EDIBLE OIL-IN-WATER EMULSION COMPRISING EGG YOLK COMPONENTS

Abstract

The invention relates to a method of producing an edible oil-in-water emulsion having a pH in the range of 3.0 to 5.0 and comprising 30-78 wt. % of oil, 65-20 wt. % water and 0.5-6 wt. % hen's egg yolk proteinaceous component by dry weight, said egg yolk proteinaceous component consisting of a combination of low-density lipoprotein (LDL), livetin, high-density lipoprotein (HDL), and phosvitin, and the method comprises the steps of: heating a liquid aqueous mixture comprising a first egg yolk plasma fraction to produce heat treated egg yolk plasma liquid; a. preparing a first pre-emulsion by combining oil, water, egg yolk granules fraction and optionally a second egg yolk plasma fraction; b. combining the heat treated egg yolk plasma liquid with the pre-emulsion to produce a second pre-emulsion; c. Adding one or more acidulants; and d. homogenizing the second pre-emulsion to obtain an oil-in-water emulsion;
wherein the egg yolk proteinaceous component of the oil-in-water emulsion has the following composition: 60-75 wt. % LDL; 8-14 wt. % livetin; 11-18 wt. % HDL; 2-5 wt. % phosvitin.

Claims

1. A method of preparing an edible oil-in-water emulsion having a pH in the range of 3.0 to 5.0 and comprising 30-78 wt. % of oil, 65-20 wt. % water and 0.5-6 wt. % hen's egg yolk proteinaceous component by dry weight, said egg yolk proteinaceous component consisting of a combination of low-density lipoprotein (LDL), livetin, high-density lipoprotein (HDL), and phosvitin, said method comprising the steps of: a) heating a liquid aqueous mixture comprising a first egg yolk plasma fraction at a temperature of 65 C. to 90 C. for 2 to 60 minutes to produce heat treated egg yolk plasma liquid comprising heat treated egg yolk plasma fraction; b) preparing a first pre-emulsion by combining oil, water, egg yolk granules fraction and optionally a second egg yolk plasma fraction; c) combining the heat treated egg yolk plasma liquid with the pre-emulsion to produce a second pre-emulsion; d) adding one or more acidulants; and e) homogenizing the second pre-emulsion to obtain an oil-in-water emulsion; wherein the egg yolk proteinaceous component of the oil-in-water emulsion has the following composition: 60-75 wt. % LDL; 8-14 wt. % livetin; 11-18 wt. % HDL; and 2-5 wt. % phosvitin.

2. The method according to claim 1, wherein the liquid aqueous mixture contains at least 40 wt. % water and wherein the egg yolk proteinaceous component of the liquid aqueous mixture has the following composition: 70-90 wt. % LDL 10-25 wt. % livetin; 0-10 wt. % HDL; and 0-5 wt. % phosvitin.

3. The method according to claim 1, wherein the first pre-emulsion contains 30-90 wt. % oil, 60-8 wt. % water and wherein the egg yolk proteinaceous component of the first pre-emulsion has the following composition: 15-80 wt. % HDL; 4-20 wt. % phosvitin. 7-65 wt. % LDL; and 0-12 wt. % livetin.

4. The method according to claim 1, wherein the first pre-emulsion is prepared by combining oil, water, egg yolk granules fraction and a second egg yolk plasma fraction and wherein the egg yolk proteinaceous component of the first pre-emulsion has the following composition: 15-70 wt. % HDL; 4-18 wt. % phosvitin. 17-65 wt. % LDL; and 1-12 wt. % livetin.

5. The method according to claim 4, wherein the second egg yolk plasma fraction and the granules egg yolk fraction are employed in the first pre-emulsion in a dry weight ratio in the range of 1:1 to 1:3.5.

6. The method according to claim 4, wherein the first egg yolk plasma fraction and the second egg yolk plasma fraction are employed in the method in a dry weight ratio in the range of 1:1 to 19:1.

7. The method according to claim 1, wherein the liquid aqueous mixture consists of the first egg yolk plasma fraction or is prepared by combining the first egg yolk plasma fraction with water, and wherein the first egg yolk plasma fraction and the second egg yolk plasma fraction contain 80-90% LDL and 10-20% livetin, both percentages being calculated by weight of the egg yolk proteinaceous component.

