ELECTROLYZED WATER COMPOSITION

Abstract

The present invention provides a method of treating wheat fungal pathogens, comprising applying an electrolyzed water composition to a wheat crop affected with pathogens or area containing a wheat crop affected with pathogens. The electrolyzed water composition is prepared by a method comprising: preparing an electrolyte solution comprising water, at least one carbonate salt selected from anhydrous alkali metal carbonate salts, and at least one chloride salt selected from: alkali metal chloride salts; introducing the aqueous electrolyte solution into an electrolytic cell comprising a plurality of boron-doped diamond electrodes; and operating a power supply to apply a predetermined voltage to the electrolyte solution to produce an electrolyzed water biocidal composition comprising a plurality of active molecular and ionic species having biocidal activity, in which the mixture of at least two salts of the electrolyte are selected such that the dissolved O.sub.3 concentration is in the range of from 0.1 to 1,000 ppm.

Claims

1. A method of treating wheat fungal pathogens, comprising applying an electrolyzed water composition to a wheat crop affected with pathogens or area containing a wheat crop affected with pathogens, in which the electrolyzed water composition is prepared by a method comprising: preparing an electrolyte solution comprising water, at least one carbonate salt selected from anhydrous alkali metal carbonate salts, and at least one chloride salt selected from: alkali metal chloride salts; introducing the aqueous electrolyte solution into an electrolytic cell comprising a plurality of boron-doped diamond electrodes; and operating a power supply to apply a predetermined voltage to the electrolyte solution to produce an electrolyzed water biocidal composition comprising a plurality of active molecular and ionic species having biocidal activity, in which the mixture of at least two salts of the electrolyte are selected such that the dissolved O.sub.3 concentration is in the range of from 0.1 to 1,000 ppm.

2. A method as claimed in claim 1, comprising applying the electrolyzed water composition is applied to the wheat crop or area containing a wheat crop using an applicator selected from one or more of a nebuliser, a fogging mist applicator, a jet spray applicator, a spray applicator, an irrigation system, or any combination thereof.

3. A method for treatment of wheat fungal pathogens, in which the electrolyzed water composition is prepared by a method comprising: preparing an electrolyte solution comprising water, at least one carbonate salt selected from anhydrous alkali metal carbonate salts, and at least one chloride salt selected from: alkali metal chloride salts; introducing the aqueous electrolyte solution into an electrolytic cell comprising a plurality of boron-doped diamond electrodes; and operating a power supply to apply a predetermined voltage to the electrolyte solution to produce an electrolyzed water biocidal composition comprising a plurality of active molecular and ionic species having biocidal activity, in which the mixture of at least two salts of the electrolyte are selected such that the dissolved O.sub.3 concentration is in the range of from 0.1 to 1,000 ppm.

4. The method of claim 3 wherein, the electrolyte solution comprises at least one carbonate salt selected from anhydrous sodium carbonate and/or anhydrous potassium carbonate.

5. The method of claim 3 wherein, the electrolyte solution comprises at least one chloride salt selected from sodium chloride and potassium chloride.

6. The method of claim 3 wherein, the electrolyte solution comprises at least one carbonate salt selected from anhydrous alkali metal carbonates, and at least one chloride salt selected from alkali metal chloride salts.

Description

BRIEF DESCRIPTION OF FIGURES

[0036] Embodiments of the present invention will now be described, by way of example, with reference to the following figures:

[0037] FIG. 1 is a graphical representation of the life cycle of Septoria tritici;

[0038] FIG. 2a-2f are photographic representations comparing the effect of applying the compositions of Example 1 (labelled Mix38 and K38), and a comparative electrolyzed water composition (labelled SD); and known pesticide Adexar to wheat plants infected with Septoria tritici;

[0039] FIG. 3 is a graphical representation comparing the effect measured in terms of disease score of applying the compositions of Example 1 (labelled Mix38 and K38), and a comparative electrolyzed water composition (labelled SD); and known pesticide Adexar to wheat plants infected with Septoria tritici; and

[0040] FIG. 4 is a graphical representation comparing the effect measured in terms of leaf length of applying the compositions of Example 1 (labelled Mix38 and K38), and a comparative electrolyzed water composition (labelled SD); and known pesticide Adexar to wheat plants infected with Septoria tritici.

DETAILED DESCRIPTION

Example 1Electrolyzed Water Composition

[0041] An aqueous electrolyte solution comprising 14 g sodium chloride and 16 g anhydrous sodium carbonate in 12 l of water was prepared. The electrolyte solution was stored within a reservoir chamber in fluid communication with an electrolytic cell.

[0042] A feed stream comprising the electrolyte solution was introduced into an electrolytic flow cell. The feed stream can optionally include one or more additional salts to enhance the biocidal properties of the resultant electrolyzed water composition.

[0043] The electrolytic cell is a non-membrane electrolytic cell. It is however to be understood that any suitable electrolytic cell may be used.

