Material comprising outer layer having entanglement of hydrophobic polymer host blended with anhydride functionalized hydrophobic polymer co-host and hydrophilic guest

Abstract

The present disclosure provides, inter alia, a method of producing an outer layer material for forming into a structure and that comprises an entanglement having a hydrophobic polymer host and a hydrophilic guest, including in one embodiment the steps of: intermingling cloaked hydrophilic guest complexes with the hydrophobic host; crosslinking molecules of the guest with the guest; and performing a hydrolysis reaction.

Claims

1. A material comprising: (i) an outer layer comprising an entanglement; and (ii) a hydrophilic outer surface of said outer layer, wherein said entanglement comprises a physical blend of a hydrophobic polymer host and an anhydride functionalized hydrophobic polymer co-host, and a hydrophilic guest; wherein said hydrophobic polymer host comprises a polyolefin; wherein said anhydride functionalized hydrophobic polymer co-host comprises a polyolefin which has been functionalized with anhydride functional groups; and wherein said hydrophilic guest comprises a salt of a glycosaminoglycan; said entanglement having been synthesized by: (a) intermingling cloaked hydrophilic guest complexes with said physical blend of said host and said co-host; (b) crosslinking molecules of said guest with said guest; and (c) performing a hydrolysis reaction.

2. The material according to claim 1, wherein: said co-host is selected from the group consisting of: maleic anhydride-graft-polyethylene, maleic anhydride-graft-polypropylene; and maleic anhydride-graft-polystyrene.

3. The material according to claim 1, wherein said cloaked hydrophilic guest complexes are selected from the group consisting of silylated guest complexes and acylated guest complexes.

4. The material according to claim 3, wherein said cloaked hydrophilic guest complexes are silylated guest complexes.

5. The material according to claim 1, wherein said entanglement is surface treated with glycosaminoglycan.

6. The material according to claim 3, wherein: said co-host is selected from the group consisting of: maleic anhydride-graft-polyethylene, maleic anhydride-graft-polypropylene; and maleic anhydride-graft-polystyrene.

7. The material according to claim 4, wherein said entanglement is surface treated with glycosaminoglycan.

8. The material according to claim 2, wherein: said functionalized polyolefin comprises a polyolefin backbone to which the anhydride functional groups have been grafted; and said entanglement, after performing said hydrolysis reaction, having also been: (a) doped with a host-material crosslinking agent and an antioxidant; (b) surface treated using glycosaminoglycan; and (c) crosslinked, using poly(diisocyanate), to crosslink at least a portion of said guest compound with itself.

9. The material according to claim 8, molded while at least a portion of said host and said co-host are being crosslinked using said host-material crosslinking agent.

10. The material according to claim 9, wherein said host-material crosslinking agent is selected from the group consisting of silanes and peroxides.

11. The material according to claim 1, wherein said hydrophilic outer surface of said outer layer is selected from the group consisting of: a bearing surface adapted for mechanical wear; a flexible barrier surface separating a first and second area; a transparent member surface; an in vivo implant surface; a drag reduction surface; a reaction resin surface; a topical dressing surface; and a dental splint surface.

12. The material according to claim 1, wherein said polyolefin which has been functionalized with anhydride functional groups comprises a polyolefin backbone into which the anhydride functional groups have been incorporated.

13. The material according to claim 12, wherein said hydrophilic outer surface of said outer layer is selected from the group consisting of: a bearing surface adapted for mechanical wear; a flexible barrier surface separating a first and second area; a transparent member surface; an in vivo implant surface; a drag reduction surface; a reaction resin surface; a topical dressing surface; and a dental splint surface.

14. A medical device comprising the material according to claim 1.

Description

BRIEF DESCRIPTION OF THE DRAWINGS AND ATTACHMENT A

(1) For purposes of illustrating the innovative nature plus the flexibility of design and versatility of the preferred outer layer material, system and method disclosed hereby, the invention will be better appreciated by reviewing the accompanying drawings (in which like numerals, if included, designate like parts), and to an extent, ATTACHMENT A to co-pending parent utility application Ser. No. 10/283,760 filed 29 Oct. 2002, to which priority is claimed hereby. One can appreciate the many features that distinguish the instant invention from known structures and fabrication techniques. The drawings have been included to communicate the features of the innovative outer layer, system and associated method of producing according to the invention by way of example, only, and are in no way intended to unduly limit the disclosure hereof.

(2) FIG. 1 is a partial, enlarged, cross-sectional view of a system of the invention (at 10) having a base 18 and outer layer 17.

(3) FIG. 2 is a partial, enlarged, cross-sectional view of a system (at 20) having a base 28 and outer layer 24 produced according to the invention.

(4) FIGS. 3A-3D are depictions of various molecular structures shown here, once again by way of example, as representative structures thereof.

(5) FIG. 4 is a flow diagram depicting details of a method 40 of producing an outer layer material having an entanglement according to the inventionillustrated are core, as well as further distinguishing, features of the invention employing features such as those represented and depicted in FIGS. 1-2 and 3A-3D.