8. The method according to claim 1, wherein the egg yolk granules fraction contains 65-75% HDL, 13-20% phosvitin and 10-14% LDL, all percentages being calculated by weight of the proteinaceous component.

9. The method according to claim 1, wherein the method comprises adding one or more acidulants selected from acetic acid, citric acid, malic acid and lactic acid.

10. The method according to claim 9, wherein the one or more acidulants are added in a total concentration of 0.05-8% by weight of water.

11. The method according to claim 10, wherein the one or more acidulants are added to the second pre-emulsion before the homogenization.

12. The method according to claim 1, wherein the heated liquid aqueous mixture contains 0.2-10, sodium chloride in dissociated form.

13. The method according to claim 1, wherein the second pre-emulsion is homogenised in a colloid mill, a high-pressure homogenizer or an inline homogenizer.

14. An oil-in-water emulsion that is obtained by the method according to claim 1.

15. The method according to claim 5, wherein the second egg yolk plasma fraction and the granules egg yolk fraction are employed in the first pre-emulsion in a dry weight ratio in the range of 1:2 to 1:3.

16. The method according to claim 6, wherein the first egg yolk plasma fraction and the second egg yolk plasma fraction are employed in the method in a dry weight ratio in the range of 2:1 to 8:1.

17. The method according to claim 16, wherein the first egg yolk plasma fraction and the second egg yolk plasma fraction are employed in the method in a dry weight ratio in the range of 3:1 to 4:1.

18. The method according to claim 12, wherein the heated liquid aqueous mixture contains 0.4-5 wt. % sodium chloride in dissociated form.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0039] Thus, the present invention provides a method of preparing an edible oil-in-water emulsion having a pH in the range of 3.0 to 5.0 and comprising 30-78 wt. % of oil, 65-20 wt. % water and 0.5-6 wt. % egg yolk proteinaceous component by dry weight, said egg yolk proteinaceous component consisting of a combination of low-density lipoprotein (LDL), livetin, high-density lipoprotein (HDL), and phosvitin, said method comprising the steps of: [0040] a. heating a liquid aqueous mixture comprising a first egg yolk plasma fraction at a temperature of 65 C. to 90 C. for 2 to 60 minutes to produce heat treated egg yolk plasma liquid comprising heat treated egg yolk plasma fraction; [0041] b. preparing a first pre-emulsion by combining oil, water, egg yolk granules fraction and optionally a second egg yolk plasma fraction; [0042] c. combining the heat treated egg yolk plasma liquid with the pre-emulsion to produce a second pre-emulsion; and [0043] d. homogenizing the second pre-emulsion to obtain an oil-in-water emulsion; wherein the egg yolk proteinaceous component of the oil-in-water emulsion has the following composition: [0044] 60-75 wt. % LDL; [0045] 8-14 wt. % livetin; [0046] 11-18 wt. % HDL; [0047] 2-5 wt. % phosvitin.

[0048] The term egg yolk as used herein refers to the yolk obtained from bird eggs, most preferably hen's eggs.

[0049] The term high density lipoprotein (HDL) as used herein refers to a protein-lipid complex that is found in substantial concentrations in birds egg yolk. HDL comprises a protein with a hydrophobic pocket that holds the lipid component. HDL contains 75-80% apoproteins and 20-25% lipids. These lipids are composed of 65% phospholipids, 30% triglycerides and 5% cholesterol. Two sub-groups of HDL can be separated by ion chromatography: alpha- and beta-HDL. Alpha-HDL contains 6 time more sialic acid and 2 times more phosphorus than beta-HDL. Consequently, alpha-HDL is more acidic than beta-HDL. Except for these differences, both types of HDL have similar chemical compositions. HDL has a molecular weight of approximately 400 kDa, a diameter of about 7-20 nm and a density of approximately 1.12 g/ml. Unlike LDL, HDL does not have a spherical structure, but its pseudo-molecular structure resembles that of globular proteins. Phospholipids contribute to stabilise the structure of HDL in water.

