Stabilized rice aleurone and preparation method thereof
11980216 ยท 2024-05-14
Assignee
- JIANGSU STATE FARM CEREALS INDUSTRY GROUP CO., LTD. (Nanjing, CN)
- Jiangnan University (Wuxi, CN)
- JIANGSU PROVINCIAL AGRICULTURAL RECLAMATION AND DEVELOPMENT CO., LTD. (Nanjing, CN)
Inventors
- Li Wang (Wuxi, CN)
- Hui LU (Nanjing, CN)
- Zhicun XU (Nanjing, CN)
- Zhengxing CHEN (Wuxi, CN)
- Zhaoqin ZONG (Nanjing, CN)
- Yongfu LI (Wuxi, CN)
- Xiaoyu FENG (Nanjing, CN)
- Jie JIANG (Wuxi, CN)
- Congnan ZHANG (Nanjing, CN)
- Haifeng Xu (Nanjing, CN)
- Ru Feng (Nanjing, CN)
Cpc classification
B02C23/20
PERFORMING OPERATIONS; TRANSPORTING
B02B3/00
PERFORMING OPERATIONS; TRANSPORTING
Y02P60/87
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
B07B4/025
PERFORMING OPERATIONS; TRANSPORTING
International classification
A23L5/20
HUMAN NECESSITIES
B02C23/20
PERFORMING OPERATIONS; TRANSPORTING
Abstract
The disclosure discloses stabilized rice aleurone and a preparation method, using the technology of classifying a rice aleurone layer by a tangential injection airflow impeller classifier combined with an ultrasonic vibrating screen for the first time, and simultaneously uses steam treatment coupled with thermal enzyme inactivation by drum drying as a stabilization method to prepare the stabilized rice aleurone for the first time. The drum drying equipment realizes the two purposes of enzyme inactivation and drying, and the method has the advantages of short process flow, simple equipment, high utilization rate, small floor space, low investment, low energy consumption and no pollution, and is suitable for industrial promotion. The stabilized rice aleurone has high nutritional value, is rich in functional ingredients, and can be used as a raw material for functional food development in industrial production and food applications.
Claims
1. A method for preparing rice aleurone, comprising the following steps: (1) after rice hulling and mechanical rice milling of paddy, obtaining rice and rice bran; classifying the rice bran into an aleurone component and a non-aleurone component by an air classifier, and further sieving and classifying the aleurone component to obtain crude rice aleurone; (2) mixing the crude rice aleurone obtained in step (1) with water to obtain a crude rice aleurone mixture, and subjecting the crude rice aleurone mixture to a steam treatment; (3) after the steam treatment, stirring the crude rice aleurone mixture with more water to obtain a rice aleurone slurry; and (4) adding the rice aleurone slurry to a drum scraper dryer, drying the rice aleurone slurry to obtain a dried rice aleurone, and then crushing the dried rice aleurone to obtain a rice aleurone product; wherein the aleurone component is further sieved and classified using an ultrasonic vibrating screen in the step (1), and a mesh number of the ultrasonic vibrating screen is in a range of 60-80 meshes; an amount of the water added is 5%-15% of mass of the crude rice aleurone in step (2); during the steam treatment, the crude rice aleurone mixture is distributed to a thickness of 1-5 cm in the step (2); a steam temperature during the steam treatment is controlled at 95? C.-120? C., and steam treatment time is 20-40 min in the step (2); a mass concentration of the rice aleurone slurry is 25%-50% in step (3); a steam pressure of the drum scraper dryer is 0.6-0.8 Mpa in step (4); and a motor speed of the drum scraper dryer is 50-300 rpm in the step (4).
2. A method for preparing rice aleurone, comprising the following steps: (1) after rice hulling and mechanical rice milling of paddy, obtaining rice and rice bran; classifying the rice bran into an aleurone component and a non-aleurone component by an air classifier, and further sieving and classifying the aleurone component to obtain crude rice aleurone; (2) mixing the crude rice aleurone obtained in step (1) with water to obtain a crude rice aleurone mixture, and subjecting the crude rice aleurone mixture to a steam treatment; (3) after the steam treatment, stirring the crude rice aleurone mixture with more water to obtain a rice aleurone slurry; and (4) adding the rice aleurone slurry to a drum scraper dryer, drying the rice aleurone slurry to obtain a dried rice aleurone, and then crushing the dried rice aleurone to obtain a rice aleurone product.