(6) FIGS. 5 and 5A diagrammatically depict embodiments of the method, including chemical structures associated therewith; illustrated are core, as well as further distinguishing/additionally unique, features. Notation has been made in a few steps of FIGS. 5, 5A to steps labeled in FIGS. 4 and 7A-7B, for purposes of cross-reference only.

(7) FIG. 6 diagrammatically illustrates an alternative method 70, highlighting use of a host blend composed of a hydrophobic polymer host and an anhydride functionalized hydrophobic polymer co-host, according to the invention.

(8) FIGS. 7A and 7B, taken together, depict steps of an alternative method 140 of producing an outer layer material having an entanglement according to the invention. Illustrated are core, as well as further distinguishing/additionally unique, features.

(9) ATTACHMENT A to co-pending utility application Ser. No. 10/283,760 filed 29 Oct. 2002, to which priority is claimed hereby, a twenty-eight page manuscript authored by the applicants entitled IPN Surface Modification of Ultra High Molecular Weight Polyethylene for Lowering Friction and Wear in Total Joint Replacement was included with co-pending utility application Ser. No. 10/283,760 for its technical background, analysis and support of the system, outer layer, and method of the invention, is hereby incorporated herein by reference to the extent necessary to aid in further understanding the mathematical and rigorous engineering analyses performed by the applicants.

DETAILED DESCRIPTION OF THE DRAWINGS

(10) The partial, enlarged, cross-sectional view of a system 10 in FIG. 1 depicts, in a schematic fashion, a base 18 and an outer layer 17. While the outer surface 12 is depicted as planar it is by no means limited thereto; and rather, the hydrophilic surface 12 is likely, depending upon application, to have some curvature or slope as depicted and labeled 22 in the enlarged sectional view of system 20 in FIG. 2. The outer layer 17 (or 24 in FIG. 2) has an entanglement identified above and throughout, of a hydrophobic host and a hydrophilic guest material. Referring, also, to the method depicted in FIG. 4 (box 42), the entanglement preferably comprises an intermingling of cloaked hydrophilic guest and a hydrophobic polymer host, or hydrophobic polymer host blend of host and co-host, wherein molecules of the guest have been crosslinked with each other. The hydrophilic guest of the entanglement may comprise a compound selected from a specified group consisting of polyions, polysaccharides (including the glycosaminoglycan, hyaluronic acid), salts of glycosaminoglycans, nucleic acids, polyvinylpyrrolidones, peptides, polypeptides, proteins, lipoproteins, polyamides, polyamines, polyhydroxy polymers, polycarboxy polymers, phosphorylated derivatives of carbohydrates, sulfonated derivatives of carbohydrates, interleukin-2, interferon, and phosphorothioate oligomers, with-or-without amino acids, as well as other hydrophilic polymers. As further identified: polyhydroxy polymers include, among others, polyvinyl alcohol and polyethylene glycol; and polycarboxy polymers include, among others, carboxymethylcellulose, alginic acid, sodium alginate, and calcium alginate.

(11) The intermingling of the guest (box 50, FIG. 4) and host, or host blend, includes a physical tangling, whether it also comprises crosslinking by primary bonding (chemical/covalent bonding) there-between. Depending upon the type of intermingling employed, the guest selected for intermingling with the host, the type of crosslinking of the guest (by chemical crosslinking or by way of thermal, ultra-violet UV, or other suitable energy source), as well as composition and form of the host (e.g., fully- or partially-consolidated porous structure, in powdered form, in solution, and so on), cloaking (box 46 in FIG. 4) may be by any way suitable, such as by way of performing silylation or acylation, of the guestmaking it more hydrophobic. Under certain circumstances, using complexes of the guest may be desirable or even necessary (box 44, FIG. 4)especially where the guest is not sufficiently hydrophobic to undergo the selected cloaking technique. The cloaked groups are then returned to an initial hydrophilic state (box 54, FIG. 4) to produce a generally hydrophilic outer surface of the layer, this can be by performing a hydrolysis reaction.

(12) As depicted in FIGS. 1 and 2, a base (18 or 28) with which an outer layer of the invention is inter-linked by suitable means (depending upon final application and the environment in which the system will be placed for operation), is likely comprised of host material. At 16 in FIG. 1, a transition section of the outer layer which, as represented, may include a slightly different consistency or density of intermingled host and guest, or might include an adhesive or film or other means used for inter-connecting/inter-linking the outer layer with a base comprising the host material.

(13) For purposes of a more-thorough understanding of the invention and the representative examples discussed throughout, FIGS. 3A-3D depict various molecular structures employed in connection with producing an outer layer according to the invention. As shown: the chemical formula for (plain) hyaluronic acid (HA) at 30 in FIG. 3A; in FIG. 3B the formula for cetyltrimethylammonium bromide 32, as is the structure of the salt cetylpyridinium chloride monohydrate 34; in FIG. 3C the guest complex represented by the expression HA.sup.-QN.sup.+, where HA represents hyaluronic acid and QN.sup.+ represents a cation; and in FIG. 3D a silylated HA.sup.-QN.sup.+ complex is depicted. As one can appreciate, the cloaking of HA.sup.-QN.sup.+ complexes results in the hydrophilic groups being replace with silylated functional groups: hydrogen (H) has been replace with Si(CH.sub.3).sub.3.