[0050] The term low density lipoprotein (LDL) as used herein refers to a globular complex that is a major component of birds egg yolk, said globular complex having a diameter of 17-60 nm and a density of about 0.982 g/ml. LDL comprises an inner core largely consisting of triglycerides and cholesteryl esters and a surface layer that mainly consists of phospholipids, cholesterol and apoproteins. The apoproteins represent 11-17 wt. % of LDL, the lipid components 83-89 wt. %. These lipids are composed of about 74% neutral lipids, 26% phospholipids. LDL is composed of 2 sub-groups: LDL, (10.10.sup.6 Da) and LDL.sub.2 (3.10.sup.6 Da). LDL, represent 20% of total LDL and contains twice the amount of proteins as LDL.sub.2. Chemical compositions of both types of LDL are similar. Proteins of LDL are composed of 6 apoproteins. The major apoprotein (130 kDa) accounts for more than 70% of the apoproteins. The second apoprotein represents about 20% of the apoproteins and its molecular weight is 15 kDa. Their isoelectric point is ranged from 6.5 to 7.3.

[0051] The term edible as used herein means that the emulsion can be ingested and consumed in reasonable quantities without any toxic or other acute negative health effect. It will be understood therefore that the acidic emulsion of the present invention preferably does not contain any nonfood grade additives.

[0052] The term egg yolk proteinaceous component as used herein, unless indicated otherwise, refers to the combination of HDL, phosvitin, LDL and livetin. Thus, the composition of the egg yolk proteinaceous component is determined by the respective concentrations of these of these four proteinaceous components. By definition, the sum of the concentrations of these four components in egg yolk proteinaceous component adds up to 100%. It is noted that besides HDL, phosvitin, LDL and livetin, egg yolk contains a few minor proteins that are not encompassed by the term proteinaceous component as used herein.

[0053] The respective concentrations of HDL, phosvitin, LDL and livetin can be determined using the analytical method described by Burley et al. (ISOLATION AND COMPOSITION OF AVIAN EGG YOLK GRANULES AND THEIR CONSTITUENT -AND -LIPOVITELLINS, Canadian Journal of Biochemistry and Physiology, 1961, Vol. 39, No. 8: pp. 1295-1307).

[0054] The benefits of the present invention are particularly pronounced in emulsions comprising from 50-75 wt. % of oil and 20-48 wt. % of water. Oil is preferably present in an amount of less than 72 wt %, preferably less than 70 wt %. It can preferably be present in an amount of from 50 to 72 wt %, preferably combined with from 26 to 48 wt % water; preferably from 50 to 70 wt %, more preferably from 50 to less than 70 wt % oil, preferably combined with from 28 to 48 wt % water; even more preferably of from 65 to less than 70 wt % oil, preferably combined with from 28 to 33 wt % water. Even more, preferably, the emulsion contains from 65-72 wt. % of oil and from 26-33 wt. % water even more preferably from 65-70 wt. % of oil and from 28-33 wt. % water. The combination of oil and water preferably constitutes at least 80 wt. %, more preferably at least 90 wt. %, even more preferably at least 94% of the oil-in-water emulsion. A typical example of an emulsion that can be prepared by the present method is a reduced fat mayonnaise.

[0055] The oil that is used in the preparation of the oil-in-water emulsion can suitably contain a variety of lipid ingredients such as triglycerides, diglycerides, monoglycerides, phospholipids and free fatty acids. Preferably, triglycerides represent at least 80 wt. %, more preferably at least 90 wt. % and most preferably at least 95 wt. % of the oil.

[0056] Preferably, at 20 C., the oil contains less than 20 wt. %, more preferably less than 10 wt. % of solid fat. Most preferably, at the aforementioned temperature the oil contains no solid fat.

[0057] The solid fat content of a fat at a temperature of t C. (N.sub.t) can suitably be determined using ISO 8292-1 (2012)Determination of solid fat content by pulsed NMR.

[0058] Typically, the oil-in-water emulsion comprises a dispersed oil phase having a mean diameter (d3,2) in the range of 0.5-75 m. As explained herein before the present invention enables the preparation of a stable acidic emulsion without the need of decreasing the oil droplet size to very low values, e.g. below 1 m. At the same time it is preferred that the oil droplet size is small enough to prevent significant coalescence. Hence, according to a preferred embodiment, the emulsion comprises a dispersed oil phase having a mean diameter (d3,2) in the range of 1-50 m, more preferably in the range of 2-20 m. The measurement of the particle size distribution is suitably performed using a laser diffraction based instrument (MASTERSIZER 2000). Samples are prepared by diluting 1 ml of sample with 9 ml of a 1% sodium dodecyl sulphate solution (SDS) (1:10) in order to deflocculate the oil droplets. Before measuring, the sample is agitated for approximately 30 seconds and left out for 1 h. The measurements are performed directly after processing. The value of the Sauter mean diameter d3.2 is calculated as follows:

[00001]$d_{3.2}=\frac{.Math..Math.n_i.Math.d_i^3}{.Math..Math.n_i.Math.d_i^2}$

[0059] The oil-in-water emulsion that is prepared in the present method typically has a pH in the range of 3.2-4.7, especially within the range of 3.4-4.0

[0060] The present method preferably comprises adding one or more acidulants, preferably selected from acetic acid, citric acid, malic acid and lactic acid. More preferably, the method comprises addition of acetic acid, citric acid or a combination thereof. It is noted that acetic acid can be added in the form of, for instance, vinegar. Citric acid can be added in the form of, for example, lemon juice.

[0061] The one or more acidulants are typically added in the present method in a total concentration of 0.05-8%, more preferably 0.1-6% by weight of water.

[0062] According to a particularly preferred embodiment, the one or more acidulants are added to the second pre-emulsion before the homogenization.

[0063] The heated aqueous liquid typically contains 0.2 to 10 wt. %, more preferably 0.4 to 5 wt. % sodium chloride in dissociated form.

[0064] The sodium chloride content of the oil-in-water emulsion is preferably in the range of 3-12%, more preferably of 4-10% and most preferably 4.5-9% by weight of water.

[0065] The acidic emulsion of the present invention, besides oil, water, egg yolk components, acidulants and salt, may suitably contain a variety of other ingredients, such as flavouring materials, colourings and anti-oxidants. Examples of flavouring materials that may advantageously be incorporated in the present emulsion include sucrose, mustard, herbs, spices, lemon juice and mixtures thereof. According to a particularly preferred embodiment, the emulsion contains at least one of sucrose and mustard.

[0066] The egg yolk that is employed in the present method preferably is hen's egg yolk.

[0067] The liquid aqueous mixture that is employed in step a) of the present method typically contains 40-80 wt. % water, preferably 45-70 wt. % water, more preferably 50-65 wt. % water.

[0068] The egg yolk proteinaceous component, i.e. the combination of LDL, livetin, HDL and phosvitin, typically constitutes 20-60 wt. %, more preferably 30-55 wt. % and most preferably 35-50 wt. % of the liquid aqueous mixture that is heated in the present method.

[0069] In accordance with a preferred embodiment, the liquid aqueous mixture is heated at a temperature of 65 C. to 85 C. for 1 to 45 minutes, more preferably at a temperature of 68 C. to 77 C. for 2 to 30 minutes.

[0070] The liquid aqueous mixture employed in step a) of the present method preferably consists of the first egg yolk plasma fraction or is prepared by combining the first egg yolk plasma fraction with water. The first egg yolk plasma fraction preferably contains 80-90% LDL and 10-20% livetin, both percentages being calculated by weight of the egg yolk proteinaceous component.

[0071] The first pre-emulsion that is prepared in step b) of the present method preferably contains 30-90 wt. % oil, 60-8 wt. % water. More preferably, the first pre-emulsion contains 50-85 wt. % oil and 40-13 wt. % water. Even more preferably the first pre-emulsion contains 65-82 wt. % oil, 25-16 wt. % water. The combination of oil and water preferably constitutes at least 80 wt. %, preferably 90 wt. %, more preferably at least 95 wt. % of the first pre-emulsion.

[0072] The egg yolk proteinaceous component of the first pre-emulsion preferably has the following composition: [0073] 15-80 wt. % HDL; [0074] 4-20 wt. % phosvitin. [0075] 7-65 wt. % LDL; and [0076] 0-12 wt. % livetin.

[0077] The proteinaceous component of the first pre-emulsion comprises egg yolk components for egg yolk granules fraction and optionally also egg yolk components from egg yolk plasma fraction.

[0078] According to a particularly preferred embodiment, the first pre-emulsion is prepared by combining oil, water, egg yolk granules fraction and an egg yolk plasma fraction. The latter egg yolk plasma fraction is referred to herein as the second egg yolk plasma fraction to distinguish said plasma fraction from the first egg yolk plasma fraction that is employed in step a) of the present method. The second egg yolk plasma fraction preferably contains 80-90% LDL and 10-20% livetin, both percentages being calculated by weight of the egg yolk proteinaceous component.

