MOISTURIZING COMPOSITION AND APPLICATION THEREOF IN PREPARATION OF MOISTURIZING COSMETIC PRODUCT

Abstract

A moisturizing composition and an application thereof in the preparation of a moisturizing cosmetic product; each component in the formulation cooperates with the others to jointly provide the effects of increasing the moisture content of the skin and reducing the loss of moisture of the skin; compared with the efficiency of using a single component or several components alone, the efficiency of the present invention is significantly increased, indicating that compounding each component at a proper ratio produces a synergistic effect, thereby improving moisturizing efficiency.

Claims

1. A composition, consisting of the following ingredients in parts by mass: TABLE-US-00015 Glycerol 2~6 parts; Butanediol 2~6 parts; Propanediol 1~4 parts; Betaine 1~4 parts; Allantoin 0.05~0.5 part; Trehalose 0.01~2 part; Oat glucan 0.01~2 part; Xylitol 0.01~2 part; Hyaluronic acid 0.01~0.5 part; Oligo hyaluronic acid 0.01~0.5 part; Chitosan oligosaccharide 0.01~1 part; Tremella polysaccharide 0.01~1 part; Nicotinamide 1~3 parts; Amino acid 0.1~3 parts; Repairing peptides 0.01~0.05 part; Panthenol 0.05~0.5 part; Ceramide liposome 0.01~0.5 part.

2. The composition according to claim 1, wherein the ceramide liposome is made from the following raw materials in parts by mass: TABLE-US-00016 Ceramide 3~8 parts; Caprylic/capric triglyceride 6~9 parts; Candelilla wax 14~21 parts; Hydrogenated lecithin 4~6 parts; Cholesterol 2~4 parts; Alcohol 5~7 parts; Tween-60 5~6 parts; Glycerol 8~12 parts; Water 27~53 parts.

3. The composition according to claim 2, wherein the ceramide liposome is prepared by a method comprising: at 90 C., mixing ceramide, caprylic/capric triglyceride, candelilla wax, hydrogenated lecithin, cholesterol and alcohol, and dissolving to obtain an oil phase; mixing Tween, glycerol and water to obtain an aqueous phase; and at 90 C., mixing the oil phase with the aqueous phase, emulsifying at 5000 rpm for 5 min, homogenizing at 1000 bar for 3 times, filtering and drying to obtain the ceramide liposome.

4. A method for producing a moisturizing cosmetic, comprising adding the composition according to claim 1 in the moisturizing cosmetic.

5. A moisturizing cosmetic, comprising the composition according to claim 1.

6. The moisturizing cosmetic according to claim 5, wherein the mass fraction of the composition is 7.33%38.05%.

7. The moisturizing cosmetic according to claim 5, further comprising Carbomer U-20, arginine, preservative and fragrance.

8. The moisturizing cosmetic according to claim 5, comprising: TABLE-US-00017 Glycerol 2%~6%; Butanediol 2%~6%; Propanediol 1%~4%; Betaine 1%~4%; Allantoin 0.05%~0.5%; Trehalose 0.01%~2%; Oat glucan 0.01%~2%; Xylitol 0.01%~2%; Hyaluronic acid 0.01%~0.5%; Oligo hyaluronic acid 0.01%~0.5%; Chitosan oligosaccharide 0.01%~1%; Tremella polysaccharide 0.01%~1%; Nicotinamide 1%~3%; Amino acid 0.1%~3%; Repairing peptides 0.01%~0.05%; Panthenol 0.05%~0.5%; Ceramide liposome 0.01%~0.5%; Carbomer U-20 0.05%~0.5%; Arginine 0.05%~0.5%; Preservative 0.8%~1.2%; Fragrance 0.01%~0.03%; and Balance water.

