 13 Embodiment 16 Test Item Sample 2-0 Sample 2-1 Sample 2-2 Sample 2-3 Microwave power (W) 0 40 50 100 in step (2) Microwave time (min) 0 40 20 3 in step (2) Extraction rate (%) 24.90c ? 1.23 31.20a ? 0.20 31.50a ? 0.15 29.55b ? 0.12 Protein content (%) 47.11bc ? 0.71 50.33a ? 0.79 47.99b ? 0.32 46.25c ? 0.84 Total sugar content (%) 38.23d ? 0.22 39.02c ? 0.33 47.49a ? 0.72 40.18b ? 0.25 Protein and total sugar 85.34c ? 0.69 89.36b ? 1.05 94.27a ? 0.89 88.55b ? 2.05 content (%) Solubility (%) 78.38b ? 0.56 84.99a ? 2.94 85.57a ? 0.78 85.70a ? 0.23 Emulsifying activity 7.30b ? 0.11 8.34a ? 0.09 8.48a ? 0.06 8.02b ? 0.11 index (m.sup.2/g) Emulsifying stability 24.35c ? 1.76 28.39b ? 1.33 28.79b ? 0.89 35.89a ? 1.91 index (min) Foaming capacity (%) 99.44c ? 2.07 112.22b ? 4.15 135.00a ? 3.60 95.00c ? 3.60 Foaming stability (%) 25.59a ? 0.85 21.31b ? 0.93 24.68a ? 0.44 25.15a ? 0.21

[0155] It can be seen in Table 6 that low temperature microwave treatment in step (2) significantly improves the extraction rate, the total sugar content, the protein and total sugar content, the solubility, the emulsifying activity index, the emulsifying stability index and the foaming capacity of sample 2-0 (not significant for sample 2-3). Embodiment 13 is an optimum embodiment. The extraction rate and the foaming capacity of the sample 2-2 prepared under this condition are increased by 26.51% and 35.76% respectively. This is because the synergistic effect of the thermal effect and the non-thermal effect of microwaves destroys the cellular structure of flaxseed, reduces the viscosity of the extract and thus increases the dissolution of polysaccharide.

[0156] The test item demonstrates that low temperature microwave treatment can effectively change the substance composition and functional properties of the flaxseed protein-polysaccharide natural mixture (extracted in step 1) to achieve directional extraction in different application scenarios. Based on the improvement of the foaming capacity of the mixture by the test method, high-stability gel foam prepared by the component as a high efficiency foaming agent is subsequently displayed, but the sample is not limited to the application scenario. [0157] 6. Test of high-stability gel foam in embodiment 13

[0158] The high-stability gel foam prepared in step (3) of embodiment 13 was photographed. The results were shown in FIG. 5.

[0159] It can be seen in FIG. 5 that the high-stability gel foam prepared by the sample has good plasticity, and is delicate and uniform in porosity. [0160] 7. Test of flaxseed protein-polysaccharide natural mixtures II in embodiments 10-18

[0161] The flaxseed protein-polysaccharide natural mixtures II prepared in step (4) of embodiments 10-18 were recorded as sample 2-1-1, sample 2-1-2, sample 2-1-3, sample 2-2-1, sample 2-2-2, sample 2-2-3, sample 2-3-1, sample 2-3-2 and sample 2-3-3 respectively; and the samples without ultrasonic treatment were used as control and recorded as sample 2-1-0, sample 2-2-0 and sample 2-3-0 respectively. The extraction rate, protein content, total sugar content, protein and total sugar content, solubility, emulsifying activity index, emulsifying stability index, foaming capacity and foaming stability were tested respectively, and the results were shown in Tables 7-9.