3. The method of claim 2, wherein the mechanical milling comprises milling by an emery roll rice mill process and/or an iron roll rice mill process in the step (1).
4. The method of claim 2, wherein the air classifier is a tangential injection airflow impeller pneumatic classifier.
5. The method of claim 4, wherein the tangential injection airflow impeller pneumatic classifier comprises tangential single-hole air injection or multi-hole air injection.
6. The method of claim 2, wherein the aleurone component is further sieved and classified using an ultrasonic vibrating screen in the step (1).
7. The method of claim 6, wherein a mesh number of the ultrasonic vibrating screen is in a range of 60-80 meshes.
8. The method of claim 2, wherein an amount of the water added is 5%-15% of mass of the crude rice aleurone in step (2).
9. The method of claim 2, wherein the steam treatment is to distribute the crude rice aleurone mixture to a thickness of 1-5 cm in step (2).
10. The method of claim 2, wherein a steam temperature during the steam treatment is controlled at 95? C.-120? C., and steam treatment time is 20-40 min in step (2).
11. The method of claim 2, wherein a mass concentration of the rice aleurone slurry is 25%-50% in step (3).
12. The method of claim 2, wherein the stirring further comprises a colloid milling treatment to obtain a more delicate rice aleurone slurry in step (3).
13. The method of claim 2, wherein a steam pressure of the drum scraper dryer is 0.6-0.8 Mpa in step (4).
14. The method of claim 2, wherein a motor speed of the drum scraper dryer is 50-300 rpm in step (4).
15. The method of claim 2, wherein a pulverization method adopted in step (4) is ultrafine pulverization, and a pulverization mesh number is 100-300 meshes.
Description
BRIEF DESCRIPTION OF FIGURES
(1)
(2)
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DETAILED DESCRIPTION
(13) The technical solution of the disclosure will be described in detail below with reference to the drawings and specific embodiments:
Example 1
(14) (1)
(15) In the process of rice processing, after hulling, paddy entered the mechanical rice milling process, the rice milling process adopts a process with three emery rolls and one iron roll, and the brown rice was milled by 3 vertical emery roll rice mills and 1 iron roll rice mill. After mechanical milling, the rice bran component was separated at fan equipment (No. 2 fan unit, purchased from Shanghai Xingying Electromechanical Co., Ltd., model 9-19).
(16) (2) The rice bran component obtained in step (1) was pneumatically conveyed to a tangential injection airflow impeller classifier (purchased from Shandong Dalier Heavy Industry Co., Ltd., model AF315-6) to perform aleurone component separation to obtain an aleurone component mainly including an aleurone layer and a non-aleurone component mainly including a non-aleurone layer.
(17) (3) The aleurone component obtained in step (2) was further classified using an ultrasonic vibrating screen (purchased from Xinxiang Dayong Vibration Equipment Co., Ltd., model DYC2000) to remove the remaining part of rice grist. The mesh number of the screen is 60 meshes, and the component under the screen is collected to obtain rice aleurone.
(18) (4) Clean domestic drinking water was sprayed into the rice aleurone obtained in step (3) in a mixer (purchased from Nanjing Kedixin Machinery Equipment Co., Ltd., model CH-200) while stirring is performed, and the amount of water added is 10% of the rice aleurone. The rice aleurone was uniformly mixed quickly in the mixer to ensure that the water was evenly distributed in the rice aleurone. The purpose of adding water in the step is to enhance heat transfer.
(19) (5) The rice aleurone after water was added in step (4) was conveyed into steam treatment equipment (purchased from Shandong Kingdealer Industrial Equipment Co., Ltd., model KD-ZX) by a conveyor belt. The distribution thickness of the rice aleurone on the conveyor belt is controlled to be 3 cm, the steam temperature is controlled at 100? C., and the steam treatment time is 20 min.
(20) (6) The rice aleurone after the steam treatment obtained in step (5) was automatically discharged to a stirring tank through a conveyor belt, water was added to the stirring tank and the slurry was quickly beaten. The concentration of the rice aleurone slurry was controlled at 30%.
(21) (7) The rice aleurone slurry obtained in step (6) was treated by colloidal milling, and then conveyed to drum scraper drying equipment (purchased from Dongtai Food Machinery Factory Co., Ltd., model HG-1800A) by a pump to perform drying. The steam pressure was controlled at 0.65 Mpa, the motor speed was controlled at 300 rpm, and the dried material was collected.