(14) As used herein, for reference, the following acronyms are identified as listed:

(15) BSAN, OBis(trimethylsilyl)acetamide, a silylation agent.

(16) DMFN, N-Dimethyl formamide

(17) DMSODimethyl sulphoxide

(18) HAHyaluronic acid

(19) HA-CPCthe complex of HA polyanion and cetylpyridinium salt.

(20) HA.sup.-QN.sup.+the complex of HA polyanion and long-chain paraffin ammonium cation.

(21) HA-CTAB or HA-CTAspecific examples of an HA.sup.-QN.sup.+ complexis the complex of HA polyanion and cetyltrimethylammonium salt (CTAB) or CTA.sup.+ in ammonium salt cation, the form it takes in the HA.sup.-QN.sup.+ complex.

(22) HMDShexamethyldisilazane, a silylation agent.

(23) THFTetrahydrofuran

(24) TMCStrimethylchlorosilane, a silylation agent.

(25) QN.sup.+long-chain paraffin ammonium cation; which is also known as a long-chain aliphatic quaternary ammonium salt.

(26) The flow diagram in FIG. 4 details a method 40 of producing an outer layer material having an entanglement according to the inventionillustrated are core, as well as further distinguishing, features of the invention employing features such as those represented and depicted in FIGS. 1-2 and 3A-3D. Steps of the method include, as shown: Temporarily cloaking (silylation, acylation, etc.) at least a portion of the hydrophilic groups of the guest (46); intermingling at least a portion of the cloaked guest with the porous polymeric structure by diffusing the cloaked guest into at least a portion of the structure's pores (50); within the pores, crosslinking at least a portion of the molecules of the guest with the guest (52); and then removing the cloaking (54) to produce a generally hydrophilic outer surface of the outer layer. Alternatively, entangling (50, 52) of the guest and the host can be by (a) producing a mixture comprising the cloaked guest and the host, and (b) crosslinking at least a portion of the molecules of the guest with the guest. This mixture can then be molded into the outer layer (58, 59a/59b). In the event the intermingling is done with the cloaked guest in powdered form (by, for example, vacuuming drying washed acylated guest 48) and the host in powdered form, once the cloaked groups have been returned to an initial hydrophilic state (54), once again, the outer layer may be produced by way of a thermal molding of the powdered form of the guest with the host (reference 58, 59a).

(27) FIGS. 5 and 5A diagrammatically depict embodiments of the method, including chemical structures associated therewith, namely, Silyl HA-CTA (step 46 directed to temporary cloakingsuch as by way of silylation, acylation, etc.of guest complexes, e.g., HA-CTA, into Silyl HA-CTA) and HA and HA-CTA (both associated with the step to produce complexes of hydrophilic guest, 44). For purposes of handy cross-reference only, notation has been made in a few boxes/steps of FIGS. 5, 5A to steps labeled in FIGS. 4 and 7A-7B. The end-result outer layer material is represented by (in-phantom box) 68 which uses the term micro-composite, and is identified as UHMWPE-HA, by way of example, here, since a UHMWPE porous structure 61 was used as host. As shown, the micro-composite (outer layer material) includes an entanglement comprising the intermingling of the cloaked hydrophilic guest and 61 either (a) a hydrophobic polymer host, or (b) a physical blend of a hydrophobic polymer host along with an anhydride functionalized hydrophobic polymer co-host, i.e., any polyolefin which has been functionalizedgrafted onto, or otherwise incorporated into the polymer backbone with anhydride functional groups (e.g., maleic anhydride-graft-polyethylene, more-commonly maleated polyethylene; maleic anhydride-graft-polystyrene, more-commonly maleated polystyrene; and so on). For example, if a UHMWPE porous structure is employed as host, box 61 (a), a maleic anhydride graft polyethylene porous structure can be employed as co-host, box 61 (b). The host and Silyl HA-CTA, box 65, in xylene solutionor a physical blend of host and co-host and Silyl HA-CTA, box 65may be intermingled 60, by way of soaking (as detailed further, elsewhere, herein).

(28) Once the guest and host blend of host and co-host are intermingled 60, a crosslinking of guest to itself (for example, treated via chemical crosslinking using a poly(diisocyanate) at 62) is done prior to recovering the hydrophilic groups (e.g., by hydrolysis 64) on the guest. Once the guest is returned to a pre-complex, native state (by removing the cloaking from the guest complexes which, as mentioned immediately above, can be done by hydrolysis, see FIG. 4 at 54, FIGS. 5, 5A at 64, and FIG. 7B at 154) the outer layer material can then be surface treated in double-dip fashion (e.g., FIGS. 5, 5A at 66; FIG. 7B at 157a) to result in a surface coating of the guest that is crosslinked to itself and the other guest molecules incorporated in earlier steps (FIGS. 5, 5A at 60, 62).