TABLE-US-00007 TABLE 7 Test Results of Samples 2-1-0, 2-1-1, 2-1-2 and 2-1-3 Control Embodiment 10 Embodiment 11 Embodiment 12 Test Item Sample 2-1-0 Sample 2-1-1 Sample 2-1-2 Sample 2-1-3 Microwave power 40 (W) in step (2) Microwave time 40 (min) in step (2) Ultrasonic power 0 1 10 20 (W/mL) in step (4) Ultrasonic time 0 60 30 10 (min) in step (4) Extraction rate (%) 16.25d ? 0.72 25.12b ? 0.11 26.84a ? 0.49 22.93c ? 0.53 Protein content 63.28c ? 0.08 65.11b ? 0.01 67.81a ? 0.40 64.80b ? 0.08 (%) Total sugar content 20.32d ? 0.04 24.83a ? 0.04 22.64c ? 0.09 24.19b ? 0.15 (%) Protein and total 83.60c ? 0.14 89.93ab ? 0.04 90.45a ? 0.56 88.98b ? 0.91 sugar content (%) Solubility (%) 94.46a ? 1.73 94.90a ? 0.91 93.76a ? 1.06 94.75a ? 2.57 Emulsifying 7.60a ? 0.06 7.20b ? 0.03 6.63c ? 0.10 5.80d ? 0.05 activity index (m.sup.2/g) Emulsifying 66.39c ? 4.05 71.49c ? 5.13 251.25a ? 10.35 154.20b ? 17.17 stability index (min) Foaming capacity 58.33d ? 4.41 96.11b ? 3.47 107.22a ? 4.19 84.44c ? 1.92 (%) Foaming stability 28.40a ? 3.76 13.85b ? 0.92 2.70c ? 0.72 3.95c ? 1.60 (%)

[0162] It can be seen in Table 7 that ultrasonic treatment in step (4) significantly improves the extraction rate, the protein content, the total sugar content, the protein and total sugar content, the emulsifying stability index and the foaming capacity of sample 2-1-0. This is because ultrasonic wave increases the dissolution of the protein and the polysaccharide.

TABLE-US-00008 TABLE 8 Test Results of Samples 2-2-0, 2-2-1, 2-2-2 and 2-2-3 Control Embodiment 13 Embodiment 14 Embodiment 15 Test Item Sample 2-2-0 Sample 2-2-1 Sample 2-2-2 Sample 2-2-3 Microwave 50 power (W) in step (2) Microwave time 20 (min) in step (2) Ultrasonic power 0 1 10 20 (W/mL) in step (4) Ultrasonic time 0 60 30 10 (min) in step (4) Extraction rate 17.22b ? 0.05 26.21a ? 1.01 30.15a ? 1.89 27.96a ? 0.93 (%) Protein content 63.33c ? 0.18 64.81b ? 0.16 65.27a ? 0.13 64.68b ? 0.08 (%) Total sugar 18.18b ? 0.64 22.98a ? 0.49 24.73a ? 1.41 22.93a ? 0.30 content (%) Protein and total 81.52c ? 0.49 87.79b ? 0.64 89.99a ? 1.51 87.61b ? 0.29 sugar content (%) Solubility (%) 95.98a ? 1.98 94.78a ? 1.61 95.00a ? 1.78 94.29a ? 2.00 Emulsifying 8.06a ? 0.14 7.34b ? 0.16 6.91c ? 0.04 6.76c ? 0.11 activity index (m.sup.2/g) Emulsifying 103.41c ? 2.46 172.52b ? 25.92 270.48a ? 26.02 216.34ab ? 34.02 stability index (min) Foaming 50.56c ? 2.08 82.78b ? 2.83 116.11a ? 2.08 78.89b ? 4.16 capacity (%) Foaming stability 26.49a ? 6.00 7.32c ? 2.30 14.80b ? 2.25 2.08d ? 2.94 (%)

[0163] It can be seen in Table 8 that ultrasonic treatment in step (4) significantly improves the extraction rate, the protein content, the total sugar content, the protein and total sugar content, the emulsifying stability index and the foaming capacity of sample 2-2-0. This is because ultrasonic wave improved the dissolution of the protein and the polysaccharide.

