DICHALCOGENIDE PRODRUGS
20240156965 ยท 2024-05-16
Assignee
Inventors
Cpc classification
C07D421/12
CHEMISTRY; METALLURGY
A61P29/00
HUMAN NECESSITIES
A61K45/06
HUMAN NECESSITIES
A61P9/10
HUMAN NECESSITIES
International classification
A61P35/00
HUMAN NECESSITIES
A61K45/06
HUMAN NECESSITIES
Abstract
The present application relates to a compound having the formula (I) A-L-B wherein A is represented by (AA), L is a bond or a self-immolative spacer; and B is represented by (BB) or (CC). The compound is capable of releasing molecular cargo in the presence of a reductase and is thus suitable for diagnosing or quantifying the activity of the reductase and for treating, ameliorating, preventing or diagnosing a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury.
##STR00001##
Claims
1. A compound having the formula (I)
A-L-B(I) wherein A is represented by ##STR00107## denotes the attachment point of A to L; L is a bond or a self-immolative spacer; B is represented by ##STR00108##
denotes the attachment point of B to L; A.sup.1 is selected such that A.sup.1-OH is a therapeutic, diagnostic or theranostic agent which contains an OH group that is attached to a 5- or 6-membered aromatic or heteroaromatic ring; A.sup.2 and A.sup.3 are independently selected such that A.sup.2-NH-A.sup.3 is a therapeutic, diagnostic or theranostic agent which contains an NH.sub.2 or NH moiety; K.sup.1 is selected from C.sub.1-4-alkyl optionally substituted by W.sup.1; K.sup.2 is selected from H, OR.sup.j, and OR.sup.k; or K.sup.1 and K.sup.2 are bonded together and K.sup.1 and K.sup.2 represent X.sup.3YX, wherein X.sup.3 is bonded to N and X is bonded to C; X is selected from N(R.sup.a), N(R.sup.b), CR.sup.c.sub.2 and O; X.sup.1 is (CR.sup.d.sub.2).sub.m; X.sup.2 is (CR.sup.e.sub.2).sub.n; X.sup.3 is CR.sup.f.sub.2; Y is (CR.sup.g.sub.2).sub.p; Z.sup.1 and Z.sup.2 are independently selected from S or Se such that either Z.sup.1 is Se and Z.sup.2 is S, or Z.sup.1 is S and Z.sup.2 is Se, or Z.sup.1 and Z.sup.2 are both Se; W is independently from OH, C(O)N(R.sup.h)(R.sup.i), N(R.sup.h)(R.sup.i), PR.sup.x.sub.3.sup.+, C(O)-4-(morpholine), C(O)-1-(piperazine), C(O)-1-(4-methylpiperazine), C(O)-1-(4-ethylpiperazine), and a heterocyclic group selected from azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, piperazin-1-yl, 4-methylpiperazin-1-yl, 4-ethylpiperazin-1-yl, 4-(2-hydroxyethyl)piperazin-1-yl, or morpholino, wherein that heterocyclic group is attached to the C.sub.1-4-alkyl or (C2-4-alkylene)- group via the N atom; W.sup.1 is selected from OH, C(O)N(R.sup.h)(R), N(R.sup.h)(R), PR.sup.x.sub.3.sup.+, C(O)-4-(morpholine), C(O)-1-(piperazine), C(O)-1-(4-methylpiperazine), C(O)-1-(4-ethylpiperazine), and a heterocyclic group selected from azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, piperazin-1-yl, 4-methylpiperazin-1-yl, 4-ethylpiperazin-1-yl, 4-(2-hydroxyethyl)piperazin-1-yl, or morpholino, wherein that heterocyclic group is attached to the C.sub.1-4-alkyl or (C2-4-alkylene)- group via the N atom; R.sup.a is selected from H, C.sub.1-4-alkyl, C(O)C.sub.1-4-alkyl, C(O)OC.sub.1-4-alkyl, C(O)N(C.sub.1-4-alkyl).sub.2, S(O).sub.2C.sub.1-4-alkyl, and (C.sub.2-4-alkylene)-O(C.sub.1-4-alkyl), wherein C.sub.1-4-alkyl or (C.sub.2-4-alkylene)- can be optionally substituted by W; R.sup.b is an acyl group of a monopeptide selected from -proteinogenic amino acids attached via a carboxy group; R.sup.c groups are independently selected from H and C.sub.1-4-alkyl; R.sup.d groups are independently selected from H and C.sub.1-4-alkyl; R.sup.e groups are independently selected from H and C.sub.1-4-alkyl; R.sup.f groups are independently selected from H and C.sub.1-4-alkyl; R.sup.9 groups are independently selected from H and C.sub.1-4-alkyl; R.sup.h is independently selected from H, C.sub.1-4-alkyl and CH.sub.2CH.sub.2OH; R.sup.i is independently selected from H, C.sub.1-4-alkyl and CH.sub.2CH.sub.2OH; R.sup.j is selected from H, C.sub.1-4-alkyl, C(O)C.sub.1-4-alkyl, C(O)OC.sub.1-4-alkyl, C(O)N(C.sub.1-4-alkyl).sub.2, S(O).sub.2C.sub.1-4-alkyl, and (C.sub.2-4-alkylene)-O(C.sub.1-4-alkyl), wherein C.sub.1-4-alkyl or (C.sub.2-4-alkylene)- can be optionally substituted by W.sup.1, R.sup.k is an acyl group of a monopeptide selected from -proteinogenic amino acids attached via a carboxy group; R.sup.x groups are independently selected from phenyl- and 4-methoxyphenyl-; m is 0, 1 or 2; n is 1 or 2, provided that m+n is 2 or 3; p is 0, 1, or 2, provided that when K.sup.1 and K.sup.2 are bonded together and K.sup.1 and K.sup.2 represent X.sup.3YX and X represents N(R.sup.a) or N(R.sup.b), then p=1 or 2; or any pharmaceutically acceptable salt, solvate or ester thereof.
2. The compound according to claim 1, wherein either Z.sup.1 is Se and Z.sup.2 is S, or Z.sup.1 is S and Z.sup.2 is Se.
3. The compound according to claim 1, wherein L is a bond.
4. The compound according to claim 1, wherein L is a self-immolative spacer selected from ##STR00109## denotes the attachment point to A;
denotes the attachment point to B; R is independently selected from halogen, O(R), N(R.sup.s)(R.sup.t), NO.sub.2, CN, and a heterocyclic group selected from azetidinyl, pyrrolidinyl, piperidinyl or morpholino, wherein the heterocyclic group is attached to the phenyl ring via the N atom; q is 0, 1, 2, 3 or 4; R.sup.r is independently selected from H, C.sub.1-4-alkyl and (C.sub.2-4-alkylene)-O(C.sub.1-4-alkyl), wherein C.sub.1-4-alkyl or (C.sub.2-4-alkylene)- can be optionally substituted by W; R.sup.s is independently selected from H, C(O)C.sub.1-4-alkyl and C.sub.1-4-alkyl; and R.sup.t is independently selected from H, C(O)C.sub.1-4-alkyl and C.sub.1-4-alkyl; preferably L is a self-immolative spacer selected from ##STR00110##
5. The compound according claim 1, wherein X.sup.1 and X.sup.2 are CH.sub.2.
6. The compound according to claim 1, wherein K.sup.1 and K.sup.2 are bonded together and K.sup.1 and K.sup.2 represent X.sup.3YX.
7. The compound according to claim 1, wherein K.sup.1 is C.sub.1-4-alkyl optionally substituted by W.sup.1 and K.sup.2 is H, OR.sup.j or OR.sup.k.
8. The compound according to claim 1, wherein A.sup.1-OH or A.sup.2-NH-A.sup.3 is selected from a diagnostically acceptable dye, a therapeutically acceptable DNA-alkylating agent, a therapeutically acceptable tubulin-inhibiting agent, and a therapeutically acceptable topoisomerase-inhibiting agent, or wherein A.sup.1-OH or A.sup.2-NH-A.sup.3 is selected from 10-hydroxycamptothecin, 10-hydroxybelotecan, 10-hydroxygimatecan, 10-hydroxy-CKD-602, 10-hydroxy-BNP-1350, 10-hydroxy-sinotecan, topotecan, 7-ethyl-10-hydroxy-camptothecin (SN-38), 10-hydroxy-20-acetoxy-camptothecin, pyrrolobenzodiazepine, methotrexate, duocarmycin, CC-1065, doxorubicin, epirubicin, daunorubicin, pirarubicin, carminomycin, doxorubicin-N,O-acetal, 4-(bis(2-chloroethyl)amino)phenol, 4-(bis(2-bromoethyl)amino)phenol, 4-(bis(2-mesylethyl)amino)phenol, 4-((2-chloroethyl-2-mesylethyl)amino)phenol, 5-hydroxy-seco-cyclopropabenzaindoles, 5-hydroxy-seco-(2-methyl-cyclopropa)benzaindoles, 5-hydroxy-seco-cyclopropamethoxybenzaindoles, 5-amino-seco-cyclopropabenzaindoles, etoposide, teniposide, GL331, NPF, TOP53, NK611, tubulysin A, tubulysin B, tubulysin C, tubulysin G, tubulysin I, monomethyl auristatin E, monomethyl auristatin F, dolastatin 10, dolastatin 15, symplostastin 1, symplostastin 3, narciclasine, pancratistatin, 2-epi-narciclasine, narciprimine, calicheamicin ?1, calicheamicin ?1, calicheamicin ?1, calicheamicin ?1, calicheamicin F, calicheamicin ?, calicheamicin T, diclofenac, aceclofenac, mefenamic acid, clonixin, piroxicam, meloxicam, tenoxicam, lornoxicam, baricitinib, filgotinib, tofacitinib, upadacitinib, ruxolitinib, peficitinib, decemotinib, solcitinib, itacitinib, fostamatinib, SHR0302, leuco-methylene blue, leuco-methyl methylene blue, leuco-dimethyl methylene blue, leuco-toluidine blue, leuco-Azure A, leuco-Azure B, leuco-Azure C, leuco-Thionin, leuco-methylene violet, leuco-new methylene blue, leuco-Nile blue A, leuco-brilliant cresyl blue, firefly luciferin (D-Luciferin), umbelliferone, 4-trifluoromethylumbelliferone, 6,8-difluoro-4-methylumbelliferone, 7-hydroxycoumarin-3-carboxylic acid, 6,8-difluoro-7-hydroxy-5-methylcoumarin (DiFMU), 7-amino-4-methylcoumarin, 7-amino-4-chloromethylcoumarin, 3-O-methylfluorescein, 3-O-ethyl-5-carboxyfluorescein, 2,7-difluoro-3-O-methylfluorescein, 3-N-acetyl-rhodamine, 3-N-acetyl-dimethylsilarhodamine, 2,7-dibromo-3-N-acetyl-dimethylcarborhodamine, 3-N-acetyl-6-carboxyrhodamine, 2,7-difluoro-3-N-acetylrhodol, 3-O(N,N-dimethyl-2-aminoethyl)-6-carboxyfluorescein, 2,7-dichloro-3-O(N,N-dimethyl-2-amino-ethyl)fluorescein, blackberry quencher (BBQ), black hole quencher 3 (BHQ3), 2-(2-hydroxyphenyl)quinazolin-4-one, 6-chloro-2-(5-chloro-2-hydroxyphenyl)quinazolin-4-one, and 6-bromo-2-(5-bromo-2-hydroxyphenyl)quinazolin-4-one.
9. A pharmaceutical or diagnostic composition comprising the compound according to claim 1, or a pharmaceutically acceptable salt, solvate, or ester thereof, and optionally a pharmaceutically acceptable carrier or excipient.
10. The pharmaceutical or diagnostic composition according to claim 9, further comprising a second pharmaceutically active agent selected from a vascular disrupting agent, a cytotoxic chemotherapeutic agent and an immunomodulator.
11. The pharmaceutical or diagnostic composition according to claim 10, wherein the second pharmaceutically active agent is selected from combretastatin A-4 (CA4), 3-aminocombretastatin A-4, BNC105, ABT-751, ZD6126, combretastatin A-1, or prodrugs of the same (which includes but is not limited to combretastatin A-4 phosphate (CA4P), 3-aminocombretastatin A-4 3-serinamide (ombrabulin), combretastatin A-1 bisphosphate (CA1P), and BNC105 phosphate (BNC105P)), and pharmaceutically acceptable salts, solvates or esters of the same.
12. (canceled)
13. A method of treating, ameliorating, preventing or diagnosing a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury, wherein preferably the neoplastic disorder is cancer which is preferably selected from acoustic neuroma, adenocarcinoma, angiosarcoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, breast cancer, bronchogenic carcinoma, cervical cancer, chondrosarcoma, chordoma, choriocarcinoma, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliosarcoma, ependymoma, epithelial carcinoma, Ewing's tumor, fibrosarcoma, hemangioblastoma, leiomyosarcoma, liposarcoma, Merkel cell carcinoma, melanoma, mesothelioma, myelodysplastic syndrome, myxosarcoma, oligodendroglioma, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinomas, papillary carcinoma, pinealoma, prostate cancer, renal cell carcinoma, retinoblastoma, rhabdomyosarcoma, sebaceous gland carcinoma, seminoma, squamous cell carcinoma, sweat gland carcinoma, synovioma, testicular tumor, Wilms' tumor, adrenocortical carcinoma, urothelial carcinoma, gallbladder cancer, parathyroid cancer, Kaposi sarcoma, colon carcinoma, gastrointestinal stromal tumor, anal cancer, rectal cancer, small intestine cancer, brain tumor, glioma, glioblastoma, astrocytoma, neuroblastoma, medullary carcinoma, medulloblastoma, meningioma, leukemias including acute myeloid leukemia, multiple myeloma, acute lymphoblastic leukemia, liver cancer including hepatoma and hepatocellular carcinoma, lung carcinoma, non-small-cell lung cancer, small cell lung carcinoma, lymphangioendotheliosarcoma, lymphangiosarcoma, primary CNS lymphoma, non-Hodgkin lymphoma, and classical Hodgkin's lymphoma, preferably colon cancer, rectal cancer, small intestine cancer, brain tumor, leukemia, liver cancer, lung cancer, lymphoma, basal cell carcinoma, breast cancer, cervical cancer, melanoma, ovarian cancer, pancreatic cancer, and squamous cell carcinoma, wherein an effective amount of a compound having the formula (I) as defined in claim 1, or a pharmaceutically acceptable salt, solvate, or ester thereof, is administered to a patient in need thereof.
14. A method of predicting the suitability of a compound having the formula (I) as defined in claim 1, or a pharmaceutically acceptable salt, solvate, or ester thereof, for treating a patient who is suffering from a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury, wherein the method comprises: (i) obtaining a sample from the patient; (ii) contacting the sample with a compound having the formula (I) as defined in any claim 1, or a pharmaceutically acceptable salt, solvate, or ester thereof, wherein A.sup.1 is selected such that A.sup.1-OH is a diagnostic or theranostic agent or A.sup.2 and A.sup.3 are independently selected such that A.sup.2-NH-A.sup.3 is a diagnostic or theranostic agent; and (iii) detecting the presence or absence of A.sup.1-OH or A.sup.2-NH-A.sup.3.
15. A method of determining an inhibitory activity of a candidate inhibitor or candidate drug upon an oxidoreductase and/or a redox effector protein, wherein the method comprises: (i) contacting a compound having the formula (I) as defined in claim 1, or a pharmaceutically acceptable salt, solvate, or ester thereof, wherein A.sup.1 is selected such that A.sup.1-OH is a diagnostic or theranostic agent or A.sup.2 and A.sup.3 are independently selected such that A.sup.2-NH-A.sup.3 is a diagnostic or theranostic agent, with the oxidoreductase and/or the redox effector protein as well as the candidate inhibitor or candidate drug; and (ii) detecting the presence or absence of A.sup.1-OH or A.sup.2-NH-A.sup.3.
Description
DESCRIPTION OF THE FIGURES
[0093]
[0094]
[0095]
[0096]
[0097]
DEFINITIONS
[0098] Unless stated otherwise the following definitions apply.
[0099] The term alkyl refers to a saturated straight or branched hydrocarbon chain. In any of the below definitions, C.sub.1-4-alkyl is not particularly limited, and refers to a saturated straight or branched hydrocarbon chain, which has 1 to 4 carbon atoms, preferably 1 to 3 carbon atoms, more preferably 1 or 2 carbon atoms, even more preferably 1 carbon atom. Preferably C.sub.1-4-alkyl can be methyl, ethyl, n-propyl, or i-propyl, more preferably it can be methyl or ethyl, most preferably it can be methyl.
[0100] In any of the below definitions, C.sub.2-4-alkylene is not particularly limited, and refers to a saturated straight or branched hydrocarbon chain, which has 2 to 4 carbon atoms, preferably 2 or 3 carbon atoms, more preferably 2 carbon atoms. Preferably C.sub.2-4-alkylene can be ethylene, or n-propylene, more preferably it can be ethylene.
[0101] An aromatic ring is any not particularly limited and refers to any aromatic carbocyclic ring, which has 5 to 7 carbon atoms, preferably 5 or 6 carbon atoms, more preferably the aromatic ring is phenyl. The aromatic ring can optionally be annulated to one or more carbocyclic or heterocyclic rings. Examples of annulated rings include but are not limited to naphthyl, quinolyl, isoquinolyl, quinoxalyl, benzopyranonyl, benzo[1,3]dioxolyl, benzothiazolyl, 2,3-dihydro-1H-benzo[e]indole, xanthenyl or indolyl, preferably naphthyl, quinolyl or xanthenyl.
[0102] The heteroaromatic ring is any not particularly limited and refers to any aromatic heterocyclic ring, which has 5 to 7 ring atoms, preferably 5 or 6 ring atoms. The heteroaromatic ring can optionally be annulated to one more carbocyclic or heterocyclic rings. Examples of the heteroaromatic ring include thiophenyl, pyridinyl, pyrazolyl, pyrimidinyl, purinyl, pyrrolo, furanyl, oxazolyl, thiazolyl, imidazolyl, benzofuranyl, benzothiophenyl, benzothiazolyl, benzoxazolyl or naphthyridinyl, preferably pyrrolo, pyridinyl, thiophenyl or furanyl.
[0103] An aliphatic moiety is a saturated or unsaturated, linear, branched or cyclic moiety containing between 1 to 50 carbon atoms and optionally 0 to 19 heteroatoms, preferably 0 to 15 heteroatoms, wherein the heteroatoms are typically chosen from O, N, S, Se, Si, Hal, B or P, preferably chosen from O, N, Hal, S, Si or P, more preferably O, N, Hal, S, Si, even more preferably chosen from O, N or Hal.
[0104] A carbocyclic ring is a cyclic structure containing from 4 to 50 carbon atoms. Examples of the carbocyclic ring include cyclobutane, cyclopentane, cyclohexane, benzene, cycloheptane, naphthalene or anthracene, preferably cyclohexane, cyclopentane, benzene or naphthalene, more preferably benzene or naphthalene.
[0105] A heterocyclic ring is a cyclic structure containing at least one heteroatom selected from N, O, Si, S, B, P, and Se, and containing at least one carbon atom. Examples of the heterocyclic ring include thiophene, pyridine, pyrazole, pyrimidine, pyrrole, furan, oxazole, thiazole, imidazole, quinoline, isoquinoline, benzofuran, benzothiophene, benzothiazole, benzoxazole, naphthyridine, piperidine, piperazine, pyrrolidine, tetrahydrothiophene, tetrahydrofuran or pyran, preferably pyridine, furan, thioazole, quinoline, isoquinoline, benzothiazole, piperidine, piperazine or pyran, more preferably pyridine, quinoline, piperidine or piperazine.
[0106] Halogen refers to F, Cl, Br or I, preferably F or Cl.
[0107] If a moiety is referred to as being optionally substituted by a substituent it can in each instance include one or more of the indicated substituents.
[0108] Oxidoreductase (also referred to as disulfide reductase or disulfide oxidoreductase) refers to an enzyme whose biochemical reactivity includes reducing disulfides to the corresponding thiols. Examples thereof include thioredoxin reductase 1 (TrxR1), TrxR2, thioredoxin glutathione reductase (TrxR3/TGR), methionine-R-sulfoxide reductase (MsrB1), or glutathione disulfide reductase (GR). A disulfide effector protein (or redox effector protein or simply effector protein) is a redox-active protein, typically with a dithiol active site such as a thioredoxin-like domain but alternatively also with a monothiol or selenol active site, that is reduced directly or indirectly by an upstream catalytic disulfide oxidoreductase and fulfils redox-active functions. Examples thereof include thioredoxins (Trxs) such as Trx1 and Trx2; thioredoxin-related proteins such as thioredoxin-related protein of 14 kDa (TRP14) and thioredoxin-related transmembrane proteins (TMXs) such as TMX1, TMX2, TMX3, and TMX4; thioredoxin-like protein 1 (TXNL1); human macrothioredoxin (hMTHr); glutaredoxins such as Grx1, Grx2, Grx3, Grx4, Grx5, GrxA, and Grx6; glutathione peroxidases (GPxs) such as GPx1, GPx4, GPx2; protein disulfide isomerases (PDIs) such as PDIA1, PDIA2, PDIA3, PDIA4, PDIA5, PDIA6, EndoPDI, PDIp, endoplasmic reticulum resident protein 18 (ERp18), ERp44, ERp57, ERp72, ERdj5, disulfide bond formation protein A (DsbA), DsbB, DsbC.
[0109] The term cancer, as used herein, refers to a group of proliferative diseases characterized by uncontrolled division of abnormal cells in a subject. Non-limiting examples of cancers include acoustic neuroma, adenocarcinoma, angiosarcoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, breast cancer, bronchogenic carcinoma, cervical cancer, chondrosarcoma, chordoma, choriocarcinoma, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliosarcoma, ependymoma, epithelial carcinoma, Ewing's tumor, fibrosarcoma, hemangioblastoma, leiomyosarcoma, liposarcoma, Merkel cell carcinoma, melanoma, mesothelioma, myelodysplastic syndrome, myxosarcoma, oligodendroglioma, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinomas, papillary carcinoma, pinealoma, prostate cancer, renal cell carcinoma, retinoblastoma, rhabdomyosarcoma, sebaceous gland carcinoma, seminoma, squamous cell carcinoma, sweat gland carcinoma, synovioma, testicular tumor, Wilms' tumor, adrenocortical carcinoma, urothelial carcinoma, gallbladder cancer, parathyroid cancer, Kaposi sarcoma, colon carcinoma, gastrointestinal stromal tumor, anal cancer, rectal cancer, small intestine cancer, brain tumor, glioma, glioblastoma, astrocytoma, neuroblastoma, medullary carcinoma, medulloblastoma, meningioma, leukemias including acute myeloid leukemia, multiple myeloma, acute lymphoblastic leukemia, liver cancer including hepatoma and hepatocellular carcinoma, lung carcinoma, non-small-cell lung cancer, small cell lung carcinoma, lymphangioendotheliosarcoma, lymphangiosarcoma, primary CNS lymphoma, non-Hodgkin lymphoma, and classical Hodgkin's lymphoma.
[0110] The term cargo, as used herein, represents a therapeutic, diagnostic or theranostic agent to be delivered to a site of enzymatic activity or disease (e.g. a tumour, a site of inflammatory or autoimmune disease). Cargos used in the compounds of the invention may be small molecules. Examples include agents for treating, ameliorating, preventing or diagnosing a neoplastic disorder, an inflammatory disorder (e.g. a non-steroidal anti-inflammatory drug (NSAID), a disease-modifying anti-rheumatic drug (DMARD), or mesalamine), atherosclerosis; an autoimmune disorder; a chronic inflammatory autoimmune disorder; ischaemia; and reperfusion injury as well as kinase inhibitors. Examples also include diagnostically acceptable dyes, therapeutically acceptable DNA-alkylating agents, therapeutically acceptable topoisomerase-inhibiting agents or therapeutically acceptable tubulin-inhibiting agents.
[0111] Non-limiting examples of antineoplastic agents include 10-hydroxycamptothecin, 10-hydroxybelotecan, 10-hydroxygimatecan, 10-hydroxy-CKD-602, 10-hydroxy-BNP-1350, 10-hydroxy-sinotecan, topotecan, 7-ethyl-10-hydroxy-camptothecin (SN-38), 10-hydroxy-20-acetoxy-camptothecin, pyrrolobenzodiazepine, methotrexate, duocarmycin, CC-1065, doxorubicin, epirubicin, daunorubicin, pirarubicin, carminomycin, doxorubicin-N,O-acetal, 4-(bis(2-chloroethyl)amino)phenol, 4-(bis(2-bromoethyl)amino)phenol, 4-(bis(2-mesylethyl)amino)phenol, 4-((2-chloroethyl-2-mesylethyl)amino)phenol, 5-hydroxy-seco-cyclopropabenzaindoles, 5-hydroxy-seco-(2-methyl-cyclopropa)benzaindoles, 5-hydroxy-seco-cyclopropamethoxybenzaindoles, 5-amino-seco-cyclopropabenzaindoles, etoposide, teniposide, GL331, NPF, TOP53, NK611, tubulysin A, tubulysin B, tubulysin C, tubulysin G, tubulysin I, monomethyl auristatin E, monomethyl auristatin F, dolastatin 10, dolastatin 15, symplostastin 1, symplostastin 3, narciclasine, pancratistatin, 2-epi-narciclasine, narciprimine, calicheamicin ?1, calicheamicin ?1, calicheamicin ?1, calicheamicin ?1, calicheamicin ?, calicheamicin ?, or calicheamicin T.
[0112] Non-limiting examples of NSAIDs include diclofenac, aceclofenac, mefenamic acid, clonixin, piroxicam, meloxicam, tenoxicam, or lornoxicam.
[0113] Non-limiting examples of DMARDs include methotrexate.
[0114] Non-limiting examples of kinase inhibitors include baricitinib, filgotinib, tofacitinib, upadacitinib, ruxolitinib, peficitinib, decemotinib, solcitinib, itacitinib, fostamatinib, or SHR0302.
[0115] Particularly of interest are inhibitors of the Janus Kinase (JAK) family of kinases, such as of JAK1 or JAK3.
[0116] Cargos used in the compounds of the invention may be diagnostic agents (e.g., a fluorescent agent or a radiotracer agent), that may be used to detect and/or determine the stage of a targeted pathology such as a tumor, or to measure or localise redox activity. Non-limiting examples of fluorescent diagnostic agents include leuco-methylene blue, leuco-methyl methylene blue, leuco-dimethyl methylene blue, leuco toluidine blue, leuco-Azure A, leuco-Azure B, leuco-Azure C, leuco-Thionin, leuco-methylene violet, leuco-new methylene blue, leuco-Nile blue A, leuco-brilliant cresyl blue, firefly luciferin (D-Luciferin), umbelliferone, 4-trifluoromethylumbelliferone, 6,8-difluoro-4-methylumbelliferone, 7-hydroxycoumarin-3-carboxylic acid, 6,8-difluoro-7-hydroxy-5-methylcoumarin (DiFMU), 7-amino-4-methylcoumarin, 7-amino-4-chloromethylcoumarin, 3-O-methylfluorescein, 3-O-ethyl-5-carboxyfluorescein, 2,7-difluoro-3-O-methylfluorescein, 3-N-acetyl-rhodamine, 3-N-acetyl-dimethylsilarhodamine, 2,7-dibromo-3-N-acetyl-dimethylcarborhodamine, 3-N-acetyl-6-carboxyrhodamine, 2,7-difluoro-3-N-acetylrhodol, 3-O(N,N-dimethyl-2-aminoethyl)-6-carboxyfluorescein, 2,7-dichloro-3-O(N,N-dimethyl-2-aminoethyl)fluorescein, blackberry quencher (BBQ), black hole quencher 3 (BHQ3), 2-(2-hydroxyphenyl)quinazolin-4-one, 6-chloro-2-(5-chloro-2-hydroxyphenyl)quinazolin-4-one, or 6-bromo-2-(5-bromo-2-hydroxy-phenyl)quinazolin-4-one.