[0079] The first pre-emulsion can be produced in any equipment that is capable of thoroughly mixing the ingredients contained therein, e.g. a Silverson mixer.

[0080] The egg yolk granules fraction that is employed in the present method preferably contains 65-75% HDL, 13-20% phosvitin and 10-14% LDL, all percentages being calculated by weight of, the proteinaceous component.

[0081] In a preferred embodiment of the present method the second egg yolk plasma fraction and the granules egg yolk fraction are employed in the first pre-emulsion in a dry weight ratio in the range of 1:1 to 1:3.5, preferably in the range of 1:2 to 1:3.

[0082] In case the preparation of the first pre-emulsion comprises the use of a second egg yolk plasma fraction said first pre-emulsion preferably has the following composition:

15-70 wt. % HDL;

[0083] 4-18 wt. % phosvitin.

17-65 wt. % LDL; and

[0084] 1-12 wt. % livetin.

[0085] Even more preferably, the first pre-emulsion has the following composition:

15-50 wt. % HDL;

[0086] 4-12 wt. % phosvitin.

20-65 wt. % LDL; and

[0087] 5-12 wt. % livetin.

[0088] The first egg yolk plasma fraction and the second egg yolk plasma fraction are preferably employed in the present method in a dry weight ratio in the range of 1:1 to 19:1, preferably in the range of 2:1 to 8:1, more preferably in the range of 3:1 to 4:1.

[0089] The second pre-emulsion is preferably homogenised in a colloid mill (e.g. ex Ross), a high pressure homogeniser or an inline homogeniser (e.g. ex. Maelstrom IPM).

[0090] The present homogenized emulsion may suitably be subjected to a heat treatment to increase the shelf life.

[0091] Another aspect of the invention relates to an oil-in-water emulsion that is obtained by the method as described herein.

[0092] The invention is further illustrated by the following non-limiting examples.

EXAMPLES

[0093] Measuring the Storage Modulus G

[0094] The storage modulus G is indicative of an object's ability to be deformed elastically (i.e., non-permanently) when a force is applied to it. The term storage in storage modulus refers to the storage of the energy applied to the sample. The stored energy is recovered upon the release of the stress.

[0095] The storage modulus of an oil-in-water emulsion is suitably determined by a dynamic oscillatory measurement in which the shear stress is varied (from low to high stress) in a sinusoidal manner. The resulting strain and the phase shift between the stress and strain is measured. From the amplitude of the stress and the strain and the phase angle (phase shift) the storage modulus is calculated. Herein, the G (Pa) is taken at the plateau region at low stress (linear viscoelastic region).

[0096] The storage modulus G of the emulsions described in the following examples was measured as follows: [0097] The sample is rested for 3 minutes after the introduction into the rheometer (e.g. TA AR2000EX) to allow relaxation of the stresses accumulated due to the loading of the sample. [0098] Using a 4 cm 2 cone, a stress sweep is applied in which the oscillatory stress is increased from 0.1 to 1768 Pa in logarithmic steps (15 per decade). This step is terminated when the phase angle exceeds 80.

Example 1

[0099] Egg yolk plasma fraction and egg yolk granules fractions were prepared from commercially available egg yolk (brand name: Eggstra, supplier: Van Tol Convenience Food) by the following procedure: [0100] dilute the egg yolk with an equal amount of an aqueous 0.17M NaCl solution and mildly stir for about 1 hour [0101] centrifuge the egg yolk dispersion at 10,000 g for 45 minutes at 4 C. [0102] remove supernatant by decanting [0103] centrifuge the supernatant at 10,000 g for 45 minutes at 4 C. [0104] the supernatant obtained after the last mentioned centrifugation step represents the egg yolk plasma fraction [0105] the combined sediments obtained during the centrifugation steps are dispersed in an aqueous 0.17M NaCl solution to produce a stock dispersion having a dry matter content of approximately 40 wt. %. This dispersion represents the egg yolk granules fraction

[0106] A part of the egg yolk plasma fraction so obtained was heated in a water bath to a temperature of 70-71 C. for 5 minutes. Next, the heated plasma fraction was rapidly chilled using an ice bath and water was added to compensate for water loss as a result of evaporation.