TABLE-US-00009 TABLE 9 Test Results of Samples 2-3-0, 2-3-1, 2-3-2 and 2-3-3 Control Embodiment 16 Embodiment 17 Embodiment 18 Test Item Sample 2-3-0 Sample 2-3-1 Sample 2-3-2 Sample 2-3-3 Microwave power 100 (W) in step (2) Microwave time 3 (min) in step (2) Ultrasonic power 0 1 10 20 (W/mL) in step (4) Ultrasonic time 0 60 30 10 (min) in step (4) Extraction rate (%) 14.58c ? 1.08 24.92b ? 0.40 28.76a ? 0.37 25.19b ? 1.47 Protein content (%) 65.91c ? 0.58 66.91b ? 0.22 68.23a ? 0.18 64.86d ? 0.11 Total sugar content 16.36d ? 0.04 19.56b ? 0.26 18.99c ? 0.41 20.37a ? 0.22 (%) Protein and total 82.27c ? 0.69 86.47a ? 0.44 87.22a ? 0.18 85.24b ? 0.34 sugar content (%) Solubility (%) 95.17a ? 1.50 94.62a ? 1.17 94.89a ? 1.14 94.44a ? 2.09 Emulsifying 8.27a ? 0.08 6.82c ? 0.07 7.09b ? 0.05 6.74c ? 0.08 activity index (m.sup.2/g) Emulsifying 102.20c ? 4.37 192.14a ? 11.87 136.56b ? 17.56 129.26d ? 4.71 stability index (min) Foaming capacity 57.78c ? 3.85 113.33b ? 3.33 136.67a ? 3.33 113.56b ? 1.68 (%) Foaming stability 26.85a ? 1.60 8.32c ? 2.16 1.29d ? 1.23 11.64b ? 1.26 (%)

[0164] It can be seen in Table 9 that ultrasonic treatment in step (4) significantly improves the extraction rate, the protein content, the total sugar content, the protein and total sugar content, the emulsifying stability index and the foaming capacity of sample 3-3-0. This is because ultrasonic wave increases the dissolution of the protein and the polysaccharide.

[0165] Embodiment 14 is an optimum embodiment. The extraction rate and the emulsifying stability index of the sample 2-2-2 prepared under this condition are 75.09% and 161.56% respectively. In addition, the maximum total extraction rate of the flaxseed meal can reach 61.65% by microwave and ultrasonic coupling, which is increased by 46.37% compared with the reference example.

[0166] The test item demonstrates that ultrasonic treatment can effectively change the substance composition and functional properties of the flaxseed protein-polysaccharide natural mixture (extracted in step 2) to achieve directional extraction in different application scenarios. Based on the improvement of the emulsifying activity index and the emulsifying stability index by the test method, the foamed emulsion prepared by the component as an emulsifier is subsequently displayed, but the sample is not limited to the application scenario. [0167] 8. test of foamed emulsion in embodiment 14

[0168] The foamed emulsion prepared in step (5) of embodiment 14 was shaped and then photographed. The overflowed rate was represented by the volumes of the foamed emulsion and the emulsion before whipping. The results were shown in FIG. 6.

[0169] It can be seen in FIG. 6 that the component has good emulsifying stability index, and can stabilize coconut oil with 10% oil phase. When combined with sample 2-2, the foamed emulsion can be obtained, and the foaming rate reaches 309%. The foamed emulsion can be used for preparing plant-based ice cream or plant-based cakes in the future. [0170] 9. Test of flaxseed protein-polysaccharide natural mixtures I in embodiments 19, 22 and 25

[0171] The flaxseed protein-polysaccharide natural mixtures I prepared in step (2) of embodiments 19, 22 and 25 were recorded as sample 3-1, sample 3-2 and sample 3-3 respectively; and the sample without bio-enzyme treatment was used as control and recorded as sample 3-0. The extraction rate, protein content, total sugar content, protein and total sugar content, solubility, emulsifying activity index, emulsifying stability index, foaming capacity and foaming stability were tested respectively, and the results were shown in Table 10.

TABLE-US-00010 TABLE 10 Test Results of Samples 3-0, 3-1, 3-2 and 3-3 Control Embodiment 19 Embodiment 22 Embodiment 25 Test Item Sample 3-0 Sample 3-1 Sample 3-2 Sample 3-3 Bio-enzyme type None Pectase Cellulase Hemicellulase in step (2) Extraction rate 26.87d ? 1.89 46.87a ? 0.98 31.30c ? 0.29 38.84b ? 0.68 (%) Protein content 48.39a ? 1.17 36.81d ? 1.06 44.27b ? 0.36 40.67c ? 0.48 (%) Total sugar 35.77d ? 0.21 42.98b ? 0.64 39.54c ? 0.13 45.59a ? 0.72 content (%) Protein and total 84.17ab ? 1.14 79.80c ? 1.57 83.81ab ? 0.35 86.26a ? 1.20 sugar content (%) Solubility (%) 79.41c ? 1.40 94.92a ? 0.57 92.85b ? 0.64 91.94b ? 1.31 Emulsifying 7.20d ? 0.11 7.93b ? 0.25 7.97b ? 0.16 8.23a ? 0.17 activity index (m.sup.2/g) Emulsifying 25.88c ? 0.31 84.73c ? 0.08 87.20b ? 9.84 178.89a ? 19.22 stability index (min) Foaming capacity 101.56a ? 1.37 93.78b ? 2.79 95.11b ? 1.37 37.78c ? 0.83 (%) Foaming stability 26.04a ? 0.48 16.76c ? 0.68 26.21a ? 0.36 23.49b ? 1.84 (%)