[0117] Radiotracer agents are agents useful for imaging which incorporate a radioactive isotope, non-limiting examples of which are radioactive isotopes of carbon, cobalt, fluorine, gallium, iodine, or technetium, preferably carbon, fluorine or iodine. Compounds of the present invention which have been labelled with a radioactive isotope can be employed as diagnostic applications.
[0118] The term pharmaceutically acceptable salt refers to a salt of a compound of the present invention. Suitable pharmaceutically acceptable salts include acid addition salts which may, for example, be formed by mixing a solution of compounds of the present invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulfuric acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid. Furthermore, where the compound carries an acidic moiety, suitable pharmaceutically acceptable salts thereof may include alkali metal salts (e.g., sodium or potassium salts); alkaline earth metal salts (e.g., calcium or magnesium salts); and salts formed with suitable organic ligands (e.g., ammonium, quaternary ammonium and amine cations formed using counteranions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl sulfonate and aryl sulfonate). Illustrative examples of pharmaceutically acceptable salts include, but are not limited to, acetate, adipate, ascorbate, aspartate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, butyrate, calcium edetate, camphorate, camphorsulfonate, camsylate, carbonate, chloride, citrate, clavulanate, cyclopentanepropionate, digluconate, dihydrochloride, dodecylsulfate, edetate, edisylate, estolate, esylate, ethanesulfonate, formate, fumarate, gluceptate, glucoheptonate, gluconate, glutamate, glycerophosphate, hemisulfate, heptanoate, hexanoate, hydrabamine, hydrobromide, hydrochloride, hydroiodide, 2-hydroxy-ethanesulfonate, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, lauryl sulfate, malate, maleate, malonate, mandelate, mesylate, methanesulfonate, methylsulfate, mucate, 2-naphthalenesulfonate, napsylate, nicotinate, nitrate, N-methylglucamine ammonium salt, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, pectinate, persulfate, 3-phenylpropionate, phosphate/diphosphate, picrate, pivalate, polygalacturonate, propionate, salicylate, stearate, sulfate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide, undecanoate, valerate, and the like (see, for example, S. M. Berge et al.; J. Pharm. Sci. 1977, 66, 1-19).
[0119] When the compounds of the present invention are provided in crystalline form, the structure can contain solvent molecules. The solvents are typically pharmaceutically acceptable solvents and include, among others, water (hydrates) or organic solvents. Examples of possible solvates include hydrates, ethanolates and iso-propanolates.
[0120] The term pharmaceutically acceptable ester refers to an ester of a compound of the present invention. Suitable pharmaceutically acceptable esters include acetyl, butyryl, and acetoxymethyl esters.
[0121] The term protecting group, as used herein (represented in schemes as moieties -PG), represents a group intended to protect a hydroxy or an amino group from participating in one or more undesirable reactions during chemical synthesis. The term O-protecting group, as used herein, represents a group intended to protect a hydroxy or carbonyl group from participating in one or more undesirable reactions during chemical synthesis. The term N-protecting group, as used herein, represents a group intended to protect a nitrogen containing (e.g., an amino or hydrazine) group from participating in one or more undesirable reactions during chemical synthesis. Exemplary O- and N-protecting groups include, but are not limited to: alkanoyl, aryloyl, or carbamyl groups such as formyl, acetyl, propionyl, pivaloyl, tert-butylacetyl, 2-chloroacetyl, 2-bromoacetyl, trifluoroacetyl, trichloroacetyl, phthalyl, o-nitrophenoxyacetyl, ?-chlorobutyryl, benzoyl, 4-chlorobenzoyl, 4-bromobenzoyl, triiso-propylsilyloxymethyl, 4,4-dimethoxytrityl, isobutyryl, phenoxyacetyl, 4-isopropylphenoxy-acetyl, dimethylformamidino, and 4-nitrobenzoyl. Other 0-protecting groups include, but are not limited to: substituted alkyl, aryl, and aryl-alkyl ethers (e.g., trityl; methylthiomethyl; methoxymethyl; benzyloxymethyl; siloxymethyl; 2,2,2,-trichloroethoxymethyl; tetrahydropyranyl; tetrahydrofuranyl; ethoxyethyl; 1-[2-(trimethylsilyl)ethoxy]ethyl; 2-trimethylsilylethyl; tert-butyl ether; p-chlorophenyl, p-methoxyphenyl, p-nitrophenyl, benzyl, p-methoxybenzyl, and nitrobenzyl); silyl ethers (e.g., trimethylsilyl; triethylsilyl; triisopropylsilyl; dimethylisopropylsilyl; tert-butyldimethylsilyl; tert-butyldiphenylsilyl; tribenzylsilyl; triphenylsilyl; and diphenymethylsilyl); carbonates (e.g., methyl, methoxymethyl, 9-fluorenylmethyl; ethyl; 2,2,2-trichloroethyl; 2-(trimethylsilyl)ethyl; vinyl, allyl, nitrophenyl; benzyl; methoxybenzyl; 3,4-dimethoxybenzyl; and nitrobenzyl). Other N-protecting groups include, but are not limited to, chiral auxiliaries such as protected or unprotected, L- or D-amino acids such as alanine, leucine, phenylalanine, and the like; sulfonyl-containing groups such as benzenesulfonyl, p-toluenesulfonyl, and the like; carbamate forming groups such as benzyloxycarbonyl, p-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl, 3,4-dimethoxybenzyloxycarbonyl, 3,5-dimethoxybenzyl oxycarbonyl, 2,4-dimethoxybenzyloxycarbonyl, 4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimethoxybenzyloxycarbonyl, 1-(p-biphenylyl)-lmethylethoxycarbonyl, a,a-dimethyl-3,5-dimethoxybenzyloxycarbonyl, benzhydryloxy carbonyl, tbutyloxycarbonyl, diisopropylmethoxycarbonyl, isopropyl-oxycarbonyl, ethoxycarbonyl, methoxycarbonyl, allyloxycarbonyl, 2,2,2,-trichloroethoxy-carbonyl, phenoxycarbonyl, 4-nitrophenoxy carbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, phenylthiocarbonyl, and the like, aryl-alkyl groups such as benzyl, triphenylmethyl, benzyloxymethyl, and the like and silyl groups such as trimethylsilyl, and the like. Useful N-protecting groups are formyl, acetyl, benzoyl, pivaloyl, tert-butylacetyl, alanyl, phenylsulfonyl, benzyl, tert-butyloxycarbonyl (Boc), and benzyloxycarbonyl (Cbz).
[0122] The term proteinogenic amino acids herein comprises the 20 principal naturally-occurring L-amino acids (glycine, alanine, valine, leucine, isoleucine, phenylalanine, proline, methionine, serine, threonine, cysteine, tyrosine, tryptophan, asparagic acid, glutamic acid, asparagine, arginine, histidine, lysine, asparagine and glutamine) which are to be attached, as the monopeptide, via their carboxyl terminus to the specified amine nitrogen of the compound, creating amides. Preferably the proteinogenic amino acid is selected from leucine, serine and lysine.
[0123] The term self-immolative spacer, also called traceless linker or self-immolative linker, as used herein, represents a multivalent (e.g., divalent) group covalently linking a dichalcogenide-containing group which will be further defined below (as the moiety A), to a cargo B, in such a way that reductive cleavage of the dichalcogenide bond in the moiety A is followed by a sequence of reactions which result in the cleavage of a bond between the self-immolative linker group and the cargo B, thereby releasing the cargo. Illustrative examples thereof include:
##STR00009##
[0124] The stereochemistry at the ring atoms bridging the two rings of the bicyclic dichalcogenide structure of the compounds of formula (I) of the invention is not particularly limited; any absolute configuration of the two bridging ring tertiary carbon atoms is within the scope of the compounds of formula (I). Either carbon can be separately either R, or S, or a mixture of R and S in any proportions (including racemic and undefined proportions); therefore the dichalcogenide ring may be considered to be cis-fused to the carbamate-containing ring with any given stereochemistry, or trans-fused to the carbamate-containing ring with any given stereochemistry, or present as a mixture in any proportions of cis-fused and trans-fused with any given stereochemistries (including racemic and undefined proportions). Preferably, the dichalcogenide ring is either cis-fused or trans-fused.
[0125] The term vascular disrupting agent refers to a compound that reduces tumor blood flow upon administration. Such agents are typically microtubule-depolymerising agents, and are well represented in the literature (Gill, Pharmacology & Therapeutics 2019, 202, 18-31); as known to those skilled in the art, these include combretastatin A-4 (CA4), 3-aminocombretastatin A-4, BNC105, ABT-751, ZD6126, combretastatin A-1, and prodrugs of the same, including but not limited to combretastatin A-4 phosphate (CA4P), 3-aminocombretastatin A-4 3-serinamide (ombrabulin), combretastatin A-1 bisphosphate (CA1P), and BNC105 phosphate (BNC105P).
[0126] The term cytotoxic chemotherapeutic agent refers to compounds that have antineoplastic and/or antitumoral efficacy and which are typically useful in the treatment of cancer, optionally as part of combination therapies. Such agents include paclitaxel, docetaxel, nab-paclitaxel, epothilone, mertansine, irinotecan, etoposide, teniposide, mitoxantrone, doxorubicin, daunorubicin, epirubicin, cisplatin, carboplatin, oxaliplatin, melphalan, chlorambucil, busulfan, methotrexate, permetrexed, cyclophosphamide, 5-fluorouracil, capecitabine, gemcitabine, cytarabine, fludarabine, mercaptopurine, vinolrebine, vinblastine, vincristine, dolastatin, monomethyl auristatin E, monomethyl auristatin F, bleomycin, dacarbazine, pyrrolobenzodiazepine, temozolomide, carmustine, mitomycin and procarbazine.
[0127] In the context of the invention the term immunomodulator refers to therapeutically active compounds that interact with the immune system, which may for example be useful in the treatment of cancer or autoimmune diseases, optionally as part of combination therapies. Such agents include anti-PD-1 antibody such as pembrolizumab and nivolumab, anti-PD-L1 antibody such as avelumab, anti-CTLA4 antibody such as ipilimumab, cytokine agonists such as proleukin and interferon alfa-2a/interferon alfa-2b, mesalamine, baricitinib, filgotinib, tofacitinib, upadacitinib, ruxolitinib, peficitinib, decemotinib, solcitinib, itacitinib, fostamatinib, and SHR0302.
DETAILED DESCRIPTION OF THE INVENTION
[0128] Compound Having the Formula (I)
[0129] The present invention provides a compound having the formula (I)
A-L-B(I) [0130] A is represented by
##STR00010## [0131] denotes the attachment point from A to L. [0132] Z.sup.1 and Z.sup.2 are independently selected from S or Se such that either Z.sup.1 is Se and Z.sup.2 is S, or Z.sup.1 is S and Z.sup.2 is Se, or Z.sup.1 and Z.sup.2 are both Se. In a preferred embodiment, either Z.sup.1 is Se and Z.sup.2 is S, or Z.sup.1 is S and Z.sup.2 is Se. In another preferred embodiment, Z.sup.1 and Z.sup.2 are Se. More preferably, Z.sup.1 is Se and Z.sup.2 is S.
[0133] In a first embodiment, K.sup.1 is selected from C.sub.1-4-alkyl optionally substituted by W.sup.1. Preferably when K.sup.1 is C.sub.1-4-alkyl optionally substituted by W.sup.1, K.sup.1 is -Me, -Et, (CH.sub.2).sub.2OH, (CH.sub.2).sub.2PPh.sub.3.sup.+, (CH.sub.2).sub.2C(O)-1-(4-methylpiperazine), (CH.sub.2).sub.2C(O)-morpholino, (CH.sub.2).sub.3C(O)-morpholino or (CH.sub.2).sub.3C(O)-1-(4-methylpiperazine), more preferably K.sup.1 is -Me, -Et, (CH.sub.2).sub.2C(O)-1-(4-methylpiperazine), or (CH.sub.2).sub.2C(O)-morpholino.
[0134] In the first embodiment, K.sup.2 is selected from H, OR.sup.j, and OR.sup.k; preferably K.sup.2 is selected from H, OMe, O(CH.sub.2).sub.2O(CH.sub.2).sub.2OH, O(CH.sub.2).sub.2PPh.sub.3.sup.+, or O(CH.sub.2).sub.3C(O)-1-(4-methylpiperazine); more preferably K.sup.2 is H, O(CH.sub.2).sub.2PPh.sub.3+, or O(CH.sub.2).sub.3C(O)-1-(4-methylpiperazine); more preferably K.sup.2 is H.
[0135] In a second embodiment, K.sup.1 and K.sup.2 are bonded together and K.sup.1K.sup.2 is X.sup.3YX, such that X.sup.3 is bonded to N and X is bonded to C. [0136] X is selected from N(R.sup.a), N(R.sup.b), CR.sup.c.sub.2 and O; preferably N(R.sup.a) or CR.sup.c.sub.2; more preferably CH.sub.2, NH, N(Me)-, N((CH.sub.2).sub.2OH), N((CH.sub.2).sub.2PPh.sub.3.sup.+), N((CH.sub.2).sub.3C(O)-1-(4-methylpiperazine))-. [0137] X.sup.1 is (CR.sup.d.sub.2).sub.m, preferably (CH.sub.2) or (CHMe)- or (CMe.sub.2)-, more preferably (CH.sub.2).
[0138] X.sup.2 is (CR.sup.e.sub.2).sub.n, preferably (CH.sub.2).
[0139] X.sup.3 is CR.sup.f.sub.2, preferably (CH.sub.2). [0140] Y is (CR.sup.g.sub.2).sub.p, preferably (CH.sub.2) or (CH.sub.2)(CH.sub.2), more preferably (CH.sub.2). [0141] W is selected from OH, C(O)N(R.sup.h)(R.sup.i), N(R.sup.h)(R.sup.i), PR.sup.x.sub.3.sup.+, C(O)-4-(morpholine), C(O)-1-(piperazine), C(O)-1-(4-methylpiperazine), C(O)-1-(4-ethylpiperazine), and a heterocyclic group. The heterocyclic group is selected from azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, piperazin-1-yl, 4-methylpiperazin-1-yl, 4-ethylpiperazin-1-yl, 4-(2-hydroxyethyl)piperazin-1-yl, or morpholino, wherein that heterocyclic group is attached to the C.sub.1-4-alkyl or (C.sub.2-4-alkylene)- group via the N atom. Preferably, W is selected from OH, C(O)N((CH.sub.2).sub.2OH).sub.2, N(CH.sub.3).sub.2, PPh.sub.3.sup.+, C(O)-1-(4-methylpiperazine), -azetidin-1-yl, or -morpholino. More preferably, W is selected from OH, N(CH.sub.3).sub.2, PPh.sub.3.sup.+, and C(O)-1-(4-methylpiperazine).
[0142] Disclosed is that W is selected from OH, C(O)N(R.sup.h)(R), N(R.sup.h)(R), PR.sup.x.sub.3.sup.+, -(morpholine-4-carbonyl), -(piperazine-1-carbonyl), -(4-methylpiperazine-1-carbonyl), -(4-ethylpiperazine-1-carbonyl), and a heterocyclic group selected from azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, piperazin-1-yl, 4-methylpiperazin-1-yl, 4-ethylpiperazin-1-yl, 4-(2-hydroxyethyl)piperazin-1-yl, or morpholino, wherein the heterocyclic group is attached to the C.sub.1-4-alkyl or (C.sub.2-4-alkylene)-group via the N atom. Preferably, W is selected from OH, C(O)N((CH.sub.2).sub.2OH).sub.2, N(CH.sub.3).sub.2, PPh.sub.3.sup.+, -(4-methylpiperazine-1-carbonyl), -azetidin-1-yl, or -morpholino. More preferably, W is selected from OH, N(CH.sub.3).sub.2, PPh.sub.3.sup.+, and -(4-methylpiperazine-1-carbonyl). [0143] W.sup.1 is selected from OH, C(O)N(R.sup.h)(R), N(R.sup.h)(R), PR.sup.x.sub.3.sup.+, C(O)-4-(morpholine), C(O)-1-(piperazine), C(O)-1-(4-methylpiperazine), C(O)-1-(4-ethylpiperazine), and a heterocyclic group. The heterocyclic group is selected from azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, piperazin-1-yl, 4-methylpiperazin-1-yl, 4-ethylpiperazin-1-yl, 4-(2-hydroxyethyl)piperazin-1-yl, or morpholino, wherein that heterocyclic group is attached to the C.sub.1-4-alkyl or (C.sub.2-4-alkylene)- group via the N atom. Preferably, W.sup.1 is selected from OH, C(O)N((CH.sub.2).sub.2OH).sub.2, N(CH.sub.3).sub.2, PPh.sub.3.sup.+, -(4-methylpiperazine-1-carbonyl), -azetidin-1-yl, or -morpholino. More preferably, W.sup.1 is selected from OH, N(CH.sub.3).sub.2, PPh.sub.3.sup.+, C(O)-1-(4-methylpiperazine), and C(O)-4-(morpholine).
[0144] Disclosed is that W.sup.1 is selected from OH, C(O)N(R.sup.h)(R), N(R.sup.h)(R), PR.sup.x.sub.3.sup.+, -(morpholine-4-carbonyl), -(piperazine-1-carbonyl), -(4-methylpiperazine-1-carbonyl), -(4-ethylpiperazine-1-carbonyl), and a heterocyclic group selected from azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, piperazin-1-yl, 4-methylpiperazin-1-yl, 4-ethylpiperazin-1-yl, 4-(2-hydroxyethyl)piperazin-1-yl, or morpholino, wherein the heterocyclic group is attached to the C.sub.1-4-alkyl or (C.sub.2-4-alkylene)-group via the N atom. Preferably, W.sup.1 is selected from OH, C(O)N((CH.sub.2).sub.2OH).sub.2, N(CH.sub.3).sub.2, PPh.sub.3.sup.+, -(4-methylpiperazine-1-carbonyl), -azetidin-1-yl, or -morpholino. More preferably, W.sup.1 is selected from OH, N(CH.sub.3).sub.2, PPh.sub.3.sup.+, and -(4-methylpiperazine-1-carbonyl). [0145] R.sup.a is selected from H, C.sub.1-4-alkyl, C(O)C.sub.1-4-alkyl, C(O)OC.sub.1-4-alkyl, C(O)N(C.sub.1-4-alkyl).sub.2, S(O).sub.2C.sub.1-4-alkyl, and (C.sub.2-4-alkylene)-O(C.sub.1-4-alkyl), wherein (C.sub.2-4-alkylene) or (C.sub.1-4-alkyl) can be optionally substituted by W. Preferably R.sup.a is selected from H and C.sub.1-4-alkyl, wherein (C.sub.1-4-alkyl) can be optionally substituted by W. More preferably, R.sup.a is selected from H, CH.sub.3, C.sub.2H.sub.5, and CH.sub.2CH.sub.2W. [0146] R.sup.b is an acyl group of a monopeptide selected from proteinogenic amino acids attached via a carboxy group. The attachment point is not particularly limited, the proteinogenic amino acid can be connected via the carboxy group at the ?-carbon or via a carboxy group of the side chain forming an amide group with the ring nitrogen atom, preferably the proteinogenic amino acid is connected via the carboxy group at the ?-carbon. The proteinogenic amino acid is not particularly limited and can be any amino acid as defined above; preferably leucine, serine or lysine; more preferably serine. In the case of serine, for example, R.sup.b is C(O)C(H)(NH.sub.2)CH.sub.2OH. [0147] R.sup.c groups are independently selected from H and C.sub.1-4-alkyl, preferably H. [0148] R.sup.d groups are independently selected from H and C.sub.1-4-alkyl, preferably H or Me. [0149] R.sup.e groups are independently selected from H and C.sub.1-4-alkyl, preferably H. [0150] R.sup.f groups are independently selected from H and C.sub.1-4-alkyl, preferably H. [0151] R.sup.9 groups are independently selected from H and C.sub.1-4-alkyl, preferably H. [0152] R.sup.h is selected from H, C.sub.1-4-alkyl and CH.sub.2CH.sub.2OH; preferably, from H, CH.sub.3 and CH.sub.2CH.sub.2OH. [0153] R.sup.i is selected from H, C.sub.1-4-alkyl and CH.sub.2CH.sub.2OH; preferably, from H, CH.sub.3 and CH.sub.2CH.sub.2OH. [0154] R.sup.j is selected from H, C.sub.1-4-alkyl, C(O)C.sub.1-4-alkyl, C(O)OC.sub.1-4-alkyl, C(O)N(C.sub.1-4-alkyl).sub.2, S(O).sub.2C.sub.1-4-alkyl, and (C.sub.2-4-alkylene)-O(C.sub.1-4-alkyl), wherein C.sub.1-4-alkyl or (C.sub.2-4-alkylene)- can be optionally substituted by W.sup.1. Preferably R.sup.j is selected from H and C.sub.1-4-alkyl, wherein (C.sub.1-4-alkyl) can be optionally substituted by W.sup.1. More preferably, R.sup.j is selected from H, CH.sub.3, C.sub.2H.sub.5, and CH.sub.2CH.sub.2W.sup.1. [0155] R.sup.k is an acyl group of a monopeptide selected from -proteinogenic amino acids attached via a carboxy group. The attachment point is not particularly limited, the proteinogenic amino acid can be connected via the carboxy group at the ?-carbon or via a carboxy group of the side chain forming an amide group with the ring nitrogen atom, preferably the proteinogenic amino acid is connected via the carboxy group at the ?-carbon. The proteinogenic amino acid is not particularly limited and can be any amino acid as defined above; preferably leucine, serine or lysine; more preferably serine. In the case of serine, for example, R.sup.k is C(O)C(H)(NH.sub.2)CH.sub.2OH. [0156] R.sup.x groups are independently selected from -phenyl and 4-methoxyphenyl-; [0157] m is 0, 1 or 2 and n is 1 or 2, provided that m+n is 2 or 3. Preferably m is 1 and n is 1. [0158] p is 0, 1, or 2, provided that when K.sup.1 and K.sup.2 are bonded together and represent X.sup.3YX, and X represents N(R.sup.a) or N(R.sup.b), then p=1 or 2. Preferably p is 1 or 2. More preferably p is 1. [0159] L is a bond or a self-immolative spacer. In one embodiment, L is a bond. In another embodiment, L is a self-immolative spacer. A self-immolative spacer is any linking group which is capable of covalently linking A and B and which can release the cargo H-B when the dichalcogenide bond in A is cleaved and the resulting dichalcogenol species cyclizes via nucleophilic attack at the carbonyl group of A. The self-immolative spacer is not particularly limited and can be chosen from any known self-immolative spacer. Examples are to be found in e.g. Blencowe, C. A. et al. Polym. Chem. 2011, 2, 773-790. https://doi.org/10.1039/C0PY00324G. Preferred examples thereof include, but are not limited to, the moieties shown in the Definitions section, and, in particular, para-hydroxybenzylic 1,4-elimination self-immolative spacers and para-hydroxybenzylic 1,6-elimination self-immolative spacers:
##STR00011##
[0160] In these formulae (R).sub.q can be attached at any available position on the benzene ring.
##STR00012##
can be attached at either of the carbon atoms which are indicated by the dashed lines.
[0161] More preferably the self-immolative spacer is a para-hydroxybenzylic 1,6-elimination spacer:
##STR00013##
[0162] In these formulae [0163] denotes the attachment from A to L. [0164]
denotes the attachment point from L to B.
[0165] If L is a bond, then the cargo B, which is a therapeutic, diagnostic or theranostic agent, can contain an OH group that is attached to a 5- or 6-membered aromatic or heteroaromatic ring, preferably an OH group that is attached to a 6-membered aromatic ring, more preferably an OH group that is attached to phenyl ring. The 5- or 6-membered aromatic or heteroaromatic ring or the phenyl ring can be part of a larger structure as defined below. In these embodiments L is suitable for binding to A via the O atom, giving a carbamate.
[0166] If L is
##STR00014##
then the cargo, which may be a therapeutic, diagnostic or theranostic agent (such as an aniline or amine) that contains an NH.sub.2 or NH moiety; or an OH group that is attached to a 5- or 6-membered aromatic or heteroaromatic ring that can be part of a larger structure as defined below; preferably, an OH group that is attached to a 5- or 6-membered aromatic ring that can be part of a larger structure as defined below; more preferably, an OH group that is attached to a phenyl ring that can be part of a larger structure as defined below.
[0167] In these embodiments the NH.sub.2 or NH moiety or the OH group is suitable for binding to L via the respective N or O atom.
[0168] If L is
##STR00015##
then the cargo, which is a therapeutic, diagnostic or theranostic agent, can contain an NH.sub.2 or NH moiety; or an OH group that is attached to a 5- or 6-membered aromatic or heteroaromatic ring; preferably an NH.sub.2 or NH moiety which is attached to an aromatic ring that can be part of a larger structure as defined below, or an OH group that is attached to a 5- or 6-membered aromatic ring that can be part of a larger structure as defined below; more preferably an OH group that is attached to a phenyl ring that can be part of a larger structure as defined below.
[0169] In these embodiments the NH.sub.2 or NH moiety or the OH group is suitable for binding to L via the respective N or O atom.
[0170] q is 0, 1, 2, 3 or 4, preferably q is 0 or 1.
[0171] Each group R is independently selected from -halogen O(R.sup.r), N(R.sup.s)(R.sup.t), NO.sub.2, CN, and a heterocyclic group selected from azetidinyl, pyrrolidinyl, piperidinyl or morpholino, wherein the heterocyclic group is attached to the aromatic ring via the N atom. Preferably, group R is a halogen (such as F or Cl) in ortho position relative to the bond to A; also preferably, group R is O(R.sup.r) in ortho position relative to the bond to B.
[0172] R.sup.r is selected from H and C.sub.1-4-alkyl, preferably C.sub.1-4-alkyl, more preferably R.sup.r is methyl.
[0173] R.sup.s is selected from H, C(O)C.sub.1-4-alkyl and C.sub.1-4-alkyl, preferably H or C.sub.1-4-alkyl, more preferably H or CH.sub.3.
[0174] R.sup.t is selected from H, C(O)C.sub.1-4-alkyl and C.sub.1-4-alkyl, preferably H or C.sub.1-4-alkyl, more preferably H or CH.sub.3.
