Spreading device, method and powder-like mixture composition for controlling or preventing forest pathogens on tree stumps

Abstract

A spreading device, a powder-like mixture composition and a method for spreading the composition for control or prevent forest pathogens on tree stumps are disclosed.

Claims

1. A spreading device (1) adapted to administer onto a tree stump (T) a composition which comprises microbial propagules to control or prevent the growth of a forest pathogen, which spreading device comprises: a spreading end (3) connected to a pipe-like frame (2), and administering means (4) for administering said composition, wherein said administering means (4) comprise a pressure accumulator (42) for loading pressurized air (I), said pressure accumulator (42) being connected to a first end of the pipe-like frame (2) via a supply pipe (413) and regulating means (41) which comprises at least one regulating valve (411) for regulating flow of the pressurized air (I) entering the frame (2) which regulating means (41) further comprises communication means (47) for providing to the at least one regulating valve (411) data about starting time of pressurized air (I) delivery and data about duration of said pressurized air (I) delivery, wherein said pressure of pressurized air depends on the diameter of the tree stump and which administering means (4) further comprises: a powder storage (45) which contains a powder-like mixture (S, K) comprising microbial propagule (S) and a solid fine carrier (K), which powder storage (45) is configured to be capable of being brought into a flow connection of the powder-like mixture with the frame (2), and a control apparatus (43) connected to a second end of the frame (2) for administering the powder-like mixture (S, K) inside the frame (2) and means for blowing the powder-like mixture (S, K) onto a tree stump, via a spreading head (3) connected to the frame (2).

2. The spreading device according to claim 1, wherein the communication means (47) further enables providing a control apparatus (43) with the data relating to starting time and duration of the dosage of powder-like mixture (S, K) inside the frame (2).

3. The spreading device according to claim 1, wherein the regulating means (41) comprises two regulating valves; a first regulating valve (411a) before the pressure accumulator (42) and a second regulating valve (411b) after the pressure accumulator in the flow direction of pressurized air (I).

4. The spreading device according to claim 3, wherein at least one regulating valve (411) is electromagnetic valve, preferably the first and the second regulating valves (411) are electromagnetic valves, wherein the first electromagnetic valve (411a) which locates before the pressure accumulator (42) defines pressure level of pressurized air (I) in the pressure accumulator (42) and the second electromagnetic valve (411b) which locates after the pressure accumulator (42) defines the starting time of delivery of pressurized air into frame (2) of the spreading device.

5. The spreading device according to claim 1, wherein said communication means (47) operate automatically and receive data from a harvester control system, which data comprises control commands for starting time of delivery and the pressure of pressurized air (I) and/or starting time and duration of dosage of the powder-like mixture (S, K).

6. The spreading device according to claim 5, wherein the control commands received from the harvester control system (100) comprises control commands relating to working hydraulics (105) of a harvester head which controls a hydraulic circuit (91, 97) connected to usage of a chain saw or/and usage of tree stump treatment device for liquid.

7. The spreading device according to claim 6, wherein control commands are arranged to be generated by or derived from valve(s) which open/close fluid pressure connection between a hydraulic pump and hydraulic actuators of a chain saw (5) or control commands are arranged to be generated by or derived from a pressure gauge in a pipe/tube of hydraulic circuit (91) of the chain saw (5).

8. The spreading device according to claim 5, wherein control commands from harvester control system (100) which relate to pressure level of delivered pressurized air and/or dosage of the powder-like mixture (S, K) depend on the trunk shape/diameter specifically depend on diameter of cut tree stump (T) diameter.

9. The spreading device according claim 5, wherein the pressure of the pressurized air (I) to be loaded into the pressure accumulator (42) depends on the dimension of the tree stump T and optionally also the duration of the delivery of pressurized air (I) and can be commenced automatically by action of the communication means.

10. The spreading device (1) according to claim 1, wherein said powder storage (45) further comprises a container (451) configured to receive a powder cartridge (452) comprising a powder-like mixture of microbial propagules, a culture medium (S) used for producing the microbial propagules and a fine solid carrier (K), preferably said microbial propagules are fungal spores, and preferably said solid fine carrier (K) has a volumetric weight of at least 0.50 g/cm.sup.3, or at least 0.60 g/cm.sup.3 or at least 1 g/cm.sup.3.

11. The spreading device (1) according to claim 10, wherein said powder cartridge (452) further contains a removable cap and said powder storage contains means (453) for bringing said powder-like mixture (S, K) in flow connection with the inside of the frame (2) after removing said cap.

12. The spreading device according to claim 11, wherein said powder cartridge (452) is a tube made of plastic, cardboard or thin metal.

13. The spreading device (1) according to claim 11, wherein said container (451) is connected to flow connection of the powder-like mixture with inside of the frame (2) via a supply tube (453).

14. The spreading device (1) according to claim 10, wherein said fungal spores are spores of Phlebiopsis gigantea; preferably spores Phlebiopsis gigantea strain ATCC 90304, Phlebiopsis gigantea strain DSMZ 26191, Phlebiopsis gigantea strain DSMZ 26192 or Phlebiopsis gigantea strain DSMZ 16201, or a combination thereof.

15. The spreading device (1) according to claim 1, wherein said regulating valve (41) of the administering means (4) comprises a magnetic valve (411a) and/or a damper for altering the volumetric flow and/or pressure of pressurized air (I) entering the frame (2), and the magnetic valve is preferably an on/off-valve.

16. The spreading device (1) according to claim 1, wherein said control apparatus (43) comprises a stepper motor (431) or a servo motor.

17. A powder cartridge (452) containing a powder-like mixture, which powder cartridge (452) is adapted to be used in the spreading device (1) of claim 1, wherein said powder-like mixture of the powder cartridge can be brought into the flow connection with the frame (2) of the spreading device (1) by the usage of a control apparatus (43), which powder-like mixture comprises microbial propagules, preferably fungal spores, and a solid fine carrier, wherein said solid fine carrier has volumetric weight of at least 0.50 g/cm.sup.3, or at least 0.60 g/g/cm.sup.3, or at least 1 g/cm.sup.3 and wherein the amount of said powder-like mixture to be administered on the tree stump is arranged to be depend on diameter of the tree stump (T) to be treated.

18. The powder cartridge (452) according to claim 17, wherein said powder cartridge comprises 5 to-50% w-% of fungal spores and 5 to-90 w-% of a solid fine carrier, preferably 10 to 30% w-% of fungal spores and 20 to 80% w-% of a solid fine carrier; and optionally 0.5 to 5% of a marker dye or 0.5 to 20% of a nutritional supplement, or a mixture thereof, preferably comprising lignin and lignin derivatives, such as lignosulphonate or lignohumate.