(22) (8) The dried material collected in step (7) was pulverized to 100-300 meshes by an ultrafine pulverizer (purchased from Jiangyin Shengtian Machine Manufacturing Co., Ltd., model STWF-60), and packaged to obtain the stabilized rice aleurone.
(23) After detection, the stabilized rice aleurone obtained by the method has a free fatty acid value of 23.7 mg KOH/100 g, a lipase inactivation rate of 85% or more, a peroxidase inactivation rate of 100%, and a shelf life of 12 months or more (as shown in
(24) After detection, the stabilized rice aleurone obtained by the method has a total number of colonies of 26 CFU/g, coliform bacteria were not detected, molds and yeasts were not detected, pathogens were not detected, aflatoxin B1 was not detected, and ochratoxin was not detected. The taste is pure, and the health and safety indexes meet the national food standards (as shown in Table 1).
(25) The stabilized rice aleurone prepared by the method is analyzed for nutritional functional components. The product is rich in protein, dietary fiber, B vitamins, vitamin E, phenolic acids, mineral elements and other functional components (as shown in Table 2). Moreover, the stabilization treatment has little effect on the nutritional functional components, and the stabilized rice aleurone can be used as a raw material for functional food development.
(26) TABLE-US-00001 TABLE 1 Detection results of safety indexes of rice aleurone Safety indexes Detection results Total number of colonies <100 CFU/g Coliform bacteria Not detected Mold and yeast Not detected Aflatoxin B1 Not detected Ochratoxin A Not detected
(27) TABLE-US-00002 TABLE 2 Nutrient content of rice aleurone of Example 1 and comparison with polished rice Ratio of Content Content in content in in non- stabilized Content in stabilized rice stabilized rice polished aleurone and rice Nutrient aleurone rice polished rice aleurone Protein/% 16.2 7.5 2.2:1 15.7 Fat/% 7.4 0.7 10.6:1 7.2 Starch/% 63.2 70.1 0.9:1 64.3 Dietary fiber/% 10.1 0.7 14.4:1 9.6 Vitamin B1/(mg/kg) 11.0 1.4 7.9:1 11.2 Vitamin B2/(mg/kg) 1.2 0.5 2.4:1 1.1 Vitamin E/(mg/kg) 5 / / 4.8 GABA/(mg/kg) 599.2 299.5 2.0:1 594.7 Phytic acid/(g/kg) 14.5 1.2 12.1:1 13.8 Ferulic acid/(mg/kg) 1262.7 124.6 10.2:1 1247.9 P-Coumaric 231.8 / / 210.7 acid/(mg/kg) Phosphorus/(mg/kg) 13371.6 1230.2 10.9:1 13156.0 Calcium/(mg/kg) 527.3 40.1 13.2:1 531.4 Magnesium/(mg/kg) 6736.4 200.2 33.6:1 6802.0 Iron/(mg/kg) 61.4 7.1 8.7:1 55.8 Zinc/(mg/kg) 36.9 9.7 3.8:1 33.5 Copper/(mg/kg) 18.4 2.6 7.1:1 17.7 Manganese/(mg/kg) 107.1 10.7 .sup.10:01 97.4
(28) wherein the polished rice refers to commercially available pre-packaged rice.
(29) Shelf Life Experiment (Accelerated Test):
(30) The product was cultured in a constant temperature and humidity incubator at 50? C. and a relative humidity of 60% for 12 weeks, and the lipase activity, peroxidase activity and fatty acid value of the product were regularly tested. The results are shown in
(31) In the stabilized rice aleurone of the present example, the peroxidase inactivation rate is 100%, and the lipase inactivation rate is 85% or more. After 12 weeks of culture in a constant temperature and humidity incubator at 50? C. and a relative humidity of 60%, the free fatty acid value of the stabilized rice aleurone is always stable below 50 mg KOH/100 g (as shown in
(32) Storage at 50? C. and a relative humidity of 60% for 1 week is equivalent to storage at room temperature (23-35? C.) for 1 month. That is, the product of the example can be stored at room temperature for 12 months without being rancid.