(29) Next shown, is another optional step 67 that includes doping with a host material crosslinking agent (such as silanes or peroxides) along with an antioxidant, which is followed by a molding step 69, 69Apreferably performed at an elevated temperatureduring which the crosslinking agent employed for the optional doping step 67, can also be employed to crosslink the host to itself, along with crosslinking co-host to host and co-host to itself (compare FIGS. 5, 5A at 69, 69A; FIG. 6 at 92; and FIG. 7B at 157b, 158). Suitable host material crosslinking agents contemplated for use during (optional) doping 67, and optionally, again, during molding 69A, include silanes or peroxides. An antioxidant (e.g., vitamin E) can be doped-in, along with the host crosslinking agent via treatment with a solution containing the host crosslinking agent and the antioxidant, with subsequent evaporation of the solvent (e.g. acetone). The antioxidant aids in preventing oxidation during the final molding step and any post-molding ageing due to residual free radicals left from the crosslinking agent. Preferably, the amounts of host crosslinking agent and antioxidant are adjusted to achieve a balance between obtaining a desirable level of host crosslinking and prevention of oxidation during processing and post-processing.

(30) Turning next to FIGS. 6 and 7A-7B, which diagrammatically highlight the use of a hydrophobic host blend comprising a hydrophobic polymer host 73a, 143a (e.g., a polyolefin such as polyethylene or polystyrene) and an anhydride functionalized hydrophobic polymer co-host 73b, 143b (e.g., a polyolefin functionalized with anhydride functional groups) to be intermingled with at least a portion of a modified hydrophilic guestcomplexed glycosaminoglycan such as HA.sup.-QN.sup.+, or HA complexed with ammonium salt, HA-CTA (box 74, 144)to which silyation or acylation is performed (to silylate the ammonia salt derivative, in effect, carrying out a temporary cloaking of the guest complexes) 146. Likewise, similar to the steps contemplated in FIG. 4 respectively at 48, 50, 52, 54 are steps 148, 150, 152, 154 of the FIGS. 7A-7B flow diagram. The steps labeled 158, 159a, 159b of FIG. 7B relating to molding the layer material as is or incorporating with a base, correspond with steps 58, 59a, 59b of FIG. 4. Even further unique are steps labeled 157a, 157b: 157a represents the double dip type treatment (compare with step 66, FIG. 6) such as a first dip to surface treat the material with a guest (such as the glycosaminoglycan, or GAG, HA), with a second dip being into a crosslinker such as poly(diisocyanate) to crosslink the GAG; and 157b represents the step to crosslink the host to itself, host to co-host, and co-host to itself using, e.g., silanes or peroxides (compare with step 69A, FIG. 5A and step 92, FIG. 6). Note also, step 82 of FIG. 6 can be mapped with step 62, FIGS. 5, 5A.

(31) An IPN is an intimate combination of at least two polymers; it is a material network where at least two polymer components are physically associated by being covalently linked. In general, in an IPN, at least one component is synthesized or cross-linked in the presence of the other, although the two components may or may not be bound together. It is intended that semi-IPNs fall within the category of IPNs.

(32) Polymeric materials are used in numerous biomedical applications. Polymers characteristically have smooth resilient surfaces. Ultra high molecular weight polyethylene (UHMWPE) is a biologically inert polymeric material that has long been used in total joint replacement (arthroplasty). Despite its many positive attributes such as biologically compatible and durable, the repetitive motion between mating surfaces as in a joint leads to the formation of UHMWPE wear debris. The presence of the wear debris is a negative outcome in all types of joints especially an arthroplasty where it can lead to joint loosening and failure of the total joint. The tradeoff to using UHMWPE for its superior mechanical strength and desirable modulus for bearing weight and repetitive use, is that it is extremely difficult, and some have considered impossible according to conventional practices, to produce an IPN using a very hydrophobic polymer such as UHMWPE and a very hydrophilic polymer such as HA. Though considerable effort has been spent by others to improve the wear resistance of UHMWPE in biomedical applications, including topical treatment to decrease the hydrophobic nature of the UHMWPE surface (such as dip-coating or grafting), these efforts have produced UHMWPE medical device/implants in need of greater surface lubricity (especially near joint areas) along with sufficient structural integrity for extended wear in repetitive use applications. Applicants' approach is the synthesis of a new IPN to exploit desirable characteristics of UHMWPE and a hydrophilic polymer, leading to a system/device having increased lubricity and reduced friction and wear.