[0172] It can be seen in Table 10 that different bio-enzyme treatment significantly improves the extraction rate, the total sugar content, the solubility, the emulsifying activity index and the emulsifying stability index of sample 3-0. This is because enzymolysis damages the cell structure of flaxseed or conducts enzymatic hydrolysis for macromolecules to form micromolecules, thus reduces the viscosity of the extract and increases the dissolution of polysaccharide.

[0173] Embodiment 19 is an optimum embodiment. The extraction rate and the solubility of the sample 3-1 prepared under this condition are increased by 74.43% and 19.53% respectively.

[0174] The test item demonstrates that the addition of the bio-enzyme can effectively change the substance composition and functional properties of the flaxseed protein-polysaccharide natural mixture (extracted in step 1) to achieve directional extraction in different application scenarios. Based on the improvement of the solubility of the mixture by the test method, the low-turbidity solution of the component is subsequently displayed, and can be added to low-turbidity plant protein drinks in the future, but the sample is not limited to the application scenario. [0175] 10. Test of low-turbidity plant protein drinks in embodiments 19, 22 and 25

[0176] The use amounts of the flaxseed protein-polysaccharide natural mixtures I prepared in step (3) of embodiments 19, 22 and 25 were changed, and solutions with mass percent of 0.1%, 0.5%, 1%, 1.5% and 2% were prepared respectively and photographed. The results were shown in FIG. 7.

[0177] It can be seen in FIG. 7 that bio-enzyme treatment greatly reduces the turbidity of the product solution, wherein pectase has the best effect. Samples treated with different bio-enzymes can be added as a nutritional supplement to the low-turbidity plant protein drinks. In addition, because oligosaccharide is produced by enzymolysis, this additive component may also have certain biological activity. [0178] 11. Test of flaxseed protein-polysaccharide natural Mixtures II in embodiments 19-27

[0179] The flaxseed protein-polysaccharide natural mixtures II prepared in step (4) of embodiments 19-27 were recorded as sample 3-1-1, sample 3-1-2, sample 3-1-3, sample 3-2-1, sample 3-2-2, sample 3-2-3, sample 3-3-1, sample 3-3-2 and sample 3-3-3 respectively; and the samples without ultrasonic treatment were used as control and recorded as sample 3-1-0, sample 3-2-0 and sample 3-3-0 respectively. The extraction rate, protein content, total sugar content, protein and total sugar content, solubility, emulsifying activity index, emulsifying stability index, foaming capacity and foaming stability are tested respectively, and the results were shown in Tables 11-13.

TABLE-US-00011 TABLE 11 Test Results of Samples 3-1-0, 3-1-1, 3-1-2 and 3-1-3 Control Embodiment 19 Embodiment 20 Embodiment 21 Test Item Sample 3-1-0 Sample 3-1-1 Sample 3-1-2 Sample 3-1-3 Bio-enzyme type in Pectase step (2) Ultrasonic power 0 1 10 20 (W/mL) in step (4) Ultrasonic time (min) 0 60 30 10 in step (4) Extraction rate (%) 17.98c ? 0.18 18.81b ? 0.09 18.87b ? 0.21 19.61a ? 0.30 Protein content (%) 50.36c ? 0.35 52.53b ? 0.34 54.28a ? 0.08 50.11c ? 0.39 Total sugar content 18.65d ? 0.09 24.36a ? 0.38 20.18c ? 0.15 20.94b ? 0.28 (%) Protein and total sugar 69.01d ? 0.44 76.89a ? 0.71 74.46b ? 0.21 71.05c ? 0.63 content (%) Solubility (%) 93.90b ? 0.61 95.01a ? 0.98 95.30a ? 1.45 93.96b ? 0.36 Emulsifying activity 7.49c ? 0.05 10.38a ? 0.22 7.66b ? 0.04 7.65b ? 0.04 index (m.sup.2/g) Emulsifying stability 78.53a ? 8.36 19.68b ? 1.98 20.50b ? 3.32 22.83b ? 1.32 index (min) Foaming capacity (%) 61.11a ? 5.57 35.56b ? 4.63 38.00b ? 4.46 58.89a ? 5.57 Foaming stability (%) 25.06c ? 4.59 45.87b ? 5.20 41.11b ? 3.19 65.67a ? 3.32