(33) After untreated rice aleurone (the aleurone obtained in step 1) was stored at 50? C. and a relative humidity of 60% for 12 weeks, the color of the rice aleurone was found to darken (as shown in the right picture in
Example 2
Process Optimization
(34) 1) Selection of the Amount of Water Added in Step (2):
(35) 5 parts of the rice aleurone obtained in step (3) of Example 1 were taken, and 2 wt %, 5 wt %, 10 wt %, 15 wt % and 20 wt % water was added according to the mass percentage respectively, and the rice aleurone and water were mixed uniformly. The uniformly mixed rice aleurone was spread on steamer trays to a thickness of 3 cm, and steamed at 100? C. for 20 min. The residue of the lipase and peroxidase activity was detected respectively, and the results are shown in
(36) The addition of water to rice aleurone can enhance heat transfer, thereby improving the enzyme inactivation effect and reducing energy consumption costs. Insufficient water addition cannot achieve the heat transfer enhancing effect. Too much water addition will cause the viscosity of the sample to increase, the heat transfer effect will deteriorate, the enzyme inactivation effect will deteriorate, and the product will become discolored.
(37) It can be seen from
(38) 2) Selection of Thickness of Rice Aleurone During Steam Treatment in Step (2):
(39) 5 parts of the rice aleurone obtained in step (3) of Example 1 were taken, 10 wt % clean domestic drinking water was sprayed according to the mass percentage, and the rice aleurone and water were mixed uniformly. The uniformly mixed rice aleurone was spread on steamer trays to a thickness of 2 cm, 3 cm, 4 cm, 5 cm and 6 cm, and steamed at 100? C. for 20 min. The residue of the lipase and peroxidase activity was detected respectively, and the results are shown in
(40) It can be seen from
(41) 3) Selection of Temperature During Steam Treatment in Step (2):
(42) 5 parts of the rice aleurone obtained in step (3) of Example 1 were taken, 10 wt % clean domestic drinking water was sprayed according to the mass percentage, and the rice aleurone and water were mixed uniformly. The uniformly mixed rice aleurone was spread on steamer trays to a thickness of 3 cm, and steamed at 90? C., 100? C., 105? C. and 110? C. for 20 min respectively. The residue of the lipase and peroxidase activity was detected respectively, and the results are shown in
(43) The higher the steam temperature, the stronger the heat penetration, and theoretically the better the enzyme inactivation effect. However, high-temperature and high-pressure steam can also cause the Maillard reaction of the rice aleurone to cause discoloration of the product, which is not conducive to product sales. At the same time, high temperature and high pressure have high equipment requirement and high cost.
(44) It can be seen from
(45) 4) Selection of Time in Steam Treatment in Step (2):
(46) 5 parts of the rice aleurone obtained in step (3) of Example 1 were taken, 10 wt % clean domestic drinking water was sprayed according to the mass percentage, and the rice aleurone and water were mixed uniformly. The uniformly mixed rice aleurone was uniformly spread on steamer trays to a thickness of 3 cm respectively, and treated at 100? C. for 10 min, 15 min, 20 min, 25 min and 30 min respectively. The residue of the lipase and peroxidase activity was detected respectively, and the results are shown in
(47) The longer the steam treatment time, theoretically the better the enzyme inactivation effect. However, if the time is too long, on the one hand, discoloration of the rice aleurone product will be caused, which is not conducive to product sales, and on the other hand, high process cost will be caused.
(48) It can be seen from
(49) 4-Factor 3-Level Orthogonal Experiments Were Performed on the Amount of Water Added, Thickness, Temperature and Time to Determine the Most Preferred Solution of Steam Treatment. The Results are Shown in Table 3.
(50) TABLE-US-00003 TABLE 3 Effects of different factors on steam treatment of rice aleurone A Results Amount B C D Lipase Peroxidase of water Thickness/ Temperature/ Time/ inactivation inactivation Sensory Overall No. added/% cm ? C. min rate/% rate/% quality ratings 1 5.0 3.0 95.0 15.0 35.20% 43.50% 5.5 2 5.0 4.0 100.0 20.0 47.60% 76.70% 7.3 3 5.0 5.0 105.0 25.0 42.50% 64.70% Color 5.7 damaged 4 10.0 3.0 100.0 25.0 58.60% 81.30% 9.1 5 10.0 4.0 105.0 15.0 51.50% 75.30% Color 8.2 damaged 6 10.0 5.0 95.0 20.0 29.70% 36.40% 5.3 7 15.0 3.0 105.0 20.0 47.90% 65.40% Color 7.2 damaged 8 15.0 4.0 95.0 25.0 28.80% 35.20% 6.2 9 15.0 5.0 100.0 15.0 46.40% 61.50% 7.1 K1 18.50 21.80 17.00 20.80 K2 22.60 21.70 23.50 19.80 K3 20.50 18.10 21.10 21.00 k1 6.17 7.27 5.67 6.93 k2 7.53 7.23 7.83 7.70 k3 6.83 6.03 7.03 7.00 R 1.37 1.23 2.17 0.77 Priority Temperature-Amount of water added-Thickness-Time order of factors Most C2A2B1D2 preferred solution
(51) wherein the calculation method of the overall rating is: the overall rating is obtained by the enzyme inactivation effect rating plus the sensory evaluation rating: overall rating=lipase inactivation rate?5+peroxidase inactivation rate?5+sensory evaluation rating.