[0044] The electrolytic cell comprises a casing, a plurality of boron doped diamond electrodes (BDEs) located within the cell, and metal contact plates used for transmitting charge across the electrolyte solution.

[0045] The BDEs are sheet-like components and are provided in a stack of between 3 and 10 sheets. Each sheet is located at a fixed distance away from an adjacent sheet. The distance between adjacent sheets of BDEs provides a cell gap, which is preferably less than 5 mm, for example between approximately 2 and 3 mm. The BDEs are provided in a plastic frame. The BDEs transmit charge across the electrolyte solution, inducing a strong dipole and creating positively and negatively charged radicals on alternate surfaces of the diamonds.

[0046] The electrolyte solution may be introduced into the electrolytic cell in any suitable manner so as to produce electrolyzed water composition in a continuous process or in a batch process. In the continuous process, the electrolyte solution may be introduced at a suitable flow rate, such as for example at a flow rate in the range of from 0.1 to 100 l/min, for example in the range of from 3 to 5 l/min. In the batch process, the electrolyte solution may have a flow rate of approximately 16 l/min.

[0047] A power supply was operated to apply a voltage in the range of between 1 and 1,000 Volt D.C. and a current within the range of from 1-1,000 ampere to the electrolyte solution.

[0048] The over-potential provided between the electrodes shifts the equilibrium within the electrolyte solution such that a range of active species ions and molecules are produced and remain within the electrolyzed water for a significant amount of time. The term significant amount of time is used herein to refer to at least 10 minutes, preferably at least 30 minutes, more preferably at least 45 minutes, for example at least 60 minutes. The combination of active molecular and ionic species together with the over-potential which supports the equilibrium confers a variable degree of pesticidal activity to the electrolyzed water composition.

[0049] The electrolytic cell preferably comprises an outlet through which the electrolyzed water composition exits the cell. The resulting electrolyzed water composition comprises a range of active molecular and ionic species which have biocidal properties.

[0050] The active molecular and ionic species include dissolved ozone. The electrolyzed water composition according to this embodiment comprises dissolved ozone at a level of approximately 50 ppm. The electrolyzed water composition according to this embodiment comprises free accessible chlorine (FAC) at a level of approximately 350 ppm.

[0051] Although the present invention comprises the use of electrolyzed water compositions containing dissolved ozone at a level of approximately 50 ppm, it is to be understood that the present invention may use electrolyzed water compositions comprising any suitable level of dissolved ozone within the range of between 0.1 and 1,000 ppm. Although the present invention comprises the use of electrolyzed water compositions containing FAC at a level of approximately 350 ppm, it is to be understood that the present invention may use electrolyzed water compositions comprising any suitable level of FAC within the range of between 0 and 1,000 ppm, for example between 0.01 and 350 ppm.

[0052] It is also to be understood that the electrolyzed water composition may be varied by varying one or more of: the components of the electrolyte composition, the concentration of the components within the electrolyte composition, the degree of over-potential, the current applied, or any combination thereof. In this way the biocidal properties of the electrolyzed water biocidal composition may be tailored to suit different agricultural targets, such as for example crops, pathogens, delivery mechanism, and time points, or any combination thereof. For example, the biocidal properties of the electrolyzed water biocidal composition may be tailored in relation to when the composition is to be applied, such as for example during preparation of growing beds, during sowing and/or during growing seasons.

[0053] The system may further comprise one or more flow regulators arranged in use to adjust the flow of the electrolyte feed stream between the reservoir and the cell.

[0054] The system may further comprise a heater arranged in use to adjust the temperature of the flow of the electrolyte feed stream and/or the electrolyte solution within the cell.

[0055] The system may further comprise a control system arranged in use to control the flow rate of the electrolyte feed stream as required, such as for example by controlling the flow regulator(s).

[0056] The system may comprise a control system arranged in use to control the power supply to the electrodes.

[0057] The system may comprise a control system arranged in use to control the temperature of the electrolyte solution.

[0058] Control of the temperature of the electrolyte solution, the flow rate of the electrolyte solution feed stream, and the power supply to the electrodes may be provided by a single control system. Alternatively, these factors may be controlled by separate control systems.

Example 2Septoria tritici (Mycosphaerella graminicola) Control on Wheat Plants

[0059] Septoria tritici infected wheat plants were treated with five different treatments.

[0060] Treatment 1: untreated control (UT);

[0061] Treatment 2: Adexar (known fungicide);

[0062] Treatment 3: SD (Comparative Example of an alternate electrolysed water solution, with salts comprising NaCl at 0.30 g/l, Na.sub.2CO.sub.3 at 1.60 g/l, KH.sub.2PO.sub.4 at 0.90 g/l, KNO.sub.3 at 0.80 g/l, CaCl.sub.2).6H.sub.2O at 1.60 g/l, Mg(NO.sub.3).sub.2.6H.sub.2O at 0.80 g/l);

[0063] Treatment 4: composition of Example 1 with sodium salts (Mix 38).

