BIOMASS FORMULATION

Abstract

The present invention relates to a solid formulation comprising a carotenoid producing source bioorganism, which is storage stable, as well as the process of production of this formulation.

Claims

1. A process for the preparation of the solid formulation according to any of the preceding claims comprising the following steps in any order: (a) optionally pasteurizing the harvested fermentation broth; (b) harvesting the bioorganism from the fermentation broth and re-suspending the bioorganism pellet in an aqueous medium (c) discarding the supernatant; (d) optionally resuspending the bioorganism pellet in an aqueous medium and again harvesting the bioorganism (e) optionally rupturing the bioorganisms (e.g. by milling); (f) optionally adding at least one antioxidant; (g) adding at least one hydrocolloid as well as adding optionally at least one auxiliary; (h) drying the solution.

2. The process according to claim 1, wherein step (b) comprises centrifuging the fermentation broth to harvest the bioorganism.

3. The process according to claim 1, wherein optional step (e) comprise milling the bioorganisms.

4. The process according to claim 1, wherein the dried solution resulting from step (h) comprises a solid formulation comprising (i) 25 to 75 wt-%, based on the total weight of the solid formulation, of at least one source bioorganism producing carotenoid(s), retinolic compound(s) or other small molecule lipophilic agent(s), and (ii) 25 to 75 wt-%, based on the total weight of the solid formulation, of at least one hydrocolloid, and (iii) optionally 0.1 to 10 wt-%, based on the total weight of the solid formulation, of at least one antioxidant and (iv) optionally 5 to 20 wt-%, based on the total weight of the solid formulation, of at least one auxiliary agent.

5. The process according to claim 4, wherein the source bioorganism is chosen from the group consisting of animal bioorganism, mammalian bioorganism, insect bioorganism, plant bioorganism, fungal bioorganism, yeast bioorganism, algal bioorganism, bacterial bioorganism, cyanobacterial bioorganism, archaebacterial bioorganism and protozoal bioorganisms.

6. The process according to claim according to claim 4, wherein the source bioorganism is producing a carotenoid which is chosen from the group consisting of antheraxanthin, adonirubin, adonixanthin, astaxanthin, canthaxanthin, capsorubrin, -cryptoxanthin, -carotene, -carotene, ,-carotene, -carotene, -carotene, echinenone, 3-hydroxyechinenone, 3-hydroxyechinenone, -carotene, -carotene, 4-keto--carotene, -carotene, -cryptoxanthin, deoxyflexixanthin, diatoxanthin, 7,8-didehydroastaxanthin, didehydrolycopene, fucoxanthin, fucoxanthinol, isorenieratene, -isorenieratene, lactucaxanthin, lutein, lycopene, mimulaxanthin, myxobactone, neoxanthin, neurosporene, hydroxyneurosporene, peridinin, phytoene, rhodopin, rhodopin glucoside, rhodoxanthin, 4-keto-rubixanthin, siphonaxanthin, spheroidene, spheroidenone, spirilloxanthin, torulene, 4-keto-torulene, 3-hydroxy-4-keto-torulene, uriolide, uriolide acetate, violaxanthin, zeaxanthin--diglucoside, zeaxanthin, and C.sub.30 carotenoids; as well as derivatives thereof.

7. The process according to claim according to claim 4, wherein the hydrocolloid may be chosen from, but is not limited to the group consisting of agar-agar, carrageenan, gelatin, pectin, xanthan gum, gum arabic, guar gum, locust bean gum, cellulose derivatives as carboxymethyl cellulose, alginate, lignosulfonate, plant proteins (such as soy protein isolate or lupin protein isolates) and starch (also modified starches such as OSA-modified starches.

8. The process according to claim according to claim 4, wherein the formulation comprised 0.1 to 10 wt.-%, based on the total weight of the solid formulation, of at least one antioxidants, chosen from the group including, but not limited to tocopherols, vitamin C, ethoxyquin, vitamin E, BHT, BHA and TBHQ.

9. The process according to claim according to claim 4, wherein the formulation is 5 wt-% to 20 wt-%, based on the total weight of the solid formulation, of at least one auxiliary agent, which useful for the application in the food or feed product by improving the property of these compositions, physical stability, storage stability, visual perception, controlled release in the GI-tract, pH control, etc.

10. The process according to claim according to claim 4, wherein the total content of carotenoid(s), retinolic compound(s) or other small molecule lipophilic agent(s) is up to 20 wt-%, based on the total weight of the solid formulation.

11. The process according to claim according to claim 4, wherein the solid formulation comprises 0.1 to 10 wt-%, based on the total weight of the solid formulation, of at least one antioxidant.

Description

EXAMPLES

[0077] The following examples have been prepared as described in the description.

Example 1

[0078] Before the drying step:

TABLE-US-00001 Ingredient Amount [g] Biomass containing zeaxanthin 502 (10.8% solids) Gelatin 31.2 Ascorbyl Palmitate 1.04 Tocopherol 2.5 Maltodextrin 12.6

[0079] After the drying step (the solid formulation):

TABLE-US-00002 Ingredient Amount [wt-%] Biomass containing zeaxanthin 53.39 Gelatin 30.72 Ascorbyl Palmitate 1.02 Tocopherol 2.46 Maltodextrin 12.41

Example 2

[0080] Before the drying step:

TABLE-US-00003 Ingredient Amount [g] Biomass containing zeaxanthin 500 (10.8% solids) Calcium Lignosulfonate 72 Ethoxyquin 3 Maltodextrin 12.0

[0081] After the drying step (the solid formulation):

TABLE-US-00004 Ingredient Amount [wt-%] Biomass containing zeaxanthin 38.3 Calcium Lignosulfonate 51.1 Ethoxyquin 2.1 Maltodextrin 8.5

Example 3

[0082] Before the drying step:

TABLE-US-00005 Ingredient Amount [g] Biomass containing acetylated zeaxanthin 525 (10.3% solids) Calcium Lignosulfonate 61 Ethoxyquin 2.55 Maltodextrin 10.2

[0083] After the drying step (the solid formulation)

TABLE-US-00006 Ingredient Amount [wt-%] Biomass containing acetylated zeaxanthin 42.32 Calcium Lignosulfonate 47.71 Ethoxyquin 1.99 Maltodextrin 7.98

Example 4

[0084] Before the drying step:

TABLE-US-00007 Ingredient Amount [g] Biomass containing zeaxanthin 1000 (10.8% solids) Calcium Lignosulfonate 145 Ethoxyquin 6 Maltodextrin 25

[0085] After the drying step (the solid formulation):

TABLE-US-00008 Ingredient Amount [g] Biomass containing zeaxanthin 38.0 Calcium Lignosulfonate 51.1 Ethoxyquin 2.1 Maltodextrin 8.8

Example 5

[0086] Before the drying step:

TABLE-US-00009 Ingredient Amount [g] Biomass containing acetylated zeaxanthin 1010 (10.3% solids) Calcium Lignosulfonate 145 Ethoxyquin 6.2 Maltodextrin 25

[0087] After the drying step (the solid formulation):

TABLE-US-00010 Ingredient Amount [wt-%] Biomass containing acetylated zeaxanthin 37.12 Calcium Lignosulfonate 51.74 Ethoxyquin 2.22 Maltodextrin 8.92

[0088] All of these formulations are stable as such as well as in a further formulated product. The loss of the carotenoid is less than 20% (after storage of 12 months at 25 C.).