TRADITIONAL CHINESE MEDICINE PLANT COMPOSITION, PREPARATION METHOD THEREFOR AND USE THEREOF

Abstract

A traditional Chinese medicine plant composition, comprising in parts by weight: 20-60 parts of Astragalus membranacus, 10-30 parts of Radix sileris, 10-30 parts of Rhizoma gastrodiae, 5-20 parts of Calendula officinalis, and 5-15 parts of Albizzia julibrissin. The traditional Chinese medicine plant composition has functions of relieving allergies, anti-Allergy itching, eliminating redness and swelling, and allergy recovery, and is safe and does not cause irritation, can be used in skin care products. The traditional Chinese medicine plant composition is prepared by crushing plant raw materials and then extracting same.

Claims

1.-10. (canceled)

11. A traditional Chinese medicine plant composition, comprising in parts by weight: 20-60 parts of Astragalus membranacus, 10-30 parts of Radix sileris, 10-30 parts of Rhizoma gastrodiae, 5-20 parts of Calendula officinalis, and 5-15 parts of Albizzia julibrissin.

12. The traditional Chinese medicine plant composition according to claim 11, comprising in parts by weight: 30-50 parts of Astragalus membranacus, 15-25 parts of radix sileris, 15-25 parts of Rhizoma gastrodiae, 8-15 parts of Calendula officinalis, and 5-10 parts of Albizzia julibrissin.

13. The traditional Chinese medicine plant composition according to claim 11, comprising in parts by weight: 35-45 parts of Astragalus membranacus, 18-25 parts of radix sileris, 18-25 parts of Rhizoma gastrodiae, 12-15 parts of Calendula officinalis, and 6-10 parts of Albizzia julibrissin.

14. A preparation method of the traditional Chinese medicine plant composition according to claim 11, comprising the following steps: (1) crushing plant raw materials and then evenly blending according to formula amounts. (2) extracting the blended raw materials obtained in the step (1) by reflux with 95% ethanol, cooling to below 30 C., and conducting vacuum suction filtration; (3) evaporating the suction-filtered mixture obtained in the step (2), adding 1,3-butanediol, cooling to below 30 C., and conducting vacuum suction filtration; (4) sterilizing the suction-filtered mixture obtained in the step (3), cooling to below 30 C. to prepare the traditional Chinese medicine plant composition.

15. The preparation method according to claim 14, wherein the crushing in the step (1) is aimed at a mesh count of 6-200 mesh.

16. The preparation method according to claim 15, wherein the mass to volume ratio of the raw materials to the ethanol in the step (2) is 1:(8-30).

17. The preparation method according to claim 15, wherein the temperature for the extraction by reflux in the step (2) is 80-85 C.

18. The preparation method according to claim 15, wherein the time for the extraction by reflux in the step (2) is 90-120 min.

19. The preparation method according to claim 14, wherein the evaporation in the step (3) is conducted by means of rotary evaporation.

20. The preparation method according to claim 19, wherein the temperature for the rotary evaporation is 40-80 C.

21. The preparation method according to claim 19, wherein the mass ratio of the filtered section to the 1,3-butanediol is 1:(0.5-3).

22. The preparation method according to claim 14, wherein the temperature for sterilization in the step (4) is 80-100 C.

23. The preparation method according to claim 22, wherein the time for the sterilization in the step (4) is 20-60 min.

24. A skin care product, comprising the traditional Chinese medicine plant composition according to claim 11.

25. The skin care product according to claim 24, wherein the traditional Chinese medicine plant composition accounts for 0.5-10% by mass of the skin care product.

26. The skin care product according to claim 24, further comprising an adjuvant; wherein the adjuvant includes an adjuvant phase A, an adjuvant phase B, and an adjuvant phase C; wherein the adjuvant phase A is xanthan gum and/or glyceryl; wherein the adjuvant phase B is a mixture of glyceryl stearate/PEG-100 stearate, cetearyl alcohol, pentaerythritol distearate, hydrogenated polyisobutylene, caprylic/capric triglyceride, poly dimethyl silicon and isostearyl isostearate; wherein the adjuvant phase C is a mixture of methyl isothiazolinone/iodopropynyl butylcarbamate and phenoxyethanol/ethylhexylglycerin.

