Humanized anti-CD45 antibodies

Abstract

Provided are humanized anti-human CD45 antibodies and pharmaceutical compositions including the antibodies.

Claims

1. An anti-huCD45 antibody or a huCD45-binding antibody fragment, comprising: (i) an immunoglobulin heavy chain variable region comprising SEQ ID NO:17, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:22, or SEQ ID NO:2; and (ii) an immunoglobulin light chain variable region comprising SEQ ID NO:43, SEQ ID NO:48, SEQ ID NO:33, SEQ ID NO:38, or SEQ ID NO:28, provided that when the heavy chain variable region comprises SEQ ID NO:2, the light chain variable region is not SEQ ID NO:28.

2. The anti-huCD45 antibody or huCD45-binding antibody fragment of claim 1, comprising: (i) an immunoglobulin heavy chain sequence comprising SEQ ID NO:21, SEQ ID NO:26, SEQ ID NO:11, SEQ ID NO:16, or SEQ ID NO:6; and (ii) an immunoglobulin light chain sequence comprising SEQ ID NO:47, SEQ ID NO:52, SEQ ID NO:37, SEQ ID NO:42, or SEQ ID NO:32.

3. An anti-huCD45 antibody or huCD45-binding antibody fragment, comprising: (a) a set of immunoglobulin heavy chain complementarity determining regions (CDRs) comprising heavy chain CDR1, CDR2 and CDR3 amino acid sequences selected from the following sets: (i) SEQ ID NO:53, SEQ ID NO:58, and SEQ ID NO:55, respectively, (ii) SEQ ID NO:53, SEQ ID NO:56, and SEQ ID NO:55, respectively, and (iii) SEQ ID NO:53, SEQ ID NO:57, and SEQ ID NO:55, respectively; and (b) a set of immunoglobulin light chain complementarity determining regions (CDRs) comprising light chain CDR1, CDR2 and CDR3 amino acid sequences selected from the following sets: (i) SEQ ID NO:59, SEQ ID NO:63, and SEQ ID NO:61, respectively, and (ii) SEQ ID NO:59, SEQ ID NO:64, and SEQ ID NO:61, respectively, and (iii) SEQ ID NO:62, SEQ ID NO:60, and SEQ ID NO:61, respectively.

4. A pharmaceutical composition comprising the antibody or antibody fragment of claim 1 and at least one pharmaceutically acceptable excipient.

5. A radiopharmaceutical composition comprising: the antibody or antibody fragment of claim 1; a radionuclide linked to the antibody or antibody fragment, and at least one pharmaceutically acceptable excipient.

6. The radiopharmaceutical composition of claim 5, wherein the radionuclide is an alpha particle emitter or a beta particle emitter.

7. The radiopharmaceutical composition of claim 5, wherein the radionuclide comprises .sup.131I.

8. The radiopharmaceutical composition of claim 5, wherein the radionuclide comprises .sup.225Ac, .sup.177Lu or .sup.90Y.

9. A composition comprising the antibody or antibody fragment of claim 1, chemically conjugated to a chelator.

10. The composition of claim 9, wherein the chelator comprises DOTA or a DOTA derivative.

11. The composition of claim 9, further comprising a radionuclide chelated by the chelator.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) Various antibody heavy and light chain amino acid sequences are set forth in FIGS. 1-10.

(2) FIG. 1 shows the amino acid sequence of a heavy chain of mouse anti-huCD45 antibody (m CD45-HC parental) that binds all isoforms of human CD45 (huCD45) and portions thereof used as the basis for producing humanized anti-huCD45 antibodies.

(3) FIG. 2 shows the amino acid sequence of a humanized anti-huCD45 antibody heavy chain (hu CD45.HC1) and portions thereof.

(4) FIG. 3 shows the amino acid sequence of a humanized anti-huCD45 antibody heavy chain (hu CD45.HC2) and portions thereof.

(5) FIG. 4 shows the amino acid sequence of a humanized anti-huCD45 antibody heavy chain (hu CD45.HC3) and portions thereof.

(6) FIG. 5 shows the amino acid sequence of a humanized anti-huCD45 antibody heavy chain (hu CD45.HC4) and portions thereof.

(7) FIG. 6 shows the amino acid sequence of a light chain of mouse anti-huCD45 antibody (m CD45-LC parental) and portions thereof used as the basis for producing humanized anti-huCD45 antibodies.

(8) FIG. 7 shows the amino acid sequence of a humanized anti-huCD45 antibody light chain (hu CD45.LC1) and portions thereof.

(9) FIG. 8 shows the amino acid sequence of a humanized anti-huCD45 antibody light chain (hu CD45.LC2) and portions thereof.

(10) FIG. 9 shows the amino acid sequence of a humanized anti-huCD45 antibody light chain (hu CD45.LC3) and portions thereof.

(11) FIG. 10 shows the amino acid sequence of a humanized anti-huCD45 antibody light chain (hu CD45.LC4) and portions thereof.

(12) FIG. 11 shows cell binding data for binding of various humanized anti-huCD45 antibodies disclosed herein to MV411 human AML cell line cells, versus controls.

(13) FIG. 12 shows cell binding data for binding of various humanized anti-huCD45 antibodies disclosed herein to U937 human pro-monocytic myeloid leukemia cell line cells, versus controls.

(14) FIG. 13 shows cell binding data for binding of various humanized anti-huCD45 antibodies disclosed herein to HL60 human, promyelocytic AML cell line cells, versus a modified chimeric parental control antibody.

(15) FIG. 14 shows cell viability data for HL60 cells treated with .sup.225Ac-labeled anti-huCD45 antibodies as disclosed herein, 225Ac-labeled parental antibody, or corresponding unlabeled control antibodies.

(16) The complementarity determining region (CDR) amino acid sequences are underlined in the presentation of the variable region sequences in the figures.

DETAILED DESCRIPTION

(17) One aspect of the invention provides an anti-(human CD45) [anti-huCD45] antibody, comprising: (i) a heavy chain which is mCD45-HC parental, huCD45.HC1, huCD45.HC2, huCD45.HC3, huCD45.HC1, or huCD45.HC4, or any of said heavy chains without the signal sequence, or a heavy chain comprising the heavy chain variable region of any of said heavy chains; and (ii) a light chain which is mCD45-LC parental, huCD45.LC1, huCD45.LC2, huCD45.LC3, or huCD45.LC4, or any of said light chains without the signal sequence, or a light chain comprising the light chain variable region of any of said light chains,
provided that when the heavy chain is mCD45-HC parental with or without the signal sequence, the light chain is not mCD45-LC parental with or without the signal sequence,
or a human CD45-binding fragment of the antibody, such as a Fab fragment or Fab.sub.2 fragment or a corresponding scFv molecule.