19. The powder cartridge (452) according to claim 17, wherein said fungal spores are in combination with a culture medium (S) used for producing the fungal spores, and the culture medium optionally comprises amorphous silica.

20. The powder cartridge (452) according to claim 17, wherein said fungal spores are spores of Phlebiopsis gigantea; preferably spores of Phlebiopsis gigantea strain ATCC 90304, Phlebiopsis gigantea strain DSMZ 26191, Phlebiopsis gigantea strain DSMZ 26192 or Phlebiopsis gigantea strain DSMZ 16201, or a combination thereof.

21. The powder cartridge (452) according to claim 17, wherein said solid fine carrier (S) is lime, calcium carbonate, kaolin, bentonite, talc, gypsum, chitosan, vermiculite, perlite, amorphous silica, granular clay, fine-grained cellulose powder, polysaccharides, chitin, chitosan, polyacrylamide, lignin derivatives, cereal flours, yeast extract, betaine or fine-grained composition made of cereal grains, bran, sawdust, peat or wood chips or a mixture thereof; preferably calcium carbonate.

22. A composition in the form of a powder-like mixture (S, K), wherein said composition comprises microbial propagules and a solid fine carrier wherein said microbial propagules are fungal spores which control or prevent the growth of forest pathogens on a tree stump and wherein said solid fine carrier has a volumetric weight of at least 0.50 g/cm.sup.3, or at least 0.60 g/cm.sup.3, or at least 1 g/cm.sup.3.

23. The composition according to claim 22, wherein said composition comprises 5 to 50% w-% of fungal spores and 5 to 90 w-% of a solid fine carrier, preferably 10 to 30% w-% of fungal spores and 20 to 80% w-% of a solid fine carrier, and optionally 0.5 to 5% of a marker dye or 0.5 to 20% of a nutritional supplement, or a mixture thereof, preferably comprising lignin and lignin derivatives, such as lignosulphonate or lignohumate.

24. The composition of claim 22, wherein said fungal spores are in combination with a culture medium (S) used for producing the fungal spores, and the culture medium optionally comprising amorphous silica.

25. The composition according to claim 22, wherein said fungal spores are spores of Phlebiopsis gigantea; preferably spores of Phlebiopsis gigantea strain ATCC 90304, Phlebiopsis gigantea strain DSMZ 26191, Phlebiopsis gigantea strain DSMZ 26192 or Phlebiopsis gigantea strain DSMZ 16201, or a combination thereof.

26. The composition according to claim 22, wherein said solid fine carrier is lime, calcium carbonate, kaoline, bentonite, talc, gypsum, chitosan, vermiculite, perlite, amorphous silica, granular clay, fine-grained cellulose powder, polysaccharides, chitin, chitosan, polyacrylamide, lignin derivatives, cereal flours, yeast extract, betaine or fine-grained composition made of cereal grains, bran, sawdust, peat or wood chips or a mixture thereof, preferably calcium carbonate.

27. A method to control or prevent or control growth of Heterobasidon fungal pathogen on a tree stump, the method comprising the steps of: a) providing a composition according to claim 22; and b) spreading or dispersing of the composition in a powder-like mixture on said tree stump by machine or manually, wherein the spreading in step b) is at least 1 mg/cm.sup.2, or at least 2 mg/cm.sup.2, or at least 3 mg/cm.sup.2 of the powder-like mixture is spread onto the tree stumps of logging area.

28. The method of claim 27, wherein the composition in step a) comprises 5 to-50% w-% of fungal spores and 5-90 w-% of a solid fine carrier, preferably 10 to 30% w-% of fungal spores and 20 to 80% w-% of a solid fine carrier, and optionally 0.5 to 5% of a marker dye or 0.5 to 20% of a nutritional supplement, or a mixture thereof, preferably comprising lignin and lignin derivatives, such as lignosulphonate or lignohumate.

29. The method of claim 28, wherein aid fungal spores are spores of Phlebiopsis gigantea; preferably spores of Phlebiopsis gigantea strain ATCC 90304, Phlebiopsis gigantea strain DSMZ 26191, Phlebiopsis gigantea strain DSMZ 26192 or Phlebiopsis gigantea strain DSMZ 16201, or a combination thereof.

30. A method for spreading or dispersing onto a tree stump a powder-like mixture comprising microbial propagules which control or prevent the growth of a forest pathogen and a solid fine carrier having a volumetric weight of at least 0.50 g/cm.sup.3, or at least 0.60 g/cm.sup.3 or at least 1 g/cm.sup.3, wherein said method comprises the following steps: receiving data about dosage of a powder-like mixture (S, K) by regulating means (41), dosing the powder-like mixture (S, K) to an amount which depends on the diameter of the tree stump, forming an aerosol (A) from pressurized gas and the powder-like mixture by delivering pressurized gas on adjusted rate and amount into the powder like-mixture (S, K), reading or dispersing the aerosol (A) onto a tree stump by means of pressurized gas flow, wherein said pressurized gas is preferably pressurized air (I).

31. The method according to claim 30 for spreading or dispersing onto a tree stump a powder-like mixture comprising microbial propagules and a solid fine carrier, having a volumetric weight of at least 0.50 g/cm.sup.3, or at least 0.60 g/cm.sup.3 or at least 1 g/cm.sup.3, with the spreading device (1) of in claim 1, comprising in the powder storage (45) a powder cartridge (452), which contains a powder-like mixture, which comprises microbial propagules and a solid fine carrier, wherein said method comprises following steps: receiving data about amount or/and administering time and duration of the powder-line mixture (S, K) by the regulating means (41) which amount and/or administering time depends on the diameter of the tree stump, dosaging of powder-like mixture inside the frame (2) by means of the control apparatus (43), defining automatically pressure level of air to be loaded into pressure accumulator (42), loading pressurized air into pressure accumulator (42) when dosaging powder-like mixture inside the frame (2), forming an aerosol (A) from pressurized air (I) and the powder-like mixture, by delivering pressurized air (I) with and preferably automatically adjusted pressure level of a duration into the powder-like mixture present inside the frame (2), and spreading or dispersing the aerosol (A) to the tree stump by means of the pressurized air (I) flow.

32. The method according to claim 31, wherein said the method further comprises arranging pressurized air (I) to flow automatically inside the frame (2) and onward to the spreading end (3) from pressure accumulator (42) by means of at least one, preferably by means of two regulating valves (411).

33. The method according to 31, wherein said method comprises receiving control commands relating to starting time and pressure level of delivered pressurized air and/or dosage of the powder-like mixture from the harvester control system preferably from the control commands of harvester control system comprises control commands relating to working hydraulics of the harvester head which controls the usage of the chain saw or/and usage of tree stump treatment device for liquid.

34. The method according to claim 31, wherein control commands of the usage of the chain saw or/and usage of tree stump treatment device for liquid are based on the hydraulic circuit which are connected to actuators controlling the actions of chain saw (5) of the harvester head (6).