27. The skin care product according to claim 26, wherein the adjuvant phase A comprises in percentages by mass: 0.02-0.08% of xanthan gum and 1.5-3% of glyceryl; TABLE-US-00011 glyceryl stearate/PEG-100 stearate 2-3% cetearyl alcohol 0.5-1.5% pentaerythritol distearate 1-2% hydrogenated polyisobutylene 2-4% caprylic/capric triglyceride 2-4% poly dimethyl silicon 1-3% isostearyl isostearate 2-4%; TABLE-US-00012 methyl isothiazolinone/iodopropynyl butylcarbamate 0.02-0.08% phenoxyethanol/ethylhexylglycerin 0.2-0.8%;

28. The skin care product according to claim 26, wherein the skin care product comprises in percentages by mass: the adjuvant phase A: TABLE-US-00013 the adjuvant phase A: xanthan gum 0.02-0.08% glyceryl 1.5-3%.sup. the adjuvant phase B: glyceryl stearate/PEG-100 stearate 2-3% cetearyl alcohol 0.5-1.5% pentaerythritol distearate 1-2% hydrogenated polyisobutylene 2-4% caprylic/capric triglyceride 2-4% poly dimethyl silicon 1-3% isostearyl isostearate 2-4% the adjuvant phase C: methyl isothiazolinone/iodopropynyl butylcarbamate 0.02-0.08% phenoxyethanol/ethylhexylglycerin 0.2-0.8%; the traditional Chinese medicine plant composition 0.5-10%; with the balance being water.

29. The preparation method of a skin care product according to claim 26, comprising the following steps: (1) adding the xanthan gum in the adjuvant phase A to the glyceryl and evenly blending, and heating to 80-85 C.; (2) mixing the raw materials of the adjuvant phase B, and heating to 80-85 C.; (3) adding all the adjuvant phase B obtained in the step (2) to adjuvant phase A obtained in the step (1), homogenizing at 2000-3000 r/min for 5-10 min, heating to 80 C. and stirring for 20-40 min, then starting cooling by stirring at a stirring rate of 30-50 r/min with cooling rate of 1-2 C./min; and (4) when the temperature is lowered to 45-50 C., adding the adjuvant phase C and the traditional Chinese medicine plant composition according to claim 11, stirring evenly, and continuing to cool to below 38 C. to obtain the skin care product.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0064] FIG. 1 shows the effect of the traditional Chinese medicine plant composition on the itching threshold of guinea pigs tolerant to histamine phosphate, wherein ** indicates the analysis by SPSS Dunnett-t test is employed;

[0065] FIG. 2 shows the effect of the traditional Chinese medicine plant composition on the capillary permeability of guinea pigs, wherein * and ** respectively indicate the analysis by SPSS Dunnett-t test is employed;

[0066] FIG. 3 shows the effect of the traditional Chinese medicine plant composition on the skin water loss of guinea pigs, wherein ## and ** respectively indicate the analysis by SPSS Dunnett-t test is employed;

[0067] FIG. 4 shows the improvement of sensitive skin by the skin care product prepared from the traditional Chinese medicine plant composition of the present invention in five dimensions.

DETAILED DESCRIPTION

[0068] In order to facilitate the understanding of the present invention, some examples are set forth herein below. Those skilled in the art shall understand that the embodiments are set forth to assist in understanding the present invention and should not be regarded as specific limitations to the present invention.