(18) The parental murine monoclonal antibody including the parental heavy chain shown in FIG. 1 and the parental light chain shown in FIG. 6, which binds all isoforms of human CD45, was used as the basis for designing humanized anti-huCD45 antibodies. Antibodies consisting of various combinations of the humanized immunoglobulin heavy chains and immunoglobulin light chains were expressed in CHO cells and isolated for analysis and comparison to the parental antibody. Binding experiments were performed using an Octet HTX (Sartorius, Bohemia, New York, USA) at 25? C. to determine the KD's of the antibodies. The antibodies were loaded onto anti-human Fc capture (AHC) sensors. The loaded sensors were dipped into serial dilutions of rhCD45 His tag (900 nM start, 1:3 dilution, 3 points; R&D Systems, Minneapolis, MN, USA). Reference sample well (buffer) was used for data analysis. Kinetic constants were calculated using a monovalent (1:1) binding model. Results are shown in Table 1 below.

(19) TABLE-US-00001 TABLE 1 Sample ID KD (M) m CD45 Parental 3.75E?08 hu CD45 HC2 + LC1 2.44E?07 hu CD45 HC2 + LC3 1.96E?07 hu CD45 HC2 + LC4 2.47E?07 hu CD45 HC3 + LC1 1.63E?07 hu CD45 HC3 + LC3 5.02E?08 hu CD45 HC3 + LC4 1.09E?07 hu CD45 HC4 + LC1 2.32E?07 hu CD45 HC4 + LC3 1.51E?07 hu CD45 HC4 + LC4 1.23E?07
hu CD45 HC3+LC3 retained similar binding affinity as the parental antibody while all other humanized samples showed less than 10-fold changes in KD values relative to the parental antibody.

(20) Another aspect of the invention provides an anti-huCD45 antibody or a human CD45-binding antibody fragment, including: (i) an immunoglobulin heavy chain variable region including SEQ ID NO:2 (from mCD45-HC parental), SEQ ID NO:7 (from huCD45.HC1), SEQ ID NO:12 (from huCD45.HC2), SEQ ID NO:17 (from huCD45.HC3), or SEQ ID NO:22 (from huCD45.HC4); and (ii) an immunoglobulin light chain variable region including SEQ ID NO:28 (from mCD45-LC parental), SEQ ID NO:33 (from huCD45.LC1), SEQ ID NO:38 (from huCD45.LC2), SEQ ID NO:43 (from huCD45.LC3), or SEQ ID NO:48 (from huCD45.LC4),
provided that when the heavy chain variable region includes SEQ ID NO:2, the light chain variable region is not SEQ ID NO:28.

(21) In a variation of the preceding aspect of the invention, the anti-huCD45 antibody or human CD45-binding antibody fragment, includes: (i) an immunoglobulin heavy chain sequence including SEQ ID NO:6 (from mCD45-HC parental), SEQ ID NO:11 (from huCD45.HC1), SEQ ID NO:16 (from huCD45.HC2), SEQ ID NO:21 (from huCD45.HC3), or SEQ ID NO:26 (from huCD45.HC4); and (ii) an immunoglobulin light chain sequence including SEQ ID NO:32 (from mCD45-LC parental), SEQ ID NO:37 (from huCD45.LC1), SEQ ID NO:42 (from huCD45.LC2), SEQ ID NO:47 (from huCD45.LC3), or SEQ ID NO:52 (from huCD45.LC4).

(22) A further aspect of the invention provides an anti-huCD45 antibody or a huCD45-binding antibody fragment, including: (a) a set of immunoglobulin heavy chain complementarity determining regions (CDRs) including heavy chain CDR1, CDR2 and CDR3 amino acid sequences selected from the following sets: (i) SEQ ID NO:53, SEQ ID NO:56, and SEQ ID NO:55, respectively, (ii) SEQ ID NO:53, SEQ ID NO:57, and SEQ ID NO:55, respectively, (iii) SEQ ID NO:53, SEQ ID NO:58, and SEQ ID NO:55, respectively, and (iv) SEQ ID NO:53, SEQ ID NO:54, and SEQ ID NO:55, respectively; and (b) a set of immunoglobulin light chain complementarity determining regions (CDRs) including light chain CDR1, CDR2 and CDR3 amino acid sequences selected from the following sets: (i) SEQ ID NO:62, SEQ ID NO:60, and SEQ ID NO:61, respectively, (ii) SEQ ID NO:59, SEQ ID NO:63, and SEQ ID NO:61, respectively, (iii) SEQ ID NO:59, SEQ ID NO:64, and SEQ ID NO:61, respectively, and. (iv) SEQ ID NO:59, SEQ ID NO:60, and SEQ ID NO:61,
provided that when the heavy chain CDR set is (a)(iv), the light chain CDR set is not (b)(iv). It should be understood that the antibody or antibody fragment includes a heavy chain variable region that includes the heavy chain CDRs and a light chain variable region that includes the light chain CDRs.

(23) A related aspect of the invention provides an anti-huCD45 antibody or a huCD45-binding antibody fragment, including: (a) a set of immunoglobulin heavy chain complementarity determining regions (CDRs) including heavy chain CDR1, CDR2 and CDR3 amino acid sequences selected from the following sets: (i) SEQ ID NO:53, SEQ ID NO:56, and SEQ ID NO:55, respectively, (ii) SEQ ID NO:53, SEQ ID NO:57, and SEQ ID NO:55, respectively, and (iii) SEQ ID NO:53, SEQ ID NO:58, and SEQ ID NO:55, respectively; and (b) a set of immunoglobulin light chain complementarity determining regions (CDRs) including light chain CDR1, CDR2 and CDR3 amino acid sequences selected from the following sets: (i) SEQ ID NO:62, SEQ ID NO:60, and SEQ ID NO:61, respectively, (ii) SEQ ID NO:59, SEQ ID NO:63, and SEQ ID NO:61, respectively, and (iii) SEQ ID NO:59, SEQ ID NO:64, and SEQ ID NO:61, respectively.
It should again be understood that the antibody or antibody fragment includes a heavy chain variable region that includes the heavy chain CDRs and a light chain variable region that includes the light chain CDRs.

(24) In a variation of either of the preceding two aspects of the invention or of other aspects of the invention in which CDR sequences are recited, the antibody or antibody fragment or protein further includes with respect to one or more of the recited CDRs, in any and all combinations, additional N-terminal and/or C-terminal amino acid sequence, of 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 amino acid residues immediately adjacent to the one or more CDRs (on either or both of the N-terminal and C-terminal side of the CDR(s)) as shown in the any of the respective variable region amino acid sequences set forth in FIGS. 1-10.

(25) Another aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45-binding antibody fragment (such as Fab fragment, Fab.sub.2 fragment, or scFv molecule) or an immunoglobulin light chain, including an antibody light chain variable region including the following light chain CDRs: a CDR-L1 including the amino acid sequence RASKSVSTSGYSYLH (SEQ ID NO:59), KSSKSVSTSGYSYLH (SEQ ID NO:62), RASKSVSTSGYSYLA (SEQ ID NO:65), RASKSVSTSGYSYLS (SEQ ID NO:66), or RASKSVSTSGYSYLN (SEQ ID NO:67); a CDR-L2 including the amino acid sequence LASNLES (SEQ ID NO: 60), LASNLA (SEQ ID NO:68), LASNLAT (SEQ ID NO:63), LASNLQ (SEQ ID NO:69), LASNLQS (SEQ ID NO:64), LASTRES (SEQ ID NO:70), LASTRAT (SEQ ID NO:71), LASNRAT (SEQ ID NO:72), or LASSQLS (SEQ ID NO:73); a CDR-L3 including the amino acid sequence QHSRELPFT (SEQ ID NO:61), provided that the combination of a CDR-L1 including the amino acid sequence RASKSVSTSGYSYLH (SEQ ID NO:59) and CDR-L2 including the amino acid sequence LASNLES (SEQ ID NO:60) is excluded.
The next amino acid residues following the CDR-L3 sequence QHSRELPFT (SEQ ID NO:61) in the protein may, for example, be FGQ.