35. The method according to claim 34, wherein control command defining the beginning of the loading of accumulator (42) and dosage of powder-like mixture (S, K) inside the frame (2) s derived or generated from the hydraulic circuit (91) which controls the actuators which employs the actions of chain saw (5) when the bar of the chain saw (5) reaches the starting point (P2) at its path (P) in cutting a tree stump (T) from a standing tree.

36. The method according to claim 34, wherein control commands defining the end of the loading of accumulator (42) and dosage of the powder-like mixture (S, K) inside frame is also derived or generated from the hydraulic circuit (91) which controls the actuators employing the chain saw (5), after the bar (5) of the chain saw (5) reaches the end point at its path (P) in cutting a tree stump (T) from a standing tree.

37. The method according to claim 34, wherein control command defining the delivery of the pressurized air (I) into the frame (2) and among the powder-like mixture (S,K) which has been dosaged therein before, is generated or derived from the hydraulic circuit (91) which controls the actuators employing the chain saw (5) after the chain saw (5) bar (51) reaches the starting (P2) at its path (P) in sawing a tree stump (T) from a standing tree.

38. The method according to claim 34, wherein said control commands from harvester control system (100) relate to pressure level of delivered pressurized air and/or dosage of the powder-like mixture (S, K) which control commands further depend on the trunk shape/diameter.

39. The method of claim 38, wherein the composition comprises 5 to-50% w-% of fungal spores and 5-90 w-% of a solid fine carrier, preferably 10 to 30% w-% of fungal spores and 20 to 80% w-% of a solid fine carrier, and optionally 0.5 to 5% of a marker dye or 0.5 to 20% of a nutritional supplement, or a mixture thereof.

40. The method of claim 38, wherein said fungal spores in said powder cartridge are in combination with a culture medium (S) used for producing the spores and said culture medium optionally comprises amorphous silica.

41. The method of claim 40, wherein said fungal spores are spores of Phlebiopsis gigantean, preferably spores of Phlebiopsis gigantea strain ATCC 90304, Phlebiopsis gigantea strain DSMZ 26191, Phlebiopsis gigantea strain DSMZ 26192 or Phlebiopsis gigantea strain DSMZ 16201, or a combination thereof.

42. The method according claim 41, wherein said solid fine carrier is lime, calcium carbonate, kaoline, bentonite, talc, gypsum, chitosan, vermiculite, perlite, amorphous silica, granular clay, fine-grained cellulose powder, polysaccharides, chitin, chitosan, polyacrylamide, lignin derivatives, cereal flours, yeast extract, betaine or fine-grained composition made of cereal grains, bran, sawdust, peat or wood chips or a mixture thereof, preferably calcium carbonate.

43. The method according to claim 39, wherein said nutritional supplement comprises lignin and lignin derivatives, such as lignosulphonate or lignohumate.

Description

SHORT DESCRIPTION OF THE FIGURES

[0046] FIG. 1A shows an applicator according to the invention in a perspective view.

[0047] FIG. 1B shows an applicator according to FIG. 1, viewed from the direction IB of FIG. 1A.

[0048] FIG. 1C shows an applicator according to FIG. 1, viewed from the direction IC of FIG. 1A.

[0049] FIG. 2 shows the applicator presented in FIGS. 1A-1C as an exploded view.

[0050] FIG. 3 shows schematically the principle of forming aerosol from dry matter.

[0051] FIG. 4 shows a sieve analysis of lime.

[0052] FIG. 5 illustrates schematically the control system and measurements of the harvester head.

[0053] FIGS. 6A and 6B show the general method of the present invention.

[0054] FIG. 7 illustrates schematically spreading device and a chain saw, as viewed from a side and at a beginning of the present method.

DETAILED DESCRIPTION OF THE INVENTION

[0055] In the following, the main structures of an applicator according to the invention are first described with reference to FIGS. 1-3.

[0056] FIGS. 1A-1C show fungal spore applicator (spreading device) 1 according to the invention from slightly different angles of view but without any regulating means 41 or pressure accumulator needed for controlling and storing of the pressurized air (I). The feeding of pressurized air I is illustrated in FIG. 3. FIG. 2 shows the spreading device as an exploded view.

[0057] Spreading device 1, such as aerosol applicator, has a tubular frame 2. Aerosol spreading head 3, is associated with the first end of tubular frame 2 seen in the forefront. Frame 2 also includes administering means 4 for forming a spore-containing aerosol A which is applied using spreading head 3, equipped with a back-pressure valve, onto a tree stump T. Tubular frame 2 may also include measurement means 21 such as measuring devices for measuring flow, pressure and temperature of pressurized air. Through the measurement data obtained from these measuring devices, the formation of aerosol A from the pressurized air I and the powder-like mixture S, K to be combined with it can be adjusted first. Subsequently, information received from the measuring means 21 can still be used while applying the formed aerosol A onto a tree stump in a manner described later.

[0058] Administering means 4 for dispensing of pressurized air I and powder-like mixture S, K comprises regulating means 4; 41 associated with the frame 2 of the aerosol applicator 1; for adjusting the volume flow of pressurized air I entering frame 2 via a pressurized air supply pipe 413

[0059] The volume flow of pressurized air I may be adjusted using valve 4; 415 such as solenoid valve shown in FIGS. 1A and 1B. However, in a preferred embodiment of the present invention, the volume flow of the pressurized air I is controlled automatically using a pressure accumulator 42 and electromagnetic on/off valves 411; 411a and 411; 411b shown in FIG. 3. Administering means 4 also includes pressure accumulator 42 of pressurized air I for generating and supplying pressurized air I to electromagnetic valve 411; 411a, 411b as well as further inside of the frame 2, through a short supply pipe 413 of pressurized air (compare to FIG. 3).

[0060] Administering means 4 further comprises communication means 47 (FIG. 3) for providing to the regulating means 41, preferable electromagnetic regulating valves, for example, data about starting time of pressurized air I delivery and data about pressure level of said pressurized air I. Said communication means 47 also provide the control apparatus 43 with the data relating to starting time and duration of the dosage of powder-like mixture S, K inside the frame 2. The pressure of delivered pressurized air and dosage of powder-like mixture S, K depends primarily on the diameter of the tree stump T to be sawed from a trunk (see FIG. 7). Communication means are preferably wireless communication means which comprise a transmitter and a receiver of an electromagnetic signal. Suitable wireless communication means comprises of a receiver in connection of electromagnetic valve or control apparatus and an electromagnetic signal transmitter in connection with chain saw hydraulic circuit 91 or in connection with a hydraulic circuit controlling the actuator affecting to a stump treatment device for liquid 97 (see FIG. 5).