[0175] Most preferred examples of self-immolative spacers that link groups A and B are
##STR00016## [0176] B is
##STR00017##
[0177] in which denotes the attachment point from L to B.
[0178] A.sup.1 is selected such that A.sup.1-OH is a therapeutic, diagnostic or theranostic agent which contains an OH group that is attached to a 5- or 6-membered aromatic or heteroaromatic ring, preferably an OH group that is attached to a 5- or 6-membered aromatic ring, more preferably an OH group that is attached to a phenyl ring. The bond attaching L to that O atom replaces its bond to hydrogen that is present in the agent A.sup.1-OH. The 5- or 6-membered aromatic or heteroaromatic ring can be part of a larger structure which forms the therapeutic, diagnostic or theranostic agent A.sup.1-OH. In particular, the 5- or 6-membered aromatic or heteroaromatic ring can be substituted, or can be fused to a further ring or rings such as aromatic or heteroaromatic rings, preferably phenyl, pyridinyl, naphthyl, quinolyl, isoquinolyl, indolyl, pyrrolyl, thiophenyl, pyridinyl, pyrazolyl, pyrimidinyl, furanyl, oxazolyl, thiazolyl, imidazolyl, purinyl, pyrrolo, benzofuranyl, benzothiophenyl, benzothiazolyl, benzoxazolyl or naphthyridinyl, quinoxalyl, benzopyranonyl, benzo[1,3]dioxolyl, benzothiazolyl, 2,3-dihydro-1H-benzo[e]indole, xanthenyl or indolyl, more preferably phenyl, pyridinyl, benzopyranonyl, naphthyl, benzo[1,3]dioxolyl, 2,3-dihydro-1H-benzo[e]indole, quinolyl or xanthenyl.
[0179] A.sup.1 can be any hydrocarbon moiety which contains a 5- or 6-membered aromatic or heteroaromatic ring, wherein the hydrocarbon moiety has from 3 to 50 carbon atoms, preferably from 5 to 50 carbon atoms, more preferably 6 to 50 carbon atoms. The hydrocarbon moiety can optionally contain 1 or more heteroatoms, preferably 1 to 20 heteroatoms, more preferably 1 to 15 heteroatoms, wherein the heteroatoms can be selected from O, N, S, Se, Si, Hal, B or P, preferably selected from O, N, Hal, S, Si or P, more preferably selected from O, N, Hal, S, Si, even more preferably selected from O, N or Hal.
[0180] A.sup.2 and A.sup.3 are independently selected such that A.sup.2-NH-A.sup.3 is a therapeutic, diagnostic or theranostic agent which contains an NH.sub.2 or NH moiety. A.sup.2 and A.sup.3 can be independently selected from 5- or 6-membered aromatic or heteroaromatic rings and aliphatic moieties, and A.sup.2 may optionally be H. In one embodiment, A.sup.2 and A.sup.3 may optionally be connected forming a carbocyclic or heterocyclic ring that can be saturated, unsaturated or aromatic and which has from 5 to 7 ring atoms. Each 5- or 6-membered aromatic, heteroaromatic, carbocyclic or heterocyclic ring can be part of a larger structure which forms the therapeutic, diagnostic or theranostic agent A.sup.2-NH-A.sup.3. In particular, the 5- or 6-membered aromatic or heteroaromatic ring or the carbocyclic or heterocyclic ring can be substituted or can be fused to a further ring or rings such as aromatic or heteroaromatic rings, preferably phenyl, pyridinyl, naphthyl, quinolyl, isoquinolyl, indolyl, pyrrolyl, thiophenyl, pyridinyl, pyrazolyl, pyrimidinyl, furanyl, oxazolyl, thiazolyl, imidazolyl, purinyl, pyrrolo, benzofuranyl, benzothiophenyl, benzothiazolyl, benzoxazolyl or naphthyridinyl, quinoxalyl, benzopyranonyl, benzothiazolyl, xanthenyl or indolyl. The aliphatic moiety can be substituted.
[0181] A.sup.2 can be hydrogen or any hydrocarbon moiety which has from 1 to 50 carbon atoms, preferably from 1 to 20 carbon atoms, more preferably 1 to 10 carbon atoms, even more preferably 1 to 4 carbon atoms. The hydrocarbon moiety can optionally contain 1 or more heteroatoms, preferably 1 to 10 heteroatoms, more preferably 1 to 5 heteroatoms, wherein the heteroatoms can be selected from O, N, S, Se, Si, Hal, B or P, preferably selected from O, N, Hal, S, Si or P, more preferably selected from O, N, Hal, S, Si, even more preferably selected from O, N or Hal. In one embodiment A.sup.2 is a C alkyl, such as methyl.
[0182] A.sup.3 can be any hydrocarbon moiety which contains from 3 to 50 carbon atoms, preferably from 5 to 50 carbon atoms, more preferably 6 to 50 carbon atoms. The hydrocarbon moiety can optionally contain 1 or more heteroatoms, preferably 1 to 20 heteroatoms, more preferably 1 to 15 heteroatoms, wherein the heteroatoms can be selected from O, N, S, Se, Si, Hal, B or P, preferably selected from O, N, Hal, S, Si or P, more preferably selected from O, N, Hal, S, Si, even more preferably selected from O, N or Hal.
[0183] If A.sup.2 and A.sup.3 are bound together to form a carbocyclic or heterocyclic ring, the carbocyclic or heterocyclic ring can contain from 3 to 50 carbon atoms, preferably from 5 to 50 carbon atoms, more preferably 6 to 50 carbon atoms. The hydrocarbon moiety can optionally contain 1 or more heteroatoms, preferably 1 to 20 heteroatoms, more preferably 1 to 15 heteroatoms, wherein the heteroatoms can be selected from O, N, S, Se, Si, Hal, B or P, preferably selected from O, N, Hal, S, Si or P, more preferably selected from O, N, Hal, S, Si, even more preferably selected from O, N or Hal.
[0184] The nature of the therapeutic agent, diagnostic agent or theranostic agent is not particularly limited, as long as it contains an OH, NH.sub.2 or NH moiety which after removal of the H is suitable for binding to the self-immolative spacer L, or, if L is a bond, to A, via the O or N atom. As can be seen from the schemes below, the O or N atom partakes in the reaction when the moiety A of a compound having the formula (I) is cleaved by an oxidoreductase or redox effector protein. The rest of the therapeutic agent, diagnostic agent or theranostic agent is not involved in this reaction, so that a wide variety of therapeutic agents, diagnostic agents and theranostic agents are suitable for use in the present invention.
[0185] Examples of suitable therapeutic agents, diagnostic agents and theranostic agents include, but are not limited to diagnostically acceptable dyes, therapeutically acceptable DNA-alkylating agents, therapeutically acceptable topoisomerase-inhibiting agents or therapeutically acceptable tubulin-inhibiting agents. In one embodiment, diagnostically acceptable dyes are preferred, which includes compounds or structurally and functionally related derivatives selected from, but not limited to the following classes of dyes: xanthenes including e.g. alkyl-fluoresceins, alkyl-carbofluoresceins, alkyl-silarhodamines, phenylquinazolinones, coumarins, firefly-luciferins, black hole quencher dyes, or any compound derived by substitution of the same. How acceptable compounds may be derived by substitution will be clarified in the subsequent examples. In another embodiment, therapeutically acceptable agents are preferred, which includes theranostic agents that include structurally and functionally related derivatives of the leuco-methylene blue class; DNA-alkylating agents that include compounds or structurally and functionally related derivatives selected from, but not limited to the following classes: nitrogen mustards or seco-cyclopropabenzaindoles; topoisomerase-inhibiting agents that include compounds or structurally and functionally related derivatives selected from, but not limited to the following classes of compounds: anthracyclins or camptothecins; antiproliferative agents that include compounds or structurally and functionally related derivatives selected from, but not limited to the following classes: monomethyl auristatins, the Amaryllidaceae alkaloids; or any compounds derived by substitution of the same.
[0186] Examples of therapeutic agents, diagnostic agents and theranostic agents include, but are not limited to, agents for treating, ameliorating, preventing or diagnosing a neoplastic disorder, an inflammatory disorder (e.g. a non-steroidal anti-inflammatory drug (NSAID), a disease-modifying anti-rheumatic drug (DMARD), or mesalamine), atherosclerosis; an autoimmune disorder; a chronic inflammatory autoimmune disorder; ischaemia; and reperfusion injury as well as kinase inhibitors.
[0187] Preferred are agents for which the conjugation of the active agent H-B into the compound A-L-B results in a substantial reduction of diagnostic signal or therapeutic potency; particularly preferred are therapeutically acceptable agents for which the conjugation of the active agent H-B into the compound A-L-B totally or near-totally prevents the compound from exhibiting the diagnostic signal or therapeutic potency that is associated with the free agent H-B. This preferred total or near-total prevention of the activity of agent H-B when conjugated as A-L-B is possible in several ways, non-limiting examples of which are given below in the section Utility, Diseases, and Examples.
[0188] How suitable diagnostic, therapeutic or theranostic agents may be derived by substitution of specific agents will be understood by reference to the following non-limiting examples: [0189] A.sup.1-OH can be Haugland's precipitating fluorophore
##STR00018##
[0190] A.sup.1-OH or A.sup.2-NH-A.sup.3 can be a diagnostic agent of the xanthene class:
##STR00019##
[0191] L.sup.1, L.sup.2, L.sup.3 or L.sup.4 are independently selected from H, -halogen, NO.sub.2, CN, OMe, OCF.sub.3.
[0192] L.sup.5 is attached at any free position on the indicated benzene ring and is selected from H, C.sub.1-4-alkyl, -halogen, C(O)C.sub.1-4-alkyl, C(O)OC.sub.1-4-alkyl, C(O)N(C.sub.1-4-alkyl).sub.2, S(O).sub.2OH, S(O).sub.2OC.sub.1-4-alkyl, OC.sub.1-4-alkyl.
[0193] G.sup.1 is selected from O, S, Se, C(C.sub.1-4-alkyl).sub.2-, Si(C.sub.1-4-alkyl).sub.2-.
[0194] G.sup.2 is selected from CH.sub.2, C(O), S(O).sub.2, P(O).sub.2.
[0195] G.sup.3 is selected from O, N(C.sub.1-4-alkyl)-.
[0196] G.sup.4 is selected from OH, OC.sub.1-4-alkyl, NH.sub.2, NHC.sub.1-4-alkyl, N(C.sub.1-4-alkyl).sub.2, NC(O)C.sub.1-4-alkyl, wherein any of the C.sub.1-4-alkyl in G.sup.4 can be optionally substituted by W.
[0197] R.sup.u is selected from H, C.sub.1-4-alkyl.
[0198] When A.sup.1 is selected such that A.sup.1-OH is a diagnostic agent of the phenylquinazolinone class:
##STR00020## [0199] G.sup.5 is selected from CH.sub.2, CH?CH, O, S, Se.
[0200] One or more substituent L.sup.6 and one or more substituent L.sup.7 are independently selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2.
[0201] When A.sup.1 is selected such that A.sup.1-OH is a diagnostic agent of the umbelliferone class:
##STR00021## [0202] L.sup.8 is selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2. [0203] L.sup.9 is selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2. [0204] L.sup.10 is selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2.
[0205] When A.sup.1, A.sup.2 or A.sup.3 are selected such that A.sup.1-OH or A.sup.2-NH-A.sup.3 is a therapeutic agent of the seco-cyclopropabenzaindole (CBI) class of DNA-alkylating anti-cancer drugs:
##STR00022## [0206] G.sup.6 is selected from Cl, Br, I, OMs, OTs, OTf, ONs. [0207] G.sup.7 is selected from H, C.sub.1-4-alkyl, CF.sub.3. [0208] G.sup.8 is selected from CH?CH, O, S. [0209] One or more substituent L.sup.11 is independently selected from H, F, C.sub.1-4-alkyl, CF.sub.3, OMe, OC.sub.1-4-alkyl. [0210] L.sup.12 and L.sup.13 are independently selected from H, F, C.sub.1-4-alkyl, CF.sub.3, OMe, OC.sub.1-4-alkyl, wherein any of the C.sub.1-4-alkyl in L.sup.12 and L.sup.13 can be optionally substituted by W. [0211] L.sup.14 is selected from OC.sub.1-4-alkyl optionally substituted by W.
[0212] When A.sup.2 and A.sup.3 are selected such that A.sup.2-NH-A.sup.3 is a diagnostic or theranostic agent of the leuco-methylene blue class:
##STR00023## [0213] G.sup.8 is selected from S, Se, Si(C.sub.1-4-alkyl).sub.2-, Ge(C.sub.1-4-alkyl).sub.2-. [0214] G.sup.9 is selected from NH.sub.2, NH(C.sub.1-4-alkyl), N(C.sub.1-4-alkyl).sub.2. [0215] L.sup.15, L.sup.16 and L.sup.17 are independently selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2.
[0216] When A.sup.1 is selected such that A.sup.1-OH is a therapeutic agent of the camptothecin class of topoisomerase-inhibiting anti-cancer drugs:
##STR00024## [0217] G.sup.10 is selected from H, C(O)C.sub.alkyl. [0218] One or more substituent L.sup.18 are independently selected from H, F, CF.sub.3, CH.sub.2CF.sub.3, C.sub.1-4-alkyl, CH.sub.2N(C.sub.1-4-alkyl).sub.2. [0219] L.sup.19 is selected from H, F, CF.sub.3, CH.sub.2CF.sub.3, C.sub.1-4-alkyl, CH.sub.2N(C.sub.1-4-alkyl).sub.2. [0220] L.sup.20 is selected from CF.sub.3, CH.sub.2CF.sub.3, C.sub.1-4-alkyl.
[0221] When A.sup.1 or A.sup.2 and A.sup.3 are selected such that A.sup.1-OH or A.sup.2-NH-A.sup.3 is a therapeutic agent of the nitrogen mustard class of DNA-alkylating agents:
##STR00025## [0222] G.sup.1 and G.sup.12 are independently selected from Cl, Br, I, OMs, OTs, OTf, ONs. [0223] L.sup.21 and L.sup.22 are independently selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2.
[0224] When A.sup.1 is selected such that A.sup.1-OH is a diagnostic agent of the firefly luciferin class:
##STR00026## [0225] One or more substituent L.sup.23 is selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2.
[0226] When A.sup.1 is selected such that A.sup.1-OH is a therapeutic agent of the Amaryllidaceae class of anti-proliferative alkaloids:
##STR00027## [0227] L.sup.24 is selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2. [0228] J.sup.1 and J.sup.6 are independently selected from CH or ?C. [0229] J.sup.2, J.sup.3, J.sup.4 and J.sup.5 are independently selected from CH.sub.2, CH(OH), CH(O(C(O)C.sub.1-4-alkyl))-, CH(O-(galactoside)-, CH(O-(glucoside)- or ?CH.
[0230] When A.sup.1 is selected such that A.sup.1-OH is a diagnostic agent of the Black hole quencher class:
##STR00028## [0231] One or more substituent L.sup.25 is independently selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2. [0232] L.sup.26 and L.sup.27 are independently selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, CF.sub.3, NO.sub.2. [0233] L.sup.28 is selected from H, C.sub.1-4-alkyl, -halogen, OC.sub.1-4-alkyl, N(C.sub.1-4-alkyl).sub.2, CF.sub.3, NO.sub.2. [0234] G.sup.13 is selected from phenyl, pyridine-2-yl, pyridine-3-yl, pyridine-4-yl, 2-methoxyphenyl, 3-methoxyphenyl, 4-methoxyphenyl, 3,5-dimethoxyphenyl, 2,4-dimethoxyphenyl, 2,3,4-trimethoxyphenyl, pyrazin-2-yl.
[0235] When A.sup.2 and A.sup.3 are selected such that A.sup.2-NH-A.sup.3 is a therapeutic agent of the anthracycline class:
##STR00029## [0236] G.sup.14 is selected from H, C.sub.1-4-alkyl, OH, NH.sub.2, OC.sub.1-4-alkyl, NH(C.sub.1-4-alkyl), N(C.sub.1-4-alkyl).sub.2, CF.sub.3. [0237] G.sup.15 is selected from H, C.sub.1-4-alkyl, OH, NH.sub.2. [0238] J.sup.7 is selected from H, C.sub.1-4-alkyl, OH, NH.sub.2, OC.sub.1-4-alkyl, CF.sub.3. [0239] J.sup.8 is selected from H, C.sub.1-4-alkyl, OH, O-(tetrahydro-2H-pyran-2-yl), NH.sub.2, OC.sub.1-4-alkyl, CF.sub.3, CH.sub.2, C(C.sub.1-4-alkyl).sub.2-, O, S. [0240] If J.sup.8 is selected from H, C.sub.1-4-alkyl, OH, O-(tetrahydro-2H-pyran-2-yl), NH.sub.2, OC.sub.1-4-alkyl, CF.sub.3, then J.sup.9 is selected from CH.sub.2, C(C.sub.1-4-alkyl).sub.2-. [0241] If J.sup.8 is selected from CH.sub.2, C(C.sub.1-4-alkyl).sub.2-, O, S, then J.sup.9 is selected from CH.sub.2, C(C.sub.1-4-alkyl).sub.2-. [0242] One or more substituent L.sup.21 is independently selected from OC.sub.1-4-alkyl, OH, -halogen, NH.sub.2, OC.sub.1-4-alkyl, CF.sub.3.
[0243] When A.sup.2 and A.sup.3 are selected such that A.sup.2-NH-A.sup.3 is a therapeutic agent of the auristatin class:
##STR00030## [0244] J.sup.10 is selected from H, C.sub.1-4-alkyl, OH, NH.sub.2, OC.sub.1-4-alkyl. [0245] J.sup.11 is selected from H, C.sub.1-4-alkyl, OH, NH.sub.2, OC.sub.1-4-alkyl, C(O)H, C(O)OC.sub.1-4-alkyl, thiazo-2-yl, thiazo-4-yl, oxazo-2-yl, oxazo-4-yl. [0246] J.sup.12 is selected from H, C.sub.1-4-alkyl. [0247] J.sup.13 is selected from H, C.sub.1-4-alkyl.
[0248] Illustrative embodiments of the compound having the formula (I) include
##STR00031## ##STR00032## ##STR00033## ##STR00034##
[0249] It is understood that all combinations of the above definitions and preferred definitions are envisaged by the present inventors.
Synthesis of the Compounds Having the Formula (I)
A) General Manufacturing of the Dichalcogenide-Containing Moiety A
[0250] In some embodiments, general manufacturing of the compound of the invention can begin from a diol precursor similar to diol A1, which itself can be prepared from commercially available starting materials using literature known procedures.
##STR00035##
[0251] PG.sup.1 represents any protecting group for the heterocyclic nitrogen atom, which intermediately masks the nitrogen's nucleophilic reactivity and can be cleaved without affecting other functional groups in the molecule. Preferably PG.sup.1 can be selected from any of the following N-protecting groups: acetamide (Ac), trifluoroacetamide, tert-butyl carbamate (Boc), benzyl carbamate (Cbz), trityl (Trt), dimethoxy-trityl (DMT), toluenesulfonyl (Ts), o-nitro benzenesulfonyl (Ns), 9-fluorenmethyl carbamate (Fmoc), p-methoxy benzyl carbamate (Moc), benzoyl (Bz), 3,4-dimethoxybenzyl (DMPM), p-methoxybenzyl (PMB), p-methoxyphenyl (PMP), and trichloroethyl carbamate (Troc). More preferably PG.sup.1 can be selected from one of the following N-protecting groups with preferred compatibility: acetamide (Ac), trifluoroacetamide, tert-butyl carbamate (Boc), trityl (Trt), dimethoxy-trityl (DMT), 9-fluorenmethyl carbamate (Fmoc), and trichloroethyl carbamate (Troc). Most preferably PG.sup.1 can be selected from of following N-protecting groups with preferred compatibility and accessibility: acetamide (Ac), and tert-butyl carbamate (Boc).
[0252] Oxo-sulfur or oxo-selenium substitution can be generally achieved by any substitution reaction using any sulfur or selenium nucleophile and any activation method for alcohols. Preferably, oxo-sulfur substitution can be achieved by using (1) one of the following activation reagents transforming the alcohols into reactive LG.sup.1 and LG.sup.2: p-toluenesulfonyl chloride (TsCl), methanesulfonyl chloride (MsCl), trifluoromethanesulfonic anhydride (Tf.sub.2O), dialkyl azodicarboxylate activated with a trialkyl- or triarylphosphine (Mitsunobu conditions: e.g. PPh.sub.3/DIAD), tetrahalogenmethane activated with a trialkyl- or triarylphosphine (e.g. PPh.sub.3/CBr.sub.4); and (2) one of the following nucleophiles: thioacetic acid (HSAc) or its sodium or potassium salt (NaSAc or KSAc), thiobenzoic acid (HSBz) or its sodium or potassium salt (NaSBz or KSBz), p-nitro-thiobenzoic acid or its sodium or potassium salt, tert-butyl mercaptan (HS-tBu), benzyl mercaptan (HS-Bn), p-methoxybenzyl mercaptan (HS-PMB), methanethiol (HS-Me), sodium or potassium thiocyanate or any alkyl thiol (HS-alkyl). Preferably oxo-sulfur substitution can be achieved by using MsCl or TsCl then KSAc in two separate steps or using thio-Mitsunobu conditions (PPh.sub.3/DIAD followed by thioacetic acid, thiobenzoic acid, or p-nitro-thiobenzoic acid in one step). More preferably oxo-sulfur substitution can be achieved by using thio-Mitsunobu conditions of PPh.sub.3/DIAD followed by thioacetic acid in one step.
[0253] Preferably, oxo-selenium substitution can be achieved by using (1) one of the following activation reagents transforming the alcohols into reactive LG.sup.1 and LG.sup.2: TsCl, MsCl, Tf.sub.2O, dialkyl azodicarboxylate activated with a trialkyl- or triarylphosphine (Mitsunobu conditions: e.g. PPh.sub.3/DIAD), tetrahalogenmethane activated with a trialkyl- or triarylphosphine (e.g. PPh.sub.3/CBr.sub.4); and (2) one of the following nucleophiles: selenoacetic acid (HSeAc) or its sodium or potassium salt (NaSeAc or KSeAc), selenobenzoic acid (HSeBz) or its sodium or potassium salt (NaSeBz or KSeBz), p-nitro-selenobenzoic acid or its sodium or potassium salt, tert-butyl selenol (HSe-tBu), benzyl selenol (HSe-Bn), p-methoxybenzyl selenol (HSe-PMB) or its sodium or potassium salt (NaSePMB or KSePMB), methaneselenol (HS-Me), sodium or potassium thiocyanate or any alkyl thiol (HS-alkyl). Preferably, oxo-selenium substitution can be achieved by using mesylation or tosylation followed by sodium or potassium selenocyanate, sodium or potassium p-methoxybenzyl selenolate (NaSePMB or KSePMB) or selenium thioacetate in two separate steps or using seleno-Mitsunobu conditions (PPh.sub.3/DIAD followed by selenoacetic acid, selenobenzoic acid, or p-nitro-selenobenzoic acid in one step). More preferably oxo-selenium substitution can be achieved by using mesylation or tosylation followed by sodium or potassium selenocyanate.
[0254] The groups PG.sup.2 and PG.sup.3 represent the protecting groups for the selenium and/or sulfur atoms which are installed in A3.
[0255] The groups PG.sup.2 and PG.sup.3 and Z.sup.1 and Z.sup.2 which are installed in A3 by these reactions depend on the reagent or sequence of reagents and the number of equivalents of those reagents used for the oxo-sulfur or oxo-selenium substitutions, as will be known to those skilled in the art. For example, when oxo-selenium substitution is performed on both alcohol groups simultaneously with excess NaSePMB as the selenium nucleophile, the resulting A3 will have PG.sup.2=PG.sup.3=PMB and Z.sup.1=Z.sup.2=Se. Alternatively, when both alcohol groups are activated in one step but nucleophilically substituted in two separate steps with two different nucleophiles, regioisomeric products that may be separable may be obtained. For example, activating both alcohols with MsCl, and treating the product with 1 equivalent of KSAc, can depending on the conditions and the protecting groups used, allow isolation of a pair of regioisomers of the mono-S-acetylated product, and these may then be converted by reaction with e.g. KSeCN to a pair of products where one has PG.sup.2=Ac, Z.sup.1=S, PG.sup.3?CN, Z.sup.2=Se and the other has PG.sup.2?CN, Z.sup.1=Se, PG.sup.3=Ac, Z.sup.2=S.
[0256] Depending on the selected conditions for oxo-sulfur substitution, the following sulfur protecting groups can be obtained: acetate (Ac), benzoate (Bz), p-nitro benzoate, tert-butyl, methyl, alkyl, cyanide (CN) or p-methoxybenzyl; preferably Ac, Bz, p-nitro benzoate; more preferably Ac.
[0257] Depending on the selected conditions for oxo-selenium substitution, the following selenium protecting groups can be obtained: cyanide (CN), Ac, Bz, p-nitro benzoate, tert-butyl, methyl, alkyl or p-methoxy benzyl; preferably CN, Ac, Bz, p-nitro benzoate or p-methoxy benzyl; more preferably CN.
[0258] Deprotection of PG.sup.2 and/or PG.sup.3 in A3 can generally be achieved applying any appropriate deprotection conditions, which depending on PG.sup.1 and/or PG.sup.2/PG.sup.3 may be selected from the following list of general deprotection conditions: (1) acidic deprotection: solutions of HCl in organic solvents (HCl/MeOH, HCl/EtOH, HCl/dioxane, HCl/Et.sub.2O), or carboxylic acids (trifluoroacetic acid (TFA), trichloroacetic acid); (2) basic deprotection: piperidine, NaOH, KOH, Na.sub.2CO.sub.3, K.sub.2CO.sub.3, or Cs.sub.2CO.sub.3; (3) oxidative deprotection: 1,2-dichloro-4,5-dinitro-quinone (DDQ), cerium(IV) ammonium nitrate (CAN), tetrapropylammonium perruthenate, potassium perruthenate; (4) reductive deprotection: hydrogenolysis (H.sub.2 in presence of Pd/C), diisobutylaluminium hyride (DIBAL-H), Na/NH.sub.3, Li/naphthalene, Zn/HCl, LiAlH.sub.4, NaBH.sub.4, NaCNBH.sub.3. Preferred conditions are: (1) acidic deprotection: solutions of HCl in organic solvents (HCl/MeOH, HCl/EtOH, HCl/dioxane, HCl/Et.sub.2O) or carboxylic acids (trifluoroacetic acid (TFA), trichloroacetic acid); (2) basic deprotection: piperidine, KOH, or K.sub.2CO.sub.3. More preferably, acidic deprotection conditions are HCl/dioxane and basic deprotection conditions are KOH.