Example 1

[0069] A preparation method of the traditional Chinese medicine plant composition, comprising the following steps:

[0070] (1) 40 parts of Astragalus membranaceus, 20 parts of Radix sileris, 20 parts of Rhizoma gastrodiae, 12 parts of Calendula officinalis, and 8 parts of Albizzia julibrissin as plant raw materials were crushed to 50 mesh and blended evenly;

[0071] (2) the blended raw materials according to step (1) were reflux-extracted at a mass-to-volume ratio of 1:13 to 95% ethanol for heat reflux extraction at 80 C. for 120 min, cooled to below 30 C., and subjected to vacuum suction filtration;

[0072] (3) the filtered section obtained in the step (2) was injected to a rotary evaporator flask, and all the ethanol was rotary evaporated at 55 C., 1,3-butanediol was added in a mass ratio of 1:1 to the mixture, cooled to below 30 C., and subjected to vacuum suction filtration;

[0073] (4) the filtered section obtained in the step (3) was heat sterilized with a water-bath pot at 87 C. for 30 min, cooled to below 30 C. to prepare the traditional Chinese medicine plant composition.

Example 2

[0074] A preparation method of the traditional Chinese medicine plant composition, comprising the following steps:

[0075] (1) 35 parts of Astragalus membranaceus, 18 parts of Radix sileris, 18 parts of Rhizoma gastrodiae, 12 parts of Calendula officinalis, and 6 parts of Albizzia julibrissin as plant raw materials were crushed to 80 mesh and blended evenly;

[0076] (2) the blended raw materials according to step (1) were reflux-extracted at a mass-to-volume ratio of 1:10 to 95% ethanol for heat reflux extraction at 82 C. for 100 min, cooled to below 30 C., and vacuum subjected to suction filtration; (3) the filtered section obtained in the step (2) was injected to a rotary evaporator flask, and all the ethanol was rotary evaporated at 50 C., 1,3-butanediol was added in a mass ratio of 1:2 to the mixture, cooled to below 30 C., and subjected to vacuum suction filtration;

[0077] (4) the filtered section obtained in the step (3) was heat sterilized with a water-bath pot at 85 C. for 50 min, cooled to below 30 C. to prepare the traditional Chinese medicine plant composition.

Example 3

[0078] A preparation method of the traditional Chinese medicine plant composition, comprising the following steps:

[0079] (1) 45 parts of Astragalus membranaceus, 25 parts of Radix sileris, 25 parts of Rhizoma gastrodiae, 15 parts of Calendula officinalis, and 10 parts of Albizzia julibrissin as plant raw materials were crushed to 10 mesh and blended evenly; [0080] (2) the blended raw materials according to step (1) were reflux-extracted at a mass-to-volume ratio of 1:20 to 95% ethanol for heat reflux extraction at 85 C. for 120 min, cooled to below 30 C., and subjected to vacuum suction filtration;

[0081] (3) the filtered section obtained in the step (2) was injected to a rotary evaporator flask, and all the ethanol was rotary evaporated at 80 C., 1,3-butanediol was added in a mass ratio of 1:3 to the mixture, cooled to below 30 C., and subjected to vacuum suction filtration;

[0082] (4) the filtered section obtained in the step (3) was heat sterilized with a water-bath pot at 100 C. for 20 min, cooled to below 30 C. to prepare the traditional Chinese medicine plant composition.

Example 4

[0083] A preparation method of the traditional Chinese medicine plant composition, comprising the following steps:

[0084] (1) 30 parts of Astragalus membranaceus, 15 parts of Radix sileris, 15 parts of Rhizoma gastrodiae, 10 parts of Calendula officinalis, and 5 parts of Albizzia julibrissin as plant raw materials were crushed to 100 mesh and blended evenly;

[0085] (2) the blended raw materials according to step (1) were reflux-extracted at a mass-to-volume ratio of 1:10 to 95% ethanol for heat reflux extraction at 82 C. for 100 min, cooled to below 30 C., and subjected to vacuum suction filtration;

[0086] (3) the filtered section obtained in the step (2) was injected to a rotary evaporator flask, and all the ethanol was rotary evaporated at 50 C., 1,3-butanediol was added in a mass ratio of 1:1 to the mixture, cooled to below 30 C., and subjected to vacuum suction filtration;

[0087] (4) the filtered section obtained in the step (3) was heat sterilized with a water-bath pot at 85 C. for 40 min, cooled to below 30 C. to prepare the traditional Chinese medicine plant composition.