[0061] Administering means 4 further comprises control apparatus 43, incorporating a stepper motor 431; 431a to dispense/dosage powder-like mixture S, K onto the inside of frame 2. The aerosol A can thereafter be formed on the inside of frame 2 and dispensed to the target, i.e. to the tree stump, by supplying a burst of pressurized air I flow inside of frame via supply pipe 413.

[0062] Tubular frame 2 also includes a powder storage 4; 45 communicating with the inside of body 2 by means of supply tube 453 shown in exploded view of FIG. 2. Powder storage 45 comprises container 451 having powder cartridge 452, which contains powdery mixture being powder-like mixture S, K. The powder-like mixture S, K is composed of a mixture of Phiebiopsis gigantea spores, in the presence or not of the culture medium S used for producing the spores, mixed with a heavier solid powdery carrier K (compare FIG. 3). Powder storage 45 is operatively coupled with the previously described control apparatus 43 having a stepper motor 431 for dispensing and bringing the powder-like mixture S, K to the inside of frame 2 with supply tube 453. The powder-like mixture S, K arrives from container 451 to the inside of tubular frame 2 along supply tube 453.

[0063] Pressurized air I is then supplied from pressure accumulator 42 via pressurized air supply pipe 413 first to the inside of frame 2 and from there to spreading head 3.

[0064] Regulating means 41 of pressurized air I may also be used for adjusting the rate of powder-like mixture S, K coming from the powder storage 45 in a case regulating means comprises solenoid valve or like. However, normally regulating means 41 began their action only after powder-like mixture S, K have been dispended inside of the frame by means of the stepper motor 431.

[0065] The regulating means 41 are controlled by communication means 47, which are used delivering proper control commands to regulating valve(s) 411 for dispensing of pressurized air (compressed air) I and to control apparatus 43 for adjusting the dosage of powder-like mixture by the use powder cartridge 452. This is now illustrated more accurately in referring to FIGS. 3, 5, 6A-6B and 7. Powder-like mixture S, K contains fine carrier K and microbial propagules S, which, in turn, contains about 5% of water and is formed by the possible culture medium and the fungal spores.

[0066] The more general method of the invention is first presented by referring to FIGS. 5, 6A-6B. Said method comprises first receiving data about dosage of the powder-like mixture (S, K) by the regulating means 41.

[0067] In a general method according to present invention in a tree stump T is spread or dispersed a powder-like mixture, which comprises microbial propagules which control or prevent the growth of a forest pathogen and a solid fine carrier having a volumetric weight of at least at least 0.50 g/cm.sup.3, preferable at least 0.60 g/cm.sup.3 or most preferable at least 1 g/cm.sup.3 Said method comprises the following steps: [0068] receiving data about dosage of a powder-like mixture S, K by the regulating means 41, [0069] dosing the powder-like mixture S, K inside the frame 2 of an amount which depends on the diameter of the tree stump, [0070] forming an aerosol A from pressurized air I and a powder-like mixture S, K by delivering pressurized air having an adjusted level into the powder like-mixture S, K, [0071] spreading or dispersing the formed aerosol (A) onto a tree stump by means of pressurized air (I) flow.

[0072] As can be from the above general method, the amount of powder-like mixture S, K dispensed automatically inside the frame 2 depends on the diameter of the tree stump T. Also, the pressure level and starting time of pressurized air I delivered automatically inside the frame 2 depends on the diameter of the tree stump T. Proper control commands to regulating means 41, which control the delivery of pressurized air I inside the frame 2 is done by the action of communication means 47. These communication means 47 operate automatically and receives data which comprise control commands for starting time of delivery, the duration of the delivery and pressure level of the pressurized air I and/or starting time and the duration of the dosage of powder-like mixture S, K from the overall harvester control system 100 presented in FIG. 5.

[0073] In FIG. 5 is schematically illustrated a part of a forest harvester. This part of forest harvester comprises crane with a boom 7 and harvester head 6. Harvester head 6 comprises harvester head tilting device (not shown), feed rollers 61, delimbing knives 62, chain saw 5, stump treatments device for liquid (urea) and stump treatment device (spreading device) 1 for microbial propagules. The latter uses pneumatics circuit whereas the other devices, which are connected to harvester head, are controlled by hydraulic circuits. Each hydraulic circuit comprises hydraulic pump driven by an electric or a combustion motor (for example diesel motor) and proper valve, piping, filter and the actuator, Actuator can be hydraulic motor, hydraulic cylinder, electric motor or like which uses harvester head 1 device. Same hydraulic pump may provide hydraulic fluid to several harvester head 6 devices.

[0074] The hydraulic circuits of different device may be controlled in different way depending on the overall harvester control system 100. For example, overall hydraulic system 100 may employ only one hydraulic pump, which then gives power to crane 92, base machine, electricity 103 and also to working hydraulics 105 of the harvester head 6 devices. There may be overall hydraulic system 100 in which many device hydraulic circuits have their own hydraulic pumps; for example each delimbing knife 62 may have their own hydraulic motor and one hydraulic pump employ each knife hydraulic motor. However, we will consider here the most common harvester control system 100 which uses the same hydraulic pump to employ all working hydraulics 105 in a harvester head 6. Working hydraulics 105 comprises then following device controls controlled by corresponding hydraulic circuits, shown in FIG. 5: Chain saw 91, knives 92, harvester head tilt 94, feed rollers 95, stump treatment device for microbial propagules 96 and stump treatment device for liquid 97. Each hydraulic circuit for controlling corresponding harvester head 6 device includes hydraulic pump, electromagnetic valve and proper hydraulic fluid tubes or pipe leading hydraulic fluid to actuator (hydraulic motor hydraulic cylinder) connected to this specific device.

[0075] Fluid pipes leading to harvester head device may be provided by pressure gauges for measuring fluid pressure. Control system 100 may also comprise data measurements 80, for example trunk shape measurement 81, trunk diameter measurement 82 and trunk length measurement 83. The information (data) derived from data measurement(s) can be used in combination of data derived from hydraulic circuit of a harvester head 6 device to compose control command(s) for controlling other harvester head devices. For example data derived from chain saw 5, knives 92, harvester head tilt device 94, feed rollers 95, and stump treatment device for liquid 97 can be combined to data measurements 80 and this combined information can then be used for structuring a control command.

[0076] In the present invention it is used mainly those control commands which are derived from harvester control system 100 and which comprise control commands which relate to working hydraulics of the harvester head 6 and which specifically are derived from the hydraulics circuit 91 of the chain saw 5 or/and derived from the hydraulic circuit of the tree stump treatment device for liquid 97.