[0259] Deprotection conditions depending on PG.sup.1 and/or PG.sup.2 and/or PG.sup.3 can be selected, so that (1) PG.sup.1, PG.sup.2 and PG.sup.3 are cleaved consecutively giving the free dichalcogenol with a free amine, (2) so that only PG.sup.2 and PG.sup.3 are cleaved giving the free dichalcogenol leaving the N-protecting group PG.sup.1 intact. Alternatively, (3) only one of PG.sup.2 or PG.sup.3 are cleaved, giving an N-protected dichalcogenol with one chalcogenol (either Z or Z.sup.2) still protected. Alternatively, (4) PG.sup.1 is cleaved and only one of PG.sup.2 or PG.sup.3 are cleaved, giving a dichalcogenol with a free amine and one chalcogenol (either Z.sup.1 or Z.sup.2) still protected.
[0260] Ring closure (formation of the dichalcogenide) can be accomplished in two ways, and based on the deprotection method employed to cleave PG.sup.1 and/or PG.sup.2 and/or PG.sup.3.
[0261] If deprotection conditions (1) apply, ring closure can be executed by any 2-electron oxidation conditions suitable for dichalcogenol-to-dichalcogenide oxidation to give dichalcogenide A5 directly. Preferably these 2-electron oxidation conditions are selected from the following reaction conditions: I.sub.2/MeOH, I.sub.2/EtOH, I.sub.2/DCM, O.sub.2/MeOH, O.sub.2/EtOH, O.sub.2/DCM, DMSO. More preferably these are selected from the following conditions: I.sub.2/MeOH or I.sub.2/DCM.
[0262] If deprotection conditions (2) apply, ring closure can be executed by any 2-electron oxidation conditions as defined above to give dichalcogenide A4 directly.
[0263] If deprotection conditions (3) apply, ring closure can occur under the applied deprotection conditions to give dichalcogenide A4 directly from A3.
[0264] If deprotection conditions (4) apply, ring closure can occur under the applied deprotection conditions to give dichalcogenide A5 directly from A3.
[0265] Deprotection of A4 can generally be achieved applying any appropriate deprotection conditions to give A5, which depending on PG.sup.1 may be selected from the List of General Deprotection Conditions outlined above.
[0266] A5 is obtained as a free amine or any acceptable ammonium salt of the same which may be selected from, but not limited to the following: acetate, benzoate, bromide, chloride, formate, iodide, nitrate, sulfate, or trifluoroacetate salt.
[0267] In some embodiments, general manufacturing of the compound of the invention can begin from diamine A18, which may be transformed to A19 by any appropriate conditions, such as by reductive amination. Depending on the nature of X and Y, 6-membered piperazine or 7-membered 1,4-diazepane ring structures can be prepared by applying the respective optionally substituted dialdehyde, diketone, or ketoaldehyde precursors, which may be selected, but are not limited to the following: glyoxal, malondialdehyde, butanedione, and pentane-2,4-dione, or technically acceptable formulations which release them in situ. For reduction of the intermediate cyclic diimine structure any reducing agent may be used.
[0268] Preferably, a reducing agent may be selected from but is not limited to: LiAlH.sub.4, LiBH.sub.4, NaBH.sub.4, NaCNBH.sub.3, NaBH(OAc).sub.3, KBH.sub.4, LiBHEt.sub.3 or Ti(BH.sub.4).sub.4(OEt.sub.2). More preferably the cyclic diimine structure may be reduced using NaBH.sub.4 or NaCNBH.sub.3, most preferably using NaCNBH.sub.3.
##STR00036##
[0269] Monofunctionalisation of A19 to achieve A20 may be achieved by any N-alkylation, N-acylation or N-sulfonylation reaction with appropriate reagents, optionally with additional use of protecting groups, as necessary, and as known to those skilled in the art. Depending on the nature of R.sup.a, A19 may be treated with commercially available reagents, including but not limited to the following: For acylation: acetyl chloride, pivaloyl chloride, iPrC(O)Cl, nBuC(O)Cl, methyl chloroformate, dimethyl carbamoylchloride; for sulfonylation: methanesulfonyl chloride; for alkylation: 3-(dimethylamino)propyl chloride, 3-(piperidin-1-yl)propyl chloride, 4-(azetidin-1-yl) butyl chloride, 1-(4-chlorobutyl)azetidine, 1-(3-chloropropyl)pyrrolidine, 2-(2-chloro-ethoxy)ethan-1-ol, methyl iodide, ethyl iodide. Depending on the nature of R.sup.b, A20 may be coupled with a proteinogenic amino acid to prepare a monopeptide thereof. Peptide coupling may be achieved by any appropriate coupling conditions for coupling of secondary amines with carboxylic acids. Preferably, peptide coupling may be achieved by using one of the following coupling reagents: DCC, DIC, EDCI, HATU, HBTU, PyBOP or 1-hydroxybenzotriazole. Preferably, peptide coupling may be achieved by using DCC or EDCI, most preferably by using EDCI.
B) General Manufacturing: Compounds with No Self-Immolative Linker
[0270] ##STR00037##
[0271] The phenolic structure A6 represents a phenolic substructure of a motif which may be a therapeutic, diagnostic or theranostic cargo. Transformation of the phenolic alcohol function of A6 into an activated carbonate derivative may be achieved by any appropriate method for the transformation of alcohols into activated carbonate derivatives.
[0272] Methods for the transformation of alcohols into activated carbonate derivatives include using one of the following reagents: phosgene, diphosgene (DP), triphosgene (TP), 1,1-carbodiimidazole (CDI), N,N-disuccinimidyl carbonate (DSC), di-2-pyridyl carbonate (DPC), dimethyl carbonate (DMC), bis(p-nitrophenyl) carbonate (DNPC), bis (p-chlorophenyl) carbonate (DCPC), phenyl chloroformate, p-nitrophenyl chloroformate, p-chlorophenyl chloroformate, or ethylene carbonate with chlorine. Preferably, the reagent is DP, TP, CDI, or p-chlorophenyl chloroformate. More preferably, the reagent is TP.
[0273] LG.sup.3 depends on the selected method for the transformation of the phenolic alcohol function of A6 into an activated carbonate derivative and preferably may be one of the following: chloro, N-imidazolyl, N-methyl-N-imidazoliumyl, succinimidyl, 2-pyridyl, methoxy, p-nitrophenyloxy, p-chlorophenyloxy, or phenyloxy. More preferably, LG.sup.3 may be one of the following: chloro, N-imidazolyl, or p-chlorophenyloxy. Most preferably, LG.sup.3 is chloro.
[0274] The activated carbonate derivative A7 is coupled with the free amine A5 or any acceptable ammonium salt of the same to yield A8 by applying any appropriate reagent Suitable for Carbamate Coupling, which may be selected from the following: a trialkylamine (such as NMe.sub.3, NEt.sub.3, diisopropylethylamine (DIPEA), isopropyldiethylamine, triisopropylamine), pyridine, or 4-(N,N-dimethylamino)pyridine (DMAP); preferably, NEt.sub.3 or DIPEA.
C) General Manufacturing: Compounds Including a Benzylic Self-Immolative Linker
[0275] ##STR00038##
[0276] A9 can be used to introduce an appropriate self-immolative spacer for the release of cargos. The protected benzylic alcohol can either be in para (1,4) or ortho (1,2) position relative to the phenolic alcohol. A9 can be prepared from e.g. commercially available 2-hydroxybenzyl alcohol or 4-hydroxybenzyl alcohol using literature known methods.
[0277] PG.sup.4 represents any protecting group for the benzylic alcohol, which intermediately masks the oxygen's nucleophilic reactivity as needed. Preferably PG.sup.4 may be one of the following O-protecting groups: methoxymethyl ether (MOM), tetrahydropyranyl ether (THP), tert-butyl ether (tBu), allyl ether, benzyl ether (Bn), tert-butyldimethylsilyl ether (TBS), tert-butyldiphenylsilyl ether (TBDPS), benzoate (Bz), acetate (Ac), pivalate (Piv). More preferably, PG.sup.4 can be selected from any of the following O-protecting groups: MOM, THP, tBu, TBS.
[0278] Transformation of the phenolic alcohol function of A9 into an activated carbonate derivative A10 may be achieved by any appropriate method for the transformation of alcohols into activated carbonate derivatives, as outlined above. LG.sup.3 depends on the selected method, as outlined above.
[0279] The activated carbonate A10 is coupled with the free amine A5 or any acceptable ammonium salt of the same by applying any reagent suitable for carbamate coupling, as outlined above, yielding A11.
[0280] Deprotection of A11 can generally be achieved by applying any appropriate deprotection conditions to give A12, which depending on PG.sup.4 may be selected from the General Deprotection Conditions as outlined above or from organic hydrogen fluoride solutions or any other hydrogen fluoride or fluoride source (HF/pyridine, alkali fluorides, alkaline earth fluorides, tetraalkylammonium fluorides). Preferred conditions are: organic hydrogen fluoride solutions or any other hydrogen fluoride or fluoride source (HF/pyridine, alkali fluoride, alkaline earth fluoride, tetraalkylammonium fluoride such as TBAF). More preferred are HF/pyridine or TBAF.
[0281] Activation of the benzylic alcohol A12 for nucleophilic substitution may be achieved by any activation method for alcohols. Preferably, this may be achieved using an activation reagent selected from the following list of Reagents for the Activation of Benzylic Alcohols: para-toluenesulfonyl chloride (TsCl), methanesulfonyl chloride (MsCl), trifluoromethanesulfonic anhydride (Tf.sub.2O), tetrachloromethane or tetrabromomethane or iodine activated with a trialkylphosphine or a triarylphosphine (e.g. PPh.sub.3/CBr.sub.4), thionyl chloride (SOCl.sub.2), phosphoryl chloride (POCl.sub.3), hydrochloric acid (HCl), or hydrobromic acid (HBr). Preferred are p-toluenesulfonyl chloride (TsCl), methanesulfonyl chloride (MsCl), thionyl chloride (SOCl.sub.2), phosphoryl chloride (POCl.sub.3), hydrochloric acid (HCl) or hydrobromic acid (HBr). Most preferred are methanesulfonyl chloride (MsCl) or hydrobromic acid (HBr).
[0282] Depending on the selected conditions for the activation of the benzylic alcohol, LG.sup.4 in A13 is any acceptable leaving group suitable for nucleophilic substitution, such as Cl, Br, I, para-toluenesulfonate (OTs), methylsulfonate (OMs), or trifluoromethylsulfonate (OTf). Preferably, LG.sup.4 is Br, Cl, or OMs. More preferably, LG.sup.4 is Br.
##STR00039##
[0283] A.sup.4 is selected, so that A6 is a therapeutic, diagnostic or theranostic cargo of which the OH group in A.sup.1-OH is a phenolic or aromatic hydroxyl group. A.sup.2 and A.sup.3 are selected, so that A14 is a therapeutic, diagnostic or theranostic cargo of which the NH group in A.sup.2-NH-A.sup.3 is an amine or aniline group.
[0284] The reaction of A13 with alcohol A6 yielding benzylic ethers A15a,b, or the reaction of A13 with amine A14 yielding benzylic amines A15c,d, can be achieved by any appropriate method for nucleophilic substitution, which includes the use of appropriate reagents for nucleophilic substitution, which may be selected from the following: (1) alkali or alkaline earth hydrides (e.g. NaH, CaH.sub.2), amides (e.g. NaNH.sub.2, LDA, LHMDS, KHMDS), alkoxides (e.g. KOtBu, NaOMe), hydroxides (e.g. NaOH, KOH), or carbonates (e.g. K.sub.2CO.sub.3, Cs.sub.2CO.sub.3); (2) trialkylamines (e.g.
[0285] NMe.sub.3, NEt.sub.3, DIPEA, isopropyldiethylamine, triisopropylamine) or 4-dimethylaminopyridine (DMAP). Preferred are hydrides (NaH, CaH.sub.2), hydroxides (NaOH, KOH), or carbonates (K.sub.2CO.sub.3, Cs.sub.2CO.sub.3). More preferred are NaH, KOH or K.sub.2CO.sub.3.
[0286] Alternatively, preparation of benzylic ethers A15a,b can be achieved from reaction of A6 with A12 using any appropriate conditions effecting in situ activation of the benzylic alcohol, including using dialkyl azodicarboxylate activated with a trialkylphosphine or triarylphosphine (Mitsunobu conditions: e.g. PPh.sub.3/DIAD).
D) General Manufacturing: Compounds Including a Benzyloxycarbonyl Self-Immolative Linker
[0287] ##STR00040##
[0288] A12 can also be used to introduce a benzyloxycarbonyl self-immolative spacer. Transformation of the benzylic alcohol function of A12 into the activated carbonate A16 may be achieved by any of the methods for the transformation of alcohols into activated carbonate derivatives outlined above. LG.sup.3 depends on the selected method, as outlined above.
##STR00041##
[0289] The activated carbonate A16 is coupled with aromatic alcohol A6 to yield A17a,b, or is coupled with amine A14 or any acceptable ammonium salt of the same to yield A17c,d, by applying any Reagent Suitable for Carbamate Coupling as outlined above.
[0290] Pharmaceutical or Diagnostic Compositions
[0291] The compounds of formula (I) can be administered to a patient in the form of a pharmaceutical or diagnostic composition which can optionally comprise one or more pharmaceutical carrier(s) or excipients.
[0292] The compounds of formula (I) can be administered by various well known routes, including oral, rectal and parenteral administration, e.g. intravenous, intramuscular, intradermal, subcutaneous, topical, and similar administration routes. Parenteral, oral and topical administration are preferred, particularly preferred is intravenous administration.
[0293] Particular preferred pharmaceutical or diagnostic forms for the administration of a compound of formula (I) are forms suitable for injectable use and include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. In all cases the final solution or dispersion form must be sterile and fluid. Typically, such a solution or dispersion will include a solvent or dispersion medium, containing, for example, water buffered aqueous solutions, e.g. biocompatible buffers, ethanol, polyol, such as glycerol, propylene glycol, polyethylene glycol, suitable mixtures thereof, surfactants or vegetable oils. A compound of the invention can also be formulated into liposomes, in particular for parenteral administration. Liposomes provide the advantage of increased half life in the circulation, if compared to the free drug and a prolonged more even release of the enclosed drug.
[0294] Sterilization of infusion or injection solutions can be accomplished by any number of art recognized techniques including but not limited to addition of preservatives like anti bacterial or anti-fungal agents, e.g. parabene, chlorobutanol, phenol, sorbic acid or thimersal. Further, isotonic agents, such as sugars or salts, in particular sodium chloride may be incorporated in infusion or injection solutions.
[0295] Production of sterile injectable solutions containing one or several of the compounds of the invention is accomplished by incorporating the respective compound in the required amount in the appropriate solvent with various ingredients enumerated above as required followed by sterilization. To obtain a sterile powder the above solutions are vacuum dried or freeze dried as necessary. Preferred diluents of the present invention are water, physiologically acceptable buffers, physiologically acceptable buffer salt solutions or salt solutions. Preferred carriers are cocoa butter and vitebesole. Excipients which can be used with the various pharmaceutical or diagnostic forms of a compound of the invention can be chosen from the following non limiting list: [0296] a) binders such as lactose, mannitol, crystalline sorbitol, dibasic phosphates, calcium phosphates, sugars, microcrystalline cellulose, carboxymethyl cellulose, hydroxyethyl cellulose, polyvinyl pyrrolidone and the like; [0297] b) lubricants such as magnesium stearate, talc, calcium stearate, zinc stearate, stearic acid, hydrogenated vegetable oil, leucine, glycerids and sodium stearyl fumarates, [0298] c) disintegrants such as starches, croscaramellose, sodium methyl cellulose, agar, bentonite, alginic acid, carboxymethyl cellulose, polyvinyl pyrrolidone and the like.
[0299] In one embodiment the formulation is for oral administration and the formulation comprises one or more or all of the following ingredients: pregelatinized starch, talc, povidone K 30, croscarmellose sodium, sodium stearyl fumarate, gelatin, titanium dioxide, sorbitol, monosodium citrate, xanthan gum, titanium dioxide, flavoring, sodium benzoate and saccharin sodium.
[0300] Other suitable excipients can be found in the Handbook of Pharmaceutical Excipients, published by the American Pharmaceutical Association, which is herein incorporated by reference.
[0301] The pharmaceutical or diagnostic compositions comprising a compound of the invention can be produced in a manner known per se to the skilled person as described, for example, in Remington's Pharmaceutical Sciences, 15.sup.th Ed., Mack Publishing Co., New Jersey (1991).
[0302] It is to be understood that depending on the severity of the disorder and the particular type which is treatable with one of the compounds of the invention, as well as on the respective patient to be treated, e.g. the general health status of the patient, etc., different doses of the respective compound are required to elicit a therapeutic or prophylactic effect.
[0303] The cargo is, as defined above, any therapeutic, diagnostic or theranostic agents. Depending on the therapeutic, diagnostic or theranostic agent, the mode of administration, amongst other factors, the appropriate dose spans a wide range. The determination of the appropriate dose lies within the discretion of the attending physician.
[0304] If desired, the pharmaceutical or diagnostic composition can comprise a second pharmaceutically active agent. The second pharmaceutically active agent is not limited and can be selected from a vascular disrupting agent, a cytotoxic chemotherapeutic agent and an immunomodulator. Examples thereof include combretastatin A-4 (CA4), 3-aminocombretastatin A-4, BNC105, ABT-751, ZD6126, combretastatin A-1, or prodrugs of the same (which includes but is not limited to combretastatin A-4 phosphate (CA4P), 3-aminocombretastatin A-4 3-serinamide (ombrabulin), combretastatin A-1 bisphosphate (CA1P), and BNC105 phosphate (BNC105P)), and pharmaceutically acceptable salts, solvates or esters of the same.
[0305] Mechanism of Reductive Release
[0306] The mechanism by which reductive cleavage of the dichalcogenide leads to release of the cargo agent, is shown in the following schemes depicting the release of A.sup.1-OH (phenol-type) or A.sup.2-NH-A.sup.3 (amine-type) cargos from compounds of the invention. Without wishing to be bound by theory, it is assumed that the reduction in cells of the dichalcogenide bond of the compounds of the invention could be performed by a reducing enzyme or protein with a dithiol or selenolthiol active site, for example, an oxidoreductase such as thioredoxin reductase 1 (TrxR1), or TrxR2, MsrB1, or GR; or an effector protein, such as thioredoxin 1 (Trx1), Trx2, Grx1, Grx2, PDIA1, DsbA, DsbB, or DsbC. [0307] 1. When L is a bond:
##STR00042##
[0308] When L is a bond, the phenolic cargo A.sup.1-OH is directly linked to A as a carbamate, giving compounds of type A8. Upon reduction of the dichalcogenide bond in A8, the reduced thiol/selenolate (for Z.sub.2=S and Z.sub.1=Se) or selenol/thiolate (for Z.sub.2=Se and Z.sub.1=S) or selenol/selenolate (for Z.sub.2=Z.sub.1=Se) species A21 is formed. A21 can intramolecularly cyclise via an addition/elimination mechanism, which irreversibly releases the free phenolic cargo (i.e. therapeutic, diagnostic or theranostic agent) A.sup.1-OH (A6) as well as the byproduct carbamoselenoate (for Z.sub.1=Se)/carbamothioate (for Z.sub.1=S) species A22. [0309] 2. When L is a benzylic (1,4)- or (1-6)-self-immolative linker which is linked to A as a phenolic carbamate, and is connected to B as a benzylic ether or benzylamine
##STR00043##
##STR00044##
[0310] General Mechanism: When L is a benzylic (1,4)- or (1,6)-self-immolative linker which is linked to A as a carbamate, and is connected to B as a benzylic ether or benzylamine, then B can be selected from phenol-type A.sup.1-OH or amine-type A.sup.2-NH-A.sup.3 cargos, giving compounds A15(a-d) as defined below. Upon reduction of the dichalcogenide bond in A15(a-d), the corresponding reduced thiol/selenolate (for Z.sub.2=S and Z.sup.1=Se) or selenol/thiolate (for Z.sub.2=Se and Z.sup.1=S) or selenol/selenolate (for Z.sub.2=Z.sup.1=Se) species A23(a-d) is formed. A23(a-d) can intramolecularly cyclise via an addition/elimination mechanism, which irreversibly releases the corresponding intermediate phenolic species A24(a-d) as well as carbamoselenoate (for Z.sup.1=Se)/carbamothioate (for Z.sup.1=S) byproduct A22. The nature of compounds A15(a-d) and how the corresponding intermediate A24(a-d) releases the cargo B are shown below:
[0311] When L is the (1,6)-self-immolative linker
##STR00045##
and B is the phenolic cargo
##STR00046##
##STR00047##
[0312] In compounds of type A15a, L is a benzylic (1,6)-self-immolative linker and B is a phenolic A1-OH cargo which is connected to the linker as a benzylic ether. Reduction of A15a gives A23a which after cyclisation gives intermediate A24a. A24a can perform an intramolecular 1,6-elimination releasing the quinone-methide species A25 and the free phenolic cargo A6. When L is the (1,4)-self-immolative linker
##STR00048##
and B is the phenolic cargo
##STR00049##
##STR00050##
[0313] In compounds of type A15b, L is a benzylic (1,4)-self-immolative linker and B is a phenolic A.sup.1-OH cargo, which is connected to the linker as a benzylic ether. Reduction of A15b gives A23b which after cyclisation gives intermediate A24b. A24b can perform an intramolecular 1,4-elimination releasing the quinone-methide species A26 and the free phenolic cargo A6.
[0314] When L is the (1,6)-self-immolative linker
##STR00051##
and B is the amine-type cargo
##STR00052##
##STR00053##
[0315] In compounds of type A15c, L is a benzylic (1,6)-self-immolative linker and B is an amine-type A.sup.2-NH-A.sup.3 cargo, which is connected to the linker as a benzylamine. Reduction of A15c gives A23c which after cyclisation gives intermediate A24c. A24c can follow an intramolecular 1,6-elimination releasing the quinone-methide species A25 and the free amine-type cargo (i.e., therapeutic, diagnostic or theranostic agent) A14.
[0316] When L is the (1,4)-self-immolative linker
##STR00054##
and B is the amine-type cargo
##STR00055##
##STR00056##
[0317] In compounds of type A15d, L is a benzylic (1,4)-self-immolative linker and B is an amine-type A.sup.2-NH-A.sup.3 cargo, which is connected to the linker as a benzylamine. Reduction of A15d gives A23d which after cyclisation gives intermediate A24d. A24d can perform an intramolecular 1,6-elimination releasing the quinone-methide species A26 and the free amine-type cargo A14. [0318] 3. When L is a benzylic (1,4)- or (1-6)-self-immolative linker which is linked to A as a phenolic carbamate, and is connected to B as a benzylic carbonate or carbamate
##STR00057##
##STR00058##
[0319] General Mechanism: When L is a benzylic (1,4)- or (1,6)-self-immolative linker which is linked to A as a carbamate, and is connected to B as a benzylic carbonate or carbamate, then B can be selected from phenol-type A.sup.1-OH or amine-type A.sup.2-NH-A.sup.3 cargos, giving compounds A16(a-d) as defined below. Upon reduction of the dichalcogenide bond in A16(a-d), the corresponding reduced thiol/selenolate (for Z.sub.2=S and Z.sub.1=Se) or selenol/thiolate (for Z.sub.2=Se and Z.sub.1=S) or selenol/selenolate (for Z.sub.2=Z.sub.1=Se) species A27(a-d) is formed. Depending on the redox microenvironment and local pH A27(a-d) can intramolecularly cyclise via an addition/elimination mechanism, which irreversibly releases the corresponding intermediate phenolic species A28(a-d) as well as carbamoselenoate (for Z.sub.1=Se)/carbamothioate (for Z.sub.1=S) byproduct A22. The nature of compounds A16(a-d) and how the corresponding intermediate A28(a-d) releases the cargo B are shown below:
[0320] When L is the (1,6)-self-immolative linker
##STR00059##
and B is the phenolic cargo
##STR00060##
##STR00061##
[0321] In compounds of type A16b, L is a benzylic (1,6)-self-immolative linker and B is a phenolic A.sup.1-OH cargo which is connected to the linker as a benzylic carbonate. Reduction of A16a gives A27a which after cyclisation gives intermediate A28a. A28a can perform an intramolecular 1,6-elimination releasing the quinone-methide species A25 as well as the carbonic acid species A29 which further eliminates carbon dioxide to give the free phenolic cargo A6. When L is the (1,4)-self-immolative linker
##STR00062##
and B is the phenolic cargo
##STR00063##
##STR00064##
[0322] In compounds of type A16b, L is a benzylic (1,4)-self-immolative linker and B is a phenolic A.sup.1-OH cargo which is connected to the linker as a benzylic carbonate. Reduction of A16b gives A27b which after cyclisation gives intermediate A28b. A28b can perform an intramolecular 1,4-elimination releasing the quinone-methide species A26 as well as the carbonic acid species A29 which further eliminates carbon dioxide to give the free phenolic cargo A6. When L is the (1,6)-self-immolative linker
##STR00065##
and B is the amine-type cargo
##STR00066##
##STR00067##
[0323] In compounds of type A16c, L is a benzylic (1,6)-self-immolative linker and B is an amine-type cargo A.sup.2-NH-A.sup.3 which is connected to the linker as a benzylic carbamate. Reduction of A16c gives A27c which after cyclisation gives intermediate A28c. A28c can perform an intramolecular 1,6-elimination releasing the quinone-methide species A25 as well as the carbamic acid species A30 which further eliminates carbon dioxide to give the free phenolic cargo A14. When L is the (1,4)-self-immolative linker
##STR00068##
and B is the amine-type cargo
##STR00069##
##STR00070##
[0324] In compounds of type A16d, L is a benzylic (1,4)-self-immolative linker and B is an amine-type cargo A.sup.2-NH-A.sup.3 which is connected to the linker as a benzylic carbamate. Reduction of A16d gives A27d which after cyclisation gives intermediate A28d. A29d can perform an intramolecular 1,4-elimination releasing the quinone-methide species A26 as well as the carbamic acid species A30 which further eliminates carbon dioxide to give the free phenolic cargo A14.
[0325] Without wishing to be bound by theory, it is assumed that the specific cyclic structure common to the presently claimed compounds can ensure that the dichalcogenide bond is stabilized against nonspecific reduction e.g. by cellular monothiols such as GSH under physiological conditions.
[0326] It is furthermore assumed that in some of the claimed compounds this stabilised dichalcogenide is reductively cleaved selectively by certain reductants, such as one or more oxidoreductases or redox effector proteins, and therefore that cargo release can be used as a quantification method for the activity of those reductants, which is a goal of probe development in diagnostics and in research. Such compounds are also useful in the development of candidate inhibitor drugs for those reductants, as the compound can be used to read out the degree of inhibition of the reductant that the candidate causes.
[0327] It is furthermore assumed that in some of the claimed compounds this stabilised dichalcogenide is reductively cleaved selectively in cells with dysregulated redox properties (including cells located in hypoxic environments, and cells with upregulated oxidoreductases) such as tumor cells and cells located in inflammatory microenvironments. Therefore, the effect of the agent that is released after reductive cleavage can be localized to pathological cells and environments which ensures that healthy cells and tissues are not exposed to similar levels of the agent. This pathology-specificity allows the presently claimed compounds which include a therapeutic or theranostic agent to be used as therapeutics for pathological cells and environments; and allows the presently claimed compounds which include a diagnostic or theranostic agent to be used as diagnostics for pathological cells and environments. Such selectivity is a longstanding goal of prodrug and diagnostic development, and offers a significant advantage compared to prior art dichalcogenide-reduction-triggered compound designs which are activated by typical cellular conditions without specificity for pathological cells (see e.g. Hyman, L. M. et al. Coordination Chemistry Reviews 2012, 256, 2333-2356. https://doi.org/10.1016/j.ccr.2012.03.009).