[0107] The unheated egg yolk plasma fraction, the heated egg yolk plasma fraction and the egg yolk granules fraction were used to prepare mayonnaise products (products 1 and 2) having an oil content of 70 wt. % on the basis of the recipes shown in Table 1. As a comparison, a mayonnaise of identical composition (Reference product) was prepared using the whole egg yolk. The recipe of this reference product is also shown in Table 1.

TABLE-US-00001 TABLE 1 Ref. 1 2 Whole egg yolk (dry matter) 3.50 Egg yolk plasma fraction (dry matter) 1.35 0.68 Heated egg yolk plasma fraction (dry matter) 1.35 2.02 Egg yolk granules fraction (dry matter) 0.80 0.80 Salt 2.25 2.25 2.25 Sucrose 1.30 1.30 1.30 Preservatives 0.03 0.03 0.03 Oil 70.00 70.00 70.00 Water and vinegar 22.92 22.92 22.92 Total 100.00 100.00 100.00

[0108] The reference product was prepared by mixing the egg yolk with the other ingredients, except for the oil and vinegar. Next, the oil was slowly added at room temperature using a Silverson L4RT-A homogenizer at 5000 rpm over approximately 8 minutes. Subsequently, vinegar was added and the emulsion was mixed for 2 additional minutes using the homogenizer at 5000 rpm. Then, the emulsion was passed once through a colloid mill (IKA magicLAB, MK) with a grinding gap size of 0.398 mm operating at 7600 rpm. The emulsion samples so produced were stored in glass jars at 5 C.

[0109] Mayonnaise products 1 and 2 were prepared by mixing the egg yolk granules fraction and the unheated egg yolk plasma fraction with the other ingredients, except for the oil and vinegar. Next, the oil was slowly added at room temperature using a Silverson L4RT-A homogenizer at 5000 rpm over approximately 8 minutes. Next, the heated egg yolk plasma fraction was added followed by homogenization for another 1-2 minutes at 5000 rpm. Subsequently, vinegar was added and the emulsions were further processed in the same way as the Reference product.

[0110] The pH and the shear storage modulus G (at 20 C.) of the mayonnaise products were measured. The results are shown in Table 2.

TABLE-US-00002 TABLE 2 Ref. 1 2 pH 3.8 3.8 3.8 G (Pa) 487 546 623

Example 2

[0111] The unheated egg yolk plasma fraction, the heated egg yolk plasma fraction and the egg yolk granules fraction of Example 1 were used to prepare a mayonnaise product (product 1) having an oil content of 70 wt. % on the basis of the recipe shown in Table 3. A reference mayonnaise (Reference product) of identical composition was prepared using whole egg yolk. The recipe of the reference product is also shown in Table 3.

TABLE-US-00003 TABLE 3 Wt. % Ref. 1 Whole egg yolk (dry matter) 3.50 Egg yolk plasma fraction (dry matter) 0.68 Heated egg yolk plasma fraction (dry matter) 2.02 Egg yolk granules fraction (dry matter) 0.80 Salt 1.78 1.78 Sucrose 1.30 1.30 Preservatives 0.10 0.10 Oil and flavouring additives 70.19 70.19 Water and vinegar 23.13 23.13 Total 100.00 100.00

[0112] The mayonnaise products were prepared at reduced pressure (about 0.5 bars) using an ESCO-LABOR mixer with a double jacketed stainless steel 10 L vessel equipped with a scraper operating at about 140 rpm.

[0113] Mayonnaise product 1 was prepared by mixing the egg yolk with the other ingredients, except for the oil and vinegar, followed by homogenization at 800 rpm for 3 minutes. Next, oil was added via a funnel at a rate of 0.5 L/min with the homogenizer operating at 4500 rpm, followed by 5 more minutes of homogenization. Subsequently, vinegar was added and the emulsion was mixed for 2 more minutes using the homogenizer at 5000 rpm. Then, the emulsion was passed once through a colloid mill (IKA LABOR-PILOT 2000/4, MK) with a grinding gap size of 0.398 mm operating at 7600 rpm. The emulsion samples so produced were stored in glass jars at 5 C.

[0114] Mayonnaise product 1 was prepared in the same way as the Reference product, except that in the first step of the process unheated egg yolk plasma fraction and egg yolk granules fraction were used instead of egg yolk. In addition, heated egg yolk plasma fraction was added after the addition of the oil and before the addition of the vinegar. The heated egg yolk plasma fraction was added via a funnel, followed by homogenization at 4500 rpm for 4 minutes.