[0077] Control commands from chain saw hydraulic circuit 91 are generated by the electromagnetic valve(s) which open/close the fluid pressure connection between hydraulic pump and chain saw 5. Control commands derived from the hydraulic circuit 91 connected to the usage of chain saw 5 or/and usage of the tree stump treatment device for liquid 97 relate, for example to start/shut down chain saw motor (hydraulic motor) or to turn/swing the bar with saw chain into a saw box or out from the saw box. The saw box is connected to the harvester head 6 (see also FIG. 7).

[0078] Control commands from chain saw 5 hydraulic circuit 91 can be also generated by the pressure gauge in the pipe or tube of the hydraulic circuit 91 of the chain saw 5.

[0079] The measurement derived from the pressure gauge may indicate, for instance when the bar of the chain saw is turned back and forth when the standing tree is sawed and cutted at its lowermost part. This will result a tree stump T which can be treated with liquid urea, liquid colour markings or as in the present invention, with powder-like mixture.

[0080] Those control commands derived from chain saw 5 hydraulic circuit 91 and which relate to hydraulic actuators of employing chain saw (hydraulic motor or hydraulic cylinder) are then delivered by means of communication means 47 to control apparatus 43 for the dosage of the powder-like mixture (S, K) with the stepper motor 431. Same kind of control commands are also delivered by the action of communication means 47 to regulating means for controlling first and second electromagnetic valve 411a, 411b and pressure accumulator 42.

[0081] As mentioned before communication means 47 comprise preferable from wireless transmitter-receiver systems. This kind of wireless communication means 47 based on wireless technology is widely known and we refer here to existing literature on this field. Exemplary wireless communication means 47 are transmitter using electromagnetic signals transmitted from an antenna and a proper receiver of these signals (receptor),

[0082] As shown in FIG. 3 pressurized air is arranged to flow automatically inside the frame 2 and onward to the spreading end 3 from pressure accumulator 42 by means of two on/off electromagnetic valves 411.

[0083] Referring now also to FIG. 7, there has been schematically presented in a side view a chain saw 5, which is connected to a harvester head 6 of the forest machine from its saw box (not shown in figure). The harvester head 1 has been placed against the standing tree and chain saw 5 has been activated the. During the tree cutting operation the bar 51 with the saw chain 53 swings out from the saw box and cuts the tree forming a tree stump T. The tree is cutted so that the bar 5 moves in path P having starting point P2 and end point P1. At the end point of this path P tree has been cutted and began to fell down and the bar with the saw chain swings back to the saw box. The control commands from chain saw 5 hydraulic circuit 91 is generated from hydraulic actuators which controls the actions of chain saw 5 after the chain saw 5 have reached the starting P2 at its path P in cutting a standing tree. These actuators are for example hydraulic motor for using the saw chain 53 and and a hydraulic cylinder for swinging the bar. In the beginning of cutting tree, chain saw bar 51 is turned outside of the saw box connected to the harvester head 6.

[0084] Control command defining the beginning of the loading of accumulator 42 by opening the first electromagnetic valve 411a and also the starting point of the dosage of the powder-like mixture S, K containing microbial propagules and their possible culture medium S with the proper carrier K inside the frame 2 is generated by means of the control commands derived from chain saw 5 hydraulic circuit 91. This hydraulic circuit 91 controls the actuators which will be connected to actions of chain saw 5 when cutting the standing tree and forming a tree stump T.

[0085] In FIG. 7 it has been presented how bar 51 of the chain saw 5 reaches the end point P1 at its path P in sawing/cutting a tree. This event generates a new control command identifying the end of the loading of pressure accumulator 42 by closing the first electromagnetic valve 411a and end of dosage of powder-like mixture S, K inside frame by means of the control apparatus 43. The timing of the opening and closing each valve 411a, 411b depends on the diameter of the tree stump, which can be also measured in the beginning of the method, at the stage 110. However, as stated above the diameter of the tree stump T to be achieved, can also be derived from the actions of chain saw 5 when it goes in its path P from point P2 to point P1.

[0086] Control command defining the delivery time of the pressurized air I inside the frame 2 by opening the second electromagnetic valve 411b is generated by the control command which is identified or derived from the chain saw hydraulic circuit 91 when the tree began to fell and tree stump T will be formed. Because delivering/spreading of mixture of microbial propagules and their possible culture medium S in connection with the carrier K onto the tree stump T should be made immediately after the tree stump T has been formed or when the cut tree T is still felling down this event should be connected with turning of bar 51 of the chain saw 5 back in to saw box. This event can also be connected to a certain time delay from the release of control command(s) identifying the end of the loading of accumulator 42 by closing the first electromagnetic valve 411a. This means that the second electromagnetic valve 411b is opened only after some time delay has been elapsed from the dosage of powder-like mixture S, K inside the frame 2.

[0087] The above described advantageous embodiment of the present invention is also presented in FIG. 6B: Diameter of the tree stump T is measured or will be get from the actions of chain saw when sawing a tree, in the beginning of the method at the stage 110. Powder cartridge 452 containing a powder-like mixture S, K is brought into the storage 451 of a powder feeding device 45, which powder-like mixture comprises microbial propagules and a solid fine carrier, having a volumetric weight of at least 0.50 g/cm.sup.3, at least 0.6 g/cm.sup.3 or at least 1.0 g/cm.sup.3.

[0088] Aerosol A can then be formed from pressurized air I and powder-like mixture S, K. First is released/dosed the powder-like mixture S, K into the pressurized air I inside the frame 2 (stage 200). This is done by receiving and defining automatically data about administering time and duration of the powder-like mixture S, K from the hydraulic circuit control commands derived from chain saw 5 hydraulic circuit 91 and by transmitting these control commands to control means 43 (stage 200) which will dosage said powder-like mixture into frame by means of stepper motor 431. Due to small amount of powder-like mixture the stepper-motor should be provided with a suitable reduction gear.

[0089] Powder-like mixture S, K is first brought with powder cartridge 452 having a removable cap into the container 451 of the powder storage 45. Cap is removed from the cartridge 452 which may be tube made of plastic, cardboard or thin metal. Hollow frame 2 of the spreading device 1 can be connected into flow connection of the powder-like mixture S, K via a supply tube 453. From the container 451 the powder-like mixture S, K can be dispensed by means of stepper motor 431 along the supply tube 453 to the inside of frame 2.

[0090] The amount of powder-like mixture S, K depends preferably on the diameter of the tree stump, which is measured in the beginning of the method, at the stage 110.

[0091] After the powder-like mixture S, K have been dispensed inside the frame, the pressurized air I is brought inside the frame 2 along pressurized air I supply pipe 413. Aerosol A is formed (stage 600), as shown in FIG. 3, inside of frame 2 from the powder-like mixture S, K and the pressurized air I. This pressurized air I is first loaded into pressure accumulator 42 (stage 350) by opening the first electromagnetic valve 411a and then delivered inside the frame by closing the first electromagnetic valve 411 and opening the second electromagnetic valve 411b (stage 401). Control commands defining these valve actions and loading of pressure accumulator into a certain pressure level has been described above. Shortly said, these control commands are generated from chain saw 5 hydraulic circuit 91 and transmitted to valves 411a, 411b by means of communication means 47 (stage 301).