Example 5

[0088] A preparation method of the traditional Chinese medicine plant composition, comprising the following steps:

[0089] (1) 20 parts of Astragalus membranaceus, 10 parts of Radix sileris, 10 parts of Rhizoma gastrodiae, 5 parts of Calendula officinalis, and 5 parts of Albizzia julibrissin as plant raw materials were crushed to 6 mesh and blended evenly;

[0090] (2) the blended raw materials according to step (1) were reflux-extracted at a mass-to-volume ratio of 1:8 to 95% ethanol for heat reflux extraction at 85 C. for 120 min, cooled to below 30 C., and subjected to vacuum suction filtration;

[0091] (3) the filtered section obtained in the step (2) was injected to a rotary evaporator flask, and all the ethanol was rotary evaporated at 40 C., 1,3-butanediol was added in a mass ratio of 1:0.5 to the mixture, cooled to below 30 C., and subjected to vacuum suction filtration;

[0092] (4) the filtered section obtained in the step (3) was heat sterilized with a water-bath pot at 100 C. for 20 min, cooled to below 30 C. to prepare the traditional Chinese medicine plant composition.

Example 6

[0093] A preparation method of the traditional Chinese medicine plant composition, comprising the following steps:

[0094] (1) 60 parts of Astragalus membranaceus, 30 parts of Radix sileris, 30 parts of Rhizoma gastrodiae, 20 parts of Calendula officinalis, and 15 parts of Albizzia julibrissin as plant raw materials were crushed to 200 mesh and blended evenly;

[0095] (2) the blended raw materials according to step (1) were reflux-extracted at a mass-to-volume ratio of 1:30 to 95% ethanol for heat reflux extraction at 80 C. for 90 min, cooled to below 30 C., and subjected to vacuum suction filtration;

[0096] (3) the filtered section obtained in the step (2) was injected to a rotary evaporator flask, and all the ethanol was rotary evaporated at 80 C., 1,3-butanediol was added in a mass ratio of 1:3 to the mixture, cooled to below 30 C., and subjected to vacuum suction filtration;

[0097] (4) the filtered section obtained in the step (3) was heat sterilized with a water-bath pot at 80 C. for 60 min, cooled to below 30 C. to prepare the traditional Chinese medicine plant composition.

Comparison Example 1

[0098] The same as Example 1 except for no Astragalus membranaceus, 30 parts of radix sileris, 30 parts of Rhizoma gastrodiae, 20 parts of Calendula officinalis, and 20 parts of Albizzia julibrissin.

Comparison Example 2

[0099] The same as Example 1 except for no Radix sileris, 50 parts of Astragalus membranaceus, 30 parts of Rhizoma gastrodiae.

Comparison Example 3

[0100] The same as Example 1 except for no Rhizoma gastrodiae, 50 parts of Astragalus membranaceus, 30 parts of Radix sileris.

Comparison Example 4

[0101] The same as Example 1 except for no Calendula officinalis, 52 parts of Astragalus membranaceus.

Comparison Example 5

[0102] The same as Example 1 except for no Albizzia julibrissin, 48 parts of Astragalus membranaceus.

Comparison Example 6

[0103] The same as Example 1 except for 15 parts of Astragalus membranaceus, 5 parts of Radix sileris, 5 parts of Rhizoma gastrodiae, 3 parts of Calendula officinalis, and 3 parts of Albizzia julibrissin.

Comparison Example 7

[0104] The same as Example 1 except for 65 parts of Astragalus membranaceus, 35 parts of Radix sileris, 35 parts of Rhizoma gastrodiae, 25 parts of Calendula officinalis, and 20 parts of Albizzia julibrissin.