[0115] The shear storage modulus G (at 20 C.) of the mayonnaise products was measured. The results are shown in Table 4.

TABLE-US-00004 TABLE 4 Ref. 1 G (Pa) 577 771

Example 3

[0116] The unheated egg yolk plasma fraction, the heated egg yolk plasma fraction and the egg yolk granules fraction of Example 1 were used to prepare mayonnaise products (products 1 and 2) having an oil content of 70 wt. % on the basis of the recipe shown in Table 3. Reference mayonnaises (Reference products 1 and 2) of identical composition were prepared using whole egg yolk. The recipes of these reference products are also shown in Table 5.

TABLE-US-00005 TABLE 5 Ref. 1 1 Ref. 2 2 Whole egg yolk (dry matter) 3.00 4.0 Egg yolk plasma fraction 0.58 0.77 (dry matter) Heated egg yolk plasma fraction 1.73 2.31 (dry matter) Egg yolk granules fraction 0.69 0.92 (dry matter) Salt 1.78 1.78 2.25 2.25 Sucrose 1.30 1.30 1.30 1.30 Preservatives 0.10 0.10 0.03 0.03 Oil 70.00 70.00 70.00 70.00 Water and vinegar 23.82 23.82 22.92 22.92 Total 100.00 100.00 100.00 100.00

[0117] The mayonnaise products were prepared in the same way as described in Example 1.

[0118] The pH and the shear storage modulus G (at 20 C.) of the mayonnaise products were measured. The results are shown in Table 6.

TABLE-US-00006 TABLE 6 Ref. 1 1 Ref. 2 2 pH 3.8 3.8 3.8 3.8 G (Pa) 243 409 632 783

Example 4

[0119] The unheated egg yolk plasma fraction, the heated egg yolk plasma fraction and the egg yolk granules fraction of Example 1 were used to prepare a mayonnaise product (product 1) having an oil content of 65 wt. % on the basis of the recipe shown in Table 7. A reference mayonnaise (Reference product) of identical composition was prepared using whole egg yolk. The recipe of the reference product is also shown in Table 7.

TABLE-US-00007 TABLE 7 Wt. % Ref. 1 Whole egg yolk (dry matter) 3.50 Egg yolk plasma fraction (dry matter) 0.68 Heated egg yolk plasma fraction (dry matter) 2.02 Egg yolk granules fraction (dry matter) 0.80 Salt 2.25 2.25 Sucrose 1.30 1.30 Preservatives 0.03 0.03 Oil 65.00 65.00 Water and vinegar 27.92 27.92 Total 100.00 100.00

[0120] The mayonnaise products were prepared in the same way as described in Example 1.

[0121] The shear storage modulus G (at 20 C.) of the mayonnaise products was measured. The results are shown in Table 8.

TABLE-US-00008 TABLE 8 Ref. 1 G' (Pa) 245 405

Example 5

[0122] The unheated egg yolk plasma fraction, the heated egg yolk plasma fraction and the egg yolk granules fraction of Example 1 were used to prepare a mayonnaise product (product 1) having an oil content of 50 wt. % on the basis of the recipe shown in Table 9. A reference mayonnaise (Reference product) of identical composition was prepared using whole egg yolk. The recipe of the reference product is also shown in Table 9.

TABLE-US-00009 TABLE 9 Wt. % Ref. 1 Whole egg yolk (dry matter) 3.75 Egg yolk plasma fraction (dry matter) 0.72 Heated egg yolk plasma fraction (dry matter) 2.17 Egg yolk granules fraction (dry matter) 0.86 Salt 2.25 2.25 Sucrose 2.00 2.00 Preservatives 0.01 0.01 Oil and flavouring additives 50.24 50.24 Water and vinegar 41.75 41.75 Total 100.00 100.00

[0123] The mayonnaise products were prepared in the same way as described in Example 1, except that for all pre-emulsification steps the Silverson L4RT-A homogenizer was used at a shear rate of 7000 rpm. In addition, the final product was obtained via homogenization in a high pressure homogenizer at 600 bar, instead of a colloid mill.

[0124] The shear storage modulus G (at 20 C.) of the mayonnaise products was measured. The results are shown in Table 10.

TABLE-US-00010 TABLE 10 Ref. 1 G (Pa) 566 851