[0092] The flow or pressurized air to the inside of the frame 2 and further into spreading head 3 is controlled by first electromagnetic valve 411a which is located just after pressure accumulator 42 in the flow direction of pressurized air I. Aerosol A of dry matter formed on the inside of frame 2 is dispensed through application head 3 to the target, i.e. onto a tree stump T.

[0093] Spreading or dispersing the aerosol (A) (stage 700) to a tree stump is done by means of a pressurized air I released from the pressure accumulator 42.

[0094] Once aerosol A has been dispensed onto a tree stump through nozzles 31 of spreading head 3, the spores of Phiebiopsis gigantea contained in the powder-like mixture S, K begin to germinate, the fungal mycelium starts to occupy the tree stump T and prevent the growth of the root and butt rot on the tree stump.

[0095] Powder-like mixture S, K in powder cartridge 452 contains fungus spores as well culture medium S of these fungal spores as well as solid fine-grained carrier K. When the fungal spores are Phlebiopsis gigantea spores, their culture medium may be, for example, powdery silica gel. The fungal spores associated with the culture medium have a particle size of about 10 m and therefore the particle size K of the fine-grained carrier K should be of the same order of magnitude as the relatively dry powder S.

[0096] FIG. 4 further presents a screen chart showing the particle size distribution of a preferred fine-grained carrier K, i.e. lime filler. FIG. 4 presents on the vertical axis (Y axis) the pass-through percentage of powder passing the screen, and the size of openings of the screen respectively on the horizontal axis (x-axis). As seen from FIG. 4, the particle size of lime according to a screen analysis is approximately equal to the particle size of the culture medium and the fungal spores of Phlebiopsis gigantea, whereby a homogeneous, dry powdery mixture S, K can be formed of lime, culture medium (silica gel) and fungal spores Phlebiopsis gigantea.

[0097] Table 1 further shows volume weights of some powdery substances. Of these powdery substances, Rotstop contains silica gel and fungal spores, and ammonium lignosulphonate can be used as an additive in a powdery mixture. Other powder-like substances (such as, for example but not limited to, lime, gypsum, kaolin) shown in Table 1, could be used as a solid carrier K in a powder-like mixture according to the invention to transfer the dry powder formed by fungal spores and the culture medium onto the target, i.e. a tree stump. As shown in Table 1, the volume weight of lime is about 1.44 g/ml, so it is sufficiently heavy to carry the mixture of fungal spores and their culture medium to the target, i.e. the tree trunk. Gypsum and kaolin may also be used as a solid carrier K, although their volume weight is somewhat lower than that of lime (volume weight of phosphogypsum is 1.04 g/ml and of kaolin, 0.58 g/ml, compare Table 1). The volume weight of the solid carrier K should at least be approximately 0.50 g/ml, however preferably at least 1 g/ml. As can be seen, the Rotstop powder, which mainly contains fungal spores and the medium (silica gel) used to grow them, has a relatively low volume weight of only about 0.22 g/ml, whereby the spreading of a dry matter aerosol A onto a tree stump matter containing it alone could be troublesome, at least in windy conditions. It has further been demonstrated that Phiebiopsis gigantea colonizes well the stump surface, even without a liquid carrier, as there is enough moisture on the freshly cut stump surface to allow for this. However, to ensure a proper density of fungal spores across the stump surface, the total volume of powder applied has to be increased by adding a carrier.

TABLE-US-00001 TABLE 1 Densities of some ingredients contained in the powder-like mixture S, K. Material/density g/ml g/l Ammonium lignosulphonate 0.60 604 Filler lime (Nordkalk) 1.44 1442 Phosphogypsum (Siilinjrvi) 1.04 1043 Kaolin (standard porcelain) 0.58 583 Rotsop WP 0.22 220

[0098] Only some embodiments of the invention have been described above and it will be apparent to those skilled in the art that the invention can be implemented in many other ways within the scope of the inventive idea set forth in the claims.

[0099] Dispensing of aerosol A can be done for example by using a servo motor or like with a feedback instead of a stepping motor. Control valve 411 may in some cases also be a damper plate.

[0100] Besides fungal spores and their culture medium as well as fine-grained carrier substance K, the powdery mixture S, K contained in powder cartridge 452 may also preferably contain dye and supplemental nutrients. The supplemental nutrients are preferably selected from a group including lignin and its derivatives such as lignosulphonate and lignohumate and those will help increase the growth rate of fungal spores on a tree stump. In powdery mixture S, K the activity of the fungal spores remains at a high level significantly longer than in a solution-based product.

[0101] The powder-like mixture S, K blown onto the tree stump with aerosol A should be water-absorbent so that the fungal spores will adhere properly. Water absorption can be increased either by selecting a suitable water absorbing culture medium for fungal spores (for example silica gel) or a suitable fine-grained carrier.

EXAMPLE

[0102] Study Comparing the Efficacy of Liquid and Solid Formulation of Phlebiopsis gigantea Spores

[0103] The upper surfaces of freshly cut billets of Norway spruce were treated with powder containing Phlebiopsis gigantea (strain ATCC 90304) spores. Spores were obtained by cultivating Phlebiopsis gigantea on solid silica carrier and drying the colonized growth medium. Inert carriers, calcium carbonate and ammonium lignosulphonate, were mixed with the dry growth medium to obtain the following powder formulation:

[0104] 25% P. gigantea spores on silica

[0105] 65% CaCO.sub.3

[0106] 10% ammonium lignosulphonate

[0107] The surface of each billet (length about 30 cm, diameter 14-18 cm) was divided in two sectors, one section was treated with the powder mixture comprising the fungal spores and a root rot fungus Heterobasidion parviporum, and the second section was treated only with Heterobasidion parviporum.

[0108] Two powder application rates were studied, 1.0 and 2.0 mg/cm.sup.2. Control billets were treated with a commercial stump treatment agent, Rotstop SC, as a 1 g/l water suspension. The billets were kept in open air for 2.5 months. Then the billets were cut 5 cm below the surface and a 2 cm thick disc was taken, incubated in a plastic bag for one week and analyzed under a dissecting microscope for Heterobasidion covered area based on conidiophore development during the incubation. Only discs where an established Heterobasidion infection in the control side was observed were taken into account in calculation of the results.