[0064] Treatment 5: composition of Example 1 with potassium salts (K38)

[0065] The treatments were applied using a foliar spray. Each treatment group consisted of four replicates of 8 plants from a susceptible variety of wheat (Consort; Gallant; and Riband). Each treatment was sprayed onto the diseased plants for 10 seconds. It is to be understood that the treatment is to be applied until the treatment begins to run off from the leaves.

[0066] The results of the treatment are illustrated in FIG. 2A to 2E, FIG. 3 and FIG. 4.

[0067] FIGS. 2A to 2E are photographic images of wheat plants infected with Septoria tritici (Mycosphaerella graminicola). The wheat plants shown in FIG. 2A are not treated with any pesticidal composition (treatment 1). The wheat plants shown in FIG. 2B are treated with a known pesticidal composition known as Adexar (treatment 2). The wheat plants shown in FIGS. 2D and 2E are treated with the composition of Example 1 (treatments 4 and 5).

[0068] FIG. 2A shows that the untreated wheat plants are diseased by the plant pathogens. A significant number of the leaves are browning, wilting and diseased.

[0069] As shown in FIG. 2B, the wheat plants treated with Adexar (treatment 2) appear significantly more healthy than the untreated wheat plants (treatment 1) of FIG. 2A. The wheat plants treated with Adexar have less wilting and diseased branches and leaves. This illustrates that Adexar is effective at treating at least some of the plant pathogens under idealised conditions.

[0070] As shown in FIGS. 2D and 2E, the wheat plants treated with the composition of Example 1 (treatments 4 and 5) are significantly healthier than the untreated plants of FIG. 2A (treatment 1), and almost as healthy as the plants treated with Adexar (treatment 2) (FIG. 2B). The wheat plants treated with the composition of Example 1 (treatments 4 and 5) appear to have very few wilting or diseased leaves and branches.

[0071] The effect of resistance to pesticides is well known and various wheat fungicides products in classes such as triazols and succinate dehydrogenase inhibitors (SDHIs) suffer from reduced efficacy due to development of pesticide resistance in the pathogen population. The active ingredient in the electrolyzed water composition of Example 1 has a physicochemical mechanism of action for which no resistance has been observed. The electrolyzed water composition of Example 1 therefore has a similar pesticidal effect against Septoria tritici (Mycosphaerella graminicola) in wheat crops than the known pesticide Adexar, but offers a non-resistance generating alternative treatment option.

[0072] FIG. 3 illustrates the degree of wheat crop infection or disease as represented by the percentage of leaves displaying wilting and/or formation of pale brown lesions on the bottom leaves with small black fungal bodies (pycnidia) as a factor of time after treatment. It can be seen that the use of the compositions of Example 1 (Treatments 4 and 5; Mix38 and K38) provides an improved pesticidal effect and significantly reduces the percentage of disease on the plants when compared with the untreated control (treatment 1: UT) and the other electrolyzed water composition treatments. Treatments 4 and 5 (Composition of Example 1) perform almost as well as the known pesticide (Treatment 2: Adexar).

[0073] FIG. 4 illustrates the total leaf length as a factor of time after treatment. It can be seen that the use of the compositions of Example 1 (Treatments 4 and 5: Composition of Example 1) provides an improved pesticidal effect and does not adversely affect the crop growth when compared with the untreated control (treatment 1: UT) and the two other treatments.

[0074] The method of pesticidal treatment of a substrate, for example wheat crops or areas comprising wheat crops, using the electrolyzed water compositions described herein have significantly reduced environmental issues compared to conventional methods. In contrast to a number of conventional methods, the electrolyzed water compositions described herein contain only simple, non-toxic and food-approved salts. Use of the electrolyzed water compositions described herein are therefore more environmentally friendly than known pesticidal compositions. Furthermore, use of the compositions described herein does not leave any harmful chemical residues on treated food. The electrolyzed water compositions described herein are non-toxic and non-tainting. The electrolyzed water compositions described herein have a significantly improved ozone concentration compared to the level which can be achieved by injection of gaseous ozone into water. For example, the electrolyzed water compositions described herein may have approximately 100 times the level which can be achieved by injection of gaseous ozone into water. As such, the wheat crops may be treated by the electrolyzed water compositions described herein more frequently, during extended periods of crop production, such as for example closer to crop harvest, and without requiring any additional health and safety protection or equipment. The present invention provides a cost effective alternative to the use of known chemical pesticides for the treatment of wheat fungal pathogens. Use of the electrolyzed water compositions as described herein for the treatment of wheat fungal pathogens provides medium term protective effect and an ongoing protective effect.

[0075] It is to be understood that the Examples are illustrative of the pesticidal properties of the compositions of the present invention. It is to be understood that the compositions described herein may be applied in any suitable manner to an agricultural area or crop(s) comprising wheat crops.