[0105] The relevant tests were carried out on Examples 1-6, Comparative Examples 1-7 and commercially available products, and the tests are as follows:

[0106] (1) Anti-Allergy Itching-Inhibiting Itching Caused by Histamine

[0107] Histamine, which acts as a chemical delivery substance in mast cells, plays an important role in the development of anaphylactic diseases or inflammation in the body. Itching is a symptom of cutaneous anaphylaxis and is also associated with the release of histamine by local mast cells, accordingly, the model, where endogenous histamine released by inducing with exogenous histamine causes skin itching, can be used to test the abilities of the compositions to render animals resistant to histamine, and to further evaluate the antipruritic and anti-allergic effects of the traditional Chinese medicine plant compositions.

[0108] Histamine phosphate that has a higher itching threshold indicates that it has a better anti-allergic effect.

[0109] One day before the test, the samples were applied to the right hind paw dorsum of each group of guinea pigs once. On the day of the test, a shaved portion of the right hind paw of the animal was abraded with a coarse sandpaper by about 1 cm.sup.2, and the samples were applied thereto once, while the blank control group was given an equal amount of distilled water. After 10 min, the corresponding concentration of histamine phosphate was added dropwise at the abrasion, and then the concentration was incremented sequentially increased every 3 min until the guinea pig turned back to lick its right hind paw, and the total amount of histamine phosphate dropped when finally the guinea pig turned back to lick its right hind paw was considered as the itching threshold.

[0110] The results are shown in FIG. 1 and Table 1. In FIG. 1, Example 1 was used as the test product group, and the test product group was applied with different concentrations of 1%, 2%, 5% and 10%, and the test comprises blank control group and positive control group (dexamethasone acetate), after applying the test product to the itching site, the traditional Chinese medicine plant composition with a concentration of 5% and 10% can significantly increase the itching threshold of histamine phosphate in guinea pigs, which has a significant difference when compared with the blank in the control group (P<0.01), moreover, the itching threshold is proportional to the addition amount of the traditional Chinese medicine plant composition.

[0111] In Examples 1-6 and Comparative Examples 1-7 in Table 1, the traditional Chinese medicine plant composition was applied to the itching site at a concentration of 5%, and the results are shown in Table 1:

TABLE-US-00004 TABLE 1 Itching threshold of histamine phosphate (g) Example 1 57 Example 2 55 Example 3 53 Example 4 50 Example 5 48 Example 6 47 Comparison Example 1 26 Comparison Example 2 30 Comparison Example 3 31 Comparison Example 4 32 Comparison Example 5 33 Comparison Example 6 35 Comparison Example 7 37

[0112] As can be seen from Table 1, compared with Comparison Examples 1-7, the itching threshold of histamine phosphate was greatly improved Examples 1-6 by adjusting the ratio of the five components of Astragalus membranacus, Radix sileris, Rhizoma gastrodiae, Calendula officinalis and Albizzia julibrissin; in addition, compared with Comparison Examples 4-6, the itching threshold of histamine phosphate was further improved in Examples 1-3 by further controlling the weight fractions of Astragalus membranacus, Radix sileris, Rhizoma gastrodiae, Calendula officinalis and Albizzia julibrissin.

[0113] (2) Eliminating Redness and Swelling-Decreasing Capillary Permeability

[0114] The antiallergic function of the actives was investigated by a passive skin allergy model. The antibody and the antigen were combined to to cause type I hypersensitivity reaction, resulting in a significant increase in local capillary permeability and the appearance of allergic symptoms such as redness and swelling. It is currently possible to simulate this process in rats, which is an ideal method for studying the antiallergic function of active substances in vivo.

[0115] The rat skin, where the type I allergic reaction had occurred and Evans Blue dye was contained, was locally treat by using the traditional Chinese medicine plant composition, so that the function of inhibiting capillary permeability was achieved via the antiallergic function, and thereby the dye content of the local skin was reduced. The dye content was calculated by spectrophotometric method and compared with the model control group (with deionized water instead of the sample) to examine the decrease rate of capillary permeability contributed by the sample.