TABLE-US-00002 TABLE 2 Area infested with Heterobasidion in spruce discs treated with different preparations containing Phlebiopsis gigantea in comparison with control discs with Heterobasidion only. Area treated with the product and Control area treated with Number Discs with no Hererobasidion Hererobasidion only Efficacy of discs Heterobasidion Total disc area Infested area Total disc area Infested area of pcs pcs % cm.sup.2 cm.sup.2 % cm.sup.2 cm.sup.2 % treatment Powder, 1 mg/cm2 21 16 76% 1428 1.62 0.113% 1488 20.53 1.38% 92% Powder, 2 mg/cm2 24 22 92% 1596 1.75 0.110% 1799 27.53 1.53% 94% Rotstop suspension 23 14 61% 1394 1.34 0.096% 1520 48.59 3.20% 97%

[0109] The results show that all the preparations containing Phiebiopsis gigantea controlled Heterobasidion root rot fungus very effectively. The efficacy was over 90% with all the treatments. Efficacy of the treatment with the powder formulation was comparable to the treatment with the commercial stump treatment method which is in an aqueous formulation.

REFERENCE NUMBER LIST

[0110] 1 Spreading device [0111] 2 Frame [0112] 21 measurement means [0113] 3 spreading head [0114] 31 nozzles [0115] 4 Administering means [0116] 41 regulating means [0117] 411 regulating valve(s) [0118] 411a first electromagnetic valve [0119] 411b second electromagnetic valve [0120] 413 (pressurized) air supply pipe [0121] 42 pressure accumulator [0122] 43 control apparatus [0123] 431 stepper motor [0124] 45 powder storage [0125] 451 container [0126] 452 powder cartridge [0127] 453 supply tube [0128] 47 communication means [0129] 5 Chain saw [0130] 51 bar [0131] 52 bar holder [0132] 53 chain [0133] 6 Harvester head [0134] 7 Boom [0135] 80 Data measurement [0136] 81 trunk shape [0137] 82 trunk diameter [0138] 83 trunk diameter [0139] 90 Hydraulic circuit [0140] 91 chain saw [0141] 92 crane, boom [0142] 93 knives [0143] 94 harvester head tilt [0144] 95 feed rollers [0145] 96 stump treatment device for microbial propagules [0146] 97 stump treatment device for liquid [0147] 100 Harvester control system [0148] 101 crane hydraulics [0149] 102 pneumatics [0150] 103 electricity [0151] 105 working hydraulics for harvester head [0152] I Compressed air [0153] K Carrier [0154] P Path of the bar [0155] P1 End point of the path [0156] P2 Starting point of the path [0157] S Mixture of microbial propagules and their culture medium [0158] S1 Mixture of fungal spores and their culture medium [0159] S, K Powder-like mixture [0160] T Tree stump

[0161] Following are particular embodiments of the disclosed invention.

[0162] E1. Spreading device (1) for administering onto a tree stump a composition which comprises microbial propagules to treat or prevent pathogen, which spreading device has a spreading end (3) connected to a pipe-like frame (2), and administering means (4) for administering said composition, characterized in that the administering means (4) comprise a feeding device (42) for pressurized air connected to the first end of the spreading device's (1) frame (2) and regulating means (41) for regulating the volumetric flow of pressurized air (I) entering the frame, a powder storage (45) in contact with the frame (2), which storage contains a powder-like mixture (S, K) made up of microbial propagules and a solid fine carrier (K), and a regulating device (43) connected to the second end of the frame (2) for administering the powder-like mixture (S, K) inside the frame (2) and means for blowing the powder-like mixture (S, K) onto a tree stump, via the spreading end (3) connected to the frame (2).

[0163] E2. The spreading device (1) according to claim 1, characterized in that powder-like mixture (S, K) comprises microbial propagules which prevents the growth of Heterobasidion.

[0164] E3. The spreading device (1) according to claim 1 or 2, characterized in that powder-like mixture (S, K) is made up of microbial propagules, its possible culture medium (S) and a solid fine carrier (K).

[0165] E4. The spreading device (1) according to any of the claims 1-3, characterized in that microbial propagules is made up of fungal spores.

[0166] E5. The spreading device (1) according to claim 1, characterized in that the powder storage (45) comprises a container (451), which contains a powder-like mixture (S, K), and means (453) for bringing said powder-like mixture (S, K) inside the frame (2).

[0167] E6. The spreading device (1) according to claim 1, characterized in that the regulating means (41) of the administering means (4) comprise a regulating valve (411) for altering the volumetric flow of pressurized air (I) entering the frame (2).

[0168] E7. The spreading device (1) according to claim 6, characterized in that the regulating valve (411) comprises a magnetic valve (411a) and/or a damper.

[0169] E8. The spreading device (1) according to claim 1, characterized in that the regulating device (43) comprises a stepper motor (431) or a servo motor.

[0170] E9. The spreading device (1) according to claim 1, characterized in that the powder storage (45) comprises a container (451), on the inside of which a powder cartridge (452) can be installed, which cartridge (452) comprises a powder-like mixture of microbial propagules, and a fine solid carrier (K).

[0171] E10. The spreading device (1) according to claim 9, characterized in that the container (451) is connected to the inside of the frame (2) via a feeding pipe (453).

[0172] E11. The spreading device (1) according to claim 9, characterized in that powder cartridge (452) comprises a powder-like mixture (S, K), made up of microbial propagules, its culture medium (S) and a solid fine carrier (K).

[0173] E12. The spreading device (1) according to any of claim 9-11, characterized in that microbial propagules is made up of fungal spores.

[0174] E13. The spreading device (1) according to claim 11 or 12, characterized in that the fungal spores' culture medium comprises silica gel.

[0175] E14. The spreading device (1) according to claim 12 or 13, characterized in that fungal spores comprises spores of Phlebiopsis gigantea.

[0176] E15. The spreading device (1) according to any of claims 9-14, characterized, the volumetric weight of solid fine (K) carrier is at least 0.50 g/ml, preferably at least 0.60 g/ml most preferably at least 1 g/ml.

[0177] E16. The spreading device (1) according to any of claims 9-15, characterized in that the powder cartridge (452) contains a solid fine carrier, which is selected from the group consisting of lime (CaCO.sub.3), plaster and kaolin.

[0178] E17. The spreading device (1) according to any of the claims 9-16, characterized in that the powder cartridge (452) additionally contains a colouring substance and nutritional supplements.

[0179] E18. A powder cartridge (452) available for use in a feeding device (45) of a spreading device (1) according to claims 1 and 9, characterized in that the powder cartridge (452) contains a powder-like mixture, which comprises microbial propagules and a solid fine carrier, the characteristic weight of which is at least 0.5 g/ml, preferably at least 0.6 g/ml most preferably 1.0 g/ml.

[0180] E19. The powder cartridge (452) according to claim 18, characterized in that the solid fine carrier (S) is selected from a group consisting of lime, plaster and kaolin.