(26) A further aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45-binding antibody fragment (such as Fab fragment, Fab.sub.2 fragment, or scFv molecule) or an immunoglobulin heavy chain, including an antibody heavy chain variable region including the following light chain CDRs: a CDR-H1 including the amino acid sequence GFDFSRYWMS (SEQ ID NO:53) or GFDFSRYWMN (SEQ ID NO:85); a CDR-H2 including the amino acid sequence EINPTSSTINFTPSLKD (SEQ ID NO:54), EINPTSSTINFADSVKG (SEQ ID NO:56), EINPTSSTINYADSVKG (SEQ ID NO:57), EINPTSSTINFVDSVKG (SEQ ID NO:58), YINPTSSTIYYADSVKG (SEQ ID NO:74), AINPTSSTIYYADSVKG (SEQ ID NO:75), NINPTSSTIYYVDSVKG (SEQ ID NO:76), or SINPTSSTIYYADSVKG (SEQ ID NO:77); a CDR-H3 including the amino acid sequence GNYYRYGDAMDY (SEQ ID NO:55),
provided that the combination of a CDR-H1 including the amino acid sequence GFDFSRYWMS (SEQ ID NO:53) and CDR-H2 including the amino acid sequence EINPTSSTINFTPSLKD (SEQ ID NO:54) is excluded.

(27) Still another aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45-binding antibody fragment (such as Fab fragment, Fab.sub.2 fragment, or scFv molecule), including: (a) an antibody light chain variable region including the following light chain CDRs: a CDR-L1 including the amino acid sequence RASKSVSTSGYSYLH (SEQ ID NO:59), KSSKSVSTSGYSYLH (SEQ ID NO:62), RASKSVSTSGYSYLA (SEQ ID NO:65), RASKSVSTSGYSYLS (SEQ ID NO:66), or RASKSVSTSGYSYLN (SEQ ID NO:67); a CDR-L2 including the amino acid sequence LASNLES (SEQ ID NO:60), LASNLA (SEQ ID NO:68), LASNLAT (SEQ ID NO:63), LASNLQ (SEQ ID NO:69), LASNLQS (SEQ ID NO:64), LASTRES (SEQ ID NO:70), LASTRAT (SEQ ID NO:71), LASNRAT (SEQ ID NO:72), or LASSQLS (SEQ ID NO:73); and a CDR-L3 including the amino acid sequence QHSRELPFT (SEQ ID NO:61); and (b) an antibody heavy chain variable region including the following heavy chain CDRs: a CDR-H1 including the amino acid sequence GFDFSRYWMS (SEQ ID NO:53) or GFDFSRYWMN (SEQ ID NO:85); a CDR-H2 including the amino acid sequence EINPTSSTINFTPSLKD (SEQ ID NO:54), EINPTSSTINFADSVKG (SEQ ID NO:56), EINPTSSTINYADSVKG (SEQ ID NO:57), EINPTSSTINFVDSVKG (SEQ ID NO:58), YINPTSSTIYYADSVKG (SEQ ID NO:74), AINPTSSTIYYADSVKG (SEQ ID NO:75), NINPTSSTIYYVDSVKG (SEQ ID NO:76), or SINPTSSTIYYADSVKG (SEQ ID NO:77); and a CDR-H3 including the amino acid sequence GNYYRYGDAMDY (SEQ ID NO:55),
provided that the combination of CDR-L1 including the amino acid sequence RASKSVSTSGYSYLH (SEQ ID NO:59), CDR-L2 including the amino acid sequence LASNLES (SEQ ID NO:60), CDR-L3 including the amino acid sequence QHSRELPFT (SEQ ID NO:61), CDR-H1 including the amino acid sequence GFDFSRYWMS (SEQ ID NO:53), CDR-H2 including the amino acid sequence EINPTSSTINFTPSLKD (SEQ ID NO:54), and CDR-H3 including the amino acid sequence GNYYRYGDAMDY (SEQ ID NO:55) is excluded. The next amino acid residues following the CDR-L3 sequence QHSRELPFT (SEQ ID NO:61) in the protein may, for example, be FGQ.

(28) A further aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45-binding antibody fragment (such as Fab fragment, Fab.sub.2 fragment, or scFv molecule) or an immunoglobulin light chain, including an antibody light chain variable region including the following light chain CDRs: a CDR-L1 including the amino acid sequence RASKSVSTSGYSYLH (SEQ ID NO:59) or KSSKSVSTSGYSYLH (SEQ ID NO:62); a CDR-L2 including the amino acid sequence LASNLA (SEQ ID NO:68), LASNLAT (SEQ ID NO:63), LASNLQ (SEQ ID NO:69), or LASNLQS (SEQ ID NO:64); and a CDR-L3 including the amino acid sequence QHSRELPFT (SEQ ID NO:61).
The next amino acid residues following the CDR-L3 sequence QHSRELPFT (SEQ ID NO:61) in the protein may, for example, be FGQ.

(29) Another aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45-binding antibody fragment (such as Fab fragment, Fab.sub.2 fragment, or scFv molecule) or an immunoglobulin heavy chain, including an antibody heavy chain variable region including the following heavy chain CDRs: a CDR-H1 including the amino acid sequence GFDFSRYWMS (SEQ ID NO:53); a CDR-H2 including the amino acid sequence EINPTSSTINFADSVKG (SEQ ID NO:56), EINPTSSTINYADSVKG (SEQ ID NO:57), or EINPTSSTINFVDSVKG (SEQ ID NO: 58); and a CDR-H3 including the amino acid sequence GNYYRYGDAMDY (SEQ ID NO:55).