9. A method for producing the moisturizing cosmetic according to claim 6, comprising, at 8085 C., mixing water, glycerol, butanediol, propanediol, betaine, allantoin, trehalose, oat glucan, xylitol, hyaluronic acid, oligo hyaluronic acid, chitosan oligosaccharide and tremella polysaccharide, stirring at 60 rpm for 30 min, and homogenizing at 8000 rpm for 3 min to fully dissolve the above ingredients; adding Carbomer U-20 and arginine, stirring at 50 rpm for 25 min, and homogenizing at 5000 rpm for 2 min; reducing the temperature to 4550 C., adding nicotinamide, amino acid, repairing peptides and panthenol, and stirring at 50 rpm for 25 min; and adding ceramide liposome, lecithin, cholesterol, preservative, and fragrance successively, stirring at 50 rpm for 25 min, and cooling to room temperature to obtain the moisturizing cosmetic.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0054] FIG. 1 shows effect of different test samples on the increasing ratio of moisture content of skin along time.

[0055] FIG. 2 shows effect of different test samples on the reducing ratio of skin moisture along time.

DETAILED DESCRIPTION

[0056] The present disclosure provides a composition, and a use thereof in preparing moisturizing cosmetics. One of ordinary skill in the art can learn from the contents of this document and appropriately improve the process parameters. It is to be noted that all such alternatives and modifications are obvious to one of ordinary skill in the art and are considered to be included in the present disclosure. The method and application of the present disclosure have been described in terms of preferred embodiments. It will be apparent to one of ordinary skill in the art that the methods and applications herein may be modified or modified in various ways without departing from the spirit and scope of the disclosure.

[0057] All the test materials used in the present disclosure are common commodities, which can be purchased on the market. The composition provided by the present disclosure contains multi-dimensional moisturizing factors, so called uniHydra.

[0058] The present disclosure will be illustrated in conjunction with examples hereinafter.

Preparing Liposomes of Examples 13

[0059] The formulas are shown in Table 1.

TABLE-US-00010 TABLE 1 Formulas of examples 1~3 Phase Ingredient Example 1 Example 2 Example 3 Oil Phase Ceramide 3 g 8 g 5 g Caprylic/capric 6 g 9 g 7 g triglyceride Candelilla Wax 14 g 21 g 18 g Hydrogenated Lecithin 4 g 6 g 5 g Cholesterol 2 g 4 g 3 g Alcohol 5 g 7 g 6 g Aqueous Tween-60 5 g 6 g 5.5 g Phase Glycerol 8 g 12 g 10 g Water 53 g 27 g 40.5 g

[0060] To prepare the oil phase, ceramide, caprylic/capric triglyceride, candelilla wax, hydrogenated lecithin and cholesterol and alcohol were weighed according to proportions thereof, and heated to 90 C. to fully melt the oil. Thereafter, the mixture was well stirred. The temperature of oil phase was maintained at 90 C., and before emulsification, the lost alcohol was replenished.

[0061] To prepare the aqueous phase, Tween-60, glycerol and water were weighed according to proportions thereof, and heated to 90 C. to make Tween-60 fully dissolved in water. The temperature of the aqueous phase was maintained at 90 C., and before emulsification, the lost water was replenished.

[0062] The temperature of the oil phase was maintained at 90 C., and the 90 C.-aqueous phase was poured into the oil phase. The temperature was maintained at 90 C. A high-speed homogenizer was used to carry out the emulsification, and the condition was 5000 rpm for 5 min. After emulsification, the temperature was still maintained at 90 C.

[0063] Hot water was added to the high-pressure homogenizer firstly, then the feed pipe was heated to a temperature of 90 C. and the pressure was adjusted to 1000 bar. After the water totally entered the machine, the emulsion was added; and the emulsification was carried out at 1000 bar for 3 times.

[0064] The resultant was diluted 100 times and the particle diameter was measured (FIG. 1). The results showed that the average particle diameter was 347.6 nm.

Preparing Cosmetics of Examples 46

[0065] The formulas are shown in Table 2.