(33) The step of diffusing according to the invention can include suitable techniques such as: employing a solvent carrier (including a super critical fluid such as CO.sub.2) or mixtures of solvents that swell the host allowing for diffusion of the hydrophilic guest into its host; treatment of the solvent or a mixture of solvents to make the guest sufficiently ampiphilic for diffusion into the hydrophobic host (e.g., using an HA ester); or simultaneous synthesis of host with a guest having a solvent carrier or having been otherwise treated (e.g., thermally molding UHWMPE in the presence of HA treated to make it temporarily hydrophobic to prevent phase separation then cross-linking simultaneously). For further details of novel diffusion techniques, see ATTACHMENT A. A diffusion profile of the IPN, with its gradual concentration of guest from the bearing outer surface a depth, d, provides structural integrity of the bearing surface and its associated structure by removing the sharp change in modulus inherent in superficially coating or grafting a surface according to known techniques. Cross-linking to finally produce the IPN can be done by employing chemical techniques such as that used by the applicants in earlier work (see that reference above describing the synthesis of a sequential IPN of an UHMWPE host polymer and poly-L-lysine (PLL) guest using silylation), or done employing a technique using another form of energy such as thermal energy or irradiation using a higher-energy source such as UV (ultraviolet) radiation.

(34) If it is an object to produce an outer layer comprised of an entanglement of host and hydrophilic polymer guest creating a bio-compatible bearing surface exhibiting lubricity, one can fabricate such a bearing structure comprising a hydrophobic host (such as UHMWPE) and a hydrophilic polymer guest (such as hyaluronic acid, HA, or other polyion). By way of example, the layer can be synthesized by, first, diffusing the hydrophilic guest (HA) into the hydrophobic host whereby the hydrophilic guest is temporarily made sufficiently hydrophobic to generally prevent phase separation thereof until being cross-linked, thus creating the entanglement. The background materials labeled ATTACHMENT A may be of further guidance: the guest diffuses into the surface of the host creating a diffusion profile extending a depth, d, from the bearing outer surface, throughout which a concentration gradient of cross-linked guest entangled within polymer host is created. The cloaking of the hydrophilic groups of the guest by silylation along with a swelling of the host, provides room within the host for the guest to diffuse into the host so the guest can be cross-linked with itself, into place. As explained, cloaking groups are then removed by hydrolysis to return the guest to its hydrophilic nature.

(35) Cross-linking to produce the entanglement can be done by employing chemical techniques (e.g., as done in the earlier synthesis by the applicants of a sequential IPN of an UHMWPE and poly-L-lysine (PLL) guest using silylation), or done employing a technique using another form of energy such as thermal energy or irradiation using a higher-energy source such as UV (ultraviolet) radiation.

(36) Hyaluronic acid (HA) is a polysaccharide native to synovial fluid, capable of being crosslinked and possesses exceedingly lubricious, and very hydrophilic characteristics. The quick biodegradation of HA (hydrolytic and enzymatic) severely limits its application and use as a wear or friction surface where higher-stresses are experienced, including bearing structures used in: biomedical applications as joint replacements, tissue-joint pads, or other implants; sensitive aqueous environments such as drinking/potable water facilities; and food-preparation or pharmaceutical manufacturing equipment (where toxicity is a concern).

Example 1

(37) The following technical discussion is presented by way of example, only, and concerns the synthesis of an entanglement, such as an IPN, into bearing structures according to the invention (see, also, detailed technical discussion found in ATTACHMENT A):

(38) A. Silylation of HA:

(39) The hydroxyl and acetamido groups in HA must be silylated to make them more hydrophobic so that the HA will dissolve in a solvent which swells UHMWPE. Preferred criteria for the silylation agent are: (1) effectively silylate the HA without complex reaction conditions; (2) should not produce insoluble byproducts so that the reaction product is easily purified; (3) the bonding between Si and O strong enough to resist cleavage by moisture in air during silylation and swelling, and weak enough so that it can hydrolyze under certain conditions (allowing return of HA to hydrophilic state after the synthesis is complete).

(40) The following silylation agents will be measured against the above criteria: N, O-bis(trimethylsilyl)acetamide (BSA), bis(trimethylsilyl)trifluoroacetamide (BSTFA), trimethylchlorosilane (TMCS), and ethyldimethylchlorosilane. The reaction conditions may be those used with the PLL silylation procedures according to applicants prior work, although preferably reaction temperatures will be kept as low as possible (<50 C.) to avoid HA thermal degradation. The reaction products of the silylation agents are the formation of trimethylsilyl ethers, which are extremely prone to hydrolysis. Preferably, the IPN is synthesized without air.

(41) B. Solvent and Crosslinking Agent Selection:

(42) Earlier, the applicants' identified that xylenes yield the highest degree of swelling of UHMWPE. Xylenes will be the solvent used for the IPN synthesis in this example. Methylene chloride and 1,1,1-trichloroethane swell UHMWPE almost as well as xylenes. Thus, each of these solvents will be tried (xylenes, Methylene chloride, and 1,1,1-trichloroethane) to solvate the silylated HA and crosslinker.