[0116] The decrease rate of capillary permeability by % was calculated according to the formula: capillary permeability reduction rate by %/=(T.sub.0T.sub.n)/T.sub.0*100, where T.sub.0 is the dye content of the model control group, and T.sub.n is the dye content of the sample group. The higher decrease rate of capillary permeability indicates the more pronounced inhibition of allergic response.

[0117] The results are shown in FIG. 2 and Table 2. As can be seen from FIG. 2, the traditional Chinese medicine plant composition can significantly reduce the capillary permeability of the rat skin during the allergic reaction, effectively alleviate the allergy symptoms, and is positively correlated with the weight concentration, 1%, 5% of Chinese medicine plant composition showed significant difference (p<0.05) compared with the blank control group (formulation matrix, without the Chinese medicine plant composition), wherein 10% of the traditional Chinese medicine plant composition showed extremely significant difference (p<0.01).

[0118] In Examples 1-6 and Comparison Examples 1-7 in Table 2, the traditional Chinese medicine plant composition was applied to the itching site at a concentration of 5%, and the results are shown in Table 2:

TABLE-US-00005 TABLE 2 Decrease rate of capillary permeability Example 1 16 Example 2 15 Example 3 14 Example 4 10 Example 5 11 Example 6 10 Comparison Example 1 3 Comparison Example 2 4 Comparison Example 3 3 Comparison Example 4 5 Comparison Example 5 4 Comparison Example 6 6 Comparison Example 7 5

[0119] As can be seen from Table 2, compared with Comparison Examples 1-7, the decrease rate of capillary permeability was greatly improved Examples 1-6 by adjusting the ratio of the five components of Astragalus membranacus, Radix sileris, Rhizoma gastrodiae, Calendula officinalis and Albizzia julibrissin; in addition, compared with Comparison Examples 4-6, the decrease rate of capillary permeability was further improved in Examples 1-3 by further controlling the weight fractions of Astragalus membranacus, Radix sileris, Rhizoma gastrodiae, Calendula officinalis and Albizzia julibrissin.

[0120] (3) Allergy Recovery-Decreasing Skin Water Loss after Allergy

[0121] When the skin barrier was damaged by an allergic reaction, the skin moisture would be largely lost; a model of guinea pig skin injury and dehydration was used to simulate the skin water loss after allergy so as to evaluate the functions of allergy recovery and barrier consolidation and protection of the traditional Chinese medicine plant composition. The traditional Chinese medicine plant composition prepared in Example 1 was used in the test, and the composition at a concentration of 1% and 5% was applied to the local damaged skin of the guinea pigs at a dose of 0.1 mL/cm.sup.2, the change of the water loss of the damaged skin was detected by using a water loss tester, the difference between the groups was compared, and the water loss protection rate was calculated. The laboratory apparatus was: Tewameter TM300, available from CK Company, Germany.

[0122] The water loss protection rate by % was calculated according to the formula: water loss protection rate by %/=(T.sub.mT.sub.n)/(T.sub.mT.sub.c)*100, where T.sub.m is the data collection value of the water loss of the model control group (deionized water instead of the sample), T.sub.n is the data collection value of the water loss of the sample group, and T.sub.c is the data collection value of the blank control group (no dehydration treatment, with deionized water instead of the sample). The higher water loss protection rate decrease rate indicates the more pronounced barrier repairing effect after allergy.

[0123] The test results are shown in FIG. 3 and Tables 3-4.

TABLE-US-00006 TABLE 3 Skin water loss Water loss Groups (g/hm.sup.2)) protection rate (%) Blank control group 13.0 0.4 Model control group 20.0 0.4 Commercially available competitor 19.5 0.5 7 (amount: 5%) 1% addition 15.1 0.4 70 5% addition 14.7 0.3 76

[0124] It can be seen from FIG. 3 and Table 3 that the traditional Chinese medicine plant composition can significantly reduce the water loss of the damaged skin of the guinea pig, effectively repair the skin barrier, and these effects are positively correlated with the weight concentration, and have a significant difference (p<0.01) compared with the blank control group at an amount of 1% and 5%.