(30) A still further aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45-binding antibody fragment (such as Fab fragment, Fab.sub.2 fragment, or scFv molecule), including: (a) an antibody light chain variable region including the following light chain CDRs: a CDR-L1 including the amino acid sequence RASKSVSTSGYSYLH (SEQ ID NO:59) or KSSKSVSTSGYSYLH (SEQ ID NO:62); a CDR-L2 including the amino acid sequence LASNLES (SEQ ID NO:60), LASNLA (SEQ ID NO:68), LASNLAT (SEQ ID NO:63), LASNLQ (SEQ ID NO:69), LASNLQS (SEQ ID NO:64); and a CDR-L3 including the amino acid sequence QHSRELPFT (SEQ ID NO:61); and (b) an antibody heavy chain variable region including the following heavy chain CDRs: a CDR-H1 including the amino acid sequence GFDFSRYWMS (SEQ ID NO:53); a CDR-H2 including the amino acid sequence EINPTSSTINFTPSLKD (SEQ ID NO:54), EINPTSSTINFADSVKG (SEQ ID NO:56), EINPTSSTINYADSVKG (SEQ ID NO:57), or EINPTSSTINFVDSVKG (SEQ ID NO:58); and a CDR-H3 including the amino acid sequence GNYYRYGDAMDY (SEQ ID NO:55),
provided that the combination of CDR-L1 including the amino acid sequence RASKSVSTSGYSYLH (SEQ ID NO:59), CDR-L2 including the amino acid sequence LASNLES (SEQ ID NO:60), CDR-L3 including the amino acid sequence QHSRELPFT (SEQ ID NO:61), CDR-H1 including the amino acid sequence GFDFSRYWMS (SEQ ID NO:53), CDR-H2 including the amino acid sequence EINPTSSTINFTPSLKD (SEQ ID NO:54), and CDR-H3 including the amino acid sequence GNYYRYGDAMDY (SEQ ID NO:55) is excluded. The next amino acid residues following the CDR-L3 sequence QHSRELPFT (SEQ ID NO:61) in the protein may, for example, be FGQ. The CDR-H2 may, for example, include the amino acid sequence EINPTSSTINFADSVKG (SEQ ID NO:56), EINPTSSTINYADSVKG (SEQ ID NO:57), or EINPTSSTINFVDSVKG (SEQ ID NO:58).

(31) One aspect of the invention provides an immunoglobulin heavy chain variable region or a protein, such as an anti-CD45 antibody or CD45 binding antibody fragment such as a Fab, Fab.sub.2 or scFv molecule, including an immunoglobulin heavy chain variable region sequence that includes one or more of the amino acid substitutions disclosed in any of the non-parental immunoglobulin heavy chain variable regions disclosed herein versus the parental immunoglobulin heavy chain variable region disclosed herein, in any combination, but otherwise includes the same amino acid sequence as the parental immunoglobulin heavy chain variable region sequence.

(32) One aspect of the invention provides an immunoglobulin light chain variable region or a protein, such as an anti-CD45 antibody or CD45 binding antibody fragment such as a Fab, Fab.sub.2 or scFv molecule, including an immunoglobulin light chain variable region that includes one or more of the amino acid substitutions disclosed in any of the non-parental immunoglobulin light chain variable regions disclosed herein versus the parental immunoglobulin light chain variable region disclosed herein, in any combination, but otherwise includes the same amino acid sequence as the parental immunoglobulin light chain variable region sequence.

(33) One aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45 binding antibody fragment such as a Fab, Fab.sub.2 or scFv molecule, including: (i) an immunoglobulin heavy chain variable region that includes one or more of the amino acid substitutions disclosed in any of the non-parental immunoglobulin heavy chain variable regions disclosed herein versus the parental immunoglobulin heavy chain variable region disclosed herein, in any combination, but otherwise includes the same amino acid sequence as the parental immunoglobulin heavy chain variable region sequence; and (ii) an immunoglobulin light chain variable region that includes one or more of the amino acid substitutions disclosed in any of the non-parental immunoglobulin light chain variable regions disclosed herein versus the parental immunoglobulin light chain variable region disclosed herein, in any combination, but otherwise includes the same amino acid sequence as the parental immunoglobulin light chain variable region sequence.

(34) One aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45 binding antibody fragment such as a Fab, Fab.sub.2 or scFv molecule, including: (i) the parental immunoglobulin heavy chain variable region disclosed herein, or an immunoglobulin heavy chain variable region that includes one or more of the amino acid substitutions disclosed in any of the non-parental immunoglobulin heavy chain variable regions disclosed herein versus the parental immunoglobulin heavy chain variable region disclosed herein, in any combination, but otherwise includes the same amino acid sequence as the parental immunoglobulin heavy chain variable region sequence; and (ii) the parental immunoglobulin light chain variable region disclosed herein, or an immunoglobulin light chain variable region that includes one or more of the amino acid substitutions disclosed in any of the non-parental immunoglobulin light chain variable regions disclosed herein versus the parental immunoglobulin light chain variable region disclosed herein, in any combination, but otherwise includes the same amino acid sequence as the parental immunoglobulin heavy chain variable region sequence. provided that the protein does not include both the parental immunoglobulin heavy chain variable region disclosed herein and the parental immunoglobulin light chain variable region disclosed herein.

(35) In one variation of this aspect, the protein does not include the parental immunoglobulin heavy chain variable region disclosed herein and does not include the parental immunoglobulin light chain variable region disclosed herein.

(36) One aspect of the invention provides an immunoglobulin heavy chain variable region or a protein, such as an anti-CD45 antibody or CD45 binding antibody fragment such as a Fab, Fab.sub.2 or scFv molecule, including an immunoglobulin heavy chain variable region sequence that includes the amino acid sequence:

(37) TABLE-US-00002 (SEQIDNO:78) EVKLLESGGGLVQPGGSLKLSCAASGFDFSRYWMX.sub.1WVRQAPGKGLEWX.sub.2 X.sub.3X.sub.4INPTSSTIX.sub.5X.sub.6X.sub.7X.sub.8SLKX.sub.9X.sub.10X.sub.11X.sub.12ISRDNX.sub.13KNX.sub.14LYL QMSX.sub.15X.sub.16RX.sub.17EDTAX.sub.18YYCARGNYYRYGDAMDYWGQGTX.sub.19VTVSS,
wherein X.sub.1 is S or N, X.sub.2 is I or V, X.sub.3 is G, S or A, X.sub.4 is E, Y, A, N, or S, X.sub.5 is N or Y, X.sub.6 is F or Y, X.sub.7 is T, A, or V, X.sub.8 is P or D, X.sub.9 is D or G, X.sub.10 is K or R, X.sub.11 is V or F, X.sub.12 is F or T, X.sub.13 is A or S, X.sub.14 is T or S, X.sub.15 is K or S, X.sub.16 is V or L, X.sub.17 is S or A X.sub.18 is L or V, and X.sub.19 is S or M,
provided that the amino acid sequence does not include the sequence

(38) TABLE-US-00003 (SEQIDNO:2) EVKLLESGGGLVQPGGSLKLSCAASGFDFSRYWMSWVRQAPGKGLEWIGE INPTSSTINFTPSLKDKVFISRDNAKNTLYLQMSKVRSEDTALYYCARGN YYRYGDAMDYWGQGTSVTVSS.
The heavy chain variable region amino acid sequence of this aspect may be referred to herein as the Modified Heavy Chain Variable Region Sequence or the M.H.C.V.R. Sequence.