Utility, Diseases, and Examples
[0328] The compounds having the formula (I) are particularly useful as therapeutics or diagnostics.
[0329] In one embodiment, the present invention provides a (typically in vitro) method of determining (preferably quantifying) an inhibitory activity of a candidate inhibitor or candidate drug upon an oxidoreductase and/or a redox effector protein. The candidate inhibitor can be any compound which is to be tested to determine whether it can inhibit an oxidoreductase and/or a redox effector protein. The candidate drug is not particularly limited either. Examples of candidate drugs include any compound which is to be tested to determine whether it is suitable for treating, ameliorating, preventing or diagnosing a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury. Preferably the candidate drug is a compound which is assumed to be suitable for inhibiting an oxidoreductase and/or a redox effector protein.
[0330] In a first step, a compound having the formula (I), or a pharmaceutically acceptable salt, solvate, or ester thereof, wherein A.sup.1 is selected such that A.sup.1-OH is a diagnostic or theranostic agent or A.sup.2 and A.sup.3 are independently selected such that A.sup.2-NH-A.sup.3 is a diagnostic or theranostic agent, is contacted with the oxidoreductase and/or the redox effector protein as well as with the candidate inhibitor or candidate drug. The method of contacting the compound having the formula (I), or a pharmaceutically acceptable salt, solvate, or ester thereof, with the oxidoreductase and/or the redox effector protein as well as with the candidate inhibitor or candidate drug is not particularly limited. Typically the three components are mixed in a solvent such as water, typically with buffered pH, typically in the presence of a small amount of organic cosolvent (such as PBS, pH=7, containing 1% DMSO).
[0331] Subsequently, the sample is imaged according to the imaging modality of the diagnostic or theranostic agent, for instance, by fluorescence imaging or luminescence imaging, to determine whether the compound (I) of the present invention, or a pharmaceutically acceptable salt, solvate, or ester thereof, has been reductively cleaved to release A.sup.1-OH or A.sup.2-NH-A.sup.3. Depending on the imaging method, the detection can be quantitative or qualitative. In a preferred embodiment, A.sup.1-OH or A.sup.2-NH-A.sup.3 is a fluorescent agent which can be imaged by fluorescence imaging.
[0332] The more the compound of the present invention, or a pharmaceutically acceptable salt, solvate, or ester thereof, has been reductively cleaved the less the candidate inhibitor is suitable for inhibiting the oxidoreductase and/or the redox effector protein. The less the compound of the present invention, or a pharmaceutically acceptable salt, solvate, or ester thereof, has been reductively cleaved the more the candidate drug is suitable for treating the recited disorders and/or for inhibiting the oxidoreductase and/or the redox effector protein.
[0333] The extent to which the compound (I) of the present invention is reductively cleaved to release A.sup.1-OH or A.sup.2-NH-A.sup.3 is not particularly limited. For instance, if at most about 30%, preferably at most about 5% of the compound (I) of the present invention, or a pharmaceutically acceptable salt, solvate, or ester thereof, is reductively cleaved, then it can be assumed that the candidate inhibitor will be suitable as an inhibitor of the oxidoreductase and/or the redox effector protein. For example, if at most about 50%, preferably at most about 10% of the compound (I) of the present invention, or a pharmaceutically acceptable salt, solvate, or ester thereof, are reductively cleaved, then it can be assumed that the candidate drug will be suitable for treating, ameliorating, preventing or diagnosing the recited disorder or will be suitable as an inhibitor of the oxidoreductase and/or the redox effector protein.
[0334] The method of the above embodiment can be used as part of e.g. biochemical and biological research in diverse settings, wherein such compounds of the invention are valuable probes.
[0335] The compounds having the formula (I) can be used in the diagnosis, treatment, amelioration or prevention of a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury.
[0336] Preferably, the disorder is a neoplastic disorder, in particular cancer. The cancer is not particularly limited, preferably the cancer is selected from acoustic neuroma, adenocarcinoma, angiosarcoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, breast cancer, bronchogenic carcinoma, cervical cancer, chondrosarcoma, chordoma, choriocarcinoma, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliosarcoma, ependymoma, epithelial carcinoma, Ewing's tumor, fibrosarcoma, hemangioblastoma, leiomyosarcoma, liposarcoma, Merkel cell carcinoma, melanoma, mesothelioma, myelodysplastic syndrome, myxosarcoma, oligodendroglioma, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinomas, papillary carcinoma, pinealoma, prostate cancer, renal cell carcinoma, retinoblastoma, rhabdomyosarcoma, sebaceous gland carcinoma, seminoma, squamous cell carcinoma, sweat gland carcinoma, synovioma, testicular tumor, Wilms' tumor, adrenocortical carcinoma, urothelial carcinoma, gallbladder cancer, parathyroid cancer, Kaposi sarcoma, colon carcinoma, gastrointestinal stromal tumor, anal cancer, rectal cancer, small intestine cancer, brain tumor, glioma, glioblastoma, astrocytoma, neuroblastoma, medullary carcinoma, medulloblastoma, meningioma, leukemias including acute myeloid leukemia, multiple myeloma, acute lymphoblastic leukemia, liver cancer including hepatoma and hepatocellular carcinoma, lung carcinoma, non-small-cell lung cancer, small cell lung carcinoma, lymphangioendotheliosarcoma, lymphangiosarcoma, primary CNS lymphoma, non-Hodgkin lymphoma, and classical Hodgkin's lymphoma.
[0337] The therapeutic, diagnostic or theranostic compounds having the formula (I) can be used in combination with one or more other therapeutics, diagnostics or theranostics. The type of the other therapeutics, diagnostics or theranostics is not particularly limited and will depend on the disorder to be treated and/or diagnosed. Preferably, other therapeutics can enhance the selectivity and/or the turnover of the compound of formula (I) in the targeted tissue type; for example, the other therapeutic may be an agent that can stimulate transient hypoxia in tumors and thereby enhance the tumor-specific release of the cargo of the compound of formula (I) (which may be a therapeutic, diagnostic, or theranostic). Preferably in the case of compounds of formula (I) which are therapeutics and theranostics releasing a cargo used to treat a disorder, the other medicaments will be further therapeutics which are used to treat the same disorder; for example, further therapeutics that can treat a neoplastic disorder such as cancer may be applied in combination with compounds of formula (I) that are therapeutic proagents releasing a cargo that can treat a neoplastic disorder such as cancer.
[0338] The one or more other medicament, diagnostic or theranostic can be administered prior to, after, or simultaneously with the compound having formula (I). In another preferred embodiment, the other medicament can be an agent that can stimulate transient hypoxia in tumors which can be administered prior to, after or simultaneously to administration of compound of formula (I).
[0339] Agents which are capable of stimulating hypoxia in diseased tissues include but are not limited to vascular disrupting agents (including colchicine domain tubulin inhibitors such as combretastatin A-4 (CA4), 3-aminocombretastatin A-4, BNC105, ABT-751, ZD6126, combretastatin A-1, or prodrugs of the same (which includes but is not limited to combretastatin A-4 phosphate (CA4P), 3-aminocombretastatin A-4 3-serinamide (ombrabulin), combretastatin A-1 bisphosphate (CA1P), and BNC105 phosphate (BNC105P), and pharmaceutically acceptable salts of the same), vascular-acting drugs such as vasodilator or antihypertensive drugs (including hydralazine), and inhibitors of prolyl hydroxylase or stabilizers of hypoxia-inducible factor 1 such as daprodustat, desidustat and molidustat.
[0340] For in vivo usage, a diagnostic compound of the invention is administered to a subject or patient by any acceptable route, preferably i.v., i.p. or per os, and the subject or patient is imaged thereafter according to the imaging modality of the cargo, preferably by fluorescence imaging, photoacoustic imaging, luminescence imaging, or positron emission tomography. For usage in vitro, a diagnostic compound of the invention is administered to a test sample which, for instance, has been obtained from a subject, and the test sample is imaged thereafter according to the imaging modality of the cargo, preferably by fluorescence imaging, absorbance, or luminescence imaging; the sample may preferably be cells, a tissue section, a small animal model or a biopsy sample.
[0341] The compounds of the present invention can be used to predict the suitability of a compound of the invention for treating a patient who is suffering from a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury.
[0342] In a first step, a sample of the effected tissue or body fluid is obtained from the patient. The sample is then contacted with a compound (I*) of the present invention, wherein A is A*, L is L* and A.sup.1 is selected such that A.sup.1-OH is a diagnostic or theranostic agent or A.sup.2 and A.sup.3 are independently selected such that A.sup.2-NH-A.sup.3 is a diagnostic or theranostic agent. Subsequently, the sample is imaged according to the imaging modality of the diagnostic or theranostic agent, for instance, by fluorescence imaging, photoacoustic imaging, luminescence imaging, or positron emission tomography, to determine whether the compound (I*) of the present invention has been reductively cleaved to release A.sup.1-OH or A.sup.2-NH-A.sup.3. If compound of the present invention has been reductively cleaved then it can be assumed that a compound (I**) of the present invention which has the same moieties A* and L* as the compound (I*) but in which A.sup.1-OH is a therapeutic or theranostic agent or A.sup.2 and A.sup.3 are independently selected such that A.sup.2-NH-A.sup.3 is a therapeutic or theranostic agent will be effective for treating the patient. The theranostic agent which is used in the compounds (I*) and (I**) can be the same or different.
[0343] The extent to which the compound (I*) of the present invention is reductively cleaved to release A.sup.1-OH or A.sup.2-NH-A.sup.3 is not particularly limited. It should be sufficient to provide a therapeutically effective amount of A.sup.1-OH or A.sup.2-NH-A.sup.3 to the tissue or body fluid. For instance, 0.1-100%, preferably 0.5-50% of the compound (I*) of the present invention can be reductively cleaved.
[0344] In the following, examples of preferred compounds having the formula (I) are described, to illustrate some of the ways in which conjugation of a cargo H-B as the compound A-L-B masks its diagnostic and/or therapeutic activity, until the cargo is released following reduction.
[0345] These examples include the following therapeutic designs:
[0346] 1 releases a DNA-alkylating nitrogen mustard cargo subunit of a type which is of interest especially for cancer therapy. The released bis(2-haloethyl)aniline species will feature similar activity to PR-104's bioactive alkylating metabolite PR-104M whereas the intact species 1 will not be as potently alkylating as the released cargo, since unmasking the electron-donating phenol in para to the aniline nitrogen increases electronic density and alkylating reactivity (similar principles are known for PR-104 and a range of related prodrugs; Sharma, A. et al. Chem. Soc. Rev. 2019, 48, 771-813. https://doi.org/10.1039/C8CS00304A). Note that a range of leaving groups (halogens Cl, Br, I or other leaving groups such as OS(O).sub.2CH.sub.3) can be used as the halogen substituents of the 2-haloethyl groups without compromising functionality, and/or an aniline nitrogen (requiring the use of a spacer subunit) could be used instead of the phenolic hydroxyl as the unmasked group of the cargo, while remaining within the scope of a compound having the formula (I).
[0347] 4 and 9 are similar to compound P8 in that they release cargo subunits that are analogues of 5-hydroxy-seco-cyclopropabenzaindole, which (when their phenolic hydroxyl is unmasked) can then undergo Winstein cyclisation to the active DNA-alkylating cyclopropabenzaindoles (CBIs), which are of interest especially for cancer therapy, whereas no Winstein cyclisation can take place until the phenol is unmasked. In this way only the released cargo (and not the proagents) will be bioactive (similar principles are known for a range of related prodrugs; Twum, E. A. et al. Bioorganic & Medicinal Chemistry 2015, 23, 3481-3489. https://doi.org/10.1016/j.bmc.2015.04.034). Note that a range of analogues of 5-hydroxy- or 5-amino-seco-cyclopropabenzaindoles or related cargos with various substitution motifs (such as the inclusion of the dimethylamino substituent on 9 to enhance solubility and DNA-binding potency) could be used, while remaining within the scope of a compound having the formula (I).
[0348] 11 releases a cargo subunit that is the Amaryllidaceae-type alkaloid pancratistatin. Amaryllidaceae-type alkaloids (such as pancratistatin or narciclasine) are metabolism-affecting drugs that have shown utility in disease indications featuring metabolic deregulation, from cancer to chronic inflammation (Lubahn, C. et al. Rheumatology International 2012, 32, 3751-3760. https://doi.org/10.1007/s00296-011-2217-z; McNulty, J. et al. J. Nat. Prod. 2008, 71, 357-363. https://doi.org/10.1021/np0705460). The cargo release process (following triggering of the dichalcogenide trigger subunit of 11) removes steric hindrance and so allows binding of the lycorane to its molecular target, such that only the released cargo is bioactive. Note that a range of lycorane-type alkaloids with various substitution motifs could be used, while remaining within the scope of a compound having the formula (I).
[0349] 14 releases a cargo subunit that is SN-38, the active metabolite of the camptothecin class topoisomerase inhibitor irinotecan, a class which also contains the agent topotecan. As known for irinotecan, blocking the phenol removes bioactivity of the proagent so 14 will be biologically inactive except if triggered to release cargo; note that a range of similar cargo structures (e.g. modifying the ethyl group at the 7-position, or introduction of solubility and potency enhancing groups such as (dimethylamino)methyl as seen in topotecan) could be used, as is known for a range of related prodrugs (Sharma, A. et al. Chem. Soc. Rev. 2019, 48, 771-813. https://doi.org/10.1039/C8CS00304A), while remaining within the scope of a compound having the formula (I).
[0350] It is illustrated by 1, 4, 9, 11 and 14 that a broad range of bioactive phenolic cargos with a range of different bioactivity mechanisms and disease indication scopes can therefore be used, including with extensive structural substitutions, within the scope of the invention to deliver cargo-release-based activation of proagent bioactivity, i.e. functional prodrugs dependent upon triggering of the key selenenylsulfide of a compound having the formula (I).
[0351] 13 releases an amine cargo subunit that is a tubulin-inhibiting auristatin derivative, of particular interest for cancer therapy and for neoangiogenic vasculopathies. A range of auristatins (and related peptide tubulin binders such as dolastatins and tubulysins) have been used in prodrugs where bioactivity is suppressed by conjugation of the agent to a large but cleavable trigger and/or trigger+spacer subunit (Singh, Y. et al. Curr Med Chem 2008, 15, 1802-1826), then restored upon its removal. Note that a range of such agents with various substitution patterns (including monomethyl auristatin E, monomethyl auristatin F, dolastatin or symplostatin) could be used; and/or that beyond the 3-fluoro-4-hydroxybenzyl alcohol spacer depicted in 13, a range of spacers could instead be used, while remaining within the scope of a compound having the formula (I), to activate cytotoxic activity upon triggering of the proagent.
[0352] 15 releases an amine cargo subunit that is of the anthracycline class of topoisomerase-inhibitors. This class includes doxorubicin, daunorubicin and idarubicin, and is of particular interest for cancer therapy. Basicity of the aliphatic amine of doxorubicin and its analogues is important for bioactivity; its carbamylation in 15 blocks bioactivity, but bioactivity can be restored by diselenide triggering followed by spacer-mediated release of the doxorubicin analogue (Singh, Y. et al. Curr Med Chem 2008, 15, 1802-1826). Note that a range of related cargos with various substitution motifs and/or related spacers could instead be used, while remaining within the scope of a compound having the formula (I), to activate cytotoxic activity upon triggering of the proagent.
[0353] It is illustrated by 13 and 15 that a broad range of bioactive amine cargos with different bioactivity mechanisms and disease indication scopes can therefore be used in conjunction with a range of spacers, each with optional extensive structural substitutions, while remaining within the scope of the invention to deliver cargo-release-based activation of proagent bioactivity, i.e. functional prodrugs dependent upon triggering of the key dichalcogenide of a compound having the formula (I).
[0354] These examples include the following diagnostic designs:
[0355] 2 releases a fluorescent precipitating phenolic cargo subunit with large Stokes shift and green emission; 3, 7 and 10 release fluorescent phenolic cargos with small Stokes shifts and UV, cyan, or orange emission; all the proagents 2, 3, 7 and 10 are nonfluorescent due to masking of the phenol groups (either due to blocking excited-state intramolecular proton transfer in 2, or reducing electron-donating capacity in 3, or blocking the opening of the spirolactone in 7 and 10); and all these designs provide a signal turn-on upon cargo release stimulated by triggering of the trigger subunits (and similar principles are known for a range of related probe cargos; Thorn-Seshold, O. et al. Chem. Commun. 2012, 48, 6253-6255. https://doi.org/10.1039/C2CC32227G); they are thus diagnostic fluorescence probes that may be useful in a variety of diagnostic purposes. It is also illustrated that spacer subunits can be optionally included (7), and that a range of cargos [e.g. 2 (2-hydroxyphenyl)quinazolin-4(3H)-one), 3 (coumarin), 7 and 10 (xanthene fluorophores of the fluorescein type)] with a range of structures and diagnostic properties are possible while remaining within the scope of a compound having the formula (I).
[0356] 5 releases a phenolic cargo subunit that is D-luciferin, the substrate of firefly luciferase enzyme, whereas the intact probe 5 is too bulky to be a substrate of luciferase. Therefore 5 is a dichalcogenide-reduction-triggered diagnostic luminescence probe allowing detection by bioluminescence in the presence of luciferase, which may be useful as a diagnostic for reductive probe turnover with ultra-low background signal (high sensitivity). Note that a range of luciferins for different luciferases and with different structural modifications are known and could also be used as cargo subunits (Kaskova, Z. M. et al. Chem. Soc. Rev. 2016, 45, 6048-6077. https://doi.org/10.1039/C6CS00296J) while remaining within the scope of a compound having the formula (I).
[0357] 8 releases a phenolic cargo subunit that is a functional analogue of Black Hole Quencher 3 (BHQ3) which has fluorescence quenching and photoacoustic signal generation properties (Chevalier, A. et al. Chem.-Eur. J 2013, 19, 1686-1699. https://doi.org/10.1002/chem.201203427). The proagent 8 is non-signal-generating and non-quenching due to masking of the key electron-donating phenol group, while the released cargo has no masking of the phenol and therefore has fluorescence quenching and photoacoustic signal generation activity, thus 8 functions as a multimodal diagnostic proagent. Note that similar unmasking-based activity turn-on principles are known for a range of related compounds including other quenchers, which could also be used as cargo subunits while remaining within the scope of a compound having the formula (I).
[0358] 12 releases a spacer-cargo system that fragments to release an aniline cargo subunit that is a silarhodamine derivative which has fluorescence and photoacoustic signal generation properties (Myochin, T. et al. J. Am. Chem. Soc. 2015, 137, 4759-4765. https://doi.org/10.1021/jacs.5b00246). The proagent 12 is non-signal-generating and nonfluorescent due to the masking of the amine group by carbamylation, while the released cargo silarhodamine is active in both diagnostic modalities. Note that a range of related or analogous cargos with various substitution motifs (such as a range of aniline-based fluorophores or photoacoustics dyes, including rhodols and rhodamines) could also be used as cargo subunits along the same design principles while remaining within the scope of a compound having the formula (I).
[0359] It is illustrated by 2, 3, 5, 7, 8, 10 and 12 that a broad range of diagnostically active phenol or amine cargos, eabling a range of structures and diagnostic detection methods, can therefore be used, including with extensive structural substitutions, within the scope of the invention, to deliver cargo-release-based activation of proagent diagnostic activity, i.e. functional diagnostic probes dependent upon triggering of the key dichalcogenide of a compound having the formula (I).
[0360] These examples also include the theranostic design 6 that releases a cargo subunit that is leuco-methylene blue, a reduced form of the theranostic compound methylene blue which after release in biological media is spontaneously converted to methylene blue (similar principles are known for a range of related prodrugs). Methylene blue can be useful therapeutically including as a photosensitiser in photodynamic therapy (e.g. of the autoimmune disorder psoriasis, or of cancers) and/or as a redox-active selective toxin (e.g. against malaria parasite); as well as diagnostically (e.g. as a fluorescent agent or a photoacoustic dye); therefore being capable of theranostic activity (Schirmer, R. H. et al. Neurobiology of Aging 2011, 32, 2325.e7-2325.e16. https://doi.org/10.1016/j.neurobiolaging.2010.12.012). Note that a range of functionally and structurally related compounds (e.g. phenoxazines) can also be used as cargo subunits in theranostics following the same design principles while remaining within the scope of a compound having the formula (I).
[0361] Various modifications and variations of the invention will be apparent to those skilled in the art without departing from the scope of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention which are obvious to those skilled in the relevant fields are intended to be covered by the present invention.
Examples
[0362] The invention will be more fully understood by reference to the following examples. They should not, however, be construed as limiting the scope of the invention.
1. Abbreviations
[0363] A549: human lung cancer cell line A549 [0364] aq.: aqueous (solution) [0365] 4T1: Balb/c mouse breast cancer cell line [0366] Boc: tert-butoxycarbonyl [0367] calc: calculated [0368] DCM: dichloromethane [0369] DIAD: DIAD [0370] DMF: dimethylformamide [0371] DMSO: dimethylsulfoxide [0372] EDCI: 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide [0373] EI: electron ionization [0374] ESI: electron spray ionization [0375] FCC: flash column chromatography [0376] HeLa: Henrietta Lacks, human cervical cancer cell line [0377] (HP)LC: (high performance) liquid chromatography [0378] HRMS: high-resolution mass spectrometry [0379] DIPEA diisopropylethylamine [0380] J: coupling constant [0381] LC-MS: liquid chromatography mass spectrometry [0382] LRMS: low-resolution mass spectrometry [0383] MEF: mouse embryonic fibroblast cell line [0384] MF-OH: 3-hydroxy-6-methoxy-3H-spiro[isobenzofuran-1,9-xanthen]-3-one [0385] NMR: nuclear magnetic resonance [0386] Pd/C: palladium on charcoal, 10% wt [0387] (?) ppm: chemical shift (parts per million) [0388] PMB: p-methoxy benzyl [0389] PQ-OH: 6-chloro-2-(5-chloro-2-hydroxyphenyl)quinazolin-4(3H)-one [0390] p-TSA: p-toluenesulfonic acid [0391] R.sub.f: retention factor [0392] RP: reversed phase [0393] r.t.: room temperature [0394] sat. saturated (solution) [0395] THF: tetrahydrofuran [0396] TLC: thin layer chromatography
2. General Synthesis Conditions
[0397] Unless stated otherwise, (1) all reactions and characterisations were performed with solvents and reagents, used as obtained, under closed air atmosphere without special precautions; (2) DCM, Et.sub.2O, THF and DMF used for synthesis were distilled, then dried on activated molecular sieves; (3) isohexane and EtOAc used for chromatography was distilled from commercial crude iso-hexane fraction; (4) column and chromatography refer to FCC, performed on Merck silica gel Si-60 (40-63 ?m); (5) procedures and yields are unoptimised; (6) yields refer to isolated chromatographically and spectroscopically pure materials, corrected for residual solvent content.
[0398] NMR: Standard NMR characterisation was carried out by .sup.1H- and .sup.13C-NMR spectra. Avance III Bruker BioSpin 400 MHz, 500 MHz and 600 MHz spectrometers were used (.sup.1H: 400 MHz, 500 MHz or 600 MHz, .sup.13C: 101 MHz, 126 MHz and 151 MHz respectively) typically at 298 K, although with some carbamates spectroscopy was simplified by measuring at 373 K to induce a dynamic equilibrium between their rotameric species. Chemical shifts (?) are reported in ppm calibrated to residual non-perdeuterated solvent as an internal reference. Peak descriptions singlet (s), doublet (d), triplet (t), quartet (q), pentuplet (p), and multiplet (m) are used. Coupling constants J are given in Hz.
[0399] Mass spectra: Unit mass measurements were performed on an AGILENT 1200 SL coupled LC-MS system with ESI mode ionisation, with binary eluent mixtures of H.sub.2O:MeCN, with the water containing formic acid. HRMS was carried out by the Zentrale Analytik of the Ludwig-Maximilian-Univeristy (LMU), Munich (electron impact (EI) at 70 eV with a Thermo Finnigan MAT 95 or a Jeol GCmate II spectrometer; electrospray ionization (ESI) with a Thermo Finnigan LTQ FT Ultra Fourier Transform Ion Cyclotron resonance mass spectrometer) in positive or negative mode as stated.
3. General Synthesis Protocols
General Procedure A: Selenocyanation of Alkyl Mesylates
[0400] The mesylate was dissolved in THF (0.1 M) and NaI (3.0 equiv.) was added. After 5 min, 18-crown-6 (1.1 equiv.) and KSeCN (2.2 equiv. per mesylate) were sequentially added and the colourless reaction mixture was heated to 50? C. for 16 h. The reaction mixture was diluted with brine and DCM, the organic layer was removed, and the aqueous layers were extracted with DCM (three times). The combined organic phases were dried over MgSO.sub.4, filtered and concentrated in vacuo. Column chromatography allowed purification of the orange crude to afford alkyl selenocyanates as pale yellow solids.
General Procedure B: Base-Mediated Selenenylsulfide or Diselenide Formation
[0401] A solution of (thioacetate) (selenocyanate) or bis(selenocyanate) starting material (1.0 equiv.) in THF (0.05 M), was charged with a methanolic solution of KOH (0.2 M, 1.4 equiv.) in a dropwise manner. When TLC control indicated full conversion of the starting material (typically within minutes) the reaction was quenched with NaHCO.sub.3 (sat. aq.), and the aqueous layer was extracted with DCM (three times). The combined organic layers were dried over MgSO.sub.4, filtered and concentrated in vacuo. The targeted selenenylsulfide or diselenide (respectively) was received as a deeply coloured solid and used without further purification.
General Procedure C: Methylation of N-Boc Protected Amines
[0402] In a flame-dried Schlenk flask, N-Boc protected primary amine (1.0 equiv.) was dissolved in dry DMF (20 mM) under nitrogen atmosphere. The solution was cooled to 0? C. and first Mel (1.1 equiv.) then NaH (60%, 1.1 equiv.) were added. The reaction mixture was allowed to slowly warm to r.t. and was stirred for 5 to 7 h, upon which TLC control confirmed full conversion of the starting material. The reaction was quenched with NaHCO.sub.3 (aq. sat.) and diluted with DCM. The aqueous layer was extracted with DCM (three times) and the combined organic layers were dried over MgSO.sub.4 and concentrated under reduced pressure. The crude product was purified by column chromatography to yield the target species as colourless or yellow oil.