(43) The crosslinker criteria include: (1) the crosslinkers are preferably soluble in the solvent used, whether xylenes, methylene chloride or 1,1,1-trichloroethane; (2) the crosslinking reaction preferably occurs between the hydroxyl groups of HA, leaving the carboxyl groups alone for recruiting synovial fluid to decrease friction; and (3) the crosslinked HA is preferably stable in the physiological environment in which bearing structure is used. Glutaraldehyde (GA) and di- or poly-isocyanates are crosslinker candidates. Known uses of glutaraldehyde include use in the crosslinking of proteins such as collagen. GA not only interacts with amino groups (as it is commonly used), but can also react with hydroxyl groups of polysaccharide in aqueous solution or organic solvent such xylene under acidic conditions. The aldehyde groups of GA presumably react with the hydroxyl groups of HA to produce hemiacetalization or full acetalization. Regarding the use of di-isocyanates to crosslink HA coatings: The polysaccharide molecules are covalently bonded by periodic urethane links.

(44) C. Ex Situ Crosslinking:

(45) To verify that HA can be crosslinked by the selected crosslinker in the swelling solvent, and to determine the effect of trimethylsilyl or ethyldimethysilyl groups on the crosslinking, one can perform ex situ crosslinking and examine the crosslinked products. Here, silylated HA will be dried and solvated with the selected solvents, and then be transferred to a glass tube containing the crosslinker. Crosslinked gels will be allowed to react until several hours after the last visual change. After the gels have dried, water is added to cause hydrolysis and liberate of the trimethylsilyl or ethyldimethysilyl groups. Preferably, an investigation of various crosslinking concentrations is done so that swelling ratios of the formed gels can be compared to assess the degree of crosslinking for each concentration.

(46) D. IPN Synthesis:

(47) IPN synthesis is performed using the solvent, silylation agent, and crosslinker selected. The swell times and swelling temperatures will be chosen based on what the HA can withstand. While HA is extremely susceptible to thermal degradation in the presence of water, once silylated and dissolved in an air-free organic solvent it may withstand higher temperatures. Of interest are the temperatures at which silylation, swelling and crosslinking occur; to assess, one can look to how much the viscosity of the HA solutions decrease at various temperatures. It is anticipated that only during silylation will the temperatures have to be kept below 50 C., but during the other portions of the synthesis temperatures of 50-70 C. may be acceptable.

(48) For reference purposes, the following additional definitions are offered: Ophthalmic lenses refers to contact lenses (hard or soft), intraocular lenses, eye bandages and artificial corneas, lenses may be placed in intimate contact with the eye or tear fluid, such as contact lenses for vision correction. Hydrophilic, as used herein, describes a material or portion thereof which will more readily associate with water than with lipids. A hydrophilic surface, as used herein, refers to a surface which is more hydrophilic (i.e., more lipophobic) than the bulk or core material of an article. Thus, an ophthalmic lens having a hydrophilic surface describes a lens having a core material having a certain hydrophilicity surrounded, at least in part, by a surface which is more hydrophilic than the core. Polyion or polyionic material, as used herein, refers to a polymeric material including a plurality of charged groups, which includes polyelectrolytes, p- and n-type doped conducting polymers. Polyionic materials include both polycations (having positive charges) and polyanions (having negative charges). A polymer is a molecule built up by repetitive chemical union or bonding together of two or more smaller units called monomers. Polymer includes oligomers, which have two to about 80 monomers, and polymers having more than 80 monomers. A polymer can be linear, branched network, star, comb, or ladder types of polymer; polymers may be synthetic, naturally-occurring or semi-synthetic.

(49) A polycation is a polymer containing a net positive charge, for example poly-L-lysine hydrobromide. While a polycation can contain monomer units that are charge positive, charge neutral, or charge negative, however, the net charge of the polymer is positive. A polycation also can mean a non-polymeric molecule that contains two or more positive charges. A polyanion is a polymer containing a net negative charge, for example polyglutamic acid. While a polyanion can contain monomer units that are charge negative, charge neutral, or charge positive, however, the net charge on the polymer is negative. A polyanion can also mean a non-polymeric molecule that contains two or more negative charges. Nucleic acid refers to a polymer containing at least two nucleotides (which contain a sugar deoxyribose (DNA) or ribose (RNA), a base, and a phosphate group).

(50) UHMWPE is a polyethylene having an estimated weight average molecular weight in excess of about 400,000, usually 1,000,000 to 10,000,000 as defined by a melt index (ASTM D-1238) of essentially zero and a reduced specific viscosity greater than 8, preferably 25-30. A porous UHMWPE preform may be used as the host and/or base material in a system of the invention that includes an outer layer. Preform, as used here, refers to a shaped article which has been consolidated, such as by ram extrusion or compression molding of UHMWPE resin particles into rods, sheets, blocks, slabs or the like. Preforms may be obtained or machined from commercially available UHMWPE, for example GUR 4150 HP ram extruded UHMWPE rods from PolyHi Solidur (Fort Wayne, Ind.). Silane crosslinked UHMWPE has been used for components of total hip replacements. Other modifications of UHMWPE, include: reinforcement with carbon fibers; and post-processing treatments such as solid phase compression molding. The crosslinking of polymers may be either non-ionic (e.g., covalent) or ionic crosslinking. Ions used to ionically crosslink the polymers are polyions and may be anions or cations depending on whether the polymer is cationically or anionically crosslinkable.