[0181] E20. The powder cartridge (452) according to claim 18 or 19, characterized in that the powder-like mixture additionally contains a colouring substance and nutritional supplements.

[0182] E21. The powder cartridge (452) according to claim 20, characterized in that the nutritional supplement comprising lignin and its derivatives, such as lignosulphonate and lignohumate.

[0183] E22. The powder cartridge (452) according to claim 18-24, characterized in that the powder cartridge contains 5-30 w-% of a mixture of microbial propagules, 60-90 w-% solid carrier and 1-10 w-% colouring substance and nutritional supplements.

[0184] E23. The powder cartridge (452) according to any of the claims 18-22, characterized in that the microbial propagules are fungal spores which prevents the growth of Heterobasidion.

[0185] E24. The powder cartridge (452) according to claim 23, characterized in that the fungal spores are spores of Phiebiopsis gigantean and said powder cartridge contains additionally the culture medium.

[0186] E25. The powder cartridge (452) according to claim 24, characterized in that the fungal spores' culture medium comprises silica gel.

[0187] E26. The powder cartridge (452) according to any of the claims 18-25, characterized in that the powder cartridge contains 70 w-% lime, 20 w-% of a mixture of Phiebiopsis gigantea fungal spores and their culture medium (S), and 10 w-% colouring substance and nutritional supplements.

[0188] E27. A composition in the form of a powder-like mixture (S, K), which comprises microbial propagules and a solid carrier, the volumetric weight of which is at least 0.50 g/ml, preferably at least 0.60 g/ml most preferably at least 1.0 g/ml.

[0189] E28. The composition according claim 27, characterized in that the powder-like mixture (S, K) contains a solid carrier, which is selected from the group consisting of lime, (CaCO.sub.3), plaster and kaolin.

[0190] E29. The composition according to claim 27 or 28, characterized in that the powder-like mixture (S, K) additionally contains a colouring substance and nutritional supplements.

[0191] E30. The composition according to claim 29, characterized in that the nutritional supplement comprises lignin and its derivatives, such as lignosulphonate and lignohumate.

[0192] E31. The composition according to any of the claims 27-30, characterized in that the microbial propagules are fungal spores which prevents the growth of Heterobasidion.

[0193] E32. The composition according to claim 31, characterized in that the fungal spores are spores of Phiebiopsis gigantean which composition contains additionally the culture medium.

[0194] E33. The composition according to claim 32, characterized in that the fungal spores' culture medium comprises silica gel.

[0195] E34. The composition according to any of the claims 27-33, characterized in that it contains 5-30 w-% of a mixture of Phiebiopsis gigantea fungal spores and their culture medium (S), 60-90 w-% solid carrier (K) and 1-10 w-% colouring substance and nutritional supplements.

[0196] E35. The composition according to any of claims 27-34, characterized in that the composition contains 70 w-% lime, 20 w-% of a mixture of Phiebiopsis gigantea fungal spores and their culture medium (S), and 10 w-% colouring substance and nutritional supplements.

[0197] E36. Use of any composition according to any one of the claims 27-35, which comprise a powder-like mixture (S, K) for treating pathogens or preventing growth of pathogens.

[0198] E37. The use of claim 36 wherein pathogen is fungal pathogen.

[0199] E38. The use of claim 36 wherein pathogen is Heterobasidon.

[0200] E39. Use of a composition comprising a powder-like mixture (S, K) according to any of the claims 27-35 for treating pathogens or preventing growth of pathogens on a tree stump, which use includes the spreading or dispersing of the powder-like mixture on said tree stump by machine or manually.

[0201] E40. The use of claim 39 wherein pathogen is fungal pathogen.

[0202] E41. The use of claim 40 wherein pathogen is Heterobasidon.

[0203] E42. The use according to any one of claims 39-41 wherein 250-1000 g of the powder-like mixture is spread onto the tree stumps per hectare of logging area.

[0204] E43. A method for spreading or dispersing onto a tree stump a powder-like mixture comprising microbial propagules and a solid fine carrier, the volumetric weight of which is at least 0.50 g/ml, more preferably 0.6 g/ml most preferably 1.0 g/ml, wherein the method comprises the following steps: [0205] generating an aerosol (A) containing a solid matter from pressurized gas and a powder-like mixture, which comprises microbial propagules and a solid fine carrier, the characteristic weight of which carrier is at least 0.50 g/ml, more preferably 0.6 g/ml most preferably 1.0 g/ml, [0206] spreading or dispersing the aerosol (A) containing solid matter to a tree stump by means of a pressurized gas.

[0207] E44. The method according to claim 43, characterized in that the pressurized gas is pressurized air (I).

[0208] E45. The method according to claim 43 or 44 for spreading or dispersing onto a tree stump a powder-like mixture comprising microbial propagules and a solid fine carrier, the characteristic weight of which carrier is at least 0.50 g/ml, more preferably 0.6 g/ml most preferably 1.0 g/ml, with a spreading device (1) defined in claim 1, characterized in that the method comprises the following steps: [0209] bringing a powder cartridge (452) containing a powder-like mixture into the storage (451) of a powder feeding device (45), which powder-like mixture comprises, in addition to microbial propagules a solid fine carrier (K), the characteristic weight of which is at least 0.50 g/ml, more preferably 0.6 g/ml most preferably 1.0 g/ml, [0210] manufacturing an aerosol (A) containing solid matter from pressurized air (I) and the powder-like mixture, by releasing the powder-like mixture into the pressurized air (I) inside the frame (2), [0211] spreading or dispersing the aerosol (A) to the tree stump by means of the pressurized air (I) contained in the aerosol (A).

[0212] E46. The method according to any of the claims 43-45, characterized in that the method further comprises arranging pressurized air (I) to flow inside the frame (2) and onward to the spreading end (3) by means of the regulating means (41) for pressurized air.

[0213] E47. The method according to any of the claims 43-46, characterized in that the method comprises administering the powder-like mixture into the pressurized air (I) inside the frame (2) by means of the regulating device (43).

[0214] E48. The method according to any of the claims 43-47, characterized in that the microbial propagules are fungal spores which prevents the growth of Heterobasidion.

[0215] E49. The method according to claim 48, characterized in that the fungal spores are spores of Phiebiopsis gigantean and said powder cartridge contains additionally the culture medium.

[0216] E50. The method according to claim 49, characterized in that the fungal spores' culture medium comprises silica gel.

[0217] E51. The method of claim 50, characterized in that fungal spores are spores of Phlebiopsis gigantean with possible culture medium.

[0218] E52. A powder-like mixture spread or dispersed onto a tree stump, which comprises fungal spores and culture medium of Phiebiopsis gigantea (S) and additionally a solid fine carrier (K), which powder-like mixture has absorbed water so that its characteristic weight has grown compared to the characteristic weight of the powder-like mixture contained in the powder cartridge (452).