General Procedure D: Monoalkylation of Primary Amines by 3-Bromo-Propionyl Derivatives
[0403] Step 1: To a solution of the primary amine (1.5 equiv.) in MeOH (0.3 M) was added the respective N-substituted 3-bromo-propenamide (1.0 eq). In case the starting amine was used as an HCl salt, NEt.sub.3 (1.5 equiv.) was added for neutralisation. The clear solution was heated in a sealed tube using a laboratory microwave (120? C., 25 W, 60 min), was then cooled to r.t. and concentrated under reduced pressure.
[0404] Step 2: The material obtained in step 1 was suspended in DCM (0.1 M) and charged with triethylamine (3.0 equiv.) and Boc.sub.2O (1.8 equiv., 1 M in anhydrous DCM) at r.t., and the mixture was stirred at retained temperature for 15 h. The reaction was then concentrated under reduced pressure. Purification by flash column chromatography yielded the N-Boc protected alkylation product of the primary amine.
General Procedure E: Boc-Deprotection of Primary Amines
[0405] Boc-protected amine (1.0 equiv.) was dissolved in DCM (0.1 M), and HCl (4 M in dioxane, 10 equiv.) was added at r.t. in a dropwise manner. In most cases and within minutes, formation of a precipitate was observed. Full conversion of the starting material was checked by TLC and observed within 4-12 hours. Excess HCl and DCM were removed under reduced pressure, yielding targeted amines as HCl salts. Caution: to remove HCl, use a leak-proof rotary evaporator in a well-ventilated hood.
General Procedure F: Carbamate Formation with Primary Amine Hydrochlorides and PQ-Chloroformate
[0406] A trigger-amine hydrocholoride was suspended in DCM (50 mM) and solubilised by adding NEt.sub.3 (1.1-2.2 equiv.), regenerating the free amine. Subsequently, to a suspension of preformed PQ-OC(O)Cl X22 in DCM (0.04 M) was slowly added the trigger amine and the resulting reaction mixture was stirred for 1 h. Once LCMS-control indicated full conversion of the starting material, the reaction mixture was concentrated in vacuo yielding a pale yellow crude oil. In most cases, flash chromatography provided the target carbamates in excellent purity and ensured no residual fluorescence of unreacted fluorophore, though additional purification by prepHPLC could be conducted as needed.
4. Synthesis
Precursors to Proagents
N-Boc-dimethylaspartate (X1)
[0407] ##STR00071##
[0408] A methanolic suspension (0.3 M, 200 mL) of aspartic acid (10.0 g, 75.1 mmol, 1.0 equiv.) was reacted with SOCl.sub.2 (21.8 mL, 301 mmol, 4.0 equiv.) and after evaporation of the solvent and gaseous byproducts, dimethylaspartate hydrochloride was received as a colourless solid. Without further purification, the obtained dimethyl ester was dissolved in a 1:1-mixture of water (75 mL) and dioxane (75 mL), cooled to 0? C. and was charged with NEt.sub.3 (26.0 mL, 188 mmol, 2.5 equiv.) and Boc.sub.2O (19.7 g, 90.1 mmol, 1.2 equiv.). The reaction mixture was stirred for 14 h, acidified and extracted to yield a colourless crude product. Purification was achieved running a short column (SiO.sub.2, h=3 cm, d=6 cm, hexanes/EtOAc 3:1, 50 mL tubes, Fr8-20) giving target species 39 as a colourless solid (18.7 g, 71.6 mmol, 95%).
[0409] TLC R.sub.f=0.39 (hexanes/EtOAc 3:1). .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=5.48 (d, J=8.7 Hz, 1H), 4.57 (dt, J=9.2, 4.7 Hz, 1H), 3.75 (s, 3H), 3.69 (s, 3H), 2.99 (dd, J=16.9, 4.7 Hz, 1H), 2.81 (dd, J=16.9, 4.7 Hz, 1H), 1.44 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=171.7, 171.5, 155.5, 80.3, 52.8, 52.1, 50.0, 36.8, 28.4. HRMS (EI.sup.+): m/z calc. for C.sub.1H.sub.19NO.sub.6 [M].sup.+ 261.1207, found 261.1206.
N-Boc-2-aminobutane-1,4-diol (X2)
[0410] ##STR00072##
[0411] Diester X1 (7.00 g, 26.8 mmol, 1.0 equiv.) in anhydrous THE (0.2 M, 135 mL) was carefully added to a suspension of LAH (3.05 g, 80.4 mmol, 3.0 equiv.) in dry THF (0.2 M, 135 mL) at 0? C. After 3 h, Fieser-type work up was conducted giving a colourless crude oil. Flash chromatography (hexanes/EtOAc 1:1.fwdarw.EtOAc) ultimately provided the target diol as a colourless solid (4.00 g, 19.5 mmol, 73%). NMR spectra of X2 match reported data. (Lukesh; J. Am. Chem. Soc. 2012 134, 4057-4059)
[0412] TLC R.sub.f=0.30 (EtOAc). .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.99 (s, 1H), 3.91-3.82 (m, 1H), 3.78-3.62 (m, 4H), 2.24 (s, 2H), 1.81 (ddt, J=14.1, 9.3, 4.6 Hz, 1H), 1.63 (tt, J=10.1, 4.2 Hz, 1H), 1.45 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=157.2, 80.2, 65.6, 58.9, 49.5, 35.0, 28.5. HRMS (EI.sup.+): m/z calc. for CH.sub.16NO.sub.3 [M-CH.sub.3O].sup.+ 174.1125, found 174.1122.
N-Boc-2-aminobutane-1,4-diyl dimethanesulfonate (X3)
[0413] ##STR00073##
[0414] A solution of diol X2 (0.18 g, 0.87 mmol, 1.0 equiv.) in anhydrous DCM (0.05 M, 20 mL) was charged with NEt.sub.3 (0.61 mL, 4.3 mmol, 5.0 equiv.) and MsCl (0.15 mL, 2.0 mmol, 2.3 equiv.) at 0? C. Acidic workup after 3 h and purification by column chromatography (hexanes/EtOAc 1:1.fwdarw.hexanes/EtOAc 1:2) gave X3 as a colourless solid (0.30 g, 0.82 mmol, 94%).
[0415] TLC R.sub.f=0.37 (hexanes/EtOAc 1:2) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.78 (d, J=8.7 Hz, 1H), 4.39-4.23 (m, 4H), 4.10-4.02 (m, 1H), 3.05 (s, 3H), 3.04 (s, 3H), 2.11-2.04 (m, 1H), 2.02-1.93 (m, 1H), 1.45 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=155.3, 80.5, 71.0, 66.3, 46.9, 37.5 (2C), 31.0, 28.4 (3C). HRMS (EI.sup.+): m/z calc. for C.sub.11H.sub.23NO.sub.8S.sub.2[M].sup.+ 361.0860, found 361.0861.
N-Boc-(3-amino-4-(methanesulfonyl)oxybutyl)ethanethioate (X4) & N-Boc-(2-amino-4-(methanesulfonyl)oxybutyl)ethanethioate X5
[0416] ##STR00074##
[0417] 18-crown-6 (0.12 g, 0.33 mmol, 1.2 equiv.) and KSAc (32 mg, 0.277 mmol, 1.0 equiv.) were sequentially added to a solution of X3 (0.10 g, 0.28 mmol, 1.0 equiv.) in dry DMF (0.05 M, 27 mL). After 15 h, the solvent was removed and the obtained crude was purified by column chromatography (hexanes/EtOAc 1:1) yielding X4 as a colourless solid (55 mg, 0.16 mmol, 57%) along with its regioisomeric form X5 (21 mg, 87 ?mol, 31%).
[0418] X4: TLC R.sub.f=0.40 (hexanes/EtOAc 1:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.75 (s, 1H), 4.31-4.20 (m, 2H), 3.91 (br s, 1H), 3.04 (s, 3H), 3.03-2.95 (m, 1H), 2.89-2.81 (m, 1H), 2.34 (s, 3H), 1.85 (dt, J=13.7, 6.9 Hz, 2H), 1.45 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=195.8, 155.4, 71.0, 49.1, 37.5, 31.6, 30.8, 28.5, 25.6. HRMS (EI.sup.+): m/z calc. for C.sub.12H.sub.23NO.sub.6S.sub.2 [M].sup.+ 341.0961, found 341.0957.
[0419] X5: TLC R.sub.f=0.44 (hexanes/EtOAc 1:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.59 (d, J=8.9 Hz, 1H), 4.33-4.25 (m, 2H), 3.90 (s, 1H), 3.08 (dd, J=6.2, 2.9 Hz, 2H), 3.04 (s, 3H), 2.37 (s, 3H), 2.07-1.99 (m, 1H), 1.90-1.80 (m, 1H), 1.43 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=195.7, 155.6, 66.9, 47.9, 37.5, 33.8, 30.7, 28.5. HRMS (EI.sup.+): m/z calc. for C.sub.12H.sub.23NO.sub.6S.sub.2 [M].sup.+ 341.0961, found 341.0930.
N-Boc-(2-amino-4-selenocyanatobutyl)ethanethioate (X6)
[0420] ##STR00075##
[0421] Selenocyanation was conducted based on general procedure A. Mesylate X4 (0.60 g, 1.8 mmol, 1.0 equiv.) was dissolved in THE (0.1 M, 20 mL), subjected to NaI (0.79 mg, 5.3 mmol, 3.0 equiv.), 18-crown-6 (5.1 g, 1.9 mmol, 1.1 equiv.) and KSeCN (557 mg, 3.87 mmol, 2.2 equiv.), and the resulting mixture was heated to 50? C. for 10 h. Purification via column chromatography (hexanes/EtOAc 4:1.fwdarw.hexanes/EtOAc 3:1) followed aqueous work up and ultimately provided X6 as a pale yellow solid (552 mg, 1.57 mmol, 89%). Note: X6 proved to be rather unstable being stored at ambient temperature and on air and turned purple within a few weeks.
[0422] TLC R.sub.f=0.20 (hexanes/EtOAc 3:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.82 (s, 1H), 3.88 (h, J=7.8, 7.3, 6.3, 6.3, 5.1 Hz, 1H), 3.44-3.31 (m, 1H), 3.31-3.22 (m, 1H), 3.04-2.97 (m, 1H), 2.85 (dt, J=13.9, 7.7 Hz, 1H), 2.35 (s, 3H), 1.88 (q, J=7.3 Hz, 2H), 1.46 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=196.0, 155.5, 80.6, 50.3, 41.0, 35.1, 34.2, 30.8, 28.5, 25.7. LRMS (ESI+): m/z calc. for C.sub.11H.sub.18NO.sub.3SSe [M-CH.sub.2N].sup.+ 325.02, found 324.99.
N-Boc-(2-amino-5-selenocyanatobutyl)ethanethioate (X7)
[0423] ##STR00076##
[0424] Following selenocyanation protocol A, mesylate X5 (0.44 g, 1.3 mmol, 1.0 equiv.) was dissolved in THF (0.1 M, 13 mL) and subjected to NaI (0.58 g, 3.9 mmol, 3.0 equiv.), 18-crown-6 (0.38 g, 1.4 mmol, 1.1 equiv.) and KSeCN (0.41 g, 2.8 mmol, 2.2 equiv.) in a consecutive manner. After having stirred the resulting, colourless suspension at 50? C. for 10 h, aqueous work up gave an orange crude product which was purified by column (hexanes/EtOAc 3:1). X7 was received as a pale yellow solid (0.43 g, 1.2 mmol, 92%).
[0425] TLC R.sub.f=0.55 (hexanes/EtOAc 1:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.60 (d, J=9.0 Hz, 1H), 3.91 (s, 1H), 3.19 (ddd, J=12.6, 7.8, 5.1 Hz, 1H), 3.07 (d, J=6.2 Hz, 2H), 2.99 (dt, J=12.3, 7.9 Hz, 1H), 2.38 (s, 3H), 2.18-2.07 (m, 1H), 2.06-1.97 (m, 1H), 1.43 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=195.8, 156.0, 80.3, 50.5, 41.0, 36.4, 33.6, 30.7, 28.5, 26.6. HRMS (EI.sup.+): m/z calc. for C.sub.12H.sub.20N.sub.2O.sub.3SSe [M].sup.+ 352.0354, found 352.0352.
N-Boc-1,4-diseienocyanatobutan-2-amine (X8)
[0426] ##STR00077##
[0427] NaI (83 mg, 0.55 mmol, 1.0 equiv.), 18-crown-6 (0.32 g, 1.2 mmol, 2.2 equiv.) and KSeCN (0.20 g, 1.4 mmol, 2.5 equiv.) were added to a THF-solution (0.1 M, 6 mL) of dimesylate X3 (0.20 g, 0.55 mmol, 1.0 equiv.). After heating for 20 h, aqueous work up and flash chromatography (hexanes/EtOAc 3:1.fwdarw.hexanes/EtOAc 1:1) gave the title compound as a colourless solid (0.18 g, 0.46 mmol, 83%).
[0428] TLC R.sup.f=0.50 (hexanes/EtOAc 1:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.74 (s, 1H), 4.06 (h, J=7.5, 6.6 Hz, 1H), 3.43-3.13 (m, 3H), 3.01 (dt, J=12.4, 7.8 Hz, 1H), 2.31-2.11 (m, 2H), 1.45 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=155.5, 101.5, 101.4, 81.0, 50.7, 35.8, 34.5, 28.4, 25.8. HRMS (EI.sup.+): m/z calc. for C.sub.10H.sub.16N.sub.2O.sub.2Se.sub.2 [M-CH.sub.2N].sup.+ 355.9537, found 355.9537.
N-Boc-1,2-thiaselenan-4-amine (X9)
[0429] ##STR00078##
[0430] Thiaselenane-formation was achieved according to general procedure B. X6 (0.70 g, 2.0 mmol, 1.0 equiv.) was dissolved in THF (0.05 M, 40 mL) and KOH was added (0.17 M in MeOH, 16 mL, 2.7 mmol, 1.4 equiv.). Within seconds, the colourless reaction mixture turned yellow and TLC control indicated full conversion of the starting material. Aqueous work up gave pure X9 as a pale yellow solid (0.55 mg, 2.0 mmol, 100%).
[0431] TLC R, =0.61 (hexanes/EtOAc 3:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.93 (s, 1H), 3.94 (s, 1H), 3.23 (ddd, J=14.3, 7.8, 2.8 Hz, 2H), 3.00 (d, J=13.2 Hz, 1H), 2.87-2.78 (m, 1H), 2.16 (d, J=13.1 Hz, 1H), 1.77 (s, 1H), 1.45 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=154.7, 80.0, 47.4, 35.1, 32.2, 30.1, 28.5. HRMS (EI+): m/z calc. for C.sub.9H.sub.17NO.sub.2SSe [M].sup.+ 283.0140, found 283.0136.
N-Boc-1,2-thiaselenan-5-amine (X10)
[0432] ##STR00079##
[0433] According to general procedure B, X7 (0.40 g, 1.1 mmol, 1.0 equiv.) was dissolved in THF (0.05 M, 20 mL) and potassium hydroxide (0.17 M in MeOH, 10 mL, 1.7 mmol, 1.5 equiv.) was slowly added. The reaction mixture immediately changed from colourless to yellow and, within 5 min, full conversion of the starting material was indicated by TLC. Aqueous workup and concentration of the combined organic extracts gave the desired product as a pale yellow solid (0.32 g, 1.1 mmol, 100%).
[0434] TLC R.sub.f=0.64 (hexanes/EtOAc 3:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=5.02 (s, 1H), 3.96-3.83 (m, 1H), 3.30-3.13 (m, 2H), 2.97 (s, 1H), 2.80 (dd, J=13.5, 7.7 Hz, 1H), 2.25 (t, J=11.8 Hz, 1H), 2.04 (s, 1H), 1.45 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=154.8, 79.9, 47.2, 37.7, 28.5, 23.1. HRMS (EI.sup.+): m/z calc. for C.sub.9H.sub.17NO.sub.2SSe [M].sup.+ 283.0140, found 283.0133.
N-Boc-1,2-diselenan-4-amine (X11)
[0435] ##STR00080##
[0436] Diselenide formation was achieved in analogy to general protocol B: di-selenocyanate X8 (0.50 g, 1.3 mmol, 1.0 equiv.) was provided in THF-solution (0.03 M, 30 mL) and KOH (0.17 M in MeOH, 9.4 mL, 1.6 mmol, 1.2 equiv.) was added. Within seconds, a deeply yellow species formed in the reaction vessel and full conversion was achieved according to TLC control. Subsequently conducted aqueous work up provided the title compound as a yellow solid (0.35 mg, 1.1 mmol, 85%).
[0437] TLC R.sub.f=0.58 (hexanes/EtOAc 3:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=5.03 (s, 1H), 4.00-3.81 (m, 1H), 3.30 (tdd, J=15.4, 9.1, 2.0 Hz, 2H), 3.10-3.00 (m, 1H), 2.89 (dd, J=12.3, 8.2 Hz, 1H), 2.22 (ddt, J=14.2, 8.8, 2.9 Hz, 1H), 2.03-1.91 (m, 1H), 1.43 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=154.6, 79.8, 47.5, 35.0, 28.5, 28.5, 22.2. HRMS (EI.sup.+): m/z calc. for C.sub.9H.sub.17NO.sub.2Se.sub.2 [M].sup.+ 330.9584, found 330.9588.
N-Boc-N-methyl-1,2-thiaselenan-4-amine (X12)
[0438] ##STR00081##
[0439] Following the general protocol C, Mel (79 ?L, 1.3 mmol, 1.2 equiv.) then NaH (47 mg, 1.2 mmol, 1.1 equiv.) were added to a solution of X9 (0.30 g, 1.1 mmol, 1.0 equiv.) in dry DMF (0.02 M, 60 mL). After 5 h, basic, aqueous workup was followed by flash chromatography (hexanes/EtOAc 93:7) yielding X12 as a pale yellow oil (88 mg, 0.30 mmol, 28%). Note: the title compound was observed as a mixture of rotameric species. Reported NMR data refer to the major rotamer.
[0440] TLC R.sub.f=0.30 (hexanes/EtOAc 93:7) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.42 (s, 1H), 3.37-3.15 (m, 3H), 2.75 (s, 3H), 2.69 (ddd, J=11.7, 3.1, 1.3 Hz, 1H), 2.09 (d, J=13.2 Hz, 1H), 1.95 (m, 1H), 1.46 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=155.2, 80.1, 54.4, 36.3, 34.0, 29.0, 28.6 (3C), 27.0. HRMS (ESI?): m/z calc. for C.sub.10H.sub.13NO.sub.2SSe [M?H].sup.? 296.0229, found 296.0230.
N-Boc-N-Methyl-1,2-thiaselenan-5-amine (X13)
[0441] ##STR00082##
[0442] Methylation of 1,2-thiaselenan X10 was achieved following general procedure C: The starting material (0.20 g, 0.71 mmol, 1.0 equiv.) was dissolved in dry DMF (0.03 M, 25 mL) and charged with Mel (53 ?L, 0.85 mmol, 1.2 equiv.) and NaH (60%, 31 mg, 0.78 mmol, 1.1 equiv.) at 0? C. After 5 h, aqueous work up was conducted and the obtained crude was purified by column chromatography (hexanes/EtOAc 93:7) yielding X13 as a yellow oil (0.14 g, 0.48 mmol, 68%). Note: the title compound was observed as a mixture of rotameric species. Reported NMR data refer to the major rotamer.
[0443] TLC R.sub.f=0.30 (hexanes/EtOAc 9:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.17 (s, 1H), 3.42 (t, J=12.3 Hz, 1H), 3.14 (t, J=12.5, 10.6 Hz, 1H), 3.04 (td, J=12.8, 3.2 Hz, 1H), 2.79 (d, J=3.0 Hz, 1H), 2.76 (s, 3H), 2.15 (d, J=26.2 Hz, 2H), 1.46 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=155.4, 80.2, 57.4 (minor), 55.5 (major), 35.5, 33.8, 29.4, 28.6, 28.2. HRMS (EI.sup.+): m/z calc. for C.sub.10H.sub.19NO.sub.2SSe [M].sup.+ 297.0296, found 297.0303.
N-Boc-N-methyl-1,2-diselenane-4-amine (X14)
[0444] ##STR00083##
[0445] X11 was methylated according to general procedure C: Starting material (200 mg, 0.608 mmol, 1.0 equiv.) in DMF (0.02 M, 40 mL) was sequentially charged with Mel (45 ?L, 0.729 mmol, 1.2 equiv.) and NaH (27 mg, 0.668 mmol, 1.1 equiv.) at 0? C. Following basic, aqueous workup, flash chromatography (hexanes/EtOAc 20:1) enabled isolation of pure X14 as a yellow solid (80 mg, 0.232 mmol, 38%). Note: the title compound was observed as a mixture of rotameric species. Reported NMR data refer to the major rotamer.
[0446] TLC R.sub.f=0.29 (hexanes/EtOAc 9:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=4.37 (s, 1H), 3.52-3.42 (m, 1H), 3.30 (d, J=13.0 Hz, 2H), 2.84-2.68 (m, 4H), 2.21 (d, J=13.3 Hz, 1H), 2.16-2.05 (m, 1H), 1.46 (s, 9H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=155.2, 80.1, 55.7, 34.6, 29.1, 28.6, 27.2, 25.6. HRMS (ESI?): m/z calc. for C.sub.10H.sub.18NO.sub.2Se.sub.2 [M?H].sup.? 343.9674, found 343.9677.
3-Bromo-1-morpholinopropan-1-one (X15)
[0447] ##STR00084##
[0448] To a solution of morpholine (0.81 mL, 9.4 mmol, 1.0 eq, 0.1 M in DCM) and triethylamine (2.0 mL, 14 mmol, 1.5 eq) was carefully added a solution of 3-bromo-propionyl chloride (1.0 mL, 9.4 mmol, 1.0 eq, 1.0 M in DCM) at 0? C. The mixture was stirred for 15 min, was allowed to warm to r.t. and was further stirred for 15 min. The mixture was quenched by addition of sat. aq. NaCl, the aqueous layer was extracted with EtOAc (three times) and the combined organic layers were washed with aq. NaCl were dried with Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to yield X15 as a colourless oil (1.51 g, 6.8 mmol, 72%). TLC R.sub.f=0.48 (EtOAc). .sup.1H-NMR (400 MHz, DMSO-d.sub.6): ? (ppm)=3.64 (t, J=6.7 Hz, 2H), 3.54 (dd, J=10.1, 5.2 Hz, 4H), 3.43 (q, J=5.0 Hz, 4H), 2.95 (t, J=6.7 Hz, 2H). .sup.13C-NMR (101 MHz, DMSO-d.sub.6): ? (ppm)=168.2, 66.1, 66.1, 45.2, 41.6, 35.2, 28.9. HRMS (ESI+): m/z calc. for C.sub.7H.sub.13N.sub.2OBr: [M+H].sup.+ 222.01242, found 222.01256.
3-Bromo-1-(4-methylpiperazin-1-yl)propan-1-one hydrochloride (X16)
[0449] ##STR00085##
[0450] 3-Bromo-propionyl chloride (3.0 mL, 28 mmol, 1.0 equiv.) was dissolved in dry DCM (30 mL, 1 M), and cooled to 0? C. N-Methyl-piperazine (3.1 mL, 28 mmol, 1.0 equiv.) in DCM (90 mL, 0.3 M), was added and the reaction mixture was stirred at retained temperature for 1 h. The colourless precipitates formed during the reaction were filtered, washed with ether and dried on air to give the title compound as a colourless solid (7.2 g, 26 mmol, 93%) TLC R.sub.f=0.40 (DCM/MeOH 9:1). .sup.1H-NMR (400 MHz, D.sub.2O): ? (ppm)=4.63 (br s, 1H), 4.23 (br s, 1H), 3.67 (t, J=6.2 Hz, 2H), 3.59 (br s, 3H), 3.13 (br s, 5H), 2.95 (q, J=1.8 Hz, 3H). HRMS (ESI+): m/z calc. for C.sub.8H.sub.16N.sub.2OBr: [M+H].sup.+ 235.04405, found 235.04418.
(S)-3-((1,2-Thiaselenan-4-yl)amino)-1-morpholinopropan-1-one hydrochloride (X17)
[0451] ##STR00086##
[0452] (S)-1,2-Thiaselenan-4-amine hydrochloride (0.10 g, 0.52 mmol, 1.2 equiv.), DIPEA (0.25 mL, 0.52 mmol, 1.2 equiv.) and X15 (86 mg, 0.40 mmol, 1.0 equiv.) were reacted in MeOH (1.2 mL, 0.3 M) according to general procedure D. Boc-protection using Boc.sub.2O (0.13 g, 0.60 mmol, 1.5 equiv.) and DIPEA (0.58 mL, 1.2 mmol, 3 equiv.) in DCM (2 mL, 0.2 M) gave X.sup.17a (49 mg, 0.12 mmol, 30%) after purification by flash chromatography (DCM.fwdarw.DCM/MeOH 9:1).
[0453] TLC R.sub.f=0.56 (EtOAc). HRMS (ESI+): m/z calc. for C.sub.16H.sub.28NaN.sub.2O.sub.4SSe: [M+Na].sup.+ 447.08272, found 447.08310.
[0454] Following general procedure E, hydrochloric acid (0.30 mL, 4 M in dioxane, 1.2 mmol, 10 equiv.) enabled Boc-deprotection of X6a in DCM (1.2 mL, 0.1 M). HCl-salt X17 (42 mg, 0.12 mmol, 30% over 3 steps) was obtained as a solid after filtration from the reaction mixture. .sup.1H-NMR (400 MHz, MeOD-d.sub.4): ? (ppm)=3.68 (dt, J=10.6, 4.7 Hz, 6H), 3.62-3.57 (m, 2H), 3.54-3.46 (m, 3H), 3.44-3.34 (m, 3H), 3.26-3.14 (m, 2H), 2.85 (t, J=5.9 Hz, 2H), 2.48 (d, J=11.3 Hz, 1H), 1.94 (d, J=10.9 Hz, 1H). HRMS (ESI+): m/z calc. for C.sub.11H.sub.21N.sub.2O.sub.4SSe: [M+H].sup.+ 325.04835, found 325.04865.
3-((1,2-Thiaselenan-5-yl)amino)-1-morpholinopropan-1-one hydrochloride (X18)
[0455] ##STR00087##
[0456] (S)-1,2-Thiaselenan-5-amine hydrochloride (0.11 g, 0.52 mmol, 1.3 equiv.), DIPEA (0.25 mL, 0.52 mmol, 1.3 equiv.) and X15 (89 mg, 0.40 mmol, 1.0 equiv.) were dissolved in MeOH (1.2 mL, 0.3 M) and reacted under microwave irradiation (120? C., 60 min) according to general procedure D. Subsequent protection using Boc.sub.2O (0.13 g, 0.60 mmol, 1.5 equiv.) and DIPEA (0.58 mL, 1.2 mmol, 3 equiv.) in DCM (2 mL, 0.2 M) followed by purification by flash chromatography (DCM.fwdarw.DCM/MeOH 9:1) gave X18a (49 mg, 0.12 mmol, 29%).
[0457] TLC R.sub.f=0.59 (EtOAc). HRMS (ESI+): m/z calc. for C.sub.16H.sub.28NaN.sub.2O.sub.4SSe: [M+Na].sup.+ 447.08272, found 447.08318.