Example 2

(51) By way of further examples the following is offered: In the case of using a guest of HA, which is strongly hydrophilic with its many polar groups (COOH, OH and CONHCH.sub.3) on its long molecular chain, diffusion of HA molecules directly into a bulk UHMWPE structure is difficult. Therefore a modification of the HA molecules is done to increase hydrophobicity and compatibility with both UHMWPE and organic solvents used in connection with cloaking.

(52) 2A) Silylation of HA to Increase its Hydrophobicity:

(53) Silylation is a known technique for increasing hydrophobicity, and createorganic-soluble derivatives of substances. During a silylation reaction of HA, the hydrophilic groups containing active hydrogen, such as COOH, OH, and NH.sub.2, are masked by hydrophilic silyl groups. The reaction is reversible, the silylated functional groups can be returned to their original state through hydrolysis reaction. HA is a muco-polysaccharide of molecular weight up to millions (10.sup.6). Compared with silylation of poly-L-lysine (MW=1000), silylating HA is difficult due to its large molecular weight. In contrast to PLL silylation previously performed by the applicants (see above), preferably HA is modified before silylation to increase its solubility in silylation solvents (polar organic solvents can be used). The steps include: (1) Reaction of HA with long-chain aliphatic quaternary ammonium salts (QN.sup.+). Polyanions, such as HA, combined with certain organic cations, such as paraffin chain ammonium (QN.sup.+) ions, produces a precipitable complex. The complex is a true salt of the polyacid and quaternary base. HA was modified with long-chain aliphatic ammonium salts, to improve its solubility in organic solvents. Combination of QN+ with polyanions occurs in those pH ranges in which the polyanions are negatively charged. The reaction between HA and ammonium cations in water can be expressed:
HA.sup.-Na.sup.++QN.sup.+A.sup..fwdarw.HA.sup.-QN.sup.++Na.sup.+A.sup. where HA.sup.-Na.sup.+ is the sodium salt of hyaluronic acid; HA.sup.-QN.sup.+ is the precipitable complex between HA carboxylic polyanion and long chain paraffin ammonium cations. HA.sup.-QN.sup.+ (HA-CPC/HA-CTAB) complexes were used. The complexes (HA.sup.-QN.sup.+) precipitated from HA aqueous solution are soluble in concentrated salt solutions, so HA can be recovered from its insoluble complexes. Ammonium salts used were: cetyltrimethylammonium bromide monohydrate (MW: 358.01) (CTAB) and cetylpyridinum chloride (M.W. 364.46) (CPC). (2) Silylation of HA.sup.-QN.sup.+ complexes: HA-CPC and HA-CTAB were silylated in DMSO solution with BSA, HMDS and other typical silylation agents. Silylation agents are generally sensitive to humidity, silylating operation should be under the purge of dry N.sub.2.

(54) 2B) Acylation of HA to Improve its Thermal Flow:

(55) To make HA flowable at high temperature, the strong hydrogen bonding between its molecules must be disrupted, and the molecular order (i.e., crystallinity) of HA needs to be destroyed. Acylating the hydroxyl groups on HA with long-chain aliphatic carboxylic acids chloride will help in de-crystallizing HA. Acid chlorides, from caproyl to stearoyl chloride, can be used as acylating agents. Acylation is a known process for disrupting crystallinity in other polysaccharides. Acylation reactions are performed in solution (of HA.sup.-QN.sup.+ in DMSO, for example). Start with a DMSO solution of HA.sup.-QN.sup.+ complex using the technique described above in connection with Silylation of HA, above. Acylation is done to make the hydrophilic guest hydrophobic enough to be molded with a hydrophobic host, such as UHMWPE, without phase separation.

(56) 2C) Entanglement by Swelling of Host to Facilitate Diffusion of Guest:

(57) (a) Swell UHMWPE bulk samples from bar stock or molding in an xylene solution of silylated HA.sup.-QN.sup.+ complex. The swelling may be performed at 70 C. for about two weeks. The micro-voids created with swelling in the amorphous region of UHMWPE will allow silylated HA.sup.-QN.sup.+ complex to diffuse in. (b) Crosslink the silylated HA.sup.-QN.sup.+ molecules that have diffused into UHMWPE in situ with di-/poly-isocyanate, such as 1, 8-diisocyanatooctane. The reaction was performed in isocyanate xylene solution. (c) Deswell the samples to remove the xylenes residues. (d) Remove the trimethylsily groups with hydrolysis to recover the hydrophillic OH and CH.sub.3COONH groups. (e) Remove the long-chain paraffin ammonium ions (QN.sup.+) in concentrated salt solution to recover COOH groups. QN.sup.+ will dissociate from COO.sup. groups on HA in concentrated salt solution, such as NaCl, KCl, NaSO.sub.4, etc. Several 0.4N NaCl washings may be necessary for sufficient removal of QN.sup.+.