(39) One aspect of the invention provides an immunoglobulin light chain variable region or a protein, such as an anti-CD45 antibody or CD45 binding antibody fragment such as a Fab, Fab.sub.2 or scFv molecule, including an immunoglobulin light chain variable region sequence that includes the amino acid sequence:

(40) TABLE-US-00004 (SEQIDNO:79) DIX.sub.L1X.sub.L2TQSPX.sub.L3X.sub.L4LX.sub.L5X.sub.L6SX.sub.L7GX.sub.L8RX.sub.L9TX.sub.L10X.sub.L11C X.sub.L32X.sub.L13SKSVSTSGYSYLX.sub.L14WYQQKPGQX.sub.L15PX.sub.L16LLIYLA SX.sub.L17X.sub.L18X.sub.L19X.sub.L20GX.sub.L21PX.sub.L22RFSGSGSGTDFTLX.sub.L23I X.sub.L24X.sub.L25X.sub.L26X.sub.L27X.sub.L28EDX.sub.L29AX.sub.L30YYCQHSRELPFTFG X.sub.L31GTKLEIK,
wherein X.sub.L1 is D or E, X.sub.L2 is A or V or Q, X.sub.L3 is L or M, X.sub.L4 is A or D or S, X.sub.L5 is S or T, X.sub.L6 is A or S, X.sub.L7 is V or L or A, X.sub.L8 is L or P or V, X.sub.L9 is Q or E or D, X.sub.L10 is A or V, X.sub.L11 is I or L, X.sub.L12 is S or N or T, X.sub.L13 is A or S, X.sub.L14 is H, A, S, or N, X.sub.L15 is P or A, X.sub.L16 is K or R, X.sub.L17 is N or T or S, X.sub.L18 is L or R, X.sub.L19 is E or A or Q, X.sub.L20 is S or T, X.sub.L21 is V or I, X.sub.L22 is A or D or S, X.sub.L23 is N or T, X.sub.L24 is H or S, X.sub.L25 is P or S, X.sub.L26 is V or L, X.sub.L27 is E or Q, X.sub.L28 is E or A or P, X.sub.L29 is A or V or F, X.sub.L30 is T or V, X.sub.L31 is S or Q, and X.sub.L32 is R or K
provided that the amino acid sequence does not include the sequence

(41) TABLE-US-00005 (SEQIDNO:28) DIALTQSPASLAVSLGQRATISCRASKSVSTSGYSYLHWYQQKPGQPPKL LIYLASNLESGVPARFSGSGSGTDFTLNIHPVEEEDAATYYCQHSRELPF TFGSGTKLEIK.
The light chain variable region amino acid sequence of this aspect may be referred to herein as the Modified Light Chain Variable Region Sequence or the M.L.C.V.R. Sequence.

(42) One aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45 binding antibody fragment such as a Fab, Fab.sub.2 or scFv molecule, including: (i) a heavy chain variable region including the M.H.C.V.R. Sequence or the parental heavy chain variable region amino sequence disclosed herein; and (ii) a light chain variable region including the M.L.C.V.R. Sequence or the parental light chain variable region amino sequence disclosed herein,
provided that the protein does not include both the parental heavy chain variable region amino sequence disclosed herein and the parental light chain variable region amino sequence disclosed herein.

(43) One aspect of the invention provides a protein, such as an anti-CD45 antibody or CD45 binding antibody fragment such as a Fab, Fab.sub.2 or scFv molecule, including: (i) a heavy chain variable region including the M.H.C.V.R. Sequence; and (ii) a light chain variable region including the M.L.C.V.R. Sequence.

(44) One aspect of the invention provides a protein, such as a CD45 binding scFv molecule or a protein including at least one scFv segment, that includes a consecutive (amino to carboxy terminal order) amino acid sequence that includes amino acids 1-120 (the amino acids through and including the sequence VTVS or corresponding amino acid positions) or amino acids 1-121 of a heavy chain variable region as disclosed herein, followed by a linker amino acid sequence, followed by the amino acid sequence of a light chain variable region disclosed herein, provided that the protein does not include both amino acids 1-120 of the parental heavy chain variable region sequence disclosed herein and the parental light chain variable region sequence disclosed herein. In one variation, the protein does not include either of amino acids 1-120 of the parental heavy chain variable region sequence disclosed herein and the parental light chain variable region sequence disclosed herein.

(45) The linker amino acid sequence may, for example, include any of the amino acid sequences: KISGGGGSGGGGSGGGGSGGGGSGGGGSS (SEQ ID NO:80); SPNSASHSGSAPQTSSAPGSQ (SEQ ID NO:81); (G3S).sub.n where, for example, n is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, such as (G3S).sub.4, i.e., GGGSGGGSGGGSGGGS (SEQ ID NO:82); or (G4S).sub.n, i.e., (GGGGS [SEQ ID NO:83]).sub.n where, for example, n is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, such as (G4S).sub.5, i.e., GGGGSGGGGSGGGGSGGGGSGGGGS, (SEQ ID NO:84).

(46) The heavy chain variable region and light chain variable regions disclosed herein each include three (3) complementarity determining regions (CDRs). The CDRs are surrounded by immunoglobulin framework regions (FRs) in the following manner: FR1 is the amino acid sequence preceding (N-terminal to) CDR1, FR2 is the amino acid sequence between CDR1 and CDR2, FR3 is the amino acid sequence between CDR2 and CDR3, and FR4 is the amino acid sequence following (C-terminal to) CDR3 to the end of the variable region sequence.

(47) As used herein, the term antibody fragment includes without limitation proteolytic fragments of antibodies, such as Fab or Fab.sub.2 (F(ab).sub.2) fragments, recombinant antibody fragments with covalently associated or non-covalently associated chains, scFv molecules, and rIgG fragments (rIgG refers to reduced IgG (?75,000 daltons)) also known as half-IgG.

(48) The proteins, such as antibodies and antibody fragments, may, for example, be linked directly or indirectly, via a chemically conjugated chelator, to a radionuclide, for example, to target cytotoxic radiation to CD45-expressing cells in mammalian subject such as a human patient, or to non-cytotoxically image CD45-expression in a mammalian subject such as a human patient. For example, the antibody may be directly labeled with .sup.131I according to the methods disclosed in U.S. Pat. No. 10,420,851 or the antibody may be chemically conjugated to a chelator, such as p-SCN-DOTA and labeled with a radionuclide .sup.225Ac, according to the procedures described in U.S. Pat. No. 9,603,954.

(49) The radionuclide may, for example, be selected from .sup.131I, .sup.125I, .sup.123I, .sup.32P, .sup.213Po, .sup.134Ce, .sup.43Sc, .sup.44Sc, .sup.47Sc, .sup.55Co, .sup.60Cu, .sup.61Cu, .sup.62Cu, .sup.64Cu, .sup.67Cu, .sup.66Ga, .sup.67Ga, .sup.68Ga, .sup.82Rb, .sup.86Y, .sup.87Y, .sup.90Y, .sup.89Zr, .sup.97Ru, .sup.105Rh, .sup.109Pd, .sup.111In, .sup.117mSn, .sup.149Pm, .sup.149Tb, .sup.161Tb, .sup.153Sm, .sup.177Lu, .sup.186Re, .sup.188Re, .sup.199Au, .sup.201Tl, .sup.137Cs, .sup.223Ra, .sup.203Pb, .sup.212Pb, .sup.211At, .sup.212Bi, .sup.213Bi, .sup.225Ac, and .sup.227Th.