TABLE-US-00011 TABLE 2 Formulas of examples 4~6 Phase Ingredient Example 4 Example 5 Example 6 A Water 92.56 g 57.95 g 75.45 g Glycerol 2 g 6 g 4 g Butanediol 2 g 6 g 4 g Propanediol 1 g 4 g 2.5 g Betaine 1 g 4 g 2.5 g Allantoin 0.05 g 0.5 g 0.25 g Trehalose 0.01 g 2 g 1 g Oat glucan 0.01 g 2 g 1 g Xylitol 0.01 g 2 g 1 g Hyaluronic Acid 0.01 g 0.5 g 0.25 g (MW: 2,100,000) Hyaluronic Acid 0.01 g 0.5 g 0.25 g (Oligomerization) Chitosan Oligosaccharide 0.01 g 1 g 0.5 g Tremella Polysaccharide 0.01 g 1 g 0.5 g B Nicotinamide 1 g 3 g 2 g Compound Amino Acid 0.1 g 3 g 2 g Repairing peptides 0.01 g 0.05 g 0.03 g Panthenol 0.05 g 0.5 g 0.25 g C Ceramide liposome 0.01 g 0.5 g 0.25 g D Carbomer U-20 0.05 g 0.5 g 0.25 g Arginine 0.05 g 0.5 g 0.25 g E Caprylhydroxamic Acid 0.8 g 1.2 g 1 g PHL Fragrance 0.01 g 0.03 g 0.02 g

[0066] The process was shown hereinafter.

[0067] 1) The processing water in the reaction kettle was heated to 8085 C., and ingredients of A phase were successively added. The mixture was stirred at 60 rpm for 30 min, and then homogenized at 8000 rpm for 3 min to fully dissolve the related ingredients.

[0068] 2) D phase was added to the reaction kettle. The mixture was stirred at 50 rpm for 25 min and homogenized at 5000 rpm for 2 min to fully dissolve the related ingredients.

[0069] 3) B phase was successively added to the reaction kettle. The mixture was stirred at 50 rpm for 25 min, and then the temperature was reduced to 4550 C.

[0070] 4) C phase and the E phase were successively added. The mixture was stirred at 50 rpm for 25 min, and then the temperature was reduced to room temperature to obtain the cosmetics.

Preparing Cosmetics of Comparative Examples 13

[0071] The formulas are shown in Table 3.

TABLE-US-00012 TABLE 3 Formulas of comparative examples 1~3 Comparative Comparative Comparative Comparative Comparative Phase Ingredients Example 1 Example 2 Example 3 Example 4 Example 5 A Water 75.45 g 75.45 g 75.45 g 75.45 g 75.45 g Glycerol 8.01 g 4.17 g Butanediol 8.01 g 5.57 g Propanediol 5.01 g 2.78 g Betaine 6.77 g 10.47 g 0.42 g Allantoin 0.68 g 1.05 g 0.01 g Trehalose 6.58 g 5.57 g Oat Glucan 6.58 g 2.71 g 0.01 g Xylitol 2.00 g 0.01 g Hyaluronic Acid (MW: 1.65 g 0.01 g 2,100,000) Hyaluronic Acid 1.65 g 0.68 g 0.01 g (Oligomerization) Chitosan Oligosaccharide 3.29 g 2.09 g 0.01 g Tremella Polysaccharide 3.29 g 0.01 g B Nicotinamide 5.42 g 0.01 g Composited Amino Acid 5.42 g 8.37 g 0.01 g Repairing peptides 0.00 g Panthenol 0.68 g 2.92 g C Ceramide liposome 0.68 g 1.05 g 1.53 g D Carbomer U-20 0.25 g 0.25 g 0.25 g 0.25 g 0.25 g Arginine 0.25 g 0.25 g 0.25 g 0.25 g 0.25 g E Caprylhydroxamic Acid PHL 1 g 1 g 1 g 1 g 1 g Fragrance 0.02 g 0.02 g 0.02 g 0.02 g 0.02 g

[0072] The process was shown hereinafter.

[0073] 1) The processing water in the reaction kettle was heated to 8085 C., and ingredients of A phase were successively added. The mixture was stirred at 60 rpm for 30 min, and then homogenized at 8000 rpm for 3 min to fully dissolve the related ingredients.

[0074] 2) D phase was added to the reaction kettle. The mixture was stirred at 50 rpm for 25 min and homogenized at 5000 rpm for 2 min to fully dissolve the related ingredients.

[0075] 3) B phase was successively added to the reaction kettle. The mixture was stirred at 50 rpm for 25 min, and then the temperature was reduced to 4550 C.

[0076] 4) C phase and the E phase were successively added. The mixture was stirred at 50 rpm for 25 min, and then the temperature was reduced to room temperature to obtain the cosmetics.