(58) 2D) Entanglement by Using Porous (e.g., UHMWPE) Host Structure:

(59) (a) Mold UHMWPE powders to produce a preform with controlled porous density (pore size about 20 microns, porosity around 30%). (b) Soak the preform with xylene solution of silylated HA-QN.sup.+ complex (e.g., approx. one hour). The pores, whether interconnected, allow HA molecules to diffuse into UHMWPE. (c) Crosslink the HA-QN.sup.+ inside UHMWPE preform with an appropriate crosslinker such as, but not limited to, a di-/polyisocyanate. Note: select a solvent that will not solvate the silylated HA-QN+. (d) May perform a light molding (do not mold to fully dense) to aid in physically trapping the HA guest inside the UHMWPE porous structure. (e) Recover COOH, OH and CH.sub.3COONH groups on HA. (f) (Re)mold and size the treated pre-form to final shapekeep temperature below that which will degrade HA.

(60) 2E) Entanglement by Powdered Mixture which can be Molded:

(61) HA can form entanglement with UHMWPE during the procedure of molding their powder mixture. The strong hydrophibicity from acylation of HA aids in making HA compatible with UHMWPE so that phase separation does not occur. (a) Mold UHMWPE bulk under the standard molding cycles into a slightly undersized base structure depending upon application of resulting device. (b) On the surface of this undersized molded bulk UHMWPE device/object, put a thin layer of powered HA-crosslinker-UHMWPE mixture (preferably cloaked using acylationsee above 2B), and then mold it to a selected shape and size. (c) Recover the hydrophillic functional groups on HA.

Example 3

(62) By way of further examples the following is offered:

(63) The porous structure of the hydrophobic host(s) can be created by sintering (i.e. molding with a low pressure and no porogen), or by using a porogen and molding. During sintering, the porous structure of the porous hydrophobic host can be specified by controlling sintering variables, e.g. powder size, powder shape, powder size distribution, sintering time, temperature and pressure. The porous hydrophobic host can also be formed via a porogen method. Porogens are added to a powder form of the hydrophobic host, which is then directly compression molded; the porogen(s) create pores by first acting as place holder, then are leached out (or incinerated) of the compression molded hydrophobic host leaving behind pores. The shape and size of the porogen directly correlates to the shape and size of the pores, and the amount of porogen added to the material is related to the final percent porosity of the hydrophobic host. In the fabrication of the material (or elsewhere herein, micro-composite), the hydrophobic host and the functionalized hydrophobic host powders are uniformly blended together before making the porous structure. Before further treatment with the hydrophilic guest and final molding, the porous hydrophobic host may be annealed, quenched and/or thermally treated in other ways.

(64) After infiltration of the silylated ammonia salt derivative of the hydrophilic guest, the silylated ammonia salt derivative of the hydrophilic guest is crosslinked in situ then put through a hydrolysis process to return the silylated ammonia salt derivative of the hydrophilic guest to its hydrophilic native state (converting from a hydrophobic derivative guest to a hydrophilic guest). The treated porous hydrophobic host is then surface treated with the hydrophilic guest; the hydrophilic guest is then crosslinked in situ. At this point those samples without the additional step of host/co-host crosslinking (see FIG. 5A at 67, setting forth the step dope w/PE (Host/co-Host) crosslinker and an antioxidant) are ready for compression molding; however, the hydrophobic host portion of those samples with the additional, optional step of host/co-host crosslinking (FIG. 5A at 67) are treated simultaneously with a chemical crosslinker, either a silane or a peroxide (e.g. dicumyl peroxide [(bis(1-methyl-1-phenylethyl) peroxide], and benzyl peroxide [2,5-Dimethyl-2,5-di-(tert-butyl-peroxy) hexyne-3 peroxide], 2,5-dimethyl-2,5-bis(tert-butylperoxy)-3-hexyne.sup.7), andlikewise, optionallyan antioxidant, such as a phenolic antioxidant (e.g. Irganox 1076 [stearyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate], or vitamin E (-tocopherol)) via treatment with a solution containing the crosslinker and antioxidant (e.g. chemical crosslinker and antioxidant dissolved in acetone) and subsequent evaporation of the solvent (i.e. acetone). The presence of an antioxidant traps free radicals, disabling further oxidation or HA degradation. At this point all sample groups are ready for compression molding (which will amount to a re-molding if molding was used to make initial porous structure).

(65) While certain representative embodiments and details within EXAMPLES have been shown merely for the purpose of illustrating the new layer material for forming into structures and associated technique of synthesizing same, those skilled in the art will readily appreciated the various modifications may be made to these representative embodiments without departing from the novel teachings or scope of this technical disclosure. Accordingly, all such modifications are intended to be included within the scope of this invention as defined in any illustrative-claim included below. Although the commonly employed preamble phrase comprising the steps of may be used herein, or hereafter, in a method claim, the Applicants do not intend to invoke 35 U.S.C. Section 112 6 in a manner that unduly limits claim scope. Furthermore, in any claim that is filed herewith or hereafter, any means-plus-function clauses used, or later found to be present, are intended to cover at least all structure(s) described herein as performing the recited function and not only structural equivalents but also equivalent structures.