[0458] Boc-deprotection using HCl (0.30 mL, 4 M in dioxane, 1.2 mmol, 10 equiv.) in DCM (1.2 mL, 0.1 M) according to general procedure E gave X18 (41 mg, 0.12 mmol, 29% over 3 steps) as a colourless solid. .sup.1H-NMR (400 MHz, MeOD-d.sub.4): ? (ppm)=3.71-3.64 (m, 6H), 3.62-3.58 (m, 2H), 3.55 (d, J=9.7 Hz, 1H), 3.54-3.49 (m, 2H), 3.42-3.33 (m, 3H), 3.31-3.25 (m, 1H), 3.14 (dd, J=13.8, 9.4 Hz, 1H), 2.86 (t, J=6.0 Hz, 2H), 2.61 (d, J=14.3 Hz, 1H), 2.25 (s, 1H). .sup.13C-NMR (101 MHz, MeOD-d.sub.4): ? (ppm)=170.4, 68.1, 67.6, 67.5, 46.9, 43.3, 42.2, 41.8, 35.1, 34.6, 29.8. HRMS (ESI+): m/z calc. for C.sub.11H.sub.21N.sub.2O.sub.4SSe: [M+H].sup.+ 325.04835, found 325.04859.
(S)-3-((1,2-Thiaselenan-4-yl)amino)-1-(4-methylpiperazin-1-yl)propan-1-one dihydrochloride (X19)
[0459] ##STR00088##
[0460] Prepared from (S)-1,2-thiaselenan-4-amine hydrochloride (0.31 g, 1.4 mmol, 1.4 equiv.), DIPEA (0.24 mL, 1.3 mmol, 1.3 equiv.) and X16 (0.28 g, 1.0 mmol, 1.0 equiv.) in MeOH (3 mL, 0.3 M) according to general procedure D. Boc-protection was implemented using Boc.sub.2O (0.34 g, 1.5 mmol, 1.5 equiv.) and DIPEA (0.55 mL, 3.1 mmol, 3 equiv.) in DCM (10 mL, 0.2 M). Purification by flash chromatography (DCM.fwdarw.DCM/MeOH 9:1) gave X19a as a colourless solid (0.15 g, 0.35 mmol, 35%).
[0461] TLC R.sub.f=0.54 (DCM/MeOH 9:1). HRMS (ESI+): m/z calc. for C.sub.17H.sub.32N.sub.3O.sub.3SSe: [M+H].sup.+ 438.13241, found 438.13276.
[0462] Using hydrochloric acid (0.88 mL, 4 M in dioxane, 3.5 mmol, 10 equiv.) in DCM (3.5 mL, 0.1 M), Boc-deprotection of X19a according to general procedure E, gave X19 (0.13 g, 0.32 mmol, 91%) as a pale yellow solid after filtration of the reaction mixture.
[0463] .sup.1H-NMR (400 MHz, MeOD-d.sub.4): ? (ppm)=4.69 (d, J=10.9 Hz, 1H), 4.15 (d, J=15.8 Hz, 1H), 3.71-3.62 (m, 2H), 3.55 (dd, J=18.0, 7.9 Hz, 3H), 3.39 (dd, J=11.5, 5.8 Hz, 2H), 3.28-3.16 (m, 2H), 3.10 (d, J=9.1 Hz, 2H), 3.06-2.98 (m, 1H), 2.95 (s, 3H), 2.91-2.80 (m, 1H), 2.51 (d, J=8.9 Hz, 1H), 2.03-1.84 (m, 1H). .sup.13C-NMR (101 MHz, MeOD-d.sub.4) ? (ppm)=170.4, 54.5, 51.2, 43.6, 43.4, 41.9, 39.4, 33.4, 30.0, 25.4. HRMS (ESI+): m/z calc. for C.sub.11H.sub.21N.sub.2O.sub.4SSe: [M+H].sup.+ 338.07998, found 338.08022.
(S)-3-((1,2-Thiaselenan-5-yl)amino)-1-(4-methylpiperazin-1-yl)propan-1-one dihydrochloride (X20)
[0464] ##STR00089##
[0465] Following general procedure D, (S)-1,2-thiaselenan-5-amine hydrochloride (0.44 g, 2.0 mmol, 1.35 equiv.), DIPEA (0.35 mL, 1.9 mmol, 1.3 equiv.) and X16 (0.40 g, 1.5 mmol, 1.0 equiv.), were dissolved in MeOH (5 mL, 0.3 M) and reacted under microwave irradiation (120? C., 60 min). Boc-protection of the crude diamine was conducted using Boc.sub.2O (0.48 g, 2.2 mmol, 1.5 equiv.) and DIPEA (0.79 mL, 4.4 mmol, 3 equiv.) in DCM (7.5 mL, 0.2 M). X20a was received as a colourless solid (0.17 g, 0.38 mmol, 25%) after purification via flash column chromatography (DCM.fwdarw.DCM/MeOH 9:1).
[0466] TLC R.sub.f=0.40 (DCM/MeOH 9:1). HRMS (ESI+): m/z calc. for C.sub.17H.sub.32N.sub.3O.sub.3SSe: [M+H].sup.+ 438.13241, found 438.13263.
[0467] Deprotection of X20a was achieved according to general procedure E. Starting material (0.17 g, 0.38 mmol, 1.0 equiv.) in DCM (4 mL, 0.1 M) was charged with HCl (0.95 mL, 4 M in dioxane, 3.8 mmol, 10 equiv.) and the title compound was collected through filtration of the reaction mixture (0.15 g, 0.36 mmol, 95%).
[0468] .sup.1H-NMR (400 MHz, MeOD-d.sub.4): ? (ppm)=4.68 (d, J=11.2 Hz, 1H), 4.15 (d, J=15.4 Hz, 1H), 3.71-3.65 (m, 1H), 3.63-3.49 (m, 5H), 3.38 (t, J=5.7 Hz, 3H), 3.27 (d, J=11.0 Hz, 2H), 3.18-3.04 (m, 4H), 2.95 (s, 3H), 2.61 (d, J=9.1 Hz, 1H), 2.24 (d, J=8.1 Hz, 1H). .sup.13C-NMR (101 MHz, MeOD-d.sub.4) ? (ppm)=170.5, 54.1, 51.2, 43.6, 43.4, 41.6, 40.4, 34.3, 32.1, 29.6. HRMS (ESI+): m/z calc. for C.sub.11H.sub.21N.sub.2O.sub.4SSe: [M+H].sup.+ 338.04835, found 338.08024.
6-Chloro-2-(5-chloro-2-hydroxyphenyl)quinazolin-4(3H)-one (X21)
[0469] ##STR00090##
[0470] To a solution of 5-chloro-salicylaldehyde (5.00 g, 31.9 mmol, 1.0 equiv.) in anhydrous ethanol (0.05 M, 620 mL), 2-amino-5-chlorobenzamide (5.45 g, 31.9 mmol, 1.0 equiv.) and p-toluene sulfonic acid (607 mg, 3.19 mmol, 0.1 equiv.) were added and the resulting suspension was heated to 90? C. for 1 h. The reaction mixture was cooled to 0? C. and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ, 7.25 g, 31.9 mmol, 1.0 equiv.) was added in small batches. The reaction mixture was further stirred at retained temperature for 1 h upon which a yellow precipitate occurred. The solid was filtered, washed with ice-cold ethanol and dried to afford the title compound as a beige solid (8.40 g, 27.3 mmol, 86%). When excited with UV-light, X21 exhibited bright green fluorescence. NMR spectra match reported data (Thorn-Seshold; Chem. Commun. 2012 48, 6253-6255).
[0471] .sup.1H NMR (400 MHz, DMSO) ? (ppm)=13.34 (s, 1H), 12.74 (s, 1H), 8.29 (d, J=2.6 Hz, 1H), 8.09 (d, J=2.4 Hz, 1H), 7.90 (dd, J=8.7, 2.5 Hz, 1H), 7.84 (d, J=8.7 Hz, 1H), 7.49 (dd, J=8.9, 2.6 Hz, 1H), 7.04 (d, J=8.8 Hz, 1H). HRMS (ESI?): m/z calc. for C.sub.14H.sub.7Cl.sub.2N.sub.2O.sub.2 [M?H].sup.? 304.9890, found 304.9891.
4-Chloro-2-(6-chloro-4-oxo-3,4-dihydroquinazolin-2-yl)phenyl chloroformate (X22)
[0472] ##STR00091##
[0473] Under a nitrogen atmosphere, 6-chloro-2-(5-chloro-2-hydroxyphenyl) quinazolin-4-one (X21, 1.44 g, 4.70 mmol, 1.0 equiv.) was suspended in dry dichloromethane (0.25 M, 20 mL). NEt.sub.3 (1.30 mL, 9.40 mmol, 2.0 equiv.) was added and the mixture was cooled to 0? C. The mixture was then slowly charged with triphosgene (1.46 g, 4.94 mmol, 1.05 equiv.) in dry DCM (0.3 M, 18 mL) and an immediate formation of HCl could be observed. The reaction mixture was stirred at retained temperature for 30 min, was then allowed to warm to r.t. and was further stirred for 30 min. Exposure of the reaction mixture to 366 nm UV light allowed assessment of the reaction progress: starting material X21 shows bright green fluorescence under these conditions, while the target species exhibits weak, blue fluorescence. Upon full conversion, which was indicated by LCMS, all volatile compounds were removed using two serially connected, external liquid nitrogen traps while carefully exposing the reaction vessel to reduced pressure. Excess phosgene which was caught in either of the liquid nitrogen traps was destroyed when still cold by carefully adding an excess of piperidine. Chloroformate X22 was received as a beige solid and was used without further purification. For following carbamate coupling reactions, the crude solid was resuspended in dry DCM (0.04 M, 118 mL).
Compounds of the Invention: Fluorogenic Probes
Me-SeS60-PQ (P1)
[0474] ##STR00092##
[0475] Boc-deprotection of X12 was achieved following general procedure E. A solution of the starting material (70 mg, 0.236 mmol, 1.0 equiv.) in DCM (0.1 M, 2.5 mL) was charged with HCl (4 M in dioxane, 0.591 mL, 2.36 mmol, 10 equiv.) and the deprotected amine was obtained as its HCl salt and as a pale yellow solid. After re-dissolution in DCM (0.05 M, 5 mL) and NEt.sub.3 (0.10 mL, 0.72 mmol, 3.0 equiv.), the amine was added to chloroformate X22 (0.25 M in DCM, 2.0 mL, 0.50 mmol, 2.0 equiv.) according to general procedure F. Within 2 h, full conversion was achieved and target compound P1 was isolated (92 mg, 0.174 mmol, 74%) after aqueous workup and column chromatography (hexanes:EtOAc 3:1). Note: the title compound was observed as a mixture of rotameric species. Reported NMR data refer to the major rotamer.
[0476] TLC R.sub.f=0.15 (hexanes/EtOAc 3:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=10.23 (s, 1H), 8.25 (d, J=2.2 Hz, 1H), 7.98 (dd, J=10.8, 2.6 Hz, 1H), 7.76-7.70 (m, 2H), 7.51 (dt, J=8.8, 2.1 Hz, 1H), 7.18 (dd, J=8.8, 1.6 Hz, 1H), 4.39 (tt, J=11.9, 3.1 Hz, 1H), 3.35-3.20 (m, 3H), 3.03 (s, 3H), 2.69 (dd, 1H), 2.07 (dd, J=13.3, 3.7 Hz, 1H), 2.02-1.90 (m, 1H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=160.8, 153.9, 149.4, 147.6, 147.5, 135.5, 133.5, 132.5, 132.3, 130.6, 129.7, 128.1, 126.2, 125.3, 122.4, 56.7, 35.9, 33.5, 30.0, 26.1. HRMS (ESI+): m/z calc. for C.sub.20H.sub.18Cl.sub.2N.sub.3O.sub.3SSe [M+H].sup.+ 529.9606, found 529.9612.
Me-SSe60-PQ (P2)
[0477] ##STR00093##
[0478] Combining general procedures E and F, thiaselenane X13 (100 mg, 0.338 mmol, 1.0 equiv.) in DCM (0.1 M, 4 mL) was charged with HCl (4 M in dioxane, 0.84 mL, 3.4 mmol, 10 equiv.). Upon full conversion, the solvent was removed, and the residue was re-dissolved in DCM (0.05 M, 8 mL) and NEt.sub.3 (0.140 mL, 1.01 mmol, 3.0 equiv.). A solution of chloroformate X22 in DCM (0.25 M, 2.60 mL, 0.660 mmol, 2.0 equiv.) was then charged with the deprotected amine and, after workup and column chromatography (hexanes:EtOAc 3:1), P2 was received as a pale yellow solid (83 mg, 0.157 mmol, 46%). Note: the title compound was observed as a mixture of rotameric species. Reported NMR data refer to the major rotamer.
[0479] TLC R.sub.f=0.14 (hexanes/EtOAc 3:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=10.15 (s, 1H), 8.30-8.22 (m, 1H), 7.98 (t, J=3.3 Hz, 1H), 7.79-7.67 (m, 2H), 7.51 (dd, J=8.7, 2.5 Hz, 1H), 7.17 (dd, J=8.8, 3.7 Hz, 1H), 4.23-4.15 (m, 1H), 3.47-3.35 (m, 1H), 3.19 (q, J=11.5 Hz, 1H), 3.12-2.94 (m, 4H), 2.79 (dd, J=13.0, 3.0 Hz, 1H), 2.36-2.14 (m, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=160.7, 153.9, 149.3, 147.6, 147.5, 135.5, 133.6, 132.5, 132.4, 130.6, 129.7, 128.0, 126.2, 125.3, 122.4, 57.8, 35.0, 33.1, 30.5, 27.7. HRMS (ESI+): m/z calc. for C.sub.20H.sub.18Cl.sub.2N.sub.3O.sub.3SSe [M+H].sup.+ 529.9606, found 529.9603.
Me-SeSe60-PQ (P3)
[0480] ##STR00094##
[0481] HPQ-coupling of X14 was conducted according to general protocols E and F. Following deprotection of the starting material (70 mg, 0.204 mmol, 1.0 equiv.) by HCl (4 M in dioxane, 0.510 mL, 2.04 mmol, 10 equiv.), a solution of the amine-HCl salt in DCM (0.05 M, 6 mL) and NEt.sub.3 (85 ?L, 0.612 mmol, 3.0 equiv.) was reacted with chloroformate X22 (0.25 M in DCM, 1.70 mL, 0.440 mmol, 2.0 equiv.) for 4 h. Upon completion, aqueous workup and flash chromatography (hexanes:EtOAc 3:1) gave P3 as a yellow solid (67 mg, 0.116 mmol, 57%). Note: the title compound was observed as a mixture of rotameric species. Reported NMR data refer to the major rotamer.
[0482] TLC R.sub.f=0.18 (hexanes/EtOAc 3:1) .sup.1H NMR (400 MHz, CDCl.sub.3) ? (ppm)=10.11 (s, 1H), 8.26 (dd, J=2.1, 0.9 Hz, 1H), 7.99 (dd, J=6.0, 2.6 Hz, 1H), 7.77-7.70 (m, 2H), 7.54-7.49 (m, 1H), 7.18 (d, J=8.7 Hz, 1H), 4.33 (t, J=11.8 Hz, 1H), 3.46 (t, J=12.9 Hz, 1H), 3.42-3.24 (m, 2H), 3.02 (s, 3H), 2.78 (d, J=11.9 Hz, 1H), 2.25-2.09 (m, 2H). .sup.13C NMR (101 MHz, CDCl.sub.3) ? (ppm)=160.7, 153.8, 149.3, 147.6, 147.5, 135.5, 133.6, 132.5, 132.4, 130.7, 129.8, 128.1, 126.2, 125.3, 122.4, 58.1, 34.2, 30.1 HRMS (ESI+): m/z calc. for C.sub.20H.sub.18Cl.sub.2N.sub.3O.sub.3Se.sub.2 [M+H].sup.+ 577.9050, found 577.9048.
M-SeS60-PQ (P4)
[0483] ##STR00095##
[0484] Prepared from X17 (40 mg, 0.11 mmol, 1.0 equiv.) and X22 (3.0 mL, 0.04 M in DCM, 0.12 mmol, 1.1 equiv.) according to general protocol F. Purification by flash chromatography (isohexane/EtOAc) gave P4 as a colourless solid (43 mg, 0.065 mmol, 59%). Light blue solid-state fluorescence was observed under UV-light.
[0485] TLC R.sub.f=0.48 (EtOAc). .sup.1H-NMR (400 MHz, CDCl.sub.3): ? (ppm)=10.46 (d, J=64.1 Hz, 1H), 8.22 (d, J=3.0 Hz, 1H), 7.92 (dd, J=16.2, 2.3 Hz, 1H), 7.78-7.68 (m, 2H), 7.56-7.47 (m, 1H), 7.17 (dd, J=8.6, 2.7 Hz, 1H), 4.49-4.12 (m, 1H), 3.73 (s, 1H), 3.68 (dd, J=8.5, 3.7 Hz, 3H), 3.66-3.58 (m, 5H), 3.52 (d, J=12.7 Hz, 3H), 3.50-3.46 (m, 1H), 3.43-3.35 (m, 1H), 3.33 (d, J=13.5 Hz, 1H), 3.30-3.17 (m, 2H), 2.82-2.67 (m, 1H), 2.64-2.58 (m, 1H), 2.58-2.47 (m, 1H), 2.22-2.01 (m, 2H). .sup.13C-NMR (101 MHz, CDCl.sub.3) ? (ppm)=168.9, 168.5, 160.8, 153.7, 149.6, 147.5, 147.1, 135.5, 133.6, 132.5, 130.7, 129.8, 128.6, 126.0, 125.1, 124.9, 122.3, 67.2, 67.0, 66.7, 66.5, 58.6, 46.0, 45.8, 42.2, 42.0, 40.0, 36.2, 36.0, 34.8, 33.6, 32.6, 27.4. HRMS (ESI+): m/z calc. for C.sub.26H.sub.27Cl.sub.2N.sub.5O.sub.4SSe: [M+H].sup.+ 657.02389, found 657.02417.
M-SSe60-PQ (P5)
[0486] ##STR00096##
[0487] Prepared from X18 (40 mg, 0.11 mmol, 1.0 equiv.) and X22 (3.0 mL, 0.04 M in DCM, 0.12 mmol, 1.1 equiv.) according to general protocol F. Purification by flash chromatography (isohexane/EtOAc) gave P5 as a colourless solid (58 mg, 0.088 mmol, 80%). Light blue solid-state fluorescence was observed under UV-light.
[0488] TLC R.sub.f=0.46 (EtOAc). .sup.1H-NMR (400 MHz, CDCl.sub.3): ? (ppm)=10.48 (d, J=52.1 Hz, OH), 8.25-8.18 (m, 1H), 7.91 (dd, J=24.5, 2.5 Hz, 1H), 7.80-7.68 (m, 2H), 7.51 (td, J=7.9, 7.0, 2.5 Hz, 1H), 7.21-7.11 (m, 1H), 4.33-3.88 (m, 1H), 3.77-3.71 (m, 1H), 3.71-3.66 (m, 4H), 3.66-3.58 (m, 5H), 3.53 (d, J=14.5 Hz, 4H), 3.50-3.45 (m, 2H), 3.43-3.20 (m, 4H), 3.07-2.98 (m, 1H), 2.88 (d, J=10.6 Hz, 1H), 2.78 (d, J=7.0 Hz, 1H), 2.59 (t, J=7.3 Hz, 1H), 2.51 (q, J=7.2 Hz, 1H), 2.40-2.27 (m, 2H), 2.21 (s, 1H). HRMS (ESI+): m/z calc. for C.sub.26H.sub.27Cl.sub.2N.sub.5O.sub.4SSe: [M+H].sup.+ 657.02389, found 657.02401.
P-SeS60-PQ (P6)
[0489] ##STR00097##
[0490] Prepared from X19 (20 mg, 0.049 mmol, 1.0 equiv.) and X22 (1.4 mL, 0.04 M in DCM, 0.054 mmol, 1.1 equiv.) according to general protocol F. Purification by flash chromatography (DCM.fwdarw.DCM/MeOH 9:1) gave P6 as a colourless solid (20 mg, 0.030 mmol, 61%). Further purification by preparative HPLC (MeCN/H.sub.2O, 0.1% FA) yielded P6 as a colourless solid. Light blue solid-state fluorescence was observed under UV-light.
[0491] TLC R.sub.f=0.45 (DCM/MeOH 9:1). .sup.1H-NMR (400 MHz, MeOD-d.sub.4): ? (ppm)=.sup.1H NMR (500 MHz, MeOD-d.sub.4) ? 8.22 (d, J=2.0 Hz, 1H), 7.91-7.85 (m, 1H), 7.83 (d, J=2.4 Hz, 1H), 7.76 (dd, J=8.6, 6.8 Hz, 1H), 7.65 (dt, J=8.5, 3.3 Hz, 1H), 7.42-7.34 (m, 1H), 4.48 (s, OH), 3.89 (d, J=12.1 Hz, 1H), 3.72-3.64 (m, 1H), 3.59 (s, 1H), 3.52 (s, 2H), 3.40 (d, J=3.6 Hz, 1H), 3.36 (d, J=11.1 Hz, 1H), 3.30-3.22 (m, 2H), 3.21-3.16 (m, 1H), 3.16-3.08 (m, 1H), 2.89 (d, J=9.5 Hz, 1H), 2.83-2.74 (m, 1H), 2.56 (d, J=11.2 Hz, 1H), 2.48 (d, J=14.1 Hz, 2H), 2.41 (d, J=4.5 Hz, 2H), 2.36 (d, J=7.0 Hz, 3H), 2.32 (s, 1H), 2.20 (d, J=13.2 Hz, 1H), 2.10-1.96 (m, 2H), 1.88 (d, J=12.5 Hz, 1H). .sup.13C-NMR (101 MHz, MeOD-d.sub.4) ? (ppm)=169.9, 169.6, 162.0, 152.5, 147.5, 135.1, 132.7, 131.6, 131.0, 129.6, 128.9, 125.1, 124.7, 124.6, 122.1, 58.0, 56.1, 54.4, 54.3, 53.8, 44.4, 43.0, 40.7, 40.6, 35.8, 34.4, 33.2, 33.0, 32.0, 26.9, 25.9, 15.9, 15.7. HRMS (ESI+): m/z calc. for C.sub.27H.sub.30Cl.sub.2N.sub.5O.sub.4SSe: [M+H].sup.+ 670.05553, found 670.05587.
P-SSe60-PQ (P7)
[0492] ##STR00098##
[0493] Prepared from X20 (12 mg, 0.030 mmol, 1.0 equiv.) and X22 (0.83 mL, 0.04 M in DCM, 0.033 mmol, 1.1 equiv.) according to general protocol F. Purification by flash chromatography (DCM-DCM/MeOH 9:1) gave P7 as a colourless solid (13 mg, 0.020 mmol, 66%). Further purification by preparative HPLC (MeCN/H.sub.2O, 0.1% FA) yielded P7 as a colourless solid. Light blue solid-state fluorescence was observed under UV-light.
[0494] TLC R.sub.f=0.44 (DCM/MeOH 9:1). .sup.1H-NMR (400 MHz, MeOD-d.sub.4): ? (ppm)=? 8.22 (d, J=2.4 Hz, 1H), 7.88 (dt, J=8.7, 3.1 Hz, 1H), 7.83 (d, J=2.6 Hz, 1H), 7.79-7.74 (m, 1H), 7.65 (ddd, J=7.6, 4.8, 2.6 Hz, 2H), 7.39 (t, J=8.4 Hz, 1H), 4.27 (s, 1H), 3.73-3.63 (m, 2H), 3.58 (s, 1H), 3.54-3.48 (m, 2H), 3.48-3.42 (m, 1H), 3.39 (dd, J=11.1, 4.9 Hz, 1H), 3.30-3.20 (m, 3H), 3.10 (d, J=13.2 Hz, 1H), 3.02-2.91 (m, 1H), 2.79-2.73 (m, 1H), 2.58 (d, J=10.9 Hz, 1H), 2.48-2.40 (m, 2H), 2.39-2.35 (m, 2H), 2.33 (d, J=8.3 Hz, 3H), 2.27 (d, J=22.8 Hz, 2H), 1.98 (d, J=12.6 Hz, 1H). .sup.13C-NMR (101 MHz, MeOD-d.sub.4) ? (ppm)=169.9, 169.6, 152.6, 147.5, 135.0, 135.0, 132.7, 131.6, 131.0, 129.6, 129.0, 125.1, 124.7, 124.5, 122.1, 60.2, 56.2, 56.1, 54.5, 54.4, 54.0, 53.9, 44.5, 40.8, 40.7, 35.5, 34.5, 34.1, 32.9, 32.0, 27.6, 16.0, 15.9, 15.7. HRMS (ESI+): m/z calc. for C.sub.27H.sub.30C.sub.12N.sub.5O.sub.4SSe: [M+H].sup.+ 670.05553, found 670.05587.
Reference Fluorogenic Probes
Di-tert-butyl (disulfanediylbis(ethane-2,1-diyl))dicarbamate (X24)
[0495] ##STR00099##
[0496] Cysteamine hydrochloride X23 (1.00 g, 8.8 mmol) was dissolved in MeOH (30 mL) and the free base cysteamine was generated by addition of KOH (1.16 g, 17.6 mmol). Addition of methanolic I.sub.2 until a slight colour persisted permanently, followed by evaporation of all volatiles gave 2-(2-aminoethyldisulfanyl)ethanamine that was N-Boc protected according using Boc.sub.2O (2.2 equiv.) and DIPEA (2.5 equiv.) in dioxane/water (1:1, 0.5 M) gave X24 (0.70 g, 2.0 mmol, 45%) as colourless crystalline needles after recrystallization from MeOH.
2,2-Disulfanediylbis(N-methylethan-1-amine) dihydrochloride (X25)
[0497] ##STR00100##
[0498] Step 1: X24 (0.78 g, 2.21 mmol) was dissolved in anhydrous DMF (0.02 M) and solid NaH (2.2 eq) was added at 0? C., followed by addition of Mel (2.3 eq) in one portion. The mixture was stirred for 0.5 h, then allowed to warm to r.t. and stirred for a further hour. The mixture was diluted with EtOAc and washed with sat. aq. NaCl (2?). The combined aq. layers were extracted with EtOAc (3?50 mL) and the combined organic layers were dried over Na.sub.2SO.sub.4. filtered and concentrated and purified by FCC to afford di-tert-butyl (disulfanediylbis(ethane-2,1-diyl))bis(methylcarbamate) as a colourless solid.
[0499] Step 2: Deprotection of the material obtained in step 1 was achieved according to general procedure E and the resulting diamine dihydrochloride X25 (0.46 mg, 1.82 mmol, 82%) was obtained as a colourless solid.
[0500] .sup.1H-NMR (400 MHz, DMSO-d.sub.6): ? (ppm)=9.27 (s, 4H), 3.19 (d, J=7.4 Hz, 4H), 3.09 (dd, J=8.1, 5.7 Hz, 4H), 2.56 (s, 6H). .sup.13C-NMR (101 MHz, DMSO-d.sub.6): ? (ppm)=47.0, 32.4, 32.3.