[0180] It can be seen in Table 11 that ultrasonic treatment in step (4) significantly improves the extraction rate, the protein content (not significant for sample 3-1-3), the total sugar content, the protein and total sugar content, the solubility, the emulsifying activity index and the foaming stability of sample 3-1-0. This is because ultrasonic wave increases the dissolution of the polysaccharide.

TABLE-US-00012 TABLE 12 Test Results of Samples 3-2-0, 3-2-1, 3-2-2 and 3-2-3 Control Embodiment 22 Embodiment 23 Embodiment 24 Test Item Sample 3-2-0 Sample 3-2-1 Sample 3-2-2 Sample 3-2-3 Bio-enzyme Cellulase type in step (2) Ultrasonic 0 1 10 20 power (W/mL) in step (4) Ultrasonic time 0 60 30 10 (min) in step (4) Extraction rate 19.31c ? 0.19 22.47b ? 0.73 23.41a ? 0.42 19.71c ? 0.21 (%) Protein content 56.48b ? 0.15 58.17a ? 0.39 53.32c ? 0.44 49.44d ? 0.83 (%) Total sugar 22.24c ? 0.42 26.52a ? 0.07 25.07b ? 0.15 26.38a ? 0.13 content (%) Protein and total 78.72b ? 0.33 84.69a ? 0.32 78.39b ? 0.55 75.82c ? 0.90 sugar content (%) Solubility (%) 96.46c ? 1.06 97.83b ? 0.83 98.49a ? 0.58 97.40b ? 0.62 Emulsifying 5.91c ? 0.08 6.34b ? 0.11 6.52b ? 0.07 6.93a ? 0.07 activity index (m.sup.2/g) Emulsifying 86.94c ? 4.73 147.53a ? 19.79 128.01b ? 2.06 140.83a ? 10.07 stability index (min) Foaming 53.78b ? 7.39 49.56b ? 3.00 63.56a ? 6.36 58.67ab ? 3.81 capacity (%) Foaming 27.36a ? 4.26 15.49b ? 1.84 16.93b ? 0.62 18.33b ? 2.35 stability (%)

[0181] It can be seen in Table 12 that ultrasonic treatment in step (4) significantly improves the extraction rate (not significant for sample 3-2-3), the total sugar content, the protein and total sugar content (not significant for sample 3-2-2 and sample 3-2-3), the solubility, the emulsifying activity index and the emulsifying stability index of sample 3-2-0. This is because ultrasonic wave increases the dissolution of the polysaccharide.

TABLE-US-00013 TABLE 13 Test Results of Samples 3-3-0, 3-3-1, 3-3-2 and 3-3-3 Control Embodiment 25 Embodiment 26 Embodiment 27 Test Item Sample 3-3-0 Sample 3-3-1 Sample 3-3-2 Sample 3-3-3 Bio-enzyme type in Hemicellulase step (2) Ultrasonic power 0 1 10 20 (W/mL) in step (4) Ultrasonic time (min) 0 60 30 10 in step (4) Extraction rate (%) 16.69c ? 0.30 19.21a ? 0.31 18.22b ? 0.81 18.86b ? 0.30 Protein content (%) 47.23a ? 0.64 48.68a ? 0.70 44.04c ? 0.99 46.43b ? 0.38 Total sugar content 21.21d ? 0.39 32.38a ? 0.43 26.18b ? 0.18 25.10c ? 0.30 (%) Protein and total 68.44c ? 1.03 81.06a ? 0.87 70.22b ? 0.99 71.52b ? 0.54 sugar content (%) Solubility (%) 94.04a ? 0.56 93.63a ? 0.12 93.38a ? 0.25 94.83a ? 1.04 Emulsifying activity 8.38c ? 0.06 8.40c ? 0.29 10.21b ? 0.15 9.79a ? 0.06 index (m.sup.2/g) Emulsifying stability 33.39a ? 3.01 30.54a ? 2.67 15.99b ? 0.24 24.87b ? 0.35 index (min) Foaming capacity (%) 111.11a ? 4.16 81.11b ? 5.67 86.22b ? 5.48 85.11b ? 4.76 Foaming stability (%) 35.27b ? 9.36 58.55a ? 3.41 42.30b ? 3.68 40.75b ? 3.03

[0182] It can be seen in Table 13 that ultrasonic treatment in step (4) significantly improves the extraction rate, the total sugar content, the protein and total sugar content, the solubility, the emulsifying activity index (not significant for sample 3-3-1) and the foaming stability of sample 3-3-0. This is because ultrasonic wave increases the dissolution of the polysaccharide.