TABLE-US-00007 TABLE 4 Water loss protection rate (%) Example 1 76 Example 2 75 Example 3 73 Example 4 70 Example 5 68 Example 6 66 Comparison Example 1 15 Comparison Example 2 16 Comparison Example 3 14 Comparison Example 4 18 Comparison Example 5 16 Comparison Example 6 20 Comparison Example 7 22

[0125] As can be seen from Table 4, compared with Comparison Examples 1-7, the water loss protection rate was greatly improved Examples 1-6 by adjusting the ratio of the five components of Astragalus membranacus, Radix sileris, Rhizoma gastrodiae, Calendula officinalis and Albizzia julibrissin; in addition, compared with Comparison Examples 4-6, the water loss protection rate was further improved in Examples 1-3 by further controlling the weight fractions of Astragalus membranacus, Radix sileris, Rhizoma gastrodiae, Calendula officinalis and Albizzia julibrissin.

[0126] (4) Security Data

[0127] The plant composition and extract preparations were tested for a series of safety tests to confirm from multiple angles that they are safe and non-irritating and applicable to the use of a wide range of people.

[0128] 1. Multiple (Acute) Skin Irritation Test

[0129] The multiple (acute) skin irritation test was carried out by reference to Safety and Technical Standards for Cosmetics (2015 edition), and the results showed that the Chinese medicine plant composition was safe and non-irritating at a concentration of 10% or less.

[0130] 2. Acute Ocular Irritation Test

[0131] The acute ocular irritation test was carried out by reference to Safety and Technical Standards for Cosmetics (2015 edition), and the results showed that the Chinese medicine plant composition was safe and non-irritating at a concentration of 10% or less.

[0132] 3. Skin Allergy Test

[0133] The skin allergy test was carried out by reference to Safety and Technical Standards for Cosmetics (2015 edition), and the results showed that the Chinese medicine plant composition had no allergenicity at a concentration of 10% or less.

[0134] 4. Phototoxicity Test

[0135] The 3T3 neutral red intake phototoxicity test was carried out by reference to GB/T 21769-2008 Chemical-In vitro 3T3 Neutral Red Uptake Phototoxicity Test Method, and the plant composition is expected to be non-phototoxic.

[0136] 5. Human Skin Patch Test

[0137] The human skin patch test was carried out by reference to Safety and Technical Standards for Cosmetics (2015 edition), and the results showed that the Chinese medicine plant composition had no adverse reaction to the human body at a concentration of 10% or less.

[0138] 6. Erythrocytes Hemolysis and Coagulation Test

[0139] The erythrocytes hemolysis and coagulation test was carried out by reference to Red Blood Cell Test System provided by European Centre for the Validation of Alternative Methods, ECVAM, and the results showed that the Chinese medicine plant composition had no adverse reaction to the human body at a concentration of 10% or less.

Example 7

[0140] A skin care product was prepared from the the traditional Chinese medicine plant composition prepared by Example 1, comprising in percentages by mass:

TABLE-US-00008 the adjuvant phase A: xanthan gum 0.06% glycerol 2% the adjuvant phase B: glyceryl stearate 2.3%.sup. cetearyl alcohol 1% pentaerythritol distearate 1.6%.sup. hydrogenated polyisobutylene 3% octoic acid 3% poly dimethyl silicon 2% isostearyl isostearate 3% the adjuvant phase C: methyl isothiazolinone 0.06% phenoxyethanol 0.4%; the traditional Chinese medicine plant composition 10%; prepared by Example 1 with the balance being water.