(50) The chelator group in the various aspects of the invention may, for example, include: 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DO3A) or a derivative thereof; 1,4,7-triazacyclononane-1,4-diacetic acid (NODA) or a derivative thereof; 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) or a derivative thereof; 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) or a derivative thereof; 1,4,7-triazacyclononane, 1-glutaric acid-4,7-diacetic acid (NODAGA) or a derivative thereof; 1,4,7,10-tetraazacyclodecane, 1-glutaric acid-4,7,10-triacetic acid (DOTAGA) or a derivative thereof; 1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetraacetic acid (TETA) or a derivative thereof; 1,4,8,11-tetraazabicyclo[6.6.2]hexadecane-4,11-diacetic acid (CB-TE2A) or a derivative thereof; diethylene triamine pentaacetic acid (DTPA), its diester, or a derivative thereof; 2-cyclohexyl diethylene triamine pentaacetic acid (CHX-A-DTPA) or a derivative thereof; deforoxamine (DFO) or a derivative thereof; 1,2-[[6-carboxypyridin-2-yl]methylamino]ethane (H.sub.2dedpa) or a derivative thereof; DADA or a derivative thereof; 1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetra(methylene phosphonic acid) (DOTP) or a derivative thereof; 4-amino-6-[[16-[(6-carboxypyridin-2-yl)methyl]-1,4,10,13-tetraoxa-7,16-diazacyclooctadec-7-yl]methyl]pyridine-2-carboxylic acid (MACROPA-NH.sub.2) or a derivative thereof; MACROPA or a derivative thereof; 1,4,7,10-tetrakis(carbamoylmethyl)-1,4,7,10-tetraazacyclododecane (TCMC) or a derivative thereof; {4-[2-(bis-carboxymethylamino)-ethyl]-7-carboxymethyl-[1,4,7]triazonan-1-yl}-acetic acid (NETA) or a derivative thereof; Diamsar or a derivative thereof; 1,4,7-triazacyclononane-1,4,7-tris[methyl(2-carboxyethyl)phosphinic acid (TRAP, PRP9, TRAP-Pr) or a derivative thereof; N,N-bis(6-carboxy-2-pyridylmethyl)ethylenediamine-N,N-diacetic acid (H4octapa) or a derivative thereof; N,N-[1-benzyl-1,2,3-triazole-4-yl]methyl-N,N-[6-(carboxy)pyridin-2-yl]-1,2-diaminoethane (H2azapa) or a derivative thereof; N,N-[[6-(carboxy)pyridin-2-yl]methyl]diethylenetriamine-N,N,N-triacetic acid (H5decapa) or a derivative thereof; N,N-bis(2-hydroxy-5-sulfobenzyl)ethylenediamine-N,N-diacetic acid (SHBED) or a derivative thereof; N,N-bis(2-hydroxybenzyl)ethylenediamine-N,N-diacetic acid (HBED) or a derivative thereof; 3,6,9,15-tetraazabicyclo[9.3.1]pentadeca-1(15),11,13-triene-3,6,9-triacetic acid (PCTA) or a derivative thereof; desferrioxamine B (DFO) or a derivative thereof; N,N-(methylenephosphonate)-N,N-[6-(methoxycarbonyl)pyridin-2-yl]methyl-1,2-diaminoethane (H6phospa) or a derivative thereof; 1,4,7,10,13,16-hexaazacyclohexadecane-N,N,N,N,N,N-hexaacetic acid (HEHA) or a derivative thereof; 1,4,7,10,13-pentaazacyclopentadecane-N,N,N,N,N-pentaacetic acid (PEPA) or a derivative thereof; or 3,4,3-LI(1,2-HOPO) or a derivative thereof.

(51) The proteins, such as antibodies and antibody fragments, may, for example, be linked to one or more cytotoxic drugs to target and deplete CD45-expressing cells in a mammalian subject such as a human patient. Thus, one aspect of the invention provides an antibody-drug-conjugate (ADC) that includes an antibody or antigen-binding antibody fragment according to the invention as a component. The ADC may, for example, be a conjugate with a cytotoxin containing a benzodiazepine moiety, for example. a pyrrolobenzodiazepine (PBD) or an indolinobenzodiazepine (IGN) moiety, as disclosed in U.S. Pub. No. 20220175951.

(52) The words comprising and forms of the word comprising as well as the word including and forms of the word including, as used in this description and in the claims, do not limit the inclusion of elements beyond what is referred to. Additionally, although throughout the present disclosure various aspects or elements thereof are described in terms of including or comprising, corresponding aspects or elements thereof described in terms of consisting essentially of or consisting of are similarly disclosed. For example, while certain aspects of the invention have been described in terms of a protein including or comprising one or more particular amino acid sequences, corresponding aspects instead reciting a protein consisting essentially of or consisting of the one or more particular amino acid sequences are also within the scope of said aspects and disclosed by this disclosure.

(53) In addition, compositions including a radiolabeled antibody may include one or more pharmaceutically acceptable carriers or pharmaceutically acceptable excipients. Such carriers are well known to those skilled in the art. For example, injectable drug delivery systems include solutions, suspensions, gels, microspheres and polymeric injectables, and can include excipients such as solubility-altering agents (e.g., ethanol, propylene glycol and sucrose) and polymers (e.g., polycaprolactones and PLGA's). An exemplary formulation may be as substantially described in U.S. Pat. No. 10,420,851 or International Pub. No. WO 2017/155937, incorporated by reference herein. For example, according to certain aspects, the formulation may include 0.5% to 5.0% (w/v) of an excipient selected from the group consisting of ascorbic acid, polyvinylpyrrolidone (PVP), human serum albumin (HSA), a water-soluble salt of HSA, and mixtures thereof. Certain formulations may include 0.5-5% ascorbic acid; 0.5-4% polyvinylpyrrolidone (PVP); and the monoclonal antibody in 50 mM PBS buffer, pH 7.

(54) The humanized and various anti-huCD45 antibodies disclosed herein may, for example, be labeled with a radionuclide, such as .sup.131I, .sup.177Lu, .sup.211At, .sup.227Th or .sup.225Ac, or conjugated to a cytotoxic drug, for use as a conditioning agent in preparation of a bone marrow transplant (BMT) or of a hematopoietic stem cell transplant (HSCT), for example, for the treatment of a hematological cancer, or in preparation of administration of a genetically engineered cell therapy such as a CAR-T therapy for the treatment of a cancer, or for use as a direct treatment of a hematological cancer, such as a myeloid or lymphoid hematological cancer. such as a leukemia, for example acute myeloid leukemia (AML), or a lymphoma, for example non-Hodgkin lymphoma (NHL), or for use as an immunological resetting agent in the treatment of an autoimmune disease such as multiple sclerosis, or otherwise in the treatment of an autoimmune disease. The hematological cancer or disorder treated may, for example, be a leukemia (such as acute myeloid leukemia (AML), acute promyelocytic leukemia, acute lymphoblastic leukemia (ALL), acute mixed lineage leukemia, chronic myeloid leukemia (CML), chronic lymphocytic leukemia (CLL), hairy cell leukemia, or large granular lymphocytic leukemia), myelodysplastic syndrome (MDS), a myeloproliferative disorder (such as polycythemia vera, essential thrombocytosis, primary myelofibrosis or chronic myeloid leukemia), multiple myeloma, MGUS and similar disorders, a lymphoma (such as Hodgkin's lymphoma (HL), non-Hodgkin lymphoma (NHL), primary mediastinal large B-cell lymphoma, diffuse large B-cell lymphoma, follicular lymphoma, transformed follicular lymphoma, splenic marginal zone lymphoma, lymphocytic lymphoma, or T-cell lymphoma), or another B-cell malignancy. In one aspect, the radiolabeled or drug-conjugated anti-huCD45 antibody is administered as a maintenance therapy to a subject who previously received a BMT or HSCT in treatment of a hematological cancer such as AML or another described herein, for example, to treat or to prevent a relapse or recurrence of the cancer. Further, the humanized and various anti-huCD45 antibodies disclosed herein may, for example, be labeled with a radionuclide, such as .sup.131I, .sup.177Lu, .sup.211At, .sup.227Th or .sup.225Ac, or conjugated to a cytotoxic drug, for use, such as in a non-myeloablative dose, in the prevention or treatment of graft versus host disease (GVHD) or other immunological intolerance to the transplanted organ/tissue and, more generally, to promote immunological tolerance of a transplanted solid organ or solid organ tissue by the host, in a solid organ transplant setting. In one aspect, a non-myeloablative amount of a radiolabeled anti-huCD45 antibody as disclosed herein is administered to a human solid organ transplant patient after the solid organ transplantation, in order to the prevent, reduce, or treat graft versus host disease (GVHD) or other immunological intolerance and, more generally, to promote immunological tolerance of a transplanted solid organ in a host. In each case described here, the subject may be a mammal such as a human patient.