Efficacy Evaluation

[0077] Efficacy evaluation was performed between the moisturizing cosmetics purchased on the market and the moisturizing cosmetics prepared in the present disclosure. The evaluation was carried out according to the following method.

[0078] 1. Method for Measuring the Moisture Measurement Value (MMV) of Skin

[0079] 1.1 Principle of the Test

[0080] Capacitance method was used to measure the moisture content in stratum corneum of human skin. The principle of the method is that, the dielectric constants of water and other substances were notably different, therefore, if the moisture contents of skin are different, the observed capacitance of skin are different, and the observed value can represent the skin moisture value.

[0081] 1.2 Measurement Equipment

[0082] Multi-functional skin tester (CUTOMETER DUAL MPA 580, CK Company, Germany) was used, and in the experiments, moisture content of skin test probe and skin transepidermal water loss TEWL test probe were used.

[0083] 1.3 Requirements for Measurement Conditions and Volunteers

[0084] Conditions of measurement environment: environment temperature of 221 C., humidity of 505%, and real-time dynamic detection.

[0085] Requirements for the volunteers: at least 30 valid volunteers, 1665 years old (excluding pregnant and lactating women); the baseline of the skin moisture content in the testing region on forearm measured by capacitance method was between 15 and 100; without serious systemic diseases, immunodeficiency or autoimmune diseases; without active allergic diseases; without history of severe allergies to skin care cosmetics; without using hormonal drugs and immunosuppressive agents in the past month. In addition, the volunteers had not participated in other clinical trials during the test period and used the test samples according to regulations, and the information was complete. All volunteers have complete data and signed the informed consent form before the test.

[0086] 1.4 Preparations Before the Test

[0087] 23 days before the test, no product (cosmetics or external drugs) was allowed to apply on the testing region. Before the test, the inner sides of forearms of the subjects were cleaned and wiped with a dry facial tissue. After cleaning, the test regions were marked on the inner side of forearm. Before the measurement, the subjects sit in a room which met the requirement standard for at least 30 minutes without drinking water. Their forearms were exposed as in the test state, and kept relaxed.

[0088] 1.5 Procedure of the Test

[0089] In the test, a testing region of 33 cm.sup.2 was marked on the inner side of the left and right arms, and multiple regions might be marked on the same arm with an interval of 1 cm. The test samples and the blank control were randomly distributed on the left and right arms. Capacitive skin tester was used to perform the measurement on the testing regions and the control regions. Each area was measured 15 times in parallel. The basal value of each testing region was measured first, and then the test sample was evenly applied to the testing region with a latex finger sleeve at a dosage of 2.00.1 mg sample/cm.sup.2. Moisture contents of skin in the testing region and the blank control region were measured after 1 hour, 2 hours and 4 hours (according to this time at the time of verification). The measurement of the same volunteer was performed by the same surveyor.

[0090] 1.6 Analysis of Data

[0091] MMV values of different periods were respectively measured according to the design of the test.

[0092] 1.5 Analysis of Data

[0093] MMV values of different periods were respectively measured according to the design of the test.

[0094] Formula of Calculation:

W %=(W1W0)/W0100%

[0095] Comment: W %=increasing percentage of moisture measurement value (MMV) [0096] W0=moisture content of skin before using the sample [0097] W1=moisture content of skin after using the sample

[0098] The results were shown in Table 4 and FIG. 1

TABLE-US-00013 TABLE 4 MMV value of different periods MMV (%) Time Comparative Comparative Comparative Comparative Comparative Example Example Example (min) Example 1 Example 2 Example 3 Example 4 Example 5 4 5 6 0 0 0 0 0 0 0 0 0 30 98.19 81.15 136.20 48.69 122.76 127.66 121.36 135.81 60 61.62 49.80 82.05 24.04 92.14 98.67 94.44 113.37 120 70.11 47.42 74.96 34.80 85.43 88.71 86.36 105.76

[0099] 2. Method for Testing Transepidermal Water Loss (TEWL)

[0100] 2.1 Principle of the Test

[0101] The measurement principle of the test instrument was derived from the Fick's law of diffusion: dm/dt=D.A.dp/dx. Two groups of temperature and humidity sensors were used to measure the water vapor pressure gradient formed by the transepidermal water loss at different testing regions near the epidermis (within 1 cm), so that the amount of water emitted from the percutaneous skin was directly measured. TEWL value is an important indicator of the skin barrier, and the lower the TEWL value of the skin is, the better the barrier function of the skin, and vice versa.