[0141] A preparation method of the skin care product, comprising the following steps:

[0142] (1) the xanthan gum in the adjuvant phase A was added to the glyceryl and evenly blended, and heated to 80-85 C.;

[0143] (2) the raw materials of the adjuvant phase B were mixed, and heated to 80-85 C.;

[0144] (3) all the adjuvant phase B of the step (2) was added to the adjuvant phase A of the step (1), homogenized at 3000 r/min for 5-10 min, heated to 80 C. and stirred for 20-40 min following by starting cooling at a cooling rate of 1-2 C./min by stirring at a stirring rate of 30-50 r/min;

[0145] (4) when the temperature was lowered to 45 C., the adjuvant phase C and the traditional Chinese medicine plant composition prepared by Example 1 were added, stirred evenly, and continued to cool to below 38 C. to obtain the skin care product.

Example 8

[0146] The skin care product comprises in percentages by mass:

TABLE-US-00009 the adjuvant phase A: xanthan gum 0.08% glycerol 3% the adjuvant phase B: PEG-100 stearate 3% cetearyl alcohol 1.5%.sup. pentaerythritol distearate 2% hydrogenated polyisobutylene 4% capric triglyceride 4% poly dimethyl silicon 3% isostearyl isostearate 4% the adjuvant phase C: iodopropynyl butylcarbamate 0.08% ethylhexylglycerin 0.8%; the traditional Chinese medicine plant composition 5%; prepared by Example 1 with the balance being water.

[0147] The preparation method is the same as that of Example 7.

Example 9

[0148]

TABLE-US-00010 the adjuvant phase A: xanthan gum 0.02% glycerol 1.5%.sup. the adjuvant phase B: glyceryl stearate 2% cetearyl alcohol 0.5%.sup. pentaerythritol distearate 1% hydrogenated polyisobutylene 2% capric triglyceride 2% poly dimethyl silicon 1% isostearyl isostearate 2% the adjuvant phase C: methyl isothiazolinone 0.02% ethylhexylglycerin 0.2%; the traditional Chinese medicine plant composition 0.5%; prepared by Example 1 with the balance being water.

[0149] The preparation method is the same as that of Example 7.

[0150] Anti-Allergy Skin-Improving Sensitive Skin State in Five Dimensions

[0151] When the skin is sensitive, it is often accompanied by itching, redness, tension, pricking and other unpleasant feelings. Thirty subjects aged 20-55 years old who reported themselves with skin sensitivity were randomly divided into two groups, 5% of the anti-Allergy cream and 5% of commercial competitor cream were applied onto their faces respectively. Use feedback from the subjects were collected before use and 4 weeks after use in the form of questionnaires to visually evaluate the anti-Allergy and improving functions of the traditional Chinese medicine plant compositions on sensitive skin in five dimensions of relieving itching, eliminating redness, eliminating swelling, eliminating tension and eliminating pricking.

[0152] The result is shown in FIG. 4, and the traditional Chinese medicine plant composition added in an amount of 5% can improve the sensitive state of the skin in five dimensions of relieving itching, eliminating redness, eliminating swelling, tension and pricking.

[0153] In summary, the traditional Chinese medicine plant composition of the present application achieves the function of anti-Allergy sensitive skin through three ways of anti-Allergy itching, eliminating redness and swelling, and allergy recovery by employing the dsynergistic effects of the five components including stragalus membranacus, Radix sileris, Rhizoma gastrodiae, Calendula officinalis and Albizzia julibrissin and according to the idea of integral, syndrome differentiation and comprehensiveness of traditional Chinese medicine.

[0154] The applicant declares that the present invention is described in detail by the above-described embodiments, but the present invention is not limited to the above detailed process equipment and process flows, that is, it does not mean that the present invention must be implemented in accordance with the detailed process equipment and process flows described above. It will be apparent to those skilled in the art that any modifications of the present invention, equivalent substitutions of the materials for the product of the present invention, and additions of auxiliary ingredients, selections of the specific means and the like, are all within the protection and disclosure scopes of the present invention.