(55) Another aspect of the invention provides use of a radiolabeled anti-huCD45 antibody as disclosed herein in the preparation of a medicament for one or more of (i) myeloablative conditioning in a human patient such as in preparation for a bone marrow transplant or a hematopoietic stem cell transplant, (ii) non-myeloablative conditioning such as for preparation of a human patient to receive cell therapy in the treatment of a hematological malignancy or solid tumor cancer, such as a CAR-T, recombinant TCR T-cell, or CAR-NK cell therapy, or receive cell therapy in the treatment of a non-cancerous (non-malignant) genetic disorder such as ?-thalassemia or sickle cell anemia, (iii) direct treatment of a hematological cancer in a human patient such as any of those described above, (iv) use, for example, as an immunological resetting agent, in the treatment of an autoimmune disease such as multiple sclerosis in a human patient, and (v) use in preventing, reducing, or treating graft versus host disease (GVHD) or other immunological intolerance and, more generally, to promote immunological tolerance of a transplanted solid organ in a human patient. The CAR-T cell therapy may, for example, include Kymriah? (tisagenlecleucel; target CD19), Yescarta? (axicabtagene ciloleucel; target CD19), Tecartus? (brexucabtagene autoleucal; target CD19), Breyanzi? (lisocabtagene maraleucel; target CD19), Abecma? (idecabtagene vicleucel; target BCMA), or Carvykti? (ciltacabtagene autoleucel; target BCMA).

Example 1: Production of a Radiolabeled Chelator-Conjugated Anti-huCD45 Antibody

(56) Conjugation to a chelator: A vial of lyophilized p-SCN-Bn-DOTA is reconstituted with metal-free water to a concentration of 10 mg/ml. To the actinium reaction vial, 0.02 ml of ascorbic acid solution (150 mg/ml) and 0.05 ml of reconstituted p-SCN-Bn-DOTA are added and the pH adjusted to between 5 and 5.5 with 2M tetramethylammonium acetate (TMAA). The mixture is then heated at 55?4? C. for 30 minutes.

(57) To determine the labeling efficiency of the .sup.225Ac-p-SCN-Bn-DOTA, an aliquot of the reaction mixture is removed and applied to a 1 ml column of Sephadex C25 cation exchange resin. The product is eluted in 2-4 ml fractions with a 0.9% saline solution. The fraction of .sup.225Ac activity that elutes is .sup.225Ac-p-SCN-Bn-DOTA and the fraction that is retained on the column is un-chelated, unreactive .sup.225Ac. Typically, the labeling efficiency is greater than 95%.

(58) To the reaction mixture, 0.22 ml of previously prepared anti-CD45 mAb in DTPA (1 mg) and 0.02 ml of ascorbic acid are added. The DTPA is added to bind any trace amounts of metals that may compete with the labeling of the antibody. The ascorbic acid is added as a radio-protectant. The pH is adjusted with carbonate buffer to pH 8.5-9. The mixture is heated at 37?3? C. for 30 minutes.

(59) The final product may be purified by size exclusion chromatography using 10DG resin and eluted with 2 ml of 1% HSA.

(60) Radiolabeling: The antibody may be conjugated to a linker, such as any of the linkers described in the above indicated patent applications. An exemplary linker includes at least dodecane tetraacetic acid (DOTA), wherein a goal of the conjugation reaction is to achieve a DOTA-antibody ratio of 3:1 to 5:1. Chelation with the radionuclide, such as .sup.177Lu, .sup.90Y, or .sup.225Ac may then be performed and efficiency and purity of the resulting radiolabeled antibody, such as an anti-CD45 antibody, may be determined by HPLC and iTLC.

(61) An exemplary labeling reaction for .sup.225Ac is as follows: A reaction including 15 ?l 0.15M NH.sub.4OAc buffer, pH=6.5 and 2 ?L (10 ?g) DOTA-anti-CD45 (5 mg/ml) may be mixed in an Eppendorf reaction tube, and 4 ?L .sup.225Ac (10 ?Ci) in 0.05 M HCl subsequently added. The contents of the tube may be mixed with a pipette tip and the reaction mixture incubated at 37? C. for 90 min with shaking at 100 rpm. At the end of the incubation period, 3 ?L of a 1 mM DTPA solution may be added to the reaction mixture and incubated at room temperature for 20 min to bind the unreacted .sup.225Ac into the .sup.225Ac-DTPA complex. Instant thin layer chromatography with 10 cm silica gel strip and 10 mM EDTA/normal saline mobile phase may be used to determine the radiochemical purity of .sup.225Ac-DOTA-anti-CD45 through separating .sup.225Ac-labeled anti-CD45 (.sup.225Ac-DOTA-anti-CD45) from free .sup.225Ac (.sup.225Ac-DTPA). In this system, the radiolabeled antibody stays at the point of application and .sup.225Ac-DTPA moves with the solvent front. The strips may be cut in halves and counted in the gamma counter equipped with the multichannel analyzer using channels 72-110 for .sup.225Ac to exclude its daughters.

(62) Purification: An exemplary radiolabeled targeting agent, such as .sup.225Ac-DOTA-antibody, may be purified either on PD10 columns pre-blocked with 1% HSA or on Vivaspin centrifugal concentrators with a 50 kDa MW cut-off with 2?1.5 mL washes, 3 min per spin. HPLC analyses of the .sup.225Ac-DOTA-antibody after purification may be conducted using a Waters HPLC system equipped with flow-through Waters UV and Bioscan Radiation detectors, using a TSK3000SW XL column eluted with PBS at pH=7.4 and a flow rate of 1 ml/min.

Example 2: Radio-Iodination of Anti-huCD45 Antibody and Purification in the Presence of Ascorbic Acid

(63) Anti-CD45 antibodies may be radio-iodinated according to the following method.