[0102] 2.2 Requirements for Measurement Conditions and Volunteers

[0103] Conditions of measurement environment: environment temperature of 221 C., humidity of 505%, and real-time dynamic detection.

[0104] Requirements for the volunteers: at least 30 valid volunteers, 1665 years old (excluding pregnant and lactating women); the baseline of the skin moisture content in the testing region on forearm measured by capacitance method was between 15 and 100; without serious systemic diseases, immunodeficiency or autoimmune diseases; without active allergic diseases; without history of severe allergies to skin care cosmetics; without using hormonal drugs and immunosuppressive agents in the past month. In addition, the volunteers had not participated in other clinical trials during the test period and used the test samples according to regulations, and the information was complete. All volunteers have complete data and signed the informed consent form before the test.

[0105] 1.4 Preparations Before the Test

[0106] 23 days before the test, no product (cosmetics or external drugs) was allowed to apply on the testing region. Before the test, the inner sides of forearms of the subjects were cleaned and wiped with a dry facial tissue. After cleaning, the test regions were marked on the inner side of forearm. Before the measurement, the subjects sit in a room which met the requirement standard for at least 30 minutes without drinking water. Their forearms were exposed as in the test state, and kept relaxed.

[0107] 2.4 Processes of Measurement

[0108] In the test, a testing region of 33 cm.sup.2 was marked on the inner side of the left and right arms, and multiple regions might be marked on the same arm with an interval of 1 cm. The test samples and the blank control were randomly distributed on the left and right arms. Capacitive skin tester was used to perform the measurement on the testing regions and the control regions. Each area was measured 15 times in parallel. The basal value of each testing region was measured first, and then the test sample was evenly applied to the testing region with a latex finger sleeve at a dosage of 2.00.1 mg sample/cm.sup.2. Moisture contents of skin in the testing region and the blank control region were measured after 1 hour, 2 hours and 4 hours (according to this time at the time of verification). The measurement of the same volunteer was performed by the same surveyor.

[0109] Analysis of Data

[0110] TEWL values of different periods were respectively measured according to the design of the test.

[0111] Formula of Calculation:

T %=(T1T0)/T0100%

[0112] Comment: T %=reducing percentage of skin transepidermal water loss (TEWL) [0113] T0=moisture losing value of skin before using the sample [0114] T1=moisture losing value of skin after using the sample

[0115] The results were shown in Table 5 and FIG. 2.

TABLE-US-00014 TABLE 5 TWEL values of different periods TEWL (%) Time Comparative Comparative Comparative Comparative Comparative Example Example Example (min) Example 1 Example 2 Example 3 Example 4 Example 5 4 5 6 0 0 0 0 0 0 0 0 0 30 7.80 4.77 0.69 3.07 1.08 10.77 11.48 12.41 60 12.90 8.80 0.98 2.17 9.99 11.81 11.79 13.47 120 13.36 7.83 0.89 5.56 10.63 13.96 14.56 17.92

[0116] In combination of FIGS. 12 and tables 45, it could be concluded that the composition provided by the present disclosure has a scientific formulation, which can increase the real-time moisture content of skin by 135.81%, and maintain the moisture content of skin by an increase of 105.76% at 2 h. In addition, the composition shows an ability of greatly lowering skin moisture loss (TEWL), which helps the skin to be fully moisturized. Comparing with comparative examples 15, the formulas of examples 46 have significant effects (p<0.05) on increasing moisture content and inhibiting moisture loss, indicating that the combination of the ingredients has a notably synergistic effect.

[0117] The above are merely preferred embodiments of the present disclosure. It should be noted that one of ordinary skill in the art can also make several improvements and refinements without departing from the principles of the present disclosure. These improvements and refinements should also be regarded as the scope of protection of the present disclosure.