(52) A2 refers to the level 2 of A factor, which is 10%, and so on.
(53) Combining
(54) 5) Selection of Parameters of the Drum Scraper Dryer in Step (4):
(55) Selection of motor speed parameter:
(56) 5 parts of rice aleurone after steam treatment by the most preferred solution were taken, 10 wt % clean domestic drinking water was sprayed according to the mass percentage, and the rice aleurone and water were mixed uniformly. The uniformly mixed rice aleurone was uniformly spread on steamer trays to a thickness of 3 cm respectively, and treated at 100? C. for 20 min respectively.
(57) Water was added respectively to prepare slurry, and the slurry concentration was controlled at about 35%. After colloid milling, the slurry was dried in a drum at the steam pressure of 0.7 MPa and the motor speeds of 100 rpm, 200 rpm, 300 rpm, 400 rpm and 500 rpm respectively. Samples were collected, and the residue of lipase and peroxidase activity was detected respectively. The results are shown in
(58) It can be seen from the results in
(59) Selection of steam pressure parameters of the drum scraper dryer:
(60) 5 parts of rice aleurone after steam treatment by the most preferred solution were taken, 10 wt % clean domestic drinking water was sprayed according to the mass percentage, and the rice aleurone and water were mixed uniformly. The uniformly mixed rice aleurone was uniformly spread on steamer trays to a thickness of 3 cm respectively, and treated at 100? C. for 20 min respectively.
(61) Water was added respectively to prepare slurry, and the slurry concentration was controlled at about 35%. After colloid milling, the slurry was dried in a drum at the motor speed of 300 rpm and steam pressure of 0.55 MPa, 0.6 MPa, 0.65 MPa, 0.7 MPa, and 0.75 MPa respectively. Samples were collected, and the residue of lipase and peroxidase activity was detected respectively. The results are shown in
(62) It can be seen from the results in
(63) 6) Selection of Sieving Mesh Number in Step (3):
(64) Phytic acid is one of the iconic components of the aleurone layer. The higher the purity of the aleurone layer in rice aleurone, the higher the content of phytic acid. In the present example, the mesh number of the vibrating screen was comprehensively determined by the phytic acid content and the utilization rate of rice aleurone.
(65) Referring to Example 1, other conditions remained unchanged, only the mesh number of the ultrasonic vibrating screen in step (3) was replaced with 40, 60, 80 and 100 mesh screens for performing sieving. Rice aleurone was collected separately, and the phytic acid content and the aleurone yield were determined respectively. The results are shown in
(66) It can be seen from
(67) The disclosure uses the technology of classifying a rice aleurone layer by a tangential injection airflow impeller classifier combined with an ultrasonic vibrating screen for the first time, uses steam treatment coupled with thermal enzyme inactivation by drum drying as a stabilization method for the first time, and enables the drum drying equipment to realize two purposes of enzyme inactivation and drying. The disclosure has high utilization rate of the aleurone layer, stable product, short process flow, simple equipment, high utilization rate of equipment, small floor space, low investment, low energy consumption and no pollution, and is suitable for industrial promotion. By using the method of the disclosure to produce the stabilized rice aleurone, the shortcomings of poor enzyme inactivation stabilization effect, incomplete enzyme inactivation, and unstable product quality of a single process are overcome, the shortcomings of a twin-screw extrusion process that although the stabilization effect is good, the taste of the product is poor are overcome, and the taste and shelf life of the product are guaranteed.
(68) The stabilized rice aleurone produced by the technology of the disclosure has the characteristic of good stabilization effect and also the characteristic of high nutritional value, is rich in functional ingredients such as dietary fiber, B vitamins, vitamin E, phenolic acid and mineral elements, and can be used as a raw material for functional food development.