(64) One mg of an anti-CD45 antibody is labeled with 20 to 30 mCi of .sup.131INa (30 mCi) in the presence of chloramine-T (23 micrograms) in PBS buffer (pH 7.2). The reaction is quenched with the addition of aqueous sodium thiosulfate (69 micrograms) and diluted with cold NaI (1 mg). Immediately following, a concentrated ascorbic acid solution made in 50 mM PBS (pH 7) is added to achieve 2.5% (w/v) ascorbic acid strength in the quenched reaction mixture. Labeling reactions up to 3,000 mCi per batch may, for example, be successfully performed using this method.

(65) The radiolabeled antibody, such as radiolabeled immunoglobulin, can be purified by gel filtration on a sterile, pre-packed commercially available Sephadex G25 column (GE HiPrep 26/10 column, bed volume 53 mL) using PBS (50 mM, pH 7) mobile phase supplemented with 2.5% (w/v) ascorbic acid to stabilize the radiolabeled product. Up to 1,000 mCi reaction volume can be purified on a single column. The product can be collected in a 5 to 35 mL elution volume from the column.

(66) Radio-iodinated reaction batches of <200 mCi may be purified in a similar fashion on a smaller desalting column (GE PD10 column, bed volume 8.6 mL).

Example 3: Radio-Iodination of Anti-CD45 Immunoglobulin and Purification in the Presence of Ascorbic Acid and HSA

(67) The radio-iodination and purification may be performed essentially as described in Example 2, except that 2% or 4% (w/v) HSA is also added along with 2.5% (w/v) ascorbic acid to the quenched reaction as well as in the elution buffer during the purification process.

(68) Experiments were further performed to characterize the cell binding attributes of the various humanized anti-huCD45 antibodies disclosed herein against three different human AML cell lines, to compare the cell killing performance of selected .sup.225Ac-labeled humanized anti-huCD45 antibodies and .sup.225Ac-labeled parental antibody at different radiation doses, and to evaluate the biodistribution of selected radiolabeled humanized anti-huCD45 antibodies in a murine HL60 xenotransplant tumor model, as described below.

(69) Cell Binding

(70) The cell binding of humanized anti-huCD45 antibody clones was evaluated using flow cytometry. MV411, HL60 or U937 cells were plated at 1?10.sup.6 cells per well and incubated with humanized BC8, chimeric parental mAb (Ch. Parental) or parental mAb (fully murine) at a concentration of 100 ?g/mL and incubated for one hour at 4? C. An anti-human secondary (human secondary) antibody or anti-mouse secondary (mouse secondary) antibody was added to the cells and incubated for thirty minutes at 4? C. Cell binding mean fluorescence intensity (MFI) was detected by measuring the cell staining signal with a BD Accuri C6 flow cytometer and data analyzed using BD Accuri software. The tested humanized anti-huCD45 mAbs are identified in the figures with h followed first by the humanized heavy chain identification number and then the humanized light chain identification number. IgG indicated non-specific immunoglobulin primary antibody control. The chimeric parental anti-huCD45 mAb (Ch. Parental) having the murine variable regions of the parental antibody and human constant regions was prepared in order to provide more direct comparisons in experiments using anti-human secondary antibody.

(71) FIG. 11 shows cell binding data for binding of various humanized anti-huCD45 antibodies disclosed herein to MV411 human AML cell line cells, versus controls.

(72) FIG. 12 shows cell binding data for binding of various humanized anti-huCD45 antibodies disclosed herein to U937 human pro-monocytic myeloid leukemia cell line cells, versus controls.

(73) FIG. 13 shows cell binding data for binding of various humanized anti-huCD45 antibodies disclosed herein to HL60 human, promyelocytic AML cell line cells, versus a modified chimeric parental control antibody.

(74) Cell Viability

(75) To determine cell viability effects, HL60 cells were plated at 1?10.sup.5 cells per well and two selected humanized anti-hu CD45 mAbs (h 3-3 and h 3-4, from two different lots each) or parental mAb (fully murine) were added to the cells at a concentration range of 1 to 1,000 nCi/mL (Actinium-225 radiolabeled, 1, 10, 100 and 1,000 nCi/mL tested) and 0.00163 ?g/mL to 7.84 ?g/mL (non-radiolabeled). After incubating at 37? C. for 72 hours, the cells were washed with PBS and stained with BD Via-Probe Red Nucleic Acid Stain. Cell viability was detected by measuring the cell staining signal with a BD Accuri C6 flow cytometer and data analyzed using GraphPad Prism software. FIG. 14 shows the resulting cell viability data. The non-radiolabeled humanized and parental antibodies had little or no negative effect on cell viability while the corresponding .sup.225Ac-labeled antibodies increasingly and significantly reduced cell viability with increasing radiation dose.

(76) Biodistribution

(77) BALB/c nude mice bearing human HL60 subcutaneous tumor xenografts were prepared and dosed with Indium-111 radiolabeled humanized anti-huCD45 mAbs (h 3-3 and h 3-4) or parental anti-huCD45 mAb at 50 ?Ci (2-3 mice per group). After 24, 120 and 168 hours, the blood, tumors, and organ tissues were harvested, weighed and measured for radioactivity. The percentage injected dose per gram tissue (% ID/g) was determined and data analyzed using GraphPad Prism software. The biodistribution data is shown in Table 2 below.

(78) TABLE-US-00006 TABLE 2 % ID/g Parental HC3 + LC3 HC3 + LC4 Organ Mean SD Mean SD Mean SD Blood 5.893 2.612 4.693 2.984 2.695 0.898 Brain 0.193 0.040 0.127 0.050 0.085 0.021 Heart 1.973 0.959 1.563 0.765 1.000 0.269 Lung 3.517 1.135 2.943 1.075 1.935 0.530 Spleen 7.233 0.976 7.297 3.863 6.535 0.064 Kidneys 3.073 0.682 3.043 0.827 2.770 0.297 Muscle 0.560 0.265 0.500 0.115 0.250 0.000 Bone 1.497 0.510 1.737 0.753 2.100 1.188 Tumor 15.083 2.527 11.580 5.240 10.180 0.764 Stomach 0.537 0.222 0.380 0.085 0.450 0.127 Small Intestine 0.793 0.407 0.590 0.208 0.845 0.092 Large Intestine 0.553 0.164 0.380 0.184 0.570 0.127 Liver 8.557 0.379 5.790 2.786 8.710 0.113

(79) These data show that the tested humanized anti-huCD45 antibodies and the parental anti-huCD45 antibody accumulate strongly and comparably in the HL60 tumor and have comparable and expected biodistribution among the organs.

(80) The radiolabeled antibodies used in the preceding experiments were prepared by chemical conjugation of the respective antibodies to the bifunctional chelator p-SCN-Bn-DOTA (S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid; Catalog. No. B-205, Macrocyclics, Inc., Plano, Texas, USA) followed by radiolabeling of the conjugate by chelation of the radionuclide, .sup.225Ac or .sup.111In, to the DOTA moiety of the respective conjugate.

(81) While various specific embodiments have been illustrated and described herein, it will be appreciated that various changes can be made without departing from the spirit and scope of the invention(s). Moreover, features described in connection with one aspect of the invention may be used in conjunction with other aspects of the invention, even if not explicitly exemplified in combination within.