4-chloro-2-(6-chloro-4-oxo-3,4-dihydroquinazolin-2-yl)phenyl methyl(2-((2-(methyl amino)ethyl)disulfaneyl)ethyl)carbamate (X26)
[0501] ##STR00101##
[0502] X22 was coupled to X25 (715 mg, 2.82 mmol) according to general protocol F. Purification by FCC (DCM/MeOH) gave X26 as a colourless powder (245 mg, 0.477 mmol, 44%).
[0503] R.sub.f=0.22 (DCM/MeOH 9:1). .sup.1H-NMR (400 MHz, tetrachlorethane-d.sub.2, 373 K): ? (ppm)=8.20 (t, J=1.5 Hz, 1H), 7.87 (d, J=2.6 Hz, 1H), 7.71 (d, J=1.5 Hz, 2H), 7.48 (dd, J=8.7, 2.6 Hz, 1H), 7.24 (d, J=8.7 Hz, 1H), 3.62 (s, 2H), 3.23 (d, J=6.2 Hz, 2H), 3.18 (d, J=6.1 Hz, 2H), 3.03 (s, 3H), 2.88 (s, 2H), 2.66 (s, 3H). .sup.13C-NMR (101 MHz, DMSO-d.sub.6): ? (ppm)=160.8, 153.1, 150.6, 147.9, 147.2, 134.9, 131.5, 131.4, 130.1, 129.6, 129.4, 128.5, 125.2, 125.0, 122.4, 47.7, 47.0, 35.1, 34.6, 32.4, 32.3. HRMS (ESI+) m/z calc. for C.sub.21H.sub.23C.sub.12N.sub.4O.sub.3S.sub.2[M+H].sup.+ 513.05831, found 513.05886.
3-(Cyclohexylamino)-1-(4-methylpiperazin-1-yl)propan-1-one dihydrochloride (X27)
[0504] ##STR00102##
[0505] According to general procedure D, cyclohexylamine (57 ?L, 0.50 mmol, 1.25 eq) and X16 (77 mg, 0.40 mmol, 1.0 equiv.) were reacted in MeOH (1.2 mL, 0.3 M) under microwave irradiation (120? C., 60 min). Boc-protection using Boc.sub.2O (0.13 g, 0.60 mmol, 1.5 equiv.) and DIPEA (0.58 mL, 1.2 mmol, 3 equiv.) in DCM (2 mL, 0.2 M) gave X27a (0.12 g, 0.32 mmol, 81%) upon isolation by flash chromatography (DCM.fwdarw.DCM/MeOH 9:1).
[0506] TLC R.sub.f=0.45 (DCM/MeOH 9:1). HRMS (ESI+): m/z calc. for C.sub.19H.sub.36N.sub.3O.sub.3: [M+H].sup.+ 354.27512, found 354.27544.
[0507] Boc-deprotection was conducted following general procedure E. Intermediate X27a (46 mg, 0.13 mmol, 1.0 equiv.) was dissolved in DCM (1.3 mL, 0.1 M) and reacted with HCl (0.33 mL, 4 M in dioxane, 1.3 mmol, 10 equiv.). Filtration of the precipitates formed during the reaction gave compound X27 (42 mg, 0.13 mmol, 80% over 3 steps) as a colourless solid which was used without further analysis or purification.
P-CC60-PQ (X28)
[0508] ##STR00103##
[0509] Prepared from X27 (42 mg, 0.13 mmol, 1.0 equiv.) and X22 (3.5 mL, 0.04 M in DCM, 0.14 mmol, 1.1 equiv.) according to general protocol F. Purification by flash chromatography (DCM.fwdarw.DCM/MeOH 9:1) gave X28 as a colourless solid (55.0 mg, 0.088 mmol, 68%). Further purification by preparative HPLC (MeCN/H.sub.2O, 0.1% FA) yielded X28 as a colourless solid. Light blue solid-state fluorescence was observed under UV-light.
[0510] TLC R.sub.f=0.55 (DCM/MeOH 9:1). .sup.1H-NMR (400 MHz, MeOD-d.sub.4): ? (ppm)=12.73 (s, 1H), 8.08 (d, J=4.4 Hz, 1H), 7.88 (t, J=7.7 Hz, 1H), 7.85-7.78 (m, 1H), 7.65 (dt, J=14.4, 8.3 Hz, 2H), 7.37 (dd, J=8.7, 3.7 Hz, 1H), 3.89 (s, 1H), 3.53-3.43 (m, 2H), 3.38 (s, 2H), 3.25 (s, 1H), 3.22-3.14 (m, 1H), 2.97 (s, 1H), 2.64-2.54 (m, 1H), 2.16 (d, J=3.5 Hz, 6H), 2.09-2.02 (m, 1H), 1.66 (s, 2H), 1.60 (d, J=12.8 Hz, 1H), 1.57-1.45 (m, 1H), 1.35 (t, J=10.5 Hz, 3H), 1.30-0.88 (m, 4H). .sup.13C-NMR (101 MHz, DMSO-d.sub.6) ? (ppm)=168.8, 168.3, 152.6, 152.2, 148.0, 147.7, 134.6, 131.1, 130.2, 130.0, 129.2, 125.4, 124.9, 122.4, 56.4, 56.1, 54.8, 54.7, 54.3, 54.2, 45.7, 44.6, 44.3, 40.8, 40.7, 33.2, 31.9, 30.6, 29.7, 25.4, 24.8. HRMS (ESI+): m/z calc. for C.sub.29H.sub.34C.sub.12N.sub.5O.sub.4: [M+H].sup.+ 586.19824, found 586.19933.
Precursors to Therapeutic Prodrugs
5-(3-(Azetidine-1-yl)propoxy-1H-indole-2-carboxylic acid (X29)
[0511] ##STR00104##
[0512] Step 1: 5-hydroxy-1H-indole-2-carboxylic acid (1.27 g, 7.15 mmol) was dissolved in MeOH (14 mL) and cooled to 0C. SOCl.sub.2 (0.78 mL, 1.28 g, 10.7 mmol) was added dropwise and the resulting mixture was heated to 75? C. for 2 h, then cooled to 25? C. and diluted with EtOAc (100 mL). The organic layer was washed with sat. aq. NaHCO.sub.3 and sat. aq. NaCl and the combined aq. layers were extracted with EtOAc (3?50 mL). The combined organic layers were dried over Na.sub.2SO.sub.4, filtered, concentrated under reduced pressure and purified by FCC (isohexane/EtOAc) to give methyl 5-hydroxy-1H-indole-2-carboxylate as a colourless powder (1.32 g, 6.9 mmol, 97%).
[0513] Step 2: PPh.sub.3 (125 mg, 0.52 mmol) was dissolved in THF (0.25 M) and cooled to 0? C. DIAD (94 ?L, 0.48 mmol) was added dropwise and the mixture was stirred at r.t. for 30 min. A solution of 3-(azetidin-1-yl)propan-1-ol (0.5 M) in THF and a solution of methyl 5-hydroxy-1H-indole-2-carboxylate (0.5 M) in THF were added subsequently. The mixture was allowed to warm to r.t. for 1 h, was then further stirred for 15 h, before being concentrated under reduced pressure and purified using FCC to give methyl 5-(3-(azetidin-1-yl)propoxy)-1H-indole-2-carboxylate (76 mg, 0.27 mmol, 62%) as a colourless oil.
[0514] Step 3: Methyl 5-(3-(azetidin-1-yl)propoxy)-1H-indole-2-carboxylate (222 mg, 0.77 mmol) was dissolved in MeOH:H.sub.2O (3:1, 40 mL) and solid KOH (61 mg, 0.92 mmol) was added. The resulting solution was heated to 75? C. for 15 h, was cooled to r.t. and a solution of HCl (0.74 mL, 1.25 M in MeOH) was added. The resulting heterogenous mixture was concentrated under reduced pressure to obtain compound X.sup.29 (303 mg, 99%. 68% w/w calc. purity) contaminated with KCl. An analytically pure sample was separately obtained from RP chromatography (H.sub.2O/MeCN) giving X.sup.29 as a colourless powder.
[0515] .sup.1H-NMR (400 MHz, DMSO-d.sub.6): ? (ppm)=11.14 (d, J=2.2 Hz, 1H), 7.25 (d, J=8.8 Hz, 1H), 7.08 (d, J=2.4 Hz, 1H), 6.76 (d, J=2.1 Hz, 2H), 6.74 (d, J=2.4 Hz, 1H), 4.02 (t, J=6.8 Hz, 2H), 3.63 (t, J=7.5 Hz, 4H), 2.91 (t, J=6.5 Hz, 2H), 2.20 (p, J=7.5 Hz, 2H), 1.86 (p, J=6.7 Hz, 2H). .sup.13C-NMR (101 MHz, DMSO-d.sub.6): ? (ppm)=165.1, 152.6, 134.1, 131.7, 127.6, 114.3, 112.9, 104.3, 102.6, 65.3, 53.2, 53.0, 25.4, 16.2. HRMS (ESI?) m/z calc. for C.sub.13H.sub.13NO.sub.2Cl [M?H].sup.?: calc. 275.13902, found 275.13910.
tert-Butyl (1S)-1-(chloromethyl)-5-((methyl((S)-1,2-thiaselenan-4-yl)carbamoyl)oxy)-2,3-dihydro-1H-3A.SUP.4.-benzo[e]indole-3-carboxylate (X30)
[0516] ##STR00105##
[0517] Step 1: Following Tietze et al. (Tietze, L. F. et al. ChemMedChem 2008, 3, 1946-1955. https://doi.org/10.1002/cmdc.200800250), to commercial (S)-3-(Boc)-5-(benzyloxy)-1-(chloromethyl)-1,2-dihydro-3H-benzo[e]indole (591 mg, 1.39 mmol) were added dry THF (0.05 M), Pd/C (297 mg, 10% on charcoal), and aq. NH.sub.4HCO.sub.2 (2.8 mL of a 4 M aq. solution). The mixture was stirred for 80 min, filtered over Kieselgur and washed with EtOAc (20 mL) and the filtrates were concentrated under reduced pressure to obtain (S)-3-(Boc)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indole as a colourless solid (460 mg, 1.38 mmol, 99%).
[0518] Step 2: An oven-dried Schlenk flask was charged with (S)-3-(Boc)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indole (88 mg, 0.30 mmol, 1.0 equiv.) under inert gas atmosphere, the compound was dissolved in anhydrous DCM (0.1-0.2 M) and the resulting solution was cooled to 0? C. Triphosgene (1.2 eq, 0.05 M solution in anhydrous DCM) was added in one portion and DIPEA (4.0 eq, 0.05 M solution in anhydrous DCM) was added. Amine mediated in-situ formation of phosgene and hydrochloride was indicated, the resulting mixture was further stirred at 0? C. for 0.5 h, was then allowed to warm to r.t. and was further stirred for 0.5 h. All volatile compounds were removed using an external liquid nitrogen trap to afford the corresponding chloroformate derivative as a solid. The trapped residue mixture containing residual phosgene was quenched by subsequently adding an aq. solution of NaOH (2 M) and diisopropylamine (10 eq).
[0519] Step 3: X12 (95 mg, 0.32 mmol, 1.0 equiv.) was deprotected according to general procedure E, using HCl (4 M in dioxane, 3.2 mmol, 10 equiv.) in DCM (0.1 M, 3 mL). The intermediary amine hydrochloride X30a was isolated through filtration of the reaction mixture and used without further purification.
[0520] Step 4: An oven-dried round-bottom flask was charged with (R)-N-methyl-1,2-thiaselenan-4-amine hydrochloride X30a (69 mg, 0.30 mmol, 1.0 equiv.) and the compound was suspended in anhydrous DCM (0.02 M). DIPEA (2.4 eq, 0.05 M solution in anhydrous DCM) was added to afford a clear solution of the corresponding free amine. A solution (0.01 M in anhydrous DCM) of the chloroformate synthesised in Step 2 was added dropwise to the reaction flask at 0? C. and the resulting mixture was further stirred for 0.5 h, was then allowed to warm to r.t. and was further stirred for 1-2 h. The reaction was stopped by adding a sat. aq. solution of NaHCO.sub.3 (50 mL), the mixture was diluted with DCM. The organic layer was seperated and washed with a sat. aq. solution of sodium chloride (2?50 mL). The combined aq. layers were extracted with DCM (2?100 mL) and the combined organic layers were dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to afford the crude product as a coloured solid.
[0521] Purification was achieved using FCC and the desired product X30 was obtained as a colourless solid (84 mg, 0.15 mmol, 51%).
[0522] NMR experiments at r.t. showed two rotameric speciesonly signals for the major rotamer are reported. .sup.1H NMR (500 MHz, CDCl.sub.3) ? (ppm)=8.05 (br-s, 1H), 7.78 (dd, J=20.5, 8.9 Hz, 1H), 7.70 (d, J=8.4 Hz, 1H), 7.48 (dt, J=24.5, 7.7 Hz, 1H), 7.42-7.30 (m, 1H), 4.50 (t, J=10.7 Hz, 1H), 4.33-4.24 (m, 1H), 4.24-4.10 (m, 1H), 4.02 (t, J=9.9 Hz, 1H), 3.93 (d, J=10.8 Hz, 1H), 3.47 (t, J=10.9 Hz, 1H), 3.42-3.29 (m, 2H), 3.14-2.78 (m, 2H) 3.13 (s, 2H), 2.47-1.92 (m, 2H), 1.58 (s, 9H). .sup.13C NMR (126 MHz, CDCl.sub.3) ? (ppm)=154.1, 152.5, 148.5, 141.4, 130.2, 127.7, 124.4, 122.7, 122.5, 120.3, 109.4, 81.5, 56.2, 53.1, 46.4, 41.8, 36.1, 33.8, 30.1, 28.6, 27.2.
Compounds of the Invention: Therapeutic Prodrugs
Me-SeS60-CBI-API (P8)
[0523] ##STR00106##
[0524] An oven-dried round-bottom flask was charged with X30 (41 mg, 0.074 mmol, 1.0 equiv.) and the compound was dissolved in anhydrous DCM (0.05 M, 1.5 mL). The solution was cooled to 0? C. and a solution of HCl (1.5 mL, 4 M in dioxane, 6.0 mmol, 80 equiv.) was added in one portion. The mixture was stirred for 0.5 h, was then allowed to warm to r.t. and was further stirred for 4 h until quantitative turnover was confirmed. All volatile compounds were removed under reduced pressure to afford the solid amine hydrochloride without further purification. An oven-dried round-bottom flask was charged with the amine hydrochloride and the compound was dissolved in anhydrous DMF (0.03 M). The solution was cooled to 0? C. and 5-(3-(azetidine-1-yl)propoxy-1H-indole-2-carboxylic acid (X29, 35.7 mg, 68% w/w, 0.088 mmol, 1.1 equiv.), EDCI (4 eq) and p-TsOH (1.1 eq) were added sequentially as solids. The mixture was stirred for 0.5 h, was then allowed to warm to r.t. and was further stirred for 15 h until quantitative turnover was confirmed. All volatile compounds were removed under reduced pressure to afford the crude mixture as a coloured solid. Purification by FCC (DCM/MeOH) gave the desired final amide as a yellow solid. Optional purification using a preparative HPLC system (H.sub.2O/MeCN/HCO.sub.2H) gave the title compound P8 as a colourless solid (27 mg, 0.038 mmol, 51%).
[0525] TLC R.sub.f=0.57 (DCM/MeOH, 4:1). NMR experiments at r.t. showed two rotameric speciesonly signals for the major rotamer are reported. .sup.1H NMR (400 MHz, DMSO-d.sub.6) ? (ppm)=11.64 (s, 1H), 9.86 (s, 1H), 8.21 (s, 1H), 8.04 (d, J=8.5 Hz, 1H), 7.84 (d, J=8.8 Hz, 1H), 7.61 (q, J=7.3 Hz, 1H), 7.52 (t, J=7.8 Hz, 1H), 7.47 (d, J=7.9 Hz, 1H, TsO.sup.?), 7.43 (d, J=9.0 Hz, 1H), 7.18 (s, 1H), 7.14 (s, 1H), 7.11 (d, J=8.0 Hz, 1H, TsO.sup.?), 6.95 (dd, J=8.9, 2.6 Hz, 1H), 4.96-4.78 (m, 2H), 4.61 (d, J=9.8 Hz, 1H), 4.41 (s, 1H), 4.33-4.22 (m, 1H), 4.14-3.97 (m, 6H), 3.56-3.39 (m, 1H), 3.25-2.95 (m, 1H), 3.15 (s, 2H), 2.41-2.30 (m, 2H), 2.28 (s, 1H, TsO.sup.?), 2.23-2.00 (m, 2H), 1.95 (p, J=6.5 Hz, 2H). .sup.13C NMR (126 MHz, DMSO-d.sub.6) ? (ppm)=160.2, 152.7, 150.7, 147.3, 145.8 (TsO.sup.?), 142.8, 141.4, 137.5, 135.6 (TsO.sup.?), 131.8, 130.8, 129.5, 128.0 (TsO.sup.?), 127.7, 127.5, 125.5 (TsO.sup.?), 124.3, 123.7, 122.4, 117.3, 115.9, 113.3, 110.4, 105.5, 103.4, 64.9, 56.0, 54.8, 54.0, 51.8, 47.2, 41.1, 32.9, 30.0, 26.6, 24.3, 20.8 (TsO.sup.?), 15.9.
5. Biological Testing
[0526] 5.1 Challenge with GR/GSH/Grx System Reductants (See
[0527] This cell-free assay examined the stability of dichalcogenide-based reduction-triggered compounds of the invention when exposed to cellular monothiol reductant GSH, and its cellular partner reductants glutathione reductase (GR) and glutaredoxins (Grx1 and Grx2).
[0528] Assay Conditions: A black 96-well plate with black bottom was charged with solutions of fluorophore-releasing reduction-triggered probes that are compounds of the invention (P1, P2, P3, P6 and P7) or that are not compounds of the invention and reveal the performance of prior art systems (disulfide X26), reaching 100 ?L final volume, 1% final DMSO, 10 ?M probe, aq. TE buffer (pH 7.4), with the indicated concentrations of reductant (in
[0529] Data Representation: F.sup.TCEP is the maximum possible fluorescence increase, corresponding to complete reduction-triggered cargo release by the TCEP incubation. F/F.sup.TCEP thus gives the fluorescence intensity, as a fraction of the maximum possible fluorescence signal, at the indicated times.
[0530] Experimental Results and Discussion: In general,
[0531] 5.2 Enzymatic Activation Assay (See
[0532] This cell-free assay (see
[0533] Assay conditions and data representation: A black 96-well plate with black bottom was charged with solutions of probes (P1, P2, P3, P6, P7) to reach final reaction conditions: 100 ?L of aq. TE buffer (pH 7.4), 10 ?M probe concentration, 1 vol % DMSO, and 20 nM of human recombinant thioredoxin reductase 1 (TrxR1; in
[0534] Experimental Results and Discussion: In general,
[0535] 5.3 Cellular Reduction Assay (See
[0536] Assay Conditions: Cells were cultured under standard conditions (Gao, L. et al. Cell Chemical Biology 2021, 28, 1-14. https://doi.org/10.1016/j.chembiol.2020.11.007), left to adhere for 24 h, then compounds were added to 100 ?M with 1% vol DMSO. Fluorescence timecourse measurements were acquired as in the chemoreductant assay, with all conditions in triplicates.
[0537] Data Representation: F.I.(t) is the fluorescence intensity signal increase as compared to time zero signal (fluorescence intensity immediately following compound addition, before probe activation could occur), in arbitrary units although with the same detection settings for all probes releasing the same cargo. This corresponds to reduction-triggered cargo release.
[0538] Experimental Results and Discussion:
[0539]
[0540]
[0541]
[0542] 5.4 In Vitro Cell Viability Assay (See
[0543] Assay Conditions: HeLa cells were cultured under standard conditions (Gao, L. et al. Cell Chemical Biology 2021, 28, 1-14. https://doi.org/10.1016/j.chembiol.2020.11.007), left to adhere for 24 h, then compounds were added from DMSO stock solution and adjusted to 1% vol DMSO. Cells were further incubated for 48 h, then 0.20 volume equivalents of a solution of resazurin in PBS (0.15 mg/mL) were added and incubated for 3 hours, then fluorescence (ex 544 nm, em 590 nm) was detected on a fluorescence platereader. Cell viability (%) is calculated from the fluorescence values, relative to a control experiment with no compound added.
[0544] Experimental Results and Discussion:
[0545] RX1 Enables Quantitative High-Throughput Cellular Screening for TrxR1 Inhibitors
[0546] Inhibitors of TrxR1 hold promise as therapeutics for treating cancer, autoimmune and inflammatory diseases. The chalcophilic gold complex auranofin (Ridaura) is one effective though poorly selective TrxR inhibitor, clinically used against the autoimmune inflammatory disease, rheumatoid arthritis. It and many analogue complexes have reached late-stage clinical trials in cancer, motivated by tumoral upregulation and reliance upon redox systems (Abdalbari, F. H. et al., Discover Oncology 12, 42 (2021)). Other TrxR inhibitor classes include redox-active species and organic electrophiles. Until now, TrxR1 assays to guide inhibitor development were enzymatic (cell-free) or utilised cell lysates. The technical and cost challenges of expressing and purifying mTrxR with near-quantitative selenium-incorporation on a sufficient scale for large screening, have limited enzymatic TrxR screening: only one high-throughput screen (HTS) has been reported (Stafford, W. C. et al., Sci. Transl. Med. 10, eaaf7444 (2018); Prast-Nielsen, S. et al., Free Radical Biology and Medicine 50, 1114-1123 (2011)). Enzymatic or lysate assay hits can be irrelevant in cells (poor permeability, biolocalisation, or target specificity), report artifactual hits (e.g. fluorescence quenching or aggregation), and can identify promiscuous compounds that are unlikely to be useful in biology, rather than selective compounds. Biochemical assays also cannot identify compounds that are biotransformed into active inhibitors: a field that is recently emerging for selenoprotein targeting (Eaton, J. K. et al., Nature Chemical Biology 16, 497-506 (2020)).
[0547] Cellular HTS can be far simpler and cheaper to perform; and allows screening different cell lines, towards therapeutic TrxR inhibitors effective in target cells (with varied expression levels of TrxR and of likely off-target thiol/selenol species), while controlling for drug-relevant performance issues such as upregulated electrophile detoxification and drug efflux pumping, and cell-type-dependent uptake. If a selective probe/readout is used, the data can also be more likely to emphasise selectivity over nonspecific reactivity, making it more actionable in drug development. A HTS-suitable probe must be TrxR-selective; but it must also pass many additional criteria for automatic operation, including (a) broad dynamic range (negligible background and cellular crosstalk, high signal-to-noise); (b) broad linear range (for quantitative use); (c) minimal count of error-prone steps (no additional reagents or handling); (d) no manual tailoring of conditions or processing by compound classes; (e) high-quality data: stable signal, with high precision (small deviations) and high accuracy (confidence of TrxR quantification, minimum interference from test compounds).
[0548] The stability and constant environment of P6's signal (crystallising fluorophore, protected from interferences), its low background (ESIPT quenched probe) and low crosstalk (high Stokes shift), and its ability to directly generate a readout, were promising features. We therefore set out to develop the first cellular quantitative HTS (qHTS) assay for TrxR1 inhibitors, using P6, and performing pilot screening with the 1280-compound LOPAC.sub.1280 library. LOPAC is intended to cover drugs and drug-like scaffolds with much comparative data on target selectivity, potency, cellular and in vivo bioactivity, without focus on any particular mechanism of action. The LOPAC library was used in the only previously reported qHTS enzymatic TrxR assay (in 2011), and there is 82% overlap in composition between the 2011 and 2021 version (Prast-Nielsen, S. et al., Free Radical Biology and Medicine 50, 1114-1123 (2011)).
[0549] Cellular screening using P6, optimized for HTS with 1536-well plates (assay volume 6 ?L, 500 cells/well) over 4 h run-time, reproduced the strong performance seen in 96-well format. Untreated vs no-cell controls gave a 7-fold raw signal to background ratio [S:B] without background compensation, with a high Z value of 0.64, suitable for HTS (
[0550] A LOPAC.sub.1280 screen using this assay protocol performed well (Z=0.63, S:B 7:1). Compounds with apparent toxicity within the assay time were excluded by a separate viability counter-assay. Moderate cut-off criteria (IC.sub.50<20 ?M and curve form) gave a <1.5% hit rate (18 of 1278 compounds, plus TRi-1 and TRi-2;
RX1 Enables Comparing Cellular to Cell-Free Potencies, which May Orient the Development of Effective and Cellularly-Selective TrxR1 Inhibitors
[0551] One opportunity afforded by P6-enabled cellular screening is to compare, for the first time, cell-free to cellular TrxR inhibition by species, to assess trends for cellularly-useful or -less-useful inhibitors. 1047 LOPAC compounds were assessed across both the screens (Prast-Nielsen, S. et al., Free Radical Biology and Medicine 50, 1114-1123 (2011)), of which 1011 generated robust enough data quality for comparisons. Of these, 993 compounds were inactive in both assays, and 7 were active in both. This 99% overlap of conclusive results between P6 and the purified enzyme screen speaks strongly to the precision of the cellular P6 assay. Only 2 compounds, IPA-3 and chloro-APB, were active in the cellular screen despite inactivity in the enzymatic assay: and these are catechol-like species with plausible redox activity in cells. To us, this indicates that the cellular P6 assay has excellent robustness against false positives. Given that hits were classed up to 20 ?M, while P6 relies on precipitation of >1 ?M of a flat aryl fluorophore for signal generation, we had feared false positive inhibition from the many LOPAC PAINS (pan-assay interference compounds) that have aggregation effects, or could alkylate the reduced intermediate C. However, not even powerful cellular PAINS rottlerin, rhodanine and myricetin (Plemper, R. K. et al., PLOS Pathogens 14, e1007038 (2018)) gave apparent potencies above 3% of that of the reference inhibitor TRi-1 (
[0552] Interestingly, the ratios of cell-free to cellular potencies of the shared hits clustered with their reactivity (
[0553] In conclusion, these strong results give confidence that cellular qHTS with P6 is a valid and valuable TrxR inhibitor screening strategy, promising that focused libraries can be used with cell lines of choice to guide development of cellularly-acting TrxR inhibitors.
[0554] 5.6 Summary
[0555] The compounds of the invention can have the unique feature of cellularly-selectively releasing their cargos under the activity of the key oxidoreductase TrxR1; they have comparable results across a range of cell lines; they are stable to hydrolysis; and they are entirely, or nearly entirely, stable to monothiol background from high levels of glutathione or monothiols which enables them to be cellularly-selective for redox-active proteins. Therefore the compounds of the invention can be selective probes or prodrugs to release a signal or a cargo such as a drug conditional upon the activity of a specific oxidoreductase or redox-active protein, a highly valuable feature for diagnostics and for prodrugs depending on the cargo used and the oxidoreductase or redox-active protein targeted.