[0183] Embodiment 22 is an optimum embodiment. The extraction rate, the solubility and the total amount of the protein and the polysaccharide of the sample 3-2-1 prepared under this condition are increased by 16.36%, 1.42% and 0.76% respectively. In addition, the maximum total extraction rate of the flaxseed meal can reach 70.28% by bio-enzyme-ultrasonic coupling, which is increased by 52.19% compared with the reference example.

[0184] The test item demonstrates that ultrasonic treatment can effectively change the substance composition and functional properties of the flaxseed protein-polysaccharide natural mixture (extracted in step 2) to achieve directional extraction in different application scenarios. Based on the improvement of the solubility and the nutritional ingredient of the mixture by the test method, the component as a nutritional supplement is added to the meal replacement milkshake, but the sample is not limited to the application scenario. [0185] 12. test of meal replacement milkshake in embodiment 22

[0186] The meal replacement milkshake prepared in step (5) of embodiment 22 was photographed. The results were shown in FIG. 8.

[0187] It can be seen in FIG. 8 that the meal replacement milkshake has no obvious caking, and has good flowability.

[0188] Physical and chemical properties were tested, and the results were shown in Table 14.

TABLE-US-00014 TABLE 14 Test Results of Meal Replacement Milkshake in Embodiment 22 Physical and Chemical Properties Value Dispersibility/s 84.58 ? 1.27 Wettability/s 76.7 ? 7.39

[0189] It can be seen in Table 14 that the wettability and the dispersibility of the meal replacement milkshake are good. [0190] 13. test of flaxseed protein-polysaccharide natural mixtures I and II in reference example

[0191] The flaxseed protein-polysaccharide natural mixture I prepared in step (2) and the flaxseed protein-polysaccharide natural mixture II prepared in step (3) in the reference example were taken, and the extraction rate, protein content, total sugar content, protein and total sugar content, solubility, emulsifying activity index, emulsifying stability index, foaming capacity and foaming stability were tested respectively. The results were shown in Table 15.

TABLE-US-00015 TABLE 15 Test Results of Flaxseed Protein-Polysaccharide Natural mixtures I and II in Reference Example Flaxseed Flaxseed Protein-Polysaccharide Protein-Polysaccharide Test Item Natural mixture I Natural mixture II Extraction rate (%) 24.48 ? 0.52 20.10 ? 0.16 Protein content (%) 44.23 ? 1.14 59.84 ? 0.44 Total sugar content (%) 36.54 ? 0.25 21.24 ? 0.72 Protein and total sugar 80.78 ? 1.34 81.08 ? 0.28 content (%) Solubility (%) 78.82 ? 0.62 77.27 ? 1.21 Emulsifying activity 7.22 ? 0.07 7.97 ? 0.05 index (m.sup.2/g) Emulsifying stability 24.33 ? 0.63 20.21 ? 0.26 index (min) Foaming capacity (%) 96.89 ? 2.74 52.33 ? 2.33 Foaming stability (%) 26.56 ? 0.60 47.72 ? 1.06

[0192] It can be seen in Table 15 that the total extraction rate of the flaxseed meal is only 44.58% by two-step extraction in the reference example.

[0193] The above contrast tests indicate that compared with the traditional extraction mode of the flaxseed protein, the method of the present invention can extract active components from the flaxseed meal more efficiently, greenly and moderately, solves the problem of low extraction rate of the flaxseed protein-polysaccharide natural mixture from water extraction, can obtain flaxseed protein-polysaccharide natural mixtures of different proportions and properties, enriches the orientability of the extraction process and can realize the multi-scenario utilization of different components.

[0194] The above description of the disclosed embodiments enables those skilled in the art to realize or use the present invention. Many modifications to these embodiments will be apparent to those skilled in the art. The general principle defined herein can be realized in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principle and novel features disclosed herein.